Jeffrey R Currier
Affiliation: Division of Retrovirology
- A panel of MHC class I restricted viral peptides for use as a quality control for vaccine trial ELISPOT assaysJeffrey R Currier
The US Military HIV Research Program, Suite 200, 13 Taft Court, Rockville, MD 20851, USA
J Immunol Methods 260:157-72. 2002..The size, shape and appearance of the spots produced using this peptide panel provided a standard for the establishment of acceptance criteria of spots for the evaluation of ELISPOT plates using an automated reader system...
- Defining epitope coverage requirements for T cell-based HIV vaccines: theoretical considerations and practical applicationsJeffrey R Currier
US Military HIV Research Program MHRP, Rockville, MD, USA
J Transl Med 9:212. 2011..Assessment of potential HIV strain coverage by candidate T cell-based vaccines (whether natural sequence or computationally optimized products) is now a critical component in interpreting candidate vaccine suitability...
- Peptide impurities in commercial synthetic peptides and their implications for vaccine trial assessmentJeffrey R Currier
U S Military HIV Research Program, Rockville, Maryland 20850, USA
Clin Vaccine Immunol 15:267-76. 2008..We propose a simple schema of biological QA/QC protocols to augment the standard biochemical QA/QC analyses as a means to circumvent this and other problems that can affect cellular immune-based assay outcome and interpretation...
- Phase I safety and immunogenicity evaluation of MVA-CMDR, a multigenic, recombinant modified vaccinia Ankara-HIV-1 vaccine candidateJeffrey R Currier
United States Military HIV Research Program, Rockville, Maryland, United States of America
PLoS ONE 5:e13983. 2010..The HIV sequences were derived from circulating recombinant form CRF01_AE, which predominates in Thailand. The objective was to evaluate safety and immunogenicity of MVA-CMDR in human volunteers in the US and Thailand...
- CTL epitope distribution patterns in the Gag and Nef proteins of HIV-1 from subtype A infected subjects in Kenya: use of multiple peptide sets increases the detectable breadth of the CTL responseJeffrey R Currier
The US Military HIV Research Program, Rockville, MD 20850, USA
BMC Immunol 7:8. 2006..While considerable effort has been focused upon mapping and defining immunodominant CTL epitopes in HIV-1 subtype B and subtype C infections, few epitope mapping studies have focused upon subtype A...
- Immunodominance and cross-reactivity of B5703-restricted CD8 T lymphocytes from HIV type 1 subtype C-infected EthiopiansJeffrey R Currier
The U S Military HIV Research Program, Rockville, Maryland 20850, USA
AIDS Res Hum Retroviruses 21:239-45. 2005..Efforts to optimize the cross-reactivity of vaccine-induced CD8 T cells may need to focus on the relative immunogenicity of minor sequence variation...
- Detection of high frequencies of HIV-1 cross-subtype reactive CD8 T lymphocytes in the peripheral blood of HIV-1-infected KenyansJeffrey R Currier
US Military HIV Research Program, Suite 200, 13 Taft Court, Rockville, MD 20850, USA
AIDS 17:2149-57. 2003..To quantitate rapidly the frequency of HIV-1 subtype-specific and broadly HIV-1 cross-subtype-reactive CD8 T cells in the peripheral blood of HIV-1-infected individuals from a geographical region of multiple subtype endemicity...
- Comprehensive screening for human immunodeficiency virus type 1 subtype-specific CD8 cytotoxic T lymphocytes and definition of degenerate epitopes restricted by HLA-A0207 and -C(W)0304 allelesJeffrey R Currier
U S Military HIV Research Program, Rockville, Maryland 20850, USA
J Virol 76:4971-86. 2002..These findings provide biological validation of HLA supertypes in HIV-1 CTL recognition and support earlier studies of cross-subtype CTL responses during HIV-1 infection...
- HIV-1 envelope resistance to proteasomal cleavage: implications for vaccine induced immune responsesNicholas J Steers
United States Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America
PLoS ONE 7:e42579. 2012..Preliminary immunological data from the RV144 phase III trial indicated that the immune responses were biased towards the Env antigen with a dominant CD4+ T-cell response...
- Heterologous prime-boost regimens using rAd35 and rMVA vectors elicit stronger cellular immune responses to HIV proteins than homologous regimensSilvia Ratto-Kim
United States Military HIV Research Program, Walter Reed Army Institute of Research, Rockville, Maryland, USA
PLoS ONE 7:e45840. 2012..This study supports the rationale for testing heterologous prime-boost regimens in humans...
- Designing the epitope flanking regions for optimal generation of CTL epitopesNicholas J Steers
United States Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA Henry M Jackson Foundation for the Advancement of Military Medicine, Rockledge, MD 20852, USA
Vaccine 32:3509-16. 2014..These results highlight the importance of flanking regions in promoting efficient antigen processing and presentation. This concept can have important implications in vaccine design and development strategies. ..
- A double-blind randomized phase I clinical trial targeting ALVAC-HIV vaccine to human dendritic cellsMichael A Eller
U S Military HIV Research Program, Rockville, Maryland, United States of America
PLoS ONE 6:e24254. 2011..We explored the concept that direct ex vivo targeting of human dendritic cells (DC) would enhance the immune response compared to either conventional intramuscular or intradermal injections of the vaccine alone...
- Single-cell level response of HIV-specific and cytomegalovirus-specific CD4 T cells correlate with viral control in chronic HIV-1 subtype A infectionMichael A Eller
U S Military HIV Research Program, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA
J Acquir Immune Defic Syndr 61:9-18. 2012..To study the possible role of T cells associated with better outcome, we examined CD4 and CD8 T-cell responses against HIV-1 and cytomegalovirus (CMV) in Ugandans infected with subtype A HIV-1...
- Lost in translation: implications of HIV-1 codon usage for immune escape and drug resistanceGustavo H Kijak
Henry M Jackson Foundation for the Advancement of Military Medicine, 1600 East Gude Drive, Rockville, MD 20850, USA
AIDS Rev 6:54-60. 2004..Quasi-synonymy conditions HIV-1 and, potentially, other rapidly evolving organisms in their exploration of the mutational space...
- HIV-1 MN Env 15-mer peptides better detect HIV-1 specific CD8 T cell responses compared with consensus subtypes B and M group 15-mer peptidesAlleluiah Rutebemberwa
Henry M Jackson Foundation and the US Military HIV Research Program, Rockville, Maryland 20850, USA
AIDS 19:1165-72. 2005..To compare the ability of three Env (15-mer) peptide sets derived from the HIV-1 MN, the subtype B consensus, and the group M consensus to detect HIV-1 specific interferon (IFN)-gamma responses in HIV-1 subtype B infected subjects...
- Preparation of clinical-grade recombinant canarypox-human immunodeficiency virus vaccine-loaded human dendritic cellsMary A Marovich
Division of Retrovirology, US Military HIV Research Program, Rockville, Maryland 20850, USA
J Infect Dis 186:1242-52. 2002..These data support an ongoing HIV vaccine trial comparing conventional vaccine delivery routes with ex vivo vaccine-loaded autologous DCs for immunogenicity in HIV-1-uninfected volunteers...
- Cell type-specific proteasomal processing of HIV-1 Gag-p24 results in an altered epitope repertoireNicholas J Steers
Henry Jackson Foundation for the Advancement of Military Medicine, 1600 East Gude Drive, Rockville, Maryland 20850, USA
J Virol 85:1541-53. 2011..These epitopes have been linked to HIV-1 disease progression. Our results suggest that the source of generation of precursor MHC class I epitopes may be a critical factor for the induction of relevant epitope-specific cytotoxic T cells...
- High-throughput high-resolution class I HLA genotyping in East AfricaRebecca N Koehler
United States Military HIV Research Program Henry M Jackson Foundation, Rockville, Maryland, USA
PLoS ONE 5:e10751. 2010..The availability of genotyping tools with this capacity will be extremely useful in the identification of correlates of immune protection and the evaluation of candidate vaccine efficacy...
- Human immunodeficiency virus type 1 infection is associated with increased NK cell polyfunctionality and higher levels of KIR3DL1+ NK cells in ugandans carrying the HLA-B Bw4 motifMichael A Eller
Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, 14186 Stockholm, Sweden
J Virol 85:4802-11. 2011..These results indicate that chronic HIV-1 infection is associated with increased NK cell polyfunctionality and elevated levels of KIR3DL1(+) NK cells in Ugandans carrying the HLA-B Bw4 motif...
- HLA-A and -B allele expression and ability to develop anti-Gag cross-clade responses in subtype C HIV-1-infected EthiopiansGuido Ferrari
Duke University, Durham, NC, USA
Hum Immunol 65:648-59. 2004..These data represent the first report of correlating HLA phenotype and HIV-specific cell-mediated immune responses among infected Ethiopians and may be useful in designing cytotoxic T lymphocyte-inducing vaccines for this part of Africa...
- CD8 T-cell recognition of multiple epitopes within specific Gag regions is associated with maintenance of a low steady-state viremia in human immunodeficiency virus type 1-seropositive patientsChristof Geldmacher
Vaccine Research Center, National Institute of Allergy and Infectious Diseases NIH, Bethesda, MD 20892, USA
J Virol 81:2440-8. 2007..36; P = 0.0016). Particularly, recognition of multiple epitopes within two regions of Gag (amino acids [aa] 1 to 75 and aa 248 to 500) was associated with the maintenance of a low steady-state viremia, even years after acute infection...
- In a mixed subtype epidemic, the HIV-1 Gag-specific T-cell response is biased towards the infecting subtypeChristof Geldmacher
Mbeya Medical Research Program, Referral Hospital, Mbeya, Tanzania
AIDS 21:135-43. 2007..This study was designed to assess whether the Gag- and Nef-specific T-cell response is biased towards recognizing the infecting subtype...
- A high viral burden predicts the loss of CD8 T-cell responses specific for subdominant gag epitopes during chronic human immunodeficiency virus infectionChristof Geldmacher
Mbeya Medical Research Programme, Referral Hospital, Mbeya, Tanzania
J Virol 81:13809-15. 2007..0001) and subdominant in the hierarchy of Gag-specific responses. The present study indicates that chronic exposure of the human immune system to high levels of HIV viremia is a determinant of virus-specific CD8 T-cell loss...
- Circulating and unique recombinant forms of HIV type 1 containing subsubtype A2Unchalee Visawapoka
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand
AIDS Res Hum Retroviruses 22:695-702. 2006..Monitoring of A2-containing recombinants and subtype C strains, both relatively rare in Kenya, may be informative in the course of cohort development and evaluation of candidate vaccines...