MARTHA STIPANUK

Summary

Affiliation: Cornell University
Country: USA

Publications

  1. ncbi request reprint Enzymes and metabolites of cysteine metabolism in nonhepatic tissues of rats show little response to changes in dietary protein or sulfur amino acid levels
    Martha H Stipanuk
    Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA
    J Nutr 132:3369-78. 2002
  2. ncbi request reprint Mammalian cysteine metabolism: new insights into regulation of cysteine metabolism
    Martha H Stipanuk
    Division of Nutritional Sciences, Cornell University, Ithaca, NY, USA
    J Nutr 136:1652S-1659S. 2006
  3. pmc Extrahepatic tissues compensate for loss of hepatic taurine synthesis in mice with liver-specific knockout of cysteine dioxygenase
    Iori Ueki
    Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA
    Am J Physiol Endocrinol Metab 302:E1292-9. 2012
  4. ncbi request reprint Leucine and protein synthesis: mTOR and beyond
    Martha H Stipanuk
    Division of Nutritional Sciences, 227 Savage Hall, Cornell University, Ithaca, NY 14853, USA
    Nutr Rev 65:122-9. 2007
  5. ncbi request reprint The keto acid of methionine is a safe and efficacious substitute for dietary L-methionine: the answer from chick bioassays
    Martha H Stipanuk
    Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA
    J Nutr 137:1844-5. 2007
  6. ncbi request reprint Sulfur amino acid metabolism: pathways for production and removal of homocysteine and cysteine
    Martha H Stipanuk
    Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA
    Annu Rev Nutr 24:539-77. 2004
  7. ncbi request reprint Role of the liver in regulation of body cysteine and taurine levels: a brief review
    Martha H Stipanuk
    227 Savage Hall, Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA
    Neurochem Res 29:105-10. 2004
  8. pmc Cysteine dioxygenase: a robust system for regulation of cellular cysteine levels
    M H Stipanuk
    Division of Nutritional Sciences, Cornell University, 227 Savage Hall, Ithaca, NY 14853, USA
    Amino Acids 37:55-63. 2009
  9. ncbi request reprint Evidence for expression of a single distinct form of mammalian cysteine dioxygenase
    M H Stipanuk
    Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853 6301, USA
    Amino Acids 26:99-106. 2004
  10. ncbi request reprint The ubiquitin-proteasome system is responsible for cysteine-responsive regulation of cysteine dioxygenase concentration in liver
    Martha H Stipanuk
    Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853 6301, USA
    Am J Physiol Endocrinol Metab 286:E439-48. 2004

Research Grants

  1. NUTRITIONAL REGULATION OF CYSTEINE DIOXYGENASE
    Martha H Stipanuk; Fiscal Year: 2010
  2. NUTRITIONAL REGULATION OF CYSTEINE DIOXYGENASE
    MARTHA STIPANUK; Fiscal Year: 2009
  3. Nutritional Regulation of y-Glutamylcysteine Synthetase
    MARTHA STIPANUK; Fiscal Year: 2007
  4. Nutritional Regulation of Cystein Dioxygenase
    MARTHA STIPANUK; Fiscal Year: 2007
  5. NUTRITIONAL REGULATION OF CYSTEINE DIOXYGENASE
    MARTHA STIPANUK; Fiscal Year: 2002
  6. NUTRITIONAL REGULATION OF CYSTEINE DIOXYGENASE
    MARTHA STIPANUK; Fiscal Year: 2009

Collaborators

  • John E Dominy
  • L Wang
  • W Wang
  • Quinton R Rogers
  • T P Begley
  • Chad R Simmons
  • Jeong In Lee
  • Iori Ueki
  • Lawrence L Hirschberger
  • Heather B Roman
  • L L Hirschberger
  • Y H Kwon
  • P Andrew Karplus
  • Relicardo M Coloso
  • Kwang Suk Ko
  • Quan Hao
  • Carrie L Cresenzi
  • D L Bella
  • Carolyn Junior
  • Jimmy Lam
  • Rachel Peters
  • Alessandro Valli
  • Krista Fieselmann
  • David J Schuller
  • Spencer L Granett
  • Kalyanaraman Krishnamoorthy
  • Angelos K Sikalidis
  • Lawrence Hirschberger
  • Joann Kang
  • Mari S Machi
  • Cristina L Torres
  • Qun Liu
  • Andrea J Fascetti
  • Qingqiu Huang
  • Monica Londono
  • S Daval
  • P J Stover
  • P J Bagley

Detail Information

Publications36

  1. ncbi request reprint Enzymes and metabolites of cysteine metabolism in nonhepatic tissues of rats show little response to changes in dietary protein or sulfur amino acid levels
    Martha H Stipanuk
    Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA
    J Nutr 132:3369-78. 2002
    ..Overall, the results confirm the importance of the liver in the maintenance of cysteine homeostasis...
  2. ncbi request reprint Mammalian cysteine metabolism: new insights into regulation of cysteine metabolism
    Martha H Stipanuk
    Division of Nutritional Sciences, Cornell University, Ithaca, NY, USA
    J Nutr 136:1652S-1659S. 2006
    ....
  3. pmc Extrahepatic tissues compensate for loss of hepatic taurine synthesis in mice with liver-specific knockout of cysteine dioxygenase
    Iori Ueki
    Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA
    Am J Physiol Endocrinol Metab 302:E1292-9. 2012
    ....
  4. ncbi request reprint Leucine and protein synthesis: mTOR and beyond
    Martha H Stipanuk
    Division of Nutritional Sciences, 227 Savage Hall, Cornell University, Ithaca, NY 14853, USA
    Nutr Rev 65:122-9. 2007
    ....
  5. ncbi request reprint The keto acid of methionine is a safe and efficacious substitute for dietary L-methionine: the answer from chick bioassays
    Martha H Stipanuk
    Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA
    J Nutr 137:1844-5. 2007
  6. ncbi request reprint Sulfur amino acid metabolism: pathways for production and removal of homocysteine and cysteine
    Martha H Stipanuk
    Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA
    Annu Rev Nutr 24:539-77. 2004
    ..Its concentration is regulated primarily by hepatic Cys dioxygenase; the level of Cys dioxygenase is upregulated in a Cys-responsive manner via a decrease in the rate of polyubiquitination and, hence, degradation by the 26S proteasome...
  7. ncbi request reprint Role of the liver in regulation of body cysteine and taurine levels: a brief review
    Martha H Stipanuk
    227 Savage Hall, Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA
    Neurochem Res 29:105-10. 2004
    ..The upregulation of cysteine dioxygenase in the presence of cysteine is a consequence of diminished ubiquitination of cysteine dioxygenase and a slower rate of degradation by the 26S proteasome...
  8. pmc Cysteine dioxygenase: a robust system for regulation of cellular cysteine levels
    M H Stipanuk
    Division of Nutritional Sciences, Cornell University, 227 Savage Hall, Ithaca, NY 14853, USA
    Amino Acids 37:55-63. 2009
    ....
  9. ncbi request reprint Evidence for expression of a single distinct form of mammalian cysteine dioxygenase
    M H Stipanuk
    Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853 6301, USA
    Amino Acids 26:99-106. 2004
    ..e., 23.5 kDa based on mass spectrometry) monomer and that this active form is probably derived by post-translational modification of the 23 kDa gene product...
  10. ncbi request reprint The ubiquitin-proteasome system is responsible for cysteine-responsive regulation of cysteine dioxygenase concentration in liver
    Martha H Stipanuk
    Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853 6301, USA
    Am J Physiol Endocrinol Metab 286:E439-48. 2004
    ..This regulation occurs in primary hepatocytes in a manner that corresponds with changes observed in intact animals...
  11. doi request reprint Macroautophagy and its role in nutrient homeostasis
    Martha H Stipanuk
    Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA
    Nutr Rev 67:677-89. 2009
    ..In addition, the recent placement of the Atg1*Atg13*Atg17 complex downstream of mTORC1 has provided at least one pathway by which changes in nutrient availability regulate autophagy...
  12. pmc Dealing with methionine/homocysteine sulfur: cysteine metabolism to taurine and inorganic sulfur
    Martha H Stipanuk
    Division of Nutritional Sciences, Cornell University, Ithaca, NY, 14853, USA
    J Inherit Metab Dis 34:17-32. 2011
    ..Cysteine dioxygenase is robustly regulated in response to cysteine availability and may function to provide a pathway to siphon cysteine to less toxic metabolites than those produced by cysteine desulfuration reactions...
  13. pmc Thiol dioxygenases: unique families of cupin proteins
    Martha H Stipanuk
    Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA
    Amino Acids 41:91-102. 2011
    ..In ADOs, the conserved glutamate residue in cupin motif 1 is replaced by either glycine or valine. Both ADOs and CDOs appear to represent unique clades within the cupin superfamily...
  14. ncbi request reprint Murine cysteine dioxygenase gene: structural organization, tissue-specific expression and promoter identification
    L L Hirschberger
    Division of Nutritional Sciences, Cornell University, Ithaca, New York, NY14853, USA
    Gene 277:153-61. 2001
    ..Western blot analyses indicated that CDO protein levels parallel mRNA levels. These results are consistent with the known function of CDO in whole-body cysteine homeostasis...
  15. ncbi request reprint Evaluation and modification of an assay procedure for cysteine dioxygenase activity: high-performance liquid chromatography method for measurement of cysteine sulfinate and demonstration of physiological relevance of cysteine dioxygenase activity in cyste
    P J Bagley
    Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA
    Anal Biochem 227:40-8. 1995
    ....
  16. ncbi request reprint Cysteine regulates expression of cysteine dioxygenase and gamma-glutamylcysteine synthetase in cultured rat hepatocytes
    Y H Kwon
    Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA
    Am J Physiol Endocrinol Metab 280:E804-15. 2001
    ..Taken together, these results suggest that cysteine itself is an important cellular signal for upregulation of CDO and downregulation of GCS...
  17. pmc Knockout of the murine cysteine dioxygenase gene results in severe impairment in ability to synthesize taurine and an increased catabolism of cysteine to hydrogen sulfide
    Iori Ueki
    Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA
    Am J Physiol Endocrinol Metab 301:E668-84. 2011
    ..Control of cysteine levels by regulation of CDO may be necessary to maintain low H(2)S/sulfane sulfur levels and facilitate the use of H(2)S as a signaling molecule...
  18. ncbi request reprint Cysteine metabolism in periportal and perivenous hepatocytes: perivenous cells have greater capacity for glutathione production and taurine synthesis but not for cysteine catabolism
    D L Bella
    Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853 6301, USA
    Amino Acids 23:453-8. 2002
    ..Cysteinesulfnate decarboxylase can be added to the list of enzymes whose activities are markedly enriched in perivenous cells...
  19. ncbi request reprint Cysteine is the metabolic signal responsible for dietary regulation of hepatic cysteine dioxygenase and glutamate cysteine ligase in intact rats
    Carrie L Cresenzi
    Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA
    J Nutr 133:2697-702. 2003
    ..These experiments are consistent with a role for cysteine, rather than a precursor or metabolite of cysteine, in the metabolic signaling responsible for diet-induced regulation of CDO and GCL...
  20. ncbi request reprint Regulation of cysteine dioxygenase and gamma-glutamylcysteine synthetase is associated with hepatic cysteine level
    Jeong In Lee
    Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA
    J Nutr Biochem 15:112-22. 2004
    ..These findings support a role of cysteine concentration as a mediator of its own metabolism, favoring catabolism when cysteine is high and glutathione synthesis when cysteine is low...
  21. pmc A putative Fe2+-bound persulfenate intermediate in cysteine dioxygenase
    Chad R Simmons
    Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA
    Biochemistry 47:11390-2. 2008
    ..A nonpolar environment of the distal oxygen would facilitate isomerization of the persulfenate to the sulfinate product...
  22. pmc Synthesis of amino acid cofactor in cysteine dioxygenase is regulated by substrate and represents a novel post-translational regulation of activity
    John E Dominy
    Department of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA
    J Biol Chem 283:12188-201. 2008
    ..Overall, the regulation of Cys-Tyr cofactor formation in CDO by ambient cysteine levels represents an unusual form of substrate-mediated feed-forward activation of enzyme activity with important physiological consequences...
  23. doi request reprint HepG2/C3A cells respond to cysteine deprivation by induction of the amino acid deprivation/integrated stress response pathway
    Jeong In Lee
    Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA
    Physiol Genomics 33:218-29. 2008
    ..A consequence of sulfur amino acid restriction appears to be the upregulation of the cellular capacity to cope with oxidative and chemical stresses via the integrated stress response...
  24. pmc Enzymes of the taurine biosynthetic pathway are expressed in rat mammary gland
    Iori Ueki
    Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA
    J Nutr 137:1887-94. 2007
    ....
  25. ncbi request reprint Discovery and characterization of a second mammalian thiol dioxygenase, cysteamine dioxygenase
    John E Dominy
    Department of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA
    J Biol Chem 282:25189-98. 2007
    ..Overall, these data suggest that ADO is responsible for endogenous cysteamine dioxygenase activity...
  26. pmc Differential regulation of glutamate-cysteine ligase subunit expression and increased holoenzyme formation in response to cysteine deprivation
    Jeong In Lee
    Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA
    Biochem J 393:181-90. 2006
    ..This is the first report demonstrating that the catalytic efficiency of rat GCL is increased by holoenzyme formation and the first demonstration of differential up-regulation of the GCL subunits in response to cysteine deprivation...
  27. ncbi request reprint Overexpression of cysteine dioxygenase reduces intracellular cysteine and glutathione pools in HepG2/C3A cells
    John E Dominy
    Department of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA
    Am J Physiol Endocrinol Metab 293:E62-9. 2007
    ..WT CDO also decreased the glutathione pool and potentiated the toxicity of CdCl(2). These results demonstrate that CDO is capable of altering intracellular cysteine levels as well as glutathione levels...
  28. ncbi request reprint In vivo regulation of cysteine dioxygenase via the ubiquitin-26S proteasome system
    John E Dominy
    Cornell University, Division of Nutritional Sciences, Ithaca, 14853 New York, USA
    Adv Exp Med Biol 583:37-47. 2006
  29. ncbi request reprint Cysteamine dioxygenase: evidence for the physiological conversion of cysteamine to hypotaurine in rat and mouse tissues
    Relicardo M Coloso
    Cornell University, Division of Nutritional Sciences, Ithaca, New York 148453, USA
    Adv Exp Med Biol 583:25-36. 2006
  30. ncbi request reprint Copper deficiency does not lead to taurine deficiency in rats
    Kwang Suk Ko
    Department of Molecular Biosciences, School of Veterinary Medicine, University of California Davis, CA 95616, USA
    J Nutr 136:2502-5. 2006
    ..1 vs. 99 +/- 5.3 nmol/g and liver CDO activity, 2.19 +/- 0.33 vs. 2.74 +/- 0.21 nmol x min(-1) x mg protein(-1). These findings support the conclusion that Cu deficiency does not affect body taurine status...
  31. pmc Regulation of cysteine dioxygenase degradation is mediated by intracellular cysteine levels and the ubiquitin-26 S proteasome system in the living rat
    John E Dominy
    Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA
    Biochem J 394:267-73. 2006
    ..The present study represents the first demonstration of regulated ubiquitination and degradation of a protein in a living mammal, inhibition of which had dramatic effects on cysteine catabolism...
  32. ncbi request reprint Expression, purification, and kinetic characterization of recombinant rat cysteine dioxygenase, a non-heme metalloenzyme necessary for regulation of cellular cysteine levels
    Chad R Simmons
    Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853 6301, USA
    Protein Expr Purif 47:74-81. 2006
    ..45 mM for L-cysteine. The expression and purification of CDO in a stable, highly active form has yielded significant insight into the kinetic properties of this unique thiol dioxygenase...
  33. pmc Preparation, crystallization and X-ray diffraction analysis to 1.5 A resolution of rat cysteine dioxygenase, a mononuclear iron enzyme responsible for cysteine thiol oxidation
    Chad R Simmons
    Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853 8001, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:1013-6. 2005
    ..55, c = 123.06 A, alpha = beta = gamma = 90 degrees. CDO shows little homology to any other proteins; therefore, the structure of the enzyme will be determined by ab initio phasing using a selenomethionyl derivative...
  34. ncbi request reprint New roles for cysteine and transsulfuration enzymes: production of H2S, a neuromodulator and smooth muscle relaxant
    John E Dominy
    Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA
    Nutr Rev 62:348-53. 2004
    ..In smooth muscle cells, H2S produced by cystathionine gamma-lyase enhances the outward flux of potassium by opening potassium channels, leading to hyperpolarization of membrane potential and smooth muscle relaxation...
  35. ncbi request reprint Crystal structure of mammalian cysteine dioxygenase. A novel mononuclear iron center for cysteine thiol oxidation
    Chad R Simmons
    Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853 8001, USA
    J Biol Chem 281:18723-33. 2006
    ....
  36. pmc Identification and characterization of bacterial cysteine dioxygenases: a new route of cysteine degradation for eubacteria
    John E Dominy
    Division of Nutritional Sciences, 227 Savage Hall, Cornell University, Ithaca, NY 14853, USA
    J Bacteriol 188:5561-9. 2006
    ..Suggestions are made for how this novel pathway of cysteine metabolism may play a role in the life cycle of the eubacteria that have it...

Research Grants15

  1. NUTRITIONAL REGULATION OF CYSTEINE DIOXYGENASE
    Martha H Stipanuk; Fiscal Year: 2010
    ....
  2. NUTRITIONAL REGULATION OF CYSTEINE DIOXYGENASE
    MARTHA STIPANUK; Fiscal Year: 2009
    ....
  3. Nutritional Regulation of y-Glutamylcysteine Synthetase
    MARTHA STIPANUK; Fiscal Year: 2007
    ..Capacity for GSH synthesis, availability of the precursor amino acid cysteine, and steady-state GSH levels all contribute to the body's ability to defend itself against oxidative or chemical stressors. ..
  4. Nutritional Regulation of Cystein Dioxygenase
    MARTHA STIPANUK; Fiscal Year: 2007
    ..d.) To evaluate the physiological significance of CDO in the regulation of cellular cysteine (and gtutathione) level. ..
  5. NUTRITIONAL REGULATION OF CYSTEINE DIOXYGENASE
    MARTHA STIPANUK; Fiscal Year: 2002
    ..abstract_text> ..
  6. NUTRITIONAL REGULATION OF CYSTEINE DIOXYGENASE
    MARTHA STIPANUK; Fiscal Year: 2009
    ....