William HollomanSummaryAffiliation: Cornell University Country: USA Publications
Research Grants
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Publications
The BRCA2-interacting protein DSS1 is vital for DNA repair, recombination, and genome stability in Ustilago maydisMilorad Kojic
Department of Microbiology and Immunology, Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, NY 10021, USA
Mol Cell 12:1043-9. 2003..maydis, and imply that it plays a similar key role in the recombination systems of organisms in which recombinational repair is BRCA2 dependent...
Unraveling the mechanism of BRCA2 in homologous recombinationWilliam K Holloman
Department of Microbiology and Immunology, Weill Cornell Cancer Center, Weill Cornell Medical College, New York, New York, USA
Nat Struct Mol Biol 18:748-54. 2011..This review highlights recent studies on BRCA2 and its orthologs that have begun to illuminate the molecular mechanisms by which these proteins control homologous recombination...
The homologous recombination system of Ustilago maydisWilliam K Holloman
Cornell University Weill Medical College, Department of Microbiology and Immunology, NY 10021, USA
Fungal Genet Biol 45:S31-9. 2008..These include the use of a BRCA2 homolog and its modifier Dss1 rather than Rad52 as a mediator of Rad51, the presence of only a single Rad51 paralog, and the absence of Dmc1 and auxiliary meiotic proteins...- Towards understanding the extreme radiation resistance of Ustilago maydisWilliam K Holloman
Cornell University Weill Medical College, Department of Microbiology and Immunology, New York, NY 10021, USA
Trends Microbiol 15:525-9. 2007..maydis. Evolution of a system enabling the survival of U. maydis under such conditions could be a secondary consequence of adaptation to an environment of continual genotoxic stress encountered in its habitat... - Rec2 interplay with both Brh2 and Rad51 balances recombinational repair in Ustilago maydisMilorad Kojic
Department of Microbiology and Immunology, Hearst Microbiology Research Center, Box 62, Cornell University Weill Medical College, 1300 York Avenue, New York, New York 10021, USA
Mol Cell Biol 26:678-88. 2006..The results demonstrate that the importance of Rec2 function is not only in stimulating recombination activity but also in ensuring that recombination is properly controlled... - BRCA2 homolog required for proficiency in DNA repair, recombination, and genome stability in Ustilago maydisMilorad Kojic
Department of Microbiology and Immunology, Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, NY 10021, USA
Mol Cell 10:683-91. 2002..Recapitulation in U. maydis of defects in DNA repair and genome stability associated with brh2 means that the BRCA2 gene family is more widespread than previously thought... - Compensatory role for Rad52 during recombinational repair in Ustilago maydisMilorad Kojic
Department of Microbiology and Immunology, Cornell University Weill Medical College, New York, NY 10065, USA
Mol Microbiol 67:1156-68. 2008..The results suggest that Rad52 serves in an overlapping, compensatory role with both Rec2 and Brh2 to promote and maintain formation of the Rad51 nucleoprotein filament... - Second-end capture in DNA double-strand break repair promoted by Brh2 protein of Ustilago maydisNayef Mazloum
Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY 10021, USA
Mol Cell 33:160-70. 2009.... - Dss1 regulates interaction of Brh2 with DNAQingwen Zhou
Department of Microbiology and Immunology, Weill Cornell Medical College, New York, New York 10065, USA
Biochemistry 48:11929-38. 2009..Conversely, the Brh2-Dss1 complex dissociates more quickly when DNA is present. These findings suggest a model in which binding of Brh2 to DNA is subject to allosteric regulation by Dss1... - Role of Blm and collaborating factors in recombination and survival following replication stress in Ustilago maydisNinghui Mao
Department of Microbiology and Immunology, Cornell University Weill Medical College, New York, NY 10021, USA
DNA Repair (Amst) 8:752-9. 2009.... - Dss1 interaction with Brh2 as a regulatory mechanism for recombinational repairQingwen Zhou
Department of Microbiology and Immunology, Cornell University Weill Medical College, 1300 York Avenue, New York, NY 10021, USA
Mol Cell Biol 27:2512-26. 2007..We propose that cooperation between BRC and CRE domains and the Dss1-provoked dissociation of Brh2 complexes are requisite features of Brh2's molecular mechanism... - Brh2-Dss1 interplay enables properly controlled recombination in Ustilago maydisMilorad Kojic
Department of Microbiology and Immunology, Box 62, Cornell University Weill Medical College, 1300 York Ave, New York, NY 10021, USA
Mol Cell Biol 25:2547-57. 2005..The results provide evidence for a mechanism in which Dss1 activates a Brh2-Rad51 complex and balances a finely regulated recombinational repair system... - Ortholog of BRCA2-interacting protein BCCIP controls morphogenetic responses during DNA replication stress in Ustilago maydisNinghui Mao
Department of Microbiology and Immunology, Hearst Microbiology Research Center, Cornell University, Weill Medical College, New York, NY 10021, USA
DNA Repair (Amst) 6:1651-60. 2007..These results suggest that Bcp1 could be involved in coordinating morphogenetic events with DNA processing during replication... - DNA binding, annealing, and strand exchange activities of Brh2 protein from Ustilago maydisNayef Mazloum
Department of Microbiology and Immunology, Cornell University Weill Medical College, New York, New York 10021, USA
Biochemistry 46:7163-73. 2007..These findings raise the notion that Brh2 may have roles in homologous recombination beyond the previously established Rad51 mediator activity... - DNA-binding Domain within the Brh2 N Terminus Is the Primary Interaction Site for Association with DNAQingwen Zhou
Department of Microbiology and Immunology, Cornell University Weill Medical College, New York, New York 10021
J Biol Chem 284:8265-73. 2009..The findings suggest a model in which Brh2(NT) serves as the principal site for association with DNA, while the Brh2(CT) provides a means for regulation... - The BRCA2 homologue Brh2 nucleates RAD51 filament formation at a dsDNA-ssDNA junctionHaijuan Yang
Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA
Nature 433:653-7. 2005..These results establish a BRCA2 function in RAD51-mediated DSB repair and explain the loss of this repair capacity in BRCA2-associated cancers... - Brh2 promotes a template-switching reaction enabling recombinational bypass of lesions during DNA synthesisNayef Mazloum
Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY 10065, USA
Mol Cell 36:620-30. 2009..We propose that template switching promoted by Brh2 provides a mechanism for recombination-mediated bypass of lesions blocking synthesis during DNA replication... - D-loop formation by Brh2 protein of Ustilago maydisNayef Mazloum
Department of Microbiology and Immunology, Cornell University Weill Medical College, New York, NY 10065, USA
Proc Natl Acad Sci U S A 105:524-9. 2008..However, the mutant protein was unable to enhance the Rad51-catalyzed reaction. The results suggest a model in which Brh2 binding to plasmid DNA attracts and helps capture Rad51-coated ssDNA... - Molecular biology. New Year's resolution--resolving resolvasesLorraine S Symington
Department of Microbiology and Institute of Cancer Research, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
Science 303:184-5. 2004 - BRCA2-RAD51-DSS1 interplay examined from a microbial perspectiveMilorad Kojic
Department of Microbiology and Immunology, Hearst Microbiology Research Center, Cornell University Weill Medical College, New York, New York 10021, USA
Cell Cycle 3:247-8. 2004..A model fungal system that reproduces the BRCA2-RAD51 interaction offers the opportunity to understand the mechanism of DSS1 activation at the molecular level...
Research Grants
- DNA Repair Promoted by Rec 2William Holloman; Fiscal Year: 2005..We believe that the studies proposed here will provide novel insight into the basis of homologous pairing processes and provide new insight into the function of a gene important in human breast cancer. ..
- BRCA2 model systemWilliam Holloman; Fiscal Year: 2007..The assembly of such knowledge is the real precursor to breast cancer therapy and cure. ..
- Mechanisms of Brh2-Promoted Recombinatinal RepairWilliam Holloman; Fiscal Year: 2007..The research program proposed here should bring new light on the molecular mechanism of action of Brh2, and by inference, BRCA2. The assembly of such knowledge is the real precursor to breast cancer therapy and cure. ..
- BRCA2 model systemWilliam Holloman; Fiscal Year: 2009..The assembly of such knowledge is the real precursor to breast cancer therapy and cure. ..
- Mechanisms of Brh2-Promoted Recombinatinal RepairWilliam K Holloman; Fiscal Year: 2010..The research program proposed here should bring new light on the molecular mechanism of action of Brh2, and by inference, BRCA2. The assembly of such knowledge is the real precursor to breast cancer therapy and cure. ..
- BRCA2 model systemWilliam K Holloman; Fiscal Year: 2010..This is a proposal to learn about the fundamental action of how BRCA2 works in regulating homologous recombination by studying a model microbial system. ..
- REC1 GENE CONTROLLING RECOMBINATION IN USTILAGOWilliam Holloman; Fiscal Year: 1991..Finally, the gene will be separated from its natural promoter and placed under control of a different promoter sequence in order for us to study the biological consequences of heterologous control...
- FUNCTION OF RECOMBINATION GENES IN USTILAGO MAYDISWilliam Holloman; Fiscal Year: 1993..Proposed studies include further experimentation to explore the function of the proteins and to dissect the transcription units of the genes...
- DNA REPAIR MECHANISMS IN USTILAGO MAYDISWilliam Holloman; Fiscal Year: 1999..The aim is to identify likely interactors and to isolate mutants in these proteins by the usual reverse genetics approaches. It is anticipated that this may identify other genes that play a role in REC1-dependent activities. ..
- BRCA2 model systemWilliam K Holloman; Fiscal Year: 2010..The assembly of such knowledge is the real precursor to breast cancer therapy and cure. ..