CHARLES ROBERT WIRA

Summary

Affiliation: Dartmouth Medical School
Country: USA

Publications

  1. pmc The innate immune system: gatekeeper to the female reproductive tract
    Charles R Wira
    Department of Physiology, Dartmouth Medical School, One Medical Center Drive, Lebanon, New Hampshire 03756, USA
    Immunology 111:13-5. 2004
  2. ncbi request reprint Epithelial cells in the female reproductive tract: a central role as sentinels of immune protection
    Charles R Wira
    Department of Physiology, Dartmouth Medical School, Lebanon, NH, USA
    Am J Reprod Immunol 53:65-76. 2005
  3. doi request reprint Innate immunity in the human female reproductive tract: endocrine regulation of endogenous antimicrobial protection against HIV and other sexually transmitted infections
    Charles R Wira
    Department of Physiology and Neurobiology, Dartmouth Medical School, Lebanon, NH 03756, USA
    Am J Reprod Immunol 65:196-211. 2011
  4. doi request reprint Sex hormone regulation of innate immunity in the female reproductive tract: the role of epithelial cells in balancing reproductive potential with protection against sexually transmitted pathogens
    Charles R Wira
    Department of Physiology, Dartmouth Medical School, One Medical Center Drive, Lebanon, NH 03756, USA
    Am J Reprod Immunol 63:544-65. 2010
  5. pmc Oestradiol regulation of antigen presentation by uterine stromal cells: role of transforming growth factor-beta production by epithelial cells in mediating antigen-presenting cell function
    Charles R Wira
    Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756 0001, USA
    Immunology 109:398-406. 2003
  6. pmc Epithelial cell secretions from the human female reproductive tract inhibit sexually transmitted pathogens and Candida albicans but not Lactobacillus
    C R Wira
    Department of Physiology and Neurobiology, Dartmouth Medical School, Lebanon, New Hampshire, USA
    Mucosal Immunol 4:335-42. 2011
  7. ncbi request reprint Polarized uterine epithelial cells preferentially present antigen at the basolateral surface: role of stromal cells in regulating class II-mediated epithelial cell antigen presentation
    Charles R Wira
    Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756 0001, USA
    J Immunol 175:1795-804. 2005
  8. ncbi request reprint Innate and adaptive immunity in female genital tract: cellular responses and interactions
    Charles R Wira
    Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756, USA
    Immunol Rev 206:306-35. 2005
  9. ncbi request reprint Influence of stage of the reproductive cycle and estradiol on thymus cell antigen presentation
    Charles R Wira
    Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756, USA
    J Steroid Biochem Mol Biol 84:79-87. 2003
  10. ncbi request reprint Antigen presentation by vaginal cells: role of TGFbeta as a mediator of estradiol inhibition of antigen presentation
    Charles R Wira
    Department of Physiology, Dartmouth Medical School, Borwell Building, 1 Medical Center Drive, Lebanon, NH 03756, USA
    Endocrinology 143:2872-9. 2002

Collaborators

Detail Information

Publications63

  1. pmc The innate immune system: gatekeeper to the female reproductive tract
    Charles R Wira
    Department of Physiology, Dartmouth Medical School, One Medical Center Drive, Lebanon, New Hampshire 03756, USA
    Immunology 111:13-5. 2004
  2. ncbi request reprint Epithelial cells in the female reproductive tract: a central role as sentinels of immune protection
    Charles R Wira
    Department of Physiology, Dartmouth Medical School, Lebanon, NH, USA
    Am J Reprod Immunol 53:65-76. 2005
    ..In this article, we will outline how polarized epithelial cells function as the first line of defense against potential pathogens in the female reproductive tract...
  3. doi request reprint Innate immunity in the human female reproductive tract: endocrine regulation of endogenous antimicrobial protection against HIV and other sexually transmitted infections
    Charles R Wira
    Department of Physiology and Neurobiology, Dartmouth Medical School, Lebanon, NH 03756, USA
    Am J Reprod Immunol 65:196-211. 2011
    ..The goal of this review is to examine the spectrum of antimicrobials in the FRT and the ways in which they are regulated to provide protection against pathogens that compromise reproductive health and threaten the lives of women...
  4. doi request reprint Sex hormone regulation of innate immunity in the female reproductive tract: the role of epithelial cells in balancing reproductive potential with protection against sexually transmitted pathogens
    Charles R Wira
    Department of Physiology, Dartmouth Medical School, One Medical Center Drive, Lebanon, NH 03756, USA
    Am J Reprod Immunol 63:544-65. 2010
    ..In doing so, a window of STI vulnerability is created during which potential pathogens including HIV enter the reproductive tract to infect host targets...
  5. pmc Oestradiol regulation of antigen presentation by uterine stromal cells: role of transforming growth factor-beta production by epithelial cells in mediating antigen-presenting cell function
    Charles R Wira
    Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756 0001, USA
    Immunology 109:398-406. 2003
    ..Taken together, these results suggest that oestradiol inhibition of stromal cell antigen presentation is mediated through the stimulatory effect of oestradiol on TGF-beta production by epithelial cells...
  6. pmc Epithelial cell secretions from the human female reproductive tract inhibit sexually transmitted pathogens and Candida albicans but not Lactobacillus
    C R Wira
    Department of Physiology and Neurobiology, Dartmouth Medical School, Lebanon, New Hampshire, USA
    Mucosal Immunol 4:335-42. 2011
    ..crispatus, which is part of the normal vaginal microflora of humans...
  7. ncbi request reprint Polarized uterine epithelial cells preferentially present antigen at the basolateral surface: role of stromal cells in regulating class II-mediated epithelial cell antigen presentation
    Charles R Wira
    Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756 0001, USA
    J Immunol 175:1795-804. 2005
    ..Overall, these results demonstrate that polarized epithelial cells are able to present Ags and suggest that uterine stromal cells communicate with epithelial cells via a soluble factor(s) to regulate Ag presentation in the uterus...
  8. ncbi request reprint Innate and adaptive immunity in female genital tract: cellular responses and interactions
    Charles R Wira
    Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756, USA
    Immunol Rev 206:306-35. 2005
    ..By understanding the nature of this protection and the ways in which innate and adaptive immunity interact, these studies provide the opportunity to contribute to the foundation of information essential for ensuring reproductive health...
  9. ncbi request reprint Influence of stage of the reproductive cycle and estradiol on thymus cell antigen presentation
    Charles R Wira
    Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756, USA
    J Steroid Biochem Mol Biol 84:79-87. 2003
    ..1 and B7.2. These studies demonstrate that sex hormones play a central role in regulating antigen presentation in the thymus...
  10. ncbi request reprint Antigen presentation by vaginal cells: role of TGFbeta as a mediator of estradiol inhibition of antigen presentation
    Charles R Wira
    Department of Physiology, Dartmouth Medical School, Borwell Building, 1 Medical Center Drive, Lebanon, NH 03756, USA
    Endocrinology 143:2872-9. 2002
    ..These studies suggest that estradiol regulation of antigen presentation by vaginal cells is mediated through the local production of TGFbeta by vaginal cells...
  11. ncbi request reprint Sex hormone regulation of anti-bacterial activity in rat uterine secretions and apical release of anti-bacterial factor(s) by uterine epithelial cells in culture
    John V Fahey
    Department of Physiology, Borwell Building, Dartmouth Medical School, One Medical Center Drive, Lebanon, NH 03756, USA
    J Steroid Biochem Mol Biol 93:59-66. 2005
    ..Further these studies suggest that epithelial cells which line the uterine lumen are a primary source of anti-bacterial activity...
  12. ncbi request reprint Hepatocyte growth factor regulation of uterine epithelial cell transepithelial resistance and tumor necrosis factor alpha release in culture
    Katherine S Grant-Tschudy
    Department of Physiology, Dartmouth Medical School, Lebanon, New Hampshire 03756, USA
    Biol Reprod 72:814-21. 2005
    ..Furthermore, because neutralization of stromal media failed to affect TNFalpha secretion, these findings suggest that other growth factors, in addition to HGF, affect EC cytokine production...
  13. pmc Effect of oestradiol on mouse uterine epithelial cell tumour necrosis factor-alpha release is mediated through uterine stromal cells
    Katherine S Grant-Tschudy
    Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756 0001, USA
    Immunology 115:99-107. 2005
    ....
  14. ncbi request reprint Unique characteristics of NK cells throughout the human female reproductive tract
    Teddy F Mselle
    Department of Microbiology and Immunology, Dartmouth Medical School, One Medical Center Drive, Lebanon, NH 03756, USA
    Clin Immunol 124:69-76. 2007
    ..These data suggest that unique characteristics of the tissues may account of specific localization of different NK cell subsets...
  15. pmc Chemokine receptor expression in the human ectocervix: implications for infection by the human immunodeficiency virus-type I
    Grant R Yeaman
    Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, NH, USA
    Immunology 113:524-33. 2004
    ..These findings are important to define potential targets of HIV-1 infection within the FRT, and for the future design of approaches to reduce the susceptibility of women to infection by HIV-1...
  16. doi request reprint CCL20/MIP3alpha is a novel anti-HIV-1 molecule of the human female reproductive tract
    Mimi Ghosh
    Department of Physiology, Dartmouth Medical School, Lebanon, NH, USA
    Am J Reprod Immunol 62:60-71. 2009
    ..The role of CCL20/MIP3alpha as an antiviral is unknown. In this study, we have examined the production of CCL20/MIP3alpha by epithelial cells from the upper female reproductive tract as well as its activity as an antiviral molecule...
  17. ncbi request reprint Estradiol attenuates lipopolysaccharide-induced CXC chemokine ligand 8 production by human peripheral blood monocytes
    Patricia A Pioli
    Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756, USA
    J Immunol 179:6284-90. 2007
    ..These results implicate a role for estradiol in the modulation of the immune response, and may lead to an enhanced understanding of gender-based differences in inflammatory control mechanisms...
  18. pmc Effect of oestradiol on PAMP-mediated CCL20/MIP-3 alpha production by mouse uterine epithelial cells in culture
    Gisela Soboll
    Department of Physiology, Dartmouth Medical School, Lebanon, NH 03765, USA
    Immunology 118:185-94. 2006
    ....
  19. pmc Human uterine epithelial cell secretions regulate dendritic cell differentiation and responses to TLR ligands
    Daniel O Ochiel
    Department of Physiology, Dartmouth Medical School, One Medical Center Drive, Lebanon, NH 03756, USA
    J Leukoc Biol 88:435-44. 2010
    ..These findings indicate collectively that epithelial cells confer a tolerogenic phenotype to DC in the endometrium. Our results suggest novel cellular and molecular mechanisms for the regulation of adaptive immunity within the FRT...
  20. pmc Human uterine NK cells interact with uterine macrophages via NKG2D upon stimulation with PAMPs
    Satarupa Basu
    Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, NH 03756, USA
    Am J Reprod Immunol 61:52-61. 2009
    ..In the human endometrium, uterine natural killer (uNK) cells and uterine macrophages are present in significant numbers and in close proximity, yet how they cooperatively respond to infectious challenge is poorly understood...
  21. pmc Estradiol regulates expression of estrogen receptor ERalpha46 in human macrophages
    Amy J Murphy
    Department of Physiology, Dartmouth Medical School, Lebanon, New Hampshire, United States of America
    PLoS ONE 4:e5539. 2009
    ..However, relatively little is known about the ability of E2 to regulate isoform expression of estrogen receptors (ERs) in these cells...
  22. pmc Functional expression of pattern recognition receptors in tissues of the human female reproductive tract
    Kevin M Hart
    Department of Immunology and Microbiology, Dartmouth Medical School, Lebanon, NH 03756, USA
    J Reprod Immunol 80:33-40. 2009
    ..Results of these studies indicate that PRRs are functionally expressed in FRT tissues, and suggest that these receptors mediate microbial recognition and immune defense in the reproductive tract...
  23. ncbi request reprint Effect of toll-like receptor (TLR) agonists on TLR and microbicide expression in uterine and vaginal tissues of the mouse
    Gisela Soboll
    Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756, USA
    Am J Reprod Immunol 55:434-46. 2006
    ....
  24. ncbi request reprint Expression of Toll-like receptors (TLR) and responsiveness to TLR agonists by polarized mouse uterine epithelial cells in culture
    Gisela Soboll
    Department of Physiology, Dartmouth Medical School, Lebanon, New Hampshire 03756, USA
    Biol Reprod 75:131-9. 2006
    ....
  25. ncbi request reprint Unique phenotype of human uterine NK cells and their regulation by endogenous TGF-beta
    Mikael Eriksson
    Dartmouth Medical School, One Medical Center Drive, Lebanon, NH 03756, USA
    J Leukoc Biol 76:667-75. 2004
    ..Further, data demonstrate that uNK cells can produce immunoregulatory cytokines and that inhibition of uNK cells by locally produced TGF-beta1 is a likely mechanism to regulate NK cell function in the human EM...
  26. pmc Trappin-2/Elafin: a novel innate anti-human immunodeficiency virus-1 molecule of the human female reproductive tract
    Mimi Ghosh
    Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756, USA
    Immunology 129:207-19. 2010
    ..Our data suggest that Trappin-2/Elafin might be an important endogenous microbicide of the female reproductive tract that is protective against HIV-1...
  27. pmc Uterine epithelial cell regulation of DC-SIGN expression inhibits transmitted/founder HIV-1 trans infection by immature dendritic cells
    Daniel O Ochiel
    Department of Physiology, Dartmouth Medical School, Lebanon, New Hampshire, United States of America
    PLoS ONE 5:e14306. 2010
    ..We hypothesized in this study that specific immune modulators secreted by uterine epithelial cells modulate susceptibility of dendritic cells (DC) to infection with HIV-1...
  28. doi request reprint New approaches to making the microenvironment of the female reproductive tract hostile to HIV
    John V Fahey
    Department of Physiology and Neurobiology, Dartmouth Medical School, One Medical Center Drive, Lebanon, NH 03756, USA
    Am J Reprod Immunol 65:334-43. 2011
    ..all of which are under hormonal control, will open new avenues to therapeutic manipulation of the FRT mucosal microenvironment. By seeking new alternatives to preventing HIV infection in women, we may finally disrupt the HIV pandemic...
  29. ncbi request reprint NK cell function in the human female reproductive tract
    Charles L Sentman
    Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, NH 03756, USA
    Am J Reprod Immunol 57:108-15. 2007
    ..Uterine NK cells have a unique phenotype compared with blood NK cell subsets, yet little is known about how NK cells function as a part of the innate immune cell network in the female reproductive tract...
  30. pmc Human uterine natural killer cells but not blood natural killer cells inhibit human immunodeficiency virus type 1 infection by secretion of CXCL12
    Teddy F Mselle
    Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, New Hampshire 03756, USA
    J Virol 83:11188-95. 2009
    ....
  31. ncbi request reprint Lipopolysaccharide-induced IL-1 beta production by human uterine macrophages up-regulates uterine epithelial cell expression of human beta-defensin 2
    Patricia A Pioli
    Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756, USA
    J Immunol 176:6647-55. 2006
    ..These data indicate dynamic immunologic interaction between uterine macrophages and epithelial cells and implicate a role for estradiol in the modulation of the immune response...
  32. doi request reprint Estradiol modulation of hepatocyte growth factor by stromal fibroblasts in the female reproductive tract
    Kimberly D Coleman
    Department of Physiology, Dartmouth Medical School, One Medical Center Drive, Lebanon, NH 03756, USA
    Fertil Steril 92:1107-9. 2009
    ..Estradiol-induced HGF secretion by uterine stromal fibroblasts may have a significant effect on uterine cancer and endometriosis...
  33. pmc Antiviral responses of human Fallopian tube epithelial cells to toll-like receptor 3 agonist poly(I:C)
    Mimi Ghosh
    Department of Physiology, Dartmouth Medical School, Lebanon, New Hampshire 03756, USA
    Fertil Steril 89:1497-506. 2008
    ..To examine the expression of toll-like receptors (TLR) by primary human Fallopian tube epithelial cells (FTEC) and to determine whether exposure to the TLR3 agonist poly(I:C) induces an antiviral response...
  34. pmc CCL20/macrophage inflammatory protein 3alpha and tumor necrosis factor alpha production by primary uterine epithelial cells in response to treatment with lipopolysaccharide or Pam3Cys
    Mardi A Crane-Godreau
    Department of Physiology, Dartmouth Medical School, Borwell Building, 1 Medical Center Dr, Lebanon, NH 03756, USA
    Infect Immun 73:476-84. 2005
    ..Since CCL20 is known to be both chemotactic and antimicrobial, the increase in apical and basolateral release by UEC in response to PAMPs suggests a new mechanism of innate immune protection in the female reproductive tract...
  35. pmc Effect of Escherichia coli and Lactobacillus rhamnosus on macrophage inflammatory protein 3 alpha, tumor necrosis factor alpha, and transforming growth factor beta release by polarized rat uterine epithelial cells in culture
    Mardi A Crane-Godreau
    Department of Physiology, Dartmouth Medical School, Lebanon, New Hampshire, 03756 0001, USA
    Infect Immun 72:1866-73. 2004
    ..These findings suggest that UEC responses to bacteria are selective and important in initiating and regulating immune protection in the female reproductive tract...
  36. pmc Effects of estradiol on lipopolysaccharide and Pam3Cys stimulation of CCL20/macrophage inflammatory protein 3 alpha and tumor necrosis factor alpha production by uterine epithelial cells in culture
    Mardi A Crane-Godreau
    Department of Physiology, Dartmouth Medical School, Borwell Building, 1 Medical Center Drive, Lebanon, New Hampshire 03756, USA
    Infect Immun 73:4231-7. 2005
    ....
  37. ncbi request reprint Innate immunity in the human female reproductive tract: antiviral response of uterine epithelial cells to the TLR3 agonist poly(I:C)
    Todd M Schaefer
    Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756, USA
    J Immunol 174:992-1002. 2005
    ....
  38. pmc Toll-like receptor (TLR) expression and TLR-mediated cytokine/chemokine production by human uterine epithelial cells
    Todd M Schaefer
    Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756, USA
    Immunology 112:428-36. 2004
    ....
  39. ncbi request reprint Recruitment of uterine NK cells: induction of CXC chemokine ligands 10 and 11 in human endometrium by estradiol and progesterone
    Charles L Sentman
    Department of, Microbiology and Immunology, Dartmouth Medical School, Lebanon, NH 03756, USA
    J Immunol 173:6760-6. 2004
    ....
  40. ncbi request reprint Synergy between IL-8 and GM-CSF in reproductive tract epithelial cell secretions promotes enhanced neutrophil chemotaxis
    Li Shen
    Department of Immunology and Microbiology, Dartmouth Medical School, Dartmouth Hitchcock Medical Center, 1 Medical Center Drive, Lebanon, NH 03756, USA
    Cell Immunol 230:23-32. 2004
    ..These findings demonstrate that the majority of neutrophil chemoattractant activity produced by FRT EC results from the synergistic effects of IL-8 and GM-CSF...
  41. ncbi request reprint Effect of estradiol on mouse uterine epithelial cell transepithelial resistance (TER)
    Katherine S Grant-Tschudy
    Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756 0001, USA
    Am J Reprod Immunol 52:252-62. 2004
    ..The aim of this study was to test whether estradiol-17beta (E(2)) acts directly to regulate uterine epithelial cell monolayer integrity...
  42. pmc Differential expression of Toll-like receptors 2 and 4 in tissues of the human female reproductive tract
    Patricia A Pioli
    Department of Physiology, Dartmouth Medical School, Lebanon, New Hampshire 03756, USA
    Infect Immun 72:5799-806. 2004
    ..These data suggest that TLRs are differentially expressed in distinct compartments of the female reproductive tract and may provide insight regarding the regulation of inflammation and immunity within the tract...
  43. ncbi request reprint Secretion of monocyte chemotactic protein-1 by human uterine epithelium directs monocyte migration in culture
    Richard A Meter
    Department of Obstetrics and Gynecology, Dartmouth Hitchcock Medical Center, Lebanon, New Hampshire 03756, USA
    Fertil Steril 84:191-201. 2005
    ..To determine whether primary human uterine epithelial cells in culture are able to influence monocyte chemotaxis and to establish whether the causal agent of chemotaxis is monocyte chemotactic protein (MCP)-1...
  44. ncbi request reprint Transmission of HIV-1 by primary human uterine epithelial cells and stromal fibroblasts
    Susana N Asin
    Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, New Hampshire, USA
    J Infect Dis 190:236-45. 2004
    ..These data suggest that uterine cells are targets for productive infection with X4-tropic strains and release unmodified R5-tropic viruses that would then be able to infect submucosal target cells, including T cells and macrophages...
  45. ncbi request reprint HIV-1 infection of the female reproductive tract
    Alexandra L Howell
    VA Medical Center, 215 North Main Street, White River Junction, VT 05009, USA
    Curr HIV/AIDS Rep 2:35-8. 2005
    ..This indicates that several mechanisms for viral infection and transmission are present throughout the FRT...
  46. ncbi request reprint Antigen presentation by human uterine epithelial cells to autologous T cells
    John V Fahey
    Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756, USA
    Am J Reprod Immunol 55:1-11. 2006
    ..Our goal in this study was to assess the ability of human uterine epithelial cells to present antigen to cells of the adaptive immune system...
  47. ncbi request reprint Human immunodeficiency virus type 1 infection of human uterine epithelial cells: viral shedding and cell contact-mediated infectivity
    Susana N Asin
    Department of Microbiology, Dartmouth Medical School, Lebanon, New Hampshire, USA
    J Infect Dis 187:1522-33. 2003
    ..These data demonstrated that HIV-1 infects human epithelial cells of upper reproductive tract origin and that productive viral infection of epithelial cells may be an important mechanism of transmission of HIV-1 infection in women...
  48. ncbi request reprint IL-1beta-mediated proinflammatory responses are inhibited by estradiol via down-regulation of IL-1 receptor type I in uterine epithelial cells
    Todd M Schaefer
    Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756, USA
    J Immunol 175:6509-16. 2005
    ....
  49. ncbi request reprint Paracrine mediators of mouse uterine epithelial cell transepithelial resistance in culture
    Katherine S Grant-Tschudy
    Department of Physiology, Dartmouth Medical School, Borwell Building, 1 Medical Center Drive, Lebanon, NH 03756 0001 USA
    J Reprod Immunol 67:1-12. 2005
    ..These studies suggest that factors, such as hormone balance, pathogen exposure as well as pregnancy, which affect cytokine and growth factor secretion, influence epithelial cell barrier protection within the female reproductive tract...
  50. ncbi request reprint Effect of mouse uterine stromal cells on epithelial cell transepithelial resistance (TER) and TNFalpha and TGFbeta release in culture
    Katherine S Grant
    Department of Physiology, Dartmouth Medical School, Lebanon, New Hampshire 03756, USA
    Biol Reprod 69:1091-8. 2003
    ..These results suggest that uterine stromal cells communicate with epithelial cells via a soluble factor(s) to maintain uterine integrity and epithelial secretory function...
  51. ncbi request reprint Migration inhibitory factor secretion by polarized uterine epithelial cells is enhanced in response to the TLR3 agonist poly (I:C)
    Todd M Schaefer
    Department of Physiology, Dartmouth Medical School, 710W Borwell, 1 Medical Center Drive, Lebanon, NH 03756, USA
    Am J Reprod Immunol 54:193-202. 2005
    ..The goals of this study were to determine if uterine epithelial cells produce the pro-inflammatory cytokine macrophage migration inhibitory factor (MIF), and to see if toll-like receptor (TLR) agonists stimulate MIF secretion...
  52. ncbi request reprint Effect of menstrual status on antibacterial activity and secretory leukocyte protease inhibitor production by human uterine epithelial cells in culture
    John V Fahey
    Department of Physiology, Dartmouth Medical School, One Medical Center Drive, Lebanon, New Hampshire 03756, USA
    J Infect Dis 185:1606-13. 2002
    ..These results indicate that menstrual status (pre- vs. postmenopausal) influences the production of SLPI and bactericidal activity by uterine epithelial cells...
  53. pmc Anti-HIV activity in cervical-vaginal secretions from HIV-positive and -negative women correlate with innate antimicrobial levels and IgG antibodies
    Mimi Ghosh
    Department of Physiology, Dartmouth Medical School, Lebanon, New Hampshire, United States of America
    PLoS ONE 5:e11366. 2010
    ..Since female reproductive tract (FRT) secretions contain a spectrum of antimicrobials, we hypothesized that CVL from healthy HIV(+) and (-) women inhibit HIV infection...
  54. ncbi request reprint Inhibition of human polymorphonuclear cell oxidative burst by 17-beta-estradiol and 2,3,7,8-tetrachlorodibenzo-p-dioxin
    Vikki M Abrahams
    Department of Microbiology and Immunology, Dartmouth Medical School, 1 Medical Center Drive, Lebanon, NH 03756, USA
    Am J Reprod Immunol 50:463-72. 2003
    ..This may have significant consequences on PMN function within the female reproductive tract. This study evaluated the effects of 17-beta-estradiol and TCDD on PMN oxidative burst...
  55. pmc Differential regulation of neutrophil chemotaxis to IL-8 and fMLP by GM-CSF: lack of direct effect of oestradiol
    Li Shen
    Department of Immunology and Microbiology, Dartmouth Medical School, Dartmouth Hitchcock Medical Center, Lebanon, NH 03756, USA
    Immunology 117:205-12. 2006
    ..These data suggest that neutrophil function may be altered by cytokines such as GM-CSF through modulation of signalling and independently of surface receptor expression...
  56. ncbi request reprint Endogenous transforming growth factor-beta inhibits toll-like receptor mediated activation of human uterine natural killer cells
    Mikael Eriksson
    Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, NH 03756, USA
    Am J Reprod Immunol 56:321-8. 2006
    ..Our aim was to determine whether uterine natural killer (uNK) cell cytokine production induced by stimulation through TLRs could be regulated by endogenous transforming growth factor (TGF)-beta in human endometrium...
  57. pmc Estradiol regulates MICA expression in human endometrial cells
    Satarupa Basu
    Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, NH 03756, USA
    Clin Immunol 129:325-32. 2008
    ..Thus, estrogens regulate expression of MICA. These data suggest hormonal regulation of innate immunity and NKG2D-mediated recognition in other tissues and diseases where estrogen may be involved...
  58. ncbi request reprint Human fallopian tube neutrophils--a distinct phenotype from blood neutrophils
    Jennifer M Smith
    Department of Microbiology and Immunology, Dartmouth Medical School, 1 Medical Center Drive, Lebanon, NH 03756, USA
    Am J Reprod Immunol 56:218-29. 2006
    ..The role of neutrophils in the human Fallopian tube (FT) is unknown. In order to provide insights into their functions in the FT, we systematically compared neutrophils from normal FT and peripheral blood (PB)...
  59. ncbi request reprint Effects of menstrual cycle status and gender on human neutrophil phenotype
    Jennifer M Smith
    Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, NH 03756, USA
    Am J Reprod Immunol 58:111-9. 2007
    ..The effects of gender and fluctuating ovarian hormones on neutrophil phenotype have yet to be characterized...
  60. ncbi request reprint TLRs mediate IFN-gamma production by human uterine NK cells in endometrium
    Mikael Eriksson
    Department of Microbiology and Immunology, Dartmouth Medical School, One Medical Center Drive, Lebanon, NH 03756, USA
    J Immunol 176:6219-24. 2006
    ..These data also indicate that the uNK cells do not respond directly to TLR stimulation, but rather their production of IFN-gamma is dependent upon interactions with other cells within EM...
  61. ncbi request reprint PRO 2000 elicits a decline in genital tract immune mediators without compromising intrinsic antimicrobial activity
    Marla J Keller
    Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
    AIDS 21:467-76. 2007
    ..The objective of this study was to examine the effect of 14 daily applications of 0.5% PRO 2000 or placebo gel on mediators of mucosal immunity and intrinsic antimicrobial activity...
  62. ncbi request reprint Expression and secretion of antiviral factors by trophoblast cells following stimulation by the TLR-3 agonist, Poly(I : C)
    Vikki M Abrahams
    Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, CT 06520, USA
    Hum Reprod 21:2432-9. 2006
    ..Recent insight into the immunological capabilities of the trophoblast suggests that the placenta may function as an active barrier by recognizing and responding to pathogens through Toll-like receptors (TLRs)...
  63. pmc Human immunodeficiency virus receptor and coreceptor expression on human uterine epithelial cells: regulation of expression during the menstrual cycle and implications for human immunodeficiency virus infection
    Grant R Yeaman
    Department of Obstetrics and Gynecology, Vanderbilt University, Nashville, TN, USA
    Immunology 109:137-46. 2003
    ..In addition, these studies provide evidence that the uterus, and potentially the entire upper reproductive tract, are important sites for the initial events involved in HIV infection...

Research Grants22

  1. SEX HORMONE REGULATION OF THE MUCOSAL IMMUNE SYSTEM
    Charles Wira; Fiscal Year: 2007
    ....
  2. Innate Immune Protection Against HIV-1 by Reproductive Tract Epithelial Cells
    Charles Wira; Fiscal Year: 2007
    ..These studies should provide the basis of knowledge essential for the development of more effective microbicides and therapeutic interventions. ..
  3. Innate Immune Protection Against HIV-1 by Reproductive Tract Epithelial Cells
    Charles Wira; Fiscal Year: 2007
    ..These studies should provide the basis of knowledge essential for the development of more effective microbicides, therapeutic interventions, ..
  4. SEX HORMONE REGULATION OF THE MUCOSAL IMMUNE SYSTEM
    CHARLES ROBERT WIRA; Fiscal Year: 2010
    ....
  5. SEX HORMONE REGULATION OF THE MUCOSAL IMMUNE SYSTEM
    Charles Wira; Fiscal Year: 1993
    ..If SC and/or IgA mRNA is hormonally regulated, then synthesis of message will be analyzed. In situ hybridization studies will be carried out to identify cells in the reproductive tract that synthesize SC and IgA...
  6. Innate Immune Protection Against HIV-1 by Reproductive Tract Epithelial Cells
    CHARLES ROBERT WIRA; Fiscal Year: 2010
    ..These studies should provide the basis of knowledge essential for the development of more effective microbicides and therapeutic interventions. ..