Ye Fang

Summary

Affiliation: Corning Incorporated
Country: USA

Publications

  1. doi request reprint Label-free optical biosensor for ligand-directed functional selectivity acting on beta(2) adrenoceptor in living cells
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Sullivan Park, Corning, NY 14831, USA
    FEBS Lett 582:558-64. 2008
  2. ncbi request reprint Optical biosensor provides insights for bradykinin B(2) receptor signaling in A431 cells
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Sullivan Park, Corning, NY 14831, USA
    FEBS Lett 579:6365-74. 2005
  3. ncbi request reprint Non-invasive optical biosensor for assaying endogenous G protein-coupled receptors in adherent cells
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Sullivan Park, Corning, NY 14831, USA
    J Pharmacol Toxicol Methods 55:314-22. 2007
  4. ncbi request reprint Label-free cell-based assays with optical biosensors in drug discovery
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Inc, Sullivan Park, Corning, NY 14831, USA
    Assay Drug Dev Technol 4:583-95. 2006
  5. ncbi request reprint The development of label-free cellular assays for drug discovery
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning, Inc, Corning, NY 14831, USA
    Expert Opin Drug Discov 6:1285-98. 2011
  6. pmc GPCRs regulate the assembly of a multienzyme complex for purine biosynthesis
    Florence Verrier
    Biochemical Technologies, Science and Technology Division, Corning Inc, Corning, New York, USA
    Nat Chem Biol 7:909-15. 2011
  7. doi request reprint Divergent label-free cell phenotypic pharmacology of ligands at the overexpressed β₂-adrenergic receptors
    Ann M Ferrie
    1 Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY 14831, United States of America 2
    Sci Rep 4:3828. 2014
  8. pmc Label-free integrative pharmacology on-target of drugs at the β(2)-adrenergic receptor
    Ann M Ferrie
    Biochemical Technologies, Science and Technology Division, Corning Inc, Corning, NY 14831, USA
    Sci Rep 1:33. 2011
  9. pmc Multiple tyrosine metabolites are GPR35 agonists
    Huayun Deng
    Biochemical Technologies, Science and Technology Division, Corning Inc, Corning, NY 14831, United States
    Sci Rep 2:373. 2012
  10. pmc Agonist-directed desensitization of the β2-adrenergic receptor
    Vasiliy Goral
    Biochemical Technologies, Science and Technology Division, Corning Inc, Corning, New York, United States of America
    PLoS ONE 6:e19282. 2011

Collaborators

Detail Information

Publications52

  1. doi request reprint Label-free optical biosensor for ligand-directed functional selectivity acting on beta(2) adrenoceptor in living cells
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Sullivan Park, Corning, NY 14831, USA
    FEBS Lett 582:558-64. 2008
    ..This study documents multiple ligand-specific states of the beta(2)-adrenoceptor and highlights the power of the biosensor assays for screening pathway-biased ligands...
  2. ncbi request reprint Optical biosensor provides insights for bradykinin B(2) receptor signaling in A431 cells
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Sullivan Park, Corning, NY 14831, USA
    FEBS Lett 579:6365-74. 2005
    ..The roles of endocytosis and cytoskeleton modulation in B(2) signaling were also demonstrated...
  3. ncbi request reprint Non-invasive optical biosensor for assaying endogenous G protein-coupled receptors in adherent cells
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Sullivan Park, Corning, NY 14831, USA
    J Pharmacol Toxicol Methods 55:314-22. 2007
    ..The present study is focused on the use of non-invasive and manipulation-free optical biosensors for assaying endogenous GPCRs in adherent cells...
  4. ncbi request reprint Label-free cell-based assays with optical biosensors in drug discovery
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Inc, Sullivan Park, Corning, NY 14831, USA
    Assay Drug Dev Technol 4:583-95. 2006
    ..This article reviews important advances in optical biosensor instrumentation, and highlights the potential of optical biosensors for drug discovery with an emphasis on whole cell sensing in both high throughput and high content fashions...
  5. ncbi request reprint The development of label-free cellular assays for drug discovery
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning, Inc, Corning, NY 14831, USA
    Expert Opin Drug Discov 6:1285-98. 2011
    ..These challenges will need to be met for the wide adoption of these assays in drug discovery...
  6. pmc GPCRs regulate the assembly of a multienzyme complex for purine biosynthesis
    Florence Verrier
    Biochemical Technologies, Science and Technology Division, Corning Inc, Corning, New York, USA
    Nat Chem Biol 7:909-15. 2011
    ....
  7. doi request reprint Divergent label-free cell phenotypic pharmacology of ligands at the overexpressed β₂-adrenergic receptors
    Ann M Ferrie
    1 Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY 14831, United States of America 2
    Sci Rep 4:3828. 2014
    ..We demonstrate that receptor signaling and ligand pharmacology is sensitive to the receptor expression level, and the organization of the receptor and its signaling circuitry. ..
  8. pmc Label-free integrative pharmacology on-target of drugs at the β(2)-adrenergic receptor
    Ann M Ferrie
    Biochemical Technologies, Science and Technology Division, Corning Inc, Corning, NY 14831, USA
    Sci Rep 1:33. 2011
    ..We demonstrate that the approach establishes an effective link between the label-free pharmacology and in vivo therapeutic indications of drugs...
  9. pmc Multiple tyrosine metabolites are GPR35 agonists
    Huayun Deng
    Biochemical Technologies, Science and Technology Division, Corning Inc, Corning, NY 14831, United States
    Sci Rep 2:373. 2012
    ..These results suggest that multiple tyrosine metabolites are alternative endogenous ligands of GPR35, and GPR35 may represent a druggable target for treating certain diseases associated with abnormality of tyrosine metabolism...
  10. pmc Agonist-directed desensitization of the β2-adrenergic receptor
    Vasiliy Goral
    Biochemical Technologies, Science and Technology Division, Corning Inc, Corning, New York, United States of America
    PLoS ONE 6:e19282. 2011
    ..This study reveals the cellular mechanisms of agonist-selective β(2)AR desensitization at the whole cell level...
  11. pmc Label-free phenotypic profiling identified D-luciferin as a GPR35 agonist
    Haibei Hu
    Biochemical Technologies, Science and Technology Division, Corning Inc, Corning, New York, United States of America
    PLoS ONE 7:e34934. 2012
    ..These results not only suggest that D-luciferin is a partial agonist of GPR35, but also will evoke careful interpretation of biological data obtained using molecular and in vivo imaging assays when these probe molecules are used...
  12. pmc Discovery of 2-(4-methylfuran-2(5H)-ylidene)malononitrile and thieno[3,2-b]thiophene-2-carboxylic acid derivatives as G protein-coupled receptor 35 (GPR35) agonists
    Huayun Deng
    Biochemical Technologies, Science and Technology Division, Corning Inc, Corning, New York 14831, United States
    J Med Chem 54:7385-96. 2011
    ..The present study provides novel chemical series as a starting point for further investigations of GPR35 biology and pharmacology...
  13. ncbi request reprint Label-free drug discovery
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated Corning, NY, USA
    Front Pharmacol 5:52. 2014
    ....
  14. doi request reprint A label-free optical biosensor with microfluidics identifies an intracellular signalling wave mediated through the β(2)-adrenergic receptor
    Ann M Ferrie
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY 14831, USA
    Integr Biol (Camb) 5:1253-61. 2013
    ....
  15. pmc Label-free integrative pharmacology on-target of opioid ligands at the opioid receptor family
    Megan Morse
    Department of Pharmacology, Pennsylvania State University College of Medicine, Hershey, PA, USA
    BMC Pharmacol Toxicol 14:17. 2013
    ..DMR profiles were collected and translated to numerical coordinates that was subject to similarity analysis. A specific set of opioid ligands were then selected for quantitative pharmacology determination...
  16. ncbi request reprint G protein-coupled receptor microarrays for drug discovery
    Ye Fang
    Biochemical Sciences Science and Technology, Division Corning Incorporated, Sullivan Park, Corning, NY 14831, USA
    Drug Discov Today 9:S61-7. 2004
    ..Assays to screen compounds for their pharmacological properties (binding affinity, relative potency and selectivity) using GPCR microarrays are discussed...
  17. doi request reprint Troubleshooting and deconvoluting label-free cell phenotypic assays in drug discovery
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY 14831, USA
    J Pharmacol Toxicol Methods 67:69-81. 2013
    ....
  18. ncbi request reprint Ligand-receptor interaction platforms and their applications for drug discovery
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning, Inc, Corning, NY 14831, USA
    Expert Opin Drug Discov 7:969-88. 2012
    ..Nowadays, ligand-binding assays can determine every aspect of many drug-target interactions...
  19. ncbi request reprint Live cell optical sensing for high throughput applications
    Ye Fang
    Biochemical Department, Science and Technology Division, Corning Incorporated, Corning, New York, 14831, USA
    Adv Biochem Eng Biotechnol 118:153-63. 2010
    ..This article describes the uses of live cell optical sensing for probing cell biology and ligand pharmacology, with an emphasis of resonant waveguide grating biosensor cellular assays for high throughput applications...
  20. ncbi request reprint Label-free cell-based assays for GPCR screening
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY 14831, USA
    Comb Chem High Throughput Screen 11:357-69. 2008
    ..This article reviews the principles and potential of current label-free cell assay technologies in GPCR drug discovery...
  21. pmc Optical biosensor differentiates signaling of endogenous PAR1 and PAR2 in A431 cells
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Sullivan Park, Corning, NY 14831, USA
    BMC Cell Biol 8:24. 2007
    ..Protease activated receptors (PARs) consist of a family of four G protein-coupled receptors. Many types of cells express several PARs, whose physiological significance is mostly unknown...
  22. ncbi request reprint Optical biosensors for monitoring dynamic mass redistribution in living cells mediated by epidermal growth factor receptor activation
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Sullivan Park, Corning, NY 14831, USA phone 607 974 7203 fax 607 974 5957 e mail
    Conf Proc IEEE Eng Med Biol Soc 1:666-9. 2005
    ....
  23. ncbi request reprint G protein-coupled receptor microarrays for drug discovery
    Ye Fang
    Biochemical Sciences, Science and Technology Division, Corning Incorporated, Sullivan Park, Corning NY 14831, USA
    Drug Discov Today 8:755-61. 2003
    ..Assays to screen compounds for their pharmacological properties (binding affinity, relative potency and selectivity) using GPCR microarrays are discussed...
  24. ncbi request reprint Probing cytoskeleton modulation by optical biosensors
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Sullivan Park, Corning, NY 14870, USA
    FEBS Lett 579:4175-80. 2005
    ..These results suggested that optical biosensors provide an attractive means to study the cytoskeleton structure and screen modulators that affect the cytoskeleton structure...
  25. ncbi request reprint Air-stable G protein-coupled receptor microarrays and ligand binding characteristics
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Sullivan Park, Corning, New York 14831, USA
    Anal Chem 78:149-55. 2006
    ..The potential mechanisms and implications for the uses of air-stable GPCR microarrays were discussed...
  26. ncbi request reprint Cellular functions of cholesterol probed with optical biosensors
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Sullivan Park, Corning, NY 14831, USA
    Biochim Biophys Acta 1763:254-61. 2006
    ....
  27. pmc Resonant waveguide grating biosensor for living cell sensing
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, New York 14831, USA
    Biophys J 91:1925-40. 2006
    ..This study provides fundamentals for probing living cells with the RWG biosensors, in general, optical biosensors...
  28. ncbi request reprint Characteristics of dynamic mass redistribution of epidermal growth factor receptor signaling in living cells measured with label-free optical biosensors
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Sullivan Park, Corning, New York 14831, USA
    Anal Chem 77:5720-5. 2005
    ..The DMR signals obtained serve as integrated signatures for interaction networks in the EGFR signaling...
  29. ncbi request reprint Membrane biochips
    Ye Fang
    Science and Technology Division, Corning Incorporated, Corning, NY, USA
    Biotechniques . 2002
    ..Ligand binding selectivity and specificity to G protein-coupled receptor (GPCR) microarrays are presented. The potential applications of these arrays for drug screening are discussed...
  30. ncbi request reprint Fabrication and application of G protein-coupled receptor microarrays
    Ye Fang
    Science and Technology Division, Biochemical Technologies, Corning, Inc, Corning, NY, USA
    Methods Mol Biol 264:233-43. 2004
    ..The bioassays and potential applications of GPCR microarrays for the determination of compound affinities and potencies are also included...
  31. doi request reprint Thieno[3,2-b]thiophene-2-carboxylic acid derivatives as GPR35 agonists
    Huayun Deng
    Biochemical Technologies, Science and Technology Division, Corning Inc, Corning, NY 14831, USA
    Bioorg Med Chem Lett 22:4148-52. 2012
    ..Compound 13 was identified to the most potent GPR35 agonist, and compounds 30 and 36 exhibited the highest efficacy to cause β-arrestin translocation...
  32. ncbi request reprint G-protein-coupled receptor microarrays for multiplexed compound screening
    Yulong Hong
    Corning Incorporated, Corning, NY, USA
    J Biomol Screen 11:435-8. 2006
    ..Taken together, these features make the system ideally suited to explore the structure-activity relationship of compounds across a broad region of target class space...
  33. doi request reprint Label-free optical biosensor with microfluidics for sensing ligand-directed functional selectivity on trafficking of thrombin receptor
    Vasiliy Goral
    Biochemical Technologies, Science and Technology Division, Corning Inc, Corning, NY 14831, United States
    FEBS Lett 585:1054-60. 2011
    ..This study demonstrates a new means to differentiate ligand-directed functional selectivity on trafficking of receptors...
  34. doi request reprint Tyrphostin analogs are GPR35 agonists
    Huayun Deng
    Biochemical Technologies, Science and Technology Division, Corning Inc, Corning, NY 14831, United States
    FEBS Lett 585:1957-62. 2011
    ..These agonists provide pharmacological tools to study the biology and function of GPR35...
  35. doi request reprint Resonant waveguide grating biosensor for whole-cell GPCR assays
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY, USA
    Methods Mol Biol 552:239-52. 2009
    ..These biosensor cellular assays hold the potential to reduce attrition rates in drug discovery and development process...
  36. ncbi request reprint Functional GPCR microarrays
    Yulong Hong
    Science and Technology Division, Corning Inc, Corning, NY 14831, USA
    J Am Chem Soc 127:15350-1. 2005
    ..Such microarrays could potentially streamline drug discovery by helping integrate primary screening with selectivity and safety screening without compromising the essential functional information obtainable from cellular assays...
  37. doi request reprint Probing biochemical mechanisms of action of muscarinic M3 receptor antagonists with label-free whole cell assays
    Huayun Deng
    Biochemical Technologies, Science and Technology Division, Corning Inc, Corning, New York 14831, USA
    Anal Chem 84:8232-9. 2012
    ..This study demonstrates that DMR assays can be used to elucidate the functional consequence of kinetics-driven antagonist occupancy in native cells...
  38. ncbi request reprint G-protein-coupled receptor microarrays
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY 14831, USA
    Chembiochem 3:987-91. 2002
    ....
  39. ncbi request reprint Label-free optical biosensor for probing integrative role of adenylyl cyclase in G protein-coupled receptor signaling
    Elizabeth Tran
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Sullivan Park, Corning, New York, USA
    J Recept Signal Transduct Res 29:154-62. 2009
    ..This study documents the integrative role of adenylyl cyclase in GPCR signaling and the power of forskolin as a pharmacological probe to differentiate receptor signaling using the label-free biosensor cellular assays...
  40. ncbi request reprint Dynamic mass redistribution assay decodes differentiation of a neural progenitor stem cell
    Sadashiva Pai
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY 14831, USA
    J Biomol Screen 17:1180-91. 2012
    ..These findings suggest that DMR assays can decode the differentiation process of stem cells at the cell system level...
  41. pmc Dynamic mass redistribution assays decode surface influence on signaling of endogenous purinergic P2Y receptors
    Elizabeth Tran
    Science and Technology Division, Department of Biochemical Technologies, Corning Incorporated, Corning, New York 14831, USA
    Assay Drug Dev Technol 10:37-45. 2012
    ..Results showed that ECM proteins had significant impacts on the DMR characteristics, potency, and efficacy of seven P2Y agonists. This study documents the importance of surface chemistry in regulating receptor signaling...
  42. doi request reprint Modulating cell-cell communication with a high-throughput label-free cell assay
    Guangshan Li
    Corning Inc, Biochemical Technologies, Corning, NY 14831, USA
    J Lab Autom 17:6-15. 2012
    ..Distinct optical signals could be generated with the pretreatment of the target cells with various known pharmaceutical reagents, making the assay useful for discovering new chemicals that may affect cell-cell communications...
  43. doi request reprint GPCR microspot assays on solid substrates
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY, USA
    Methods Mol Biol 552:231-8. 2009
    ..The chapter describes air-stable GPCR microarrays and their utility for selectivity profiling of GPCR drugs with high fidelity...
  44. ncbi request reprint Label-free cell phenotypic profiling decodes the composition and signaling of an endogenous ATP-sensitive potassium channel
    Haiyan Sun
    1 Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY 14831, United States of America 2
    Sci Rep 4:4934. 2014
    ..The results are the first demonstration of a label-free methodology to characterize the composition and signaling of an endogenous ATP-sensitive potassium ion channel. ..
  45. ncbi request reprint Spreading and segregation of lipids in air-stable lipid microarrays
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Inc, Sullivan Park, New York 14831, USA
    J Am Chem Soc 128:3158-9. 2006
    ....
  46. doi request reprint Anti-inflammatory gallic Acid and wedelolactone are G protein-coupled receptor-35 agonists
    Huayun Deng
    Biochemical Technologies, Science and Technology Division, Corning Inc, Corning, N Y 14830, USA
    Pharmacology 89:211-9. 2012
    ..GPR35 may represent a target for the treatment of allergic disorders including asthma...
  47. doi request reprint Aspirin metabolites are GPR35 agonists
    Huayun Deng
    Biochemical Technologies, Science and Technology Division, Corning Inc, Corning, NY 14831, USA
    Naunyn Schmiedebergs Arch Pharmacol 385:729-37. 2012
    ..Salicyluric acid, the main metabolite of aspirin, was also active. These results suggest that the GPR35 agonist activity of certain aspirin metabolites may contribute to the clinical features of aspirin...
  48. ncbi request reprint Membrane protein microarrays
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY 14831, USA
    J Am Chem Soc 124:2394-5. 2002
    ..This first ever demonstration of direct pin-printing of membrane proteins and ligand-binding assays thereof fills a significant void in protein microchip technology--the lack of practical microarray-based methods for membrane proteins...
  49. pmc Label-free cell phenotypic assessment of the biased agonism and efficacy of agonists at the endogenous muscarinic M3 receptors
    Huayun Deng
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY 14831, United States
    J Pharmacol Toxicol Methods 68:323-33. 2013
    ..Here we characterize the biased agonism and cell phenotypic efficacy of seven agonists at the endogenous muscarinic M3 receptors in six different cell lines including HT-29, PC-3, HeLa, SF268, CCRF-CEM and HCT-15 cells...
  50. ncbi request reprint G-protein-coupled receptor regulation of de novo purine biosynthesis: a novel druggable mechanism
    Ye Fang
    a Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, New York, USA
    Biotechnol Genet Eng Rev 29:31-48. 2013
    ..This paper also discusses the implications of purinosome and GPCR regulation in drug discovery...
  51. doi request reprint Evaluation of dynamic mass redistribution technology for pharmacological studies of recombinant and endogenously expressed g protein-coupled receptors
    Paul H Lee
    Chemistry Research and Discovery, Amgen Inc, Thousand Oaks, California, USA
    Assay Drug Dev Technol 6:83-94. 2008
    ..The Epic DMR technology provides a generic platform amenable to pharmacological evaluation of cellular responses to GPCR activation in a label-free live cell assay format...
  52. ncbi request reprint Multiplexing G protein-coupled receptors in microarrays: a radioligand-binding assay
    Bruce Posner
    Pfizer, Groton, CT 06340, USA
    Anal Biochem 365:266-73. 2007
    ..The impact of this approach on screening in drug discovery is discussed...