Research Topics
Genomes and Genes
Species | Gregory J TsongalisSummaryAffiliation: Dartmouth-Hitchcock Medical Center Country: USA Publications
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Detail Information
Publications
Detection of the C282Y and H63D polymorphisms associated with hereditary hemochromatosis using the ABI 7500 fast real time PCR platformLaura J Tafe
Department of Pathology, Dartmouth Medical School, Dartmouth Hitchcock Medical Center, Lebanon, NH 03756, USA
Diagn Mol Pathol 16:112-5. 2007..Improved software and thermocycling capabilities have resulted in a very robust TAQman assay with the advantage of a much improved turn-around-time and throughput...
Polyomavirus infection of the urinary tract presenting as hemorrhagic cystitis in an immunocompetent five-year-old boyJorge L Gonzalez
Department of Pathology, Dartmouth Hitchcock Medical Center, Lebanon, New Hampshire 03756 0001, USA
Diagn Cytopathol 36:375-8. 2008..Although rare cases of nonhemorrhagic cystitis have been reported, to the best of our knowledge this is the first case of hemorrhagic cystitis occurring in this setting...- Prevalence of MTHFR gene polymorphisms (C677T and A1298C) among TamiliansT Angeline
PG and Research Department of Zoology, Lady Doak College, Madurai, Tamil Nadu, South India
Exp Mol Pathol 77:85-8. 2004..The results of this study indicate that the MTHFR A1298C SNP is more prevalent among the Tamilians when compared to the MTHFR C677T SNP, suggesting a possible role of MTHFR A1298C in the pathogenesis of heart diseases... - The effect of apolipoprotein E genotype on serum lipoprotein particle response to exerciseRichard L Seip
Cardiology Division, Hartford Hospital, 80 Seymour Street, Hartford, CT 06102 5037, USA
Atherosclerosis 188:126-33. 2006..In conclusion, APOE variants influence the serum LDL subpopulation response to exercise training in normolipidemic subjects. Subjects homozygous for APOE3 experienced the most beneficial lipid effects from exercise training... - Molecular diagnostics: parallels between infectious disease and emerging oncology testingJoel A Lefferts
Dartmouth Hitchcock Medical Center, Norris Cotton Cancer Center, Dartmouth Medical School, Department of Pathology, One Medical Center Drive, Lebanon, NH 03756, USA 1 603 650 5498 1 603 650 4845
Expert Opin Med Diagn 4:185-8. 2010.... - Pharmacogenetics: where are we with respect to personalized medicine?Joel A Lefferts
Dartmouth Medical School, Department of Pathology, Dartmouth Hitchcock Medical Center, Norris Cotton Cancer Center, One Medical Center Drive, Lebanon, NH 03756, USA 1 603 650 5498 1 603 650 4845
Expert Opin Med Diagn 1:117-28. 2007..Several national organizations are now working on practice guidelines for the incorporation of this testing into a clinical setting. This manuscript provides an overview of where we are with respect to pharmacogenetic testing... - Molecular diagnostics: a historical perspectiveGregory J Tsongalis
Department of Pathology, Dartmouth Medical School and Dartmouth Hitchcock Medical Center, One Medical Center Drive, Lebanon, NH 03756, USA
Clin Chim Acta 369:188-92. 2006..The next decade promises even more exciting technologies and applications for the field of molecular laboratory medicine... - Clinical genotyping: the need for interrogation of single nucleotide polymorphisms and mutations in the clinical laboratoryGregory J Tsongalis
Department of Pathology, Dartmouth Medical School and Dartmouth Hitchcock Medical Center Lebanon, NH 03756, USA
Clin Chim Acta 363:127-37. 2006..Detection of single nucleotide polymorphisms (SNPs) and gene mutations is becoming more routine to the clinical laboratory... 
Polymorphisms in the brain-derived neurotrophic factor gene influence memory and processing speed one month after brain injuryThomas W McAllister
Section of Neuropsychiatry, Department of Psychiatry, Dartmouth Medical School, Lebanon, New Hampshire 05756, USA
J Neurotrauma 29:1111-8. 2012..Polymorphisms in BDNF influence cognitive performance shortly after mTBI. The results raise the possibility that a functional polymorphism other than rs6265 may contribute to memory function after mTBI...- A validation study of a new molecular diagnostic assay: the Dartmouth-Hitchcock Medical Center experience with the GeneSearch BLN assay in breast sentinel lymph nodesLaura J Tafe
Translational Research Program, Department of Pathology, Dartmouth Medical School, Dartmouth Hitchcock Medical Center and Norris Cotton Cancer Center, Lebanon, NH 03756, USA
Exp Mol Pathol 88:1-6. 2010..2 mm, with our standard lymph node processing... - COMT Val158Met Genotype and Individual Differences in Executive Function in Healthy AdultsHeather A Wishart
Department of Psychiatry, Dartmouth Medical School, Lebanon, New Hampshire 03756 0001, USA
J Int Neuropsychol Soc 17:174-80. 2011..This study suggests the heterogeneity in COMT effects reported in the literature may be due in part to gene-gene interactions that influence central dopaminergic systems... - Establishment of a CYP2C19 genotyping assay for clinical useMark A Cervinski
Department of Pathology, The Geisel School of Medicine at Dartmouth, Dartmouth Hitchcock Medical Center, Lebanon, NH, USA
Am J Clin Pathol 139:202-7. 2013.... - KRAS detection in colonic tumors by DNA extraction from FTA paper: the molecular touch-prepMelissa L Petras
Department of Pathology, Dartmouth Hitchcock Medical Center, Dartmouth Medical School, Hanover, NH, USA
Diagn Mol Pathol 20:189-93. 2011..This novel means of DNA preservation from surgical specimens would benefit from additional study and validation as a dependable and practical technique to preserve specimens for molecular testing... - [MicroRNAs: novel biomarkers for human cancer]Claudine L Bartels
Department of Pathology, Darmouth Medical Schook, Darmouth Hichcock Medical Center, Lebanon, NH, USA
Ann Biol Clin (Paris) 68:263-72. 2010..The biologic roles of miRNAs in cancer suggest a correlation with prognosis and therapeutic outcome. Further investigation of these roles may lead to new approaches for the categorization, diagnosis, and treatment of human cancers... - Evaluation of the Nanosphere Verigene System and the Verigene F5/F2/MTHFR Nucleic Acid TestsJoel A Lefferts
Department of Pathology, Dartmouth Medical School, Dartmouth Hitchcock Medical Center and Norris Cotton Cancer Center, Lebanon, NH 03756, USA
Exp Mol Pathol 87:105-8. 2009..Molecular genetic testing for gene variants associated with hypercoagulability has become a standard of practice for Factor V and Factor II polymorphisms... - Molecular oncology: current trends in diagnosticsJoel A Lefferts
Dartmouth Medical School, Department of Pathology, Dartmouth Hitchcock Medical Center, One Medical Center Drive, Lebanon, NH 03756, USA
Future Oncol 4:61-70. 2008.... - Evaluating the thermostability of commercial fast real-time PCR master mixesAhmad N Abou Tayoun
Department of Pathology, Geisel School of Medicine at Dartmouth, Dartmouth Hitchcock Medical Center, Lebanon, NH 03756, USA
Exp Mol Pathol 93:261-3. 2012..Our findings have implications for clinical diagnostics in the developing world where cold-chain delivery of diagnostic assays is difficult to guarantee... - The human epidermal growth factor receptor 2 (HER2)Laura J Tafe
Department of Pathology, Dartmouth Medical School, Dartmouth Hitchcock Medical Center, Lebanon, NH, USA
Clin Chem Lab Med 50:23-30. 2012..In this manuscript, we review the biology and clinical applications of HER2 as a biomarker of disease and as a therapeutic target... - Genetic pathway-based hierarchical clustering analysis of older adults with cognitive complaints and amnestic mild cognitive impairment using clinical and neuroimaging phenotypesChantel D Sloan
Department of Genetics and Community and Family Medicine, Computational Genetics Laboratory, Dartmouth Medical School, Lebanon, New Hampshire, USA
Am J Med Genet B Neuropsychiatr Genet 153:1060-9. 2010..The results are consistent with polygenic influences on early neurodegenerative changes and demonstrate the effectiveness of hierarchical clustering in identifying genetic associations among multiple related phenotypic endpoints... - Use of a linear array for the detection of human papillomavirus genotypes in head and neck cancerCandice C Black
Department of Pathology and Medicine, Dartmouth Medical School, Dartmouth Hitchcock Medical Center, Lebanon, New Hampshire 03756, USA
Arch Pathol Lab Med 134:1813-7. 2010..The rate of oropharyngeal cancers diagnosed among Americans younger than 50 years is increasing. Infection of the oropharynx and tonsils by the human papillomavirus (HPV) has been linked to preneoplasia and cancer... - Correlation of polypoid colorectal adenocarcinoma with pre-existing adenomatous polyps and KRAS mutationHui Chen
Department of Pathology, Dartmouth Hitchcock Medical Center and Norris Cotton Cancer Center, Lebanon, NH, USA
Cancer Genet 204:245-51. 2011..In summary, grossly polypoid colorectal adenocarcinomas with a persistent pre-existing adenomatous polyp with villous architecture are strongly associated with KRAS mutations... - MicroRNAs: novel biomarkers for human cancerClaudine L Bartels
Department of Pathology, Dartmouth Medical School, Dartmouth Hitchcock Medical Center and Norris Cotton Cancer Center, Lebanon, NH 03756, USA
Clin Chem 55:623-31. 2009..These molecules have become recognized as one of the major regulatory gatekeepers of coding genes in the human genome... - Automation of genomic DNA isolation from formalin-fixed, paraffin-embedded tissuesSoya S Sam
Department of Pathology, Dartmouth Hitchcock Medical Center, Lebanon, NH 03756, United States
Pathol Res Pract 208:705-7. 2012..Comparative evaluation of three DNA isolation methods indicated that the Qiagen EZ1 method surpassed the other methods with reduced hands-on-time to produce optimum concentration of quality DNA for use in routine molecular analyses... - Evaluation of a BK virus real-time PCR assay designed using novel bioinformatics technologyJoel A Lefferts
Department of Pathology, Dartmouth Medical School and Dartmouth Hitchcock Medical Center, Lebanon, NH 03756, USA
Exp Mol Pathol 89:99-102. 2010..55 log(10) copies/mL difference between the two assays. These findings suggest that despite small differences, the IntelligentMDx assay could be adopted for clinical BKV monitoring of renal transplant patients... - Single nucleotide polymorphisms in ANKK1 and the dopamine D2 receptor gene affect cognitive outcome shortly after traumatic brain injury: a replication and extension studyThomas W McAllister
Department of Psychiatry, Section of Neuropsychiatry, Dartmouth Hitchcock Medical Center, Lebanon, NH 03756, USA
Brain Inj 22:705-14. 2008.... - The practical application of chimerism analyses in allogeneic stem cell transplant recipients: blood chimerism is equivalent to marrow chimerismCocav A Rauwerdink
Blood and Marrow Transplant Program, Section of Hematology and Oncology, Dartmouth Hitchcock Medical Center, Geisel School of Medicine at Dartmouth, and the Norris Cotton Cancer Center, Lebanon, NH 03756, USA
Exp Mol Pathol 93:339-44. 2012..We performed a retrospective analysis of 42 consecutive adult allogeneic SCT recipients at our institution to determine if both sample types are needed... - Cytomorphologic features of advanced lung adenocarcinomas tested for EGFR and KRAS mutations: a retrospective review of 50 casesJonathan D Marotti
Department of Pathology, Dartmouth Hitchcock Medical Center, Lebanon, New Hampshire 03756, USA
Diagn Cytopathol 41:15-21. 2013..039) correlated with the presence of EGFR mutations. The presence of necrosis (P = 0.030), squamoid features (P = 0.048), and poorly differentiated tumors (P = 0.025) were more likely to be identified in the KRAS positive group... - Validation of a CYP2D6 genotyping panel on the NanoChip Molecular Biology WorkstationHong Kee Lee
Department of Pathology, Dartmouth Medical School, Dartmouth Hitchcock Medical Center, Lebanon, NH, USA
Clin Chem 53:823-8. 2007..CYP2D6 is a highly polymorphic phase I enzyme that metabolizes 20%-25% of clinically used drugs. The objective of this study was to validate a CYP2D6 genotyping assay with the NanoChip Molecular Biology Workstation... - Quantitative assessment of the BCR-ABL transcript using the Cepheid Xpert BCR-ABL Monitor assayScott D Dufresne
Department of Pathology, Dartmouth Medical School, Dartmouth Hitchcock Medical Center, One Medical Center Dr, Lebanon, NH 03759, USA
Arch Pathol Lab Med 131:947-50. 2007.... - MicroRNAs in chronic lymphocytic leukemiaBrian P Ward
Department of Pathology, Dartmouth Hitchcock Medical Center, Lebanon, NH, USA
Exp Mol Pathol 90:173-8. 2011..Because miRNA expression levels in CLL patients differ from that of normal patients, there may be a role for these novel small molecules as biomarkers in this disease... - Negative urine opioid screening caused by rifampin-mediated induction of oxycodone hepatic metabolismHong-Kee Lee
Department of Pathology, Dartmouth Medical School and Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756, United States
Clin Chim Acta 367:196-200. 2006..CONCLUSION: This case demonstrates how the combination of analytical toxicology and pharmacogenetic analyses enhances a physician's ability to personalize drug therapy in patients with chronic pain syndromes... - BRCA1 and BRCA2 mutation screening using SmartCycler II high-resolution melt curve analysisScott D Dufresne
Department of Pathology, Dartmouth Medical School, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756-0001, USA
Arch Pathol Lab Med 130:185-7. 2006..As a proof of principle, we used this assay to identify the 3 most common BRCA1 and BRCA2 mutations in the Ashkenazi Jewish population... - Real-time polymerase chain reaction and laser capture microdissection for the diagnosis of BK virus infection in renal allograftsOyedele A Adeyi
Department of Pathology, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756, USA
Am J Clin Pathol 124:537-42. 2005..This technique detected BKV in tubular epithelial cells of renal allografts. To our knowledge, this is the first report of detecting BKV in laser capture microdissected renal biopsy specimens using real-time PCR... - Genetic testing: current and future trendsGregory J Tsongalis
Department of Pathology, Dartmouth-Hitchcock Medical Center, Dartmouth Medical School, New Hampshire, USA
MLO Med Lab Obs 38:42, 44. 2006 - Prevalence of the Factor V G1691A and the Factor II/prothrombin G20210A gene polymorphisms among TamiliansT Angeline
PG and Research Department of Zoology, Lady Doak College, Madurai, Tamil Nadu, India
Exp Mol Pathol 79:9-13. 2005..In addition, the results of this study do not support a role for these SNPs in acute myocardial infarction in the Tamilian population... - Apolipoprotein A1 genotype affects the change in high density lipoprotein cholesterol subfractions with exercise trainingGUALBERTO RUANO
Genomas, Inc, 67 Jefferson Street, Hartford, CT 06106, USA
Atherosclerosis 185:65-9. 2006..3+/-6.6 mg/dL versus 4.7+/-1.2 mg/dL, p<0.005). These results show that genetic variation at the APOA1 gene promoter is associated with HDL subfraction redistribution resulting from exercise training... - The alpha-adducin Gly460Trp polymorphism and the antihypertensive effects of exercise among men with high blood pressureLinda S Pescatello
Department of Kinesiology, University of Connecticut, Storrs, CT, USA
Clin Sci (Lond) 113:251-8. 2007..Our findings indicate that the alpha-adducin Gly460Trp genotype may be useful in identifying men who have a reduced BP after lower intensity aerobic exercise... - A reality check for molecular diagnostics in clinical practiceGregory J Tsongalis
Department of Pathology and Laboratory Medicine, Hartford Hospital, 80 Seymour Street, Hartford, CT 06102, USA
Pharmacogenomics 4:667-8. 2003 - DNA STAT: the ability to perform clinical analysis in real timeGregory J Tsongalis
Expert Rev Mol Diagn 5:627-8. 2005 - Angiotensin-converting enzyme genotype and adherence to aerobic exercise trainingPaul D Thompson
Hartford Hospital, Hartford, CT 06102, USA
Prev Cardiol 9:21-4. 2006..These preliminary results suggest that ACE genotype affects exercise adherence and raises the possibility that superior endurance capacity in subjects with the I allele is related to a genetic propensity to adhere to an exercise regimen... - MTHFR Gene polymorphisms, B-vitamins and hyperhomocystinemia in young and middle-aged acute myocardial infarction patientsT Angeline
PG and Research Department of Zoology, Lady Doak College, Madurai 625 002, Tamil Nadu, South India
Exp Mol Pathol 82:227-33. 2007.... - RAAS polymorphisms alter the acute blood pressure response to aerobic exercise among men with hypertensionBruce E Blanchard
University of CT, Storrs, CT 06102, USA
Eur J Appl Physiol 97:26-33. 2006..In order to optimally prescribe exercise for its BP lowering benefits in those with hypertension, additional knowledge of how genetic variation affects the BP response to exercise is needed...