Rey Huei Chen

Summary

Affiliation: Cornell University
Country: USA

Publications

  1. ncbi The spindle checkpoint in Xenopus laevis
    Rey Huei Chen
    Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan
    Front Biosci 13:2231-7. 2008
  2. ncbi Spindle checkpoint protein Bub1 is required for kinetochore localization of Mad1, Mad2, Bub3, and CENP-E, independently of its kinase activity
    H Sharp-Baker
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    J Cell Biol 153:1239-50. 2001
  3. ncbi Spindle checkpoint protein Xmad1 recruits Xmad2 to unattached kinetochores
    R H Chen
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    J Cell Biol 143:283-95. 1998
  4. ncbi The spindle checkpoint of budding yeast depends on a tight complex between the Mad1 and Mad2 proteins
    R H Chen
    Department of Physiology, University of California, San Francisco, San Francisco, California 94143 0444, USA
    Mol Biol Cell 10:2607-18. 1999
  5. ncbi BubR1 is essential for kinetochore localization of other spindle checkpoint proteins and its phosphorylation requires Mad1
    Rey Huei Chen
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    J Cell Biol 158:487-96. 2002
  6. ncbi Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae
    Jing Pan
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Genes Dev 18:1439-51. 2004
  7. ncbi Phosphorylation and activation of Bub1 on unattached chromosomes facilitate the spindle checkpoint
    Rey Huei Chen
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA
    EMBO J 23:3113-21. 2004
  8. ncbi Spindle checkpoint requires Mad1-bound and Mad1-free Mad2
    Eunah Chung
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Mol Biol Cell 13:1501-11. 2002
  9. ncbi Phosphorylation of Cdc20 is required for its inhibition by the spindle checkpoint
    Eunah Chung
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Nat Cell Biol 5:748-53. 2003
  10. ncbi Mps1 phosphorylation by MAP kinase is required for kinetochore localization of spindle-checkpoint proteins
    Yong Zhao
    Graduate Field of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    Curr Biol 16:1764-9. 2006

Research Grants

  1. SPINDLE ASSEMBLY CHECKPOINT IN VERTEBRATES AND YEAST
    Rey Huei Chen; Fiscal Year: 2003

Collaborators

Detail Information

Publications12

  1. ncbi The spindle checkpoint in Xenopus laevis
    Rey Huei Chen
    Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan
    Front Biosci 13:2231-7. 2008
    ..This article reviews the progress of the spindle checkpoint studies in Xenopus laevis with a focus on the regulation by phosphorylation...
  2. ncbi Spindle checkpoint protein Bub1 is required for kinetochore localization of Mad1, Mad2, Bub3, and CENP-E, independently of its kinase activity
    H Sharp-Baker
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    J Cell Biol 153:1239-50. 2001
    ..Our studies demonstrate that Bub1 plays a central role in triggering the spindle checkpoint signal from the kinetochore, and that its kinase activity is not necessary for the spindle checkpoint in Xenopus egg extracts...
  3. ncbi Spindle checkpoint protein Xmad1 recruits Xmad2 to unattached kinetochores
    R H Chen
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    J Cell Biol 143:283-95. 1998
    ..In the presence of excess Xmad2, neither chromosomes nor Xmad1 are required to activate the spindle checkpoint, suggesting that the physiological role of Xmad1 is to recruit Xmad2 to kinetochores that have not bound microtubules...
  4. ncbi The spindle checkpoint of budding yeast depends on a tight complex between the Mad1 and Mad2 proteins
    R H Chen
    Department of Physiology, University of California, San Francisco, San Francisco, California 94143 0444, USA
    Mol Biol Cell 10:2607-18. 1999
    ..Deletion and mutational analysis of both proteins indicate that association of Mad2p with Mad1p is critical for checkpoint function and for hyperphosphorylation of Mad1p...
  5. ncbi BubR1 is essential for kinetochore localization of other spindle checkpoint proteins and its phosphorylation requires Mad1
    Rey Huei Chen
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    J Cell Biol 158:487-96. 2002
    ..This paper demonstrates that BubR1 plays an important role in kinetochore association of other spindle checkpoint proteins and that Mad1 facilitates BubR1 hyperphosphorylation at kinetochores...
  6. ncbi Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae
    Jing Pan
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Genes Dev 18:1439-51. 2004
    ..Our study suggests that the spindle checkpoint reduces Cdc20p to below a certain threshold level to ensure a complete inhibition of Cdc20p before anaphase...
  7. ncbi Phosphorylation and activation of Bub1 on unattached chromosomes facilitate the spindle checkpoint
    Rey Huei Chen
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA
    EMBO J 23:3113-21. 2004
    ....
  8. ncbi Spindle checkpoint requires Mad1-bound and Mad1-free Mad2
    Eunah Chung
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Mol Biol Cell 13:1501-11. 2002
    ..We propose that Mad2 may become activated and dissociated from Mad1 at kinetochores and is replenished by the pool of Mad1-free Mad2...
  9. ncbi Phosphorylation of Cdc20 is required for its inhibition by the spindle checkpoint
    Eunah Chung
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Nat Cell Biol 5:748-53. 2003
    ..This study demonstrates that the spindle checkpoint stops anaphase by inhibiting fully-phosphorylated Cdc20. Our results also have implications for the spindle checkpoint silencing mechanism...
  10. ncbi Mps1 phosphorylation by MAP kinase is required for kinetochore localization of spindle-checkpoint proteins
    Yong Zhao
    Graduate Field of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    Curr Biol 16:1764-9. 2006
    ..In addition, our results indicate that active Mps1 must localize to kinetochores in order to execute its checkpoint function...
  11. ncbi Dual inhibition of Cdc20 by the spindle checkpoint
    Rey Huei Chen
    Institute of Molecular Biology, Academia Sinica, Taipei, 11529, Taiwan
    J Biomed Sci 14:475-9. 2007
    ..This dual inhibition on Cdc20 likely ensures that the spindle checkpoint is sustained even when the cell contains only a single unattached kinetochore...
  12. ncbi Molecular interpretation of ERK signal duration by immediate early gene products
    Leon O Murphy
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Nat Cell Biol 4:556-64. 2002
    ..Together, our data identify a general mechanism by which cells can interpret differences in ERK activation kinetics...

Research Grants6

  1. SPINDLE ASSEMBLY CHECKPOINT IN VERTEBRATES AND YEAST
    Rey Huei Chen; Fiscal Year: 2003
    ..The knowledge will then be applied to the question of how the deregulation of the checkpoint function may contribute to cancer progression. ..