John P Moore


Affiliation: Cornell University
Country: USA


  1. Ringe R, Pugach P, Cottrell C, LaBranche C, Seabright G, Ketas T, et al. Closing and opening holes in the glycan shield of HIV-1 envelope glycoprotein SOSIP trimers can redirect the neutralizing antibody response to the newly unmasked epitopes. J Virol. 2018;: pubmed publisher
    ..Thus, in principle, the antibody response might be diverted from one site to a more cross-reactive one, which would help in the induction of broadly neutralizing antibodies by HIV-1 vaccines based on envelope glycoproteins. ..
  2. Sanders R, van Gils M, Derking R, Sok D, Ketas T, Burger J, et al. HIV-1 VACCINES. HIV-1 neutralizing antibodies induced by native-like envelope trimers. Science. 2015;349:aac4223 pubmed publisher
    ..A second trimer, B41 SOSIP.664, also induced a strong autologous tier 2 NAb response in rabbits. Thus, native-like trimers represent a promising starting point for the development of HIV-1 vaccines aimed at inducing bNAbs. ..
  3. Sullivan J, Sulli C, Nilo A, Yasmeen A, Ozorowski G, Sanders R, et al. High-Throughput Protein Engineering Improves the Antigenicity and Stability of Soluble HIV-1 Envelope Glycoprotein SOSIP Trimers. J Virol. 2017;91: pubmed publisher
    ..The strategies described here may be useful for identifying a wider range of antigenically and structurally improved soluble trimers based on multiple genotypes for use in programs intended to create a broadly protective HIV-1 vaccine. ..
  4. Struwe W, Chertova E, Allen J, Seabright G, Watanabe Y, Harvey D, et al. Site-Specific Glycosylation of Virion-Derived HIV-1 Env Is Mimicked by a Soluble Trimeric Immunogen. Cell Rep. 2018;24:1958-1966.e5 pubmed publisher
    ..Knowledge of native versus recombinant Env glycosylation will guide vaccine design and manufacturing programs. ..
  5. Sliepen K, Ozorowski G, Burger J, van Montfort T, Stunnenberg M, Labranche C, et al. Presenting native-like HIV-1 envelope trimers on ferritin nanoparticles improves their immunogenicity. Retrovirology. 2015;12:82 pubmed publisher
    ..This or other nanoparticle designs may be practical ways to improve the immunogenicity of envelope glycoprotein trimers. ..
  6. Sliepen K, Medina Ramírez M, Yasmeen A, Moore J, Klasse P, Sanders R. Binding of inferred germline precursors of broadly neutralizing HIV-1 antibodies to native-like envelope trimers. Virology. 2015;486:116-20 pubmed publisher
    ..The data imply that native-like SOSIP.664 trimers will be an appropriate platform for structure-guided design improvements intended to create immunogens able to target the germline precursors of bNAbs. ..
  7. Ringe R, Yasmeen A, Ozorowski G, Go E, Pritchard L, Guttman M, et al. Influences on the Design and Purification of Soluble, Recombinant Native-Like HIV-1 Envelope Glycoprotein Trimers. J Virol. 2015;89:12189-210 pubmed publisher
    ..012 and 92UG037.8 oligomeric Env proteins do not resemble the trimeric Env glycoprotein on HIV-1 viruses, which has implications for the design and interpretation of ongoing or proposed clinical trials of these proteins. ..
  8. Ozorowski G, Cupo A, Golabek M, LoPiccolo M, Ketas T, Cavallary M, et al. Effects of Adjuvants on HIV-1 Envelope Glycoprotein SOSIP Trimers In Vitro. J Virol. 2018;92: pubmed publisher
  9. Moore J, Kuritzkes D. A pièce de resistance: how HIV-1 escapes small molecule CCR5 inhibitors. Curr Opin HIV AIDS. 2009;4:118-24 pubmed publisher
    ..HIV-1 escapes small molecule CCR5 inhibitors by continuing to use CCR5 in an inhibitor-insensitive manner, or evades them by expanding naturally insensitive, CXCR4-using variants. ..

More Information


  1. Ringe R, Ozorowski G, Rantalainen K, Struwe W, Matthews K, Torres J, et al. Reducing V3 Antigenicity and Immunogenicity on Soluble, Native-Like HIV-1 Env SOSIP Trimers. J Virol. 2017;91: pubmed publisher
    ..We characterized the new trimers, tested them as immunogens in rabbits, and found that the blocking glycans eliminated the induction of tier-1 NAbs to V3-epitopes. ..
  2. Ringe R, Ozorowski G, Yasmeen A, Cupo A, Cruz Portillo V, Pugach P, et al. Improving the Expression and Purification of Soluble, Recombinant Native-Like HIV-1 Envelope Glycoprotein Trimers by Targeted Sequence Changes. J Virol. 2017;91: pubmed publisher
    ..012, by using an array of protein engineering techniques to improve a prototypic construct. We also show that the methods may have wider utility for other env genes, thereby further guiding immunogen design. ..