S J Bedford-Guaus
Affiliation: Cornell University
- Microinjection of mouse phospholipase C zeta complementary RNA into mare oocytes induces long-lasting intracellular calcium oscillations and embryonic developmentSylvia J Bedford-Guaus
Department of Clinical Sciences, Cornell University, College of Veterinary Medicine, Ithaca, NY 14853, USA
Reprod Fertil Dev 20:875-83. 2008....
- Molecular cloning and characterization of phospholipase C zeta in equine sperm and testis reveals species-specific differences in expression of catalytically active proteinS J Bedford-Guaus
Departments of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA
Biol Reprod 85:78-88. 2011..Moreover, some of these results may suggest potential novel roles for PLCZ in sperm physiology...
- A defined medium supports changes consistent with capacitation in stallion sperm, as evidenced by increases in protein tyrosine phosphorylation and high rates of acrosomal exocytosisL A McPartlin
Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, United States
Theriogenology 69:639-50. 2008..The continuation of these studies might help to elucidate the conditions and pathways supporting sperm capacitation in the horse...
- Guanine-nucleotide exchange factors (RAPGEF3/RAPGEF4) induce sperm membrane depolarization and acrosomal exocytosis in capacitated stallion spermL A McPartlin
Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA
Biol Reprod 85:179-88. 2011..Altogether, these experiments support the hypothesis that RAPGEF3/RAPGEF4 activation regulates acrosomal exocytosis via its modulation of E(m), a novel role for RAPGEF3/RAPGEF4 in the series of events required to achieve fertilization...
- Hyperactivation of stallion sperm is required for successful in vitro fertilization of equine oocytesL A McPartlin
Departments of Clinical Sciences and Biological and Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
Biol Reprod 81:199-206. 2009..Conversely, sperm incubated in capacitating conditions but not treated with procaine did not fertilize (0%). These results support the hypothesis that capacitation and hyperactivation are required for successful IVF in the equine...