Alexander Mazin

Summary

Affiliation: Drexel University College of Medicine
Country: USA

Publications

  1. ncbi Human Rad54 protein stimulates DNA strand exchange activity of hRad51 protein in the presence of Ca2+
    Olga M Mazina
    Department of Biochemistry, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102 1192, USA
    J Biol Chem 279:52042-51. 2004
  2. ncbi Rad54 protein promotes branch migration of Holliday junctions
    Dmitry V Bugreev
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102 1192, USA
    Nature 442:590-3. 2006
  3. ncbi Rad51 protein stimulates the branch migration activity of Rad54 protein
    Matthew J Rossi
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102 1192, USA
    J Biol Chem 283:24698-706. 2008
  4. ncbi Rad54, the motor of homologous recombination
    Alexander V Mazin
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102, USA
    DNA Repair (Amst) 9:286-302. 2010
  5. ncbi A novel function of Rad54 protein. Stabilization of the Rad51 nucleoprotein filament
    Alexander V Mazin
    Department of Biochemistry, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102 1192, USA
    J Biol Chem 278:14029-36. 2003
  6. ncbi Bloom syndrome helicase stimulates RAD51 DNA strand exchange activity through a novel mechanism
    Dmitry V Bugreev
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102 1192, USA
    J Biol Chem 284:26349-59. 2009
  7. ncbi Novel pro- and anti-recombination activities of the Bloom's syndrome helicase
    Dmitry V Bugreev
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102, USA
    Genes Dev 21:3085-94. 2007
  8. ncbi Rad54 dissociates homologous recombination intermediates by branch migration
    Dmitry V Bugreev
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102 1192, USA
    Nat Struct Mol Biol 14:746-53. 2007
  9. ncbi Interactions of human rad54 protein with branched DNA molecules
    Olga M Mazina
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102 1192, USA
    J Biol Chem 282:21068-80. 2007
  10. ncbi Human Rad54 protein stimulates human Mus81-Eme1 endonuclease
    Olga M Mazina
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102 1192, USA
    Proc Natl Acad Sci U S A 105:18249-54. 2008

Research Grants

  1. Repair of DNA breaks in human: the role of Rad54 protein
    Alexander Mazin; Fiscal Year: 2007
  2. Repair of DNA breaks in humans: the role of Rad54 protein
    Alexander V Mazin; Fiscal Year: 2010

Collaborators

  • R M Brosh
  • E Egelman
  • Dmitry V Bugreev
  • Olga M Mazina
  • Matthew J Rossi
  • J S Deakyne
  • Xiong Yu
  • Fumio Hanaoka
  • Nicolas H Thomaä
  • Andrzej Stasiak
  • Alicja Z Stasiak
  • Efim I Golub

Detail Information

Publications15

  1. ncbi Human Rad54 protein stimulates DNA strand exchange activity of hRad51 protein in the presence of Ca2+
    Olga M Mazina
    Department of Biochemistry, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102 1192, USA
    J Biol Chem 279:52042-51. 2004
    ..The results obtained indicate that hRad54 protein serves as a dsDNA gateway for the hRad51-ssDNA filament, promoting binding and an ATP hydrolysis-dependent translocation of dsDNA during the search for homologous sequences...
  2. ncbi Rad54 protein promotes branch migration of Holliday junctions
    Dmitry V Bugreev
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102 1192, USA
    Nature 442:590-3. 2006
    ..13). Novel DNA branch-migration activity is fully consistent with this late homologous recombination function of Rad54 protein...
  3. ncbi Rad51 protein stimulates the branch migration activity of Rad54 protein
    Matthew J Rossi
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102 1192, USA
    J Biol Chem 283:24698-706. 2008
    ..The current results support the hypothesis that the hRad51 conformation state has a strong effect on interaction with hRad54 and ultimately on the function of hRad54 in homologous recombination...
  4. ncbi Rad54, the motor of homologous recombination
    Alexander V Mazin
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102, USA
    DNA Repair (Amst) 9:286-302. 2010
    ..The current review focuses on the recently identified Rad54 activities which contribute to the late phase of HR, especially the branch migration of Holliday junctions...
  5. ncbi A novel function of Rad54 protein. Stabilization of the Rad51 nucleoprotein filament
    Alexander V Mazin
    Department of Biochemistry, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102 1192, USA
    J Biol Chem 278:14029-36. 2003
    ..Thus, the co-assembly of an interacting partner with the Rad51 nucleoprotein filament represents a novel means of stabilizing the biochemical entity central to homologous recombination, and reveals a new function of Rad54 protein...
  6. ncbi Bloom syndrome helicase stimulates RAD51 DNA strand exchange activity through a novel mechanism
    Dmitry V Bugreev
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102 1192, USA
    J Biol Chem 284:26349-59. 2009
    ..These data suggest a novel BLM function that is stimulation of the RAD51 DNA pairing. Our results demonstrate the important role of the RAD51 nucleoprotein filament conformation in stimulation of DNA pairing by BLM...
  7. ncbi Novel pro- and anti-recombination activities of the Bloom's syndrome helicase
    Dmitry V Bugreev
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102, USA
    Genes Dev 21:3085-94. 2007
    ..Using in vitro reconstitution reactions, we analyzed how different biochemical activities of BLM contribute to its functions in homologous recombination...
  8. ncbi Rad54 dissociates homologous recombination intermediates by branch migration
    Dmitry V Bugreev
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102 1192, USA
    Nat Struct Mol Biol 14:746-53. 2007
    ..These results suggest a previously uncharacterized mechanism of DSB repair in which Rad54 branch-migration activity plays an important role...
  9. ncbi Interactions of human rad54 protein with branched DNA molecules
    Olga M Mazina
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102 1192, USA
    J Biol Chem 282:21068-80. 2007
    ..This pleomorphism of hRad54 in formation of oligomeric complexes with DNA may correspond to multiple functions of the protein in homologous recombination...
  10. ncbi Human Rad54 protein stimulates human Mus81-Eme1 endonuclease
    Olga M Mazina
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102 1192, USA
    Proc Natl Acad Sci U S A 105:18249-54. 2008
    ....
  11. ncbi Fanconi anemia: at the crossroads of DNA repair
    J S Deakyne
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102, USA
    Biochemistry (Mosc) 76:36-48. 2011
    ..The goal is to clarify how the proteins work together to carry out interstrand crosslink repair and homologous recombination-mediated repair of damaged DNA...
  12. ncbi RECQ1 possesses DNA branch migration activity
    Dmitry V Bugreev
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102 1192, USA
    J Biol Chem 283:20231-42. 2008
    ..The newly found enzymatic properties of the RECQ1 helicase may have important implications for the function of RECQ1 in maintenance of genomic stability...
  13. ncbi Activation of human meiosis-specific recombinase Dmc1 by Ca2+
    Dmitry V Bugreev
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102, USA
    J Biol Chem 280:26886-95. 2005
    ..single-stranded DNA complexes. Our results demonstrate a universal role of Ca2+ in stimulation of mammalian DNA strand exchange proteins and reveal diversity in the mechanisms of this stimulation...
  14. ncbi Ca2+ activates human homologous recombination protein Rad51 by modulating its ATPase activity
    Dmitry V Bugreev
    Department of Biochemistry, Drexel University College of Medicine, Philadelphia, PA 19102 1192, USA
    Proc Natl Acad Sci U S A 101:9988-93. 2004
    ..This mechanism of Rad51 protein regulation by modulating its ATPase activity is evolutionarily recent; we found no such mechanism for yeast Rad51 (yRad51) protein...
  15. ncbi Analyzing the branch migration activities of eukaryotic proteins
    Matthew J Rossi
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102 1192, USA
    Methods 51:336-46. 2010
    ..Here, we describe methods employing a wide range of DNA substrates for studying proteins that catalyze branch migration of Holliday junctions...

Research Grants9

  1. Repair of DNA breaks in human: the role of Rad54 protein
    Alexander Mazin; Fiscal Year: 2007
    ..The knowledge acquired in this study will help to better understand the fundamental mechanisms of HR and will be applied for developing efficient tumor therapies. ..
  2. Repair of DNA breaks in humans: the role of Rad54 protein
    Alexander V Mazin; Fiscal Year: 2010
    ..Knowledge of the mechanisms of homologous recombination leads to more efficient approaches for cancer treatment. ..