WAYNE HENDRICKSON

Summary

Affiliation: Columbia University
Country: USA

Publications

  1. ncbi Synchrotron crystallography
    W A Hendrickson
    Dept of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University, New York, NY 10032, USA
    Trends Biochem Sci 25:637-43. 2000
  2. ncbi Impact of structures from the protein structure initiative
    Wayne A Hendrickson
    Howard Hughes Medical Institute, Columbia University, New York, NY 10032, USA
    Structure 15:1528-9. 2007
  3. ncbi Transduction of biochemical signals across cell membranes
    Wayne A Hendrickson
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Q Rev Biophys 38:321-30. 2005
  4. ncbi Large-scale production of a disulfide-stabilized constitutively active mutant opsin
    K Christopher Min
    Department of Neurology, Columbia University, New York, NY 10032, USA
    Protein Expr Purif 75:236-41. 2011
  5. ncbi A force field for virtual atom molecular mechanics of proteins
    Anil Korkut
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 106:15667-72. 2009
  6. ncbi Structure of the entire cytoplasmic portion of a sensor histidine-kinase protein
    Alberto Marina
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    EMBO J 24:4247-59. 2005
  7. ncbi Structural characterization of the predominant family of histidine kinase sensor domains
    Zhen Zhang
    Howard Hughes Medical Institute, Columbia University, New York, NY 10032, USA
    J Mol Biol 400:335-53. 2010
  8. ncbi Production and characterization of monoclonal antibodies sensitive to conformation in the 5HT2c serotonin receptor
    Filippo Mancia
    Department of Biochemistry and Molecular Biophysics and the Howard Hughes Medical Institute, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 104:4303-8. 2007
  9. ncbi Computation of conformational transitions in proteins by virtual atom molecular mechanics as validated in application to adenylate kinase
    Anil Korkut
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 106:15673-8. 2009
  10. ncbi Crystal structure of a functional dimer of the PhoQ sensor domain
    Jonah Cheung
    Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University, New York, New York 10032, USA
    J Biol Chem 283:13762-70. 2008

Research Grants

  1. Structural Biology of G-Protein Coupled Receptors
    WAYNE HENDRICKSON; Fiscal Year: 2009
  2. Structural Biology of G-Protein Coupled Receptors
    WAYNE HENDRICKSON; Fiscal Year: 2007
  3. ANOMALOUS-SCATTERING STUDIES OF MACROMOLECULAR STRUCTURE
    WAYNE HENDRICKSON; Fiscal Year: 2007
  4. Structural Biology of G-Protein Coupled Receptors
    WAYNE HENDRICKSON; Fiscal Year: 2006
  5. Structural Biology of G-Protein Coupled Receptors
    Wayne A Hendrickson; Fiscal Year: 2010

Collaborators

  • ERIC SCHON
  • ERNESTO I FREIRE
  • RICHARD BERT VALLEE
  • Jonah Cheung
  • Qing R Fan
  • John C Williams
  • Filippo Mancia
  • Anil Korkut
  • K Christopher Min
  • Peter D Kwong
  • Zahra Assur
  • Zhen Zhang
  • Joseph Sodroski
  • Richard Wyatt
  • Jason O Moore
  • Ira Schieren
  • Carey D Waldburger
  • Erik Martinez-Hackert
  • Chih Chin Huang
  • Tongqing Zhou
  • Alberto Marina
  • Shahzad Majeed
  • James Robinson
  • Michael L Doyle
  • Shi Hua Xiang
  • David J Casper
  • Liping Wang
  • Yan Jin
  • Craig A Bingman
  • Marsha Reyngold
  • Risa Siegel
  • Yonghua Sun
  • Michael C Fitzgerald
  • Petra L Roulhac
  • Anindya G Roy
  • Monica Mendelsohn
  • Richard Axel
  • Susan Brenner-Morton
  • Yuri Sykulev
  • Bruce D Walker
  • Nadia Anikeeva
  • Spyros A Kalams
  • Christian Roumestand
  • Julie M Decker
  • Chih-chin Huang
  • Carolyn Sue
  • Philippe Barthe
  • George M Shaw
  • Graham S Banting
  • D Moira Glerum
  • Dean H Hamer
  • Claudio Vita
  • Quentin J Sattentau
  • Hua Yang
  • Loic Martin
  • Hui Xie
  • Francois Stricher
  • Rhett A Kovall
  • James Arthos
  • Michael Fung
  • Donald Van Ryk
  • Rishi Gupta
  • Dennis R Burton
  • Shi-Hua Xiang
  • Paul W I H Parren
  • Irwin Chaiken
  • Claudia Cicala
  • Miro Venturi
  • Hermann Katinger
  • Stephanie A Leavitt
  • Tavis D Steenbeke
  • Carlo D Rizzuto

Detail Information

Publications29

  1. ncbi Synchrotron crystallography
    W A Hendrickson
    Dept of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University, New York, NY 10032, USA
    Trends Biochem Sci 25:637-43. 2000
    ..Both the high-throughput applications of structural genomics and also the challenging studies of macromolecular machinery are expected to flourish...
  2. ncbi Impact of structures from the protein structure initiative
    Wayne A Hendrickson
    Howard Hughes Medical Institute, Columbia University, New York, NY 10032, USA
    Structure 15:1528-9. 2007
  3. ncbi Transduction of biochemical signals across cell membranes
    Wayne A Hendrickson
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Q Rev Biophys 38:321-30. 2005
    ..Biophysical principles that govern the processes of signal transduction across cell membranes are also discussed...
  4. ncbi Large-scale production of a disulfide-stabilized constitutively active mutant opsin
    K Christopher Min
    Department of Neurology, Columbia University, New York, NY 10032, USA
    Protein Expr Purif 75:236-41. 2011
    ..The purified mutant opsin is able to activate transducin to a higher degree than opsin and may prove useful for future structural studies of the active state of GPCRs...
  5. ncbi A force field for virtual atom molecular mechanics of proteins
    Anil Korkut
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 106:15667-72. 2009
    ..These findings suggest that the virtual atom force field is a suitable tool for various molecular mechanics applications on large macromolecular systems undergoing large conformational changes...
  6. ncbi Structure of the entire cytoplasmic portion of a sensor histidine-kinase protein
    Alberto Marina
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    EMBO J 24:4247-59. 2005
    ..Mutational tests support the functional relevance of interdomain contacts...
  7. ncbi Structural characterization of the predominant family of histidine kinase sensor domains
    Zhen Zhang
    Howard Hughes Medical Institute, Columbia University, New York, NY 10032, USA
    J Mol Biol 400:335-53. 2010
    ..Some HK1 family proteins prove to be sensors for chemotaxis proteins or diguanylate cyclase receptors, implying a combinatorial molecular evolution...
  8. ncbi Production and characterization of monoclonal antibodies sensitive to conformation in the 5HT2c serotonin receptor
    Filippo Mancia
    Department of Biochemistry and Molecular Biophysics and the Howard Hughes Medical Institute, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 104:4303-8. 2007
    ..Five of the nine mAbs have cytoplasmic epitopes, and two of these are sensitive to the ligand state of the receptor. These properties should be useful both for structural analysis and in probes of function...
  9. ncbi Computation of conformational transitions in proteins by virtual atom molecular mechanics as validated in application to adenylate kinase
    Anil Korkut
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 106:15673-8. 2009
    ..We expect that the VAMM algorithm can address many biological systems...
  10. ncbi Crystal structure of a functional dimer of the PhoQ sensor domain
    Jonah Cheung
    Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University, New York, New York 10032, USA
    J Biol Chem 283:13762-70. 2008
    ..The physiological importance of the salt bridge to in vivo PhoQ function has been confirmed by mutagenesis. The mutant structure has an alternative, non-physiological dimeric association...
  11. ncbi Crystal structures of C4-dicarboxylate ligand complexes with sensor domains of histidine kinases DcuS and DctB
    Jonah Cheung
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA
    J Biol Chem 283:30256-65. 2008
    ..Our work reveals that despite disparate sizes, both structures contain a similar characteristic alpha/beta PDC (PhoQ-DcuS-CitA) sensor-domain fold and display similar modes of ligand binding, suggesting similar mechanisms of function...
  12. ncbi Structural analysis of ligand stimulation of the histidine kinase NarX
    Jonah Cheung
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Structure 17:190-201. 2009
    ..Such conformational changes might represent a conserved mechanism of signaling in histidine kinases by which ligand binding is communicated across the lipid bilayer...
  13. ncbi Crystal structure of human SCO1: implications for redox signaling by a mitochondrial cytochrome c oxidase "assembly" protein
    John C Williams
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA
    J Biol Chem 280:15202-11. 2005
    ..These data suggests that SCO functions not as a COX copper chaperone, but rather as a mitochondrial redox signaling molecule...
  14. ncbi Structural analysis of sensor domains from the TMAO-responsive histidine kinase receptor TorS
    Jason O Moore
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Structure 17:1195-204. 2009
    ..Isolated TorS sensor domains dimerize in solution; and from comparisons with dimeric NarX and Tar sensors, we postulate that signaling through TorS dimers involves a piston-type displacement between helices...
  15. ncbi Sensor domains of two-component regulatory systems
    Jonah Cheung
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Curr Opin Microbiol 13:116-23. 2010
    ..Subtle rearrangements along a structurally labile dimer interface, in the form of possible sliding or rotational motions, are propagated from the sensor domain to the transmitter domain to modulate activity of the receptor...
  16. ncbi Comparative structural analysis of the binding domain of follicle stimulating hormone receptor
    Qing R Fan
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Proteins 72:393-401. 2008
    ..Analysis of the ligand-binding site of LRR-containing proteins reveals that they generally utilize extensive interface area and a large number of charged residues to facilitate high-affinity protein-protein interactions...
  17. ncbi Expression of recombinant G-protein coupled receptors for structural biology
    Filippo Mancia
    Howard Hughes Medical Institute and Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Mol Biosyst 3:723-34. 2007
  18. ncbi Two-color selection for amplified co-production of proteins in mammalian cells
    Zahra Assur
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Protein Expr Purif 55:319-24. 2007
    ..This manner of efficient selection may readily be adapted for the co-production of other complexes of two or more proteins...
  19. ncbi Structure of human follicle-stimulating hormone in complex with its receptor
    Qing R Fan
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA
    Nature 433:269-77. 2005
    ..The FSH-FSHR(HB) complexes form dimers in the crystal and at high concentrations in solution. Such dimers may participate in transmembrane signal transduction...
  20. ncbi Crystal structure of dynein light chain TcTex-1
    John C Williams
    Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University, New York, NY 10032, USA
    J Biol Chem 280:21981-6. 2005
    ....
  21. ncbi Structural basis for degenerate recognition of natural HIV peptide variants by cytotoxic lymphocytes
    Erik Martinez-Hackert
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    J Biol Chem 281:20205-12. 2006
    ....
  22. ncbi Crystal structure of human alpha-tocopherol transfer protein bound to its ligand: implications for ataxia with vitamin E deficiency
    K Christopher Min
    Howard Hughes Medical Institute and Department of Neurology, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 100:14713-8. 2003
    ..We propose that this positively charged surface may serve to orient an interacting protein, which might function to regulate the release of alpha-T through an induced change in conformation of ATTP...
  23. ncbi Structural biology of glycoprotein hormones and their receptors
    Qing R Fan
    Department of Biochemistry and Molecular Biophysics, Columbia University Medical Center, New York, NY 10032, USA
    Endocrine 26:179-88. 2005
    ....
  24. ncbi Mutagenic stabilization and/or disruption of a CD4-bound state reveals distinct conformations of the human immunodeficiency virus type 1 gp120 envelope glycoprotein
    Shi Hua Xiang
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute and Harvard Medical School Boston, Massachusetts 02115, USA
    J Virol 76:9888-99. 2002
    ..These results indicate that CD4BS antibodies recognize conformations of gp120 different from that recognized by CD4 and CD4i antibodies...
  25. ncbi Scorpion-toxin mimics of CD4 in complex with human immunodeficiency virus gp120 crystal structures, molecular mimicry, and neutralization breadth
    Chih Chin Huang
    Vaccine Research Center, National Institutes of Health, Bethesda, Maryland 20892, USA
    Structure 13:755-68. 2005
    ....
  26. ncbi Interfacial metal and antibody recognition
    Tongqing Zhou
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:14575-80. 2005
    ..5 kcal/mol of binding energy. This energetic contribution, which is greater than that from a typical protein atom, demonstrates how interfacial metal ligation can play a unique role in antigen recognition...
  27. ncbi Exchange we can believe in
    Wayne A Hendrickson
    Structure 16:1153-5. 2008
  28. ncbi HIV-1 evades antibody-mediated neutralization through conformational masking of receptor-binding sites
    Peter D Kwong
    Vaccine Research Center, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 420:678-82. 2002
    ..Because this solution is available for cell-surface receptors but not for most antibodies, conformational masking enables HIV-1 to maintain receptor binding and simultaneously to resist neutralization...
  29. ncbi Structural and thermodynamic characterization of a cytoplasmic dynein light chain-intermediate chain complex
    John C Williams
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 104:10028-33. 2007
    ..These data suggest that, in the dynein complex, the LCs do not bind cargo, in apparent disagreement with a role for LCs in dynein cargo binding interactions...

Research Grants12

  1. Structural Biology of G-Protein Coupled Receptors
    WAYNE HENDRICKSON; Fiscal Year: 2009
    ..GPCRs are actual and prospective pharmaceutical targets. ..
  2. Structural Biology of G-Protein Coupled Receptors
    WAYNE HENDRICKSON; Fiscal Year: 2007
    ..g. serotonin receptor involvement in disorders of mood such as depression, and GPCRs provide the sites of action for a number of pharmaceutical reagents. ..
  3. ANOMALOUS-SCATTERING STUDIES OF MACROMOLECULAR STRUCTURE
    WAYNE HENDRICKSON; Fiscal Year: 2007
    ..Biologically exciting problems motivate the development of appropriate tools, and forefront methods to accelerate the solution of interesting structures. ..
  4. Structural Biology of G-Protein Coupled Receptors
    WAYNE HENDRICKSON; Fiscal Year: 2006
    ..g. serotonin receptor involvement in disorders of mood such as depression, and GPCRs provide the sites of action for a number of pharmaceutical reagents. ..
  5. Structural Biology of G-Protein Coupled Receptors
    Wayne A Hendrickson; Fiscal Year: 2010
    ..GPCRs are actual and prospective pharmaceutical targets. ..