Thomas Edlind

Summary

Affiliation: Drexel University College of Medicine
Country: USA

Publications

  1. ncbi request reprint Antifungal activity in Saccharomyces cerevisiae is modulated by calcium signalling
    Thomas Edlind
    Department of Microbiology and Immunology, MCP Hahnemann School of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USA
    Mol Microbiol 46:257-68. 2002
  2. ncbi request reprint Promoter-dependent disruption of genes: simple, rapid, and specific PCR-based method with application to three different yeast
    Thomas D Edlind
    Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 19129, USA
    Curr Genet 48:117-25. 2005
  3. pmc Mutational analysis of flucytosine resistance in Candida glabrata
    Thomas D Edlind
    Department of Microbiology and Immunology, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USA
    Antimicrob Agents Chemother 54:4733-8. 2010
  4. pmc Flucytosine antagonism of azole activity versus Candida glabrata: role of transcription factor Pdr1 and multidrug transporter Cdr1
    Zoë Steier
    Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania, USA
    Antimicrob Agents Chemother 57:5543-7. 2013
  5. pmc Cyclic AMP signaling pathway modulates susceptibility of candida species and Saccharomyces cerevisiae to antifungal azoles and other sterol biosynthesis inhibitors
    Pooja Jain
    Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129, USA
    Antimicrob Agents Chemother 47:3195-201. 2003
  6. pmc Candida albicans and Candida glabrata clinical isolates exhibiting reduced echinocandin susceptibility
    Santosh Katiyar
    Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 19129, USA
    Antimicrob Agents Chemother 50:2892-4. 2006
  7. pmc Azole resistance in Candida glabrata: coordinate upregulation of multidrug transporters and evidence for a Pdr1-like transcription factor
    John Paul Vermitsky
    Department of Microbiology and Immunology, Drexel University College of Medicine, 2900 Queen Ln, Philadelphia, PA 19129, USA
    Antimicrob Agents Chemother 48:3773-81. 2004
  8. pmc Role for Fks1 in the intrinsic echinocandin resistance of Fusarium solani as evidenced by hybrid expression in Saccharomyces cerevisiae
    Santosh K Katiyar
    Department of Microbiology and Immunology, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USA
    Antimicrob Agents Chemother 53:1772-8. 2009
  9. pmc Candida glabrata mutants demonstrating paradoxical reduced caspofungin susceptibility but increased micafungin susceptibility
    Kelley R Healey
    Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 19129, USA
    Antimicrob Agents Chemother 55:3947-9. 2011
  10. pmc New Fks hot spot for acquired echinocandin resistance in Saccharomyces cerevisiae and its contribution to intrinsic resistance of Scedosporium species
    Michael E Johnson
    Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 19129 1033, USA
    Antimicrob Agents Chemother 55:3774-81. 2011

Research Grants

  1. ANTIFUNGAL RESPONSE--MDR INDUCTION
    Thomas Edlind; Fiscal Year: 1999
  2. Candida glabrata Pdr1: Master Regulator of Azole Resistance
    Thomas D Edlind; Fiscal Year: 2010
  3. Mutational Analysis of Fks1: Intrinsic Echinocandin Resistance
    Thomas Edlind; Fiscal Year: 2009
  4. Candida glabrata Pdr1: Master Regulator of Azole Resistance
    Thomas Edlind; Fiscal Year: 2009
  5. Signaling Pathways in Yeast Azole Response
    Thomas Edlind; Fiscal Year: 2005
  6. Signaling Pathways in Yeast Azole Response
    Thomas Edlind; Fiscal Year: 2003
  7. Signaling Pathways in Yeast Azole Response
    Thomas Edlind; Fiscal Year: 2004
  8. Signaling Pathways in Yeast Azole Response
    Thomas Edlind; Fiscal Year: 2003
  9. Signaling Pathways in Yeast Azole Response
    Thomas Edlind; Fiscal Year: 2002
  10. ANTIFUNGAL RESPONSE--MDR INDUCTION
    Thomas Edlind; Fiscal Year: 2002

Collaborators

Detail Information

Publications17

  1. ncbi request reprint Antifungal activity in Saccharomyces cerevisiae is modulated by calcium signalling
    Thomas Edlind
    Department of Microbiology and Immunology, MCP Hahnemann School of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USA
    Mol Microbiol 46:257-68. 2002
    ..cerevisiae with azole or terbinafine resulted in transcriptional upregulation of genes FKS2 and PMR1 known to be Ca2+ regulated. A model to explain the role of Ca2+-regulated signalling in azole/terbinafine tolerance is proposed...
  2. ncbi request reprint Promoter-dependent disruption of genes: simple, rapid, and specific PCR-based method with application to three different yeast
    Thomas D Edlind
    Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 19129, USA
    Curr Genet 48:117-25. 2005
    ..Using this PDR5 promoter-URA3 recombinant, we further demonstrate a simple extension of the method that yields regulatory mutants via 5-fluoroorotic acid selection. PRODIGE warrants testing in other yeast, molds, and beyond...
  3. pmc Mutational analysis of flucytosine resistance in Candida glabrata
    Thomas D Edlind
    Department of Microbiology and Immunology, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USA
    Antimicrob Agents Chemother 54:4733-8. 2010
    ..These data provide a framework for understanding 5FC resistance in C. glabrata and potentially in other fungal pathogens...
  4. pmc Flucytosine antagonism of azole activity versus Candida glabrata: role of transcription factor Pdr1 and multidrug transporter Cdr1
    Zoë Steier
    Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania, USA
    Antimicrob Agents Chemother 57:5543-7. 2013
    ..Thus, mitochondrial dysfunction resulting in Pdr1 activation is the likely basis for 5FC antagonism of azole activity versus C. glabrata. ..
  5. pmc Cyclic AMP signaling pathway modulates susceptibility of candida species and Saccharomyces cerevisiae to antifungal azoles and other sterol biosynthesis inhibitors
    Pooja Jain
    Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129, USA
    Antimicrob Agents Chemother 47:3195-201. 2003
    ..Our results implicate cAMP signaling in the yeast azole response; compounds similar to MDL-12330A may be useful adjuvants in azole therapy...
  6. pmc Candida albicans and Candida glabrata clinical isolates exhibiting reduced echinocandin susceptibility
    Santosh Katiyar
    Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 19129, USA
    Antimicrob Agents Chemother 50:2892-4. 2006
    ..glabrata confirmed its role in RES. Y641-equivalent Fks residues were identified in intrinsically RES Fusarium species and Candida guilliermondii...
  7. pmc Azole resistance in Candida glabrata: coordinate upregulation of multidrug transporters and evidence for a Pdr1-like transcription factor
    John Paul Vermitsky
    Department of Microbiology and Immunology, Drexel University College of Medicine, 2900 Queen Ln, Philadelphia, PA 19129, USA
    Antimicrob Agents Chemother 48:3773-81. 2004
    ..Additional regulatory factors remain to be identified, as indicated by the lack of PDR1 mutation in a clinical isolate with coordinately upregulated CDR1-PDH1...
  8. pmc Role for Fks1 in the intrinsic echinocandin resistance of Fusarium solani as evidenced by hybrid expression in Saccharomyces cerevisiae
    Santosh K Katiyar
    Department of Microbiology and Immunology, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USA
    Antimicrob Agents Chemother 53:1772-8. 2009
    ..cerevisiae and 8- to 32-fold less than that of the same hybrid with an F639 mutation. We propose that Fks1 sequences represent determinants of intrinsic EC resistance in Fusarium and Scedosporium species and, potentially, other fungi...
  9. pmc Candida glabrata mutants demonstrating paradoxical reduced caspofungin susceptibility but increased micafungin susceptibility
    Kelley R Healey
    Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 19129, USA
    Antimicrob Agents Chemother 55:3947-9. 2011
    ..CRS-MIS mutants were generated from all 10 C. glabrata strains tested and were tentatively identified within a collection of clinical isolates. Intriguingly, sequencing and gene disruption demonstrated that CRS-MIS is Fks independent...
  10. pmc New Fks hot spot for acquired echinocandin resistance in Saccharomyces cerevisiae and its contribution to intrinsic resistance of Scedosporium species
    Michael E Johnson
    Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 19129 1033, USA
    Antimicrob Agents Chemother 55:3774-81. 2011
    ..Fks1 sequences from intrinsically RES Scedosporium species revealed W695F-equivalent substitutions; Fks1 hybrids expressing Scedosporium prolificans hot spot 3 confirmed that this substitution imparts RES...
  11. pmc Effects of cetylpyridinium chloride resistance and treatment on fluconazole activity versus Candida albicans
    Merritt P Edlind
    Department of Microbiology and Immunology, Drexel University of College of Medicine, 2900 Queen Ln, Philadelphia, PA 19129, USA
    Antimicrob Agents Chemother 49:843-5. 2005
    ..MICs were unchanged, and trailing growth generally decreased. With CPC-fluconazole combinations, both antagonism and synergism were observed, which can be explained, in part, by CDR1-CDR2 multidrug transporter upregulation...
  12. ncbi request reprint Pdr1 regulates multidrug resistance in Candida glabrata: gene disruption and genome-wide expression studies
    John Paul Vermitsky
    Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA, USA
    Mol Microbiol 61:704-22. 2006
    ..C. glabrata provides a promising model for studying the genetic basis of multidrug resistance and its impact on virulence...
  13. pmc ROX1 and ERG regulation in Saccharomyces cerevisiae: implications for antifungal susceptibility
    Karl W Henry
    Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129, USA
    Eukaryot Cell 1:1041-4. 2002
    ..These inverse correlations suggest that transcriptional regulation of ROX1 is an important determinant of ERG expression and hence of azole and terbinafine susceptibilities...
  14. pmc Histone deacetylase inhibitors enhance Candida albicans sensitivity to azoles and related antifungals: correlation with reduction in CDR and ERG upregulation
    W Lamar Smith
    Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129, USA
    Antimicrob Agents Chemother 46:3532-9. 2002
    ..This most likely explains the inhibition of azole and terbinafine trailing by TSA and, more generally, provides evidence that trailing is mediated by upregulation of target enzymes and multidrug transporters...
  15. pmc Increases in SLT2 expression and chitin content are associated with incomplete killing of Candida glabrata by caspofungin
    Jason M Cota
    University of Texas at Austin College of Pharmacy, UTHSCSA, PERC MSC 6220, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA
    Antimicrob Agents Chemother 52:1144-6. 2008
    ..In contrast, fungicidal activity and no chitin increase were observed in an isogenic Delta slt2 strain, suggesting a role for SLT2 and chitin production in the response of C. glabrata to caspofungin...
  16. pmc The echinocandin "target" identified by cross-linking is a homolog of Pil1 and Lsp1, sphingolipid-dependent regulators of cell wall integrity signaling
    Thomas D Edlind
    Antimicrob Agents Chemother 48:4491. 2004
  17. ncbi request reprint Proteomic analysis of experimentally induced azole resistance in Candida glabrata
    P David Rogers
    Department of Pharmacy, College of Pharmacy, University of Tennessee Health Science Center Memphis, TN 38163, USA
    J Antimicrob Chemother 58:434-8. 2006
    ....

Research Grants15

  1. ANTIFUNGAL RESPONSE--MDR INDUCTION
    Thomas Edlind; Fiscal Year: 1999
    ..Candida homologues of these factors will be identified by PCR or expression in S. cerevisiae. Their role in MDR induction will be examined by gene disruption. ..
  2. Candida glabrata Pdr1: Master Regulator of Azole Resistance
    Thomas D Edlind; Fiscal Year: 2010
    ..glabrata Pdr1, "master regulator" of azole resistance;they will confer a deep understanding of the molecular basis for azole resistance, and could lead to novel strategies to prevent or reverse resistance. ..
  3. Mutational Analysis of Fks1: Intrinsic Echinocandin Resistance
    Thomas Edlind; Fiscal Year: 2009
    ..These studies will accelerate the development of second generation ECs that provide a broader spectrum of protection from fungal pathogens. ..
  4. Candida glabrata Pdr1: Master Regulator of Azole Resistance
    Thomas Edlind; Fiscal Year: 2009
    ..glabrata Pdr1, "master regulator" of azole resistance; they will confer a deep understanding of the molecular basis for azole resistance, and could lead to novel strategies to prevent or reverse resistance. ..
  5. Signaling Pathways in Yeast Azole Response
    Thomas Edlind; Fiscal Year: 2005
    ..Finally, the signalling pathway responsible for azole-dependent upregulation of ERG expression will be elucidated with a focus on Rox1p and Haplp transcription factors. ..
  6. Signaling Pathways in Yeast Azole Response
    Thomas Edlind; Fiscal Year: 2003
    ..Finally, the signalling pathway responsible for azole-dependent upregulation of ERG expression will be elucidated with a focus on Rox1p and Haplp transcription factors. ..
  7. Signaling Pathways in Yeast Azole Response
    Thomas Edlind; Fiscal Year: 2004
    ..Finally, the signalling pathway responsible for azole-dependent upregulation of ERG expression will be elucidated with a focus on Rox1p and Haplp transcription factors. ..
  8. Signaling Pathways in Yeast Azole Response
    Thomas Edlind; Fiscal Year: 2003
    ..Finally, the signalling pathway responsible for azole-dependent upregulation of ERG expression will be elucidated with a focus on Rox1p and Haplp transcription factors. ..
  9. Signaling Pathways in Yeast Azole Response
    Thomas Edlind; Fiscal Year: 2002
    ..Finally, the signalling pathway responsible for azole-dependent upregulation of ERG expression will be elucidated with a focus on Rox1p and Haplp transcription factors. ..
  10. ANTIFUNGAL RESPONSE--MDR INDUCTION
    Thomas Edlind; Fiscal Year: 2002
    ..Candida homologues of these factors will be identified by PCR or expression in S. cerevisiae. Their role in MDR induction will be examined by gene disruption. ..
  11. Signaling Pathways in Yeast Azole Response
    Thomas Edlind; Fiscal Year: 2001
    ..Finally, the signalling pathway responsible for azole-dependent upregulation of ERG expression will be elucidated with a focus on Rox1p and Haplp transcription factors. ..
  12. ANTIFUNGAL RESPONSE--MDR INDUCTION
    Thomas Edlind; Fiscal Year: 2001
    ..Candida homologues of these factors will be identified by PCR or expression in S. cerevisiae. Their role in MDR induction will be examined by gene disruption. ..
  13. ANTIFUNGAL RESPONSE--MDR INDUCTION
    Thomas Edlind; Fiscal Year: 2000
    ..Candida homologues of these factors will be identified by PCR or expression in S. cerevisiae. Their role in MDR induction will be examined by gene disruption. ..
  14. Mutational Analysis of Fks1: Intrinsic Echinocandin Resistance
    Thomas D Edlind; Fiscal Year: 2010
    ..These studies will accelerate the development of second generation ECs that provide a broader spectrum of protection from fungal pathogens. ..