Research Topics
Genomes and GenesSpecies | MARIANA WOLFNERSummaryAffiliation: Cornell University Country: USA Publications
Research Grants
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Detail Information
Publications
"S.P.E.R.M." (seminal proteins (are) essential reproductive modulators): the view from DrosophilaM F Wolfner
Department of Molecular Biology and Genetics, Cornell University, Ithaca NY 14853, USA
Soc Reprod Fertil Suppl 65:183-99. 2007..Results on additional roles of Acp-sperm interaction in Drosophila will be reviewed...
YA is needed for proper nuclear organization to transition between meiosis and mitosis in DrosophilaKatharine L Sackton
Dept of Molecular Biology and Genetics, Cornell University, Ithaca NY 14853, USA
BMC Dev Biol 9:43. 2009..After egg activation, YA enters nuclei and interacts with chromatin and the nuclear lamina. This study was designed to define more precisely the events prior to the first cleavage division that are dependent upon YA...- Tokens of love: functions and regulation of Drosophila male accessory gland productsM F Wolfner
Section of Genetics and Development, Cornell University, Ithaca, NY 14853 2703, USA
Insect Biochem Mol Biol 27:179-92. 1997.... - New genes for male accessory gland proteins in Drosophila melanogasterM F Wolfner
Section of Genetics and Development, Cornell University, Ithaca, NY 14853, USA
Insect Biochem Mol Biol 27:825-34. 1997.... - The nuclear envelope: emerging roles in development and diseaseM F Wolfner
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853 2703, USA
Cell Mol Life Sci 58:1737-40. 2001.... - The gifts that keep on giving: physiological functions and evolutionary dynamics of male seminal proteins in DrosophilaM F Wolfner
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853 2703, USA
Heredity (Edinb) 88:85-93. 2002..Supporting this hypothesis, an unusually high fraction of Acps show signs consistent with their being targets of positive Darwinian selection... - Sex determination: sex on the brain?Mariana F Wolfner
Dept of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853 2703, USA
Curr Biol 13:R101-3. 2003..The way these genes are regulated suggests new complexities in the sex determination pathway... - Battle and ballet: molecular interactions between the sexes in DrosophilaMariana F Wolfner
Department of Molecular Biology and Genetics, 423 Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
J Hered 100:399-410. 2009..Over evolutionary time this disconnect can, in theory, precipitate a "battle" between the sexes, potentially leading to the rapid sequence changes that have been observed for some seminal proteins across species... - Precious essences: female secretions promote sperm storage in DrosophilaMariana F Wolfner
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, United States of America
PLoS Biol 9:e1001191. 2011..Identification of these molecules also has potential practical implications for strategies to regulate the reproduction of insects of medical or agricultural importance... - Evidence for structural constraint on ovulin, a rapidly evolving Drosophila melanogaster seminal proteinAlex Wong
Department of Molecular Biology and Genetics, Biotechnology Building Room 423, Cornell University, Ithaca, NY 14853, USA
Proc Natl Acad Sci U S A 103:18644-9. 2006..We therefore suggest that domains involved in ovulin's self-interaction form a conserved structural backbone for the protein, resulting in greater evolutionary flexibility at other sites... - Sustained post-mating response in Drosophila melanogaster requires multiple seminal fluid proteinsK Ravi Ram
Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, New York, USA
PLoS Genet 3:e238. 2007..Our results also show that members of conserved protein classes found in seminal plasma from insects to mammals are essential for important reproductive processes... - Comparative structural modeling and inference of conserved protein classes in Drosophila seminal fluidJacob L Mueller
Department of Molecular Biology and Genetics, Biotechnology Building, Cornell Theory Center, Cornell University, Ithaca, NY 14853, USA
Proc Natl Acad Sci U S A 101:13542-7. 2004..This is particularly striking in the face of the rapid protein sequence evolution that characterizes many reproductive proteins, including Drosophila and mammalian seminal proteins... - The Drosophila nuclear lamina protein YA binds to DNA and histone H2B with four domainsJing Yu
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853 2703, USA
Mol Biol Cell 13:558-69. 2002..It also binds to histone H2B. YA's binding to DNA and histone H2B is mediated by four domains distributed along the length of the YA molecule. A model for YA function at the end of Drosophila female meiosis is proposed... - Evolution of genes and genomes on the Drosophila phylogenyAndrew G Clark
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
Nature 450:203-18. 2007..These may prove to underlie differences in the ecology and behaviour of these diverse species... - Seminal proteins but not sperm induce morphological changes in the Drosophila melanogaster female reproductive tract during sperm storageErika M Adams
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
J Insect Physiol 53:319-31. 2007..Our results suggest that timely morphological changes in the female reproductive tract, possibly muscular in nature, may be needed for successful sperm storage, and that Acps from the male are needed in order for these changes to occur... - Evidence for positive selection on Drosophila melanogaster seminal fluid protease homologsAlex Wong
Department of Molecular Biology and Genetics, Cornell University, NY, USA
Mol Biol Evol 25:497-506. 2008..The second gene, CG9997, was previously shown to be essential for normal sperm usage, suggesting that sexual selection may underlie its history of adaptation... 
Insect seminal fluid proteins: identification and functionFrank W Avila
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
Annu Rev Entomol 56:21-40. 2011..Here, we review recent identification of insect SFPs and discuss the multiple roles these proteins play in the postmating processes of female insects...- A hydrophilic lamin-binding domain from the Drosophila YA protein can target proteins to the nuclear envelopeShobana S Mani
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853 2703, USA
J Cell Sci 116:2067-72. 2003..This hydrophilic YA domain might provide a useful molecular tool for targeting heterologous non-membrane-associated proteins to the nuclear envelope... - An early role for the Drosophila melanogaster male seminal protein Acp36DE in female sperm storageMargaret C Bloch Qazi
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
J Exp Biol 206:3521-8. 2003.... - Sexual behavior: a seminal peptide stimulates appetitesAlex Wong
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
Curr Biol 16:R256-7. 2006..These findings contribute significantly to our understanding of mating behaviors and resource allocation, and may provide insights useful for controlling the reproduction of insect pests... - A network of interactions among seminal proteins underlies the long-term postmating response in DrosophilaK Ravi Ram
Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
Proc Natl Acad Sci U S A 106:15384-9. 2009..The requirements for manifestation of the LTR in Drosophila establish the paradigm that multiple seminal proteins can exert their actions through a multistep, multicomponent network of interactions... - The Drosophila melanogaster seminal fluid protein Acp62F is a protease inhibitor that is toxic upon ectopic expressionOliver Lung
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
Genetics 160:211-24. 2002.... - The genetic basis for male x female interactions underlying variation in reproductive phenotypes of DrosophilaClement Y Chow
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853 2703, USA
Genetics 186:1355-65. 2010..The results of this study begin to elucidate the complex genetic architecture of reproductive and sperm competition phenotypes and have significant implications for the evolution of male and female characters... - A role for Acp29AB, a predicted seminal fluid lectin, in female sperm storage in Drosophila melanogasterAlex Wong
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 13902, USA
Genetics 180:921-31. 2008..Moreover, Acp29AB's effects on sperm storage and sperm competition may explain previously documented evidence for positive selection on the Acp29AB locus... - Acp36DE is required for uterine conformational changes in mated Drosophila femalesFrank W Avila
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
Proc Natl Acad Sci U S A 106:15796-800. 2009..Rather, consistent with their role in later steps of sperm storage, we show here that their presence can affect the outcome of sperm competition situations... - Immortal coils: conserved dimerization motifs of the Drosophila ovulation prohormone ovulinAlex Wong
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
Insect Biochem Mol Biol 40:303-10. 2010..Consistent with this view, in comparisons of 8510 proteins across 6 species of Drosophila we find that rates of amino acid divergence are higher for proteins with coiled-coil protein-interaction domains than for non-coiled-coil proteins... - Post-mating gene expression profiles of female Drosophila melanogaster in response to time and to four male accessory gland proteinsLisa A McGraw
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
Genetics 179:1395-408. 2008..In contrast, Acp29AB and Acp62F modulated a large number of transcriptional changes shortly after mating... - Interaction of the essential Drosophila nuclear protein YA with P0/AP3 in the cytoplasm and in vitro: implications for developmental regulation of YA's subcellular locationJing Yu
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14850 2703, USA
Dev Biol 244:429-41. 2002..Hence, some YA dissociates from the complex and enters nuclei. Consistent with this model, decreasing P0 levels partially suppress a hypomorphic Ya mutant allele... - Two cleavage products of the Drosophila accessory gland protein ovulin can independently induce ovulationYael Heifetz
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
Proc Natl Acad Sci U S A 102:743-8. 2005..Thus, ovulin's cleavage in mated females is not destructive and instead may liberate additional functional products with potential to modulate ovulation independently... - Larval rearing environment affects several post-copulatory traits in Drosophila melanogasterLisa A McGraw
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
Biol Lett 3:607-10. 2007.... - Identity and transfer of male reproductive gland proteins of the dengue vector mosquito, Aedes aegypti: potential tools for control of female feeding and reproductionLaura K Sirot
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
Insect Biochem Mol Biol 38:176-89. 2008..melanogaster. Our results identify candidate male-derived molecules that may have an important influence on behavior, survival, and reproduction of female mosquitoes... - The developments between gametogenesis and fertilization: ovulation and female sperm storage in Drosophila melanogasterMargaret C Bloch Qazi
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
Dev Biol 256:195-211. 2003..melanogaster, both processes are triggered by male factors during and after mating, including sperm and seminal fluid proteins. Therefore, an interplay of male and female factors coordinates the gametes for fertilization... - An ectopic expression screen reveals the protective and toxic effects of Drosophila seminal fluid proteinsJacob L Mueller
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
Genetics 175:777-83. 2007..We also tested ectopic expression females for other Acp-induced effects, but found no additional Acps that affected egg laying or receptivity upon ectopic expression... - Genes regulated by mating, sperm, or seminal proteins in mated female Drosophila melanogasterLisa A McGraw
Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
Curr Biol 14:1509-14. 2004..The mating-dependent genes that we have identified contribute to many biological processes including metabolism, immune defense, and protein modification... - Predicted seminal astacin-like protease is required for processing of reproductive proteins in Drosophila melanogasterKristipati Ravi Ram
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
Proc Natl Acad Sci U S A 103:18674-9. 2006.... - Strain-dependent differences in several reproductive traits are not accompanied by early postmating transcriptome changes in female Drosophila melanogasterLisa A McGraw
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
Genetics 181:1273-80. 2009..melanogaster females are not caused by large modifications of transcript levels. Instead, early postmating phenotypes result from preexisting receptors or pathways that are already in place upon sexual maturity... - The Drosophila calcipressin sarah is required for several aspects of egg activationVanessa L Horner
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
Curr Biol 16:1441-6. 2006..We also find the conversion of the sperm nucleus into a functional male pronucleus is compromised in sarah mutant eggs, indicating that the Drosophila egg's competence to support male pronuclear maturation is acquired during activation... - Wispy, the Drosophila homolog of GLD-2, is required during oogenesis and egg activationJun Cui
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
Genetics 178:2017-29. 2008..Finally, we find that WISP function is also needed during oogenesis to regulate the poly(A) tail length of dmos during oocyte maturation and to maintain a high level of active (phospho-) mitogen-activated protein kinases (MAPKs)... - Molecular social interactions: Drosophila melanogaster seminal fluid proteins as a case studyLaura K Sirot
Department of Molecular Biology and Genetics, 421 Biotechnology Building, Cornell University, Ithaca, New York 14853, USA
Adv Genet 68:23-56. 2009..We conclude with a discussion of the ways in which Sfps can both shape and be shaped by (in an evolutionary sense) the molecular social interactions in which they are involved... - Mating, seminal fluid components, and sperm cause changes in vesicle release in the Drosophila female reproductive tractYael Heifetz
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
Proc Natl Acad Sci U S A 101:6261-6. 2004..This modulation could create an environment conducive to increased reproductive capacity... - Transitioning from egg to embryo: triggers and mechanisms of egg activationVanessa L Horner
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853 2703, USA
Dev Dyn 237:527-44. 2008..We review signals that trigger activation and how they link to subsequent molecular events of egg activation... - Mechanical stimulation by osmotic and hydrostatic pressure activates Drosophila oocytes in vitro in a calcium-dependent mannerVanessa L Horner
Department of Molecular Biology and Genetics, 423 Biotechnology Building, Cornell University, Ithaca, NY 14853 2703, USA
Dev Biol 316:100-9. 2008..This will allow exploitation of Drosophila genetics to dissect molecular pathways involving Ca(2+) and the activation of development... - Modulation of MAPK activities during egg activation in DrosophilaKatharine L Sackton
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
Fly (Austin) 1:222-7. 2007..We present a model in which the decrease in MAPK activity is an intermediate step in the pathway leading from the calcium signal that initiates egg activation to the downstream events of activation...
Research Grants
- Molecular Genetics of Pronuclear Functions in DrosphilaMARIANA WOLFNER; Fiscal Year: 2005..Such modulation could mediate the rapid, concerted changes in egg physiology that occur at that time. ..
- Molecular genetics of egg activation and pronuclear function in DrosophilaMARIANA WOLFNER; Fiscal Year: 2007..Thus, we exploit the powerful genetics of Drosophila to elucidate efficiently the molecules and gene pathways needed to start development in humans. ..
- ACTIONS OF SEMINAL PROTEINS IN MATED DROSOPHILA FEMALESMARIANA WOLFNER; Fiscal Year: 2007..In addition to their relevance to reproductive mechanisms in a range of organisms including mammals, we anticipate that our findings will have practical applications in the control of insect vectors of human disease. ..
- ACTIONS OF SEMINAL PROTEINS IN MATED DROSOPHILA FEMALESMARIANA FEDERICA WOLFNER; Fiscal Year: 2010..Because of analogous actions of seminal proteins across animals, our results will inform understanding of certain infertilities in humans, and methods to control the reproduction of insect vectors of human disease. ..
- Molecular genetics of egg activation and pronuclear function in DrosophilaMARIANA FEDERICA WOLFNER; Fiscal Year: 2010..Thus, we exploit the powerful genetics of Drosophila to elucidate efficiently the molecules and gene pathways needed to start development in humans. ..
- ACTIONS OF SEMINAL PROTEINS IN MATED DROSOPHILA FEMALESMARIANA WOLFNER; Fiscal Year: 2003..Analyzing these mutants, and ectopic expression of Acps, will identify new Acps that regulate ovulation, sperm storage or proteolysis. ..
- MOLECULAR GENETICS OF PRONUCLEAR FUNCTIONS IN DROSOPHILAMARIANA WOLFNER; Fiscal Year: 1993..Simultaneously, they will permit the first genetic dissection of a non-lamin nuclear envelope component, and its developmental function, in an animal...
- MOLECULAR GENETICS OF PRONUCLEAR FUNCTIONS IN DROSOPHILAMARIANA WOLFNER; Fiscal Year: 2001..Finally, we will elucidate the developmental controls that permit YA to enter nuclei upon egg activation, and the signals that target YA to the nuclear lamina. ..
- ACTIONS OF SEMINAL PROTEINS IN MATED DROSOPHILA FEMALESMARIANA WOLFNER; Fiscal Year: 2009..In addition to their relevance to reproductive mechanisms in a range of organisms including mammals, we anticipate that our findings will have practical applications in the control of insect vectors of human disease. ..