Pengbo Zhou

Summary

Affiliation: Cornell University
Country: USA

Publications

  1. ncbi A protein knockdown strategy to study the function of beta-catenin in tumorigenesis
    Feng Cong
    Program in Cell Biology, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    BMC Mol Biol 4:10. 2003
  2. ncbi Determining protein half-lives
    Pengbo Zhou
    Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, NY, USA
    Methods Mol Biol 284:67-77. 2004
  3. ncbi Targeted protein degradation
    Pengbo Zhou
    Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Curr Opin Chem Biol 9:51-5. 2005
  4. ncbi Ectopic targeting of substrates to the ubiquitin pathway
    Jianxuan Zhang
    Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, USA
    Methods Enzymol 399:823-33. 2005
  5. ncbi REGgamma: a shortcut to destruction
    Pengbo Zhou
    Department of Pathology and Laboratory Medicine, Weill Medical College and Graduate School of Medical Sciences of Cornell University, New York, NY 10021, USA
    Cell 124:256-7. 2006
  6. ncbi Transient in utero knockout (TIUKO) of C-MYC affects late lung and intestinal development in the mouse
    J Craig Cohen
    Department of Medicine, LSU School of Medicine, 533 Bolivar St, New Orleans, USA
    BMC Dev Biol 4:4. 2004
  7. ncbi Harnessing the ubiquitination machinery to target the degradation of specific cellular proteins
    P Zhou
    Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 6:751-6. 2000
  8. ncbi DDB1 is essential for genomic stability in developing epidermis
    Yong Cang
    Howard Hughes Medical Institute and Departments of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 104:2733-7. 2007
  9. ncbi Merlin/NF2 functions upstream of the nuclear E3 ubiquitin ligase CRL4DCAF1 to suppress oncogenic gene expression
    Jonathan Cooper
    Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Sci Signal 4:pt6. 2011
  10. ncbi CUL4A abrogation augments DNA damage response and protection against skin carcinogenesis
    Liren Liu
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY 10065, USA
    Mol Cell 34:451-60. 2009

Research Grants

  1. Protein Knockout Technology/Molecular Analysis of Cancer
    Pengbo Zhou; Fiscal Year: 2004
  2. Ubiquitin-Proteolytic Control of HOXA9 in Leukemogenesis
    Pengbo Zhou; Fiscal Year: 2010
  3. Regulation of Nucleotide Excision Repair by Proteolysis
    Pengbo Zhou; Fiscal Year: 2009
  4. Regulation of Nucleotide Excision Repair by Proteolysis
    Pengbo Zhou; Fiscal Year: 2007
  5. Ubiquitin-Proteolytic Control of HOXA9 in Leukemogenesis
    Pengbo Zhou; Fiscal Year: 2007
  6. Regulation of Nucleotide Excision Repair by Proteolysis
    Pengbo Zhou; Fiscal Year: 2010

Collaborators

  • Liang Ma
  • Yue Zhang
  • Wei Li
  • Stephen Goff
  • Paul Tempst
  • Malcolm A S Moore
  • Baojie Li
  • Feng Cong
  • William Pao
  • Arianna L Kim
  • Jennifer Lee
  • Jianxuan Zhang
  • Liren Liu
  • Yong Cang
  • Jeffrey Hannah
  • Jonathan Cooper
  • Yan Yin
  • Sally A Nicholas
  • Xiaoai Chen
  • Ki Y Chung
  • Ning Zheng
  • J Craig Cohen
  • Yuewei Li
  • Lisa Prevedel
  • ELIZABETH H LACY
  • Lu Zhou
  • C Oliver Hanemann
  • Stephen B Long
  • Ryohei Ishii
  • Gaia Schiavon
  • Angela Pepe-Caprio
  • Hediye Erdjument-Bromage
  • Filippo G Giancotti
  • Liru You
  • Marco Giovannini
  • Sharrell Lee
  • Sara B Peters
  • Andrew Koff
  • Jan Jacob Schuringa
  • Jayson Bastien
  • Giovanni Morrone
  • Jae-Hung Shieh
  • Jae Hung Shieh
  • James M Ford
  • Patrick S Lin
  • Magdalena Plasilova
  • James Miller
  • Janet E Larson
  • Donald K Scott

Detail Information

Publications23

  1. ncbi A protein knockdown strategy to study the function of beta-catenin in tumorigenesis
    Feng Cong
    Program in Cell Biology, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    BMC Mol Biol 4:10. 2003
    ..Aberrant Wnt signaling, which results from mutations of either beta-catenin or adenomatous polyposis coli (APC), renders beta-catenin resistant to degradation, and has been associated with multiple types of human cancers...
  2. ncbi Determining protein half-lives
    Pengbo Zhou
    Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, NY, USA
    Methods Mol Biol 284:67-77. 2004
    ..This chapter describes in detail the two most frequently used methods, pulse-chase analysis and cycloheximide blocking, to determine a protein's half-life in yeast and cultured mammalian cells...
  3. ncbi Targeted protein degradation
    Pengbo Zhou
    Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Curr Opin Chem Biol 9:51-5. 2005
    ..It also sheds light on novel therapeutic strategies for the amelioration of human disease...
  4. ncbi Ectopic targeting of substrates to the ubiquitin pathway
    Jianxuan Zhang
    Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, USA
    Methods Enzymol 399:823-33. 2005
    ....
  5. ncbi REGgamma: a shortcut to destruction
    Pengbo Zhou
    Department of Pathology and Laboratory Medicine, Weill Medical College and Graduate School of Medical Sciences of Cornell University, New York, NY 10021, USA
    Cell 124:256-7. 2006
    ..In this issue of Cell, challenge this notion by revealing that the proteasomal activator REGgamma directs degradation of the steroid receptor coactivator SRC-3 by the 20S proteasome in an ATP- and ubiquitin-independent manner...
  6. ncbi Transient in utero knockout (TIUKO) of C-MYC affects late lung and intestinal development in the mouse
    J Craig Cohen
    Department of Medicine, LSU School of Medicine, 533 Bolivar St, New Orleans, USA
    BMC Dev Biol 4:4. 2004
    ..In utero delivery of transgenes was shown previously to result in high efficiency transfer to pulmonary and intestinal epithelial stem cells. Thus, this technology can be used to evaluate late gestation development...
  7. ncbi Harnessing the ubiquitination machinery to target the degradation of specific cellular proteins
    P Zhou
    Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 6:751-6. 2000
    ..Here we report successful engineering of the substrate receptor of a major ubiquitin-proteolytic machinery to direct the degradation of otherwise stable cellular proteins both in yeast and in mammalian cells...
  8. ncbi DDB1 is essential for genomic stability in developing epidermis
    Yong Cang
    Howard Hughes Medical Institute and Departments of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 104:2733-7. 2007
    ..Our results suggest that DDB1 plays an important role in development by controlling levels of cell cycle regulators and thereby maintaining genomic stability...
  9. ncbi Merlin/NF2 functions upstream of the nuclear E3 ubiquitin ligase CRL4DCAF1 to suppress oncogenic gene expression
    Jonathan Cooper
    Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Sci Signal 4:pt6. 2011
    ..We propose that Merlin mediates contact inhibition and suppresses tumorigenesis by translocating to the nucleus to inhibit CRL4(DCAF1)...
  10. ncbi CUL4A abrogation augments DNA damage response and protection against skin carcinogenesis
    Liren Liu
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY 10065, USA
    Mol Cell 34:451-60. 2009
    ....
  11. ncbi DCAFs, the missing link of the CUL4-DDB1 ubiquitin ligase
    Jennifer Lee
    Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, NY 10021, USA
    Mol Cell 26:775-80. 2007
    ..Recent work identified a family of DDB1 and CUL4-associated factors (DCAFs) as substrate receptors, implicating a broad spectrum of cellular processes regulated by CUL4-DDB1...
  12. ncbi Essential role of the CUL4B ubiquitin ligase in extra-embryonic tissue development during mouse embryogenesis
    Liren Liu
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College and Weill Graduate School of Medical Sciences of Cornell University, New York, NY 10065, USA
    Cell Res 22:1258-69. 2012
    ..Our study offers a strategy to generate viable Cul4b-deficient mice to model the potential neuronal and behavioral deficiencies of human CUL4B XLMR patients...
  13. ncbi Enforced expression of NUP98-HOXA9 in human CD34(+) cells enhances stem cell proliferation
    Ki Y Chung
    Department of Medicine and Moore Laboratory, Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Cancer Res 66:11781-91. 2006
    ..The resulting protein stabilization may contribute to the leukemogenic activity of the fusion protein...
  14. ncbi Regulation of DNA damage response pathways by the cullin-RING ubiquitin ligases
    Jeffrey Hannah
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College and Weill Cornell Graduate School of Medical Sciences, 1300 York Avenue, New York, NY 10065, United States
    DNA Repair (Amst) 8:536-43. 2009
    ..In this review, we will discuss recent progress in delineating the roles of cullin-RING E3 ubiquitin ligases in orchestrating the cellular DNA damage response through ubiquitination of NER factors, histones, and checkpoint effectors...
  15. ncbi A kinase-independent function of c-Abl in promoting proteolytic destruction of damaged DNA binding proteins
    Xiaoai Chen
    Department of Pathology and Laboratory Medicine, Weill Medical College and Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
    Mol Cell 22:489-99. 2006
    ..These results revealed a kinase-independent function of c-Abl in a ubiquitin-proteolytic pathway that regulates the damage recognition step of nucleotide excision repair...
  16. ncbi CUL-4A stimulates ubiquitylation and degradation of the HOXA9 homeodomain protein
    Yue Zhang
    Department of Pathology and Laboratory Medicine, Weill Medical College and Graduate School of Medical Sciences of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    EMBO J 22:6057-67. 2003
    ..These results revealed a novel regulatory mechanism of hematopoiesis by ubiquitin-dependent proteolysis...
  17. ncbi Deletion of DDB1 in mouse brain and lens leads to p53-dependent elimination of proliferating cells
    Yong Cang
    Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Cell 127:929-40. 2006
    ..Our results indicate that DDB1 plays an essential role in maintaining viability and genomic integrity of dividing cells...
  18. ncbi Exploring the functional complexity of cellular proteins by protein knockout
    Jianxuan Zhang
    Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 100:14127-32. 2003
    ..This optimized protein-knockout system offers a powerful and versatile proteomic tool to dissect diverse functional properties of cellular proteins in somatic cells...
  19. ncbi Navigating the nucleotide excision repair threshold
    Liren Liu
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College and Weill Cornell Graduate School of Medical Sciences, New York, New York 10065, USA
    J Cell Physiol 224:585-9. 2010
    ....
  20. ncbi Merlin/NF2 suppresses tumorigenesis by inhibiting the E3 ubiquitin ligase CRL4(DCAF1) in the nucleus
    Wei Li
    Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Cell 140:477-90. 2010
    ..We propose that Merlin suppresses tumorigenesis by translocating to the nucleus to inhibit CRL4(DCAF1)...
  21. ncbi Pathogenic Role of the CRL4 Ubiquitin Ligase in Human Disease
    Jennifer Lee
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College and Weill Graduate School of Medical Sciences of Cornell University New York, NY, USA
    Front Oncol 2:21. 2012
    ....
  22. ncbi SETting the clock for histone H4 monomethylation
    Jennifer Lee
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY 10065, USA
    Mol Cell 40:345-6. 2010
    ..2010), Abbas et al. (2010), and Centore et al. (2010) determined that the H4K20 histone methyltransferase PR-Set7/Set8 is posttranslationally regulated by the PCNA-dependent CRL4(Cdt2) ubiquitin ligase...
  23. ncbi Cullins and cancer
    Jennifer Lee
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College and Weill Cornell Graduate School of Medical Sciences, New York, NY, USA
    Genes Cancer 1:690-9. 2010
    ..In this review, we will discuss cullin complexes and their substrates, the regulatory pathways that affect cullin activity, and the mechanisms by which cullins may facilitate or inhibit carcinogenesis...

Research Grants14

  1. Protein Knockout Technology/Molecular Analysis of Cancer
    Pengbo Zhou; Fiscal Year: 2004
    ....
  2. Ubiquitin-Proteolytic Control of HOXA9 in Leukemogenesis
    Pengbo Zhou; Fiscal Year: 2010
    ..We propose to combine the biochemical and molecular genetic approachesin Dr. Pengbo Zhou's lab and the expertise in ex vivo and in vivo hematopoieticanalysis in Dr...
  3. Regulation of Nucleotide Excision Repair by Proteolysis
    Pengbo Zhou; Fiscal Year: 2009
    ....
  4. Regulation of Nucleotide Excision Repair by Proteolysis
    Pengbo Zhou; Fiscal Year: 2007
    ..Completion of the proposed studies will also shed light on how abnormal activation of CUL- 4A contributes to tumor development. ..
  5. Ubiquitin-Proteolytic Control of HOXA9 in Leukemogenesis
    Pengbo Zhou; Fiscal Year: 2007
    ..We propose to combine the biochemical and molecular genetic approaches in Dr. Pengbo Zhou's lab and the expertise in ex vivo and in vivo hematopoietic analysis in Dr...
  6. Regulation of Nucleotide Excision Repair by Proteolysis
    Pengbo Zhou; Fiscal Year: 2010
    ....