LAWRENCE BARAK

Summary

Affiliation: Duke University Medical Center
Country: USA

Publications

  1. ncbi request reprint N-terminal tyrosine modulation of the endocytic adaptor function of the beta-arrestins
    Sébastien Marion
    Department of Cell Biology, Duke University, Durham, North Carolina 27710, USA
    J Biol Chem 282:18937-44. 2007
  2. ncbi request reprint A beta-arrestin binding determinant common to the second intracellular loops of rhodopsin family G protein-coupled receptors
    Sébastien Marion
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 281:2932-8. 2006
  3. pmc Triphenylmethane dye activation of beta-arrestin
    Larry S Barak
    Departments of Cell Biology, Duke University, Durham, NC 27710, USA
    Biochemistry 52:5403-14. 2013
  4. pmc Polyclonal HER2-specific antibodies induced by vaccination mediate receptor internalization and degradation in tumor cells
    Xiu Rong Ren
    Department of Medicine, Duke University Medical Center, 595 LaSalle Street, Durham, NC 27710, USA
    Breast Cancer Res 14:R89. 2012
  5. pmc Modeling of bias for the analysis of receptor signaling in biochemical systems
    Larry S Barak
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, United States
    Biochemistry 51:1114-25. 2012
  6. ncbi request reprint G protein-coupled receptor desensitization as a measure of signaling: modeling of arrestin recruitment to activated CCK-B receptors
    Larry S Barak
    Departments of Cell Biology and Medicine, Duke University, Durham, NC 27710, USA
    Assay Drug Dev Technol 1:409-24. 2003
  7. ncbi request reprint Dishevelled 2 recruits beta-arrestin 2 to mediate Wnt5A-stimulated endocytosis of Frizzled 4
    Wei Chen
    Howard Hughes Medical Institute, Departments of Medicine and Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
    Science 301:1391-4. 2003
  8. ncbi request reprint Human substance P receptor lacking the C-terminal domain remains competent to desensitize and internalize
    Mark D Richardson
    Departments of Anesthesiology and Cell Biology, The Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    J Neurochem 84:854-63. 2003
  9. ncbi request reprint Beta-arrestin 2 regulates zebrafish development through the hedgehog signaling pathway
    Alyson M Wilbanks
    Department of Cell Biology, Center for Models of Human Disease, Institute for Genome Science and Policy, Duke University Medical Center, Durham, NC 27710, USA
    Science 306:2264-7. 2004
  10. pmc Pharmacological characterization of membrane-expressed human trace amine-associated receptor 1 (TAAR1) by a bioluminescence resonance energy transfer cAMP biosensor
    Larry S Barak
    Box 3287, Duke University, Durham, NC 27710
    Mol Pharmacol 74:585-94. 2008

Research Grants

Collaborators

Detail Information

Publications19

  1. ncbi request reprint N-terminal tyrosine modulation of the endocytic adaptor function of the beta-arrestins
    Sébastien Marion
    Department of Cell Biology, Duke University, Durham, North Carolina 27710, USA
    J Biol Chem 282:18937-44. 2007
    ..Additionally, these naturally occurring variations in beta-arrestins may also differentially regulate the composition of the signaling complexes organized on the receptor...
  2. ncbi request reprint A beta-arrestin binding determinant common to the second intracellular loops of rhodopsin family G protein-coupled receptors
    Sébastien Marion
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 281:2932-8. 2006
    ....
  3. pmc Triphenylmethane dye activation of beta-arrestin
    Larry S Barak
    Departments of Cell Biology, Duke University, Durham, NC 27710, USA
    Biochemistry 52:5403-14. 2013
    ..S. and Europe. Our results indicate triphenylmethane dyes as a result of novel pharmacology may have additional roles as β-arrestin/clathrin pathway signaling modulators in both pharmacology research and clinical therapy. ..
  4. pmc Polyclonal HER2-specific antibodies induced by vaccination mediate receptor internalization and degradation in tumor cells
    Xiu Rong Ren
    Department of Medicine, Duke University Medical Center, 595 LaSalle Street, Durham, NC 27710, USA
    Breast Cancer Res 14:R89. 2012
    ..Thus, abrogation of persistent HER2 expression at the plasma membrane to synergize with current approaches may represent a novel therapeutic strategy...
  5. pmc Modeling of bias for the analysis of receptor signaling in biochemical systems
    Larry S Barak
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, United States
    Biochemistry 51:1114-25. 2012
    ....
  6. ncbi request reprint G protein-coupled receptor desensitization as a measure of signaling: modeling of arrestin recruitment to activated CCK-B receptors
    Larry S Barak
    Departments of Cell Biology and Medicine, Duke University, Durham, NC 27710, USA
    Assay Drug Dev Technol 1:409-24. 2003
    ..In addition to its use in drug screening, this methodology should generalize to other receptors for use in diagnosis and monitoring of bioactive ligands involved in GPCR-based disease...
  7. ncbi request reprint Dishevelled 2 recruits beta-arrestin 2 to mediate Wnt5A-stimulated endocytosis of Frizzled 4
    Wei Chen
    Howard Hughes Medical Institute, Departments of Medicine and Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
    Science 301:1391-4. 2003
    ..These findings provide a previously unrecognized mechanism for receptor recruitment of beta-arrestin and demonstrate that Dvl plays an important role in the endocytosis of frizzled, as well as in promoting signaling...
  8. ncbi request reprint Human substance P receptor lacking the C-terminal domain remains competent to desensitize and internalize
    Mark D Richardson
    Departments of Anesthesiology and Cell Biology, The Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    J Neurochem 84:854-63. 2003
    ..We conclude that truncated hSPR undergoes agonist-dependent desensitization and internalization without detectable receptor phosphorylation...
  9. ncbi request reprint Beta-arrestin 2 regulates zebrafish development through the hedgehog signaling pathway
    Alyson M Wilbanks
    Department of Cell Biology, Center for Models of Human Disease, Institute for Genome Science and Policy, Duke University Medical Center, Durham, NC 27710, USA
    Science 306:2264-7. 2004
    ..These results suggest that a functional interaction between beta-arrestin 2 and Smoothened may be critical to regulate Hh signaling in zebrafish development...
  10. pmc Pharmacological characterization of membrane-expressed human trace amine-associated receptor 1 (TAAR1) by a bioluminescence resonance energy transfer cAMP biosensor
    Larry S Barak
    Box 3287, Duke University, Durham, NC 27710
    Mol Pharmacol 74:585-94. 2008
    ....
  11. pmc Development of small molecules targeting the Wnt pathway for the treatment of colon cancer: a high-throughput screening approach
    Wei Chen
    Dept of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Am J Physiol Gastrointest Liver Physiol 299:G293-300. 2010
    ..In particular, we summarize the results of a screen of over 1,200 drug and druglike compounds we recently completed in which niclosamide was identified as a Wnt pathway antagonist...
  12. ncbi request reprint Activity-dependent internalization of smoothened mediated by beta-arrestin 2 and GRK2
    Wei Chen
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Science 306:2257-60. 2004
    ..A Smo agonist stimulated and a Smo antagonist (cyclopamine) inhibited both phosphorylation of Smo by GRK2 and interaction of beta-arrestin 2 with Smo. beta-Arrestin 2 and GRK2 are thus potential mediators of signaling by activated Smo...
  13. ncbi request reprint Phosphorylation of the platelet-derived growth factor receptor-beta by G protein-coupled receptor kinase-2 reduces receptor signaling and interaction with the Na(+)/H(+) exchanger regulatory factor
    Kerry L Hildreth
    Department of Medicine Cardiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 279:41775-82. 2004
    ..Furthermore, this desensitization appears to involve GRK2-mediated phosphorylation of PDGFRbeta Ser(1104), with consequent dissociation of the PDGFRbeta from NHERF...
  14. pmc Ubiquitination of beta-arrestin links seven-transmembrane receptor endocytosis and ERK activation
    Sudha K Shenoy
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 282:29549-62. 2007
    ....
  15. ncbi request reprint Apparent loss-of-function mutant GPCRs revealed as constitutively desensitized receptors
    Alyson M Wilbanks
    Howard Hughes Medical Institute Laboratories, Departments of Cell Biology and Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Biochemistry 41:11981-9. 2002
    ..Furthermore, constitutive desensitization may underlie some loss-of-function receptor phenotypes and represent an unappreciated mechanism of hormonal resistance...
  16. pmc The anti-helminthic niclosamide inhibits Wnt/Frizzled1 signaling
    Minyong Chen
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Biochemistry 48:10267-74. 2009
    ..e., Frizzled and Dishevelled) and moreover may provide a valuable means of studying the physiological consequences of Wnt signaling...
  17. pmc Identification of select glucocorticoids as Smoothened agonists: potential utility for regenerative medicine
    Jiangbo Wang
    Departments of Medicine, Surgery, and Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 107:9323-8. 2010
    ..Halcinonide, fluticasone, clobetasol, and fluocinonide provide an unprecedented opportunity to develop unique clinical strategies to treat Hedgehog-dependent illnesses...
  18. ncbi request reprint Relationship between the G protein signaling and homologous desensitization of G protein-coupled receptors
    Larry S Barak
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Biochem Biophys Res Commun 339:695-700. 2006
    ..Taken together, our results consistently suggest that G protein signaling and homologous desensitization are independent cellular processes...
  19. ncbi request reprint Constitutive desensitization: a new paradigm for g protein-coupled receptor regulation
    Larry S Barak
    Howard Hughes Medical Institute and Department of Cell Biology, Duke University Medical Center, Durham, North Carolina, USA
    Assay Drug Dev Technol 1:339-46. 2003
    ..We have termed this process constitutive desensitization, and this unexpected receptor property suggests that it may be possible to develop novel classes of signal-inhibiting drugs distinct from conventional antagonists...

Research Grants5

  1. Molecular fingerprinting of GPCR ligands in Cancer
    LAWRENCE BARAK; Fiscal Year: 2005
    ..Moreover, automated imaging systems for the analysis of the biosensors are currently available and their throughput rate for identifying GPCR ligands useful in cancer therapy could be increased upwards of 100 fold. ..
  2. MECHANISMS OF G PROTEIN COUPLED RECEPTOR REGULATION
    LAWRENCE BARAK; Fiscal Year: 1999
    ..Insight gained from them should provide a foundation for the development of new treatment regiments that target diseases such as hypertension, heart failure, and chronic pain syndromes. ..
  3. MECHANISMS OF G PROTEIN COUPLED RECEPTOR REGULATION
    LAWRENCE BARAK; Fiscal Year: 2000
    ..Insight gained from them should provide a foundation for the development of new treatment regiments that target diseases such as hypertension, heart failure, and chronic pain syndromes. ..
  4. MECHANISMS OF G PROTEIN COUPLED RECEPTOR REGULATION
    LAWRENCE BARAK; Fiscal Year: 2001
    ..Insight gained from them should provide a foundation for the development of new treatment regiments that target diseases such as hypertension, heart failure, and chronic pain syndromes. ..
  5. MECHANISMS OF G PROTEIN COUPLED RECEPTOR REGULATION
    LAWRENCE BARAK; Fiscal Year: 2002
    ..Insight gained from them should provide a foundation for the development of new treatment regiments that target diseases such as hypertension, heart failure, and chronic pain syndromes. ..