Nan Wang

Summary

Affiliation: Columbia University
Country: USA

Publications

  1. ncbi Regulation and mechanisms of ATP-binding cassette transporter A1-mediated cellular cholesterol efflux
    Nan Wang
    Division of Molecular Medicine, Department of Medicine, Columbia University, 630 West 168 Street, New York, NY 10032, USA
    Arterioscler Thromb Vasc Biol 23:1178-84. 2003
  2. pmc A PEST sequence in ABCA1 regulates degradation by calpain protease and stabilization of ABCA1 by apoA-I
    Nan Wang
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, New York 10032, USA
    J Clin Invest 111:99-107. 2003
  3. pmc ATP-binding cassette transporters G1 and G4 mediate cellular cholesterol efflux to high-density lipoproteins
    Nan Wang
    Department of Medicine, Division of Molecular Medicine, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 101:9774-9. 2004
  4. ncbi ATP-binding cassette transporter A1 (ABCA1) functions as a cholesterol efflux regulatory protein
    N Wang
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, New York 10032, USA
    J Biol Chem 276:23742-7. 2001
  5. ncbi LXR-induced redistribution of ABCG1 to plasma membrane in macrophages enhances cholesterol mass efflux to HDL
    Nan Wang
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, NY 10032, USA
    Arterioscler Thromb Vasc Biol 26:1310-6. 2006
  6. ncbi Specific binding of ApoA-I, enhanced cholesterol efflux, and altered plasma membrane morphology in cells expressing ABC1
    N Wang
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, New York 10032, USA
    J Biol Chem 275:33053-8. 2000
  7. ncbi ATP-binding cassette transporters G1 and G4 mediate cholesterol and desmosterol efflux to HDL and regulate sterol accumulation in the brain
    Nan Wang
    Department of Medicine, Columbia University, PS 8 401, 630 W 168th St, New York, NY 10032, USA
    FASEB J 22:1073-82. 2008
  8. ncbi ATP-binding cassette transporter A7 (ABCA7) binds apolipoprotein A-I and mediates cellular phospholipid but not cholesterol efflux
    Nan Wang
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, New York 10032, USA
    J Biol Chem 278:42906-12. 2003
  9. pmc Increased inflammatory gene expression in ABC transporter-deficient macrophages: free cholesterol accumulation, increased signaling via toll-like receptors, and neutrophil infiltration of atherosclerotic lesions
    Laurent Yvan-Charvet
    Division of Molecular Medicine, Department of Medicine, Columbia University, 630 W 168th St, New York, NY 10032, USA
    Circulation 118:1837-47. 2008
  10. pmc Combined deficiency of ABCA1 and ABCG1 promotes foam cell accumulation and accelerates atherosclerosis in mice
    Laurent Yvan-Charvet
    Division of Molecular Medicine, Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    J Clin Invest 117:3900-8. 2007

Detail Information

Publications72

  1. ncbi Regulation and mechanisms of ATP-binding cassette transporter A1-mediated cellular cholesterol efflux
    Nan Wang
    Division of Molecular Medicine, Department of Medicine, Columbia University, 630 West 168 Street, New York, NY 10032, USA
    Arterioscler Thromb Vasc Biol 23:1178-84. 2003
    ..This and other evidence suggest that ABCA1 promotes cholesterol and phospholipid efflux, probably by directly transporting both lipids as substrates...
  2. pmc A PEST sequence in ABCA1 regulates degradation by calpain protease and stabilization of ABCA1 by apoA-I
    Nan Wang
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, New York 10032, USA
    J Clin Invest 111:99-107. 2003
    ..Inhibition of ABCA1 degradation by calpain could represent a novel therapeutic approach to increasing macrophage cholesterol efflux and decreasing atherosclerosis...
  3. pmc ATP-binding cassette transporters G1 and G4 mediate cellular cholesterol efflux to high-density lipoproteins
    Nan Wang
    Department of Medicine, Division of Molecular Medicine, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 101:9774-9. 2004
    ..ABCG1 is highly expressed in macrophages and probably mediates cholesterol efflux from macrophage foam cells to the major HDL fractions, providing a mechanism to explain the relationship between HDL levels and atherosclerosis risk...
  4. ncbi ATP-binding cassette transporter A1 (ABCA1) functions as a cholesterol efflux regulatory protein
    N Wang
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, New York 10032, USA
    J Biol Chem 276:23742-7. 2001
    ..The data suggest that the binding of apoA-I to ABCA1 leads to the formation of phospholipid-apoA-I complexes, which subsequently promote cholesterol efflux in an autocrine or paracrine fashion...
  5. ncbi LXR-induced redistribution of ABCG1 to plasma membrane in macrophages enhances cholesterol mass efflux to HDL
    Nan Wang
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, NY 10032, USA
    Arterioscler Thromb Vasc Biol 26:1310-6. 2006
    ..This study examines the ABCG1-mediated cholesterol efflux and intracellular cholesterol transport by studying the ABCG1 localization and function in macrophages...
  6. ncbi Specific binding of ApoA-I, enhanced cholesterol efflux, and altered plasma membrane morphology in cells expressing ABC1
    N Wang
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, New York 10032, USA
    J Biol Chem 275:33053-8. 2000
    ..Plasma membrane ABC1 may act as a phospholipid/cholesterol flippase, providing lipid to bound apoA-I, or to the outer membrane hemileaflet...
  7. ncbi ATP-binding cassette transporters G1 and G4 mediate cholesterol and desmosterol efflux to HDL and regulate sterol accumulation in the brain
    Nan Wang
    Department of Medicine, Columbia University, PS 8 401, 630 W 168th St, New York, NY 10032, USA
    FASEB J 22:1073-82. 2008
    ....
  8. ncbi ATP-binding cassette transporter A7 (ABCA7) binds apolipoprotein A-I and mediates cellular phospholipid but not cholesterol efflux
    Nan Wang
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, New York 10032, USA
    J Biol Chem 278:42906-12. 2003
    ..These studies show that ABCA7 has the ability to bind apolipoproteins and promote efflux of cellular phospholipids without cholesterol, and they suggest a possible role of ABCA7 in cellular phospholipid metabolism in peripheral tissues...
  9. pmc Increased inflammatory gene expression in ABC transporter-deficient macrophages: free cholesterol accumulation, increased signaling via toll-like receptors, and neutrophil infiltration of atherosclerotic lesions
    Laurent Yvan-Charvet
    Division of Molecular Medicine, Department of Medicine, Columbia University, 630 W 168th St, New York, NY 10032, USA
    Circulation 118:1837-47. 2008
    ..This study was undertaken to elucidate the mechanisms and consequences of enhanced inflammatory gene expression in ABC transporter-deficient macrophages...
  10. pmc Combined deficiency of ABCA1 and ABCG1 promotes foam cell accumulation and accelerates atherosclerosis in mice
    Laurent Yvan-Charvet
    Division of Molecular Medicine, Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    J Clin Invest 117:3900-8. 2007
    ..These results suggest that the combined effects of ABCA1 and ABCG1 in mediating macrophage sterol efflux are central to the antiatherogenic properties of HDL...
  11. pmc ATP-binding cassette transporters and HDL suppress hematopoietic stem cell proliferation
    Laurent Yvan-Charvet
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, NY 10032, USA
    Science 328:1689-93. 2010
    ..The findings indicate that ABCA1, ABCG1, and HDL inhibit the proliferation of hematopoietic stem and multipotential progenitor cells and connect expansion of these populations with leukocytosis and accelerated atherosclerosis...
  12. ncbi SR-BI inhibits ABCG1-stimulated net cholesterol efflux from cells to plasma HDL
    Laurent Yvan-Charvet
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, NY, USA
    J Lipid Res 49:107-14. 2008
    ..Previous findings of increased atherosclerosis in mice transplanted with SR-BI(-/-) bone marrow probably cannot be explained by a defect in macrophage cholesterol efflux...
  13. pmc ABCG1 and HDL protect against endothelial dysfunction in mice fed a high-cholesterol diet
    Naoki Terasaka
    Division of Molecular Medicine, Columbia University College of Physicians and Surgeons, New York, New York, USA
    J Clin Invest 118:3701-13. 2008
    ..Our data suggest that ABCG1 and HDL maintain EC function in HCD-fed mice by promoting efflux of cholesterol and 7-oxysterols and preserving active eNOS dimer levels...
  14. ncbi A PEST deletion mutant of ABCA1 shows impaired internalization and defective cholesterol efflux from late endosomes
    Wengen Chen
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, NY 10032, USA
    J Biol Chem 280:29277-81. 2005
    ....
  15. ncbi Decreased atherosclerosis in low-density lipoprotein receptor knockout mice transplanted with Abcg1-/- bone marrow
    Mollie Ranalletta
    Division of Molecular Medicine, Department of Medicine, Columbia University, 630 West 168th St, New York, NY 10032, USA
    Arterioscler Thromb Vasc Biol 26:2308-15. 2006
    ..Recent studies indicate that the ATP-binding cassette transporter ABCG1 can promote cholesterol efflux from macrophages to high-density lipoprotein. This study was designed to assess the in vivo role of macrophage ABCG1 in atherosclerosis...
  16. pmc HDL from CETP-deficient subjects shows enhanced ability to promote cholesterol efflux from macrophages in an apoE- and ABCG1-dependent pathway
    Fumihiko Matsuura
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, New York, USA
    J Clin Invest 116:1435-42. 2006
    ..Thus, CETP-D HDL has enhanced ability to promote cholesterol efflux from foam cells in an ABCG1-dependent pathway due to an increased content of LCAT and apoE...
  17. ncbi Potential role of ABCA7 in cellular lipid efflux to apoA-I
    Patrick Linsel-Nitschke
    Division of Molecular Medicine, College of Physicians and Surgeons, Columbia University, New York, NY, USA
    J Lipid Res 46:86-92. 2005
    ..Although ABCA7 does not contribute to apolipoprotein-mediated lipid efflux in resting macrophages, its cell surface location in the kidney suggests that it could serve such a role in tissue microenvironments...
  18. pmc High-density lipoprotein protects macrophages from oxidized low-density lipoprotein-induced apoptosis by promoting efflux of 7-ketocholesterol via ABCG1
    Naoki Terasaka
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 104:15093-8. 2007
    ..These findings indicate a specific role for ABCG1 in promoting efflux of 7-ketocholesterol and related oxysterols from macrophages onto HDL and in protecting these cells from oxysterol-induced cytotoxicity...
  19. pmc Deficiency of ATP-binding cassette transporters A1 and G1 in macrophages increases inflammation and accelerates atherosclerosis in mice
    Marit Westerterp
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, NY 10032, USA
    Circ Res 112:1456-65. 2013
    ..Although it is widely assumed that this is attributable to the ability of high-density lipoprotein to promote cholesterol efflux from macrophage foam cells, direct experimental support for this hypothesis is lacking...
  20. pmc Reduced VLDL clearance in Apoe(-/-)Npc1(-/-) mice is associated with increased Pcsk9 and Idol expression and decreased hepatic LDL-receptor levels
    Minako Ishibashi
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, NY, USA
    J Lipid Res 51:2655-63. 2010
    ..In contrast to modest induction of hepatic IDOL with synthetic LXR ligands, a striking upregulation of IDOL in Apoe(-/-)Npc1(-/-) mice could indicate a role of endogenous LXR ligands in regulation of hepatic IDOL...
  21. ncbi Identification of small PDZK1-associated protein, DD96/MAP17, as a regulator of PDZK1 and plasma high density lipoprotein levels
    David L Silver
    Department of Medicine, Division of Molecular Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    J Biol Chem 278:28528-32. 2003
    ..Thus, SPAP may be an endogenous regulator of cellular PDZK1 levels by regulating PDZK1 turnover...
  22. pmc ATP-binding cassette transporter A7 enhances phagocytosis of apoptotic cells and associated ERK signaling in macrophages
    Andreas W Jehle
    Department of Medicine, Columbia University, New York, NY 10032, USA
    J Cell Biol 174:547-56. 2006
    ..These studies reveal a major role of ABCA7 and not -A1 in the clearance of apoptotic cells and therefore suggest that ABCA7 is an authentic orthologue of CED-7...
  23. ncbi Deficiency of ATP-binding cassette transporter B6 in megakaryocyte progenitors accelerates atherosclerosis in mice
    Andrew J Murphy
    From Haematopoiesis and Leukocyte Biology, Baker IDI Heart and Diabetes Institute, Melbourne, Australia A J M Department of Immunology, Monash University, Melbourne, Australia A J M Institut National de la Sante et de la Recherche Medicale U1065, Centre Mediterraneen de Medecine Molecuaire C3M, ATIP AVENIR, Nice, France V S, L Y C Department of Medicine, Division of Molecular Medicine, Columbia University, New York, NY N W, N B, S A, M W, C B W and Department of Pharmaceutical Sciences, St Jude Children s Research Hospital, Memphis, TN J D S
    Arterioscler Thromb Vasc Biol 34:751-8. 2014
    ..The ATP-binding cassette (ABC) transporter B6 (ABCB6) is highly expressed in megakaryocyte progenitors, but its role in platelet production and disease has not been elucidated...
  24. ncbi Phosphorylation of a pest sequence in ABCA1 promotes calpain degradation and is reversed by ApoA-I
    Laurent O Martinez
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, New York 10032, USA
    J Biol Chem 278:37368-74. 2003
    ....
  25. ncbi Inhibition of cholesteryl ester transfer protein by torcetrapib modestly increases macrophage cholesterol efflux to HDL
    Laurent Yvan-Charvet
    Division of Molecular Medicine, Department of Medicine, Columbia University, 630 West 168 St, New York, NY 10032, USA
    Arterioscler Thromb Vasc Biol 27:1132-8. 2007
    ..This study examines the effects of pharmacological inhibition of cholesteryl ester transfer protein (CETP) on the ability of high-density lipoprotein particles (HDL) to promote net cholesterol efflux from human THP-1 macrophage foam cells...
  26. ncbi PU.1 and a TTTAAA element in the myeloid defensin-1 promoter create an operational TATA box that can impose cell specificity onto TFIID function
    Mariana Yaneva
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, and Weill Graduate School of Medical Sciences, Cornell University, New York, NY 10021, USA
    J Immunol 176:6906-17. 2006
    ..As GABP is widely expressed, a strong TATA box thus alleviates promyelocytic cell specificity of the def1 promoter. These findings suggest how the myeloid def1 promoter may have evolutionarily acquired its current properties...
  27. doi ATP-binding cassette transporter G1 and high-density lipoprotein promote endothelial NO synthesis through a decrease in the interaction of caveolin-1 and endothelial NO synthase
    Naoki Terasaka
    Division of Molecular Medicine, Department of Medicine, Columbia University, 630 W 168 St, New York, NY 10032, USA
    Arterioscler Thromb Vasc Biol 30:2219-25. 2010
    ..To investigate whether cholesterol efflux to high-density lipoprotein (HDL) via ATP-binding cassette transporter G1 (ABCG1) modulates the interaction of caveolin (Cav) 1 and endothelial NO synthase (eNOS)...
  28. pmc Regulation and mechanisms of macrophage cholesterol efflux
    Alan R Tall
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, New York 10032, USA
    J Clin Invest 110:899-904. 2002
  29. ncbi Pivotal advance: macrophages become resistant to cholesterol-induced death after phagocytosis of apoptotic cells
    Dongying Cui
    Department of Medicine, Columbia University, New York, NY 10032, USA
    J Leukoc Biol 82:1040-50. 2007
    ..These findings have implications for macrophage physiology in both AC clearance and atherosclerotic plaque progression...
  30. pmc Pegylation of high-density lipoprotein decreases plasma clearance and enhances antiatherogenic activity
    Andrew J Murphy
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, NY 10032, USA
    Circ Res 113:e1-9. 2013
    ..However, rapid clearance leads to a requirement for repeated administration of large amounts of material and limits effective plasma concentrations...
  31. pmc Role of HDL, ABCA1, and ABCG1 transporters in cholesterol efflux and immune responses
    Laurent Yvan-Charvet
    Division of Molecular Medicine, Department of Medicine, Columbia University, 630 W 168th St, New York, NY 10032, USA
    Arterioscler Thromb Vasc Biol 30:139-43. 2010
    ..The underlying mechanisms may involve modulation of sterol levels and lipid organization in cell membranes...
  32. ncbi Scavenger receptor BI (SR-BI) is up-regulated in adrenal gland in apolipoprotein A-I and hepatic lipase knock-out mice as a response to depletion of cholesterol stores. In vivo evidence that SR-BI is a functional high density lipoprotein receptor under fe
    N Wang
    Department of Medicine, Columbia University, New York, New York 10032, USA
    J Biol Chem 271:21001-4. 1996
    ..These findings provide in vivo evidence showing SR-BI is a functional HDL receptor under feedback control. The action of hepatic lipase on apoA-I-containing lipoproteins may facilitate the SR-BI-mediated uptake of HDL lipid...
  33. pmc Cholesterol efflux in megakaryocyte progenitors suppresses platelet production and thrombocytosis
    Andrew J Murphy
    Department of Medicine, Division of Molecular Medicine, Columbia University, New York, New York, USA
    Nat Med 19:586-94. 2013
    ..HDL infusions may offer a new approach to reducing atherothrombotic events associated with increased platelet production...
  34. doi HDL, ABC transporters, and cholesterol efflux: implications for the treatment of atherosclerosis
    Alan R Tall
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, NY 10032, USA
    Cell Metab 7:365-75. 2008
    ..A variety of approaches to increasing HDL may eventually be successful in treating atherosclerosis...
  35. doi Panax notoginseng reduces atherosclerotic lesions in ApoE-deficient mice and inhibits TNF-alpha-induced endothelial adhesion molecule expression and monocyte adhesion
    Jian Bo Wan
    Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    J Agric Food Chem 57:6692-7. 2009
    ..In conclusion, PNS has antiatherogenic activity through, at least in part, its lipid-lowering and antivascular inflammatory mechanisms...
  36. ncbi ATP-binding cassette transporters, atherosclerosis, and inflammation
    Marit Westerterp
    From the Division of Molecular Medicine, Department of Medicine, Columbia University, New York, NY M W, A E B, L Y C, A J M, N W, A R T Departments of Medical Biochemistry M W and Vascular Medicine A E B, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands and Haematopoiesis and Leukocyte Biology, Baker IDI Heart and Diabetes Institute, Melbourne, Australia A J M
    Circ Res 114:157-70. 2014
    ....
  37. ncbi Hepatic scavenger receptor BI promotes rapid clearance of high density lipoprotein free cholesterol and its transport into bile
    Y Ji
    Division of Molecular Medicine, Department of Medicine, Columbia University, New York, New York 10032, USA
    J Biol Chem 274:33398-402. 1999
    ..Thus, in the mouse, a major portion of the clearance of HDL FC from plasma is mediated by SR-BI...
  38. doi β-adrenergic receptor blockade reduces endoplasmic reticulum stress and normalizes calcium handling in a coronary embolization model of heart failure in canines
    Isaac George
    Division of Cardiothoracic Surgery, College of Physicians and Surgeons of Columbia University, New York, NY, USA
    Cardiovasc Res 91:447-55. 2011
    ..However, the role of ER stress in β-blocker therapy for congestive heart failure (CHF) has not been studied. This study examined ER stress in CHF and evaluated its role in β-blocker therapy in a canine model of ischaemic CHF...
  39. pmc Defective HDL particle uptake in ob/ob hepatocytes causes decreased recycling, degradation, and selective lipid uptake
    D L Silver
    The Division of Molecular Medicine, Department of Medicine, Columbia University, New York, New York 10032, USA
    J Clin Invest 105:151-9. 2000
    ..The findings show a major defect in HDL uptake and recycling in ob/ob hepatocytes and suggest that HDL recycling through the ERC plays a role in the determination of plasma HDL protein and cholesterol levels...
  40. ncbi High density lipoprotein (HDL) particle uptake mediated by scavenger receptor class B type 1 results in selective sorting of HDL cholesterol from protein and polarized cholesterol secretion
    D L Silver
    Department of Medicine, Division of Molecular Medicine, Columbia University, New York, NY 10032, USA
    J Biol Chem 276:25287-93. 2001
    ..A distinct process not mediated by SR-BI is involved in uptake and degradation of apoE-free HDL in hepatocytes...
  41. ncbi Delayed-late activation of a myeloid defensin minimal promoter by retinoids and inflammatory mediators
    Nan Wang
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Leuk Res 28:879-89. 2004
    ..We propose that D1BP and PU.1 are the end-points of separate pathways...
  42. ncbi The roles of different pathways in the release of cholesterol from macrophages
    Maria Pia Adorni
    Gastroenterology, Hepatology, and Nutrition Division, Children s Hospital of Philadelphia, Philadelphia, PA, USA
    J Lipid Res 48:2453-62. 2007
    ..Another pathway that is present in all cells is aqueous diffusion. Our studies demonstrate that this mechanism is one of the major contributors to efflux, particularly in cholesterol-normal cells...
  43. ncbi The cholesterol transporter ABCG1 modulates the subcellular distribution and proteolytic processing of beta-amyloid precursor protein
    Gavin H Tansley
    Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada
    J Lipid Res 48:1022-34. 2007
    ..These findings suggest that altered cholesterol metabolism and APP trafficking mediated by ABCG1 may contribute to the accelerated onset of AD neuropathology in DS...
  44. ncbi Down-regulation of gut-enriched Kruppel-like factor expression in esophageal cancer
    Nan Wang
    National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences, Beijing 100021, China
    World J Gastroenterol 8:966-70. 2002
    ..It is of interest to study the expression and role of GKLF in esophageal carcinoma...
  45. ncbi [Effect of expression of gut-enriched Kruppel-like factor on esophageal squamous cancer cells]
    Nan Wang
    National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences, Beijing 100021, China
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 35:55-8. 2003
    ....
  46. ncbi Characterization of human tear proteome using multiple proteomic analysis techniques
    Nan Li
    Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
    J Proteome Res 4:2052-61. 2005
    ..This work represents one of the most extensive proteome profiles (i.e., proteins identified and PTMs characterized) generated from tear fluids using clinically relevant amounts of sample...
  47. ncbi Construction, gene delivery, and expression of DNA tethered nanoparticles
    Tarl Prow
    Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA
    Mol Vis 12:606-15. 2006
    ..The purpose of this study was to develop and test a relatively simple alternative to custom synthesized nanoparticles for use in multiple biological systems, with special focus on the eye...
  48. ncbi Modeling a whole organ using proteomics: the avian bursa of Fabricius
    Fiona M McCarthy
    Department of Basic Science, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762 6100, USA
    Proteomics 6:2759-71. 2006
    ..Finally, we have developed a novel method for facilitating structural annotation, "expressed peptide sequence tags" (ePSTs) and demonstrate its utility by identifying 521 potential novel proteins from the chicken "unassigned chromosome"...
  49. ncbi [Protective effect of ischemic postconditioning on ischemic reperfusion injury of rat liver graft]
    Nan Wang
    Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, Xi an 710038, China
    Zhonghua Wai Ke Za Zhi 43:1533-6. 2005
    ..To observe the protective effect of ischemic postconditioning on ischemic reperfusion injury of rat liver graft and to investigate the possible mechanism...
  50. ncbi Inhibition of PC-PLC blocked the survival of mouse neural cells by up-regulating the expression of integrin beta4 and Rb
    Xin Lv
    Institute of Developmental Biology, School of Life Science, Shandong University, Jinan, China
    Dev Neurosci 28:499-504. 2006
    ..The changes of intracellular ROS level induced by D609 indicated that a modest level of ROS might be indispensable to neural cell survival...
  51. ncbi Disruption of MHC class II-restricted antigen presentation by vaccinia virus
    Ping Li
    Department of Microbiology and Immunology, Center for Immunobiology, and Walther Oncology Center, Indiana University School of Medicine, and Walther Cancer Institute, Indianapolis, IN 46202, USA
    J Immunol 175:6481-8. 2005
    ..Together, these observations suggest that disruption of MHC class II-mediated Ag presentation may be one of multiple strategies VV has evolved to escape host immune surveillance...
  52. ncbi L55P transthyretin accelerates subunit exchange and leads to rapid formation of hybrid tetramers
    Catherine A Keetch
    Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom
    J Biol Chem 280:41667-74. 2005
    ..This implies interactions between the two proteins that accelerate the formation of hybrid tetramers, a result with important implications for transthyretin amyloidosis...
  53. ncbi [A new target of cancer therapy: advances in the study of histone deacetylase]
    Ai Lin Liu
    Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
    Yao Xue Xue Bao 40:585-90. 2005
  54. pmc Matrix metalloproteinase gelatinase B (MMP-9) is associated with leaking glaucoma filtering blebs
    Shravan K Chintala
    Eye Research Institute, Oakland University, 40g Dodge Hall, Rochester, MI, USA
    Exp Eye Res 81:429-36. 2005
    ..MMP-g may be involved in the mechanism of formation of bleb leaks. Precise description of the cascade of events leading to bleb leakage may allow the design of therapeutic interventions to prevent, stabilize or reverse bleb leakage...
  55. ncbi Cell cycle kinetic dysregulation in HIV-infected normal lymphocytes
    David M Asmuth
    Department of Internal Medicine, University of California Davis, Sacramento, California
    Cytometry A 66:41-51. 2005
    ....
  56. ncbi Estimating cell death in G(2)M using bivariate BrdUrd/DNA flow cytometry
    R Allen White
    Department of Biostatistics and Applied Mathematics, University of Texas MD Anderson Cancer Center, Houston, Texas 77030 4009, USA
    Cytometry A 66:32-40. 2005
    ..However, these methods of analysis do not consider the possibility of cell death. This report demonstrates that estimating cell death in G(2)/M is possible...
  57. ncbi Changes in plasma membrane protein structure after photodynamic action in freshly isolated rat pancreatic acini. An FTIR study
    Nan Wang
    Institute of Cell Biology, Beijing Normal University, Beijing 100875, China
    J Photochem Photobiol B 71:27-34. 2003
    ..Taken together the fact that under the present experimental conditions SALPC mainly localized at the plasma membrane, it is concluded that SALPC photodynamic action directly modulates plasma membrane protein structure...
  58. doi Proteome profile of human breast cancer tissue generated by LC-ESI-MS/MS combined with sequential protein precipitation and solubilization
    Yan Gong
    PolyomX Program, Cross Cancer Institute, Alberta Cancer Board, and Department of Chemistry, Oncology, and Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada
    J Proteome Res 7:3583-90. 2008
    ..95% by using the target-decoy sequence search strategy. On the basis of gene ontology categorization, the identified proteins represented a wide variety of biological functions, cellular processes, and cellular locations...
  59. ncbi Scanning cytometry with a LEAP: laser-enabled analysis and processing of live cells in situ
    Peter Szaniszlo
    Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, USA
    Cytometry A 69:641-51. 2006
    ..A new technology, called LEAP (laser-enabled analysis and processing), offers a unique combination of capabilities in cell purification and selective macromolecule delivery (optoinjection)...
  60. ncbi Suppressing phosphatidylcholine-specific phospholipase C and elevating ROS level, NADPH oxidase activity and Rb level induced neuronal differentiation in mesenchymal stem cells
    Nan Wang
    Institute of Developmental Biology, School of Life Science, Shandong University, Jinan, China
    J Cell Biochem 100:1548-57. 2007
    ..Furthermore, the level of Rb protein was evidently elevated. Our data suggested that PC-PLC mediated neuronal differentiation of rat MSCs by elevating NADPH oxidase activity, ROS level, and up-regulating the expression of Rb protein...
  61. ncbi Constitutive signalling pathway activity in trabecular meshwork cells from glaucomatous eyes
    Xun Zhang
    Evelyn F and William L McKnight Vision Research Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA
    Exp Eye Res 82:968-73. 2006
    ..These results provide new information about the behaviour of glaucomatous TM cells, which may be important for understanding the pathophysiology of high-tension glaucoma...
  62. ncbi Proteome profile of cytosolic component of zebrafish liver generated by LC-ESI MS/MS combined with trypsin digestion and microwave-assisted acid hydrolysis
    Nan Wang
    Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
    J Proteome Res 6:263-72. 2007
    ..Identified proteins--which included several groups of established protein biomarkers--were functionally classified. We discuss the functions and implications of these biomarkers within the context of zebrafish toxicology...
  63. ncbi Improving penetration in tumors with nanoassemblies of phospholipids and doxorubicin
    Ning Tang
    Protein and Peptide Pharmaceutical Laboratory, National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    J Natl Cancer Inst 99:1004-15. 2007
    ..We hypothesized that 10- to 20-nm nanoassemblies of phospholipids containing doxorubicin would improve the drug's penetration, accumulation, and antitumor activity...
  64. ncbi Safrole oxide induces neuronal apoptosis through inhibition of integrin beta4/SOD activity and elevation of ROS/NADPH oxidase activity
    Le Su
    Institute of Developmental Biology, School of Life Science, Shandong University, Jinan, China
    Life Sci 80:999-1006. 2007
    ..Our study thus demonstrates that safrole oxide induces neuronal apoptosis through integrin beta4, ROS, NADPH, and MnSOD...
  65. pmc AgBase: a unified resource for functional analysis in agriculture
    Fiona M McCarthy
    Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, PO Box 6100, Mississippi, MS 39762, USA
    Nucleic Acids Res 35:D599-603. 2007
    ..We encourage and publicly acknowledge GO annotations from researchers and provide an online mechanism for agricultural researchers to submit requests for GO annotations...
  66. pmc Severe acute respiratory syndrome coronavirus infection of mice transgenic for the human Angiotensin-converting enzyme 2 virus receptor
    Chien Te K Tseng
    Department of Microbiology and Immunology, University of Texas Medical Branch, 301 University Boulevard, G 150 Keiller Building, Galveston, TX 77555 0609, USA
    J Virol 81:1162-73. 2007
    ....
  67. ncbi Gene cloning and characterization of a novel alpha-amylase from alkaliphilic Alkalimonas amylolytica
    Nan Wang
    Institute of Microbiology, Chinese Academy of Sciences, Beijing, PR China
    Biotechnol J 1:1258-65. 2006
    ..Taken together, AmyA from A. amylolytica has potential to be used in paper, textile, detergent and other industries where starch needs to be degraded in an alkaline environment...
  68. ncbi Spectroscopic studies on the interaction of azelnidipine with bovine serum albumin
    Nan Wang
    School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, PR China
    Int J Pharm 351:55-60. 2008
    ..The binding of azelnidipine to BSA leads to changes in the conformation of BSA according to synchronous fluorescence spectra and CD data. The presence of metal ion decreases the binding constant of azelnidipine-BSA complex...
  69. pmc ProtQuant: a tool for the label-free quantification of MudPIT proteomics data
    Susan M Bridges
    Department of Computer Science and Engineering, Mississippi State University, Starkville, MS 39762, USA
    BMC Bioinformatics 8:S24. 2007
    ..We describe ProtQuant, a Java-based tool for label-free protein quantification that uses the previously published SigmaXCorr method for quantification and includes an improved method for handling missing data...
  70. ncbi Cloning and characterization of squalene synthase (SQS) gene from Ganoderma lucidum
    Ming Wen Zhao
    College of Life Sciences, Nanjing Agricultural University, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing 210095, Jiangsu, PR China
    J Microbiol Biotechnol 17:1106-12. 2007
    ..Functional complementation of Gl-SQS in a SQS-deficient strain of Saccharomyces cerevisiae confirmed that the cloned cDNA encoded a squalene synthase...
  71. pmc Gene Ontology annotation quality analysis in model eukaryotes
    Teresia J Buza
    Department of Basic Sciences, Mississippi State University, Mississippi 39762, USA
    Nucleic Acids Res 36:e12. 2008
    ..The GAQ score also allows researchers to quantitatively assess the functional data available for their experimental systems (arrays or databases)...
  72. ncbi The failure of torcetrapib: was it the molecule or the mechanism?
    Alan R Tall
    Arterioscler Thromb Vasc Biol 27:257-60. 2007