Research Topics
| PATRICK SWANSONSummaryAffiliation: Creighton University Country: USA Publications
Research Grants
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Publications
HMG-box domain stimulation of RAG1/2 cleavage activity is metal ion dependentAleksei N Kriatchko
Department of Medical Microbiology and Immunology, Creighton University Medical Center, 2500 California Plaza, Omaha, NE, USA
BMC Mol Biol 9:32. 2008..Here we test whether this apparent discrepancy is attributed to the choice of divalent metal ion and the concentration of HMGB1 used in the cleavage reaction...- HMGB1/2 can target DNA for illegitimate cleavage by the RAG1/2 complexMing Zhang
Department of Medical Microbiology and Immunology, Creighton University Medical Center, Omaha, NE, USA
BMC Mol Biol 10:24. 2009.... - The DDE motif in RAG-1 is contributed in trans to a single active site that catalyzes the nicking and transesterification steps of V(D)J recombinationP C Swanson
Department of Medical Microbiology and Immunology, Creighton University, School of Medicine, Omaha, Nebraska 68178, USA
Mol Cell Biol 21:449-58. 2001..The implications of a trans cleavage mode observed in these complexes on the organization of the V(D)J synaptic complex are discussed... 
Early steps of V(D)J rearrangement: insights from biochemical studies of RAG-RSS complexesPatrick C Swanson
Department of Medical Microbiology and Immunology, Creighton University Medical Center, Omaha, Nebraska 68178, USA
Adv Exp Med Biol 650:1-15. 2009..In this chapter we will review the molecular mechanisms of RAG synaptic complex assembly and 12/23-regulated RSS cleavage, focusing on recent advances that shed new light on these processes...- The bounty of RAGs: recombination signal complexes and reaction outcomesPatrick C Swanson
Department of Medical Microbiology and Immunology, Creighton University Medical Center, Omaha, NE 68178, USA
Immunol Rev 200:90-114. 2004..Finally, I discuss alternative DNA transactions mediated by the V(D)J recombinase, the protein-DNA complexes that support them, and factors and forces that control them... - A RAG-1/RAG-2 tetramer supports 12/23-regulated synapsis, cleavage, and transposition of V(D)J recombination signalsPatrick C Swanson
Department of Medical Microbiology and Immunology, School of Medicine, Creighton University, Omaha, Nebraska 68178, USA
Mol Cell Biol 22:7790-801. 2002..Interestingly, the RAG-1/RAG-2 tetramer also supports transposition, but HMG-1 is dispensable for its activity... - Fine structure and activity of discrete RAG-HMG complexes on V(D)J recombination signalsPatrick C Swanson
Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska 68178, USA
Mol Cell Biol 22:1340-51. 2002..Thus, HMG protein plays the dual role of bringing critical elements of the 23-RSS heptamer into the same phase as the 12-RSS to promote RAG binding and assisting in the catalysis of 23-RSS cleavage... - Full-length RAG-2, and not full-length RAG-1, specifically suppresses RAG-mediated transposition but not hybrid joint formation or disintegrationPatrick C Swanson
Department of Medical Microbiology and Immunology, Creighton University Medical Center, Omaha, Nebraska 68178, USA
J Biol Chem 279:4034-44. 2004.... - Identification and characterization of a gain-of-function RAG-1 mutantAleksei N Kriatchko
Dept of Medical Microbiology and Immunology, Creighton University Medical Center, 2500 California Plaza, Omaha, NE 68178, USA
Mol Cell Biol 26:4712-28. 2006..These data suggest that the E649A RAG-1 mutation may interfere with the RAG proteins' ability to sense 12/23-regulated synapsis... - Both high mobility group (HMG)-boxes and the acidic tail of HMGB1 regulate recombination-activating gene (RAG)-mediated recombination signal synapsis and cleavage in vitroSerge Bergeron
Department of Medical Microbiology and Immunology, Creighton University Medical Center, Omaha, Nebraska 68178, USA
J Biol Chem 280:31314-24. 2005..Box A or B mutants fail to promote RAG synaptic complex formation, but this defect is alleviated when the acidic tail is removed from these mutants... - Molecular mechanism underlying RAG1/RAG2 synaptic complex formationLuda S Shlyakhtenko
Department of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
J Biol Chem 284:20956-65. 2009..The replacement of Mg2+ cations with Ca2+ leads to a dramatic change in protein stoichiometry for all RAG-RSS complexes, suggesting that the cation composition profoundly influences the type of complex assembled... - RAG and HMGB1 proteins: purification and biochemical analysis of recombination signal complexesSerge Bergeron
Creighton University, Medical Microbiology and Immunology, Omaha, Nebraska, USA
Methods Enzymol 408:511-28. 2006..In addition, it details several experimental procedures used in our laboratory to study RAG-RSS complex formation and function in vitro... - Full-length RAG1 promotes contact with coding and intersignal sequences in RAG protein complexes bound to recombination signals paired in cisSushil Kumar
Department of Medical Microbiology and Immunology, Creighton University Medical Center, Omaha, Nebraska 68178, USA
Nucleic Acids Res 37:2211-26. 2009..These results provide new insights into the mechanism of physiological V(D)J recombination... 
V(D)J recombinase binding and cleavage of cryptic recombination signal sequences identified from lymphoid malignanciesMing Zhang
Department of Medical Microbiology and Immunology, Creighton University Medical Center, Omaha, Nebraska 68178, USA
J Biol Chem 283:6717-27. 2008..In contrast, Hox11 and SCL are nicked but not cleaved efficiently in vitro, and cleavage at other adventitious sites in plasmid substrates may also limit the ability to detect recombination activity at these cRSSs in cell culture...- Evidence for Ku70/Ku80 association with full-length RAG1Prafulla Raval
Department of Medical Microbiology and Immunology, Creighton University Medical Center, Omaha, NE 68178, USA
Nucleic Acids Res 36:2060-72. 2008..Here we present evidence that two components of the NHEJ pathway, Ku70 and Ku80, interact with full-length RAG1, providing a biochemical link between the two phases of V(D)J recombination... - A biochemically defined system for coding joint formation in V(D)J recombinationHaihui Lu
Norris Comprehensive Cancer Center, Room 5428, University of Southern California Keck School of Medicine, 1441 Eastlake Avenue, MC9176, Los Angeles, CA 90089, USA
Mol Cell 31:485-97. 2008.... - A non-B-DNA structure at the Bcl-2 major breakpoint region is cleaved by the RAG complexSathees C Raghavan
Norris Comprehensive Cancer Center, Room 5428, University of Southern California Keck School of Medicine, 1441 Eastlake Ave, MC9176, Los Angeles, California 90033, USA
Nature 428:88-93. 2004..Hence, a stable non-B-DNA structure in the human genome appears to be the basis for the fragility of the Bcl-2 Mbr, and the RAG complex is able to cleave this structure... - Fluorescence resonance energy transfer analysis of recombination signal sequence configuration in the RAG1/2 synaptic complexMihai Ciubotaru
Howard Hughes Medical Institute and Department of Immunibiology, Yale University School of Medicine, New Haven, CT 06520 8011, USA
Mol Cell Biol 27:4745-58. 2007.... - Double-strand break formation by the RAG complex at the bcl-2 major breakpoint region and at other non-B DNA structures in vitroSathees C Raghavan
Norris Comprehensive Cancer Center, Rm. 5428, University of Southern California Keck School of Medicine, 1441 Eastlake Ave, MC9176, Los Angeles, California 90033, USA
Mol Cell Biol 25:5904-19. 2005..Hence, the present study reveals novel insight into a third mechanism of action of RAGs on DNA, besides the standard heptamer/nonamer-mediated cleavage in V(D)J recombination and the in vitro transposase activity... - The structure-specific nicking of small heteroduplexes by the RAG complex: implications for lymphoid chromosomal translocationsSathees C Raghavan
Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India
DNA Repair (Amst) 6:751-9. 2007..We consider these findings in the context of RAG nicking at non-B DNA structures in lymphoid chromosomal translocations...
Research Grants
- Characterization of V(D)J cleavage and repair complexesPATRICK SWANSON; Fiscal Year: 2007..abstract_text> ..