Pengbo Zhou

Summary

Affiliation: Cornell University
Country: USA

Publications

  1. pmc A protein knockdown strategy to study the function of beta-catenin in tumorigenesis
    Feng Cong
    Program in Cell Biology, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    BMC Mol Biol 4:10. 2003
  2. ncbi request reprint Determining protein half-lives
    Pengbo Zhou
    Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, NY, USA
    Methods Mol Biol 284:67-77. 2004
  3. ncbi request reprint Targeted protein degradation
    Pengbo Zhou
    Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Curr Opin Chem Biol 9:51-5. 2005
  4. ncbi request reprint Ectopic targeting of substrates to the ubiquitin pathway
    Jianxuan Zhang
    Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, USA
    Methods Enzymol 399:823-33. 2005
  5. ncbi request reprint REGgamma: a shortcut to destruction
    Pengbo Zhou
    Department of Pathology and Laboratory Medicine, Weill Medical College and Graduate School of Medical Sciences of Cornell University, New York, NY 10021, USA
    Cell 124:256-7. 2006
  6. pmc Pathogenic Role of the CRL4 Ubiquitin Ligase in Human Disease
    Jennifer Lee
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College and Weill Graduate School of Medical Sciences of Cornell University New York, NY, USA
    Front Oncol 2:21. 2012
  7. pmc The CUL4A ubiquitin ligase is a potential therapeutic target in skin cancer and other malignancies
    Jeffrey Hannah
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College and Weill Cornell Graduate School of Medical Sciences, New York, NY 10065, USA
    Chin J Cancer 32:478-82. 2013
  8. pmc Transient in utero knockout (TIUKO) of C-MYC affects late lung and intestinal development in the mouse
    J Craig Cohen
    Department of Medicine, LSU School of Medicine, 533 Bolivar St, New Orleans, USA
    BMC Dev Biol 4:4. 2004
  9. ncbi request reprint Harnessing the ubiquitination machinery to target the degradation of specific cellular proteins
    P Zhou
    Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 6:751-6. 2000
  10. pmc DDB1 is essential for genomic stability in developing epidermis
    Yong Cang
    Howard Hughes Medical Institute and Departments of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 104:2733-7. 2007

Collaborators

Detail Information

Publications27

  1. pmc A protein knockdown strategy to study the function of beta-catenin in tumorigenesis
    Feng Cong
    Program in Cell Biology, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    BMC Mol Biol 4:10. 2003
    ..Aberrant Wnt signaling, which results from mutations of either beta-catenin or adenomatous polyposis coli (APC), renders beta-catenin resistant to degradation, and has been associated with multiple types of human cancers...
  2. ncbi request reprint Determining protein half-lives
    Pengbo Zhou
    Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, NY, USA
    Methods Mol Biol 284:67-77. 2004
    ..This chapter describes in detail the two most frequently used methods, pulse-chase analysis and cycloheximide blocking, to determine a protein's half-life in yeast and cultured mammalian cells...
  3. ncbi request reprint Targeted protein degradation
    Pengbo Zhou
    Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Curr Opin Chem Biol 9:51-5. 2005
    ..It also sheds light on novel therapeutic strategies for the amelioration of human disease...
  4. ncbi request reprint Ectopic targeting of substrates to the ubiquitin pathway
    Jianxuan Zhang
    Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, USA
    Methods Enzymol 399:823-33. 2005
    ....
  5. ncbi request reprint REGgamma: a shortcut to destruction
    Pengbo Zhou
    Department of Pathology and Laboratory Medicine, Weill Medical College and Graduate School of Medical Sciences of Cornell University, New York, NY 10021, USA
    Cell 124:256-7. 2006
    ..In this issue of Cell, challenge this notion by revealing that the proteasomal activator REGgamma directs degradation of the steroid receptor coactivator SRC-3 by the 20S proteasome in an ATP- and ubiquitin-independent manner...
  6. pmc Pathogenic Role of the CRL4 Ubiquitin Ligase in Human Disease
    Jennifer Lee
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College and Weill Graduate School of Medical Sciences of Cornell University New York, NY, USA
    Front Oncol 2:21. 2012
    ....
  7. pmc The CUL4A ubiquitin ligase is a potential therapeutic target in skin cancer and other malignancies
    Jeffrey Hannah
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College and Weill Cornell Graduate School of Medical Sciences, New York, NY 10065, USA
    Chin J Cancer 32:478-82. 2013
    ..In this review, we outline the established functions of CUL4A and discuss the E3 ligase's emergence as a potential driver of tumorigenesis. ..
  8. pmc Transient in utero knockout (TIUKO) of C-MYC affects late lung and intestinal development in the mouse
    J Craig Cohen
    Department of Medicine, LSU School of Medicine, 533 Bolivar St, New Orleans, USA
    BMC Dev Biol 4:4. 2004
    ..In utero delivery of transgenes was shown previously to result in high efficiency transfer to pulmonary and intestinal epithelial stem cells. Thus, this technology can be used to evaluate late gestation development...
  9. ncbi request reprint Harnessing the ubiquitination machinery to target the degradation of specific cellular proteins
    P Zhou
    Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 6:751-6. 2000
    ..Here we report successful engineering of the substrate receptor of a major ubiquitin-proteolytic machinery to direct the degradation of otherwise stable cellular proteins both in yeast and in mammalian cells...
  10. pmc DDB1 is essential for genomic stability in developing epidermis
    Yong Cang
    Howard Hughes Medical Institute and Departments of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 104:2733-7. 2007
    ..Our results suggest that DDB1 plays an important role in development by controlling levels of cell cycle regulators and thereby maintaining genomic stability...
  11. pmc Improved ex vivo expansion of adult hematopoietic stem cells by overcoming CUL4-mediated degradation of HOXB4
    Jennifer Lee
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY, USA
    Blood 121:4082-9. 2013
    ..Our studies demonstrate the feasibility of engineering a stable HOXB4 variant to overcome a major technical hurdle in the ex vivo expansion of adult HSCs and early progenitors for human therapeutic use...
  12. pmc CUL4A abrogation augments DNA damage response and protection against skin carcinogenesis
    Liren Liu
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY 10065, USA
    Mol Cell 34:451-60. 2009
    ....
  13. doi request reprint Merlin/NF2 functions upstream of the nuclear E3 ubiquitin ligase CRL4DCAF1 to suppress oncogenic gene expression
    Jonathan Cooper
    Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Sci Signal 4:pt6. 2011
    ..We propose that Merlin mediates contact inhibition and suppresses tumorigenesis by translocating to the nucleus to inhibit CRL4(DCAF1)...
  14. ncbi request reprint A kinase-independent function of c-Abl in promoting proteolytic destruction of damaged DNA binding proteins
    Xiaoai Chen
    Department of Pathology and Laboratory Medicine, Weill Medical College and Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
    Mol Cell 22:489-99. 2006
    ..These results revealed a kinase-independent function of c-Abl in a ubiquitin-proteolytic pathway that regulates the damage recognition step of nucleotide excision repair...
  15. ncbi request reprint DCAFs, the missing link of the CUL4-DDB1 ubiquitin ligase
    Jennifer Lee
    Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, NY 10021, USA
    Mol Cell 26:775-80. 2007
    ..Recent work identified a family of DDB1 and CUL4-associated factors (DCAFs) as substrate receptors, implicating a broad spectrum of cellular processes regulated by CUL4-DDB1...
  16. pmc Essential role of the CUL4B ubiquitin ligase in extra-embryonic tissue development during mouse embryogenesis
    Liren Liu
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College and Weill Graduate School of Medical Sciences of Cornell University, New York, NY 10065, USA
    Cell Res 22:1258-69. 2012
    ..Our study offers a strategy to generate viable Cul4b-deficient mice to model the potential neuronal and behavioral deficiencies of human CUL4B XLMR patients...
  17. ncbi request reprint Enforced expression of NUP98-HOXA9 in human CD34(+) cells enhances stem cell proliferation
    Ki Y Chung
    Department of Medicine and Moore Laboratory, Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Cancer Res 66:11781-91. 2006
    ..The resulting protein stabilization may contribute to the leukemogenic activity of the fusion protein...
  18. pmc Regulation of DNA damage response pathways by the cullin-RING ubiquitin ligases
    Jeffrey Hannah
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College and Weill Cornell Graduate School of Medical Sciences, 1300 York Avenue, New York, NY 10065, United States
    DNA Repair (Amst) 8:536-43. 2009
    ..In this review, we will discuss recent progress in delineating the roles of cullin-RING E3 ubiquitin ligases in orchestrating the cellular DNA damage response through ubiquitination of NER factors, histones, and checkpoint effectors...
  19. ncbi request reprint Deletion of DDB1 in mouse brain and lens leads to p53-dependent elimination of proliferating cells
    Yong Cang
    Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Cell 127:929-40. 2006
    ..Our results indicate that DDB1 plays an essential role in maintaining viability and genomic integrity of dividing cells...
  20. pmc CUL-4A stimulates ubiquitylation and degradation of the HOXA9 homeodomain protein
    Yue Zhang
    Department of Pathology and Laboratory Medicine, Weill Medical College and Graduate School of Medical Sciences of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    EMBO J 22:6057-67. 2003
    ..These results revealed a novel regulatory mechanism of hematopoiesis by ubiquitin-dependent proteolysis...
  21. pmc Merlin/NF2 Loss-Driven Tumorigenesis Linked to CRL4(DCAF1)-Mediated Inhibition of the Hippo Pathway Kinases Lats1 and 2 in the Nucleus
    Wei Li
    Cell Biology Program, Sloan Kettering Institute for Cancer Research, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA Electronic address
    Cancer Cell 26:48-60. 2014
    ..Analysis of clinical samples confirms that this pathway operates in NF2-mutant tumors. We conclude that derepressed CRL4(DCAF1) promotes activation of YAP by inhibiting Lats1 and 2 in the nucleus. ..
  22. pmc Navigating the nucleotide excision repair threshold
    Liren Liu
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College and Weill Cornell Graduate School of Medical Sciences, New York, New York 10065, USA
    J Cell Physiol 224:585-9. 2010
    ....
  23. pmc Exploring the functional complexity of cellular proteins by protein knockout
    Jianxuan Zhang
    Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 100:14127-32. 2003
    ..This optimized protein-knockout system offers a powerful and versatile proteomic tool to dissect diverse functional properties of cellular proteins in somatic cells...
  24. pmc Merlin/NF2 suppresses tumorigenesis by inhibiting the E3 ubiquitin ligase CRL4(DCAF1) in the nucleus
    Wei Li
    Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Cell 140:477-90. 2010
    ..We propose that Merlin suppresses tumorigenesis by translocating to the nucleus to inhibit CRL4(DCAF1)...
  25. ncbi request reprint Axl receptor tyrosine kinase expression in breast cancer
    Timothy M D'Alfonso
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, USA
    J Clin Pathol 67:690-6. 2014
    ....
  26. pmc SETting the clock for histone H4 monomethylation
    Jennifer Lee
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY 10065, USA
    Mol Cell 40:345-6. 2010
    ..2010), Abbas et al. (2010), and Centore et al. (2010) determined that the H4K20 histone methyltransferase PR-Set7/Set8 is posttranslationally regulated by the PCNA-dependent CRL4(Cdt2) ubiquitin ligase...
  27. pmc Cullins and cancer
    Jennifer Lee
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College and Weill Cornell Graduate School of Medical Sciences, New York, NY, USA
    Genes Cancer 1:690-9. 2010
    ..In this review, we will discuss cullin complexes and their substrates, the regulatory pathways that affect cullin activity, and the mechanisms by which cullins may facilitate or inhibit carcinogenesis...

Research Grants14

  1. Protein Knockout Technology/Molecular Analysis of Cancer
    Pengbo Zhou; Fiscal Year: 2004
    ....
  2. Regulation of Nucleotide Excision Repair by Proteolysis
    Pengbo Zhou; Fiscal Year: 2003
    ..Completion of the proposed studies will also shed light on how abnormal activation of CUL- 4A contributes to tumor development. ..
  3. Ubiquitin-Proteolytic Control of HOXA9 in Leukemogenesis
    Pengbo Zhou; Fiscal Year: 2010
    ..We propose to combine the biochemical and molecular genetic approachesin Dr. Pengbo Zhou's lab and the expertise in ex vivo and in vivo hematopoieticanalysis in Dr...
  4. Regulation of Nucleotide Excision Repair by Proteolysis
    Pengbo Zhou; Fiscal Year: 2010
    ....
  5. Regulation of Nucleotide Excision Repair by Proteolysis
    Pengbo Zhou; Fiscal Year: 2006
    ..Completion of the proposed studies will also shed light on how abnormal activation of CUL- 4A contributes to tumor development. ..
  6. Ubiquitin-Proteolytic Control of HOXA9 in Leukemogenesis
    Pengbo Zhou; Fiscal Year: 2006
    ..We propose to combine the biochemical and molecular genetic approaches in Dr. Pengbo Zhou's lab and the expertise in ex vivo and in vivo hematopoietic analysis in Dr...
  7. Regulation of Nucleotide Excision Repair by Proteolysis
    Pengbo Zhou; Fiscal Year: 2005
    ..Completion of the proposed studies will also shed light on how abnormal activation of CUL- 4A contributes to tumor development. ..
  8. Regulation of Nucleotide Excision Repair by Proteolysis
    Pengbo Zhou; Fiscal Year: 2004
    ..Completion of the proposed studies will also shed light on how abnormal activation of CUL- 4A contributes to tumor development. ..
  9. Ubiquitin-Proteolytic Control of HOXA9 in Leukemogenesis
    Pengbo Zhou; Fiscal Year: 2007
    ..We propose to combine the biochemical and molecular genetic approaches in Dr. Pengbo Zhou's lab and the expertise in ex vivo and in vivo hematopoietic analysis in Dr...
  10. Ubiquitin-Proteolytic Control of HOXA9 in Leukemogenesis
    Pengbo Zhou; Fiscal Year: 2009
    ..We propose to combine the biochemical and molecular genetic approachesin Dr. Pengbo Zhou's lab and the expertise in ex vivo and in vivo hematopoieticanalysis in Dr...
  11. Regulation of Nucleotide Excision Repair by Proteolysis
    Pengbo Zhou; Fiscal Year: 2009
    ....
  12. Regulation of Nucleotide Excision Repair by Proteolysis
    Pengbo Zhou; Fiscal Year: 2007
    ..Completion of the proposed studies will also shed light on how abnormal activation of CUL- 4A contributes to tumor development. ..