Yung Fu Chang

Summary

Affiliation: Cornell University
Country: USA

Publications

  1. ncbi request reprint Immunogenicity of the recombinant leptospiral putative outer membrane proteins as vaccine candidates
    Yung Fu Chang
    College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
    Vaccine 25:8190-7. 2007
  2. pmc Phenotypic and transcriptomic response of auxotrophic Mycobacterium avium subsp. paratuberculosis leuD mutant under environmental stress
    Jenn Wei Chen
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, United States of America
    PLoS ONE 7:e37884. 2012
  3. pmc Temporal differential proteomes of Clostridium difficile in the pig ileal-ligated loop model
    Tavan Janvilisri
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA
    PLoS ONE 7:e45608. 2012
  4. pmc Analysis of ultra low genome conservation in Clostridium difficile
    Joy Scaria
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, United States of America
    PLoS ONE 5:e15147. 2010
  5. pmc The C-terminal variable domain of LigB from Leptospira mediates binding to fibronectin
    Yi Pin Lin
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    J Vet Sci 9:133-44. 2008
  6. pmc The terminal immunoglobulin-like repeats of LigA and LigB of Leptospira enhance their binding to gelatin binding domain of fibronectin and host cells
    Yi Pin Lin
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, United States of America
    PLoS ONE 5:e11301. 2010
  7. pmc In vitro susceptibilities of Leptospira spp. and Borrelia burgdorferi isolates to amoxicillin, tilmicosin, and enrofloxacin
    Doo Kim
    College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    J Vet Sci 7:355-9. 2006
  8. pmc Differential stress transcriptome landscape of historic and recently emerged hypervirulent strains of Clostridium difficile strains determined using RNA-seq
    Joy Scaria
    Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York, United States of America
    PLoS ONE 8:e78489. 2013
  9. pmc Clonal dissemination of the multi-drug resistant Salmonella enterica serovar Braenderup, but not the serovar Bareilly, of prevalent serogroup C1 Salmonella from Taiwan
    Chien Shun Chiou
    The Central Region Laboratory, Center of Research and Diagnostics, Centers for Disease Control, No 30, Wenxin S 3rd Rd, Nantun Dist, Taichung, 40856, Taiwan
    BMC Microbiol 9:264. 2009
  10. pmc Analysis of Escherichia coli O157 clinical isolates by multilocus sequence typing
    Swaraj Rajkhowa
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
    BMC Res Notes 3:343. 2010

Collaborators

Detail Information

Publications83

  1. ncbi request reprint Immunogenicity of the recombinant leptospiral putative outer membrane proteins as vaccine candidates
    Yung Fu Chang
    College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
    Vaccine 25:8190-7. 2007
    ..Our results indicate that rLp1454, rLp1118, and rMceII showed protection individually and synergistically against serovar Pomona infection, which may help us to develop a multicomponent vaccine for leptospirosis...
  2. pmc Phenotypic and transcriptomic response of auxotrophic Mycobacterium avium subsp. paratuberculosis leuD mutant under environmental stress
    Jenn Wei Chen
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, United States of America
    PLoS ONE 7:e37884. 2012
    ..Our results therefore reveal the intricate connection between the metabolism and virulence in MAP...
  3. pmc Temporal differential proteomes of Clostridium difficile in the pig ileal-ligated loop model
    Tavan Janvilisri
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA
    PLoS ONE 7:e45608. 2012
    ..difficile that demonstrates time-dependent protein expression changes under conditions that mimic in vivo infection and identifies potential candidates for diagnostic or therapeutic measures...
  4. pmc Analysis of ultra low genome conservation in Clostridium difficile
    Joy Scaria
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, United States of America
    PLoS ONE 5:e15147. 2010
    ..However, few pathways such as tetrahydrofolate biosynthesis were found to be variable and could be contributing to adaptation towards virulence such as antibiotic resistance...
  5. pmc The C-terminal variable domain of LigB from Leptospira mediates binding to fibronectin
    Yi Pin Lin
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    J Vet Sci 9:133-44. 2008
    ..Taken together, the data from the present study showed that LigB is a Fn-binding protein of pathogenic Leptospira spp. and may play a pivotal role in Leptospira-host interaction during the initial stage of infection...
  6. pmc The terminal immunoglobulin-like repeats of LigA and LigB of Leptospira enhance their binding to gelatin binding domain of fibronectin and host cells
    Yi Pin Lin
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, United States of America
    PLoS ONE 5:e11301. 2010
    ..This is the first report about the interaction of gelatin binding domain of Fn and Lig proteins and provides an example of Lig-gelatin binding domain binding mediating bacterial-host interaction...
  7. pmc In vitro susceptibilities of Leptospira spp. and Borrelia burgdorferi isolates to amoxicillin, tilmicosin, and enrofloxacin
    Doo Kim
    College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    J Vet Sci 7:355-9. 2006
    ..All spirochetal isolates were inhibited (MIC) and were killed (MBC) by tilmicosin at concentrations below the limit of testing (<or=0.01 microgram/ml)...
  8. pmc Differential stress transcriptome landscape of historic and recently emerged hypervirulent strains of Clostridium difficile strains determined using RNA-seq
    Joy Scaria
    Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York, United States of America
    PLoS ONE 8:e78489. 2013
    ..We also detected a number of transcriptionally active regions that were not part of the primary genome annotation. Some of these are likely to be small regulatory RNAs. ..
  9. pmc Clonal dissemination of the multi-drug resistant Salmonella enterica serovar Braenderup, but not the serovar Bareilly, of prevalent serogroup C1 Salmonella from Taiwan
    Chien Shun Chiou
    The Central Region Laboratory, Center of Research and Diagnostics, Centers for Disease Control, No 30, Wenxin S 3rd Rd, Nantun Dist, Taichung, 40856, Taiwan
    BMC Microbiol 9:264. 2009
    ..Bareilly) and Braenderup (S. Braenderup) were compared, and multi-drug resistance (MDR) plasmids were characterized...
  10. pmc Analysis of Escherichia coli O157 clinical isolates by multilocus sequence typing
    Swaraj Rajkhowa
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
    BMC Res Notes 3:343. 2010
    ..This method relies on an unambiguous DNA sequence analysis of nucleotide polymorphisms in housekeeping genes and has shown a high degree of intraspecies discriminatory power for bacterial and fungal pathogens...
  11. ncbi request reprint Experimental infection of the human granulocytic ehrlichiosis agent in horses
    Y F Chang
    Diagnostic Laboratory, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Vet Parasitol 78:137-45. 1998
    ..Since HGE and E. equi cause very similar diseases in both humans and horses, they may be the same organism with minor genetic differences...
  12. ncbi request reprint Antibiotic treatment of experimentally Borrelia burgdorferi-infected ponies
    Yung Fu Chang
    College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Vet Microbiol 107:285-94. 2005
    ..However, all four-tetracycline treatment ponies showed a negative antibody level and culture negative from post-mortem tissues. Untreated infected ponies maintained high KELA titers throughout the study and were tissue culture positive...
  13. pmc Microbial Diagnostic Array Workstation (MDAW): a web server for diagnostic array data storage, sharing and analysis
    Joy Scaria
    Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York 14853, USA
    Source Code Biol Med 3:14. 2008
    ..Hence we developed a new online data sharing and analysis environment customised for diagnostic arrays...
  14. ncbi request reprint Vaccination against lyme disease with recombinant Borrelia burgdorferi outer-surface protein A (rOspA) in horses
    Y Chang
    Department of Population Medicine and Diagnostic Science, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Vaccine 18:540-8. 1999
    ..Results from this study show that vaccination with recombinant OspA protected ponies against infection after experimental challenge with B. burgdorferi-infected ticks...
  15. pmc Human granulocytic ehrlichiosis agent infection in a pony vaccinated with a Borrelia burgdorferi recombinant OspA vaccine and challenged by exposure to naturally infected ticks
    Y F Chang
    Department of Population Medicine and Diagnostic Science, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
    Clin Diagn Lab Immunol 7:68-71. 2000
    ..burgdorferi infection. These results highlight the potential for ticks to harbor and transmit several pathogens simultaneously, which further complicates the diagnosis and vaccination of these emerging tick-borne diseases...
  16. ncbi request reprint Experimental infection of ponies with Borrelia burgdorferi by exposure to Ixodid ticks
    Y F Chang
    Department of Population Medicine and Diagnostic Science, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Vet Pathol 37:68-76. 2000
    ..burgdorferi-infected ticks. This Lyme disease model will be very useful to evaluate efficacy of vaccines against the Lyme agent and the effect of antibiotic therapy on horses infected with B. burgdorferi...
  17. ncbi request reprint The isolation and sequence of canine interleukin-8 receptor
    Y F Chang
    Department of Population Medicine and Diagnostic Science, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    DNA Seq 10:183-7. 1999
    ..The derived protein from the cDNA sequences shows 75.3%/70.3%, 75.3%/70.1%, 74.8%/69.4%, 70%/59%, and 69.7% identity to that of human, rabbit, gorilla, rat and mouse IL8RB/IL8RA homolog...
  18. doi request reprint Identification and characterization of OmpA-like proteins as novel vaccine candidates for Leptospirosis
    WeiWei Yan
    College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Vaccine 28:2277-83. 2010
    ..In contrast, control animals injected with PBS demonstrated low survival and had significant lesions. All these results clearly indicate that these three OmpA-like proteins may serve as novel vaccine candidates for leptospirosis...
  19. doi request reprint Vaccination with recombinant Mycobacterium avium subsp. paratuberculosis proteins induces differential immune responses and protects calves against infection by oral challenge
    Kumanan Kathaperumal
    Animal Health Diagnostic Center, Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Vaccine 26:1652-63. 2008
    ..Preliminary data from this study indicates that all four rAgs induced a good Th1 response and conferred protection against MAP infection in calves...
  20. doi request reprint Immunogenicity and protective efficacy of the Mycobacterium avium subsp. paratuberculosis attenuated mutants against challenge in a mouse model
    Jenn Wei Chen
    Animal Health Diagnostic Center, Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Vaccine 30:3015-25. 2012
    ..In addition, vaccination with leuD mutant induced a high level of IFN-γ production and significant protective efficacy in both the reduction of inflammation and clearance of acid-fast bacilli, as compared with the mock vaccinated group...
  21. doi request reprint Immune responses in mice to Mycobacterium avium subsp. paratuberculosis following vaccination with a novel 74F recombinant polyprotein
    Li Hsuen Chen
    College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Vaccine 26:1253-62. 2008
    ..Results of this study indicated that immunization of mice with Map74F protected mice against MAP infection...
  22. ncbi request reprint Evaluation of protective immunity of Leptospira immunoglobulin like protein A (LigA) DNA vaccine against challenge in hamsters
    Syed M Faisal
    College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Vaccine 26:277-87. 2008
    ..Taken together, the present study suggests that a LigA DNA vaccine is a promising candidate for prevention of leptospirosis...
  23. ncbi request reprint Immune response and prophylactic efficacy of smegmosomes in a hamster model of leptospirosis
    Syed M Faisal
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, United States
    Vaccine 27:6129-36. 2009
    ....
  24. doi request reprint Leptospira immunoglobulin-like protein A variable region (LigAvar) incorporated in liposomes and PLGA microspheres produces a robust immune response correlating to protective immunity
    Syed M Faisal
    Animal Health Diagnostic Center, Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Vaccine 27:378-87. 2009
    ..Taken together, the data of the present study suggests that both liposomes and PLGA microspheres are promising adjuvants for use with future subunit vaccines for prevention of leptospirosis...
  25. ncbi request reprint Expression of leptospiral immunoglobulin-like protein by Leptospira interrogans and evaluation of its diagnostic potential in a kinetic ELISA
    Raghavan U M Palaniappan
    College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    J Med Microbiol 53:975-84. 2004
    ..These findings suggest that recombinant antigen to the conserved region of LigA and LigB can differentiate between vaccinated and naturally infected animals...
  26. doi request reprint Leptosome-entrapped leptospiral antigens conferred significant higher levels of protection than those entrapped with PC-liposomes in a hamster model
    Syed M Faisal
    College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Vaccine 27:6537-45. 2009
    ....
  27. pmc Immunoprotection of recombinant leptospiral immunoglobulin-like protein A against Leptospira interrogans serovar Pomona infection
    Raghavan U M Palaniappan
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Infect Immun 74:1745-50. 2006
    ..All vaccinated animals showed a rise in antibody titers against rLigA. Results from this study indicate that rLigA is a potential vaccine candidate against L. interrogans serovar Pomona infection...
  28. ncbi request reprint Comparative antibody response of five recombinant antigens in related to bacterial shedding levels and development of serological diagnosis based on 35 kDa antigen for Mycobacterium avium subsp. paratuberculosis
    Sung Jae Shin
    College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    J Vet Sci 5:111-7. 2004
    ..The sera, which showed difference in the comparison with commercial ELISA kit, they also did not react with 35 kDa in Western blot. These results suggest that a 35-kDa based ELISA can be useful for detecting MPT infection...
  29. pmc Evaluation of a Salmonella vectored vaccine expressing Mycobacterium avium subsp. paratuberculosis antigens against challenge in a goat model
    Syed M Faisal
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA
    PLoS ONE 8:e70171. 2013
    ..Hence the Sal-Ag vaccine needs further refinement to increase its efficacy. ..
  30. ncbi request reprint Cloning and characterization of an outer membrane protein (Lip18) from Helicobacter bizzozeronii
    Jiaqian Zhu
    College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    DNA Seq 14:87-94. 2003
    ..The Lip18 was localized into the outer membrane of the bacteria. Immunoblot analysis of serum samples from a dog and cat naturally infected with Helicobacter spp was able to recognize the purified recombinant Lip18...
  31. doi request reprint Immunization with a DNA vaccine cocktail induces a Th1 response and protects mice against Mycobacterium avium subsp. paratuberculosis challenge
    Sung Un Park
    College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Vaccine 26:4329-37. 2008
    ..These results indicate that the use of a recombinant DNA vaccine can provide protective immunity against mycobacterial infection by inducing a Th1 response...
  32. ncbi request reprint Evaluation of polymerase chain reaction diagnosis of Cryptosporidium spp in dairy cattle and wildlife
    Peter E Ziegler
    Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY 14853 3565, USA
    Vet Ther 8:148-59. 2007
    ..Although PCR can be both highly sensitive and accurate, the ability of these protocols to accurately detect DNA in samples can vary. A combination of techniques may be the best choice for to screen samples for this parasite...
  33. pmc Repeated domains of leptospira immunoglobulin-like proteins interact with elastin and tropoelastin
    Yi Pin Lin
    Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York, USA
    J Biol Chem 284:19380-91. 2009
    ..6-fold less than that of wild type LigBCon4. In summary, we show that Lig proteins of Leptospira spp. interact with elastin and HTE, and we conclude this interaction may contribute to Leptospira adhesion to host tissues during infection...
  34. pmc Clostridium difficile transcriptome analysis using pig ligated loop model reveals modulation of pathways not modulated in vitro
    Joy Scaria
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
    J Infect Dis 203:1613-20. 2011
    ..difficile pathways were uniquely expressed only in vivo. The pathways observed to be modulated only in this study could be targets of new therapeutic agents against C. difficile infection...
  35. pmc Cloning and molecular characterization of an immunogenic LigA protein of Leptospira interrogans
    Raghavan U M Palaniappan
    College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
    Infect Immun 70:5924-30. 2002
    ..LigA is the first leptospiral protein described to have 12 tandem repeats and is also the first to be expressed only during infection. Thus, LigA may have value in serodiagnosis or as a protective immunogen in novel vaccines...
  36. ncbi request reprint Development of a polymerase chain reaction test to confirm Mycobacterium avium subsp. paratuberculosis in culture
    Sung Jae Shin
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    J Vet Diagn Invest 16:116-20. 2004
    ..This PCR assay could detect 5-8 genome equivalents...
  37. pmc In vitro cellular immune responses to recombinant antigens of Mycobacterium avium subsp. paratuberculosis
    Sung Jae Shin
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Infect Immun 73:5074-85. 2005
    ..These data provide important insight into how improved vaccines against mycobacterial infections might be constructed...
  38. ncbi request reprint Cloning and characterization of putative zinc protease genes of Ehrlichia canis
    Ching Hao Teng
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    DNA Seq 14:109-21. 2003
    ..canis. ProA and ProB were both detectable in E. canis-infected DH82 cells. Sera from dogs, which were either naturally or experimentally infected with E. canis, recognized both the recombinant protein antigens...
  39. pmc Evaluation of a Mycobacterium avium subsp. paratuberculosis leuD mutant as a vaccine candidate against challenge in a caprine model
    Syed M Faisal
    Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY, USA
    Clin Vaccine Immunol 20:572-81. 2013
    ..avium subsp. paratuberculosis. In conclusion, the ΔleuD mutant is a promising vaccine candidate for development of a live attenuated vaccine for JD in ruminants...
  40. doi request reprint Evaluation of novel fusion proteins derived from extracellular matrix binding domains of LigB as vaccine candidates against leptospirosis in a hamster model
    Yongguo Cao
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, United States
    Vaccine 29:7379-86. 2011
    ..Our results demonstrate that this novel strategy could be promising in developing effective subunit vaccine to combat this zoonotic infection...
  41. pmc A novel fibronectin type III module binding motif identified on C-terminus of Leptospira immunoglobulin-like protein, LigB
    Yi Pin Lin
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Biochem Biophys Res Commun 389:57-62. 2009
    ..These data indicate that the LIPAD residues (LigB(1708-1712)) of the Leptospira interrogans LigB protein bind 15F(3) of Fn at a novel binding site, and this interaction contributes to adhesion to host cells...
  42. pmc Development of an enzyme-linked immunosorbent assay using a recombinant LigA fragment comprising repeat domains 4 to 7.5 as an antigen for diagnosis of equine leptospirosis
    WeiWei Yan
    Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA
    Clin Vaccine Immunol 20:1143-9. 2013
    ..5th repeat domain (LigACon4-7.5) as a diagnostic antigen for equine leptospirosis. This ELISA was found to be sensitive and specific, and it yielded results that concurred with those of the standard MAT. ..
  43. doi request reprint Immunostimulatory and antigen delivery properties of liposomes made up of total polar lipids from non-pathogenic bacteria leads to efficient induction of both innate and adaptive immune responses
    Syed M Faisal
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Vaccine 29:2381-91. 2011
    ..Taken together these results demonstrate the adjuvant potential of these novel lipid vesicles that may simultaneously induce both innate and adaptive immune responses due to their immune stimulatory and antigen delivery properties...
  44. ncbi request reprint A domain of the Leptospira LigB contributes to high affinity binding of fibronectin
    Yi Pin Lin
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Biochem Biophys Res Commun 362:443-8. 2007
    ..These results suggest that LigBCen2 contributed to high affinity binding on NTD or GBD of Fn, laminin, and fibrinogen and mediated Leptospira binding on host cells...
  45. ncbi request reprint Evaluation of lig-based conventional and real time PCR for the detection of pathogenic leptospires
    Raghavan U M Palaniappan
    College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Mol Cell Probes 19:111-7. 2005
    ..Hence, conventional and real time PCR with lig primers would be a sensitive and rapid tool for early diagnosis of leptospirosis...
  46. doi request reprint Immunogenicity and protective efficacy of recombinant Leptospira immunoglobulin-like protein B (rLigB) in a hamster challenge model
    WeiWei Yan
    College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Microbes Infect 11:230-7. 2009
    ..Overall, our results clearly demonstrate that LigB has emerged as novel protective antigen that can be used in future subunit vaccines against leptospirosis...
  47. doi request reprint Evaluation of factors associated with the risk of infection with Cryptosporidium parvum in dairy calves
    Barbara Szonyi
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Am J Vet Res 73:76-85. 2012
    ..To identify risk factors associated with Cryptosporidium parvum infection in dairy calves...
  48. pmc Microarray identification of Clostridium difficile core components and divergent regions associated with host origin
    Tavan Janvilisri
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    J Bacteriol 191:3881-91. 2009
    ..Due to a high degree of divergence among C. difficile strains, a core gene list from this study offers the first step toward the construction of diagnostic arrays for C. difficile...
  49. pmc Comparison of phenotypic and genotypic antimicrobial profiles in Escherichia coli and Salmonella enterica from the same dairy cattle farms
    Joy Scaria
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Mol Cell Probes 24:325-45. 2010
    ..coli isolated from different farms were closer than the profile of E. coli and Salmonella isolated from the same farm. This shows that the chance of multiple resistance gene transfers between these species is unlikely...
  50. ncbi request reprint Cloning and characterization of the Actinobacillus pleuropneumoniae fur gene and its role in regulation of ApxI and AfuABC expression
    Yuan Man Hsu
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
    DNA Seq 14:169-81. 2003
    ..This point mutation affected ApxI and Afu expression in response to iron, calcium, or Fur. These results provide further proof that calcium and the A. pleuropneumoniae Fur protein play a role in the expression of ApxI and Afu...
  51. pmc Cloning and characterization of an Ehrlichia canis gene encoding a protein localized to the morula membrane
    Ching Hao Teng
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Infect Immun 71:2218-25. 2003
    ..canis. Since the morula membrane is the interface between the ehrlichial growing environment and the host cytoplasm, MmpA may play a role in bacterium-host cell interactions...
  52. doi request reprint Evaluation of immune responses and protective efficacy in a goat model following immunization with a coctail of recombinant antigens and a polyprotein of Mycobacterium avium subsp. paratuberculosis
    Kumanan Kathaperumal
    Animal Health Diagnostic Center, Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, UpTwoer Road, Ithaca, NY 14853, USA
    Vaccine 27:123-35. 2009
    ..This study indicates that our multicomponent subunit vaccine induced a good Th1 response and conferred protection against MAP infection in a goat challenge model...
  53. pmc Manganese binds to Clostridium difficile Fbp68 and is essential for fibronectin binding
    Yi Pin Lin
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
    J Biol Chem 286:3957-69. 2011
    ..These results raise the possibility that Fbp68 plays a key role in C. difficile adherence on host cells to initiate infection...
  54. pmc Fibronectin binds to and induces conformational change in a disordered region of leptospiral immunoglobulin-like protein B
    Yi Pin Lin
    Department of Population Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    J Biol Chem 284:23547-57. 2009
    ..Upon binding to NTD, LigBCen2NR was observed by CD to adopt a beta-strand-rich structure, suggestive of the known beta-zipper mode of NTD binding...
  55. doi request reprint Proteomic comparison of historic and recently emerged hypervirulent Clostridium difficile strains
    Jenn Wei Chen
    Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York 14853, United States
    J Proteome Res 12:1151-61. 2013
    ..This work represents the first comparative proteomic analysis of historic and recently emerged hypervirulent C. difficile strains, complementing the previously published proteomics studies utilizing only one reference strain...
  56. pmc Transcriptional profiling of Clostridium difficile and Caco-2 cells during infection
    Tavan Janvilisri
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA
    J Infect Dis 202:282-90. 2010
    ....
  57. pmc Novel attenuated Salmonella enterica serovar Choleraesuis strains as live vaccine candidates generated by signature-tagged mutagenesis
    Yu We Ku
    College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
    Infect Immun 73:8194-203. 2005
    ..STM-attenuated live vaccine strains SC4A9 (gifsy-1) and SC2D2 (ssaV) were superior to commercially available live vaccine because they provided both safety and a protective immune response against challenge in pigs...
  58. pmc Differentiation of Escherichia coli pathotypes by oligonucleotide spotted array
    Raghavan U M Palaniappan
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    J Clin Microbiol 44:1495-501. 2006
    ..coli pathotypes and could also have a significant impact on the epidemiological analysis of E. coli infections...
  59. doi request reprint Calcium binds to leptospiral immunoglobulin-like protein, LigB, and modulates fibronectin binding
    Yi Pin Lin
    College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    J Biol Chem 283:25140-9. 2008
    ..We demonstrate that LigB is a novel bacterial Ca(2+)-binding protein and suggest that Ca(2+) binding plays a pivotal role in the pathogenesis of leptospirosis...
  60. ncbi request reprint Cloning and characterization of the genes coding for antigen 85A, 85B and 85C of Mycobacterium avium subsp. paratuberculosis
    Veerabadran Dheenadhayalan
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    DNA Seq 13:287-94. 2002
    ..However, the proposed three conserved regions involved in fibronectin binding in other mycobacteria were observed in N-terminal regions 85A, B and C of M. avium subsp. paratuberculosis...
  61. doi request reprint Immune response and protective efficacy of live attenuated Salmonella vaccine expressing antigens of Mycobacterium avium subsp. paratuberculosis against challenge in mice
    Subhash Chandra
    Department of Population Medicine and Diagnostic Sciences, Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Vaccine 31:242-51. 2012
    ..Thus, priming with a live recombinant Salmonella strain that secretes MAP antigens represents a promising approach that could lead to development of an efficacious and cost effective vaccine for Johne's disease...
  62. pmc Elastin, a novel extracellular matrix protein adhering to mycobacterial antigen 85 complex
    Chih Jung Kuo
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
    J Biol Chem 288:3886-96. 2013
    ..Moreover, binding of Ag85 on elastin siRNA-transfected Caco-2 cells was significantly reduced (34.3%), implying that elastin acts as an important ligand contributing to mycobacterial invasion...
  63. pmc Development of a microarray for identification of pathogenic Clostridium spp
    Tavan Janvilisri
    Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY 14853, USA
    Diagn Microbiol Infect Dis 66:140-7. 2010
    ..and provides advantages over traditional methods. Microarray-based techniques are promising applications for clinical diagnosis and epidemiologic investigations...
  64. ncbi request reprint Detection of mutations in the gyrA gene and class I integron from quinolone-resistant Salmonella enterica serovar Choleraesuis isolates in Taiwan
    Tzu Ming Huang
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14850, USA
    Vet Microbiol 100:247-54. 2004
    ..A surveillance system may be applied to the swine industry to monitor the emergence of fluoroquinolone and/or multi-drug-resistant S. enterica serovar Choleraesuis in Taiwan...
  65. ncbi request reprint Cloning and characterization of a Helicobacter bizzozeronii urease gene cluster
    Jiaqian Zhu
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    DNA Seq 13:321-31. 2002
    ..heilmanii, H. felis, H. pylori, H. mustelae and H. hepaticus. The UreI, E, F, G and H also showed 44-86% identity to that of H. pylori. Among these accessory genes, UreE had a lowest percentage identity to that of H. pylori...
  66. doi request reprint Prevalence of Giardia duodenalis assemblages among dairy herds in the New York City Watershed
    Miguella P Mark-Carew
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Vet Parasitol 185:151-7. 2012
    ..Interestingly, 100% of specimens identified as assemblage A were from calves under 84 days of age indicating that younger cattle are important reservoirs for potentially zoonotic assemblages of G. duodenalis...
  67. pmc Novel mycobacteria antigen 85 complex binding motif on fibronectin
    Chih Jung Kuo
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
    J Biol Chem 287:1892-902. 2012
    ..Ag85 might serve as an important colonization factor potentially contributing to mycobacterial virulence...
  68. doi request reprint Leptospira immunoglobulin-like protein B (LigB) binding to the C-terminal fibrinogen αC domain inhibits fibrin clot formation, platelet adhesion and aggregation
    Yi Pin Lin
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, NY, USA
    Mol Microbiol 79:1063-76. 2011
    ....
  69. doi request reprint Diagnosis of Borrelia-associated uveitis in two horses
    Heather L Priest
    Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY 14853, USA
    Vet Ophthalmol 15:398-405. 2012
    ..burgdorferi and the failure of such tests to confirm active infection, a combination of cytologic assessment, antibody, and/or PCR testing of ocular fluids may be worthwhile if the clinical suspicion for Lyme uveitis is high...
  70. ncbi request reprint Leptospirosis: pathogenesis, immunity, and diagnosis
    Raghavan U M Palaniappan
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
    Curr Opin Infect Dis 20:284-92. 2007
    ..Completion of the genomic sequences of leptospires has facilitated advances in diagnosis and prevention of the disease, and yielded insight into its pathogenesis. This article reviews this research, emphasizing recent progress...
  71. ncbi request reprint Immune responses with DNA vaccines encoded different gene fragments of severe acute respiratory syndrome coronavirus in BALB/c mice
    Zhijun Wang
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA
    Biochem Biophys Res Commun 327:130-5. 2005
    ....
  72. doi request reprint Sequence analysis of infectious bronchitis virus isolates from the 1960s in the United States
    Shankar Mondal
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Arch Virol 158:497-503. 2013
    ....
  73. pmc Microarray for molecular typing of Salmonella enterica serovars
    Joy Scaria
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Mol Cell Probes 22:238-43. 2008
    ....
  74. ncbi request reprint Cryptosporidium spp. from small mammals in the New York City watershed
    Peter E Ziegler
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
    J Wildl Dis 43:586-96. 2007
    ..Adult animals were more likely to shed the zoonotic strains of Cryptosporidium spp. Animals captured in the fall and winter were more likely to be infected with C. parvum than those captured in spring and summer...
  75. pmc A biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples
    Vijayarani Kumanan
    Animal Health Diagnostic Center, Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA
    J Vet Sci 10:35-42. 2009
    ....
  76. pmc Prevalence and characterization of multidrug-resistant (type ACSSuT) Salmonella enterica serovar Typhimurium strains in isolates from four gosling farms and a hatchery farm
    Chang You Yu
    Department of Veterinary Medicine, National Chiayi University, Chiayi, Taiwan
    J Clin Microbiol 46:522-6. 2008
    ..In conclusion, geese were a reservoir of diverse multidrug-resistant (type ACSSuT) S. enterica serovar Typhimurium strains, and each farm was colonized with genetically closely related S. enterica serovar Typhimurium strains...
  77. ncbi request reprint Omp52 is a growth-phase-regulated outer membrane protein of Leptospira santarosai serovar Shermani
    Wang Ju Hsieh
    Graduate Institute of Veterinary Medicine, National Taiwan University, No 1, Sec 4, Roosevelt Road, Da an District, Taipei City 106, Taiwan
    FEMS Microbiol Lett 243:339-45. 2005
    ..By using immunoblotting analysis, we proved that Omp52 was expressed in human patients infected with leptospires. These observations suggest that Omp52 may play roles in the interaction of host cells and pathogens during infection...
  78. ncbi request reprint Validation of an in-clinic enzyme-linked immunosorbent assay kit for diagnosis of Borrelia burgdorferi infection in horses
    Amy L Johnson
    Department of Clinical Studies, University of Pennsylvania, New Bolton Center, 382 West Street Road, Kennett Square, PA 19348, USA
    J Vet Diagn Invest 20:321-4. 2008
    ..burgdorferi. Validation of this test provides equine practitioners with an inexpensive, in-clinic method to confirm infection, although its moderate sensitivity may result in a moderate chance of a false negative test...
  79. ncbi request reprint Recombinant single-chain canine interleukin 12 induces interferon gamma mRNA expression in peripheral blood mononuclear cells of dogs with visceral leishmaniasis
    Lenita Ramires dos Santos
    Centro de Pesquisas Goncalo Moniz, Fundacao Oswaldo Cruz, Rua Waldemar Falcão, No 121, Brotas, Salvador Bahia, CEP 40295 001, Brazil
    Vet Immunol Immunopathol 98:43-8. 2004
    ..In addition, after stimulation with sccaIL-12, there was no difference between interferon gamma mRNA expressions in peripheral blood mononuclear cells of dogs with visceral leishmaniasis and from normal healthy control animals...
  80. ncbi request reprint Antimicrobial susceptibility of Actinobacillus pleuropneumoniae, Escherichia coli, and Salmonella choleraesuis recovered from Taiwanese swine
    Chao Fu Chang
    Department of Veterinary Medicine, National Taiwan University, Taipei
    J Vet Diagn Invest 14:153-7. 2002
    ..cholearesuis; chloramphenicol, lincomycin, and tetracycline were active only with A. pleuropneumoniae; neomycin was moderately active against A. pleuropneumoniae and E. coli. The other antimicrobials tested were inactive...
  81. ncbi request reprint Antimicrobial susceptibility of Riemerella anatipestifer isolated from ducks and the efficacy of ceftiofur treatment
    Chao Fu Chang
    Department of Veterinary Medicine, National Taiwan University, 142 ZhouShan Road, Taipei 106, Taiwan
    J Vet Diagn Invest 15:26-9. 2003
    ..anatipestifer. Ceftiofur (0, 0.25, 0.5, 1, and 2 mg/kg) was injected subcutaneously 5 hours after infection. A single dose of 2 mg/kg resulted in 73% survival as compared with 10% survival in the infected, but untreated controls...
  82. doi request reprint Quantification of SMN1 and SMN2 genes by capillary electrophoresis for diagnosis of spinal muscular atrophy
    Chun Chi Wang
    Faculty of Pharmacy, College of Pharmacy, Kaohsiung Medical University, and Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
    Electrophoresis 29:2904-11. 2008
    ..Our method showed good coincidence with them, and proved the misdiagnosis of DHPLC. This simple and reliable CE method is a powerful tool for clinical genotyping of large populations to detect carriers and SMA patients...
  83. ncbi request reprint Cloning and characterization of an iron regulated locus, iroA, in Salmonella enterica serovar Choleraesuis
    Wen sheng Wu
    Department of Veterinary Medicine, National Taiwan University, 142 ZhouShan Road, Taipei 106, Taiwan
    DNA Seq 13:333-41. 2002
    ..enterica serovar Choleraesuis by allelic exchange resulted in the loss of expression of the 78 kDa protein. However, this mutant had a similar LD50 to mice compared to the parent strain when given intraperitoneally...