Research Topics
Genomes and Genes | Harold BremSummaryAffiliation: Columbia University Country: USA Publications
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Publications
Protocol for the successful treatment of pressure ulcersHarold Brem
Department of Surgery, Columbia University College of Physicians and Surgeons, 5141 Broadway, New York, New York 10034, USA
Am J Surg 188:9-17. 2004..Availability of the described treatment modalities, in combination with early recognition and regular monitoring, ensures rapid healing and minimizes morbidity, mortality, and costs...- Clinical efficacy and mechanism of bilayered living human skin equivalent (HSE) in treatment of diabetic foot ulcersHarold Brem
Angiogenesis and Wound Healing Laboratory, Mount Sinai Medical Center, New York, NY, USA
Surg Technol Int 11:23-31. 2003..We hypothesize that bilayered HSE generates its effect by way of the local synthesis and release of multiple growth factors in specific combination and concentration, which improves the impaired reparative process of chronic wounds... - Healing of elderly patients with diabetic foot ulcers, venous stasis ulcers, and pressure ulcersHarold Brem
Wound Healing Program, Angiogenesis and Wound Healing Laboratory, The Mount Sinai Medical Center, New York, New York
Surg Technol Int 11:161-7. 2003..We hypothesize that if all elderly patients with chronic wounds are provided early treatment, morbidities (e.g., amputation, sepsis, pain) and associated costs will decrease... - Protocol for the successful treatment of venous ulcersHarold Brem
Department of Surgery, Columbia University College of Physicians and Surgeons, 5141 Broadway, New York, New York 10034, USA
Am J Surg 188:1-8. 2004..These treatment modalities, in combination with early recognition and regular monitoring using digital photography and planimetry measurements, will ensure rapid healing and minimize complications and cost... - Evidence-based protocol for diabetic foot ulcersHarold Brem
Department of Surgery, Wound Healing Program, Columbia University College of Physicians and Surgeons, New York, NY, USA
Plast Reconstr Surg 117:193S-209S; discussion 210S-211S. 2006..Strict adherence to evidence-based protocols as described herein will prevent the majority of these amputations... 
Cellular and molecular basis of wound healing in diabetesHarold Brem
Wound Healing and Vascular Biology Laboratory, Division of Plastic Surgery, Columbia University College of Physicians and Surgeons, 5141 Broadway, New York, NY 10034, USA
J Clin Invest 117:1219-22. 2007..Thus, Gallagher et al. have identified novel potential targets for therapeutic intervention in diabetic wound healing...- The synergism of age and db/db genotype impairs wound healingHarold Brem
Department of Surgery, Columbia University College of Physicians and Surgeons, New York, NY, USA
Exp Gerontol 42:523-31. 2007.... - Molecular markers in patients with chronic wounds to guide surgical debridementHarold Brem
Columbia University College of Physicians and Surgeons, Department of Surgery, Wound Healing and Vascular Biology Laboratory, New York, NY, USA
Mol Med 13:30-9. 2007..In the future, molecular markers will be developed to identify the nonimpaired tissue, thereby making surgical debridement more accurate and more efficacious... 
Deregulation of keratinocyte differentiation and activation: a hallmark of venous ulcersOlivera Stojadinovic
Hospital for Special Surgery of the Weill Cornell Medical College, Tissue Repair Lab, New York, NY 10021, USA
J Cell Mol Med 12:2675-90. 2008..We conclude that keratinocytes at the non-healing edges of venous ulcers do not execute either activation or differentiation pathway, resulting in thick callus-like formation at the edge of a venous ulcers...- Novel genomic effects of glucocorticoids in epidermal keratinocytes: inhibition of apoptosis, interferon-gamma pathway, and wound healing along with promotion of terminal differentiationOlivera Stojadinovic
Hospital for Special Surgery, Tissue Repair Laboratory, Tissue Engineering, Regeneration and Repair Program, New York, New York 10021, USA
J Biol Chem 282:4021-34. 2007..These results provide new insights into the beneficial and adverse effects of GCs in the epidermis, defining the participating genes and mechanisms that coordinate the cellular responses important for GC-based therapies... - Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSFHarold Brem
Department of Surgery, Division of Wound Healing and Regenerative Medicine, New York University School of Medicine, New York, NY, USA
J Transl Med 6:75. 2008..2) To describe a methodology to create fibroblast cell lines from patients with chronic wounds... - Mechanism of sustained release of vascular endothelial growth factor in accelerating experimental diabetic healingHarold Brem
Helen and Martin Kimmel Wound Center, Division of Wound Healing and Regenerative Medicine, Department of Surgery, New York University, New York, New York 10016, USA
J Invest Dermatol 129:2275-87. 2009..In the future, local administration and sustained, controlled release of VEGF(165) may decrease amputations in patients with diabetic foot ulcers and possibly accelerate closure of venous ulcers and pressure ulcers... - Molecular pathogenesis of chronic wounds: the role of beta-catenin and c-myc in the inhibition of epithelialization and wound healingOlivera Stojadinovic
The Ronald O Perelman Department of Dermatology, New York University School of Medicine, 550 First Ave, TH100, New York, NY 10016, USA
Am J Pathol 167:59-69. 2005..The presence of activated beta-catenin and c-myc in the epidermis of chronic wounds may serve as a molecular marker of impaired healing and may provide future targets for therapeutic intervention... - Wound-healing protocols for diabetic foot and pressure ulcersHarold Brem
Wound Healing Program, Angiogenesis and Wound Healing Laboratory, The Mount Sinai Medical Center, New York, NY, USA
Surg Technol Int 11:85-92. 2003..If this paradigm is followed, most diabetic foot and pressure ulcers are expected to heal... - Using gene transcription patterns (bar coding scans) to guide wound debridement and healingMarjana Tomic-Canic
Tissue Repair Laboratory, Tissue Engineering Regeneration and Repair Program, Weill Cornell Medical College, New York, NY, USA
Adv Skin Wound Care 21:487-92; quiz 493-4. 2008..Explain the role of keratinocytes in wound healing. 2. Discuss new research findings on the physiological differences between healing and nonhealing wounds.3. Identify implications of the new research for debridement of chronic wounds... - Protocol for treatment of diabetic foot ulcersHarold Brem
Department of Surgery, Columbia University College of Physicians and Surgeons, 5141 Broadway, New York, New York 10034, USA
Am J Surg 187:1S-10S. 2004.... - Surgical pathology to describe the clinical margin of debridement of chronic wounds using a wound electronic medical recordMichael S Golinko
Helen and Martin Kimmel Wound Center, Division of Wound Healing and Regenerative Medicine, Department of Surgery, New York University School of Medicine, New York, NY 10016, USA
J Am Coll Surg 209:254-260.e1. 2009..Unlike tumor resection, however, operative wound specimens are not routinely sent for pathology. The objective of this study was to describe the pathology present in chronic wounds... - Cortisol synthesis in epidermis is induced by IL-1 and tissue injurySasa Vukelic
Department of Medicine, Lenox Hill Hospital, New York, New York 10075, USA
J Biol Chem 286:10265-75. 2011..Inhibition of GC synthesis accelerated wound closure in vivo, providing the evidence that modulation of cortisol synthesis in epidermis may be an important regulatory mechanism during wound healing... - ADAM12: a potential target for the treatment of chronic woundsAsheesh Harsha
Arthritis and Tissue Degeneration Program, Hospital for Special Surgery at Weill Medical College of Cornell University, New York, NY, USA
J Mol Med (Berl) 86:961-9. 2008.... - Prevention and reversal of diabetic nephropathy in db/db mice treated with alagebrium (ALT-711)Melpomeni Peppa
Department of Geriatrics, Mount Sinai School of Medicine, New York, NY, USA
Am J Nephrol 26:430-6. 2006..Alagebrium (ALT-711) has been shown to improve renal dysfunction in animal models of diabetes... - Growth factors and cytokines in wound healingStephan Barrientos
University of Rochester School of Medicine and Dentistry, Rochester, NY, USA
Wound Repair Regen 16:585-601. 2008..This review will focus on the specific roles of these growth factors and cytokines during the wound healing process... - Pathogenesis and treatment of pain in patients with chronic woundsGordon Freedman
Division of Pain Medicine/Department of Anesthesiology, Mount Sinai Medical Center, New York, NY, USA
Surg Technol Int 11:168-79. 2003..The expectation is that all pain should be resolved in all patients if both the wound-healing and pain-healthcare providers use current technologies and drugs... - Wound emergencies: the importance of assessment, documentation, and early treatment using a wound electronic medical recordMichael S Golinko
Helen and Martin Kimmel Wound Center, Department of Surgery, New York University School of Medicine, 301 East 17th Street, New York, NY 10003, USA
Ostomy Wound Manage 55:54-61. 2009..Future research is needed utilizing the WEMR across multiple medical centers to further define criteria for a chronic wound emergency... - High cost of stage IV pressure ulcersHarold Brem
Division of Wound Healing and Regenerative Medicine, Helen and Martin Kimmel Wound Center, Department of Surgery, New York University School of Medicine, New York, NY, USA
Am J Surg 200:473-7. 2010..The aim of this study was to calculate and analyze the cost of treatment for stage IV pressure ulcers... - Practical treatment of pain in patients with chronic wounds: pathogenesis-guided managementGordon Freedman
Department of Anesthesiology, Mount Sinai Pain Management Service, 5 East 98th Street, Box 1192, Mount Sinai School of Medicine, New York, New York 10029 6574, USA
Am J Surg 188:31-5. 2004..By following this protocol, the wound-healing clinician can expect decreased length of hospital stay and resolution of pain in nearly all patients with wounds... - Preliminary development of a diabetic foot ulcer database from a wound electronic medical record: a tool to decrease limb amputationsMichael S Golinko
Department of Surgery, Division of Wound Healing and Regenerative Medicine, Helen and Martin Kimmel Wound Center, New York University School of Medicine, 301 East 17th Street, New York, NY 10003, USA
Wound Repair Regen 17:657-65. 2009..This report describes a method of capturing data relevant to clinical care of a patient with a diabetic foot ulcer, and may enable clinicians to adapt such a system to their own patient population... - Adverse effects of dietary glycotoxins on wound healing in genetically diabetic miceMelpomeni Peppa
Division of Experimental Diabetes and Aging, Department of Geriatrics, Mount Sinai School of Medicine, New York, New York 10029, USA
Diabetes 52:2805-13. 2003..Thus, increased diet-derived AGE intake may be a significant retardant of wound closure in diabetic mice; dietary AGE restriction may improve impaired diabetic wound healing... - The role of vascular endothelial growth factor in wound healingPhilip Bao
University of Pittsburgh, Department of Surgery, Pittsburgh, Pennsylvania, USA
J Surg Res 153:347-58. 2009..The objective is to review is the multiple mechanisms of vascular endothelial growth factor (VEGF) in wound healing... - Gene array technology and pathogenesis of chronic woundsMarjana Tomic-Canic
Department of Dermatology, New York University School of Medicine, 550 First Avenue, MSB 234, New York, New York 10016, USA
Am J Surg 188:67-72. 2004.... - The book of opposites: the role of the nuclear receptor co-regulators in the suppression of epidermal genes by retinoic acid and thyroid hormone receptorsSang H Jho
The Ronald O. Perelman Department of Dermatology, New York University School of Medicine, New York, New York 10016, USA
J Invest Dermatol 124:1034-43. 2005..This novel molecular mechanism of transcriptional repression conveys how RA and T3 target specific groups of epidermal genes, thus exerting intrinsic tissue specificity... - From an enhanceosome to a repressosome: molecular antagonism between glucocorticoids and EGF leads to inhibition of wound healingBrian Lee
New York University School of Medicine, The Ronald O. Perelman Department of Dermatology, 550 First Avenue, New York, NY 10016, USA
J Mol Biol 345:1083-97. 2005..Similar to clinical findings, pre-treatment of keratinocytes with RA blocks GC-mediated inhibition. In summary, our results identify complex molecular mechanisms through which RA alleviates GC-mediated inhibition of wound healing... - Regional anaesthesia with sedation protocol to safely debride sacral pressure ulcersDaniel K O'Neill
Department of Anesthesiology, New York University School of Medicine, New York, NY 10016, USA
Int Wound J 9:525-43. 2012..We are functioning under the hypothesis that regional anaesthesia has advantages over general anaesthesia. We describe our regional anaesthesia protocol for perioperative and postoperative management... - Pyoderma gangrenosum versus chronic venous ulceration: comparison of diagnostic featuresKathie Koo
Department of Dermatology, Mount Sinai School of Medicine of New York University, New York, USA
J Cutan Med Surg 10:26-30. 2006..To confirm the diagnosis, specific features should be sought, including pathergy, crater-like holes or cribriform scarring, and association with inflammatory bowel disease. Other causes of ulceration should be excluded... - Statistical analysis of wound-healing rates for pressure ulcersSylvan Wallenstein
Department of Biomathematical Sciences, 1 Gustave Levy Place, Box 1023, Mount Sinai School of Medicine, New York, New York 10029, USA
Am J Surg 188:73-8. 2004..This approach yields a model useful for identifying prognostic factors, evaluating treatments, and improving our understanding of the variables that affect the wound-healing process... - Cultured bilayered skin allograft for vaginal constructionAlbert Altchek
Department of Obstetrics, Gynecology and Reproductive Science, Mount Sinai School of Medicine and Hospital, New York, NY, USA
J Pediatr Adolesc Gynecol 23:e5-8. 2010..This is the first report of live human cultured bilayered skin allograft (taken from another person) (LHCBSA) to line a dissected space to create a vagina... - Pressure ulcers in the chronically critically ill patientHarold Brem
Department of Surgery and Medicine, Mount Sinai School of Medicine, One Gustav L Levy Place, New York, NY 10029, USA
Crit Care Clin 18:683-94. 2002..Treatment decisions should be made within the context of the patient's overall care goals... - Guidelines for the prevention of diabetic ulcersDavid L Steed
University of Pittsburgh/UPMC, Pittsburgh, Pennsylvania, USA
Wound Repair Regen 16:169-74. 2008 - Development of a severity scale for evaluating the need for Graftskin in nonhealing venous ulcersMorris D Kerstein
Veterans Administration Hospital, Wilmington, DE, USA
Adv Skin Wound Care 15:66-71. 2002..To optimize the severity scale, future trials should incorporate a method to review the interaction of known factors that impair wound healing...
Research Grants
- Local Angiogenic Gene Therapy/VEGF/Treat Diabetic UlcersHarold Brem; Fiscal Year: 2003..In addition, we will analyze the wound fluid in order to determine the temporal expression of VEGF in a diabetic ulcer. ..
- Local Angiogenic Therapy for Diabetic UlcersHarold Brem; Fiscal Year: 2006..Support from this proposal will allow the applicant to use knowledge from the fields of gene therapy, angiogenesis and wound healing to develop a safe angiogenic gene therapy for diabetic foot ulcers. ..