Research Topics
Genomes and GenesSpecies | Elad TakoSummaryAffiliation: USDA-ARS Location: Ithaca, NY, USA Summary: My research overall goal is to alleviate dietary micronutrient deficiencies in at risk populations. Additionally, my research focuses on better understanding the cellular pathways that participate and/or control iron and zinc intestinal absorption. Specialties My research approach utilizes both cellular and animal models to assess mineral bioavailability (including iron and zinc) in standard and biofortified food crops (including maize, beans and lentil). Publications: Using the domestic chicken (Gallus gallus) as an in vivo model Journal Of Poultry Science|March 2010 Authors: Elad Tako, et al Abstract Iron fortification of foods and biofortification of staple food crops are strategies that can help to alleviate Fe deficiency. The broiler chicken may be a useful model for initial in vivo screening of Fe bioavailability in foods due to its growth rate, anatomy, size, and low cost. In this study, we assess the broiler as a model for hemoglobin (Hb) maintenance studies and present a...more White beans provide more bioavailable iron than red beans: studies in poultry (Gallus gallus) and an in vitro digestion/Caco-2 model Int J Vitam Nutr Res.|December 2010 Authors: Elad Tako, et al Abstract Iron-biofortification of crops is a strategy that alleviates iron deficiency. The common bean (Phaseolus vulgaris L.) is an attractive candidate for biofortification. However, beans are high in polyphenols that may inhibit iron absorption. In vitro studies have shown that iron bioavailability from white beans is higher than that from colored beans. In this study, our objective was to...more Iron Status of the Late Term Broiler (Gallus gallus) Embryo and Hatchling International Journal of Poultry Science|January 2011 Authors: Elad Tako, Glahn RP Abstract Our objective was to investigate and elucidate the iron status of the late term broiler (Gallus gallus) embryo and young chick. This would be vital for better understanding of the transition period that the hatchling experience immediately after hatch. For that, blood, liver and small intestinal samples from broiler embryos were taken on embryonic days 12 (12E), 14E, 17E, 19E, day of...more Biofortified black beans in a maize and bean diet provide more bioavailable iron to piglets than standard black beans. Journal Of Nutrition|February 2009 Authors: Elad Tako, et al Abstract Our objective was to compare the capacities of biofortified and standard black beans (Phaseolus vulgaris L.) to deliver iron (Fe) for hemoglobin (Hb) synthesis. Two lines of black beans, one standard and the other biofortified (high) in Fe (71 and 106 microg Fe/g, respectively), were used. Maize-based diets containing the beans were formulated to meet the nutrient requirements for swine...more Dietary inulin affects the expression of intestinal enterocyte iron transporters, receptors and storage protein and alters the microbiota in the pig intestine British Journal Of Nutrition|March 2008 Authors: Elad Tako, et al Abstract Inulin, a linear beta fructan, is present in a variety of plants including chicory root and wheat. It exhibits prebiotic properties and has been shown to enhance mineral absorption and increase beneficial bacteria in the colon. The aim of the present study was to assess the effect of dietary inulin on the gene expression of selected intestinal Fe transporters and binding proteins....more Iron and zinc bioavailabilities to pigs from red and white beans (Phaseolus vulgaris L.) are similar. Journal Of Agricultural and Food Chemistry|April 2009 Authors: Elad Tako, et al Abstract Common beans contain relatively high concentrations of iron (Fe) and zinc (Zn) but are also high in polyphenols and phytates, factors that may inhibit Fe and Zn absorption. In vitro (Caco-2 cells) and in vivo (pigs) models were used to compare Fe and Zn bioavailabilities between red and white beans, which differ in polyphenol content. Bean/maize diets containing 37% of either white or...more Changes in chicken intestinal zinc exporter mRNA expression and small intestinal functionality following intra-amniotic zinc-methionine administration. J Nutr Biochem|June 2005 Authors: Elad Tako, et al Abstract A 303-bp cDNA of intestinal zinc exporter (ZnT1) was isolated from chicken jejunum by reverse transcriptase-polymerase chain reaction and sequenced, and showed 42% homology to Homo sapiens and Rattus novergicus intestinal ZnT1 genes. This specific probe was used to examine the effect of zinc-methionine (ZnMet) administration on the mRNA expression of ZnT1 and on small intestinal...more Biofortified red mottled beans (Phaseolus vulgaris L.) in a maize and bean diet provide more bioavailable iron than standard red mottled beans: Studies in poultry (Gallus gallus) and an in vitro digestion/Caco-2 model. Nutrition Journal|October 14, 2011 Authors: Elad Tako, et al Abstract Background: Our objective was to compare the capacities of biofortified and standard colored beans to deliver iron (Fe) for hemoglobin synthesis. Two isolines of large-seeded, red mottled Andean beans (Phaseolus vulgaris L.), one standard ("Low Fe") and the other biofortified ("High Fe") in Fe (49 and 71 μg Fe/g, respectively) were used. This commercial class of red mottled beans is the...more Publications
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Publications
White beans provide more bioavailable iron than red beans: studies in poultry (Gallus gallus) and an in vitro digestion/Caco-2 modelElad Tako
Department of Food Science, Cornell University, Ithaca, NY 14853, USA
Int J Vitam Nutr Res 80:416-29. 2010..We conclude that the in vivo results support the in vitro observations; i. e., white beans contain more bioavailable iron than red beans...- Iron and zinc bioavailabilities to pigs from red and white beans (Phaseolus vulgaris L.) are similarElad Tako
Department of Food Science and Animal Science, Cornell University, Ithaca, New York 15853, USA
J Agric Food Chem 57:3134-40. 2009..DMT1 gene expression did not differ between treatments. It was concluded that bean color does not affect Fe or Zn bioavailability in vivo and that beans are a good source of bioavailable Fe and Zn... 
Changes in chicken intestinal zinc exporter mRNA expression and small intestinal functionality following intra-amniotic zinc-methionine administrationElad Tako
Department of Animal Sciences, The Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P O Box 12, Rehovot 76100, Israel
J Nutr Biochem 16:339-46. 2005..These results suggest that ZnMet administration into prenatal intestine via injection into the amniotic fluid enhances intestinal development and improves its functionality...- Biofortified black beans in a maize and bean diet provide more bioavailable iron to piglets than standard black beansElad Tako
Department of Food Science, Cornell University, Ithaca, NY 14853, USA
J Nutr 139:305-9. 2009..4 +/- 0.4 micromol)] (P = 0.034). We conclude that the biofortified beans are a promising vehicle for increasing intakes of bioavailable Fe in human populations that consume beans as a dietary staple... - Dietary inulin affects the expression of intestinal enterocyte iron transporters, receptors and storage protein and alters the microbiota in the pig intestineE Tako
Department of Food Science, Cornell University, Itacha, NY 14853, USA
Br J Nutr 99:472-80. 2008..05) compared with the control group. These results indicate that dietary inulin might trigger an up regulation of genes encoding for Fe transporters in the enterocyte. The specific mechanism for this effect remains to be elucidated... 
High bioavailablilty iron maize (Zea mays L.) developed through molecular breeding provides more absorbable iron in vitro (Caco-2 model) and in vivo (Gallus gallus)Elad Tako
USDA ARS Robert W, Holley Center for Agriculture and Health, 538 Tower Road, Cornell University, Ithaca, NY 14853, USA
Nutr J 12:3. 2013..Also, to compare the capacities of our two maize hybrid varieties to deliver Fe for hemoglobin (Hb) synthesis and to improve the Fe status of Fe deficient broiler chickens...- Evaluation of metallothionein formation as a proxy for zinc absorption in an in vitro digestion/Caco-2 cell culture modelZhiqiang Cheng
USDA ARS Robert W Holley Center for Agricultural and Health, Cornell University, Ithaca, NY 14853, USA
Food Funct 3:732-6. 2012..PA was observed to be a potent inhibitor of Zn uptake. Under the conditions of this in vitro model, Caco-2 cell monolayers are not useful for evaluating the Zn availability from foods... - Intra-amniotic administration and dietary inulin affect the iron status and intestinal functionality of iron-deficient broiler chickensE Tako
USDA ARS, Robert W Holley Center for Agriculture and Health, Cornell University, Ithaca, NY 14853, USA
Poult Sci 91:1361-70. 2012..05) in the inulin group versus controls. Results indicate that intra-amniotic administration and dietary inulin improved the iron status of iron-deficient broilers... - Biofortified red mottled beans (Phaseolus vulgaris L.) in a maize and bean diet provide more bioavailable iron than standard red mottled beans: studies in poultry (Gallus gallus) and an in vitro digestion/Caco-2 modelElad Tako
USDA ARS, Robert W, Holley Center for Agriculture and Health, Cornell University, Ithaca, NY 14853, USA
Nutr J 10:113. 2011..Therefore it is important to know the affect of biofortification of these beans on diets that simulate human feeding studies... - Using the domestic chicken (Gallus gallus) as an in vivo model for iron bioavailabilityE Tako
Department of Science, Cornell University, Ithaca, NY 14853, USA
Poult Sci 89:514-21. 2010..Such a model should be most useful as an intermediate test of in vivo Fe bioavailability observations in preparation for subsequent human studies... - Mucin gene expression and mucin content in the chicken intestinal goblet cells are affected by in ovo feeding of carbohydratesA Smirnov
The Faculty of Agricultural, Food and Environmental Quality Sciences, Department of Animal Sciences, Hebrew University of Jerusalem, PO Box 12, Rehovot, 76100, Israel
Poult Sci 85:669-73. 2006..The results showed that providing the carbohydrates as an energy source to the late-term embryo had a trophic effect on the small intestine and enhanced goblet cell development... - In ovo feeding improves energy status of late-term chicken embryosZ Uni
Faculte of Agricultural, Food and Environmental Quality Sciences, Department of Animal Sciences, Hebrew University of Jerusalem, PO Box 12, Rehovot, 76100, Israel
Poult Sci 84:764-70. 2005.... - Effects of in ovo feeding of carbohydrates and beta-hydroxy-beta-methylbutyrate on the development of chicken intestineE Tako
The Faculty of Agricultural, Food and Environmental Quality Sciences, The Department of Animal Sciences, Hebrew University of Jerusalem, Rehovot, Israel
Poult Sci 83:2023-8. 2004..This advantage probably leads to higher BW in IO fed chicks... - Morphological, molecular, and functional changes in the chicken small intestine of the late-term embryoZ Uni
The Faculty of Agricultural, Food and Environmental Quality Sciences, Department of Animal Sciences, P O Box 12, Rehovot 76100, Israel
Poult Sci 82:1747-54. 2003..Thus, major changes in the expression and localization of the functional brush-border proteins prepare the framework for ingestion of carbohydrate- and protein-rich exogenous feed posthatch...