J F Hunt

Summary

Affiliation: Columbia University
Country: USA

Publications

  1. pmc Structure and dynamics of the P7 protein from the bacteriophage phi 12
    Ertan Eryilmaz
    Department of Chemistry, The City College of New York, 160 Convent Avenue, New York, NY 10031, USA
    J Mol Biol 382:402-22. 2008
  2. ncbi request reprint Ping-pong cross-validation in real space: a method for increasing the phasing power of a partial model without risk of model bias
    John F Hunt
    Howard Hughes Medical Institute and Department of Biochemistry, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9050, USA
    Acta Crystallogr D Biol Crystallogr 59:214-24. 2003
  3. ncbi request reprint Spontaneous, pH-dependent membrane insertion of a transbilayer alpha-helix
    J F Hunt
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511, USA
    Biochemistry 36:15177-92. 1997
  4. ncbi request reprint Nucleotide control of interdomain interactions in the conformational reaction cycle of SecA
    John F Hunt
    Department of Biological Sciences, 702A Fairchild Center, MC2434, Columbia University, New York, NY 10027, USA
    Science 297:2018-26. 2002
  5. ncbi request reprint Crystal structures of the MJ1267 ATP binding cassette reveal an induced-fit effect at the ATPase active site of an ABC transporter
    N Karpowich
    Department of Biological Sciences, 702A Fairchild Center, MC2434, Columbia University, New York, NY 10027, USA
    Structure 9:571-86. 2001
  6. ncbi request reprint Protein solubility and folding monitored in vivo by structural complementation of a genetic marker protein
    W C Wigley
    Department of Physiology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas TX 75235, USA
    Nat Biotechnol 19:131-6. 2001
  7. ncbi request reprint The crystal structure of the MJ0796 ATP-binding cassette. Implications for the structural consequences of ATP hydrolysis in the active site of an ABC transporter
    Y R Yuan
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    J Biol Chem 276:32313-21. 2001
  8. ncbi request reprint Structural adaptations in the specialized bacteriophage T4 co-chaperonin Gp31 expand the size of the Anfinsen cage
    J F Hunt
    Howard Hughes Medical Institute and Department of Biochemistry, The University of Texas Southwestern Medical Center, Dallas 75235 9050, USA
    Cell 90:361-71. 1997
  9. ncbi request reprint Glycophorin A dimerization is driven by specific interactions between transmembrane alpha-helices
    M A Lemmon
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511
    J Biol Chem 267:7683-9. 1992
  10. ncbi request reprint Cell biology: shape-shifting protein channel
    Jordi Benach
    Nature 427:24-6. 2004

Collaborators

Detail Information

Publications37

  1. pmc Structure and dynamics of the P7 protein from the bacteriophage phi 12
    Ertan Eryilmaz
    Department of Chemistry, The City College of New York, 160 Convent Avenue, New York, NY 10031, USA
    J Mol Biol 382:402-22. 2008
    ..Given the requirement of P7 in maintaining genome packaging efficiency and transcriptional fidelity, our data suggest a central biological role for P7-RNA interactions...
  2. ncbi request reprint Ping-pong cross-validation in real space: a method for increasing the phasing power of a partial model without risk of model bias
    John F Hunt
    Howard Hughes Medical Institute and Department of Biochemistry, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9050, USA
    Acta Crystallogr D Biol Crystallogr 59:214-24. 2003
    ..Real-space cross-validation can be applied to reduce the risk of model bias associated with partial model phasing, streamlining this approach and expanding its range of applicability...
  3. ncbi request reprint Spontaneous, pH-dependent membrane insertion of a transbilayer alpha-helix
    J F Hunt
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511, USA
    Biochemistry 36:15177-92. 1997
    ..With a pKa of 6.0, the peptide inserts into the bilayer as a transbilayer alpha-helix. The insertion reaction proceeds rapidly at room temperature and is fully reversible...
  4. ncbi request reprint Nucleotide control of interdomain interactions in the conformational reaction cycle of SecA
    John F Hunt
    Department of Biological Sciences, 702A Fairchild Center, MC2434, Columbia University, New York, NY 10027, USA
    Science 297:2018-26. 2002
    ....
  5. ncbi request reprint Crystal structures of the MJ1267 ATP binding cassette reveal an induced-fit effect at the ATPase active site of an ABC transporter
    N Karpowich
    Department of Biological Sciences, 702A Fairchild Center, MC2434, Columbia University, New York, NY 10027, USA
    Structure 9:571-86. 2001
    ..Previous structures have defined the fold of the ABC and established the flexibility of its alpha-helical subdomain. But the nature of the mechanical changes that occur at each step of the chemical ATPase cycle have not been defined...
  6. ncbi request reprint Protein solubility and folding monitored in vivo by structural complementation of a genetic marker protein
    W C Wigley
    Department of Physiology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas TX 75235, USA
    Nat Biotechnol 19:131-6. 2001
    ..Thus, structural complementation provides a means of monitoring protein solubility/misfolding in vivo, and should find utility in the screening for compounds that influence the pathological consequences of these processes...
  7. ncbi request reprint The crystal structure of the MJ0796 ATP-binding cassette. Implications for the structural consequences of ATP hydrolysis in the active site of an ABC transporter
    Y R Yuan
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    J Biol Chem 276:32313-21. 2001
    ....
  8. ncbi request reprint Structural adaptations in the specialized bacteriophage T4 co-chaperonin Gp31 expand the size of the Anfinsen cage
    J F Hunt
    Howard Hughes Medical Institute and Department of Biochemistry, The University of Texas Southwestern Medical Center, Dallas 75235 9050, USA
    Cell 90:361-71. 1997
    ....
  9. ncbi request reprint Glycophorin A dimerization is driven by specific interactions between transmembrane alpha-helices
    M A Lemmon
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511
    J Biol Chem 267:7683-9. 1992
    ..This approach allows the interface between interacting helices to be defined...
  10. ncbi request reprint Cell biology: shape-shifting protein channel
    Jordi Benach
    Nature 427:24-6. 2004
  11. ncbi request reprint Nucleotide exchange from the high-affinity ATP-binding site in SecA is the rate-limiting step in the ATPase cycle of the soluble enzyme and occurs through a specialized conformational state
    John J Fak
    Department of Biological Sciences, 702A Fairchild Center, MC2434, Columbia University, New York, New York 10027, USA
    Biochemistry 43:7307-27. 2004
    ....
  12. ncbi request reprint Dynamics of ATP-binding cassette contribute to allosteric control, nucleotide binding and energy transduction in ABC transporters
    Chunyu Wang
    Department of Biochemistry and Molecular Biophysics, Columbia University, 630 W 168th St, New York, NY 10032, USA
    J Mol Biol 342:525-37. 2004
    ..The restriction of correlated protein motions is likely a central mechanism for allosteric communications. Comparison between dynamics data from NMR and X-ray crystallography reveals their overall consistency and complementarity...
  13. ncbi request reprint The SufE sulfur-acceptor protein contains a conserved core structure that mediates interdomain interactions in a variety of redox protein complexes
    Sharon Goldsmith-Fischman
    Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    J Mol Biol 344:549-65. 2004
    ..Therefore, the core fold of SufE/IscU has been adapted to mediate interdomain interactions in diverse redox protein systems in the course of evolution...
  14. ncbi request reprint Impact of the deltaF508 mutation in first nucleotide-binding domain of human cystic fibrosis transmembrane conductance regulator on domain folding and structure
    Hal A Lewis
    Structural GenomiX, Inc, San Diego, California 92121, USA
    J Biol Chem 280:1346-53. 2005
    ....
  15. ncbi request reprint Role of a conserved glutamate residue in the Escherichia coli SecA ATPase mechanism
    Christopher R Zito
    Molecular Biology and Biochemistry Department, Wesleyan University, Middletown, Connecticut 06459, USA
    J Biol Chem 280:14611-9. 2005
    ....
  16. ncbi request reprint Crystal structures of two bacterial 3-hydroxy-3-methylglutaryl-CoA lyases suggest a common catalytic mechanism among a family of TIM barrel metalloenzymes cleaving carbon-carbon bonds
    Farhad Forouhar
    Department of Biological Sciences and Northeast Structural Genomics Consortium, Columbia University, New York, NY 10027, USA
    J Biol Chem 281:7533-45. 2006
    ..2006) J. Biol. Chem. 281, 7526-7532). Our model is consistent with extensive mutagenesis results and can guide subsequent studies directed at definitive experimental elucidation of this enzyme's reaction mechanism...
  17. ncbi request reprint Crystal structure of human 3-hydroxy-3-methylglutaryl-CoA Lyase: insights into catalysis and the molecular basis for hydroxymethylglutaric aciduria
    Zhuji Fu
    Department of Biochemistry, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    J Biol Chem 281:7526-32. 2006
    ..The structure provides a rationale for the decrease in enzyme activity due to clinical mutations, including H233R, R41Q, D42H, and D204N, that compromise active site function or enzyme stability...
  18. ncbi request reprint Crystal structures of catalytic complexes of the oxidative DNA/RNA repair enzyme AlkB
    Bomina Yu
    Department of Biological Sciences and Northeast Structural Genomics Consortium, 702A Fairchild Center, MC2434, Columbia University, New York, New York 10027, USA
    Nature 439:879-84. 2006
    ..These observations suggest that protein dynamics modulate redox chemistry and that a hypothesized migration of the reactive oxy-ferryl ligand on the catalytic Fe ion may be impeded when the protein is constrained in the crystal lattice...
  19. ncbi request reprint Disorder breathes life into a DEAD motor
    Lorraine F Cavanaugh
    Nat Struct Mol Biol 13:566-9. 2006
  20. pmc The structural basis of cyclic diguanylate signal transduction by PilZ domains
    Jordi Benach
    Department of Biological Sciences, Northeast Structural Genomics Consortium, Columbia University, New York, NY 10027, USA
    EMBO J 26:5153-66. 2007
    ..The very small size of the N-terminal conformational switch is likely to explain the facile evolutionary diversification of the PilZ domain...
  21. pmc Structure of nucleotide-binding domain 1 of the cystic fibrosis transmembrane conductance regulator
    Hal A Lewis
    Structural GenomiX Inc, San Diego, CA, USA
    EMBO J 23:282-93. 2004
    ..Our results are consistent with a CFTR mechanism, whereby channel gating occurs through ATP binding in an NBD1-NBD2 nucleotide sandwich that forms upon displacement of NBD1 regulatory segments...
  22. ncbi request reprint Bacillus subtilis SecA ATPase exists as an antiparallel dimer in solution
    Haiyuan Ding
    Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, Connecticut 06459, USA
    Biochemistry 42:8729-38. 2003
    ....
  23. ncbi request reprint The 2.3-A crystal structure of the shikimate 5-dehydrogenase orthologue YdiB from Escherichia coli suggests a novel catalytic environment for an NAD-dependent dehydrogenase
    Jordi Benach
    Department of Biological Sciences and Northeast Structural Genomics Consortium, Columbia University, New York, New York 10027, USA
    J Biol Chem 278:19176-82. 2003
    ..The nature and disposition of the active site residues suggest a novel reaction mechanism in which an aspartate acts as the general acid/base catalyst during the hydride transfer reaction...
  24. pmc Cooperative, ATP-dependent association of the nucleotide binding cassettes during the catalytic cycle of ATP-binding cassette transporters
    Jonathan E Moody
    Department of Physiology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390 9040, USA
    J Biol Chem 277:21111-4. 2002
    ..Thus, upon hydrolysis of bound ATP and the release of product, both electrostatic and conformational changes drive the cassettes apart, providing a second opportunity to couple free energy changes to the transport reaction...
  25. ncbi request reprint The crystal structure of the olfactory marker protein at 2.3 A resolution
    Paul C Smith
    Department of Biological Sciences, 702A Fairchild Center, MC 2434, Columbia University, New York, NY 10027, USA
    J Mol Biol 319:807-21. 2002
    ....
  26. pmc ATP binding to the motor domain from an ABC transporter drives formation of a nucleotide sandwich dimer
    Paul C Smith
    Department of Biological Sciences, 702A Fairchild Center, MC2434, Columbia University, New York, New York 10027, USA
    Mol Cell 10:139-49. 2002
    ..These results establish the stereochemical basis of the power stroke of ABC transporter pumps...
  27. ncbi request reprint Phospholipid-induced monomerization and signal-peptide-induced oligomerization of SecA
    Jordi Benach
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    J Biol Chem 278:3628-38. 2003
    ..Cycles of SecA subunit recruitment and dissociation could potentially be employed to achieve processivity in polypeptide transport...
  28. ncbi request reprint The crystal structure of MT0146/CbiT suggests that the putative precorrin-8w decarboxylase is a methyltransferase
    Jacob P Keller
    Department of Biological Sciences, 702A Fairchild Center, MC2434, Columbia University, New York, NY 10027, USA
    Structure 10:1475-87. 2002
    ..Therefore, MT0146/CbiT probably functions as a precorrin methyltransferase and represents the first enzyme identified with this activity that does not have the canonical precorrin methyltransferase fold...
  29. ncbi request reprint Crystal structures of the BtuF periplasmic-binding protein for vitamin B12 suggest a functionally important reduction in protein mobility upon ligand binding
    Nathan K Karpowich
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    J Biol Chem 278:8429-34. 2003
    ..Furthermore, the high mobility of this domain in free BtuF could provide an entropic driving force for the subsequent release of BtuF required to complete the transport cycle...
  30. ncbi request reprint Structural characterization of an MJ1267 ATP-binding cassette crystal with a complex pattern of twinning caused by promiscuous fiber packing
    Yu Ren Yuan
    Department of Biological Sciences, 702A Fairchild Center, MC2434, Columbia University, New York, NY 10027, USA
    Acta Crystallogr D Biol Crystallogr 59:225-38. 2003
    ....
  31. ncbi request reprint Assessment of protein folding/solubility in live cells
    Rhesa D Stidham
    Department of Physiology, Graduate Program in Molecular Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, USA
    Methods Mol Biol 205:155-69. 2003
  32. ncbi request reprint The 2.35 A structure of the TenA homolog from Pyrococcus furiosus supports an enzymatic function in thiamine metabolism
    Jordi Benach
    Department of Biological Sciences and Northeast Structural Genomics Consortium, 702A Fairchild Center, MC2434, Columbia University, New York, NY 10027, USA
    Acta Crystallogr D Biol Crystallogr 61:589-98. 2005
    ..Therefore, the crystal structure of PF1337 supports the hypothesis that TenA homologs have an indirect effect in altering gene-expression patterns and function instead as enzymes involved in thiamine metabolism...
  33. ncbi request reprint 1H, 15N and 13C backbone assignment of MJ1267, an ATP-binding cassette
    Chunyu Wang
    J Biomol NMR 24:167-8. 2002
  34. pmc The perplexing challenges of a pump turned channel
    John F Hunt
    Department of Biological Sciences, 702A Fairchild Center, MC2434, Columbia University, New York, NY 10027, USA
    J Physiol 539:331. 2002
  35. ncbi request reprint Mutations that change the position of the putative gamma-phosphate linker in the nucleotide binding domains of CFTR alter channel gating
    Allan L Berger
    Howard Hughes Medical Institute, Departments of Internal Medicine and Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
    J Biol Chem 277:2125-31. 2002
    ..Our results extend models of nucleotide-induced structural changes from bacterial NBDs to a functional mammalian ATP-binding cassette transporter...
  36. pmc Concentration-dependent effects of endogenous S-nitrosoglutathione on gene regulation by specificity proteins Sp3 and Sp1
    Khalequz Zaman
    Division of Pulmonary Medicine, Department of Pediatrics, University of Virginia School of Medicine, P O Box 800356, Charlottesville, VA 22908, USA
    Biochem J 380:67-74. 2004
    ....
  37. ncbi request reprint A protein sequence that can encode native structure by disfavoring alternate conformations
    W Christian Wigley
    Department of Physiology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390 9040, USA
    Nat Struct Biol 9:381-8. 2002
    ....