N F Lue

Summary

Affiliation: Cornell University
Country: USA

Publications

  1. pmc Negative regulation of yeast telomerase activity through an interaction with an upstream region of the DNA primer
    N F Lue
    Department of Microbiology, W R Hearst Microbiology Research Center, Cornell University Medical College, 1300 York Avenue, New York, NY 10021, USA
    Nucleic Acids Res 26:1487-94. 1998
  2. pmc Species-specific and sequence-specific recognition of the dG-rich strand of telomeres by yeast telomerase
    N F Lue
    Department of Microbiology, W R Hearst Microbiology Research Center, Cornell University Medical College, 1300 York Avenue, New York, NY 10021, USA
    Nucleic Acids Res 26:1495-502. 1998
  3. pmc Sequence-specific and conformation-dependent binding of yeast telomerase RNA to single-stranded telomeric DNA
    N F Lue
    Department of Microbiology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Nucleic Acids Res 27:2560-7. 1999
  4. pmc Characterization of the interaction between the nuclease and reverse transcriptase activity of the yeast telomerase complex
    H Niu
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, New York 10021, USA
    Mol Cell Biol 20:6806-15. 2000
  5. ncbi request reprint Analysis of telomerase processivity: mechanistic similarity to HIV-1 reverse transcriptase and role in telomere maintenance
    Y Peng
    Department of Microbiology and Immunology, W. R. Hearst Microbiology Research Center, Weill Medical College, Cornell University, 10021, New York, NY, USA
    Mol Cell 7:1201-11. 2001
  6. ncbi request reprint Functional analysis of conserved residues in the putative "finger" domain of telomerase reverse transcriptase
    D Bosoy
    Department of Microbiology and Immunology, William Randolph Hearst Microbiology Research Center, Weill Medical College, Cornell University, New York, New York 10021, USA
    J Biol Chem 276:46305-12. 2001
  7. pmc Telomerase can act as a template- and RNA-independent terminal transferase
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 102:9778-83. 2005
  8. ncbi request reprint Teaching resources. Chromatin remodeling
    Neal F Lue
    Department of Microbiology and Immunology, Weill Medical College of Cornell University, NY 10021, USA
    Sci STKE 2005:tr20. 2005
  9. pmc Modulation of telomere terminal structure by telomerase components in Candida albicans
    Olga Steinberg-Neifach
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Nucleic Acids Res 34:2710-22. 2006
  10. ncbi request reprint The structure and function of telomerase reverse transcriptase
    Chantal Autexier
    Bloomfield Center for Research in Aging, Lady Davis Institute for Medical Research, Sir Mortimer B Davis Jewish General Hospital, Quebec, Canada
    Annu Rev Biochem 75:493-517. 2006

Collaborators

Detail Information

Publications24

  1. pmc Negative regulation of yeast telomerase activity through an interaction with an upstream region of the DNA primer
    N F Lue
    Department of Microbiology, W R Hearst Microbiology Research Center, Cornell University Medical College, 1300 York Avenue, New York, NY 10021, USA
    Nucleic Acids Res 26:1487-94. 1998
    ..A tripartite model of interaction between primer and telomerase is proposed to account for the distinct effects of mutations in different regions of the DNA primer...
  2. pmc Species-specific and sequence-specific recognition of the dG-rich strand of telomeres by yeast telomerase
    N F Lue
    Department of Microbiology, W R Hearst Microbiology Research Center, Cornell University Medical College, 1300 York Avenue, New York, NY 10021, USA
    Nucleic Acids Res 26:1495-502. 1998
    ..The ability of a sequence to be bound tightly by yeast telomerase in vitro correlates with its ability to seed telomere formation in vivo. The implications of these findings for regulation of telomerase activity are discussed...
  3. pmc Sequence-specific and conformation-dependent binding of yeast telomerase RNA to single-stranded telomeric DNA
    N F Lue
    Department of Microbiology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Nucleic Acids Res 27:2560-7. 1999
    ....
  4. pmc Characterization of the interaction between the nuclease and reverse transcriptase activity of the yeast telomerase complex
    H Niu
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, New York 10021, USA
    Mol Cell Biol 20:6806-15. 2000
    ....
  5. ncbi request reprint Analysis of telomerase processivity: mechanistic similarity to HIV-1 reverse transcriptase and role in telomere maintenance
    Y Peng
    Department of Microbiology and Immunology, W. R. Hearst Microbiology Research Center, Weill Medical College, Cornell University, 10021, New York, NY, USA
    Mol Cell 7:1201-11. 2001
    ..A close correlation was uncovered between telomerase processivity and telomere length in vivo, suggesting that enzyme processivity is a limiting factor for telomere maintenance...
  6. ncbi request reprint Functional analysis of conserved residues in the putative "finger" domain of telomerase reverse transcriptase
    D Bosoy
    Department of Microbiology and Immunology, William Randolph Hearst Microbiology Research Center, Weill Medical College, Cornell University, New York, New York 10021, USA
    J Biol Chem 276:46305-12. 2001
    ..This study thus supports a high degree of mechanistic conservation between telomerase and retroviral RTs and underscores the roles of distinct aspects of telomerase biochemistry in telomere length maintenance...
  7. pmc Telomerase can act as a template- and RNA-independent terminal transferase
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 102:9778-83. 2005
    ..The terminal transferase activity of telomerase may account for some of the hitherto unexplained effects of telomerase overexpression on cell physiology...
  8. ncbi request reprint Teaching resources. Chromatin remodeling
    Neal F Lue
    Department of Microbiology and Immunology, Weill Medical College of Cornell University, NY 10021, USA
    Sci STKE 2005:tr20. 2005
    ..quot; The lecture begins with a discussion of chromatin organization and then proceeds to describe the process of chromatin remodeling through a review of chromatin remodeling complexes and methods used to study their function...
  9. pmc Modulation of telomere terminal structure by telomerase components in Candida albicans
    Olga Steinberg-Neifach
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Nucleic Acids Res 34:2710-22. 2006
    ....
  10. ncbi request reprint The structure and function of telomerase reverse transcriptase
    Chantal Autexier
    Bloomfield Center for Research in Aging, Lady Davis Institute for Medical Research, Sir Mortimer B Davis Jewish General Hospital, Quebec, Canada
    Annu Rev Biochem 75:493-517. 2006
    ..Here we discuss TERT structure and function; its regulation by RNA-DNA, TERT-DNA, TERT-RNA, TERT-TERT interactions, and TERT-associated proteins; and the relationship between telomerase enzymology and telomere maintenance...
  11. pmc Mutual dependence of Candida albicans Est1p and Est3p in telomerase assembly and activation
    Min Hsu
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Eukaryot Cell 6:1330-8. 2007
    ..We also developed an affinity isolation and an in vitro reconstitution protocol for the telomerase complex that will facilitate future mechanistic studies...
  12. pmc Regulation of telomere structure and functions by subunits of the INO80 chromatin remodeling complex
    Eun Young Yu
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, NY 10021, USA
    Mol Cell Biol 27:5639-49. 2007
    ..Our results reveal novel functions for a subunit of the telomerase complex and the INO80 chromatin remodeling complex...
  13. pmc Telomerase core components protect Candida telomeres from aberrant overhang accumulation
    Min Hsu
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 104:11682-7. 2007
    ..Altogether, our data support the notion that the Candida telomerase core components mediate a protective function through a mechanism that is independent of its catalytic activity...
  14. pmc A physical and functional constituent of telomerase anchor site
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, New York 10021, USA
    J Biol Chem 280:26586-91. 2005
    ..Coupled with previous genetic analysis, our data confirm that anchor site interaction is indeed important for telomerase function in vivo...
  15. pmc Reverse transcriptase at bacterial telomeres
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 101:14307-8. 2004
  16. ncbi request reprint Adding to the ends: what makes telomerase processive and how important is it?
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Bioessays 26:955-62. 2004
    ..Because telomere maintenance and the lack thereof have been linked to tumor progression and aging, further investigation of telomerase processivity may lead to novel medical intervention strategies...
  17. ncbi request reprint Homologous recombination in Candida albicans: role of CaRad52p in DNA repair, integration of linear DNA fragments and telomere length
    Toni Ciudad
    Departamento de Microbiologia, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, Spain
    Mol Microbiol 53:1177-94. 2004
    ..This raises the possibility that both telomerase and homologous recombination may function simultaneously at C. albicans telomeres...
  18. pmc Yeast telomerase is capable of limited repeat addition processivity
    Dimitry Bosoy
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Nucleic Acids Res 32:93-101. 2004
    ..Our results suggest that yeast may be a useful system for investigating the physiologic significance of repeat addition processivity...
  19. pmc A conserved telomerase motif within the catalytic domain of telomerase reverse transcriptase is specifically required for repeat addition processivity
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, New York 10021, USA
    Mol Cell Biol 23:8440-9. 2003
    ..Our results suggest that the ability to stabilize short RNA-DNA hybrids is crucial for telomerase function in vivo and that this ability is mediated in part by a more elaborate fingers domain structure...
  20. pmc Ever shorter telomere 1 (EST1)-dependent reverse transcription by Candida telomerase in vitro: evidence in support of an activating function
    Sunitha M Singh
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 100:5718-23. 2003
    ....
  21. pmc Analysis of telomerase in Candida albicans: potential role in telomere end protection
    Sunitha M Singh
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, New York 10021, USA
    Eukaryot Cell 1:967-77. 2002
    ..We also identify potential homologues of Est1p in phylogenetically diverse organisms. The Est1p sequence family possesses a conserved N-terminal domain predicted to be structurally related to tetratricopeptide repeat-containing proteins...
  22. ncbi request reprint Conserved N-terminal motifs of telomerase reverse transcriptase required for ribonucleoprotein assembly in vivo
    Dimitry Bosoy
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, New York 10021, USA
    J Biol Chem 278:3882-90. 2003
    ..We also show that the majority of telomerase-associated TLC1 RNA has a more upstream 3' end than previously reported, consistent with additional processing events during RNP maturation...
  23. ncbi request reprint Functional analysis of the C-terminal extension of telomerase reverse transcriptase. A putative "thumb" domain
    Shabbir Hossain
    Department of Microbiology and Immunology, William Randolph Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, New York 10021
    J Biol Chem 277:36174-80. 2002
    ..We suggest that mechanistic similarity between telomerase and retroviral RTs may extend beyond the regions with apparent sequence similarity...
  24. pmc Modeling and structure function analysis of the putative anchor site of yeast telomerase
    Neal F Lue
    Department of Microbiology and Immunology, W R Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Nucleic Acids Res 35:5213-22. 2007
    ..Our data argue that telomerase anchor site has evolved species-specific residues to interact with species-specific telomere repeats. The data also reinforce the importance of telomerase processivity in regulating telomere length...