THOMAS JAMES SMITH

Summary

Affiliation: Donald Danforth Plant Science Center
Country: USA

Publications

  1. pmc Structural studies on antibody recognition and neutralization of viruses
    THOMAS JAMES SMITH
    Donald Danforth Plant Science Center, Saint Louis, MO 63132, USA
    Curr Opin Virol 1:150-6. 2011
  2. doi request reprint Untangling the glutamate dehydrogenase allosteric nightmare
    Thomas J Smith
    Donald Danforth Plant Science Center, Saint Louis, MO 63132, USA
    Trends Biochem Sci 33:557-64. 2008
  3. ncbi request reprint The structure of apo human glutamate dehydrogenase details subunit communication and allostery
    Thomas J Smith
    Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, MO 63132, USA
    J Mol Biol 318:765-77. 2002
  4. ncbi request reprint Evolution of glutamate dehydrogenase regulation of insulin homeostasis is an example of molecular exaptation
    Aron Allen
    The Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, Missouri 63132, USA
    Biochemistry 43:14431-43. 2004
  5. ncbi request reprint Green tea polyphenols modulate insulin secretion by inhibiting glutamate dehydrogenase
    Changhong Li
    Donald Danforth Plant Science Center, 975 Warson Road, St Louis, MO 63132, USA
    J Biol Chem 281:10214-21. 2006
  6. ncbi request reprint Structural studies on ADP activation of mammalian glutamate dehydrogenase and the evolution of regulation
    Soojay Banerjee
    Donald Danforth Plant Science Center, St Louis, Missouri 63132, USA
    Biochemistry 42:3446-56. 2003
  7. pmc Expression, purification and characterization of human glutamate dehydrogenase (GDH) allosteric regulatory mutations
    Jie Fang
    Division of Endocrinology, The Children s Hospital of Philadelphia, 34th Street and Civic Center Boulevard, Philadelphia, PA 19104, USA
    Biochem J 363:81-7. 2002
  8. pmc Mutagenesis of the "leucine gate" to explore the basis of catalytic versatility in soluble methane monooxygenase
    Elena Borodina
    Biomedical Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, United Kingdom
    Appl Environ Microbiol 73:6460-7. 2007
  9. pmc High throughput screening reveals several new classes of glutamate dehydrogenase inhibitors
    Ming Li
    Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, Missouri 63132, USA
    Biochemistry 46:15089-102. 2007
  10. ncbi request reprint Possible regulatory role for the histidine-rich loop in the zinc transport protein, ZnuA
    Baoxian Wei
    Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, Missouri 63132, USA
    Biochemistry 46:8734-43. 2007

Research Grants

  1. Towards a Vaccine for the Common Cold
    Thomas Smith; Fiscal Year: 2009
  2. Mechanisms of glutamate dehydrogenase allostery
    Thomas Smith; Fiscal Year: 2007
  3. STRUCTURAL STUDIES OF PROTEIN SUBUNITS
    Thomas Smith; Fiscal Year: 2004
  4. Towards a Vaccine for the Common Cold
    THOMAS JAMES SMITH; Fiscal Year: 2010

Collaborators

  • CHARLES ALFRED STANLEY
  • Wei Qin
  • Steven R Deitcher
  • Himadri B Pakrasi
  • Aron Allen
  • Nicole M Koropatkin
  • Soojay Banerjee
  • Jie Fang
  • Baoxian Wei
  • Maitrayee Bhattacharyya-Pakrasi
  • Amelia M Randich
  • Elena Borodina
  • Nicole Koropatkin
  • Ming Li
  • Umesh Katpally
  • Changhong Li
  • Jae Kwagh
  • Ekundayo K Adeosun
  • Robert G Spelbrink
  • Howard Dalton
  • John J Castronuovo
  • Valorie D Bowman
  • Anastasia J Callaghan
  • J Colin Murrell
  • Ron Reade
  • David W Koppenaal
  • Kelly Dryden
  • Tim Nichol
  • Marc G Dumont
  • Kishore Kakani
  • Heather W Collins
  • Franz M Matschinsky
  • Habiba Najafi
  • Nicolai M Doliba
  • Giles Velarde
  • Nejmi Dilmac
  • Anne Mette Hoberg
  • Robert Ford
  • Gregory H Hockerman
  • Dilip M Shah
  • Timothy Schmidt
  • Timothy S Baker
  • Elaine S Chase
  • Mortimer Poncz
  • Ray M Price
  • Keith L Perry
  • Xing Zhang
  • Paul R Chipman
  • Courtney M MacMullen
  • Betty Y L Hsu
  • Alexander W E Franz
  • Susan E Slade

Detail Information

Publications23

  1. pmc Structural studies on antibody recognition and neutralization of viruses
    THOMAS JAMES SMITH
    Donald Danforth Plant Science Center, Saint Louis, MO 63132, USA
    Curr Opin Virol 1:150-6. 2011
    ..Finally, the structure of cucumber mosaic virus bound to a loop involved in aphid transmission demonstrated the importance of the context of antigen presentation and what happens when an antibody binds near an icosahedral symmetry axis...
  2. doi request reprint Untangling the glutamate dehydrogenase allosteric nightmare
    Thomas J Smith
    Donald Danforth Plant Science Center, Saint Louis, MO 63132, USA
    Trends Biochem Sci 33:557-64. 2008
    ..Ciliates, the 'missing link' in GDH evolution, might have created the antenna to accommodate changing organelle functions and was refined in humans to, at least in part, link amino acid catabolism with insulin secretion...
  3. ncbi request reprint The structure of apo human glutamate dehydrogenase details subunit communication and allostery
    Thomas J Smith
    Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, MO 63132, USA
    J Mol Biol 318:765-77. 2002
    ..These results support the previous proposal that purines regulate GDH activity by altering the dynamics of the NAD binding domain. Finally, a possible structural mechanism for negative cooperativity is presented...
  4. ncbi request reprint Evolution of glutamate dehydrogenase regulation of insulin homeostasis is an example of molecular exaptation
    Aron Allen
    The Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, Missouri 63132, USA
    Biochemistry 43:14431-43. 2004
    ....
  5. ncbi request reprint Green tea polyphenols modulate insulin secretion by inhibiting glutamate dehydrogenase
    Changhong Li
    Donald Danforth Plant Science Center, 975 Warson Road, St Louis, MO 63132, USA
    J Biol Chem 281:10214-21. 2006
    ..These results demonstrate that EGCG, much like the activator of GDH (BCH), can facilitate dissecting the complex regulation of insulin secretion by pharmacologically modulating the effects of GDH...
  6. ncbi request reprint Structural studies on ADP activation of mammalian glutamate dehydrogenase and the evolution of regulation
    Soojay Banerjee
    Donald Danforth Plant Science Center, St Louis, Missouri 63132, USA
    Biochemistry 42:3446-56. 2003
    ..This suggests that there was some selective advantage of the antenna itself and that animals evolved new functions for GDH through the addition of allosteric regulation...
  7. pmc Expression, purification and characterization of human glutamate dehydrogenase (GDH) allosteric regulatory mutations
    Jie Fang
    Division of Endocrinology, The Children s Hospital of Philadelphia, 34th Street and Civic Center Boulevard, Philadelphia, PA 19104, USA
    Biochem J 363:81-7. 2002
    ....
  8. pmc Mutagenesis of the "leucine gate" to explore the basis of catalytic versatility in soluble methane monooxygenase
    Elena Borodina
    Biomedical Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, United Kingdom
    Appl Environ Microbiol 73:6460-7. 2007
    ..Thus, our results indicate that the "leucine gate" in sMMO is more important in controlling the precision of regioselectivity than the sizes of substrates that can enter the active site...
  9. pmc High throughput screening reveals several new classes of glutamate dehydrogenase inhibitors
    Ming Li
    Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, Missouri 63132, USA
    Biochemistry 46:15089-102. 2007
    ..Therefore, these new compounds not only are new leads in the treatment of hyperactive GDH but also are useful in dissecting the complex allosteric nature of the enzyme...
  10. ncbi request reprint Possible regulatory role for the histidine-rich loop in the zinc transport protein, ZnuA
    Baoxian Wei
    Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, Missouri 63132, USA
    Biochemistry 46:8734-43. 2007
    ..From these results, we suggest that this region might be a sensor for high periplasmic levels of zinc...
  11. ncbi request reprint The structure of a cyanobacterial bicarbonate transport protein, CmpA
    Nicole M Koropatkin
    Donald Danforth Plant Science Center, St Louis, Missouri 63132, USA
    J Biol Chem 282:2606-14. 2007
    ..The binding of bicarbonate and metal appears to be highly cooperative and suggests that CmpA may co-transport bicarbonate and calcium or that calcium acts a cofactor in bicarbonate transport...
  12. ncbi request reprint Structures of T=1 and T=3 particles of cucumber necrosis virus: evidence of internal scaffolding
    Umesh Katpally
    Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, MO 63132, USA
    J Mol Biol 365:502-12. 2007
    ..Therefore, this region apparently has remarkably diverse functions that may be distributed unevenly among the quasi-equivalent A, B, and C subunits...
  13. pmc Atomic structure of a nitrate-binding protein crucial for photosynthetic productivity
    Nicole M Koropatkin
    Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, MO 63132, USA
    Proc Natl Acad Sci U S A 103:9820-5. 2006
    ..Interestingly, the residues lining the ligand binding pockets suggest that they both bind nitrate. This implies that the nitrogen and carbon uptake pathways are synchronized by intracellular nitrate and nitrite...
  14. ncbi request reprint The structure of the iron-binding protein, FutA1, from Synechocystis 6803
    Nicole Koropatkin
    Donald Danforth Plant Science Center, 975 North Warson Road, Saint Louis, MO 63132, USA
    J Biol Chem 282:27468-77. 2007
    ..Finally, neither FutA1 nor FutA2 binds iron as a siderophore complex or in the presence of anions, and both preferentially bind ferrous over ferric ions...
  15. pmc Differential antifungal and calcium channel-blocking activity among structurally related plant defensins
    Robert G Spelbrink
    The Donald Danforth Plant Science Center, St Louis, MO 63132, USA
    Plant Physiol 135:2055-67. 2004
    ....
  16. ncbi request reprint Dose-ranging trial with a recombinant urokinase (urokinase alfa) for occluded central venous catheters in oncology patients
    Steven R Deitcher
    Section of Hematology and Coagulation Medicine, The Cleveland Clinic Foundation, Cleveland, Ohio, USA
    J Vasc Interv Radiol 15:575-80. 2004
    ....
  17. ncbi request reprint Formaldehyde dehydrogenase preparations from Methylococcus capsulatus (Bath) comprise methanol dehydrogenase and methylene tetrahydromethanopterin dehydrogenase
    Ekundayo K Adeosun
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK
    Microbiology 150:707-13. 2004
    ..These results support previous findings that methylotrophs possess multiple pathways for formaldehyde dissimilation...
  18. ncbi request reprint Structural determinants of metal specificity in the zinc transport protein ZnuA from synechocystis 6803
    Soojay Banerjee
    Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, MO 63132, USA
    J Mol Biol 333:1061-9. 2003
    ..Therefore, while Mn2+ transporters can bind Zn2+ in vitro they may not be able to acquire it in vivo without this structure because of the low concentration of free Zn2+...
  19. ncbi request reprint Structural studies on antibody-virus complexes
    Thomas J Smith
    Donald Danforth Plant Science Center, St Louis, Missouri 63132, USA
    Adv Protein Chem 64:409-53. 2003
  20. pmc An antibody to the putative aphid recognition site on cucumber mosaic virus recognizes pentons but not hexons
    Valorie D Bowman
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Virol 76:12250-8. 2002
    ..Antibodies with such character have potential use as probes of viral assembly. Our results may provide an additional rationale for designing synthetic vaccines by using symmetrical viral particles...
  21. pmc Improved system for protein engineering of the hydroxylase component of soluble methane monooxygenase
    Thomas J Smith
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom
    Appl Environ Microbiol 68:5265-73. 2002
    ..With these results, we have demonstrated proof of the principle for protein engineering of this uniquely versatile enzyme...
  22. ncbi request reprint Residues near the N-terminus of protein B control autocatalytic proteolysis and the activity of soluble methane mono-oxygenase
    Anastasia J Callaghan
    Department of Biological Sciences, University of Warwick, Coventry, UK
    Eur J Biochem 269:1835-43. 2002
    ....
  23. ncbi request reprint Validation of an in vitro model of human saphenous vein hyperplasia
    John J Castronuovo
    Department of Surgery, Reeves Laboratory for Surgical Research, Morristown Memorial Hospital, 100 Madison Ave, Morristown, NJ 07962 1956, USA
    J Vasc Surg 35:152-7. 2002
    ....

Research Grants10

  1. Towards a Vaccine for the Common Cold
    Thomas Smith; Fiscal Year: 2009
    ..If successful, the methods developed here will also yield a rapid way to identify potential, conserved, vaccine targets for other antigenically diverse viruses as well. ..
  2. Mechanisms of glutamate dehydrogenase allostery
    Thomas Smith; Fiscal Year: 2007
    ....
  3. STRUCTURAL STUDIES OF PROTEIN SUBUNITS
    Thomas Smith; Fiscal Year: 2004
    ..These studies have the potential of changing the way we view the early steps in the viral infection process and will allow us to develop tools that are applicable to other viral systems. ..
  4. Towards a Vaccine for the Common Cold
    THOMAS JAMES SMITH; Fiscal Year: 2010
    ..If successful, the methods developed here will also yield a rapid way to identify potential, conserved, vaccine targets for other antigenically diverse viruses as well. ..