Joachim Frank

Summary

Affiliation: Columbia University
Country: USA

Publications

  1. pmc Intermediate states during mRNA-tRNA translocation
    Joachim Frank
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, 650 W 168th Street, New York, NY 10032, United States
    Curr Opin Struct Biol 22:778-85. 2012
  2. pmc Single-particle reconstruction of biological macromolecules in electron microscopy--30 years
    Joachim Frank
    The Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA
    Q Rev Biophys 42:139-58. 2009
  3. pmc Structure and dynamics of a processive Brownian motor: the translating ribosome
    Joachim Frank
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York City, New York 10032, USA
    Annu Rev Biochem 79:381-412. 2010
  4. pmc Cryoelectron microscopy structures of the ribosome complex in intermediate states during tRNA translocation
    Jie Fu
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 108:4817-21. 2011
  5. pmc Recognition of aminoacyl-tRNA: a common molecular mechanism revealed by cryo-EM
    Wen Li
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    EMBO J 27:3322-31. 2008
  6. ncbi request reprint Incorporation of aminoacyl-tRNA into the ribosome as seen by cryo-electron microscopy
    Mikel Valle
    Howard Hughes Medical Institute, Health Research, Inc at the Wadsworth Center, Empire State Plaza, Albany, New York 12201 0509, USA
    Nat Struct Biol 10:899-906. 2003
  7. ncbi request reprint RF3 induces ribosomal conformational changes responsible for dissociation of class I release factors
    Haixiao Gao
    Howard Hughes Medical Institute, Health Research, Inc at the Wadsworth Center, Empire State Plaza, Albany, NY 12201 0509, USA
    Cell 129:929-41. 2007
  8. ncbi request reprint Visualizing tmRNA entry into a stalled ribosome
    Mikel Valle
    Howard Hughes Medical Institute, Wadsworth Center, Health Research, Inc, Empire State Plaza, Albany, NY 12201 0509, USA
    Science 300:127-30. 2003
  9. pmc Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation
    Christian M T Spahn
    Wadsworth Center, Health Research Inc, Howard Hughes Medical Institute, Albany, NY 12201 0509, USA
    EMBO J 23:1008-19. 2004
  10. ncbi request reprint Elongation arrest by SecM via a cascade of ribosomal RNA rearrangements
    Kakoli Mitra
    Howard Hughes Medical Institute, Health Research, Inc, at the Wadsworth Center, Empire State Plaza, Albany, New York 12201, USA
    Mol Cell 22:533-43. 2006

Research Grants

  1. STRUCTURAL ANALYSIS OF MACROMOLECULAR ASSEMBLY
    Joachim Frank; Fiscal Year: 2010
  2. STRUCTURAL ANALYSIS OF MACROMOLECULAR ASSEMBLY
    Joachim Frank; Fiscal Year: 2009
  3. EXPERIMENTALLY GUIDED RIBOSOMAL RNA MODELING
    Joachim Frank; Fiscal Year: 2009
  4. EXPERIMENTALLY GUIDED RIBOSOMAL RNA MODELING
    Joachim Frank; Fiscal Year: 2004
  5. EXPERIMENTALLY GUIDED RIBOSOMAL RNA MODELING
    Joachim Frank; Fiscal Year: 2000
  6. EXPERIMENTALLY GUIDED RIBOSOMAL RNA MODELING
    Joachim Frank; Fiscal Year: 2010

Collaborators

Detail Information

Publications52

  1. pmc Intermediate states during mRNA-tRNA translocation
    Joachim Frank
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, 650 W 168th Street, New York, NY 10032, United States
    Curr Opin Struct Biol 22:778-85. 2012
    ....
  2. pmc Single-particle reconstruction of biological macromolecules in electron microscopy--30 years
    Joachim Frank
    The Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA
    Q Rev Biophys 42:139-58. 2009
    ..Increasingly, cryo-EM and single-particle reconstruction, in combination with classification techniques, is revealing dynamic information on functional molecular machines uninhibited by molecular contacts...
  3. pmc Structure and dynamics of a processive Brownian motor: the translating ribosome
    Joachim Frank
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York City, New York 10032, USA
    Annu Rev Biochem 79:381-412. 2010
    ....
  4. pmc Cryoelectron microscopy structures of the ribosome complex in intermediate states during tRNA translocation
    Jie Fu
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 108:4817-21. 2011
    ..Our observations suggest a mechanism by which the rate of translocation can be regulated...
  5. pmc Recognition of aminoacyl-tRNA: a common molecular mechanism revealed by cryo-EM
    Wen Li
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    EMBO J 27:3322-31. 2008
    ....
  6. ncbi request reprint Incorporation of aminoacyl-tRNA into the ribosome as seen by cryo-electron microscopy
    Mikel Valle
    Howard Hughes Medical Institute, Health Research, Inc at the Wadsworth Center, Empire State Plaza, Albany, New York 12201 0509, USA
    Nat Struct Biol 10:899-906. 2003
    ..From these new findings we propose a mechanism that can explain the sequence of events during the decoding of mRNA on the ribosome...
  7. ncbi request reprint RF3 induces ribosomal conformational changes responsible for dissociation of class I release factors
    Haixiao Gao
    Howard Hughes Medical Institute, Health Research, Inc at the Wadsworth Center, Empire State Plaza, Albany, NY 12201 0509, USA
    Cell 129:929-41. 2007
    ....
  8. ncbi request reprint Visualizing tmRNA entry into a stalled ribosome
    Mikel Valle
    Howard Hughes Medical Institute, Wadsworth Center, Health Research, Inc, Empire State Plaza, Albany, NY 12201 0509, USA
    Science 300:127-30. 2003
    ..The structure reveals how tmRNA could move through the ribosome despite its complicated topology and also suggests roles for proteins S1 and SmpB in the function of tmRNA...
  9. pmc Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation
    Christian M T Spahn
    Wadsworth Center, Health Research Inc, Howard Hughes Medical Institute, Albany, NY 12201 0509, USA
    EMBO J 23:1008-19. 2004
    ..sordarin complex that, in contrast to Escherichia coli 70S ribosomes, is also present in vacant 80S ribosomes. A model is suggested, according to which the RSR is part of a mechanism for moving the tRNAs during the translocation reaction...
  10. ncbi request reprint Elongation arrest by SecM via a cascade of ribosomal RNA rearrangements
    Kakoli Mitra
    Howard Hughes Medical Institute, Health Research, Inc, at the Wadsworth Center, Empire State Plaza, Albany, New York 12201, USA
    Mol Cell 22:533-43. 2006
    ..Thus, the ribosome may itself have the ability to regulate its progression through translation by modulating its structure and consequently its receptivity to activation by cofactors...
  11. ncbi request reprint Locking and unlocking of ribosomal motions
    Mikel Valle
    Howard Hughes Medical Institute, and Health Research Incororated at the Wadswoth Center, State University of New York, Albany, 12201, USA
    Cell 114:123-34. 2003
    ..These ribosomal motions can occur only when the P-site tRNA is deacylated. Prior to peptidyl-transfer to the A-site tRNA or peptide removal, the presence of the charged P-site tRNA locks the ribosome and prohibits both of these motions...
  12. pmc Cryo-EM visualization of transfer messenger RNA with two SmpBs in a stalled ribosome
    Sukhjit Kaur
    Howard Hughes Medical Institute, New York State Department of Health, Empire State Plaza, Albany, NY 12201 0509, USA
    Proc Natl Acad Sci U S A 103:16484-9. 2006
    ..The map clarifies that the TLD is located near helix 34 and protein S19 of the 30S subunit, rather than in the A site as tRNA for normal translation, so that the TLD is oriented toward the ORF...
  13. ncbi request reprint Interactions of the release factor RF1 with the ribosome as revealed by cryo-EM
    Urmila Rawat
    Howard Hughes Medical Institute, University of Michigan School of Medicine, 1150 West Medical Ctr Drive, Ann Arbor, MI 48109, USA
    J Mol Biol 357:1144-53. 2006
    ..Very recent data obtained with small-angle X-ray scattering now reveal that the solution structure of RF1 is open, as here seen on the ribosome by cryo-EM, and not closed, as seen in the crystal...
  14. ncbi request reprint The role of tRNA as a molecular spring in decoding, accommodation, and peptidyl transfer
    Joachim Frank
    Howard Hughes Medical Institute, Post Office Box 509, Albany, New York 12201 0509, USA
    FEBS Lett 579:959-62. 2005
    ..Recent findings show that the tRNA structure itself undergoes large conformational changes as well, and that the decoding process must be seen as a complex dynamic interplay between tRNA and the ribosome...
  15. ncbi request reprint Study of the structural dynamics of the E coli 70S ribosome using real-space refinement
    Haixiao Gao
    Howard Hughes Medical Institute, Health Research, Inc, Empire State Plaza, Albany, NY 12201, USA
    Cell 113:789-801. 2003
    ....
  16. pmc tmRNA on its way through the ribosome: two steps of resume, and what next?
    Jie Fu
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA
    RNA Biol 8:586-90. 2011
    ....
  17. ncbi request reprint Dynamics of EF-G interaction with the ribosome explored by classification of a heterogeneous cryo-EM dataset
    Haixiao Gao
    Howard Hughes Medical Institute, Health Research, Inc, Empire State Plaza, Albany, NY 12201 0509, USA
    J Struct Biol 147:283-90. 2004
    ..The possible presence of additional intermediate states is discussed...
  18. pmc Specific interaction between EF-G and RRF and its implication for GTP-dependent ribosome splitting into subunits
    Ning Gao
    Howard Hughes Medical Institute, Wadsworth Center, Empire State Plaza, Albany, NY 12201 0509, USA
    J Mol Biol 374:1345-58. 2007
    ....
  19. pmc Structures of modified eEF2 80S ribosome complexes reveal the role of GTP hydrolysis in translocation
    Derek J Taylor
    Howard Hughes Medical Institute, Health Research Inc, Wadsworth Center, Albany, NY 12201 0509, USA
    EMBO J 26:2421-31. 2007
    ..GTP hydrolysis then uncouples the mRNA-tRNA complex from the decoding center so translocation of the mRNA-tRNA moiety may be completed by a head rotation of the small subunit...
  20. pmc Visualizing the transfer-messenger RNA as the ribosome resumes translation
    Jie Fu
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA
    EMBO J 29:3819-25. 2010
    ....
  21. ncbi request reprint Cryo-EM visualization of a viral internal ribosome entry site bound to human ribosomes: the IRES functions as an RNA-based translation factor
    Christian M T Spahn
    Howard Hughes Medical Institute, Health Research Inc at, Albany, NY 10012, USA
    Cell 118:465-75. 2004
    ..Conformational changes in the ribosome as well as within the IRES itself show that CrPV IRES actively manipulates the ribosome. CrPV-like IRES elements seem to act as RNA-based translation factors...
  22. pmc High-resolution cryo-electron microscopy structure of the Trypanosoma brucei ribosome
    Yaser Hashem
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA
    Nature 494:385-9. 2013
    ....
  23. pmc Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation process
    Mikel Valle
    Howard Hughes Medical Institute, Health Research, Inc, Empire State Plaza, Albany, NY 12201 0509, USA
    EMBO J 21:3557-67. 2002
    ....
  24. pmc Molecular dynamics of EF-G during translocation
    Wen Li
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA
    Proteins 79:1478-86. 2011
    ....
  25. ncbi request reprint The cryo-EM structure of a translation initiation complex from Escherichia coli
    Gregory S Allen
    Howard Hughes Medical Institute, Health Research, Inc at the Wadsworth Center, Albany, New York 12201, USA
    Cell 121:703-12. 2005
    ..Additionally, we present evidence for the localization of IF1, IF3, one C-terminal domain of L7/L12, and the N-terminal domain of IF2 in the initiation complex...
  26. pmc Mechanism of tetracycline resistance by ribosomal protection protein Tet(O)
    Wen Li
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA
    Nat Commun 4:1477. 2013
    ..On the basis of these findings, the mechanism of Tet(O)-mediated tetracycline resistance can be explained in molecular detail...
  27. pmc Visualization of the hybrid state of tRNA binding promoted by spontaneous ratcheting of the ribosome
    Xabier Agirrezabala
    Howard Hughes Medical Institute, Columbia University, 630 West 168th Street, College of Physicians and Surgeons, Black Building 2 221, New York, NY 10032, USA
    Mol Cell 32:190-7. 2008
    ..In addition, we show how the repositioning of the tRNAs goes hand in hand with the change in the interplay between S13, L1 stalk, L5, H68, H69, and H38 that is caused by the ratcheting of the small subunit...
  28. pmc Structure of the mammalian ribosomal 43S preinitiation complex bound to the scanning factor DHX29
    Yaser Hashem
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Cell 153:1108-19. 2013
    ..The structure provides insights into eukaryote-specific aspects of translation, including the mechanism of action of DHX29...
  29. doi request reprint Affinity grid-based cryo-EM of PKC binding to RACK1 on the ribosome
    Gyanesh Sharma
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York City, NY 10032, USA
    J Struct Biol 181:190-4. 2013
    ..This study is a first step in understanding the molecular framework of PKC βII/RACK1 interaction and its role in translation...
  30. ncbi request reprint A cryo-electron microscopic study of ribosome-bound termination factor RF2
    Urmila B S Rawat
    Howard Hughes Medical Institute, Health Research, Inc, Empire State Plaza, Albany NY 12201 0509, USA
    Nature 421:87-90. 2003
    ..The results indicate new interpretations of accuracy in termination, and have implications for how the presence of a stop codon in the DC is signalled to PTC...
  31. pmc Elongation in translation as a dynamic interaction among the ribosome, tRNA, and elongation factors EF-G and EF-Tu
    Xabier Agirrezabala
    The Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA
    Q Rev Biophys 42:159-200. 2009
    ....
  32. ncbi request reprint Ribosome dynamics: insights from atomic structure modeling into cryo-electron microscopy maps
    Kakoli Mitra
    Howard Hughes Medical Institute, Wadsworth Center, Empire State Plaza, Albany, New York 12201 0509, USA
    Annu Rev Biophys Biomol Struct 35:299-317. 2006
    ..Interpretation of cryo-EM reconstructions of the ribosome in quasi-atomic detail reveals a picture in which the ribosome uses RNA not only to catalyze chemical reactions, but also as a means for signal transduction over large distances...
  33. pmc Structure of the E. coli protein-conducting channel bound to a translating ribosome
    Kakoli Mitra
    Howard Hughes Medical Institute, Health Research Inc at the Wadsworth Center, Empire State Plaza, Albany, New York 12201 0509, USA
    Nature 438:318-24. 2005
    ..On the basis of our observation in the translocating PCC of two segregated pores with different degrees of access to bulk lipid, we propose a model for co-translational protein translocation...
  34. pmc Cryo-EM visualization of the ribosome in termination complex with apo-RF3 and RF1
    Jesper Pallesen
    Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University, New York City, United States
    elife 2:e00411. 2013
    ..In conjunction with previous observations, our data provide the foundation to structurally characterize the complete action cycle of the G-protein RF3. DOI:http://dx.doi.org/10.7554/eLife.00411.001...
  35. pmc Visualization of macromolecular complexes using cryo-electron microscopy with FEI Tecnai transmission electron microscopes
    Robert A Grassucci
    Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University College of Physicians and Surgeons, 630 West 168th Street, New York, New York 10032, USA
    Nat Protoc 3:330-9. 2008
    ..These options include microscope settings, choice of detectors and data collection strategies both in situations where a 3D reference is available and in the absence of such a reference (random-conical and common lines)...
  36. ncbi request reprint Electron microscopy of functional ribosome complexes
    Joachim Frank
    Howard Hughes Medical Institute, Health Research, Inc, at the Wadsworth Center, and Department of Biomedical Sciences, State University of New York at Albany, Empire State Plaza, Albany, NY 12201 0509, USA
    Biopolymers 68:223-33. 2003
    ..This review follows the events of the functional cycle and discusses the findings in the context provided by the recently published x-ray structures...
  37. ncbi request reprint A model for co-translational translocation: ribosome-regulated nascent polypeptide translocation at the protein-conducting channel
    Kakoli Mitra
    Howard Hughes Medical Institute, Health Research, Inc at the Wadsworth Center, Empire State Plaza, Albany, NY 12201 0509, USA
    FEBS Lett 580:3353-60. 2006
    ..Our hypothesis suggests a plausible mechanism for the transitioning of the PCC between different configurations...
  38. pmc The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components
    Haixiao Gao
    Howard Hughes Medical Institute, Health Research, Inc, at the Wadsworth Center, Empire State Plaza, Albany, NY 12201 0509, USA
    Proc Natl Acad Sci U S A 102:10206-11. 2005
    ..We propose that this rRNA structure likely participates in the recruitment of ribosome onto the 5' end of mRNA, in facilitating and modulating the initiation of translation that is unique to the trypanosomes...
  39. pmc Reference-free particle selection enhanced with semi-supervised machine learning for cryo-electron microscopy
    Robert Langlois
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    J Struct Biol 175:353-61. 2011
    ..We propose a reference-free method to quickly extract particles from the micrograph. The method is augmented with a new semi-supervised machine-learning algorithm to accurately discriminate particles from contaminants and noise...
  40. ncbi request reprint A cold look at transcription
    Joachim Frank
    Howard Hughes Medical Institute, Health Research, Inc, Wadsworth Center, Empire State Plaza, Albany, NY 12201, USA
    Structure 10:1156-7. 2002
  41. ncbi request reprint Structural insights on the translation initiation complex: ghosts of a universal initiation complex
    Gregory S Allen
    Howard Hughes Medical Institute, Health Research, Inc, Wadsworth Center, Empire State Plaza, Albany, NY 12201, USA
    Mol Microbiol 63:941-50. 2007
    ....
  42. pmc Definition and estimation of resolution in single-particle reconstructions
    Hstau Y Liao
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Structure 18:768-75. 2010
    ..Finally, the drawbacks of resolution estimation are entirely avoided by computing the correlation of neighboring voxels in the Fourier domain...
  43. pmc Preparation of macromolecular complexes for cryo-electron microscopy
    Robert A Grassucci
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons, 630 West 168th Street, New York, New York 10032, USA
    Nat Protoc 2:3239-46. 2007
    ..A companion protocol details how to collect cryo-EM data using an FEI Tecnai transmission electron microscope that can subsequently be processed to obtain a three-dimensional reconstruction of the macromolecular complex...
  44. ncbi request reprint Molding atomic structures into intermediate-resolution cryo-EM density maps of ribosomal complexes using real-space refinement
    Haixiao Gao
    Howard Hughes Medical Institute, Health Research, Inc at the Wadsworth Center, Empire State Plaza, Albany, New York 12201, USA
    Structure 13:401-6. 2005
    ..The example of the ribosome may provide some guidelines for general applications of real-space refinement to flexible fitting problems...
  45. ncbi request reprint An assay for local quality in cryo-electron micrographs of single particles
    Haixiao Gao
    Howard Hughes Medical Institute, Wadsworth Center, Albany, New York 12201 0509, USA
    Ultramicroscopy 93:169-78. 2002
    ..The method of assaying, resulting in "local quality maps", may be useful as a general tool for screening micrographs used as input for reconstructions...
  46. pmc Toward an understanding of the structural basis of translation
    Joachim Frank
    Howard Hughes Medical Institute, Health Research, Inc, at the Wadsworth Center and Department of Biomedical Sciences, State University of New York at Albany, Empire State Plaza, Albany, NY 12201 0509, USA
    Genome Biol 4:237. 2003
    ....
  47. ncbi request reprint Three-dimensional imaging of biological complexity
    Joachim Frank
    Howard Hughes Medical Institute, Health Research, Incorporated, Albany, NY 12201 0509, USA
    J Struct Biol 138:85-91. 2002
    ..As techniques of cryo-microtomy are perfected, we hope to be able to apply tomography to high-pressure frozen sections of tissue...
  48. ncbi request reprint Single-particle imaging of macromolecules by cryo-electron microscopy
    Joachim Frank
    Howard Hughes Medical Institute, Health Research Inc at the Wadsworth Center, State University of New York at Albany, Empire State Plaza, P O Box 509, Albany, NY 12201 0509, USA
    Annu Rev Biophys Biomol Struct 31:303-19. 2002
    ....
  49. pmc The extracellular architecture of adherens junctions revealed by crystal structures of type I cadherins
    Oliver J Harrison
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA
    Structure 19:244-56. 2011
    ..Our observations are consistent with a model for junction assembly involving strong trans and weak cis interactions localized in the ectodomain...
  50. pmc A clarification of the terms used in comparing semi-automated particle selection algorithms in cryo-EM
    Robert Langlois
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    J Struct Biol 175:348-52. 2011
    ....
  51. ncbi request reprint Time-resolved imaging of macromolecular processes and interactions
    Joachim Frank
    Howard Hughes Medical Institute, Health Research Inc at the Wadsworth Center, Albany, NY, USA
    J Struct Biol 147:209-10. 2004
  52. pmc Molecular architecture of the human pre-mRNA 3' processing complex
    Yongsheng Shi
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    Mol Cell 33:365-76. 2009
    ..Together, our data has revealed the complexity and molecular architecture of the pre-mRNA 3' processing complex...

Research Grants16

  1. STRUCTURAL ANALYSIS OF MACROMOLECULAR ASSEMBLY
    Joachim Frank; Fiscal Year: 2010
    ..To achieve these goals, we collaborate with key experts in the areas of reconstruction algorithms, ribosome biochemistry, X-ray crystallography, and computational modeling. ..
  2. STRUCTURAL ANALYSIS OF MACROMOLECULAR ASSEMBLY
    Joachim Frank; Fiscal Year: 2009
    ..To achieve these goals, we collaborate with key experts in the areas of reconstruction algorithms, ribosome biochemistry, X-ray crystallography, and computational modeling. ..
  3. EXPERIMENTALLY GUIDED RIBOSOMAL RNA MODELING
    Joachim Frank; Fiscal Year: 2009
    ..We believe that the interdisciplinary efforts proposed are likely to advance the understanding of the molecular basis of translation. ..
  4. EXPERIMENTALLY GUIDED RIBOSOMAL RNA MODELING
    Joachim Frank; Fiscal Year: 2004
    ..Willy Wriggers (tools for fitting and docking of atomic structures into cryo-EM density maps). Specific Aim #3 is the large-scale (non-atomic) modeling of the intersubunit ratchet movement during translocation observed by this group. ..
  5. EXPERIMENTALLY GUIDED RIBOSOMAL RNA MODELING
    Joachim Frank; Fiscal Year: 2000
    ..This approach will be extended to the eukaryotic ribosome as cryo data become available. We will also develop interactive tools to aid in the building of 3D medium resolution (10-15 Angstroms) models of ribonucleoprotein complexes. ..
  6. EXPERIMENTALLY GUIDED RIBOSOMAL RNA MODELING
    Joachim Frank; Fiscal Year: 2010
    ..Specifically, understanding the function of bacterial ribosomes is furthering our ability to combat drug resistance. ..