L R Lynd

Summary

Affiliation: Dartmouth Medical School
Country: USA

Publications

  1. ncbi request reprint Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae
    Riaan Den Haan
    Department of Microbiology, University of Stellenbosch, Stellenbosch 7600, South Africa
    Metab Eng 9:87-94. 2007
  2. doi request reprint How biotech can transform biofuels
    Lee R Lynd
    Thayer School of Engineering, Dartmouth College, 8000 Cummings Hall, Hanover, New Hampshire 03755, USA
    Nat Biotechnol 26:169-72. 2008
  3. ncbi request reprint Salt accumulation resulting from base added for pH control, and not ethanol, limits growth of Thermoanaerobacteriumthermosaccharolyticum HG-8 at elevated feed xylose concentrations in continuous culture
    L R Lynd
    Chemical and Biochemical Engineering Program, Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA
    Biotechnol Prog 17:118-25. 2001
  4. ncbi request reprint Consolidated bioprocessing of cellulosic biomass: an update
    Lee R Lynd
    Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA
    Curr Opin Biotechnol 16:577-83. 2005
  5. pmc Microbial cellulose utilization: fundamentals and biotechnology
    Lee R Lynd
    Chemical and Biochemical Engineering, Thayer School of Engineering and Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
    Microbiol Mol Biol Rev 66:506-77, table of contents. 2002
  6. ncbi request reprint Quantitative determination of cellulase concentration as distinct from cell concentration in studies of microbial cellulose utilization: analytical framework and methodological approach
    Lee R Lynd
    Chemical and Biochemical Engineering, Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA
    Biotechnol Bioeng 77:467-75. 2002
  7. pmc Cellulose- and xylan-degrading thermophilic anaerobic bacteria from biocompost
    M V Sizova
    Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
    Appl Environ Microbiol 77:2282-91. 2011
  8. pmc Cellulose utilization by Clostridium thermocellum: bioenergetics and hydrolysis product assimilation
    Yi Heng Percival Zhang
    Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
    Proc Natl Acad Sci U S A 102:7321-5. 2005
  9. ncbi request reprint Characterization of 13 newly isolated strains of anaerobic, cellulolytic, thermophilic bacteria
    M Ozkan
    Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
    J Ind Microbiol Biotechnol 27:275-80. 2001
  10. ncbi request reprint Quantification of cell and cellulase mass concentrations during anaerobic cellulose fermentation: development of an enzyme-linked immunosorbent assay-based method with application to Clostridium thermocellum batch cultures
    Yiheng Zhang
    Thayer School of Engineering and Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
    Anal Chem 75:219-27. 2003

Collaborators

Detail Information

Publications36

  1. ncbi request reprint Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae
    Riaan Den Haan
    Department of Microbiology, University of Stellenbosch, Stellenbosch 7600, South Africa
    Metab Eng 9:87-94. 2007
    ..To our knowledge, this is the first report of a recombinant strain of S. cerevisiae growing on pure cellulose...
  2. doi request reprint How biotech can transform biofuels
    Lee R Lynd
    Thayer School of Engineering, Dartmouth College, 8000 Cummings Hall, Hanover, New Hampshire 03755, USA
    Nat Biotechnol 26:169-72. 2008
    ..For cellulosic ethanol to become a reality, biotechnological solutions should focus on optimizing the conversion of biomass to sugars...
  3. ncbi request reprint Salt accumulation resulting from base added for pH control, and not ethanol, limits growth of Thermoanaerobacteriumthermosaccharolyticum HG-8 at elevated feed xylose concentrations in continuous culture
    L R Lynd
    Chemical and Biochemical Engineering Program, Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA
    Biotechnol Prog 17:118-25. 2001
    ....
  4. ncbi request reprint Consolidated bioprocessing of cellulosic biomass: an update
    Lee R Lynd
    Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA
    Curr Opin Biotechnol 16:577-83. 2005
    ..Recent studies of the fundamental principles of microbial cellulose utilization support the feasibility of CBP...
  5. pmc Microbial cellulose utilization: fundamentals and biotechnology
    Lee R Lynd
    Chemical and Biochemical Engineering, Thayer School of Engineering and Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
    Microbiol Mol Biol Rev 66:506-77, table of contents. 2002
    ....
  6. ncbi request reprint Quantitative determination of cellulase concentration as distinct from cell concentration in studies of microbial cellulose utilization: analytical framework and methodological approach
    Lee R Lynd
    Chemical and Biochemical Engineering, Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA
    Biotechnol Bioeng 77:467-75. 2002
    ..For cellulase concentration measurement, acceptable accuracy is expected when a measurement specific to cellulase such as ELISA is used. Several analytical approaches are rejected based on large expected errors...
  7. pmc Cellulose- and xylan-degrading thermophilic anaerobic bacteria from biocompost
    M V Sizova
    Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
    Appl Environ Microbiol 77:2282-91. 2011
    ..A mixture of xylan and cellulose was utilized simultaneously, with a significant synergistic effect observed as a reduction of lag phase in cellulose degradation...
  8. pmc Cellulose utilization by Clostridium thermocellum: bioenergetics and hydrolysis product assimilation
    Yi Heng Percival Zhang
    Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
    Proc Natl Acad Sci U S A 102:7321-5. 2005
    ....
  9. ncbi request reprint Characterization of 13 newly isolated strains of anaerobic, cellulolytic, thermophilic bacteria
    M Ozkan
    Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
    J Ind Microbiol Biotechnol 27:275-80. 2001
    ..The results of this study have several positive implications with respect to future development of a transformation system for cellulolytic thermophiles...
  10. ncbi request reprint Quantification of cell and cellulase mass concentrations during anaerobic cellulose fermentation: development of an enzyme-linked immunosorbent assay-based method with application to Clostridium thermocellum batch cultures
    Yiheng Zhang
    Thayer School of Engineering and Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
    Anal Chem 75:219-27. 2003
    ..thermocellum and for calculation of cell mass concentration as distinct from cellulase concentration...
  11. pmc Regulation of cellulase synthesis in batch and continuous cultures of Clostridium thermocellum
    Yi Heng Percival Zhang
    Thayer School of Engineering, 8000 Cummings Hall, Dartmouth College, Hanover, NH 03755
    J Bacteriol 187:99-106. 2005
    ..thermocellum genome...
  12. ncbi request reprint Toward an aggregated understanding of enzymatic hydrolysis of cellulose: noncomplexed cellulase systems
    Yi Heng Percival Zhang
    Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA
    Biotechnol Bioeng 88:797-824. 2004
    ....
  13. ncbi request reprint A comparison of liquid hot water and steam pretreatments of sugar cane bagasse for bioconversion to ethanol
    M Laser
    Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
    Bioresour Technol 81:33-44. 2002
    ..Achieving concurrently high (greater than 90%) SSF conversion and xylan recovery will likely require a modified reactor configuration (e.g. continuous percolation or base addition) that better preserves dissolved xylan...
  14. ncbi request reprint Cloning of L-lactate dehydrogenase and elimination of lactic acid production via gene knockout in Thermoanaerobacterium saccharolyticum JW/SL-YS485
    S G Desai
    Thayer School of Engineering, Dartmouth College, NH 03755, Hanover, USA
    Appl Microbiol Biotechnol 65:600-5. 2004
    ..The results reported here represent a step toward using metabolic engineering to develop strains of thermophilic anaerobic bacteria that do not produce organic acids, and support the methodological feasibility of this goal...
  15. pmc Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield
    A Joe Shaw
    Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
    Proc Natl Acad Sci U S A 105:13769-74. 2008
    ....
  16. doi request reprint Simultaneous saccharification and co-fermentation of paper sludge to ethanol by Saccharomyces cerevisiae RWB222--Part I: kinetic modeling and parameters
    Jiayi Zhang
    Chemical and Biochemical Engineering, Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA
    Biotechnol Bioeng 104:920-31. 2009
    ....
  17. pmc Kinetics and relative importance of phosphorolytic and hydrolytic cleavage of cellodextrins and cellobiose in cell extracts of Clostridium thermocellum
    Yi Heng Percival Zhang
    Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA
    Appl Environ Microbiol 70:1563-9. 2004
    ..The apparent K(m) for phosphorolytic cleavage was lower for cellopentaose (mean value for Avicel- and cellobiose-grown cells, 0.61 mM) than for cellobiose (mean value, 3.3 mM)...
  18. doi request reprint Simultaneous saccharification and co-fermentation of paper sludge to ethanol by Saccharomyces cerevisiae RWB222. Part II: investigation of discrepancies between predicted and observed performance at high solids concentration
    Jiayi Zhang
    Chemical and Biochemical Engineering, Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire, 03755, USA
    Biotechnol Bioeng 104:932-8. 2009
    ..A method for measuring final enzyme activity at the end of fermentation was also developed in this study...
  19. pmc Enzyme-microbe synergy during cellulose hydrolysis by Clostridium thermocellum
    Yanpin Lu
    Thayer School of Engineering and Department of Biology, Dartmouth College, Hanover, NH 03755, USA
    Proc Natl Acad Sci U S A 103:16165-9. 2006
    ..Results support the desirability of biotechnological processes featuring microbial conversion of cellulosic biomass to ethanol (or other products) in the absence of added saccharolytic enzymes...
  20. doi request reprint Ethanol production from paper sludge by simultaneous saccharification and co-fermentation using recombinant xylose-fermenting microorganisms
    Jiayi Zhang
    Chemical and Biochemical Engineering, Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
    Biotechnol Bioeng 107:235-44. 2010
    ..cerevisiae. Both organisms under optimal conditions achieved a 19% higher overall conversion of paper sludge to ethanol than the non-xylose utilizing S. cerevisiae D5A at its optimal process temperature of 37 degrees C...
  21. pmc Identification of the [FeFe]-hydrogenase responsible for hydrogen generation in Thermoanaerobacterium saccharolyticum and demonstration of increased ethanol yield via hydrogenase knockout
    A Joe Shaw
    Dartmouth College, Thayer School of Engineering, 8000 Cummings Hall, Hanover, NH 03755, USA
    J Bacteriol 191:6457-64. 2009
    ..A strain with hfs and ldh deletions exhibited an increased ethanol yield from consumed carbohydrates and represents a new strategy for engineering increased ethanol yields in T. saccharolyticum...
  22. ncbi request reprint Conversion of paper sludge to ethanol in a semicontinuous solids-fed reactor
    Zhiliang Fan
    Chemical and Biochemical Engineering Program, Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
    Bioprocess Biosyst Eng 26:93-101. 2003
    ..Run 2 produced 42 g/L ethanol at a conversion of 92%. For run 2, the ethanol yield was 0.466 g ethanol/g glucose equivalent fermented and >94% of the xylan fed to the reactor was solubilized to a mixture of xylan oligomers and xylose...
  23. ncbi request reprint Cellodextrin preparation by mixed-acid hydrolysis and chromatographic separation
    Yi Heng Percival Zhang
    Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
    Anal Biochem 322:225-32. 2003
    ..The overall procedure achieves yields comparable to the highest previously reported, employs a separation system that can readily be reused for multiple runs, and avoids use of fuming HCl...
  24. ncbi request reprint Theoretical analysis of selection-based strain improvement for microorganisms with growth dependent upon extracytoplasmic enzymes
    Zhiliang Fan
    Chemical and Biochemical Engineering Program, Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA
    Biotechnol Bioeng 92:35-44. 2005
    ..However, these factors exert a smaller impact on selection time and it is not clear that favorable values for these factors are required in order for selection to be effective...
  25. ncbi request reprint Determination of the number-average degree of polymerization of cellodextrins and cellulose with application to enzymatic hydrolysis
    Y H Percival Zhang
    Thayer School of Engineering and Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
    Biomacromolecules 6:1510-5. 2005
    ..By contrast, the DP of Avicel remained nearly constant during hydrolysis. The specific enzymatic cellulose hydrolysis rate is 100-fold higher for PASC as compared to Avicel...
  26. pmc Electrotransformation of Clostridium thermocellum
    Michael V Tyurin
    Thayer School of Engineering Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
    Appl Environ Microbiol 70:883-90. 2004
    ..thermocellum that is suitable for molecular manipulations involving either the introduction of genes associated with foreign gene products or knockout of native genes...
  27. pmc Diversity of bacteria and glycosyl hydrolase family 48 genes in cellulolytic consortia enriched from thermophilic biocompost
    Javier A Izquierdo
    Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
    Appl Environ Microbiol 76:3545-53. 2010
    ..In all, 14 new GHF48 gene sequences were added to the known diversity of 35 genes from cultured species...
  28. pmc Role of spontaneous current oscillations during high-efficiency electrotransformation of thermophilic anaerobes
    Michael V Tyurin
    Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
    Appl Environ Microbiol 71:8069-76. 2005
    ....
  29. ncbi request reprint Fractionating recalcitrant lignocellulose at modest reaction conditions
    Yi Heng Percival Zhang
    Biological Systems Engineering Department, Virginia Tech, Blacksburg, Virginia 24061, USA
    Biotechnol Bioeng 97:214-23. 2007
    ..Isolation of high-value lignocellulose components (lignin, acetic acid, and hemicellulose) would greatly increase potential revenues of a lignocellulose biorefinery...
  30. ncbi request reprint Conversion of paper sludge to ethanol. I: impact of feeding frequency and mixing energy characterization
    Zhiliang Fan
    Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Bioprocess Biosyst Eng 30:27-34. 2007
    ....
  31. ncbi request reprint A functionally based model for hydrolysis of cellulose by fungal cellulase
    Y H Percival Zhang
    Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Biotechnol Bioeng 94:888-98. 2006
    ..Opportunities to further refine cellulase kinetic models are discussed, including parameters that would benefit from further study...
  32. ncbi request reprint A transition from cellulose swelling to cellulose dissolution by o-phosphoric acid: evidence from enzymatic hydrolysis and supramolecular structure
    Y H Percival Zhang
    Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, 24060, USA
    Biomacromolecules 7:644-8. 2006
  33. ncbi request reprint Consolidated bioprocessing for bioethanol production using Saccharomyces cerevisiae
    Willem H van Zyl
    Department of Microbiology, Stellenbosch University, Private Bag X1, 7602, Matieland, South Africa
    Adv Biochem Eng Biotechnol 108:205-35. 2007
    ..Attention is also devoted to the challenges ahead to integrate all required enzymatic activities in an industrial S. cerevisiae strain(s) and the need for molecular and selection strategies pursuant to developing a yeast capable of CBP...
  34. ncbi request reprint Conversion of paper sludge to ethanol, II: process design and economic analysis
    Zhiliang Fan
    Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Bioprocess Biosyst Eng 30:35-45. 2007
    ..Our analysis lends support to the proposition that paper sludge is a leading point-of-entry and proving ground for emergent industrial processes featuring enzymatic hydrolysis of cellulosic biomass...
  35. ncbi request reprint Cloning and expression of the Clostridium thermocellum L-lactate dehydrogenase gene in Escherichia coli and enzyme characterization
    Melek Ozkan
    Biology Department, Middle East Technical University, Ankara 06531, Turkey
    Can J Microbiol 50:845-51. 2004
    ..5 mmol/L and 52 micromol/min, respectively, in its presence. The enzyme did not lose activity when incubated at 65 degrees C for 5 min...
  36. ncbi request reprint Introduction to microbial catalysis and engineering
    Thomas W Jeffries
    USDA, Forest Service, Forest Products Laboratory, Madison, WI, USA
    Appl Biochem Biotechnol 113:323-4. 2004