Mangala Rao

Summary

Affiliation: Division of Retrovirology
Country: USA

Publications

  1. pmc HIV-1 envelope resistance to proteasomal cleavage: implications for vaccine induced immune responses
    Nicholas J Steers
    United States Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America
    PLoS ONE 7:e42579. 2012
  2. ncbi request reprint HIV-1 variable loop 2 and its importance in HIV-1 infection and vaccine development
    Mangala Rao
    Laboratory of Adjuvant and Antigen Research, USMHRP at the Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Rm 2A08, Sliver Spring, MD 20910, USA
    Curr HIV Res 11:427-38. 2013
  3. ncbi request reprint Functional microtubules are required for antigen processing by macrophages and dendritic cells
    Kristina K Peachman
    Department of Membrane Biochemistry, Division of Biochemistry, Walter Reed Army Institute of Research, 503 Robert Grant Road, Silver Spring, MD 20910 7500, USA
    Immunol Lett 95:13-24. 2004
  4. pmc Anthrax vaccine antigen-adjuvant formulations completely protect New Zealand white rabbits against challenge with Bacillus anthracis Ames strain spores
    Kristina K Peachman
    U S Military HIV Research Program, Henry M Jackson Foundation for the Advancement of Military Medicine, Rockville, Maryland, USA
    Clin Vaccine Immunol 19:11-6. 2012
  5. doi request reprint Liposome-encapsulated HIV-1 Gag p24 containing lipid A induces effector CD4+ T-cells, memory CD8+ T-cells, and pro-inflammatory cytokines
    Nicholas J Steers
    Division of Retrovirology, USMHRP, Walter Reed Army Institute of Research, Rockville, MD 20850, USA
    Vaccine 27:6939-49. 2009
  6. ncbi request reprint Human dendritic cells and macrophages exhibit different intracellular processing pathways for soluble and liposome-encapsulated antigens
    Kristina K Peachman
    Department of Vaccine Production and Delivery, Division of Retrovirology, Walter Reed Army Institute of Research, US Military HIV Research Program, 13 Taft Court Suite 200, Rockville, MD 20850, USA
    Immunobiology 210:321-33. 2005
  7. doi request reprint Modulation of immunoproteasome subunits by liposomal lipid A
    Nicholas J Steers
    Division of Retrovirology, US Military HIV Research Program, Walter Reed Army Institute of Research, 1600 Gude Drive, Rockville, MD 20850, USA
    Vaccine 26:2849-59. 2008
  8. ncbi request reprint Liposome-stabilized oil-in-water emulsions as adjuvants: increased emulsion stability promotes induction of cytotoxic T lymphocytes against an HIV envelope antigen
    Roberta L Richards
    Department of Membrane Biochemistry, Walter Reed Army Institute of Research, Silver Spring, MD 20910 7500, USA
    Immunol Cell Biol 82:531-8. 2004
  9. ncbi request reprint Antibodies induced by liposomal protein exhibit dual binding to protein and lipid epitopes
    Nicos Karasavvas
    Division of Retrovirology, Walter Reed Army Institute of Research, US Military HIV Research Program, Department of Vaccine Production and Delivery, 1600 East Gude Drive, Rockville, MD 20850, USA
    Biochem Biophys Res Commun 366:982-7. 2008
  10. pmc Human immunodeficiency virus type 1 Gag p24 alters the composition of immunoproteasomes and affects antigen presentation
    Nicholas J Steers
    Henry M Jackson Foundation, Division of Retrovirology, USMHRP, Walter Reed Army Institute of Research, Rockville, MD 20850, USA
    J Virol 83:7049-61. 2009

Collaborators

Detail Information

Publications29

  1. pmc HIV-1 envelope resistance to proteasomal cleavage: implications for vaccine induced immune responses
    Nicholas J Steers
    United States Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America
    PLoS ONE 7:e42579. 2012
    ..Preliminary immunological data from the RV144 phase III trial indicated that the immune responses were biased towards the Env antigen with a dominant CD4+ T-cell response...
  2. ncbi request reprint HIV-1 variable loop 2 and its importance in HIV-1 infection and vaccine development
    Mangala Rao
    Laboratory of Adjuvant and Antigen Research, USMHRP at the Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Rm 2A08, Sliver Spring, MD 20910, USA
    Curr HIV Res 11:427-38. 2013
    ..Immunogens that can induce these antibodies should therefore be taken into consideration when designing HIV-1 vaccine formulations. ..
  3. ncbi request reprint Functional microtubules are required for antigen processing by macrophages and dendritic cells
    Kristina K Peachman
    Department of Membrane Biochemistry, Division of Biochemistry, Walter Reed Army Institute of Research, 503 Robert Grant Road, Silver Spring, MD 20910 7500, USA
    Immunol Lett 95:13-24. 2004
    ..These data suggest that trafficking of soluble and liposome-encapsulated ovalbumin requires a functional microtubule-dependent translocation system...
  4. pmc Anthrax vaccine antigen-adjuvant formulations completely protect New Zealand white rabbits against challenge with Bacillus anthracis Ames strain spores
    Kristina K Peachman
    U S Military HIV Research Program, Henry M Jackson Foundation for the Advancement of Military Medicine, Rockville, Maryland, USA
    Clin Vaccine Immunol 19:11-6. 2012
    ..In summary, the needle-free skin delivery and liposomal formulation that were found to be effective in two different animal model systems appear to be promising candidates for next-generation anthrax vaccine development...
  5. doi request reprint Liposome-encapsulated HIV-1 Gag p24 containing lipid A induces effector CD4+ T-cells, memory CD8+ T-cells, and pro-inflammatory cytokines
    Nicholas J Steers
    Division of Retrovirology, USMHRP, Walter Reed Army Institute of Research, Rockville, MD 20850, USA
    Vaccine 27:6939-49. 2009
    ..The results demonstrate the importance of the adjuvant liposomal lipid A for the induction of HIV-1 Gag p24 -specific CD8+ T-cells, effector CD4+ T-cells, and cytokines with a Th-1 type profile after immunization with L(p24+LA)...
  6. ncbi request reprint Human dendritic cells and macrophages exhibit different intracellular processing pathways for soluble and liposome-encapsulated antigens
    Kristina K Peachman
    Department of Vaccine Production and Delivery, Division of Retrovirology, Walter Reed Army Institute of Research, US Military HIV Research Program, 13 Taft Court Suite 200, Rockville, MD 20850, USA
    Immunobiology 210:321-33. 2005
    ..These studies demonstrate that the intracellular fate of soluble and liposomal antigens can differ depending upon the antigen-presenting cell...
  7. doi request reprint Modulation of immunoproteasome subunits by liposomal lipid A
    Nicholas J Steers
    Division of Retrovirology, US Military HIV Research Program, Walter Reed Army Institute of Research, 1600 Gude Drive, Rockville, MD 20850, USA
    Vaccine 26:2849-59. 2008
    ..L(LA) therefore appears to act as an intracellular adjuvant by upregulating the antigen processing machinery, which could result in efficient antigen presentation...
  8. ncbi request reprint Liposome-stabilized oil-in-water emulsions as adjuvants: increased emulsion stability promotes induction of cytotoxic T lymphocytes against an HIV envelope antigen
    Roberta L Richards
    Department of Membrane Biochemistry, Walter Reed Army Institute of Research, Silver Spring, MD 20910 7500, USA
    Immunol Cell Biol 82:531-8. 2004
    ..We conclude that stable liposomal oil-in-water emulsions provide an effective means of obtaining both antibody and CTL responses against an HIV envelope antigen...
  9. ncbi request reprint Antibodies induced by liposomal protein exhibit dual binding to protein and lipid epitopes
    Nicos Karasavvas
    Division of Retrovirology, Walter Reed Army Institute of Research, US Military HIV Research Program, Department of Vaccine Production and Delivery, 1600 East Gude Drive, Rockville, MD 20850, USA
    Biochem Biophys Res Commun 366:982-7. 2008
    ..Surface plasmon resonance studies with the former antibody showed that the liposomal cholesterol and liposomal gp140 each contributed to the overall binding energy of the antibody to liposomes containing cholesterol and protein...
  10. pmc Human immunodeficiency virus type 1 Gag p24 alters the composition of immunoproteasomes and affects antigen presentation
    Nicholas J Steers
    Henry M Jackson Foundation, Division of Retrovirology, USMHRP, Walter Reed Army Institute of Research, Rockville, MD 20850, USA
    J Virol 83:7049-61. 2009
    ..These results suggest that early antigen processing kinetics could influence the immunogenicity of CD8(+) T-cell epitopes generated...
  11. doi request reprint The importance of antibody isotype in HIV-1 virus capture assay and in TZM-bl neutralization
    Kristina K Peachman
    Henry M Jackson Foundation for the Advancement of Military Medicine, Rockville, Maryland, USA
    Viral Immunol 23:627-32. 2010
    ..Studies on the relationship between virus binding and neutralization in a TZM-bl pseudovirus assay indicated that in most cases, mAbs that exhibited neutralization also bound the virus...
  12. doi request reprint Infection of human peripheral blood mononuclear cells by erythrocyte-bound HIV-1: effects of antibodies and complement
    Zoltan Beck
    Division of Retrovirology, US Military HIV Research Program, Walter Reed Army Institute of Research, Rockville, MD 20850, USA
    Virology 412:441-7. 2011
    ....
  13. ncbi request reprint Designing the epitope flanking regions for optimal generation of CTL epitopes
    Nicholas J Steers
    United States Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA Henry M Jackson Foundation for the Advancement of Military Medicine, Rockledge, MD 20852, USA
    Vaccine 32:3509-16. 2014
    ..These results highlight the importance of flanking regions in promoting efficient antigen processing and presentation. This concept can have important implications in vaccine design and development strategies. ..
  14. doi request reprint Lipid A and liposomes containing lipid A as antigens and adjuvants
    Carl R Alving
    Department of Adjuvant and Antigen Research, U S Military HIV Vaccine Research Program, Walter Reed Army Institute of Research, Rockville, MD 20350, USA
    Vaccine 26:3036-45. 2008
    ....
  15. pmc Correlation between lethal toxin-neutralizing antibody titers and protection from intranasal challenge with Bacillus anthracis Ames strain spores in mice after transcutaneous immunization with recombinant anthrax protective antigen
    Kristina K Peachman
    Department of Vaccine Production and Delivery, Division of Retrovirology, U S Military HIV Research Program, Walter Reed Army Institute of Research, 1600 East Gude Drive, Rockville, MD 20850, USA
    Infect Immun 74:794-7. 2006
    ..Future experiments with rabbits and nonhuman primates should confirm the significance of protection by this vaccine strategy...
  16. doi request reprint Liposomes containing glucosyl ceramide specifically bind T4 bacteriophage: a self-assembling nanocarrier formulation
    Carl R Alving
    Department of Adjuvant and Antigen Research, U S Military HIV Research Program, Division of Retrovirology, Walter Reed Army Institute of Research, Rockville, Maryland, USA
    J Liposome Res 21:279-85. 2011
    ..This formulation could be utilized as an easily manufactured self-assembling antigen and adjuvant carrier...
  17. doi request reprint Highly effective generic adjuvant systems for orphan or poverty-related vaccines
    Mangala Rao
    Division of Retrovirology, U S Military HIV Research Program, Walter Reed Army Institute of Research, 1600 East Gude Drive, Rockville, MD 20850, USA
    Vaccine 29:873-7. 2011
    ..All vaccinated monkeys were protected against lethal challenge with aerosolized Ames strain spores...
  18. ncbi request reprint HIV-1, lipid rafts, and antibodies to liposomes: implications for anti-viral-neutralizing antibodies
    Carl R Alving
    Department of Vaccine Production and Delivery, Division of Retrovirology, US Military HIV Research Program, Walter Reed Army Institute of Research, Rockville, MD 20850, USA
    Mol Membr Biol 23:453-65. 2006
    ..The possibility is proposed that liposomal lipids, or liposome-protein combinations, could be useful as antigens for inducing broadly neutralizing antibodies to HIV-1...
  19. ncbi request reprint Restricted replication and lysosomal trafficking of yellow fever 17D vaccine virus in human dendritic cells
    Dupeh R Palmer
    Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, MD, USA
    J Gen Virol 88:148-56. 2007
    ..These results indicate that restricted virus replication and lysosomal compartmentalization may be important contributing factors to the success of the YF-VAX vaccine...
  20. pmc Human erythrocytes selectively bind and enrich infectious HIV-1 virions
    Zoltan Beck
    Division of Retrovirology, United States Military HIV Research Program, Walter Reed Army Institute of Research, Rockville, Maryland, United States of America
    PLoS ONE 4:e8297. 2009
    ..By analogy with HIV-1 bound to DC-SIGN on dendritic cells, erythrocyte-bound HIV-1 might comprise an important surface reservoir for trans infection of permissive cells...
  21. ncbi request reprint Immunostimulatory CpG motifs induce CTL responses to HIV type I oligomeric gp140 envelope protein
    Mangala Rao
    Department of Membrane Biochemistry, Walter Reed Army Institute of Research, Silver Spring, MD 20910 7500, USA
    Immunol Cell Biol 82:523-30. 2004
    ..These results can have significant implications in directing the Th1 or Th2 differentiation of antigen-specific immune responses in the context of vaccine development...
  22. doi request reprint Liposomes containing lipid A: an effective, safe, generic adjuvant system for synthetic vaccines
    Carl R Alving
    Laboratory of Adjuvant and Antigen Research, US Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA
    Expert Rev Vaccines 11:733-44. 2012
    ..They are easily manufactured as potentially inexpensive and easy to use adjuvant systems and might be effective even with synthetic peptides as antigens...
  23. doi request reprint An anti-phosphoinositide-specific monoclonal antibody that neutralizes HIV-1 infection of human monocyte-derived macrophages
    Ousman Jobe
    Laboratory of Adjuvant and Antigen Research, US Military HIV Research Program, Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA
    Virology 430:110-9. 2012
    ..WR321 did not prevent the entry of HIV-1 into MDM. However, once WR321 was internalized along with HIV-1 the mAb acted intracellulary to prevent the release of virions from MDM and also triggered the release of β-chemokines...
  24. ncbi request reprint Trafficking of liposomal antigens to the trans-Golgi complex in macrophages
    Mangala Rao
    Department of Membrane Biochemistry, Walter Reed Army Institute of Research, Washington, D C 20307, USA
    Methods Enzymol 373:16-33. 2003
  25. pmc Adjuvants for human vaccines
    Carl R Alving
    Laboratory of Adjuvant and Antigen Research, U S Military HIV Research Program, Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA
    Curr Opin Immunol 24:310-5. 2012
    ....
  26. ncbi request reprint Depletion of cellular cholesterol interferes with intracellular trafficking of liposome-encapsulated ovalbumin
    Mangala Rao
    Departments of Membrane Biochemistry and Blood Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA
    Immunol Cell Biol 81:415-23. 2003
    ..We conclude that cholesterol depletion does not eliminate phagocytosis or MHC class I surface expression, but does affect the trafficking and consequently the MHC class I antigen-processing pathway...
  27. ncbi request reprint Detection of antibodies to squalene: III. Naturally occurring antibodies to squalene in humans and mice
    Gary R Matyas
    Department of Membrane Biochemistry, Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD 20910 7500, USA
    J Immunol Methods 286:47-67. 2004
    ..7%) and males (12.5%). We conclude that antibodies to SQE occur naturally in humans; have an increased prevalence in females; are not correlated with vaccination with AVA; and appear to increase in prevalence with age...
  28. ncbi request reprint Modulation of dengue virus infection of dendritic cells by Aedes aegypti saliva
    Daniel B Ader
    Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland 20810, USA
    Viral Immunol 17:252-65. 2004
    ..These data contribute to the overall understanding of the natural pathogenesis of DV infection and suggest that there is a protective role for mosquito saliva that limits viral uptake by DCs...
  29. ncbi request reprint Immunization with DNA through the skin
    Kristina K Peachman
    Department of Membrane Biochemistry, Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Room 2A24, Silver Spring, MD 20910 7500, USA
    Methods 31:232-42. 2003
    ..With refinement, skin-DNA immunization will achieve the goal of producing a reliable and efficacious immune response to a variety of pathogens...