Affiliation: Duke University Medical Center
- Anti-neutrophil chemokine preserves alveolar development in hyperoxia-exposed newborn ratsR L Auten
Division of Neonatal Medicine, Department of Pediatrics, Neonatal Perinatal Research Institute, Durham, North Carolina 27710, USA
Am J Physiol Lung Cell Mol Physiol 281:L336-44. 2001..Blockade of neutrophil influx in newborns may avert early lung injury and avoid alveolar developmental arrest that contributes to bronchopulmonary dysplasia...
- S-nitrosothiol transport via PEPT2 mediates biological effects of nitric oxide gas exposure in macrophagesMulugu V Brahmajothi
Division of Neonatal Medicine, Department of Pediatrics, Duke University Medical Center, Duke University, Durham, NC 27710, USA
Am J Respir Cell Mol Biol 48:230-9. 2013..The NO gas treatment of alveolar macrophages increased SNO uptake, the activation of sGC, F-actin polymerization, and phagocytosis, and inhibited NF-κB activation, in a manner dependent on SNO transport via PEPT2, as well as via LAT...
- Maternal diesel inhalation increases airway hyperreactivity in ozone-exposed offspringRichard L Auten
Departments of Pediatrics, Duke University, Durham, NC 27710, USA
Am J Respir Cell Mol Biol 46:454-60. 2012..We conclude that maternal inhalation of DE in pregnancy provokes a fetal inflammatory response that, combined with postnatal ozone exposure, impairs alveolar development, and causes a more severe and long-lasting AHR to ozone exposure...
- Maternal exposure to particulate matter increases postnatal ozone-induced airway hyperreactivity in juvenile miceRichard L Auten
Neonatal Medicine, Department of Pediatrics, Duke University, Durham, North Carolina, USA
Am J Respir Crit Care Med 180:1218-26. 2009..Epidemiologic studies implicate air pollutant exposure during pregnancy as a risk factor for wheezing in offspring. Ozone exposure is linked to exacerbations of wheezing in children...
- Hyperoxia impairs postnatal alveolar epithelial development via NADPH oxidase in newborn miceRichard L Auten
Division of Neonatology, Department of Pediatrics, Duke University Medical Center, Neonatal Perinatal Research Institute, Durham, North Carolina 27710, USA
Am J Physiol Lung Cell Mol Physiol 297:L134-42. 2009....
- Inhaled ethyl nitrite prevents hyperoxia-impaired postnatal alveolar development in newborn ratsRichard L Auten
Division of Neonatal Medicine, Department of Pediatrics, Neonatal Perinatal Research Institute, University Medical Center, Durham, North Carolina 27710, USA
Am J Respir Crit Care Med 176:291-9. 2007..Because S-nitrosylation is a key pathway mediating many NO biological effects, treatment with inhaled ENO may better protect postnatal lung development from oxidative stress than NO...
- Transgenic extracellular superoxide dismutase protects postnatal alveolar epithelial proliferation and development during hyperoxiaRichard L Auten
Neonatal Perinatal Research Institute, Department of Pediatrics, Duke University Medical Center, Durham, North Carolina 27710, USA
Am J Physiol Lung Cell Mol Physiol 290:L32-40. 2006..Reducing the 95% O2-induced impairment of epithelial proliferation at a critical window of lung development was associated with protection against DNA damage and preservation of apical T1alpha expression at P3...
- Nonpeptide CXCR2 antagonist prevents neutrophil accumulation in hyperoxia-exposed newborn ratsR L Auten
Department of Pediatrics, Duke University Medical Center, Durham, North Carolina 27710, USA
J Pharmacol Exp Ther 299:90-5. 2001..SB-265610 reduced the antiapoptotic effect of CINC-1 to the levels of those untreated with CINC-1. A selective CXCR2 antagonist may be useful in diseases where neutrophil-mediated exacerbation is present...
- Blocking leukocyte influx and function to prevent chronic lung disease of prematurityRichard L Auten
Neonatal Perinatal Research Institute, Division of Neonatal Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
Pediatr Pulmonol 35:335-41. 2003..Examination of the inflammatory pathways that initiate and propagate lung injury and subsequent abnormal development points to promising new strategies that may one day be tailored to individual patients...
- Antimacrophage chemokine treatment prevents neutrophil and macrophage influx in hyperoxia-exposed newborn rat lungMichael A Vozzelli
Department of Pediatrics, Division of Neonatal Medicine, Neonatal Perinatal Research Institute, Durham, NC 27710, USA
Am J Physiol Lung Cell Mol Physiol 286:L488-93. 2004..Tissue carbonyls, a measure of protein oxidation, were decreased in anti-MCP-1-treated pups. Anti-MCP-1 treatment prevented neutrophil influx and reduced protein oxidation in hyperoxia-exposed newborn rats...
- Blocking neutrophil influx reduces DNA damage in hyperoxia-exposed newborn rat lungRichard L Auten
Neonatal Perinatal Research Institute, Division of Neonatal Medicine, Department of Pediatrics, Duke University Medical Center, Durham, North Carolina 27710, USA
Am J Respir Cell Mol Biol 26:391-7. 2002..Effects of 95% O2 on TUNEL are not primarily due to apoptosis in this model. Neutrophil-mediated oxidative DNA damage may contribute to abnormal lung development in newborns subjected to significant oxidative stress...
- Biochemical effects of ozone on asthma during postnatal developmentRichard L Auten
Department of Pediatrics Neonatal Medicine, Duke University, DUMC Box 3373, Durham, NC 27710, USA
Biochim Biophys Acta 1810:1114-9. 2011..Ozone exposure during early life has the potential to contribute to the development of asthma as well as to exacerbate underlying allergic asthma...
- Extracellular superoxide dismutase protects lung development in hyperoxia-exposed newborn miceMohamed N Ahmed
Departments of Pediatrics, Medicine, Anesthesiology, Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
Am J Respir Crit Care Med 167:400-5. 2003..After 7 days 95% O2 + 14 days 60% O2, lung inflammation measured as myeloperoxidase activity was reduced to control levels in all treatment groups...
- Airway smooth muscle relaxation is impaired in mice lacking the p47phox subunit of NAD(P)H oxidasePasquale Chitano
Dept of Pediatrics and Neonatal Perinatal Research Institute, Duke Univ Medical Center, Rm 302, Bell Bldg, Box 2994, Durham, NC 27710, USA
Am J Physiol Lung Cell Mol Physiol 294:L139-48. 2008..Most importantly, we report the first evidence that the p47phox subunit of NAD(P)H oxidase plays a role in ASM relaxation...
- Pharmacologically augmented S-nitrosylated hemoglobin improves recovery from murine subarachnoid hemorrhageHuaxin Sheng
Department of Anesthesiology, Duke University Medical Center, Durham, NC 27710, USA
Stroke 42:471-6. 2011..This study tested the hypothesis that enhancement of S-nitrosylated hemoglobin by ethyl nitrite inhalation improves outcome after experimental subarachnoid hemorrhage (SAH)...
- Oxygen toxicity and reactive oxygen species: the devil is in the detailsRichard L Auten
Department of Pediatrics, Duke Medical Center, Durham, NC 27710, USA
Pediatr Res 66:121-7. 2009....
- Protection from lipopolysaccharide-induced lung injury by augmentation of airway S-nitrosothiolsHarvey E Marshall
Department of Medicine, Division of Pulmonary, Allergy, and Critical Care Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
Am J Respir Crit Care Med 180:11-8. 2009..Ethyl nitrite (ENO) is a gas with chemical properties favoring SNO formation. Augmentation of airway SNO by inhaled ENO treatment may decrease lung inflammation and subsequent injury by inhibiting activation of the airway epithelium...
- Discordant extracellular superoxide dismutase expression and activity in neonatal hyperoxic lungLisa B Mamo
Department of Pediatrics, Duke University Medical Center, Durham, North Carolina, USA
Am J Respir Crit Care Med 170:313-8. 2004..These data provide the first direct evidence that EC-SOD is itself a target of nitration in hyperoxia, and offer a plausible explanation for low EC-SOD activity despite its increased secretion by O(2)-exposed neonatal lung...
- The role of Scgb1a1+ Clara cells in the long-term maintenance and repair of lung airway, but not alveolar, epitheliumEmma L Rawlins
Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
Cell Stem Cell 4:525-34. 2009..Rather, our results support a model in which the trachea, bronchioles, and alveoli are maintained by distinct populations of epithelial progenitor cells...
- Mast cells mediate hyperoxia-induced airway hyper-reactivity in newborn ratsEric D Schultz
Department of Pediatrics, Duke University Medical Center, Durham, NC 27710, USA
Pediatr Res 68:70-4. 2010..Imatinib treatment completely blocked mast cell accumulation in tracheal/hilar airway tissues. Hyperoxia-induced AHR in neonatal rats is mediated, at least in part, via the mast cell...
- Pulmonary alveolar epithelial uptake of S-nitrosothiols is regulated by L-type amino acid transporterOlivia M Granillo
School of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
Am J Physiol Lung Cell Mol Physiol 295:L38-43. 2008..This was unaffected by PEPT2 inhibition. We conclude that RSNOs (thionitrites, S-nitrosothiols) and NO enter alveolar epithelial cells predominantly by S-nitrosation of L-Cys, which is then imported through LAT...
- CXCR2 blockade reduces radical formation in hyperoxia-exposed newborn rat lungLingjie Liao
Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China
Pediatr Res 60:299-303. 2006..Taken together with our previous studies, the present findings show that blocking neutrophil influx protects alveolar development and improves lung function in part by preventing reactive oxygen/nitrogen species accumulation...
- Early inhaled nitric oxide therapy in premature newborns with respiratory failureJohn P Kinsella
Pediatric Heart Lung Center, University of Colorado School of Medicine, and Children s Hospital, Denver, USA
N Engl J Med 355:354-64. 2006..The safety and efficacy of early, low-dose, prolonged therapy with inhaled nitric oxide in premature newborns with respiratory failure are uncertain...
- Opposing effects of 60% oxygen and neutrophil influx on alveologenesis in the neonatal ratMan Yi
Lung Biology Programme, The Hospital for Sick Children, 555 University Avenue, Toronto, ON M5G 1X8, Canada
Am J Respir Crit Care Med 170:1188-96. 2004..These data suggest that exposure of the neonatal lung to moderate hyperoxia may enhance postnatal lung growth, provided postnatal pulmonary inflammation is suppressed...
- Chemokine Blockade to Preserve Lung DevelopmentRichard Auten; Fiscal Year: 2005..Aim 3 will determine whether blockade of leukocyte function can safely preserve normal alveolar development during recovery from severe oxidant stress. ..