Neal Lue

Summary

Affiliation: Cornell University
Country: USA

Publications

  1. ncbi request reprint Functional analysis of the C-terminal extension of telomerase reverse transcriptase. A putative "thumb" domain
    Shabbir Hossain
    Department of Microbiology and Immunology, William Randolph Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, New York 10021
    J Biol Chem 277:36174-80. 2002
  2. pmc Ever shorter telomere 1 (EST1)-dependent reverse transcription by Candida telomerase in vitro: evidence in support of an activating function
    Sunitha M Singh
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 100:5718-23. 2003
  3. ncbi request reprint Adding to the ends: what makes telomerase processive and how important is it?
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Bioessays 26:955-62. 2004
  4. pmc The telomere capping complex CST has an unusual stoichiometry, makes multipartite interaction with G-Tails, and unfolds higher-order G-tail structures
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, New York, USA
    PLoS Genet 9:e1003145. 2013
  5. doi request reprint A popular engagement at the ends
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, New York, USA
    Nat Struct Mol Biol 20:10-2. 2013
  6. ncbi request reprint Teaching resources. Chromatin remodeling
    Neal F Lue
    Department of Microbiology and Immunology, Weill Medical College of Cornell University, NY 10021, USA
    Sci STKE 2005:tr20. 2005
  7. pmc Telomerase can act as a template- and RNA-independent terminal transferase
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 102:9778-83. 2005
  8. pmc A physical and functional constituent of telomerase anchor site
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, New York 10021, USA
    J Biol Chem 280:26586-91. 2005
  9. pmc Reverse transcriptase at bacterial telomeres
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 101:14307-8. 2004
  10. doi request reprint Closing the feedback loop: how cells "count" telomere-bound proteins
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, NY 10065, USA
    Mol Cell 33:413-4. 2009

Collaborators

Detail Information

Publications26

  1. ncbi request reprint Functional analysis of the C-terminal extension of telomerase reverse transcriptase. A putative "thumb" domain
    Shabbir Hossain
    Department of Microbiology and Immunology, William Randolph Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, New York 10021
    J Biol Chem 277:36174-80. 2002
    ..We suggest that mechanistic similarity between telomerase and retroviral RTs may extend beyond the regions with apparent sequence similarity...
  2. pmc Ever shorter telomere 1 (EST1)-dependent reverse transcription by Candida telomerase in vitro: evidence in support of an activating function
    Sunitha M Singh
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 100:5718-23. 2003
    ....
  3. ncbi request reprint Adding to the ends: what makes telomerase processive and how important is it?
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Bioessays 26:955-62. 2004
    ..Because telomere maintenance and the lack thereof have been linked to tumor progression and aging, further investigation of telomerase processivity may lead to novel medical intervention strategies...
  4. pmc The telomere capping complex CST has an unusual stoichiometry, makes multipartite interaction with G-Tails, and unfolds higher-order G-tail structures
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, New York, USA
    PLoS Genet 9:e1003145. 2013
    ..Our data provide insights on the assembly and mechanisms of CST, and our robust reconstitution system will facilitate future biochemical analysis of this important complex...
  5. doi request reprint A popular engagement at the ends
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, New York, USA
    Nat Struct Mol Biol 20:10-2. 2013
    ..An equivalent interaction appears to exist in budding yeast, making this a nearly universal means of telomerase regulation...
  6. ncbi request reprint Teaching resources. Chromatin remodeling
    Neal F Lue
    Department of Microbiology and Immunology, Weill Medical College of Cornell University, NY 10021, USA
    Sci STKE 2005:tr20. 2005
    ..quot; The lecture begins with a discussion of chromatin organization and then proceeds to describe the process of chromatin remodeling through a review of chromatin remodeling complexes and methods used to study their function...
  7. pmc Telomerase can act as a template- and RNA-independent terminal transferase
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 102:9778-83. 2005
    ..The terminal transferase activity of telomerase may account for some of the hitherto unexplained effects of telomerase overexpression on cell physiology...
  8. pmc A physical and functional constituent of telomerase anchor site
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, New York 10021, USA
    J Biol Chem 280:26586-91. 2005
    ..Coupled with previous genetic analysis, our data confirm that anchor site interaction is indeed important for telomerase function in vivo...
  9. pmc Reverse transcriptase at bacterial telomeres
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 101:14307-8. 2004
  10. doi request reprint Closing the feedback loop: how cells "count" telomere-bound proteins
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, NY 10065, USA
    Mol Cell 33:413-4. 2009
    ..In a recent issue of Molecular Cell, Hirano et al. (2009) delineate the molecular interactions that underlie the budding yeast protein-counting machinery...
  11. pmc Plasticity of telomere maintenance mechanisms in yeast
    Neal F Lue
    Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10065, USA
    Trends Biochem Sci 35:8-17. 2010
    ..Based on these findings, I propose a plausible series of evolutionary events in budding yeast to account for its exceptional telomere structural divergence...
  12. pmc A conserved telomerase motif within the catalytic domain of telomerase reverse transcriptase is specifically required for repeat addition processivity
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, New York 10021, USA
    Mol Cell Biol 23:8440-9. 2003
    ..Our results suggest that the ability to stabilize short RNA-DNA hybrids is crucial for telomerase function in vivo and that this ability is mediated in part by a more elaborate fingers domain structure...
  13. pmc A web of interactions at the ends
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Mol Cell 42:269-71. 2011
    ..In this issue of Molecular Cell, Robart and Collins (2011) describe a set of interactions between human telomerase RNA, protein domains, and the substrate DNA that drives the intricate reaction cycle...
  14. pmc Modeling and structure function analysis of the putative anchor site of yeast telomerase
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Nucleic Acids Res 35:5213-22. 2007
    ..Our data argue that telomerase anchor site has evolved species-specific residues to interact with species-specific telomere repeats. The data also reinforce the importance of telomerase processivity in regulating telomere length...
  15. pmc Analyses of Candida Cdc13 orthologues revealed a novel OB fold dimer arrangement, dimerization-assisted DNA binding, and substantial structural differences between Cdc13 and RPA70
    Eun Young Yu
    Department of Microbiology and Immunology, WR Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, New York, USA
    Mol Cell Biol 32:186-98. 2012
    ..Our findings provide new insights on the mechanisms and evolution of a critical telomere end binding protein...
  16. pmc Analysis of telomerase in Candida albicans: potential role in telomere end protection
    Sunitha M Singh
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, New York 10021, USA
    Eukaryot Cell 1:967-77. 2002
    ..We also identify potential homologues of Est1p in phylogenetically diverse organisms. The Est1p sequence family possesses a conserved N-terminal domain predicted to be structurally related to tetratricopeptide repeat-containing proteins...
  17. pmc Rap1 in Candida albicans: an unusual structural organization and a critical function in suppressing telomere recombination
    Eun Young Yu
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Ave, New York, New York 10021, USA
    Mol Cell Biol 30:1254-68. 2010
    ..Our findings provide insights on the evolution and mechanistic plasticity of a widely conserved and functionally critical telomere component...
  18. pmc A proposed OB-fold with a protein-interaction surface in Candida albicans telomerase protein Est3
    Eun Young Yu
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, New York 10065, USA
    Nat Struct Mol Biol 15:985-9. 2008
    ..We propose that Est3 and the OB-fold domain of Tpp1 mediate a conserved function in telomerase regulation...
  19. pmc Yeast telomerase is capable of limited repeat addition processivity
    Dimitry Bosoy
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Nucleic Acids Res 32:93-101. 2004
    ..Our results suggest that yeast may be a useful system for investigating the physiologic significance of repeat addition processivity...
  20. ncbi request reprint Conserved N-terminal motifs of telomerase reverse transcriptase required for ribonucleoprotein assembly in vivo
    Dimitry Bosoy
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, New York 10021, USA
    J Biol Chem 278:3882-90. 2003
    ..We also show that the majority of telomerase-associated TLC1 RNA has a more upstream 3' end than previously reported, consistent with additional processing events during RNP maturation...
  21. pmc Modulation of telomere terminal structure by telomerase components in Candida albicans
    Olga Steinberg-Neifach
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Nucleic Acids Res 34:2710-22. 2006
    ....
  22. pmc Regulation of telomere structure and functions by subunits of the INO80 chromatin remodeling complex
    Eun Young Yu
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, NY 10021, USA
    Mol Cell Biol 27:5639-49. 2007
    ..Our results reveal novel functions for a subunit of the telomerase complex and the INO80 chromatin remodeling complex...
  23. pmc Telomerase core components protect Candida telomeres from aberrant overhang accumulation
    Min Hsu
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 104:11682-7. 2007
    ..Altogether, our data support the notion that the Candida telomerase core components mediate a protective function through a mechanism that is independent of its catalytic activity...
  24. pmc Mutual dependence of Candida albicans Est1p and Est3p in telomerase assembly and activation
    Min Hsu
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Eukaryot Cell 6:1330-8. 2007
    ..We also developed an affinity isolation and an in vitro reconstitution protocol for the telomerase complex that will facilitate future mechanistic studies...
  25. ncbi request reprint Homologous recombination in Candida albicans: role of CaRad52p in DNA repair, integration of linear DNA fragments and telomere length
    Toni Ciudad
    Departamento de Microbiologia, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, Spain
    Mol Microbiol 53:1177-94. 2004
    ..This raises the possibility that both telomerase and homologous recombination may function simultaneously at C. albicans telomeres...
  26. ncbi request reprint The structure and function of telomerase reverse transcriptase
    Chantal Autexier
    Bloomfield Center for Research in Aging, Lady Davis Institute for Medical Research, Sir Mortimer B Davis Jewish General Hospital, Quebec, Canada
    Annu Rev Biochem 75:493-517. 2006
    ..Here we discuss TERT structure and function; its regulation by RNA-DNA, TERT-DNA, TERT-RNA, TERT-TERT interactions, and TERT-associated proteins; and the relationship between telomerase enzymology and telomere maintenance...

Research Grants14

  1. ANALYSIS OF TELOMERASE REVERSE TRANSCRIPTASE
    Neal F Lue; Fiscal Year: 2010
    ....
  2. ANALYSIS OF TELOMERASE REVERSE TRANSCRIPTASE
    Neal Lue; Fiscal Year: 2009
    ....
  3. Telomerase function and regulation in a new model system
    Neal Lue; Fiscal Year: 2007
    ..We anticipate that our findings will contribute to a detailed molecular understanding of telomerase function and regulation. ..
  4. ANALYSIS OF TELOMERASE REVERSE TRANSCRIPTASE
    Neal Lue; Fiscal Year: 2005
    ..Furthermore, because of the conserved nature of the motifs being analyzed, the findings should provide general insights on telomerase structure and mechanisms. ..
  5. ANALYSIS OF TELOMERASE REVERSE TRANSCRIPTASE
    Neal Lue; Fiscal Year: 2009
    ....