Timothy M Block

Summary

Affiliation: Drexel University
Country: USA

Publications

  1. ncbi request reprint The degree of readiness of selected biomarkers for the early detection of hepatocellular carcinoma: notes from a recent workshop
    Timothy M Block
    Drexel Institute for Biotechnology and Virology Research, Drexel University College of Medicine, Doylestown, PA 18902, USA
    Cancer Biomark 4:19-33. 2008
  2. pmc Identification of five interferon-induced cellular proteins that inhibit west nile virus and dengue virus infections
    Dong Jiang
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania 18902, USA
    J Virol 84:8332-41. 2010
  3. doi request reprint N-Alkyldeoxynojirimycin derivatives with novel terminal tertiary amide substitution for treatment of bovine viral diarrhea virus (BVDV), Dengue, and Tacaribe virus infections
    Yanming Du
    Institute for Hepatitis and Virus Research, 3805 Old Easton Road, Doylestown, PA 18902, USA
    Bioorg Med Chem Lett 23:2172-6. 2013
  4. pmc Identification of three interferon-inducible cellular enzymes that inhibit the replication of hepatitis C virus
    Dong Jiang
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, 3805 Old Easton Road, Doylestown, PA 18902, USA
    J Virol 82:1665-78. 2008
  5. pmc Sulfamoylbenzamide derivatives inhibit the assembly of hepatitis B virus nucleocapsids
    Matthew R Campagna
    Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA
    J Virol 87:6931-42. 2013
  6. pmc Regulation of hepatitis B virus replication by the phosphatidylinositol 3-kinase-akt signal transduction pathway
    Haitao Guo
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, 3805 Old Easton Road, Doylestown, PA 18902, USA
    J Virol 81:10072-80. 2007
  7. doi request reprint Design and synthesis of N-alkyldeoxynojirimycin derivatives with improved metabolic stability as inhibitors of BVDV and Tacaribe virus
    Yanming Du
    Institute for Hepatitis and Virus Research, 3805 Old Easton Road, Doylestown, PA 18902, USA
    Bioorg Med Chem Lett 23:4258-62. 2013
  8. ncbi request reprint Herpes simplex virus type 1 ICP4 deletion mutant virus d120 infection failed to induce apoptosis in nerve growth factor-differentiated PC12 cells
    Benjamas Aiamkitsumrit
    Drexel Institute for Biotechnology and Virology Research and Department of Microbiology and Immunology, College of Medicine, Drexel University, Doylestown, Pennsylvania, USA
    J Neurovirol 13:305-14. 2007
  9. pmc Identification of disubstituted sulfonamide compounds as specific inhibitors of hepatitis B virus covalently closed circular DNA formation
    Dawei Cai
    Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA
    Antimicrob Agents Chemother 56:4277-88. 2012
  10. pmc Design, synthesis, and biological evaluation of N-alkylated deoxynojirimycin (DNJ) derivatives for the treatment of dengue virus infection
    Wenquan Yu
    Institute for Hepatitis and Virus Research, Hepatitis B Foundation, Doylestown, PA 18902, USA
    J Med Chem 55:6061-75. 2012

Detail Information

Publications85

  1. ncbi request reprint The degree of readiness of selected biomarkers for the early detection of hepatocellular carcinoma: notes from a recent workshop
    Timothy M Block
    Drexel Institute for Biotechnology and Virology Research, Drexel University College of Medicine, Doylestown, PA 18902, USA
    Cancer Biomark 4:19-33. 2008
    ..It is emphasized that only a selected set of biomarkers was considered; thus, this review is not comprehensive and not intended to review all candidate HCC biomarkers...
  2. pmc Identification of five interferon-induced cellular proteins that inhibit west nile virus and dengue virus infections
    Dong Jiang
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania 18902, USA
    J Virol 84:8332-41. 2010
    ..Our results thus imply that the antiviral activity of IFN-alpha is collectively mediated by a panel of ISGs that disrupt multiple steps of the DENV and WNV life cycles...
  3. doi request reprint N-Alkyldeoxynojirimycin derivatives with novel terminal tertiary amide substitution for treatment of bovine viral diarrhea virus (BVDV), Dengue, and Tacaribe virus infections
    Yanming Du
    Institute for Hepatitis and Virus Research, 3805 Old Easton Road, Doylestown, PA 18902, USA
    Bioorg Med Chem Lett 23:2172-6. 2013
    ..Further optimization resulted in a structurally more stable lead compound 24 with a submicromolar EC50 against BVDV, Dengue, and Tacaribe; and low cytotoxicity...
  4. pmc Identification of three interferon-inducible cellular enzymes that inhibit the replication of hepatitis C virus
    Dong Jiang
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, 3805 Old Easton Road, Doylestown, PA 18902, USA
    J Virol 82:1665-78. 2008
    ..Our findings suggest that viperin represents a novel antiviral pathway that works together with other antiviral proteins, such as ISG20 and PKR, to mediate the IFN response against HCV infection...
  5. pmc Sulfamoylbenzamide derivatives inhibit the assembly of hepatitis B virus nucleocapsids
    Matthew R Campagna
    Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA
    J Virol 87:6931-42. 2013
    ..In summary, SBAs represent a novel chemical entity with superior activity and a unique antiviral mechanism and are thus warranted for further development as novel antiviral therapeutics for the treatment of chronic hepatitis B...
  6. pmc Regulation of hepatitis B virus replication by the phosphatidylinositol 3-kinase-akt signal transduction pathway
    Haitao Guo
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, 3805 Old Easton Road, Doylestown, PA 18902, USA
    J Virol 81:10072-80. 2007
    ....
  7. doi request reprint Design and synthesis of N-alkyldeoxynojirimycin derivatives with improved metabolic stability as inhibitors of BVDV and Tacaribe virus
    Yanming Du
    Institute for Hepatitis and Virus Research, 3805 Old Easton Road, Doylestown, PA 18902, USA
    Bioorg Med Chem Lett 23:4258-62. 2013
    ....
  8. ncbi request reprint Herpes simplex virus type 1 ICP4 deletion mutant virus d120 infection failed to induce apoptosis in nerve growth factor-differentiated PC12 cells
    Benjamas Aiamkitsumrit
    Drexel Institute for Biotechnology and Virology Research and Department of Microbiology and Immunology, College of Medicine, Drexel University, Doylestown, Pennsylvania, USA
    J Neurovirol 13:305-14. 2007
    ..The possible mechanisms involved in the failure of d120 to induce apoptosis in neuronal-like NGF-differentiated PC12 cells are discussed...
  9. pmc Identification of disubstituted sulfonamide compounds as specific inhibitors of hepatitis B virus covalently closed circular DNA formation
    Dawei Cai
    Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA
    Antimicrob Agents Chemother 56:4277-88. 2012
    ....
  10. pmc Design, synthesis, and biological evaluation of N-alkylated deoxynojirimycin (DNJ) derivatives for the treatment of dengue virus infection
    Wenquan Yu
    Institute for Hepatitis and Virus Research, Hepatitis B Foundation, Doylestown, PA 18902, USA
    J Med Chem 55:6061-75. 2012
    ..In male Sprague-Dawley rats, compound 3l was well tolerated at a dose up to 200 mg/kg and displayed desirable PK profiles, with significantly improved bioavailability (F = 92 ± 4%)...
  11. pmc Direct evidence that HSV DNA damaged by ultraviolet (UV) irradiation can be repaired in a cell type-dependent manner
    Scott Millhouse
    Drexel Institute for Biotechnology and Virology Research and Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PA 18902, USA
    J Neurovirol 18:231-43. 2012
    ..The possible significance of varying cell type mediated repair of viral DNA to viral pathogenesis is discussed...
  12. pmc Interferons accelerate decay of replication-competent nucleocapsids of hepatitis B virus
    Chunxiao Xu
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, 3805 Old Easton Road, Doylestown, PA 18902, USA
    J Virol 84:9332-40. 2010
    ..Our findings thus reveal a novel antiviral mechanism of IFNs and provide a basis for a better understanding of HBV pathobiology...
  13. ncbi request reprint N-linked glycosylation of the liver cancer biomarker GP73
    Pamela A Norton
    Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania 48902, USA
    J Cell Biochem 104:136-49. 2008
    ....
  14. pmc Glucosidase inhibition enhances presentation of de-N-glycosylated hepatitis B virus epitopes by major histocompatibility complex class I in vitro and in woodchucks
    Pamela A Norton
    Drexel Institute for Biotechnology and Virology Research and Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PA, USA
    Hepatology 52:1242-50. 2010
    ....
  15. pmc Alkylated porphyrins have broad antiviral activity against hepadnaviruses, flaviviruses, filoviruses, and arenaviruses
    Haitao Guo
    Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PA 18902, USA
    Antimicrob Agents Chemother 55:478-86. 2011
    ..The possible application of this family of compounds as antiviral agents, as microbicides and systemic virus neutralizing agents, is discussed...
  16. pmc Characterization of the intracellular deproteinized relaxed circular DNA of hepatitis B virus: an intermediate of covalently closed circular DNA formation
    Haitao Guo
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PA 18902, USA
    J Virol 81:12472-84. 2007
    ....
  17. pmc Increased levels of galactose-deficient anti-Gal immunoglobulin G in the sera of hepatitis C virus-infected individuals with fibrosis and cirrhosis
    Anand S Mehta
    Drexel University College of Medicine and Department of Microbiology and Immunology and Drexel Institute for Biotechnology and Virology, Doylestown, PA 18901, USA
    J Virol 82:1259-70. 2008
    ..The reason for the alteration in the glycosylation of anti-Gal IgG is currently unclear but may be related to the natural history of the disease and may be useful in the noninvasive detection of fibrosis and cirrhosis...
  18. pmc Activation of pattern recognition receptor-mediated innate immunity inhibits the replication of hepatitis B virus in human hepatocyte-derived cells
    Haitao Guo
    Department of Microbiology and Immunology, Drexel Institute for Biotechnology and Virology Research, Drexel University College of Medicine, Doylestown, Pennsylvania 18901, USA
    J Virol 83:847-58. 2009
    ....
  19. pdf Fucosylated glycoproteins as markers of liver disease
    Anand Mehta
    Department of Microbiology and Immunology, Drexel University College of Medicine, Drexel Institute for Biotechnology and Virology, 3805 Old Easton Road, Doylestown, PA 18902, USA
    Dis Markers 25:259-65. 2008
    ..The mechanisms by which changes in glycosylation are observed and their use as biomarkers of disease will be discussed...
  20. pmc Liver-specific microRNA miR-122 enhances the replication of hepatitis C virus in nonhepatic cells
    Jinhong Chang
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, 3805 Old Easton Road, Doylestown, PA 18902, USA
    J Virol 82:8215-23. 2008
    ..Therefore, we conclude that although miR-122 is not absolutely required, it greatly enhances HCV replication in nonhepatic cells...
  21. pmc RO 90-7501 enhances TLR3 and RLR agonist induced antiviral response
    Fang Guo
    Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, United States of America
    PLoS ONE 7:e42583. 2012
    ..Our results thus imply that pharmacological modulation of PRR signal transduction pathways in favor of the induction of a beneficial antiviral response can be a novel therapeutic strategy...
  22. pmc Quantification of complex precore mutations of hepatitis B virus by SimpleProbe real time PCR and dual melting analysis
    Hui Nie
    Department of Mcrobiology and Immunology, Drexel University College of Medicine, Doylestown, PA 18902, United States
    J Clin Virol 51:234-40. 2011
    ..However, a reliable method to quantify either mutant is not available, partly because the viral genome has polymorphisms in general and the precore mutations are complex...
  23. pmc Inhibition of cellular alpha-glucosidases results in increased presentation of hepatitis B virus glycoprotein-derived peptides by MHC class I
    Ender Simsek
    Department of Microbiology and Immunology, Drexel Institute for Biotechnology and Virology Research, Drexel University College of Medicine, Doylestown, PA 18902, USA
    Virology 384:12-5. 2009
    ....
  24. pmc Novel imino sugar derivatives demonstrate potent antiviral activity against flaviviruses
    Jinhong Chang
    Department of Microbiology and Immunology, Drexel Institute for Biotechnology and Virology Research, Drexel University College of Medicine, Doylestown, Pennsylvania 18902, USA
    Antimicrob Agents Chemother 53:1501-8. 2009
    ..Therefore, these compounds represent the best in their class and may offer realistic candidates for the development of antiviral therapeutics against human DENV infections...
  25. doi request reprint Novel fucosylated biomarkers for the early detection of hepatocellular carcinoma
    Mengjun Wang
    Department of Microbiology and Immunology, Drexel Institute for Biotechnology and Virology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA
    Cancer Epidemiol Biomarkers Prev 18:1914-21. 2009
    ..94. In conclusion, the altered glycosylation of serum glycoproteins can act as potential biomarkers of primary HCC when used independently or in combination with other markers of HCC...
  26. pmc Use of targeted glycoproteomics to identify serum glycoproteins that correlate with liver cancer in woodchucks and humans
    Timothy M Block
    Drexel Institute for Biotechnology and Virology Research, Drexel University, Doylestown, PA 18901, USA
    Proc Natl Acad Sci U S A 102:779-84. 2005
    ..The potential of this technology for biomarker discovery and the implications of increased levels of GP73 in liver cancer are discussed...
  27. ncbi request reprint Proteomic analysis of serum associated fucosylated glycoproteins in the development of primary hepatocellular carcinoma
    Mary Ann Comunale
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania 18901, USA
    J Proteome Res 5:308-15. 2006
    ..In total, 19 proteins were found to be hyperfucosylated in cancer. The potential of these proteins as biomarkers of cancer is discussed...
  28. pmc Detection of mutated K-ras DNA in urine, plasma, and serum of patients with colorectal carcinoma or adenomatous polyps
    Ying Hsiu Su
    Department of Microbiology and Immunology, College of Medicine, Drexel University, Doylestown, Pennsylvania, USA
    Ann N Y Acad Sci 1137:197-206. 2008
    ..0005), suggesting that inhibitory factors in serum/plasma may be more limiting than in urine. The use and practicality of urine as a source of circulating DNA for cancer detection are discussed...
  29. ncbi request reprint The stability of herpes simplex virus type I genomes in infected Vero cells undergoing viral induced apoptosis
    Ying Hsiu Su
    Department of Microbiology and Immunology, College of Medicine, Drexel University, Doylestown, Pennsylvania 18901 2697, USA
    J Neurovirol 12:375-86. 2006
    ..The possibility that the genome structure and replication compartment formation provide protection to the HSV-1 genome from degradation is discussed...
  30. pmc Chronic hepatitis B: what should be the goal for new therapies?
    Timothy M Block
    Department of Microbiology and Immunology, Drexel University College of Medicine, 3805 Old Easton Road, Doylestown, PA 18902, USA
    Antiviral Res 98:27-34. 2013
    ..We suggest that such a virological cure should be the goal of future therapeutics in all patients with chronic hepatitis B. The extent to which a virological cure predicts a functional cure will only be determined by long-term follow-up...
  31. pmc A ubiquitin independent degradation pathway utilized by a hepatitis B virus envelope protein to limit antigen presentation
    Yuanjie Liu
    Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, United States of America
    PLoS ONE 6:e24477. 2011
    ....
  32. pmc Indoleamine 2,3-dioxygenase mediates the antiviral effect of gamma interferon against hepatitis B virus in human hepatocyte-derived cells
    Richeng Mao
    Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, 3805 Old Easton Road, Doylestown, PA 18902, USA
    J Virol 85:1048-57. 2011
    ....
  33. pmc Small molecule inhibitors of ER α-glucosidases are active against multiple hemorrhagic fever viruses
    Jinhong Chang
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PA 18902, United States
    Antiviral Res 98:432-40. 2013
    ..Our study thus proves the concept that imino sugars are promising drug candidates for the management of viral hemorrhagic fever caused by variety of viruses...
  34. ncbi request reprint Alpha interferon-induced antiviral response noncytolytically reduces replication defective adenovirus DNA in MDBK cells
    Ju Tao Guo
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PA 18902, USA
    Antiviral Res 76:232-40. 2007
    ..Our data suggest that IFN-alpha-induced antiviral program is able to discriminate host cellular DNA from episomal viral DNA and might represent a novel pathway of interferon mediate innate defense against DNA virus infections...
  35. pmc Molecular virology of hepatitis B virus for clinicians
    Timothy M Block
    Department of Microbiology and Immunology, Drexel University College of Medicine, 3805 Old Easton Road, Doylestown, PA 18902, USA
    Clin Liver Dis 11:685-706, vii. 2007
    ..The way in which these steps may influence the natural history of viral pathogenesis, as well as the effectiveness of interventions, receives special consideration...
  36. pmc Inhibition of hepatitis B virus replication by the host zinc finger antiviral protein
    Richeng Mao
    Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, United States of America
    PLoS Pathog 9:e1003494. 2013
    ..Our findings thus shed light on virus-host interaction, viral pathogenesis, and antiviral approaches. ..
  37. pmc Interleukin-6 and oncostatin M are elevated in liver disease in conjunction with candidate hepatocellular carcinoma biomarker GP73
    Hongyan Liang
    Department of Microbiology and Immunology, Drexel Institute for Biotechnology and Virology Research, Drexel University College of Medicine, Doylestown, PA 18902, USA
    Cancer Biomark 11:161-71. 2012
    ..To our knowledge, this is the first report of OSM elevation being associated with liver disease...
  38. pmc Competitive inhibitor of cellular α-glucosidases protects mice from lethal dengue virus infection
    Jinhong Chang
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PA 18902, United States
    Antiviral Res 92:369-71. 2011
    ..Our results thus provide a strong support for the development of CM-10-18 or its derivatives as antiviral agents to treat servere dengue virus infections...
  39. pmc Production and function of the cytoplasmic deproteinized relaxed circular DNA of hepadnaviruses
    Haitao Guo
    Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PA 18902, USA
    J Virol 84:387-96. 2010
    ....
  40. pmc Hepatitis B and hepatitis C virus replication upregulates serine protease inhibitor Kazal, resulting in cellular resistance to serine protease-dependent apoptosis
    Jason Lamontagne
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology, Drexel University, 3805 Old Easton Road, Doylestown, PA 18902, USA
    J Virol 84:907-17. 2010
    ..These findings suggest a possibility by which HBV and HCV, two very different viruses, can share a common mechanism in provoking liver disease and cancer...
  41. pmc Antiviral effect of interferon lambda against West Nile virus
    Dongling Ma
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PA 18902, USA
    Antiviral Res 83:53-60. 2009
    ....
  42. pmc Evidence that herpes simplex virus DNA derived from quiescently infected cells in vitro, and latently infected cells in vivo, is physically damaged
    Scott Millhouse
    Drexel Institute for Biotechnology and Virology Research and Department of Microbiology and Immunology, College of Medicine, Drexel University, Doylestown, Pennsylvania 18901 2697, USA
    J Neurovirol 16:384-98. 2010
    ..Taken together, these results represent the first evidence that herpes simplex virus DNA is physically damaged as a function of long-term infection. Implications for viral reactivation and pathogenesis are discussed...
  43. ncbi request reprint Comparative proteomic analysis of de-N-glycosylated serum from hepatitis B carriers reveals polypeptides that correlate with disease status
    Mary Ann Comunale
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, The Jefferson Center, Doylestown, PA 18901, USA
    Proteomics 4:826-38. 2004
    ....
  44. pmc Removal of high-molecular-weight DNA by carboxylated magnetic beads enhances the detection of mutated K-ras DNA in urine
    Ying Hsiu Su
    Department of Microbiology and Immunology, College of Medicine, Drexel University, Doylestown, Pennsylvania, USA
    Ann N Y Acad Sci 1137:82-91. 2008
    ..The concurrence of K-ras mutations detected in disease tissue and the corresponding urine specimen is significantly higher (P= 0.0015) when the samples were enriched in low-MW DNA...
  45. pmc Characterization of the host factors required for hepadnavirus covalently closed circular (ccc) DNA formation
    Haitao Guo
    Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, United States of America
    PLoS ONE 7:e43270. 2012
    ....
  46. pmc Quantitative dynamics of hepatitis B basal core promoter and precore mutants before and after HBeAg seroconversion
    Hui Nie
    Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PA, USA
    J Hepatol 56:795-802. 2012
    ..However, the exact role of these mutants in HBeAg seroconversion remains unclear, partly because the evolution of these mutant viruses before and after seroconversion has not been well studied...
  47. pmc Detection of hypermethylated vimentin in urine of patients with colorectal cancer
    Benjamin P Song
    Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 18901, USA
    J Mol Diagn 14:112-9. 2012
    ..The detection of mVIM in urine was significantly associated with CRC compared with controls (P < 0.0001, by Fisher's exact test). A potential urine test for CRC screening using epigenetic markers is discussed...
  48. pmc Rapid and sensitive detection of hepatitis B virus 1762T/1764A double mutation from hepatocellular carcinomas using LNA-mediated PCR clamping and hybridization probes
    Xiangdong David Ren
    Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PA 18901, USA
    J Virol Methods 158:24-9. 2009
    ..Thus PCR amplification coupled with the melting curve analyses provides a quick, simple, and highly sensitive tool for the detection of this HBV double mutation...
  49. pmc Ultrasensitive quantification of hepatitis B virus A1762T/G1764A mutant by a SimpleProbe PCR using a wild-type-selective PCR blocker and a primer-blocker-probe partial-overlap approach
    Hui Nie
    Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA
    J Clin Microbiol 49:2440-8. 2011
    ..The combination of siPCR and the SimpleProbe real-time PCR enabled mutant quantification in the presence of a 100,000-fold excess of the WT DNA...
  50. ncbi request reprint Detection of a K-ras mutation in urine of patients with colorectal cancer
    Ying Hsiu Su
    Department of Microbiology and Immunology, College of Medicine, Drexel University, 700 E Butler Ave, Doylestown, PA 18901, USA
    Cancer Biomark 1:177-82. 2005
    ..However, only a 56% concurrence was observed when the matched plasma specimens were tested from these 20 patients. These results suggest that urine might be a better resource for detecting K-ras mutation in circulating DNA...
  51. doi request reprint The innate immune response to hepatitis B virus infection: implications for pathogenesis and therapy
    Jinhong Chang
    Department of Microbiology and Immunology, Drexel University College of Medicine, 3805 Old Easton Road, Doylestown, PA 18902, USA
    Antiviral Res 96:405-13. 2012
    ....
  52. pmc Interferon-induced cell membrane proteins, IFITM3 and tetherin, inhibit vesicular stomatitis virus infection via distinct mechanisms
    Jessica M Weidner
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, 3805 Old Easton Road, Doylestown, PA 18902, USA
    J Virol 84:12646-57. 2010
    ..Collectively, our work sheds light on the mechanisms by which tetherin and IFITM3 restrict infection with rhabdoviruses and possibly other pathogenic viruses...
  53. pmc A locked nucleic acid clamp-mediated PCR assay for detection of a p53 codon 249 hotspot mutation in urine
    Selena Y Lin
    Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA
    J Mol Diagn 13:474-84. 2011
    ..The possibility of using this novel TP53 249T assay to develop a urine or blood test for HCC screening is discussed...
  54. pmc Alpha-interferon suppresses hepadnavirus transcription by altering epigenetic modification of cccDNA minichromosomes
    Fei Liu
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, United States of America
    PLoS Pathog 9:e1003613. 2013
    ..Moreover, IFN-α treatment also induced a delayed response that appeared to accelerate the decay of cccDNA. Our studies have thus shed light on the molecular mechanism by which IFN-α noncytolytically controls hepadnavirus infection. ..
  55. ncbi request reprint Hepatitis B virus e antigen production is dependent upon covalently closed circular (ccc) DNA in HepAD38 cell cultures and may serve as a cccDNA surrogate in antiviral screening assays
    Tianlun Zhou
    Institute for Hepatitis Virus Research, Hepatitis B Foundation, 700 East Butler Avenue, Doylestown, PA 18901, USA
    Antiviral Res 72:116-24. 2006
    ..Therefore, the secretion of HBeAg by HepAD38 cells could potentially serve as a convenient reporter for the high throughput screening of novel antiviral drugs targeting HBV cccDNA...
  56. doi request reprint A southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures
    Dawei Cai
    Institute for Biotechnology and Virology Research, Drexel University College of Medicine, Doylestown, PA, USA
    Methods Mol Biol 1030:151-61. 2013
    ..The method is straightforward and reliable for cccDNA assay with cell culture samples, and it is useful for both HBV molecular biology and antiviral research. ..
  57. pmc alpha-Galactosylceramide and novel synthetic glycolipids directly induce the innate host defense pathway and have direct activity against hepatitis B and C viruses
    Anand S Mehta
    Department of Biochemistry, The Jefferson Center, Jefferson Medical College, 700 E Butler Ave, Doylestown, PA 18901, USA
    Antimicrob Agents Chemother 48:2085-90. 2004
    ..To exploit this activity, we have developed a new class of smaller, orally available glycolipids that also induce the innate host defense pathway and have direct activity against HBV and hepatitis C virus...
  58. ncbi request reprint A strategy for the comparative analysis of serum proteomes for the discovery of biomarkers for hepatocellular carcinoma
    Laura F Steel
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Jefferson Center for Biomedical Research, Doylestown, PA 18901, USA
    Proteomics 3:601-9. 2003
    ..These preliminary studies suggest that a proteomic methodology can be used for the identification of serum biomarkers for HCC and other liver disease...
  59. ncbi request reprint Assays for glucosidase inhibitors with potential antiviral activities: secreted alkaline phosphatase as a surrogate marker
    Pamela A Norton
    Jefferson Center for Biomedical Research, Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Doylestown, PA 18901, USA
    J Virol Methods 124:167-72. 2005
    ..Thus, measuring SEAP secretion may be another method for evaluating glucosidase inhibition. In addition, this finding has important implications for the use of a SEAP reporter in screens of potential antiviral agents...
  60. ncbi request reprint Does rapid oligomerization of hepatitis B envelope proteins play a role in resistance to proteasome degradation and enhance chronicity?
    Timothy M Block
    Department of Microbiology and Immunology, Drexel Institute for Biotechnology and Virology Research, Drexel University College of Medicine, Doylestown, Pennsylvania 18901, USA
    DNA Cell Biol 25:165-70. 2006
    ..The concept offers an explanation for the nearly unidirectional and rapid kinetics whereby HBV proteins form multimers and generate a surplus of viral structures that have not been thought to serve any useful structural purpose...
  61. pmc Combination of α-glucosidase inhibitor and ribavirin for the treatment of dengue virus infection in vitro and in vivo
    Jinhong Chang
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, 3805 Old Easton Road, Doylestown, PA 18902, United States
    Antiviral Res 89:26-34. 2011
    ..Our findings thus suggest that combination therapy of two broad-spectrum antiviral agents may provide a practically useful approach for the treatment of DENV infection...
  62. ncbi request reprint Inhibition of host ER glucosidase activity prevents Golgi processing of virion-associated bovine viral diarrhea virus E2 glycoproteins and reduces infectivity of secreted virions
    Robert Jordan
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Doylestown, Pennsylvania, 18901, USA
    Virology 295:10-9. 2002
    ....
  63. doi request reprint Tumor-associated protein SPIK/TATI suppresses serine protease dependent cell apoptosis
    Xuanyong Lu
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, College of Medicine, Drexel University, 3805 Old Easton Road, Doylestown, PA 18902, USA
    Apoptosis 13:483-94. 2008
    ..Previous work has associated SPIK and cancer development, indicating that this finding will help to open the doorway for further study on the mechanism of SPDCA and the role it may play in cancer development...
  64. pmc Antiviral activity of geneticin against dengue virus
    Xianchao G Zhang
    Institute of Hepatitis and Viral Research, Drexel University College of Medicine, Doylestown, PA 18902, USA
    Antiviral Res 83:21-7. 2009
    ..Neomycin and paromomycin demonstrated weak antiviral activity at high concentrations. Finally, aminoglycoside-3'-phosphotransferase activity of neomycin-resistant gene abolished antiviral activity of geneticin...
  65. pmc Construction of a herpes simplex virus type 1 mutant with only a three-nucleotide change in the branchpoint region of the latency-associated transcript (LAT) and the stability of its two-kilobase LAT intron
    Alan K Ng
    Department of Biochemistry and Molecular Pharmacology, Jefferson Center for Biomedical Research, Thomas Jefferson University, 700 E Butler Avenue, Doylestown, PA 18901 2697, USA
    J Virol 78:12097-106. 2004
    ..However, the ability of mutant Sy2 to reactivate from trigeminal ganglia (TG) derived from latently infected mice was indistinguishable from that of wild-type virus, as assayed in the mouse TG explant reactivation system...
  66. pmc Evidence that the immediate-early gene product ICP4 is necessary for the genome of the herpes simplex virus type 1 ICP4 deletion mutant strain d120 to circularize in infected cells
    Ying Hsiu Su
    Department of Microbiology and Immunology, Drexel Institute for Biotechnology and Virology Research, College of Medicine, Drexel University, 3805 Old Easton Road, Doylestown, PA 18901 2697, USA
    J Virol 80:11589-97. 2006
    ..Collectively, these data suggest that ICP4 protein has an important role in mediating the endless formation of the HSV-1 genome upon infection and that this function can be provided in trans...
  67. ncbi request reprint Discovery of small molecule inhibitors of West Nile virus using a high-throughput sub-genomic replicon screen
    Baohua Gu
    Drexel Institute for Biotechnology and Virology Research, Drexel University, College of Medicine, Doylestown, PA 18901, USA
    Antiviral Res 70:39-50. 2006
    ..These compounds should be valuable for developing anti-WNV therapeutic drugs as well as research tools to study the mechanism of WNV replication...
  68. ncbi request reprint Stability and circularization of herpes simplex virus type 1 genomes in quiescently infected PC12 cultures
    Ying Hsiu Su
    Jefferson Center for Biomedical Research of Thomas Jefferson University, 700 E Butler Ave, Doylestown, PA 18901 2697, USA
    J Gen Virol 83:2943-50. 2002
    ..The relevance to the virus life-cycle in neurones in vivo is discussed...
  69. ncbi request reprint Molecular viral oncology of hepatocellular carcinoma
    Timothy M Block
    Department of Molecular Pharmacology and Biochemistry, Jefferson Center for Biomedical Research of Thomas Jefferson University, 700 East Butler Ave, Doylestown, PA 18901, USA
    Oncogene 22:5093-107. 2003
    ..Viral functions may also play a more direct role in mediating oncogenesis. This review considers the molecular and cellular mechanisms involved in primary hepatocellular carcinoma, using a viral perspective...
  70. ncbi request reprint Transrenal DNA as a diagnostic tool: important technical notes
    Ying Hsiu Su
    Department of Biochemistry and Molecular Pharmacology, Jefferson Center for Biomedical Research, Thomas Jefferson University, Doylestown, Pennsylvania 18901, USA
    Ann N Y Acad Sci 1022:81-9. 2004
    ....
  71. pmc Human urine contains small, 150 to 250 nucleotide-sized, soluble DNA derived from the circulation and may be useful in the detection of colorectal cancer
    Ying Hsiu Su
    Department of Biochemistry and Molecular Pharmacology, Jefferson Center for Biomedical Research, Thomas Jefferson University, Doylestown, Pennsylvania, USA
    J Mol Diagn 6:101-7. 2004
    ..The possibility that the source of the trans renal DNA is apoptotic cells, and the potential use of this finding for cancer detection and monitoring is discussed...
  72. ncbi request reprint Preferential isolation of fragmented DNA enhances the detection of circulating mutated k-ras DNA
    Mengjun Wang
    Department of Biochemistry and Molecular Pharmacology Jefferson Center for Biomedical Research, Thomas Jefferson University, 700 E Butler Ave, Doylestown, PA 18901, USA
    Clin Chem 50:211-3. 2004
  73. pmc Structure-activity relationship of a new class of anti-hepatitis B virus agents
    Anand Mehta
    Department of Biochemistry and Molecular Pharmacology, The Jefferson Center, Jefferson Medical College, Doylestown, Pennsylvania 18901 2697, USA
    Antimicrob Agents Chemother 46:4004-8. 2002
    ..This work suggests that the antiviral activity of the alkovirs requires an alkyl chain length of at least eight carbons but that the galactose-based head group can be modified with little or no loss in activity...
  74. pmc The alkylated imino sugar, n-(n-Nonyl)-deoxygalactonojirimycin, reduces the amount of hepatitis B virus nucleocapsid in tissue culture
    Xuanyong Lu
    Biochemistry and Molecular Pharmacology Department, Jefferson Center for Bio Medical Research and Agricultural Medicine, Thomas Jefferson University, Doylestown, Pennsylvania 18901, USA
    J Virol 77:11933-40. 2003
    ..The similarities and differences between this alkylated imino sugar and these other mediators are discussed...
  75. ncbi request reprint Enhancement of infection of HepG2 cells in culture by predigestion of hepadnavirus with V8 protease
    Xuanyong Lu
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Doylestown, PA, USA
    Methods Mol Med 96:199-208. 2004
  76. ncbi request reprint Imino sugars that are less toxic but more potent as antivirals, in vitro, compared with N-n-nonyl DNJ
    Anand Mehta
    Department of Biochemistry and Molecular Pharmacology, The Jefferson Center of Thomas Jefferson University, Doylestown, PA, USA
    Antivir Chem Chemother 13:299-304. 2002
    ..The results of this antiviral survey and the implications for the mechanism of action and ultimate therapeutic potential of the DNJ-based imino sugars is provided and discussed...
  77. pmc Hepatitis B virus-mediated changes of apolipoprotein mRNA abundance in cultured hepatoma cells
    Pamela A Norton
    Jefferson Center for Biomedical Research and Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Doylestown, Pennsylvania, USA
    J Virol 77:5503-6. 2003
    ..Analysis of a third line containing an inducible viral genome implicated viral pregenomic RNA in apolipoprotein mRNA reduction. We conclude that HBV alters infected cells despite the absence of overt cytopathogenicity...
  78. pmc Replication of a cytopathic strain of bovine viral diarrhea virus activates PERK and induces endoplasmic reticulum stress-mediated apoptosis of MDBK cells
    Robert Jordan
    Department of Biochemistry and Molecular Pharmacology, The Jefferson Center for Biomedical Research, Thomas Jefferson University, Doylestown, Pennsylvania 18901, USA
    J Virol 76:9588-99. 2002
    ....
  79. ncbi request reprint Herpes simplex virus type 1 infection prevents detachment of nerve growth factor-differentiated PC12 cells in culture
    Michael J Moxley
    Jefferson Center for Biomedical Research of Thomas Jefferson University, Department of Biochemistry and Molecular Pharmacology, 700 E Butler Avenue, Doylestown, PA 18901 2697, USA
    J Gen Virol 83:1591-600. 2002
    ..These findings indicate that de novo viral gene synthesis mediates changes to the host NGF-differentiated PC12 cells, which results in prevention of detachment...
  80. ncbi request reprint The combination of interferon alpha-2b and n-butyl deoxynojirimycin has a greater than additive antiviral effect upon production of infectious bovine viral diarrhea virus (BVDV) in vitro: implications for hepatitis C virus (HCV) therapy
    Serguey Ouzounov
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Antiviral Res 55:425-35. 2002
    ..These data are consistent with an interpretation that glucosidase inhibitors and IFN have a synergistic antiviral effect in tissue culture. The relevance of these finding to treatment of HCV infection is discussed...
  81. ncbi request reprint alpha-Glucosidase inhibitors have a prolonged antiviral effect against hepatitis B virus through the sustained inhibition of the large and middle envelope glycoproteins
    Ender Simsek
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University College of Medicine, Philadelphia, PA, USA
    Antivir Chem Chemother 17:259-67. 2006
    ..The implications of the prolonged antiviral effect against HBV and the use of glucosidase inhibitors as antiviral agents are discussed...
  82. ncbi request reprint GP73, a resident Golgi glycoprotein, is a novel serum marker for hepatocellular carcinoma
    Jorge A Marrero
    Division of Gastroenterology, University of Michigan Medical Center, 3912 Taubman Center, Ann Arbor, MI 48109 0362, USA
    J Hepatol 43:1007-12. 2005
    ..Golgi protein-73 (GP73) is up-regulated in hepatocellular carcinoma (HCC). The aims of this study were to determine if GP73 is detected in the serum, and to establish the sensitivity and specificity of serum GP73 for diagnosing HCC...
  83. ncbi request reprint SELDI-TOF MS profiling of serum for detection of the progression of chronic hepatitis C to hepatocellular carcinoma
    E Ellen Schwegler
    Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, VA, USA
    Hepatology 41:634-42. 2005
    ..In conclusion, SELDI-TOF MS serum profiling is able to distinguish HCC from liver disease before cirrhosis as well as cirrhosis, especially in patients with HCV infection compared with other etiologies...
  84. ncbi request reprint Glyco- and peptidomimetics from three-component Joullié-Ugi coupling show selective antiviral activity
    Timothy M Chapman
    Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford UK, OX1 3TA
    J Am Chem Soc 127:506-7. 2005
    ..An observed selectivity for this HCV model over hepatitis B virus and remarkably low toxicity suggest a novel mode of action...
  85. pmc Human immunodeficiency virus-related microbial translocation and progression of hepatitis C
    Ashwin Balagopal
    Division of Infectious Diseases, Viral Hepatitis Center, Department of Medicine, The Johns Hopkins Medical Institutions, Baltimore, Maryland, USA
    Gastroenterology 135:226-33. 2008
    ..We hypothesized that HIV promotes liver disease by enhancing microbial translocation...