Research Topics
| L R LyndSummaryAffiliation: Dartmouth Medical School Country: USA Publications
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Detail Information
Publications
Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiaeRiaan Den Haan
Department of Microbiology, University of Stellenbosch, Stellenbosch 7600, South Africa
Metab Eng 9:87-94. 2007..To our knowledge, this is the first report of a recombinant strain of S. cerevisiae growing on pure cellulose...
How biotech can transform biofuelsLee R Lynd
Thayer School of Engineering, Dartmouth College, 8000 Cummings Hall, Hanover, New Hampshire 03755, USA
Nat Biotechnol 26:169-72. 2008..For cellulosic ethanol to become a reality, biotechnological solutions should focus on optimizing the conversion of biomass to sugars...- Salt accumulation resulting from base added for pH control, and not ethanol, limits growth of Thermoanaerobacteriumthermosaccharolyticum HG-8 at elevated feed xylose concentrations in continuous cultureL R Lynd
Chemical and Biochemical Engineering Program, Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA
Biotechnol Prog 17:118-25. 2001.... - Consolidated bioprocessing of cellulosic biomass: an updateLee R Lynd
Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA
Curr Opin Biotechnol 16:577-83. 2005..Recent studies of the fundamental principles of microbial cellulose utilization support the feasibility of CBP... 
Microbial cellulose utilization: fundamentals and biotechnologyLee R Lynd
Chemical and Biochemical Engineering, Thayer School of Engineering and Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
Microbiol Mol Biol Rev 66:506-77, table of contents. 2002....- Quantitative determination of cellulase concentration as distinct from cell concentration in studies of microbial cellulose utilization: analytical framework and methodological approachLee R Lynd
Chemical and Biochemical Engineering, Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA
Biotechnol Bioeng 77:467-75. 2002..For cellulase concentration measurement, acceptable accuracy is expected when a measurement specific to cellulase such as ELISA is used. Several analytical approaches are rejected based on large expected errors... - Cellulose- and xylan-degrading thermophilic anaerobic bacteria from biocompostM V Sizova
Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
Appl Environ Microbiol 77:2282-91. 2011..A mixture of xylan and cellulose was utilized simultaneously, with a significant synergistic effect observed as a reduction of lag phase in cellulose degradation... - Cellulose utilization by Clostridium thermocellum: bioenergetics and hydrolysis product assimilationYi-Heng Percival Zhang
Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
Proc Natl Acad Sci U S A 102:7321-5. 2005.... - Characterization of 13 newly isolated strains of anaerobic, cellulolytic, thermophilic bacteriaM Ozkan
Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
J Ind Microbiol Biotechnol 27:275-80. 2001..The results of this study have several positive implications with respect to future development of a transformation system for cellulolytic thermophiles... - Quantification of cell and cellulase mass concentrations during anaerobic cellulose fermentation: development of an enzyme-linked immunosorbent assay-based method with application to Clostridium thermocellum batch culturesYiheng Zhang
Thayer School of Engineering and Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
Anal Chem 75:219-27. 2003..thermocellum and for calculation of cell mass concentration as distinct from cellulase concentration... - Regulation of cellulase synthesis in batch and continuous cultures of Clostridium thermocellumYi Heng Percival Zhang
Thayer School of Engineering, 8000 Cummings Hall, Dartmouth College, Hanover, NH 03755
J Bacteriol 187:99-106. 2005..thermocellum genome... - Toward an aggregated understanding of enzymatic hydrolysis of cellulose: noncomplexed cellulase systemsYi Heng Percival Zhang
Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA
Biotechnol Bioeng 88:797-824. 2004.... - A comparison of liquid hot water and steam pretreatments of sugar cane bagasse for bioconversion to ethanolM Laser
Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
Bioresour Technol 81:33-44. 2002..Achieving concurrently high (greater than 90%) SSF conversion and xylan recovery will likely require a modified reactor configuration (e.g. continuous percolation or base addition) that better preserves dissolved xylan... - Cloning of L-lactate dehydrogenase and elimination of lactic acid production via gene knockout in Thermoanaerobacterium saccharolyticum JW/SL-YS485S G Desai
Thayer School of Engineering, Dartmouth College, NH 03755, Hanover, USA
Appl Microbiol Biotechnol 65:600-5. 2004..The results reported here represent a step toward using metabolic engineering to develop strains of thermophilic anaerobic bacteria that do not produce organic acids, and support the methodological feasibility of this goal... - Metabolic engineering of a thermophilic bacterium to produce ethanol at high yieldA Joe Shaw
Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
Proc Natl Acad Sci U S A 105:13769-74. 2008.... - Simultaneous saccharification and co-fermentation of paper sludge to ethanol by Saccharomyces cerevisiae RWB222--Part I: kinetic modeling and parametersJiayi Zhang
Chemical and Biochemical Engineering, Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA
Biotechnol Bioeng 104:920-31. 2009.... - Kinetics and relative importance of phosphorolytic and hydrolytic cleavage of cellodextrins and cellobiose in cell extracts of Clostridium thermocellumYi-Heng Percival Zhang
Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA
Appl Environ Microbiol 70:1563-9. 2004..The apparent K(m) for phosphorolytic cleavage was lower for cellopentaose (mean value for Avicel- and cellobiose-grown cells, 0.61 mM) than for cellobiose (mean value, 3.3 mM)... - Simultaneous saccharification and co-fermentation of paper sludge to ethanol by Saccharomyces cerevisiae RWB222. Part II: investigation of discrepancies between predicted and observed performance at high solids concentrationJiayi Zhang
Chemical and Biochemical Engineering, Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire, 03755, USA
Biotechnol Bioeng 104:932-8. 2009..A method for measuring final enzyme activity at the end of fermentation was also developed in this study... - Enzyme-microbe synergy during cellulose hydrolysis by Clostridium thermocellumYanpin Lu
Thayer School of Engineering and Department of Biology, Dartmouth College, Hanover, NH 03755, USA
Proc Natl Acad Sci U S A 103:16165-9. 2006..Results support the desirability of biotechnological processes featuring microbial conversion of cellulosic biomass to ethanol (or other products) in the absence of added saccharolytic enzymes... - Ethanol production from paper sludge by simultaneous saccharification and co-fermentation using recombinant xylose-fermenting microorganismsJiayi Zhang
Chemical and Biochemical Engineering, Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
Biotechnol Bioeng 107:235-44. 2010..cerevisiae. Both organisms under optimal conditions achieved a 19% higher overall conversion of paper sludge to ethanol than the non-xylose utilizing S. cerevisiae D5A at its optimal process temperature of 37 degrees C... - Identification of the [FeFe]-hydrogenase responsible for hydrogen generation in Thermoanaerobacterium saccharolyticum and demonstration of increased ethanol yield via hydrogenase knockoutA Joe Shaw
Dartmouth College, Thayer School of Engineering, 8000 Cummings Hall, Hanover, NH 03755, USA
J Bacteriol 191:6457-64. 2009..A strain with hfs and ldh deletions exhibited an increased ethanol yield from consumed carbohydrates and represents a new strategy for engineering increased ethanol yields in T. saccharolyticum... - Conversion of paper sludge to ethanol in a semicontinuous solids-fed reactorZhiliang Fan
Chemical and Biochemical Engineering Program, Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
Bioprocess Biosyst Eng 26:93-101. 2003..Run 2 produced 42 g/L ethanol at a conversion of 92%. For run 2, the ethanol yield was 0.466 g ethanol/g glucose equivalent fermented and >94% of the xylan fed to the reactor was solubilized to a mixture of xylan oligomers and xylose... - Cellodextrin preparation by mixed-acid hydrolysis and chromatographic separationYi-Heng Percival Zhang
Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
Anal Biochem 322:225-32. 2003..The overall procedure achieves yields comparable to the highest previously reported, employs a separation system that can readily be reused for multiple runs, and avoids use of fuming HCl... - Theoretical analysis of selection-based strain improvement for microorganisms with growth dependent upon extracytoplasmic enzymesZhiliang Fan
Chemical and Biochemical Engineering Program, Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA
Biotechnol Bioeng 92:35-44. 2005..However, these factors exert a smaller impact on selection time and it is not clear that favorable values for these factors are required in order for selection to be effective... - Determination of the number-average degree of polymerization of cellodextrins and cellulose with application to enzymatic hydrolysisY-H Percival Zhang
Thayer School of Engineering and Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
Biomacromolecules 6:1510-5. 2005..By contrast, the DP of Avicel remained nearly constant during hydrolysis. The specific enzymatic cellulose hydrolysis rate is 100-fold higher for PASC as compared to Avicel... - Electrotransformation of Clostridium thermocellumMichael V Tyurin
Thayer School of Engineering. Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
Appl Environ Microbiol 70:883-90. 2004..thermocellum that is suitable for molecular manipulations involving either the introduction of genes associated with foreign gene products or knockout of native genes... - Diversity of bacteria and glycosyl hydrolase family 48 genes in cellulolytic consortia enriched from thermophilic biocompostJavier A Izquierdo
Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
Appl Environ Microbiol 76:3545-53. 2010..In all, 14 new GHF48 gene sequences were added to the known diversity of 35 genes from cultured species... - Role of spontaneous current oscillations during high-efficiency electrotransformation of thermophilic anaerobesMichael V Tyurin
Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
Appl Environ Microbiol 71:8069-76. 2005.... - Fractionating recalcitrant lignocellulose at modest reaction conditionsYi Heng Percival Zhang
Biological Systems Engineering Department, Virginia Tech, Blacksburg, Virginia 24061, USA
Biotechnol Bioeng 97:214-23. 2007..Isolation of high-value lignocellulose components (lignin, acetic acid, and hemicellulose) would greatly increase potential revenues of a lignocellulose biorefinery... - Conversion of paper sludge to ethanol. I: impact of feeding frequency and mixing energy characterizationZhiliang Fan
Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
Bioprocess Biosyst Eng 30:27-34. 2007.... - A functionally based model for hydrolysis of cellulose by fungal cellulaseY H Percival Zhang
Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
Biotechnol Bioeng 94:888-98. 2006..Opportunities to further refine cellulase kinetic models are discussed, including parameters that would benefit from further study... - A transition from cellulose swelling to cellulose dissolution by o-phosphoric acid: evidence from enzymatic hydrolysis and supramolecular structureY-H Percival Zhang
Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, 24060, USA
Biomacromolecules 7:644-8. 2006 - Consolidated bioprocessing for bioethanol production using Saccharomyces cerevisiaeWillem H van Zyl
Department of Microbiology, Stellenbosch University, Private Bag X1, 7602, Matieland, South Africa
Adv Biochem Eng Biotechnol 108:205-35. 2007..Attention is also devoted to the challenges ahead to integrate all required enzymatic activities in an industrial S. cerevisiae strain(s) and the need for molecular and selection strategies pursuant to developing a yeast capable of CBP... - Conversion of paper sludge to ethanol, II: process design and economic analysisZhiliang Fan
Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
Bioprocess Biosyst Eng 30:35-45. 2007..Our analysis lends support to the proposition that paper sludge is a leading point-of-entry and proving ground for emergent industrial processes featuring enzymatic hydrolysis of cellulosic biomass... - Cloning and expression of the Clostridium thermocellum L-lactate dehydrogenase gene in Escherichia coli and enzyme characterizationMelek Ozkan
Biology Department, Middle East Technical University, Ankara 06531, Turkey
Can J Microbiol 50:845-51. 2004..5 mmol/L and 52 micromol/min, respectively, in its presence. The enzyme did not lose activity when incubated at 65 degrees C for 5 min... - Introduction to microbial catalysis and engineeringThomas W Jeffries
USDA, Forest Service, Forest Products Laboratory, Madison, WI, USA
Appl Biochem Biotechnol 113:323-4. 2004