R L Houghton

Summary

Affiliation: Corixa Corporation
Country: USA

Publications

  1. ncbi request reprint A cloned antigen (recombinant K39) of Leishmania chagasi diagnostic for visceral leishmaniasis in human immunodeficiency virus type 1 patients and a prognostic indicator for monitoring patients undergoing drug therapy
    R L Houghton
    Corixa Corp, Seattle, Washington 98104, USA
    J Infect Dis 177:1339-44. 1998
  2. ncbi request reprint Identification of Babesia microti-specific immunodominant epitopes and development of a peptide EIA for detection of antibodies in serum
    Raymond L Houghton
    Department of Antigen Discovery, Corixa Corporation, Seattle, Washington 98104, USA
    Transfusion 42:1488-96. 2002
  3. pmc Use of multiepitope polyproteins in serodiagnosis of active tuberculosis
    Raymond L Houghton
    Corixa Corporation, Seattle, Washington 98104, USA
    Clin Diagn Lab Immunol 9:883-91. 2002
  4. ncbi request reprint Transcriptional complementarity in breast cancer: application to detection of circulating tumor cells
    R L Houghton
    Corixa Corporation, 1124 Columbia St, Ste 200, Seattle, WA 98104, USA
    Mol Diagn 6:79-91. 2001
  5. ncbi request reprint A multi-epitope synthetic peptide and recombinant protein for the detection of antibodies to Trypanosoma cruzi in radioimmunoprecipitation-confirmed and consensus-positive sera
    R L Houghton
    Corixa Corporation, Seattle, WA 98104, USA
    J Infect Dis 179:1226-34. 1999
  6. ncbi request reprint Multiepitope synthetic peptide and recombinant protein for the detection of antibodies to Trypanosoma cruzi in patients with treated or untreated Chagas' disease
    R L Houghton
    Corixa Corporation, Seattle, WA 98104
    J Infect Dis 181:325-30. 2000
  7. pmc Serological expression cloning and immunological evaluation of MTB48, a novel Mycobacterium tuberculosis antigen
    M J Lodes
    Corixa Corporation, Seattle, Washington 98104, USA
    J Clin Microbiol 39:2485-93. 2001
  8. pmc Mass spectrometric identification of mtb81, a novel serological marker for tuberculosis
    R C Hendrickson
    Corixa Corporation, Seattle Washington 98140, USA
    J Clin Microbiol 38:2354-61. 2000
  9. pmc Serological expression cloning of novel immunoreactive antigens of Babesia microti
    M J Lodes
    Corixa Corporation, Seattle, Washington 98104, USA
    Infect Immun 68:2783-90. 2000
  10. ncbi request reprint Detection of mammaglobin in the sera of patients with breast cancer
    G R Fanger
    Corixa Corporation, Department of Antigen Discovery, Seattle, WA 98104, USA
    Tumour Biol 23:212-21. 2002

Detail Information

Publications26

  1. ncbi request reprint A cloned antigen (recombinant K39) of Leishmania chagasi diagnostic for visceral leishmaniasis in human immunodeficiency virus type 1 patients and a prognostic indicator for monitoring patients undergoing drug therapy
    R L Houghton
    Corixa Corp, Seattle, Washington 98104, USA
    J Infect Dis 177:1339-44. 1998
    ..In contrast, most patients who clinically relapsed showed increased antibody titers to rK39. These data demonstrate the diagnostic and prognostic utility of rK39 in detecting active visceral leishmaniasis...
  2. ncbi request reprint Identification of Babesia microti-specific immunodominant epitopes and development of a peptide EIA for detection of antibodies in serum
    Raymond L Houghton
    Department of Antigen Discovery, Corixa Corporation, Seattle, Washington 98104, USA
    Transfusion 42:1488-96. 2002
    ..To develop a test that can detect antibody responses to B. microti, peptide epitopes identified in two serocomplementary B. microti-specific antigens were used in a prototype EIA...
  3. pmc Use of multiepitope polyproteins in serodiagnosis of active tuberculosis
    Raymond L Houghton
    Corixa Corporation, Seattle, Washington 98104, USA
    Clin Diagn Lab Immunol 9:883-91. 2002
    ..The data indicate that the use of multiple antigens, some of which are in a single polyprotein, can be used to facilitate the development of a highly sensitive test for M. tuberculosis antibody detection...
  4. ncbi request reprint Transcriptional complementarity in breast cancer: application to detection of circulating tumor cells
    R L Houghton
    Corixa Corporation, 1124 Columbia St, Ste 200, Seattle, WA 98104, USA
    Mol Diagn 6:79-91. 2001
    ..We used a combination of genetic subtraction, silicon DNA microarray analysis, and quantitative PCR to identify tissue- and tumor-specific genes as diagnostic targets for breast cancer...
  5. ncbi request reprint A multi-epitope synthetic peptide and recombinant protein for the detection of antibodies to Trypanosoma cruzi in radioimmunoprecipitation-confirmed and consensus-positive sera
    R L Houghton
    Corixa Corporation, Seattle, WA 98104, USA
    J Infect Dis 179:1226-34. 1999
    ..These studies show that peptides and recombinants containing multiple repeat epitopes are powerful tools for developing assays for T. cruzi antibody detection and have direct application in blood screening...
  6. ncbi request reprint Multiepitope synthetic peptide and recombinant protein for the detection of antibodies to Trypanosoma cruzi in patients with treated or untreated Chagas' disease
    R L Houghton
    Corixa Corporation, Seattle, WA 98104
    J Infect Dis 181:325-30. 2000
    ..The results indicate that multiepitope antigens provide a more sensitive and specific alternative to lysate for detection of anti-T. cruzi antibodies, as required for developing blood screening assays...
  7. pmc Serological expression cloning and immunological evaluation of MTB48, a novel Mycobacterium tuberculosis antigen
    M J Lodes
    Corixa Corporation, Seattle, Washington 98104, USA
    J Clin Microbiol 39:2485-93. 2001
    ..tuberculosis infection when it is combined with other known immunodominant antigens, such as the 38-kDa antigen...
  8. pmc Mass spectrometric identification of mtb81, a novel serological marker for tuberculosis
    R C Hendrickson
    Corixa Corporation, Seattle Washington 98140, USA
    J Clin Microbiol 38:2354-61. 2000
    ..Together, these results demonstrate that Mtb81 may be a promising complementary antigen for the serodiagnosis of TB...
  9. pmc Serological expression cloning of novel immunoreactive antigens of Babesia microti
    M J Lodes
    Corixa Corporation, Seattle, Washington 98104, USA
    Infect Immun 68:2783-90. 2000
    ..Western blot analysis with the resulting recombinant proteins indicated that these antigens were targets of humoral immune responses during B. microti infection in humans...
  10. ncbi request reprint Detection of mammaglobin in the sera of patients with breast cancer
    G R Fanger
    Corixa Corporation, Department of Antigen Discovery, Seattle, WA 98104, USA
    Tumour Biol 23:212-21. 2002
    ..Thus, mammaglobin, which is overexpressed and secreted from breast carcinoma cells, is detectable in sera of patients with breast cancer and may provide a rapid screening test for the diagnosis and management of breast cancer...
  11. pmc Serodiagnosis of human granulocytic ehrlichiosis by using novel combinations of immunoreactive recombinant proteins
    M J Lodes
    Corixa Corporation, 1124 Columbia St, Suite 200, Seattle, WA 98104, USA
    J Clin Microbiol 39:2466-76. 2001
    ..2% (23 of 27) of acute-phase patient serum samples are detected, indicating the potential of these antigens for use in the development of a rapid serologic assay for the detection of E. phagocytophila infection...
  12. ncbi request reprint WT1-specific serum antibodies in patients with leukemia
    A Gaiger
    Corixa Corporation, Seattle, Washington 98104, USA
    Clin Cancer Res 7:761s-765s. 2001
    ..These data provide further stimulus to test therapeutic vaccines directed against WT1 with increased expectation that the vaccines will be able to elicit and/or boost an immune response to WT1...
  13. ncbi request reprint Identification and characterization of prostein, a novel prostate-specific protein
    J Xu
    Corixa Corp, Seattle Washington 98104, USA
    Cancer Res 61:1563-8. 2001
    ..These results validate prostein as a prostate-specific marker with potential utility in the diagnosis and treatment of prostate cancer...
  14. ncbi request reprint Demonstrable parasitemia among Connecticut blood donors with antibodies to Babesia microti
    David A Leiby
    Department of Transmissible Diseases, American Red Cross, Rockville, MD 20855, USA
    Transfusion 45:1804-10. 2005
    ..Although several studies have measured B. microti antibodies in blood donors, little is known about associated parasitemia and the inherent risk of transmitting the parasite by transfusion...
  15. ncbi request reprint P704P, P712P, and P775P: A genomic cluster of prostate-specific genes
    John A Stolk
    Corixa Corporation, Seattle, Washington 98104, USA
    Prostate 60:214-26. 2004
    ..Such understanding will likely result in the development of promising new markers for screening, diagnosis, and prognosis, as well as potential therapeutic approaches for combating this disease...
  16. ncbi request reprint Expression cloning
    Michael J Lodes
    Corixa Corporation, Seattle, WA, USA
    Methods Mol Med 94:91-106. 2004
    ..or E. phagocytophila), Babesia microti, Trypanosoma cruzi, Leishmania chagasi, and Chlamydia spp...
  17. ncbi request reprint Serum antibodies to lipophilin B detected in late stage breast cancer patients
    Darrick Carter
    Department of Tumor Antigen Discovery, Corixa Corporation, Seattle, Washington 98104, USA
    Clin Cancer Res 9:749-54. 2003
    ..A polymorphism identified in Lipophilin B did not appear to influence human sera reactivity. The data indicate that humoral immune responses to lipophilin B may serve as a diagnostic indicator, particularly for breast cancer...
  18. pmc Mammaglobin as a novel breast cancer biomarker: multigene reverse transcription-PCR assay and sandwich ELISA
    Barbara K Zehentner
    Corixa Corporation, Seattle, WA 98101, USA
    Clin Chem 50:2069-76. 2004
    ..The aim of this study was to examine the potential usefulness of a mammaglobin multigene reverse transcription-PCR (RT-PCR) assay and a mammaglobin sandwich ELISA as diagnostic tools in breast cancer...
  19. pmc Identification and characterization of putative secreted antigens from Babesia microti
    Mary J Homer
    Corixa Corporation, Seattle, Washington 98104, USA
    J Clin Microbiol 41:723-9. 2003
    ..Initial serological data obtained with recombinant proteins and a patient serum panel demonstrated that several of the proteins could be useful in developing diagnostic tests for detection of B. microti antibodies and antigens in serum...
  20. ncbi request reprint Relationship between tick bites and the seroprevalence of Babesia microti and Anaplasma phagocytophila (previously Ehrlichia sp.) in blood donors
    David A Leiby
    Transmissible Diseases Department, American Red Cross, Rockville, MD 20855, USA
    Transfusion 42:1585-91. 2002
    ..Despite an increased recognition of the threat tick-borne agents pose to blood safety, our understanding of the prevalence and transmissibility of these agents in blood donors is limited...
  21. ncbi request reprint Characterization of KLK4 expression and detection of KLK4-specific antibody in prostate cancer patient sera
    Craig H Day
    Corixa Corporation, 1124 Columbia Street, Suite 200, Seattle, Washington, WA 98104, USA
    Oncogene 21:7114-20. 2002
    ....
  22. pmc Application of a multigene reverse transcription-PCR assay for detection of mammaglobin and complementary transcribed genes in breast cancer lymph nodes
    Barbara K Zehentner
    Corixa, 1124 Columbia St, Seattle, WA 98104, USA
    Clin Chem 48:1225-31. 2002
    ..Collectively the expression profile of these four genes could be used as a diagnostic and prognostic indicator for breast cancer...
  23. ncbi request reprint Purification and characterization of the mammaglobin/lipophilin B complex, a promising diagnostic marker for breast cancer
    Darrick Carter
    Department of Antigen Discovery, Corixa Corp, 1124 Columbia Street, Suite 200, Seattle, WA 98104, USA
    Biochemistry 41:6714-22. 2002
    ..This work describes the biochemistry of the mammaglobin/lipophilin B complex and lays the framework for use of this complex as a novel protein-based serological marker for breast cancer...
  24. ncbi request reprint Discovery of differentially expressed genes in human breast cancer using subtracted cDNA libraries and cDNA microarrays
    Yuqiu Jiang
    Corixa Corporation, 1124 Columbia Street, Suite 200, Seattle, Washington, WA 98104, USA
    Oncogene 21:2270-82. 2002
    ..These differentially expressed genes may be of potential utility as therapeutic and diagnostic targets for breast cancer...
  25. ncbi request reprint Detection of circulating tumor cells in peripheral blood of breast cancer patients during or after therapy using a multigene real-time RT-PCR assay
    Barbara K Zehentner
    HematoLogics, Inc, Seattle, Washington, USA
    Mol Diagn Ther 10:41-7. 2006
    ..To evaluate the utility of a multigene real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay to detect circulating tumor cells in peripheral blood specimens of breast cancer patients during or after treatment...
  26. ncbi request reprint Detection of alpha-methylacyl-coenzyme-A racemase transcripts in blood and urine samples of prostate cancer patients
    Barbara K Zehentner
    HematoLogics Inc, Seattle, Washington 98109, USA
    Mol Diagn Ther 10:397-403. 2006
    ..In addition, we have undertaken a pilot study to demonstrate the potential application of this technique for the detection of prostate tumor cells in urine samples from patients with prostate cancer...