Hao Wu

Summary

Affiliation: Cornell University
Country: USA

Publications

  1. pmc Crystal structure of inhibitor of κB kinase β
    Guozhou Xu
    Department of Biochemistry, Weill Cornell Medical College, New York, New York 10021, USA
    Nature 472:325-30. 2011
  2. pmc The Fas-FADD death domain complex structure reveals the basis of DISC assembly and disease mutations
    Liwei Wang
    Department of Biochemistry, Weill Cornell Medical College, New York, New York, USA
    Nat Struct Mol Biol 17:1324-9. 2010
  3. pmc Smac mimetics and TNFalpha: a dangerous liaison?
    Hao Wu
    Department of Biochemistry, Weill Medical College of Cornell University, New York, NY 10021, USA
    Cell 131:655-8. 2007
  4. ncbi request reprint TRAF6, a molecular bridge spanning adaptive immunity, innate immunity and osteoimmunology
    Hao Wu
    Department of Biochemistry, Weill Medical College of Cornell University, New York, NY 10021, USA
    Bioessays 25:1096-105. 2003
  5. pmc Molecular basis for the unique deubiquitinating activity of the NF-kappaB inhibitor A20
    Su Chang Lin
    Department of Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    J Mol Biol 376:526-40. 2008
  6. pmc Structural basis for recognition of diubiquitins by NEMO
    Yu Chih Lo
    Department of Biochemistry, Weill Cornell Medical College, New York, NY 10021, USA
    Mol Cell 33:602-15. 2009
  7. pmc XIAP induces NF-kappaB activation via the BIR1/TAB1 interaction and BIR1 dimerization
    Miao Lu
    Department of Biochemistry, Weill Medical College of Cornell University, New York, NY 10021, USA
    Mol Cell 26:689-702. 2007
  8. pmc Caspase-9 holoenzyme is a specific and optimal procaspase-3 processing machine
    Qian Yin
    Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 10021, USA
    Mol Cell 22:259-68. 2006
  9. pmc Crystal structure of the BIR1 domain of XIAP in two crystal forms
    Su Chang Lin
    Department of Biochemistry, Weill Medical College of Cornell University, New York, NY 10021, USA
    J Mol Biol 372:847-54. 2007
  10. pmc E2 interaction and dimerization in the crystal structure of TRAF6
    Qian Yin
    Weill Medical College of Cornell University, New York, New York, USA
    Nat Struct Mol Biol 16:658-66. 2009

Detail Information

Publications73

  1. pmc Crystal structure of inhibitor of κB kinase β
    Guozhou Xu
    Department of Biochemistry, Weill Cornell Medical College, New York, New York 10021, USA
    Nature 472:325-30. 2011
    ..Other IKK family members, IKKα, TBK1 and IKK-i, may have a similar trimodular architecture and function...
  2. pmc The Fas-FADD death domain complex structure reveals the basis of DISC assembly and disease mutations
    Liwei Wang
    Department of Biochemistry, Weill Cornell Medical College, New York, New York, USA
    Nat Struct Mol Biol 17:1324-9. 2010
    ..The structure optimally positions the FADD death effector domain (DED) to interact with the caspase-8 DED for caspase recruitment and higher-order aggregation...
  3. pmc Smac mimetics and TNFalpha: a dangerous liaison?
    Hao Wu
    Department of Biochemistry, Weill Medical College of Cornell University, New York, NY 10021, USA
    Cell 131:655-8. 2007
    ..2007), now report that Smac mimetics primarily kill cancer cells via a different mechanism, the induction of autoubiquitination and degradation of cIAPs, which culminates in TNFalpha-mediated cell death...
  4. ncbi request reprint TRAF6, a molecular bridge spanning adaptive immunity, innate immunity and osteoimmunology
    Hao Wu
    Department of Biochemistry, Weill Medical College of Cornell University, New York, NY 10021, USA
    Bioessays 25:1096-105. 2003
    ..By virtue of its many signaling roles, TRAF6 represents an important target in the regulation of many disease processes, including immunity, inflammation and osteoporosis...
  5. pmc Molecular basis for the unique deubiquitinating activity of the NF-kappaB inhibitor A20
    Su Chang Lin
    Department of Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    J Mol Biol 376:526-40. 2008
    ....
  6. pmc Structural basis for recognition of diubiquitins by NEMO
    Yu Chih Lo
    Department of Biochemistry, Weill Cornell Medical College, New York, NY 10021, USA
    Mol Cell 33:602-15. 2009
    ..These studies uncover the energetics and geometry for mutual recognition of NEMO and diubiquitins...
  7. pmc XIAP induces NF-kappaB activation via the BIR1/TAB1 interaction and BIR1 dimerization
    Miao Lu
    Department of Biochemistry, Weill Medical College of Cornell University, New York, NY 10021, USA
    Mol Cell 26:689-702. 2007
    ..Disruption of BIR1 dimerization abolishes XIAP-mediated NF-kappaB activation, implicating a proximity-induced mechanism for TAK1 activation...
  8. pmc Caspase-9 holoenzyme is a specific and optimal procaspase-3 processing machine
    Qian Yin
    Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 10021, USA
    Mol Cell 22:259-68. 2006
    ..Therefore, in addition to dimerization, the apoptosome activates caspase-9 by enhancing its affinity for procaspase-3, which is important for procaspase-3 activation at the physiological concentration...
  9. pmc Crystal structure of the BIR1 domain of XIAP in two crystal forms
    Su Chang Lin
    Department of Biochemistry, Weill Medical College of Cornell University, New York, NY 10021, USA
    J Mol Biol 372:847-54. 2007
    ....
  10. pmc E2 interaction and dimerization in the crystal structure of TRAF6
    Qian Yin
    Weill Medical College of Cornell University, New York, New York, USA
    Nat Struct Mol Biol 16:658-66. 2009
    ..The mismatch of dimeric and trimeric symmetry may provide a mode of infinite oligomerization that facilitates ligand-dependent signal transduction of many immune receptors...
  11. pmc Crystal structure of MC159 reveals molecular mechanism of DISC assembly and FLIP inhibition
    Jin Kuk Yang
    Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 10021, USA
    Mol Cell 20:939-49. 2005
    ..This interaction apparently competes with FADD self-association and disrupts higher-order oligomerization required for caspase activation in the DISC...
  12. pmc A novel mechanism for adenylyl cyclase inhibition from the crystal structure of its complex with catechol estrogen
    Clemens Steegborn
    Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 10021, USA
    J Biol Chem 280:31754-9. 2005
    ..This novel inhibition mechanism likely applies to other adenylyl cyclase inhibitors, and the identified ligand-binding site has important implications for the development of specific adenylyl cyclase inhibitors...
  13. pmc Crystal structures of the TRAF2: cIAP2 and the TRAF1: TRAF2: cIAP2 complexes: affinity, specificity, and regulation
    Chao Zheng
    Weill Medical College, Cornell University, New York, NY 10021, USA
    Mol Cell 38:101-13. 2010
    ..Because TRAF1 is upregulated by many stimuli, it may modulate the interaction of TRAF2 with cIAP1/2, which explains regulatory roles of TRAF1 in TNF signaling...
  14. ncbi request reprint Distinct molecular mechanism for initiating TRAF6 signalling
    Hong Ye
    Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 10021, USA
    Nature 418:443-7. 2002
    ..Our studies identify a universal mechanism by which TRAF6 regulates several signalling cascades in adaptive immunity, innate immunity and bone homeostasis...
  15. pmc High-affinity interaction between IKKbeta and NEMO
    Yu Chih Lo
    Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 10021, USA
    Biochemistry 47:3109-16. 2008
    ..Biochemical characterization using multiangle light scattering (MALS) coupled with refractive index shows that the longer IKKbeta C-terminal region forms a 2:2 stoichiometirc complex with NEMO...
  16. pmc A dimeric Smac/diablo peptide directly relieves caspase-3 inhibition by XIAP. Dynamic and cooperative regulation of XIAP by Smac/Diablo
    Zhonghua Gao
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    J Biol Chem 282:30718-27. 2007
    ..We also show that XIAP homotrimerizes via its C-terminal Ring domain, making its inhibitory activity toward caspase-3 more susceptible to Smac...
  17. pmc Crystal structure of RAIDD death domain implicates potential mechanism of PIDDosome assembly
    Hyun Ho Park
    Department of Biochemistry, Weill Medical College and Graduate School of Medical Sciences of Cornell University, New York, NY 10021, USA
    J Mol Biol 357:358-64. 2006
    ..0 A resolution. The high-resolution structure reveals important features of RAIDD DD that may be important for DD folding and dynamics and for assembly of the PIDDosome...
  18. pmc Structural basis for the lack of E2 interaction in the RING domain of TRAF2
    Qian Yin
    Weill Medical College of Cornell University, New York, New York 10021, USA
    Biochemistry 48:10558-67. 2009
    ..Our structural observation should prompt a re-evaluation of the role of TRAF2 in TNFalpha signaling and may indicate that TRAF2-associated proteins such as cIAPs may be the ubiquitin ligases for NF-kappaB signaling...
  19. pmc Mapping of antigenic sites on the nucleocapsid protein of the severe acute respiratory syndrome coronavirus
    Yuxian He
    Lindsley F Kimball Research Institute, New York Blood Center, New York, NY 10021, USA
    J Clin Microbiol 42:5309-14. 2004
    ..These results suggest that the above antigenic sites on the N protein are important in eliciting humoral immune response against SARS-CoV in humans and animals and can be used as antigens for developing diagnostic tests...
  20. ncbi request reprint Crystal structure of the putative adapter protein MTH1859
    Hong Ye
    Department of Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    J Struct Biol 148:251-6. 2004
    ..These interactions lead to the formation of a honeycomb structure and suggest that the family of MTH1859-like proteins might function as adapters for protein complex assembly...
  21. pmc Death domain assembly mechanism revealed by crystal structure of the oligomeric PIDDosome core complex
    Hyun Ho Park
    Weill Medical College, Graduate School of Medical Sciences of Cornell University, New York, NY 10021, USA
    Cell 128:533-46. 2007
    ..The three types of interactions may represent most, if not all, modes of interactions in the DD superfamily for assembling complexes of different stoichiometry...
  22. ncbi request reprint Requirement of both the second and third BIR domains for the relief of X-linked inhibitor of apoptosis protein (XIAP)-mediated caspase inhibition by Smac
    Yihua Huang
    Department of Biochemistry, Weill Medical College and Graduate School of Medical Sciences of Cornell University, New York, NY 10021, USA
    J Biol Chem 278:49517-22. 2003
    ....
  23. ncbi request reprint Antagonizing XIAP-mediated caspase-3 inhibition. Achilles' heel of cancers?
    Yihua Huang
    Department of Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Cancer Cell 5:1-2. 2004
    ....
  24. pmc The death domain superfamily in intracellular signaling of apoptosis and inflammation
    Hyun Ho Park
    Department of Biochemistry, Weill Medical College and Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
    Annu Rev Immunol 25:561-86. 2007
    ..By analyzing the potential molecular basis for the function of these domains, we hope to provide a comprehensive understanding of the function, structure, interaction, and evolution of this important family of domains...
  25. pmc A class of allosteric caspase inhibitors identified by high-throughput screening
    Taya Feldman
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Mol Cell 47:585-95. 2012
    ..Based on these kinetic, biochemical, and structural analyses, we suggest that these compounds are allosteric caspase inhibitors that function through binding to the dimerization interface of caspases...
  26. pmc PHAPI/pp32 suppresses tumorigenesis by stimulating apoptosis
    Wei Pan
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 284:6946-54. 2009
    ..Disruption of the nuclear localization signal of PHAPI caused a modest decrease of its tumor-suppressive function, indicating that nuclear localization of PHAPI contributes to, but is not essential for, tumor suppression...
  27. pmc Cyclic di-GMP sensing via the innate immune signaling protein STING
    Qian Yin
    Department of Biochemistry, Weill Cornell Medical College, New York, NY 10065, USA
    Mol Cell 46:735-45. 2012
    ..The structures provide a remarkable example of pathogen-host interactions in which a unique microbial molecule directly engages the innate immune system...
  28. pmc Crystallization and preliminary X-ray crystallographic studies of Drep-3, a DFF-related protein from Drosophila melanogaster
    Hyun Ho Park
    Department of Biochemistry, Weill Medical College and Graduate School of Medical Sciences of Cornell University, New York, NY 10021, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:597-9. 2006
    ..8 and 3.0 A resolution, respectively. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 56.9, b = 125.4, c = 168.7 A. The asymmetric unit is estimated to contain one homotetramer...
  29. ncbi request reprint The use of construct variation and diffraction data analysis in the crystallization of the TRAF domain of human tumor necrosis factor receptor associated factor 6
    Hong Ye
    Department of Biochemistry, Weill Medical College of Cornell University, New York, NY 10021, USA
    Acta Crystallogr D Biol Crystallogr 58:1886-8. 2002
    ..Here, it is shown that construct variation can be an efficient strategy in expressing soluble proteins from bacteria and that clues from poor crystals may be used to improve crystal packing and to optimize crystal quality...
  30. pmc The KSHV oncoprotein vFLIP contains a TRAF-interacting motif and requires TRAF2 and TRAF3 for signalling
    Ilaria Guasparri
    Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, New York 10021, USA
    EMBO Rep 7:114-9. 2006
    ..TRAF2, but not TRAF3, mediates the association of vFLIP with the IkappaB kinase complex. These data indicate that vFLIP uses TRAF2 and TRAF3 for signalling to NF-kappaB, which is crucial for KSHV-associated lymphomagenesis...
  31. pmc Bicarbonate activation of adenylyl cyclase via promotion of catalytic active site closure and metal recruitment
    Clemens Steegborn
    Department of Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, New York 10021, USA
    Nat Struct Mol Biol 12:32-7. 2005
    ..The mechanisms of calcium and bicarbonate sensing define a reaction pathway involving active site closure and metal recruitment that may be universal for class III cyclases...
  32. ncbi request reprint ABAD directly links Abeta to mitochondrial toxicity in Alzheimer's disease
    Joyce W Lustbader
    Center for Reproductive Sciences and Department of Obstetrics and Gynecology, College of Physicians and Surgeons, Columbia University, 630 West 168th Street, New York, NY 10032, USA
    Science 304:448-52. 2004
    ..Transgenic mice overexpressing ABAD in an Abeta-rich environment manifest exaggerated neuronal oxidative stress and impaired memory. These data suggest that the ABAD-Abeta interaction may be a therapeutic target in AD...
  33. pmc Crystallization and preliminary X-ray crystallographic studies of the oligomeric death-domain complex between PIDD and RAIDD
    Hyun Ho Park
    Department of Biochemistry, Weill Medical College and Graduate School of Medical Sciences of Cornell University, New York, NY 10021, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:229-32. 2007
    ..X-ray diffraction data were collected to resolutions of 3.2 and 4.0 A from a native and a Hg-derivative crystal, respectively. The crystals belong to space group P6(5), with unit-cell parameters a = b = 138.4, c = 207.6 A...
  34. ncbi request reprint Native chemical ligation in covalent caspase inhibition by p35
    Miao Lu
    Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 10021, USA
    Chem Biol 13:117-22. 2006
    ..Participation of native chemical ligation in caspase inhibition by p35 illustrates an unusual mechanism of protease inhibition...
  35. ncbi request reprint Molecular basis for the unique specificity of TRAF6
    Jee Y Chung
    Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 10021, USA
    Adv Exp Med Biol 597:122-30. 2007
    ..This chapter will highlight the structural and biochemical studies of TRAF6 in receptor interactions and discuss the potential for peptidomimetic drug application based on TRAF6 receptor binding motif...
  36. ncbi request reprint Assembly of post-receptor signaling complexes for the tumor necrosis factor receptor superfamily
    Hao Wu
    Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 10021, USA
    Adv Protein Chem 68:225-79. 2004
    ..This review summarizes recent structural, biochemical, and functional studies of these signal transducers and proposes the molecular mechanisms of the intracellular signal transduction...
  37. ncbi request reprint Structural revelations of TRAF2 function in TNF receptor signaling pathway
    Jee Y Chung
    Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 10021, USA
    Adv Exp Med Biol 597:93-113. 2007
    ..There are currently six canonical mammalian TRAFs. This review will focus on the unique structural features of TRAF2 protein and its role in cell survival signaling...
  38. pmc Crystal structure and versatile functional roles of the COP9 signalosome subunit 1
    Jung Hoon Lee
    Department of Biochemistry, Weill Cornell Medical College, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 110:11845-50. 2013
    ..Therefore, the CSN complex uses multiple mechanisms to hinder NF-κB activation, a principle likely to hold true for its regulation of many other targets and pathways. ..
  39. pmc Structural studies of NF-κB signaling
    Chao Zheng
    Department of Biochemistry, Weill Cornell Medical College, New York, NY 10021, USA
    Cell Res 21:183-95. 2011
    ..2) How do IκBs inhibit NF-κB activities in the steady state? (3) How do upstream signaling molecules activate the NF-κB pathway? and (4) How do the feedback loops shut down the NF-κB pathway to avoid constitutive NF-κB activation?..
  40. ncbi request reprint Identification of immunodominant sites on the spike protein of severe acute respiratory syndrome (SARS) coronavirus: implication for developing SARS diagnostics and vaccines
    Yuxian He
    Lindsley F Kimball Research Institute, New York Blood Center, New York, NY 10021, USA
    J Immunol 173:4050-7. 2004
    ..This study also provides information useful for designing SARS vaccines and understanding the SARS pathogenesis...
  41. pmc Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling
    Su Chang Lin
    Department of Biochemistry, Weill Cornell Medical College, New York, New York 10021, USA
    Nature 465:885-90. 2010
    ..The MyD88-IRAK4-IRAK2 complex provides a template for Toll signalling in Drosophila and an elegant mechanism for versatile assembly and regulation of DD complexes in signal transduction...
  42. ncbi request reprint DNA binding is required for the apoptogenic action of apoptosis inducing factor
    Hong Ye
    Department of Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, New York 10021, USA
    Nat Struct Biol 9:680-4. 2002
    ..The potential DNA-binding site identified from mutagenesis also coincides with computational docking of a DNA duplex. These observations suggest that AIF-induced nuclear apoptosis requires a direct interaction with DNA...
  43. pmc MALT1 small molecule inhibitors specifically suppress ABC-DLBCL in vitro and in vivo
    Lorena Fontan
    Division of Hematology and Medical Oncology, Weill Cornell Medical College, New York, NY 10021, USA
    Cancer Cell 22:812-24. 2012
    ..The compound was also effective against primary human non-germinal center B cell-like DLBCLs ex vivo...
  44. pmc Structural insights into the assembly of large oligomeric signalosomes in the Toll-like receptor-interleukin-1 receptor superfamily
    Ryan Ferrao
    Department of Biochemistry, Weill Cornell Medical College, New York, NY 10021, USA
    Sci Signal 5:re3. 2012
    ..Formation of large oligomeric signalosomes may help to establish a digital threshold response in TLR and IL-1R signaling...
  45. pmc The RIP1/RIP3 necrosome forms a functional amyloid signaling complex required for programmed necrosis
    Jixi Li
    Department of Biochemistry, Weill Cornell Medical College, New York, NY 10065, USA
    Cell 150:339-50. 2012
    ..The current study provides insight into the structural changes that occur when RIP kinases are triggered to execute different signaling outcomes and expands the realm of amyloids to complex formation and signaling...
  46. pmc Helical assembly in the death domain (DD) superfamily
    Ryan Ferrao
    Department of Biochemistry, Weill Cornell Medical College and Graduate School of Medical Sciences, New York, NY 10021, USA
    Curr Opin Struct Biol 22:241-7. 2012
    ..This review will focus on how the three DD interaction surfaces cooperate to facilitate the assembly of these oligomeric signaling complexes...
  47. pmc The CBM signalosome: potential therapeutic target for aggressive lymphoma?
    Chenghua Yang
    Joint Center for Translational Research of Chronic Diseases, Changhai Hospital, The Second Military Medical University, Shanghai 2000433, China Department of Molecular Pharmacology and Chemistry, Sloan Kettering Institute, New York, NY 10021, USA Electronic address
    Cytokine Growth Factor Rev 25:175-83. 2014
    ..Here, we will highlight these progresses and give an outlook on the potential translation of this knowledge from bench to bedside for aggressive lymphoma patients. ..
  48. ncbi request reprint Mutational analyses of the p35-caspase interaction. A bowstring kinetic model of caspase inhibition by p35
    Guozhou Xu
    Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 10021, USA
    J Biol Chem 278:5455-61. 2003
    ..A bowstring kinetic model for p35 function is derived in which the tension generated in the bowstring system during the pre-cleavage interaction is crucial for the fast post-cleavage conformational changes required for inhibition...
  49. ncbi request reprint General co-expression vectors for the overexpression of heterodimeric protein complexes in Escherichia coli
    Oki K Dzivenu
    Department of Biochemistry, Weill Medical College, Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Protein Expr Purif 38:1-8. 2004
    ..coli for detailed biochemical and structural studies...
  50. pmc Differential contribution of basic residues to HIV-1 nucleocapsid protein's nucleic acid chaperone function and retroviral replication
    Hao Wu
    Department of Physics, Northeastern University, Boston, MA 02115, USA, Department of Chemistry and Biochemistry, Center for RNA Biology, and Center for Retrovirus Research, The Ohio State University, Columbus, OH 43210, USA, AIDS and Cancer Virus Program, Leidos Biomedical Research, Inc, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA and Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Nucleic Acids Res 42:2525-37. 2014
    ..Although NC's aromatic residues participate directly in DNA intercalation, our findings suggest that specific basic residues enhance these interactions, resulting in optimal NA chaperone activity. ..
  51. pmc Molecular basis of NF-κB signaling
    Johanna Napetschnig
    Department of Biochemistry, Weill Cornell Medical College, New York, New York 10021, USA
    Annu Rev Biophys 42:443-68. 2013
    ..Although these structures only provide snapshots of isolated processes, an emerging picture is that these signaling cascades coalesce into large oligomeric signaling complexes, or signalosomes, for signal propagation...
  52. ncbi request reprint All TRAFs are not created equal: common and distinct molecular mechanisms of TRAF-mediated signal transduction
    Jee Y Chung
    Department of Biochemistry, Weill Medical College of Cornell University, New York, NY 10021, USA
    J Cell Sci 115:679-88. 2002
    ..We raise additional questions and propose hypotheses regarding the molecular basis of TRAF signaling specificity...
  53. pmc TAK1-dependent signaling requires functional interaction with TAB2/TAB3
    Arnaud Besse
    Department of Experimental Therapeutics, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    J Biol Chem 282:3918-28. 2007
    ..Significantly, our study provides evidence that the TAB2/TAB3 interaction with TAK1 is crucial for the activation of signaling cascades mediated by IL-1, TNF, and RANKL...
  54. pmc Site-specific Lys-63-linked tumor necrosis factor receptor-associated factor 6 auto-ubiquitination is a critical determinant of I kappa B kinase activation
    Betty Lamothe
    Department of Experimental Therapeutics, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    J Biol Chem 282:4102-12. 2007
    ..These data establish a signaling cascade in which regulated site-specific Lys-63-linked TRAF6 auto-ubiquitination is the critical upstream mediator of IKK...
  55. pmc Identification of the yeast R-SNARE Nyv1p as a novel longin domain-containing protein
    Wenyu Wen
    Department of Biochemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, Special Administrative Region of China
    Mol Biol Cell 17:4282-99. 2006
    ....
  56. ncbi request reprint CD4 dimers constitute the functional component required for T cell activation
    Maria Cristina Moldovan
    Laboratoire d Immunologie, Institut de Recherches Cliniques de Montreal, Quebec, Canada
    J Immunol 169:6261-8. 2002
    ..More importantly, we demonstrate that dimer formation is essential for the coligand and coreceptor functions of CD4 in T cell activation. These data strongly suggest that CD4 dimerization is necessary for helper T cell function...
  57. ncbi request reprint [The alteration of inflammatory cytokine during acute pancreatitis]
    Xiaoli Chen
    Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China
    Hua Xi Yi Ke Da Xue Xue Bao 33:238-40, 243. 2002
    ....
  58. pmc Cytoplasmic male sterility of rice with boro II cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing
    Zhonghua Wang
    Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Province, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
    Plant Cell 18:676-87. 2006
    ..In the presence of both restorers, RF1A was epistatic over RF1B in the mRNA processing. We have also shown that RF1A plays an additional role in promoting the editing of atp6 mRNAs, independent of its cleavage function...
  59. ncbi request reprint Elongation factor-2 kinase: its role in protein synthesis and autophagy
    William N Hait
    Department of Pharmacology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 195 Little Albany Street, New Brunswick, New Jersey 08901, USA
    Autophagy 2:294-6. 2006
    ..Therefore, eEF-2 kinase activation may be a part of a survival mechanism in glioblastoma, and targeting this kinase may represent a novel approach to cancer treatment...
  60. ncbi request reprint Triptolide, a diterpenoid triepoxide, suppresses inflammation and cartilage destruction in collagen-induced arthritis mice
    Na Lin
    Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No 16, Nanxiaojie, Dongzhimennei, Beijing 100700, China
    Biochem Pharmacol 73:136-46. 2007
    ..These results suggest that triptolide exerts novel chondroprotective and anti-inflammatory effects on RA, and the therapeutic action of TWHF on RA is, in part, due to the triptolide activities...
  61. ncbi request reprint Effects of salvianolic acid a on oxidative stress and liver injury induced by carbon tetrachloride in rats
    Zhi ming Wu
    Division of Hepatitis Institute, Beijing You an Hospital Affiliated with Capital University of Medical Sciences, Beijing, China
    Basic Clin Pharmacol Toxicol 100:115-20. 2007
    ..The results suggested that treatment with salvianolic acid A provides a potent protective effect against acute hepatic damage caused by CCl(4) in rats, which may mainly be related to its antioxidative effect...
  62. ncbi request reprint High throughput and rapid screening of marine protein hydrolysates enriched in peptides with angiotensin-I-converting enzyme inhibitory activity by capillary electrophoresis
    Hai Lun He
    State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China
    Bioresour Technol 98:3499-505. 2007
    ..However, the CE method was faster and, as a result, more economical. Therefore, CE had potential for rapid screening of marine protein hydrolysates enriched in ACE inhibitory peptides...
  63. pmc Parental smoking modifies the relation between genetic variation in tumor necrosis factor-alpha (TNF) and childhood asthma
    Hao Wu
    Laboratory of Respiratory Biology, Division of Intramural Research, National Institute of Environmental Health Sciences, National Institutes of Health DHHS, Research Triangle Park, NC 27709, USA
    Environ Health Perspect 115:616-22. 2007
    ..Parental smoking is a consistent risk factor for childhood asthma. Secondhand smoke and ozone both stimulate TNF production...
  64. pmc The Par-3 NTD adopts a PB1-like structure required for Par-3 oligomerization and membrane localization
    Wei Feng
    Department of Biochemistry, Molecular Neuroscience Center, The Hong Kong University of Science and Technology, Kowloon, Hong Kong
    EMBO J 26:2786-96. 2007
    ....
  65. ncbi request reprint Molecular basis of Bcl-xL's target recognition versatility revealed by the structure of Bcl-xL in complex with the BH3 domain of Beclin-1
    Wei Feng
    Department of Biochemistry, Molecular Neuroscience Center, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, P R China
    J Mol Biol 372:223-35. 2007
    ..Systematic analysis of all known Bcl-xL/BH3 domain complexes helped us to identify the molecular basis underlying the capacity of Bcl-xL to recognize diverse target sequences...
  66. pmc Clustering and synaptic targeting of PICK1 requires direct interaction between the PDZ domain and lipid membranes
    Lifeng Pan
    Department of Biochemistry, Molecular Neuroscience Center, Hong Kong University of Science and Technology, Kowloon, Hong Kong, PR China
    EMBO J 26:4576-87. 2007
    ..Taken together, our findings not only uncovered the novel lipid membrane-binding property of the PICK1 PDZ domain, but also provided direct evidence supporting the functional relevance of the PDZ-lipid interaction...
  67. ncbi request reprint [Role of cyclin kinase inhibitor p27 in inhibition of emodin on mesangial cell proliferation induced by tumor necrosis factor-alpha]
    Xiao Bin Mei
    Department of Nephrology, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China
    Zhong Xi Yi Jie He Xue Bao 2:120-2. 2004
    ..To investigate the role of p27 in the inhibition of emodin on the mesangial cell (MC) proliferation induced by tumor necrosis factor-alpha(TNF-alpha)...
  68. ncbi request reprint Par-3-mediated junctional localization of the lipid phosphatase PTEN is required for cell polarity establishment
    Wei Feng
    Department of Biochemistry, Molecular Neuroscience Center, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    J Biol Chem 283:23440-9. 2008
    ..These results, together with earlier data, firmly establish that Par-3 functions as a scaffold in integrating phosphoinositide signaling events during cellular polarization...
  69. pmc Role of MicroRNA miR-27a and miR-451 in the regulation of MDR1/P-glycoprotein expression in human cancer cells
    Hua Zhu
    Department of Pharmacology, The Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, New Brunswick, NJ 08903, United States
    Biochem Pharmacol 76:582-8. 2008
    ....
  70. ncbi request reprint The mechanism of p21-activated kinase 2 autoactivation
    Hao Wu
    National Laboratory of Biomacromolecules, Center for Molecular Biology, Institute of Biophysics, Academia Sinica, Beijing 100101, China
    J Biol Chem 278:41768-78. 2003
    ....
  71. ncbi request reprint Overexpression of extracellular matrix metalloproteinase inducer in multidrug resistant cancer cells
    Jin Ming Yang
    Department of Pharmacology, The Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, New Brunswick, NJ 08901, USA
    Mol Cancer Res 1:420-7. 2003
    ..Our results suggest that during the development of MDR, the expression of EMMPRIN is responsible for the increased activity of MMP in MDR cell lines...
  72. ncbi request reprint [Methods in the treatment of rheumatoid arthritis with Chinese herbal drugs: experimental study on collagen-induced arthritis]
    Ai Ping Lu
    Institute of Basic Theory, China Acaemy Of Tradtional Chinese Medicine
    Zhongguo Zhong Yao Za Zhi 29:505-7, 607. 2004

Research Grants34

  1. Structural and functional studies of the IkappaB kinase (IKK) complex
    Hao Wu; Fiscal Year: 2010
    ..In addition, the proposed studies will provide a structural basis for discovery and optimization of IKK inhibitors in the treatment of inflammatory diseases and cancer. ..
  2. Structural Studies of TNF Receptor Associated Factors (TRAFs)
    Hao Wu; Fiscal Year: 2007
    ..Role of TRAF6 deubiquitination in terminating TAK1 activation: specificity of the deubiquitinase A20 for TRAF6 Aim 3. Role of dimerization in XIAP and TAB1 mediated TAK1 activation ..
  3. Structural and Functional Studies of the Caspase Activating Complex PIDDosome
    Hao Wu; Fiscal Year: 2010
    ..The current application seeks to address a critical question in apoptosis regulation and has a strong implication in understanding and treatment of apoptosis-related human diseases. ..
  4. STRUCTURAL STUDIES OF TNF RECEPTOR ASSOCIATED FACTORS
    Hao Wu; Fiscal Year: 1999
    ..Therefore, the proposed study should have therapeutic implications by providing the possibility for structure-based approaches. ..
  5. Structural Studies of Nuclear Effectors of Apoptosis
    Hao Wu; Fiscal Year: 2006
    ..In this application, we propose to use a combination of X-ray crystallography, biochemistry and cell biology to elucidate the molecular basis of AIF and DFF function. ..
  6. Structural and functional studies of DISC assembly and FLIP inhibition
    Hao Wu; Fiscal Year: 2007
    ....
  7. Structural and functional studies of DISC assembly and FLIP inhibition
    Hao Wu; Fiscal Year: 2009
    ....
  8. Structural Studies of TNF Receptor Associated Factors (TRAFs)
    Hao Wu; Fiscal Year: 2009
    ..Role of TRAF6 deubiquitination in terminating TAK1 activation: specificity of the deubiquitinase A20 for TRAF6 Aim 3. Role of dimerization in XIAP and TAB1 mediated TAK1 activation ..
  9. Structural and Functional Studies of the Caspase Activating Complex PIDDosome
    Hao Wu; Fiscal Year: 2009
    ..The current application seeks to address a critical question in apoptosis regulation and has a strong implication in understanding and treatment of apoptosis-related human diseases. ..
  10. Structural and functional studies of the IkappaB kinase (IKK) complex
    Hao Wu; Fiscal Year: 2009
    ..In addition, the proposed studies will provide a structural basis for discovery and optimization of IKK inhibitors in the treatment of inflammatory diseases and cancer. ..
  11. Structural and functional studies of DISC assembly and FLIP inhibition
    Hao Wu; Fiscal Year: 2010
    ....
  12. Structural Studies of TNF Receptor Associated Factors (TRAFs)
    Hao Wu; Fiscal Year: 2010
    ..Role of TRAF6 deubiquitination in terminating TAK1 activation: specificity of the deubiquitinase A20 for TRAF6 Aim 3. Role of dimerization in XIAP and TAB1 mediated TAK1 activation ..
  13. Structural Studies of Protein Caspase Inhibitors
    Hao Wu; Fiscal Year: 2006
    ..In this proposal, we intend to determine crystal structures of several such complexes and to complement these structural studies with biochemical and mutational experiments. ..
  14. Structural Studies of Nuclear Effectors of Apoptosis
    Hao Wu; Fiscal Year: 2005
    ..In this application, we propose to use a combination of X-ray crystallography, biochemistry and cell biology to elucidate the molecular basis of AIF and DFF function. ..
  15. STRUCTURAL STUDIES OF DEATH RECEPTOR SIGNALING
    Hao Wu; Fiscal Year: 2000
    ..The proposed research should shed light on the structural basis for the transduction of signals from receptors to downstream effectors by these proteins. ..
  16. STRUCTURAL STUDIES OF TNF RECEPTOR ASSOCIATED FACTORS
    Hao Wu; Fiscal Year: 2000
    ..Therefore, the proposed study should have therapeutic implications by providing the possibility for structure-based approaches. ..
  17. STRUCTURAL STUDIES OF TNF RECEPTOR ASSOCIATED FACTORS
    Hao Wu; Fiscal Year: 2001
    ..Therefore, the proposed study should have therapeutic implications by providing the possibility for structure-based approaches. ..
  18. STRUCTURAL STUDIES OF DEATH RECEPTOR SIGNALING
    Hao Wu; Fiscal Year: 2001
    ..The proposed research should shed light on the structural basis for the transduction of signals from receptors to downstream effectors by these proteins. ..
  19. Structural Studies of Protein Caspase Inhibitors
    Hao Wu; Fiscal Year: 2002
    ..In this proposal, we intend to determine crystal structures of several such complexes and to complement these structural studies with biochemical and mutational experiments. ..
  20. STRUCTURAL STUDIES OF TNF RECEPTOR ASSOCIATED FACTORS
    Hao Wu; Fiscal Year: 2002
    ..Therefore, the proposed study should have therapeutic implications by providing the possibility for structure-based approaches. ..
  21. STRUCTURAL STUDIES OF DEATH RECEPTOR SIGNALING
    Hao Wu; Fiscal Year: 2002
    ..The proposed research should shed light on the structural basis for the transduction of signals from receptors to downstream effectors by these proteins. ..
  22. Structural Studies of Nuclear Effectors of Apoptosis
    Hao Wu; Fiscal Year: 2003
    ..In this application, we propose to use a combination of X-ray crystallography, biochemistry and cell biology to elucidate the molecular basis of AIF and DFF function. ..
  23. Structural Studies of Protein Caspase Inhibitors
    Hao Wu; Fiscal Year: 2003
    ..In this proposal, we intend to determine crystal structures of several such complexes and to complement these structural studies with biochemical and mutational experiments. ..
  24. STRUCTURAL STUDIES OF TNF RECEPTOR ASSOCIATED FACTORS
    Hao Wu; Fiscal Year: 2003
    ..Therefore, the proposed study should have therapeutic implications by providing the possibility for structure-based approaches. ..
  25. STRUCTURAL STUDIES OF DEATH RECEPTOR SIGNALING
    Hao Wu; Fiscal Year: 2003
    ..The proposed research should shed light on the structural basis for the transduction of signals from receptors to downstream effectors by these proteins. ..
  26. Structural Studies of Protein Caspase Inhibitors
    Hao Wu; Fiscal Year: 2004
    ..In this proposal, we intend to determine crystal structures of several such complexes and to complement these structural studies with biochemical and mutational experiments. ..
  27. Structural Studies of Nuclear Effectors of Apoptosis
    Hao Wu; Fiscal Year: 2004
    ..In this application, we propose to use a combination of X-ray crystallography, biochemistry and cell biology to elucidate the molecular basis of AIF and DFF function. ..
  28. STRUCTURAL STUDIES OF DEATH RECEPTOR SIGNALING
    Hao Wu; Fiscal Year: 2004
    ..The proposed research should shed light on the structural basis for the transduction of signals from receptors to downstream effectors by these proteins. ..
  29. Structural Studies of Protein Caspase Inhibitors
    Hao Wu; Fiscal Year: 2005
    ..In this proposal, we intend to determine crystal structures of several such complexes and to complement these structural studies with biochemical and mutational experiments. ..
  30. Molecular elucidation of the CBM complex in NF-kappaB activation by antigen recep
    Hao Wu; Fiscal Year: 2010
    ..These translocations likely activate NF-kB through MALT1 and Bcl10 overexpression. Collectively, these studies suggest that the CBM complex is an attractive therapeutic target neoplastic disorders of the lymphocytes. ..