William Holloman

Summary

Affiliation: Cornell University
Country: USA

Publications

  1. ncbi request reprint The BRCA2-interacting protein DSS1 is vital for DNA repair, recombination, and genome stability in Ustilago maydis
    Milorad Kojic
    Department of Microbiology and Immunology, Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, NY 10021, USA
    Mol Cell 12:1043-9. 2003
  2. pmc Unraveling the mechanism of BRCA2 in homologous recombination
    William K Holloman
    Department of Microbiology and Immunology, Weill Cornell Cancer Center, Weill Cornell Medical College, New York, New York, USA
    Nat Struct Mol Biol 18:748-54. 2011
  3. pmc The homologous recombination system of Ustilago maydis
    William K Holloman
    Cornell University Weill Medical College, Department of Microbiology and Immunology, NY 10021, USA
    Fungal Genet Biol 45:S31-9. 2008
  4. ncbi request reprint Towards understanding the extreme radiation resistance of Ustilago maydis
    William K Holloman
    Cornell University Weill Medical College, Department of Microbiology and Immunology, New York, NY 10021, USA
    Trends Microbiol 15:525-9. 2007
  5. pmc Rec2 interplay with both Brh2 and Rad51 balances recombinational repair in Ustilago maydis
    Milorad Kojic
    Department of Microbiology and Immunology, Hearst Microbiology Research Center, Box 62, Cornell University Weill Medical College, 1300 York Avenue, New York, New York 10021, USA
    Mol Cell Biol 26:678-88. 2006
  6. ncbi request reprint BRCA2 homolog required for proficiency in DNA repair, recombination, and genome stability in Ustilago maydis
    Milorad Kojic
    Department of Microbiology and Immunology, Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, NY 10021, USA
    Mol Cell 10:683-91. 2002
  7. doi request reprint Compensatory role for Rad52 during recombinational repair in Ustilago maydis
    Milorad Kojic
    Department of Microbiology and Immunology, Cornell University Weill Medical College, New York, NY 10065, USA
    Mol Microbiol 67:1156-68. 2008
  8. pmc Second-end capture in DNA double-strand break repair promoted by Brh2 protein of Ustilago maydis
    Nayef Mazloum
    Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY 10021, USA
    Mol Cell 33:160-70. 2009
  9. pmc Dss1 regulates interaction of Brh2 with DNA
    Qingwen Zhou
    Department of Microbiology and Immunology, Weill Cornell Medical College, New York, New York 10065, USA
    Biochemistry 48:11929-38. 2009
  10. pmc Role of Blm and collaborating factors in recombination and survival following replication stress in Ustilago maydis
    Ninghui Mao
    Department of Microbiology and Immunology, Cornell University Weill Medical College, New York, NY 10021, USA
    DNA Repair (Amst) 8:752-9. 2009

Research Grants

  1. DNA Repair Promoted by Rec 2
    William Holloman; Fiscal Year: 2005
  2. BRCA2 model system
    William Holloman; Fiscal Year: 2007
  3. Mechanisms of Brh2-Promoted Recombinatinal Repair
    William Holloman; Fiscal Year: 2007
  4. BRCA2 model system
    William Holloman; Fiscal Year: 2009
  5. Mechanisms of Brh2-Promoted Recombinatinal Repair
    William K Holloman; Fiscal Year: 2010
  6. BRCA2 model system
    William K Holloman; Fiscal Year: 2010
  7. REC1 GENE CONTROLLING RECOMBINATION IN USTILAGO
    William Holloman; Fiscal Year: 1991
  8. FUNCTION OF RECOMBINATION GENES IN USTILAGO MAYDIS
    William Holloman; Fiscal Year: 1993
  9. DNA REPAIR MECHANISMS IN USTILAGO MAYDIS
    William Holloman; Fiscal Year: 1999
  10. BRCA2 model system
    William K Holloman; Fiscal Year: 2010

Collaborators

  • Michael Lisby
  • R Holliday
  • LORRAINE SYMINGTON
  • Milorad Kojic
  • Qingwen Zhou
  • Nayef Mazloum
  • Ninghui Mao
  • Haijuan Yang
  • Nikola P Pavletich
  • Corwin F Kostrub
  • Jose Perez-Martin
  • Nayef A Mazloum
  • Zhimin Cao
  • Jie Fan
  • Qiubai Li
  • Andrew R Buchman

Detail Information

Publications20

  1. ncbi request reprint The BRCA2-interacting protein DSS1 is vital for DNA repair, recombination, and genome stability in Ustilago maydis
    Milorad Kojic
    Department of Microbiology and Immunology, Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, NY 10021, USA
    Mol Cell 12:1043-9. 2003
    ..maydis, and imply that it plays a similar key role in the recombination systems of organisms in which recombinational repair is BRCA2 dependent...
  2. pmc Unraveling the mechanism of BRCA2 in homologous recombination
    William K Holloman
    Department of Microbiology and Immunology, Weill Cornell Cancer Center, Weill Cornell Medical College, New York, New York, USA
    Nat Struct Mol Biol 18:748-54. 2011
    ..This review highlights recent studies on BRCA2 and its orthologs that have begun to illuminate the molecular mechanisms by which these proteins control homologous recombination...
  3. pmc The homologous recombination system of Ustilago maydis
    William K Holloman
    Cornell University Weill Medical College, Department of Microbiology and Immunology, NY 10021, USA
    Fungal Genet Biol 45:S31-9. 2008
    ..These include the use of a BRCA2 homolog and its modifier Dss1 rather than Rad52 as a mediator of Rad51, the presence of only a single Rad51 paralog, and the absence of Dmc1 and auxiliary meiotic proteins...
  4. ncbi request reprint Towards understanding the extreme radiation resistance of Ustilago maydis
    William K Holloman
    Cornell University Weill Medical College, Department of Microbiology and Immunology, New York, NY 10021, USA
    Trends Microbiol 15:525-9. 2007
    ..maydis. Evolution of a system enabling the survival of U. maydis under such conditions could be a secondary consequence of adaptation to an environment of continual genotoxic stress encountered in its habitat...
  5. pmc Rec2 interplay with both Brh2 and Rad51 balances recombinational repair in Ustilago maydis
    Milorad Kojic
    Department of Microbiology and Immunology, Hearst Microbiology Research Center, Box 62, Cornell University Weill Medical College, 1300 York Avenue, New York, New York 10021, USA
    Mol Cell Biol 26:678-88. 2006
    ..The results demonstrate that the importance of Rec2 function is not only in stimulating recombination activity but also in ensuring that recombination is properly controlled...
  6. ncbi request reprint BRCA2 homolog required for proficiency in DNA repair, recombination, and genome stability in Ustilago maydis
    Milorad Kojic
    Department of Microbiology and Immunology, Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, NY 10021, USA
    Mol Cell 10:683-91. 2002
    ..Recapitulation in U. maydis of defects in DNA repair and genome stability associated with brh2 means that the BRCA2 gene family is more widespread than previously thought...
  7. doi request reprint Compensatory role for Rad52 during recombinational repair in Ustilago maydis
    Milorad Kojic
    Department of Microbiology and Immunology, Cornell University Weill Medical College, New York, NY 10065, USA
    Mol Microbiol 67:1156-68. 2008
    ..The results suggest that Rad52 serves in an overlapping, compensatory role with both Rec2 and Brh2 to promote and maintain formation of the Rad51 nucleoprotein filament...
  8. pmc Second-end capture in DNA double-strand break repair promoted by Brh2 protein of Ustilago maydis
    Nayef Mazloum
    Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY 10021, USA
    Mol Cell 33:160-70. 2009
    ....
  9. pmc Dss1 regulates interaction of Brh2 with DNA
    Qingwen Zhou
    Department of Microbiology and Immunology, Weill Cornell Medical College, New York, New York 10065, USA
    Biochemistry 48:11929-38. 2009
    ..Conversely, the Brh2-Dss1 complex dissociates more quickly when DNA is present. These findings suggest a model in which binding of Brh2 to DNA is subject to allosteric regulation by Dss1...
  10. pmc Role of Blm and collaborating factors in recombination and survival following replication stress in Ustilago maydis
    Ninghui Mao
    Department of Microbiology and Immunology, Cornell University Weill Medical College, New York, NY 10021, USA
    DNA Repair (Amst) 8:752-9. 2009
    ....
  11. pmc Dss1 interaction with Brh2 as a regulatory mechanism for recombinational repair
    Qingwen Zhou
    Department of Microbiology and Immunology, Cornell University Weill Medical College, 1300 York Avenue, New York, NY 10021, USA
    Mol Cell Biol 27:2512-26. 2007
    ..We propose that cooperation between BRC and CRE domains and the Dss1-provoked dissociation of Brh2 complexes are requisite features of Brh2's molecular mechanism...
  12. pmc Brh2-Dss1 interplay enables properly controlled recombination in Ustilago maydis
    Milorad Kojic
    Department of Microbiology and Immunology, Box 62, Cornell University Weill Medical College, 1300 York Ave, New York, NY 10021, USA
    Mol Cell Biol 25:2547-57. 2005
    ..The results provide evidence for a mechanism in which Dss1 activates a Brh2-Rad51 complex and balances a finely regulated recombinational repair system...
  13. pmc Ortholog of BRCA2-interacting protein BCCIP controls morphogenetic responses during DNA replication stress in Ustilago maydis
    Ninghui Mao
    Department of Microbiology and Immunology, Hearst Microbiology Research Center, Cornell University, Weill Medical College, New York, NY 10021, USA
    DNA Repair (Amst) 6:1651-60. 2007
    ..These results suggest that Bcp1 could be involved in coordinating morphogenetic events with DNA processing during replication...
  14. pmc DNA binding, annealing, and strand exchange activities of Brh2 protein from Ustilago maydis
    Nayef Mazloum
    Department of Microbiology and Immunology, Cornell University Weill Medical College, New York, New York 10021, USA
    Biochemistry 46:7163-73. 2007
    ..These findings raise the notion that Brh2 may have roles in homologous recombination beyond the previously established Rad51 mediator activity...
  15. pmc DNA-binding Domain within the Brh2 N Terminus Is the Primary Interaction Site for Association with DNA
    Qingwen Zhou
    Department of Microbiology and Immunology, Cornell University Weill Medical College, New York, New York 10021
    J Biol Chem 284:8265-73. 2009
    ..The findings suggest a model in which Brh2(NT) serves as the principal site for association with DNA, while the Brh2(CT) provides a means for regulation...
  16. ncbi request reprint The BRCA2 homologue Brh2 nucleates RAD51 filament formation at a dsDNA-ssDNA junction
    Haijuan Yang
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 433:653-7. 2005
    ..These results establish a BRCA2 function in RAD51-mediated DSB repair and explain the loss of this repair capacity in BRCA2-associated cancers...
  17. pmc Brh2 promotes a template-switching reaction enabling recombinational bypass of lesions during DNA synthesis
    Nayef Mazloum
    Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY 10065, USA
    Mol Cell 36:620-30. 2009
    ..We propose that template switching promoted by Brh2 provides a mechanism for recombination-mediated bypass of lesions blocking synthesis during DNA replication...
  18. pmc D-loop formation by Brh2 protein of Ustilago maydis
    Nayef Mazloum
    Department of Microbiology and Immunology, Cornell University Weill Medical College, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 105:524-9. 2008
    ..However, the mutant protein was unable to enhance the Rad51-catalyzed reaction. The results suggest a model in which Brh2 binding to plasmid DNA attracts and helps capture Rad51-coated ssDNA...
  19. ncbi request reprint Molecular biology. New Year's resolution--resolving resolvases
    Lorraine S Symington
    Department of Microbiology and Institute of Cancer Research, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Science 303:184-5. 2004
  20. ncbi request reprint BRCA2-RAD51-DSS1 interplay examined from a microbial perspective
    Milorad Kojic
    Department of Microbiology and Immunology, Hearst Microbiology Research Center, Cornell University Weill Medical College, New York, New York 10021, USA
    Cell Cycle 3:247-8. 2004
    ..A model fungal system that reproduces the BRCA2-RAD51 interaction offers the opportunity to understand the mechanism of DSS1 activation at the molecular level...

Research Grants24

  1. DNA Repair Promoted by Rec 2
    William Holloman; Fiscal Year: 2005
    ..We believe that the studies proposed here will provide novel insight into the basis of homologous pairing processes and provide new insight into the function of a gene important in human breast cancer. ..
  2. BRCA2 model system
    William Holloman; Fiscal Year: 2007
    ..The assembly of such knowledge is the real precursor to breast cancer therapy and cure. ..
  3. Mechanisms of Brh2-Promoted Recombinatinal Repair
    William Holloman; Fiscal Year: 2007
    ..The research program proposed here should bring new light on the molecular mechanism of action of Brh2, and by inference, BRCA2. The assembly of such knowledge is the real precursor to breast cancer therapy and cure. ..
  4. BRCA2 model system
    William Holloman; Fiscal Year: 2009
    ..The assembly of such knowledge is the real precursor to breast cancer therapy and cure. ..
  5. Mechanisms of Brh2-Promoted Recombinatinal Repair
    William K Holloman; Fiscal Year: 2010
    ..The research program proposed here should bring new light on the molecular mechanism of action of Brh2, and by inference, BRCA2. The assembly of such knowledge is the real precursor to breast cancer therapy and cure. ..
  6. BRCA2 model system
    William K Holloman; Fiscal Year: 2010
    ..This is a proposal to learn about the fundamental action of how BRCA2 works in regulating homologous recombination by studying a model microbial system. ..
  7. REC1 GENE CONTROLLING RECOMBINATION IN USTILAGO
    William Holloman; Fiscal Year: 1991
    ..Finally, the gene will be separated from its natural promoter and placed under control of a different promoter sequence in order for us to study the biological consequences of heterologous control...
  8. FUNCTION OF RECOMBINATION GENES IN USTILAGO MAYDIS
    William Holloman; Fiscal Year: 1993
    ..Proposed studies include further experimentation to explore the function of the proteins and to dissect the transcription units of the genes...
  9. DNA REPAIR MECHANISMS IN USTILAGO MAYDIS
    William Holloman; Fiscal Year: 1999
    ..The aim is to identify likely interactors and to isolate mutants in these proteins by the usual reverse genetics approaches. It is anticipated that this may identify other genes that play a role in REC1-dependent activities. ..
  10. BRCA2 model system
    William K Holloman; Fiscal Year: 2010
    ..The assembly of such knowledge is the real precursor to breast cancer therapy and cure. ..