S Alam

Summary

Affiliation: Duke University Medical Center
Country: USA

Publications

  1. pmc Induction of antibodies in rhesus macaques that recognize a fusion-intermediate conformation of HIV-1 gp41
    S Moses Dennison
    Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina, United States of America
    PLoS ONE 6:e27824. 2011
  2. pmc Antigenicity and immunogenicity of RV144 vaccine AIDSVAX clade E envelope immunogen is enhanced by a gp120 N-terminal deletion
    S Munir Alam
    Duke Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina, USA
    J Virol 87:1554-68. 2013
  3. pmc Differential reactivity of germ line allelic variants of a broadly neutralizing HIV-1 antibody to a gp41 fusion intermediate conformation
    S Munir Alam
    Human Vaccine Institute, Duke University Medical Center, 2 Genome Court, P O Box 103020, Durham, NC 27710, USA
    J Virol 85:11725-31. 2011
  4. pmc Role of HIV membrane in neutralization by two broadly neutralizing antibodies
    S Munir Alam
    Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 106:20234-9. 2009
  5. pmc Human immunodeficiency virus type 1 gp41 antibodies that mask membrane proximal region epitopes: antibody binding kinetics, induction, and potential for regulation in acute infection
    S Munir Alam
    Human Vaccine Institute, Box 3258, Duke University Medical Center, MSRBII Bldg, Room 4042, Durham, NC 27710, USA
    J Virol 82:115-25. 2008
  6. pmc The role of antibody polyspecificity and lipid reactivity in binding of broadly neutralizing anti-HIV-1 envelope human monoclonal antibodies 2F5 and 4E10 to glycoprotein 41 membrane proximal envelope epitopes
    S Munir Alam
    Department of Medicine, Duke Human Vaccine Institute, Duke University School of Medicine, RP1 Circuit Drive, Durham, NC 27710, USA
    J Immunol 178:4424-35. 2007
  7. pmc Stable docking of neutralizing human immunodeficiency virus type 1 gp41 membrane-proximal external region monoclonal antibodies 2F5 and 4E10 is dependent on the membrane immersion depth of their epitope regions
    S Moses Dennison
    Human Vaccine Institute, Department of Medicine, Duke University School of Medicine, Durham, North Carolina 27710, USA
    J Virol 83:10211-23. 2009
  8. pmc Prolonged exposure of the HIV-1 gp41 membrane proximal region with L669S substitution
    Xiaoying Shen
    Department of Surgery, Duke Human Vaccine Institute, Duke University, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 107:5972-7. 2010
  9. ncbi request reprint An inducible HIV type 1 gp41 HR-2 peptide-binding site on HIV type 1 envelope gp120
    S Munir Alam
    Department of Medicine, Duke Center for AIDS Research, Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27707, USA
    AIDS Res Hum Retroviruses 20:836-45. 2004
  10. ncbi request reprint Cardiolipin polyspecific autoreactivity in two broadly neutralizing HIV-1 antibodies
    Barton F Haynes
    Duke University School of Medicine, Durham, NC 27710, USA
    Science 308:1906-8. 2005

Research Grants

Collaborators

Detail Information

Publications30

  1. pmc Induction of antibodies in rhesus macaques that recognize a fusion-intermediate conformation of HIV-1 gp41
    S Moses Dennison
    Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina, United States of America
    PLoS ONE 6:e27824. 2011
    ..Nonetheless, the Env-liposome prime-boost immunization strategy induced antibodies that recognized a gp41 fusion intermediate protein and was successful in focusing the antibody response to the desired epitope...
  2. pmc Antigenicity and immunogenicity of RV144 vaccine AIDSVAX clade E envelope immunogen is enhanced by a gp120 N-terminal deletion
    S Munir Alam
    Duke Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina, USA
    J Virol 87:1554-68. 2013
    ..These results demonstrate that deletion of N-terminal residues in the RV144 A244 gp120 immunogen improves both envelope antigenicity and immunogenicity...
  3. pmc Differential reactivity of germ line allelic variants of a broadly neutralizing HIV-1 antibody to a gp41 fusion intermediate conformation
    S Munir Alam
    Human Vaccine Institute, Duke University Medical Center, 2 Genome Court, P O Box 103020, Durham, NC 27710, USA
    J Virol 85:11725-31. 2011
    ..Thus, these data demonstrate a genetically determined trait that may affect host responses to HIV-1 envelope epitopes recognized by broadly neutralizing antibodies and has implications for unmutated ancestor-based immunogen design...
  4. pmc Role of HIV membrane in neutralization by two broadly neutralizing antibodies
    S Munir Alam
    Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 106:20234-9. 2009
    ..These results bear directly on strategies for rational design of HIV-1 envelope immunogens...
  5. pmc Human immunodeficiency virus type 1 gp41 antibodies that mask membrane proximal region epitopes: antibody binding kinetics, induction, and potential for regulation in acute infection
    S Munir Alam
    Human Vaccine Institute, Box 3258, Duke University Medical Center, MSRBII Bldg, Room 4042, Durham, NC 27710, USA
    J Virol 82:115-25. 2008
    ....
  6. pmc The role of antibody polyspecificity and lipid reactivity in binding of broadly neutralizing anti-HIV-1 envelope human monoclonal antibodies 2F5 and 4E10 to glycoprotein 41 membrane proximal envelope epitopes
    S Munir Alam
    Department of Medicine, Duke Human Vaccine Institute, Duke University School of Medicine, RP1 Circuit Drive, Durham, NC 27710, USA
    J Immunol 178:4424-35. 2007
    ..Taken together, these data demonstrate the similarity of 2F5 and 4E10 mAbs to known anti-cardiolipin Abs and support the model that mAb 2F5 and 4E10 binding to HIV-1 involves both viral lipid membrane and gp41 membrane proximal epitopes...
  7. pmc Stable docking of neutralizing human immunodeficiency virus type 1 gp41 membrane-proximal external region monoclonal antibodies 2F5 and 4E10 is dependent on the membrane immersion depth of their epitope regions
    S Moses Dennison
    Human Vaccine Institute, Department of Medicine, Duke University School of Medicine, Durham, North Carolina 27710, USA
    J Virol 83:10211-23. 2009
    ..These data have important implications for the design and use of peptide-liposome conjugates as immunogens for the induction of MPER-neutralizing antibodies...
  8. pmc Prolonged exposure of the HIV-1 gp41 membrane proximal region with L669S substitution
    Xiaoying Shen
    Department of Surgery, Duke Human Vaccine Institute, Duke University, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 107:5972-7. 2010
    ..These data suggest that a major contribution to the L669S mutant virus phenotype of enhanced susceptibility to MPER mAbs is prolonged exposure of the MPER neutralizing epitope during viral entry...
  9. ncbi request reprint An inducible HIV type 1 gp41 HR-2 peptide-binding site on HIV type 1 envelope gp120
    S Munir Alam
    Department of Medicine, Duke Center for AIDS Research, Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27707, USA
    AIDS Res Hum Retroviruses 20:836-45. 2004
    ..This may prove to be an important consideration when designing an HIV vaccine that utilizes constrained HIV Env proteins...
  10. ncbi request reprint Cardiolipin polyspecific autoreactivity in two broadly neutralizing HIV-1 antibodies
    Barton F Haynes
    Duke University School of Medicine, Durham, NC 27710, USA
    Science 308:1906-8. 2005
    ..These results may have important implications for generating effective neutralizing antibody responses by using HIV-1 vaccines...
  11. pmc Anti-phospholipid human monoclonal antibodies inhibit CCR5-tropic HIV-1 and induce beta-chemokines
    M Anthony Moody
    Duke Human Vaccine Institute, Duke University Medical Center, Durham, NC 27710, USA
    J Exp Med 207:763-76. 2010
    ..The release of these beta-chemokines explains both the specificity for R5 HIV-1 and the activity of these mAbs in PBMC cultures containing both primary lymphocytes and monocytes...
  12. pmc Antigenicity and immunogenicity of a synthetic human immunodeficiency virus type 1 group m consensus envelope glycoprotein
    Feng Gao
    Department of Medicine, Duke University Medical Center, 112 Research Park III, Research Dr, Box 3347, Durham, NC 27710, USA
    J Virol 79:1154-63. 2005
    ..Thus, the computer-generated "consensus" env genes are capable of expressing envelope glycoproteins that retain the structural, functional, and immunogenic properties of wild-type HIV-1 envelopes...
  13. pmc Autoreactivity in an HIV-1 broadly reactive neutralizing antibody variable region heavy chain induces immunologic tolerance
    Laurent Verkoczy
    Duke Human Vaccine Institute and Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 107:181-6. 2010
    ..These features are consistent with elimination of 2F5 HC autoreactivity by additional negative selection mechanism(s) in the periphery...
  14. pmc Functional, non-clonal IgMa-restricted B cell receptor interactions with the HIV-1 envelope gp41 membrane proximal external region
    Laurent Verkoczy
    Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina, United States of America
    PLoS ONE 4:e7215. 2009
    ..These data suggest that low avidity, non-paratopic interactions between the gp41 MPER and membrane Ig on naïve B cells may interfere with or divert bnAb responses...
  15. pmc Cross-reactive monoclonal antibodies to multiple HIV-1 subtype and SIVcpz envelope glycoproteins
    Feng Gao
    Duke Human Vaccine Institute, Duke University Medical Center, Durham, NC 27710, USA
    Virology 394:91-8. 2009
    ..Nonetheless, such mAbs represent valuable reagents to study the biochemistry and structural biology of Env protein oligomers...
  16. pmc HIV-1 hides an Achilles' heel in virion lipids
    Barton F Haynes
    Duke Human Vaccine Institute, Departments of Medicine, Pathology, and Immunology, Duke University School of Medicine, Durham, NC 27710, USA
    Immunity 28:10-2. 2008
    ..In this issue, Sun et al. (2008) model the interface between a neutralizing antibody and HIV-1 glycoprotein 41 (gp41) envelope peptide in lipids. This model could help overcome a roadblock in HIV-1 vaccine development...
  17. ncbi request reprint Clustered charged amino acids of human adenosine deaminase comprise a functional epitope for binding the adenosine deaminase complexing protein CD26/dipeptidyl peptidase IV
    Eva Richard
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 277:19720-6. 2002
    ..1-1.3 kcal/mol. This cluster of 3 charged residues appears to be a "functional epitope" that accounts for about half of the difference between human and mouse ADA in free energy of binding to CD26...
  18. pmc Antibody polyspecificity and neutralization of HIV-1: a hypothesis
    Barton F Haynes
    Duke Human Vaccine Institute, Duke University School of Medicine, Durham NC 27710, USA
    Hum Antibodies 14:59-67. 2005
    ....
  19. ncbi request reprint Detection of Ebola virus envelope using monoclonal and polyclonal antibodies in ELISA, surface plasmon resonance and a quartz crystal microbalance immunosensor
    Jae Sung Yu
    Human Vaccine Institute, Duke University Medical Center, Durham, NC 27710, United States
    J Virol Methods 137:219-28. 2006
    ..Thus, polyclonal and monoclonal antibodies can be used in combination to identify and differentiate both human and non-human primate EBOV GPs...
  20. pmc A group M consensus envelope glycoprotein induces antibodies that neutralize subsets of subtype B and C HIV-1 primary viruses
    Hua Xin Liao
    Duke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA
    Virology 353:268-82. 2006
    ..Our study also shows the feasibility of iterative improvements in Env immunogenicity by rational design of centralized genes...
  21. pmc Immunogenicity of constrained monoclonal antibody A32-human immunodeficiency virus (HIV) Env gp120 complexes compared to that of recombinant HIV type 1 gp120 envelope glycoproteins
    Hua Xin Liao
    Duke Human Vaccine Institute and Department of Medicine, Duke University School of Medicine, Durham, NC 27710, USA
    J Virol 78:5270-8. 2004
    ....
  22. pmc In vitro and in vivo characterization of anthrax anti-protective antigen and anti-lethal factor monoclonal antibodies after passive transfer in a mouse lethal toxin challenge model to define correlates of immunity
    Herman F Staats
    Department of Pathology, Box 3712, DUMC, Durham, NC 27710, USA
    Infect Immun 75:5443-52. 2007
    ..Thus, this LeTx neutralization assay may be a more biologically relevant neutralization assay to predict the in vivo protective capacity of LeTx-neutralizing antibodies...
  23. doi request reprint Crystal structure of a non-neutralizing antibody to the HIV-1 gp41 membrane-proximal external region
    Nathan I Nicely
    Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina, USA
    Nat Struct Mol Biol 17:1492-4. 2010
    ..We show that unlike 2F5, 13H11 binds to a well-defined helical MPER structure that is consistent with the structure of gp41 in a post-fusion six-helix bundle conformation...
  24. ncbi request reprint Expression of the CD7 ligand K-12 in human thymic epithelial cells: regulation by IFN-gamma
    Gordon K Lam
    Department of Medicine, Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina, USA
    J Clin Immunol 25:41-9. 2005
    ..These data suggest a role for thymic microenvironment-produced K12 in regulation of thymocyte signaling and cytokine release, particularly in the setting of thymus pathology where IFN-gamma is upregulated such as myasthenia gravis...
  25. ncbi request reprint CX3CR1 tyrosine sulfation enhances fractalkine-induced cell adhesion
    Alan M Fong
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 277:19418-23. 2002
    ..Further, tyrosine sulfation may represent a general mechanism utilized by molecules that function in the rapid capture of circulating leukocytes...
  26. ncbi request reprint Binding kinetics of superantigen with TCR and MHC class II
    S Munir Alam
    Division of Rheumatology, Allergy and Clinical Immunology, Department of Medicine, Duke University Medical Center, Durham, NC, USA
    Methods Mol Biol 214:65-85. 2003
  27. pmc Nonneutralizing HIV-1 gp41 envelope cluster II human monoclonal antibodies show polyreactivity for binding to phospholipids and protein autoantigens
    S Moses Dennison
    Duke Human Vaccine Institute and Department of Medicine, Duke University School of Medicine, Durham, North Carolina 27710, USA
    J Virol 85:1340-7. 2011
    ..These results demonstrate that lipid-reactive gp41 cluster II antibodies are nonneutralizing due to their inability to bind to the relevant neutralizing epitopes on gp41...
  28. pmc C-terminal repeat domain kinase I phosphorylates Ser2 and Ser5 of RNA polymerase II C-terminal domain repeats
    Janice C Jones
    Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
    J Biol Chem 279:24957-64. 2004
    ..We also observed that CTDK-I efficiently generates doubly phosphorylated CTD repeats; CTD substrates that already contain Ser2-PO(4) or Ser5-PO(4) are more readily phosphorylated CTDK-I than unphosphorylby ated CTD substrates...
  29. ncbi request reprint Allelic exclusion of the TCR alpha-chain is an active process requiring TCR-mediated signaling and c-Cbl
    Nathalie Niederberger
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Immunol 170:4557-63. 2003
    ..Thus, not only is there an important role for TCR signaling in causing alpha-chain allelic exclusion, but differential ubiquitination by c-Cbl may be an important factor in causing only the nonselected alpha-chain to be down-modulated...
  30. pmc Glycosylation site-specific analysis of HIV envelope proteins (JR-FL and CON-S) reveals major differences in glycosylation site occupancy, glycoform profiles, and antigenic epitopes' accessibility
    Eden P Go
    Department of Chemistry, University of Kansas, Lawrence, Kansas 66045, USA
    J Proteome Res 7:1660-74. 2008
    ....

Research Grants3

  1. Coreceptor Modulation of TCR-MHC Interactions
    S Alam; Fiscal Year: 2003
    ..abstract_text> ..