William Wickner

Summary

Affiliation: Dartmouth Medical School
Country: USA

Publications

  1. pmc Yeast vacuoles and membrane fusion pathways
    William Wickner
    Department of Biochemistry, Dartmouth Medical School, 7200 Vail Building, Hanover, NH 03755 3844, USA
    EMBO J 21:1241-7. 2002
  2. pmc Minimal membrane docking requirements revealed by reconstitution of Rab GTPase-dependent membrane fusion from purified components
    Christopher Stroupe
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Proc Natl Acad Sci U S A 106:17626-33. 2009
  3. pmc HOPS initiates vacuole docking by tethering membranes before trans-SNARE complex assembly
    Christopher M Hickey
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Mol Biol Cell 21:2297-305. 2010
  4. pmc Membrane fusion
    William Wickner
    Department of Biochemistry, Dartmouth Medical School, 7200 Vail Building, Hanover, New Hampshire 03755 3844, USA
    Nat Struct Mol Biol 15:658-64. 2008
  5. doi request reprint Membrane fusion: five lipids, four SNAREs, three chaperones, two nucleotides, and a Rab, all dancing in a ring on yeast vacuoles
    William Wickner
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755 3844, USA
    Annu Rev Cell Dev Biol 26:115-36. 2010
  6. ncbi request reprint Protein translocation across biological membranes
    William Wickner
    Department of Biological Chemistry, Dartmouth Medical School, 7200 Vail Building, Hanover, NH 03755 3844, USA
    Science 310:1452-6. 2005
  7. pmc Phosphoinositides and SNARE chaperones synergistically assemble and remodel SNARE complexes for membrane fusion
    Joji Mima
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Proc Natl Acad Sci U S A 106:16191-6. 2009
  8. pmc Reversible, cooperative reactions of yeast vacuole docking
    Youngsoo Jun
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    EMBO J 25:5260-9. 2006
  9. pmc Sec18p and Vam7p remodel trans-SNARE complexes to permit a lipid-anchored R-SNARE to support yeast vacuole fusion
    Youngsoo Jun
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755 3844, USA
    EMBO J 26:4935-45. 2007
  10. pmc Reconstituted membrane fusion requires regulatory lipids, SNAREs and synergistic SNARE chaperones
    Joji Mima
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755 3844, USA
    EMBO J 27:2031-42. 2008

Collaborators

Detail Information

Publications31

  1. pmc Yeast vacuoles and membrane fusion pathways
    William Wickner
    Department of Biochemistry, Dartmouth Medical School, 7200 Vail Building, Hanover, NH 03755 3844, USA
    EMBO J 21:1241-7. 2002
    ..Recent progress is reviewed in the context of general questions in the membrane fusion field...
  2. pmc Minimal membrane docking requirements revealed by reconstitution of Rab GTPase-dependent membrane fusion from purified components
    Christopher Stroupe
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Proc Natl Acad Sci U S A 106:17626-33. 2009
    ..We use this reconstituted system to show that SNAREs and Sec17p/Sec18p, and Ypt7p and the HOPS complex, are required for stable intermembrane interactions and that the three vacuolar Q-SNAREs are sufficient for these interactions...
  3. pmc HOPS initiates vacuole docking by tethering membranes before trans-SNARE complex assembly
    Christopher M Hickey
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Mol Biol Cell 21:2297-305. 2010
    ..SNAREs further stabilize the associations of HOPS-tethered membranes. HOPS then protects newly formed trans-SNARE complexes from disassembly by Sec17p/Sec18p...
  4. pmc Membrane fusion
    William Wickner
    Department of Biochemistry, Dartmouth Medical School, 7200 Vail Building, Hanover, New Hampshire 03755 3844, USA
    Nat Struct Mol Biol 15:658-64. 2008
    ..In contrast, mitochondrial and cell-cell fusion events are regulated by and use distinct catalysts...
  5. doi request reprint Membrane fusion: five lipids, four SNAREs, three chaperones, two nucleotides, and a Rab, all dancing in a ring on yeast vacuoles
    William Wickner
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755 3844, USA
    Annu Rev Cell Dev Biol 26:115-36. 2010
    ....
  6. ncbi request reprint Protein translocation across biological membranes
    William Wickner
    Department of Biological Chemistry, Dartmouth Medical School, 7200 Vail Building, Hanover, NH 03755 3844, USA
    Science 310:1452-6. 2005
    ....
  7. pmc Phosphoinositides and SNARE chaperones synergistically assemble and remodel SNARE complexes for membrane fusion
    Joji Mima
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Proc Natl Acad Sci U S A 106:16191-6. 2009
    ..This ternary synergy of phosphoinositides and 2 SNARE chaperone systems is required for rapid fusion...
  8. pmc Reversible, cooperative reactions of yeast vacuole docking
    Youngsoo Jun
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    EMBO J 25:5260-9. 2006
    ..Docked vacuoles finally assemble SNARE complexes, yet still require physiological temperature and lipid rearrangements to complete fusion...
  9. pmc Sec18p and Vam7p remodel trans-SNARE complexes to permit a lipid-anchored R-SNARE to support yeast vacuole fusion
    Youngsoo Jun
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755 3844, USA
    EMBO J 26:4935-45. 2007
    ....
  10. pmc Reconstituted membrane fusion requires regulatory lipids, SNAREs and synergistic SNARE chaperones
    Joji Mima
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755 3844, USA
    EMBO J 27:2031-42. 2008
    ..This is the first chemically defined model of the physiological interactions of these conserved fusion catalysts...
  11. pmc HOPS proofreads the trans-SNARE complex for yeast vacuole fusion
    Vincent J Starai
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Mol Biol Cell 19:2500-8. 2008
    ..This is the most direct evidence to date that HOPS is directly involved in the fusion event...
  12. ncbi request reprint Distinct targeting and fusion functions of the PX and SNARE domains of yeast vacuolar Vam7p
    Rutilio A Fratti
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    J Biol Chem 282:13133-8. 2007
    ..The PX domain, through its affinities for phosphoinositides and HOPS, is thus exclusively required for enhancing the targeting of Vam7p rather than for execution of the Vam7p functions in HOPS.SNARE complex assembly and fusion...
  13. pmc The major role of the Rab Ypt7p in vacuole fusion is supporting HOPS membrane association
    Christopher M Hickey
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    J Biol Chem 284:16118-25. 2009
    ..Thus, although Ypt7p may contribute to other fusion functions, its central role is to bind HOPS to the membrane...
  14. pmc Hierarchy of protein assembly at the vertex ring domain for yeast vacuole docking and fusion
    Li Wang
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    J Cell Biol 160:365-74. 2003
    ..Our findings provide a unique view of the functional relationships between GTPases, SNAREs, and actin in membrane fusion...
  15. ncbi request reprint Bem1p is a positive regulator of the homotypic fusion of yeast vacuoles
    Hao Xu
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755 3844, USA
    J Biol Chem 281:27158-66. 2006
    ..2005) Genes Dev. 19, 2606-2618), we did not find phosphorylation of Bem1p at Ser-72 to be required for Bem1p-stimulated fusion. Taken together, Bem1p is a positive regulator of lipid mixing during vacuole hemifusion and fusion...
  16. ncbi request reprint Ion regulation of homotypic vacuole fusion in Saccharomyces cerevisiae
    Vincent J Starai
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755 3844, USA
    J Biol Chem 280:16754-62. 2005
    ....
  17. ncbi request reprint Stringent 3Q.1R composition of the SNARE 0-layer can be bypassed for fusion by compensatory SNARE mutation or by lipid bilayer modification
    Rutilio A Fratti
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    J Biol Chem 282:14861-7. 2007
    ..Thus, SNARE function as regulated by the 0-layer is intimately coupled to the lipids, which must rearrange for fusion...
  18. pmc A soluble SNARE drives rapid docking, bypassing ATP and Sec17/18p for vacuole fusion
    Naomi Thorngren
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755 3844, USA
    EMBO J 23:2765-76. 2004
    ....
  19. pmc HOPS prevents the disassembly of trans-SNARE complexes by Sec17p/Sec18p during membrane fusion
    Hao Xu
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH, USA
    EMBO J 29:1948-60. 2010
    ..HOPS thus directs the Sec17p/Sec18p chaperone system to maximize functional trans-SNARE complex for membrane fusion, a new role of tethering factors during membrane traffic...
  20. pmc Interdependent assembly of specific regulatory lipids and membrane fusion proteins into the vertex ring domain of docked vacuoles
    Rutilio A Fratti
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    J Cell Biol 167:1087-98. 2004
    ..Thus, the assembly of the vacuole vertex ring microdomain arises from interdependent lipid and protein partitioning and binding rather than either lipid partitioning or protein interactions alone...
  21. pmc Phosphorylation of the effector complex HOPS by the vacuolar kinase Yck3p confers Rab nucleotide specificity for vacuole docking and fusion
    Michael Zick
    Department of Biochemistry, Geisel School of Medicine at Dartmouth, Hanover, NH 03755 3844, USA
    Mol Biol Cell 23:3429-37. 2012
    ..Thus guanine nucleotide specificity of the vacuolar fusion Rab Ypt7p is conferred through downstream posttranslational modification of its effector complex...
  22. pmc Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p
    Christopher Stroupe
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755 3844, USA
    EMBO J 25:1579-89. 2006
    ..Concentration of the HOPS complex at these microdomains may be a key factor for coupling Rab GTPase activation to SNARE complex assembly...
  23. ncbi request reprint Diacylglycerol and its formation by phospholipase C regulate Rab- and SNARE-dependent yeast vacuole fusion
    Youngsoo Jun
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755 3844, USA
    J Biol Chem 279:53186-95. 2004
    ..However, vacuoles purified from plc1Delta strains still retain PLC activity and significant 3-nitrocoumarin- and U73122-sensitive fusion, suggesting that there is another PLC in S. cerevisiae with an important role in vacuole fusion...
  24. pmc Excess vacuolar SNAREs drive lysis and Rab bypass fusion
    Vincent J Starai
    Department of Biochemistry, Dartmouth Medical School, 7200 Vail Building, Hanover, NH 07355, USA
    Proc Natl Acad Sci U S A 104:13551-8. 2007
    ..Rabs function in normal membrane fusion by concentrating SNAREs, other proteins (e.g., SM), and key lipids at a fusion site and activating them for fusion without lysis...
  25. pmc Phosphoinositides function asymmetrically for membrane fusion, promoting tethering and 3Q-SNARE subcomplex assembly
    Hao Xu
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755 3844, USA
    J Biol Chem 285:39359-65. 2010
    ..These functions are essential for the phosphoinositide-dependent synergy between HOPS and Sec17p/Sec18p in promoting rapid fusion...
  26. pmc Remodeling of organelle-bound actin is required for yeast vacuole fusion
    Gary Eitzen
    Department of Biochemistry, Dartmouth Medical School, 7200 Vail Building, Hanover, NH 03755 3844, USA
    J Cell Biol 158:669-79. 2002
    ..On docked vacuoles, actin is enriched at the "vertex ring" membrane microdomain where fusion occurs and is required for the terminal steps leading to membrane fusion. This role for actin may extend to other trafficking systems...
  27. pmc Vam10p defines a Sec18p-independent step of priming that allows yeast vacuole tethering
    Masashi Kato
    Department of Biochemistry, Dartmouth Medical School, 7200 Vail Building, Hanover, NH 03755 3844, USA
    Proc Natl Acad Sci U S A 100:6398-403. 2003
    ..Pure Vam10p restores normal, Ypt7p-dependent tethering to vacuoles from a vam10Delta strain...
  28. pmc Assays of vacuole fusion resolve the stages of docking, lipid mixing, and content mixing
    Youngsoo Jun
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755 3844, USA
    Proc Natl Acad Sci U S A 104:13010-5. 2007
    ..These results support a model in which SNARE pairing leads to rapid hemifusion, followed by slow further lipid rearrangement and aqueous compartment mixing...
  29. pmc Complex lipid requirements for SNARE- and SNARE chaperone-dependent membrane fusion
    Joji Mima
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755 3844, USA
    J Biol Chem 284:27114-22. 2009
    ..Strikingly, PA and PE are as essential for SNARE complex assembly as for fusion, suggesting that these lipids facilitate functional interactions among SNAREs and SNARE chaperones...
  30. pmc Genomic analysis of homotypic vacuole fusion
    E Scott Seeley
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755 3844, USA
    Mol Biol Cell 13:782-94. 2002
    ..Our screen reveals regulatory pathways of vacuole docking and provides a genomic basis for studies of this reaction...
  31. ncbi request reprint Vacuole fusion at a ring of vertex docking sites leaves membrane fragments within the organelle
    Li Wang
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Cell 108:357-69. 2002
    ..Their vertex enrichment requires cis-SNARE complex disassembly and is thus part of the normal fusion pathway...