Research Topics
Genomes and GenesSpecies | Carol J WiluszSummaryAffiliation: Colorado State University Country: USA Publications
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Publications
Bringing the role of mRNA decay in the control of gene expression into focusCarol J Wilusz
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
Trends Genet 20:491-7. 2004..These and other aspects of the field are discussed...- Eukaryotic Lsm proteins: lessons from bacteriaCarol J Wilusz
Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523, USA
Nat Struct Mol Biol 12:1031-6. 2005..In this perspective, we have highlighted several functions that Hfq shares with Lsm proteins and put forward hypotheses based on parallels between the two that might further the understanding of Lsm function... 
Sindbis virus usurps the cellular HuR protein to stabilize its transcripts and promote productive infections in mammalian and mosquito cellsKevin J Sokoloski
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
Cell Host Microbe 8:196-207. 2010..These data indicate that Sindbis virus and likely other alphaviruses usurp the HuR protein to avoid the cellular mRNA decay machinery and maintain a highly productive infection...- Dephosphorylation of HuR protein during alphavirus infection is associated with HuR relocalization to the cytoplasmAlexa M Dickson
Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
J Biol Chem 287:36229-38. 2012..Collectively, these data indicate that virus-induced HuR relocalization to the cytoplasm is specific to alphavirus infections and is associated with distinct posttranslational modifications of this RNA-binding protein... - The RNA-binding protein CUGBP1 regulates stability of tumor necrosis factor mRNA in muscle cells: implications for myotonic dystrophyLibin Zhang
Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
J Biol Chem 283:22457-63. 2008..Importantly, overexpression of this potent cytokine could contribute to the muscle wasting and insulin resistance that are characteristic of this debilitating disease... - The PARN deadenylase targets a discrete set of mRNAs for decay and regulates cell motility in mouse myoblastsJerome E Lee
Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, United States of America
PLoS Genet 8:e1002901. 2012..Collectively, these data indicate that PARN modulates decay of a defined set of mRNAs in mammalian cells and implicate this deadenylase in coordinating control of genes required for cell movement... - Systematic analysis of cis-elements in unstable mRNAs demonstrates that CUGBP1 is a key regulator of mRNA decay in muscle cellsJerome E Lee
Program in Cell and Molecular Biology, Colorado State University, Fort Collins, Colorado, USA
PLoS ONE 5:e11201. 2010..Moreover, just as transcription factor activity varies between different cell types, the factors influencing mRNA decay are also cell-type specific... - Nucleophosmin deposition during mRNA 3' end processing influences poly(A) tail lengthFumihiko Sagawa
Cell and Molecular Biology Graduate Program, Colorado State University, Fort Collins, CO, USA
EMBO J 30:3994-4005. 2011..Collectively, these data suggest that NPM1 has an important role in poly(A) tail length determination and may help network 3' end processing with other aspects of nuclear mRNA maturation... - A noncoding RNA produced by arthropod-borne flaviviruses inhibits the cellular exoribonuclease XRN1 and alters host mRNA stabilityStephanie L Moon
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
RNA 18:2029-40. 2012..We conclude that flaviviruses incapacitate XRN1 during infection and dysregulate host mRNA stability as a result of sfRNA formation... 
Chapter 5. In vivo analysis of the decay of transcripts generated by cytoplasmic RNA virusesNicole L Garneau
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA
Methods Enzymol 449:97-123. 2008..Additionally, a hybrid selection protocol for identifying viral-specific small RNAs that are generated during infection by the RNAi branch of the cellular RNA decay machinery is described...- The 3' untranslated region of sindbis virus represses deadenylation of viral transcripts in mosquito and Mammalian cellsNicole L Garneau
Colorado State University, Department of Microbiology, Immunology and Pathology, 1682 Campus Delivery, Fort Collins, CO 80523 1682, USA
J Virol 82:880-92. 2008.... - Global analysis reveals multiple pathways for unique regulation of mRNA decay in induced pluripotent stem cellsAshley T Neff
Program in Cell and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523, USA
Genome Res 22:1457-67. 2012..Overall, our results highlight the importance of post-transcriptional control in pluripotent cells and identify miRNAs and RNA-binding proteins whose activity may coordinately control expression of a wide range of genes in iPS cells... - Lsm proteins bind and stabilize RNAs containing 5' poly(A) tractsNaomi Bergman
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80525, USA
Nat Struct Mol Biol 14:824-31. 2007..We propose that the Lsm complex simultaneously binds the 3' and 5' ends of these unusual messenger RNAs and thereby prevents 3'-to-5' decay. The implications of this phenomenon for cellular mRNA decay are discussed... - The preparation and applications of cytoplasmic extracts from mammalian cells for studying aspects of mRNA decayKevin J Sokoloski
Department of Microbiology, Immunology and Pathology, Colorado State University, Ft Collins, Colorado, USA
Methods Enzymol 448:139-63. 2008..Collectively these procedures represent an important tool for the mechanistic analysis of RNA decay in mammalian cells... - Sequence-specific RNA binding mediated by the RNase PH domain of components of the exosomeJohn R Anderson
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
RNA 12:1810-6. 2006..These data demonstrate that RNase PH domains in general have an affinity for U- and AU-rich sequences, and broaden the potential role in RNA biology of proteins containing these domains... - Nucleophosmin is selectively deposited on mRNA during polyadenylationViswanathan Palaniswamy
Department of Microbiology, Immunology and Pathology, Colorado State University, 1619 Campus Delivery, Fort Collins, Colorado 80523, USA
Nat Struct Mol Biol 13:429-35. 2006..NPM, therefore, represents a mark left on transcripts as a result of 3'-end processing and may have a role in one or more of a variety of post-transcriptional processes influenced by the polyadenylation event... - HuR-SIRT: the hairy world of posttranscriptional controlCarol J Wilusz
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
Mol Cell 25:485-7. 2007..In this issue of Molecular Cell, demonstrate that the RNA binding protein HuR selectively up- or downregulates the stability of the SIRT1 mRNA (which encodes an important regulatory deacetylase) depending upon phosphorylation by Chk2... - Repeat expansion diseases: when a good RNA turns badAlexa M Dickson
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
Wiley Interdiscip Rev RNA 1:173-92. 2010..In this review, we examine the criteria that influence toxicity of these mutant RNAs and discuss new developments in therapeutic approaches... - New ways to meet your (3') end oligouridylation as a step on the path to destructionCarol J Wilusz
Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
Genes Dev 22:1-7. 2008 - The highways and byways of mRNA decayNicole L Garneau
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
Nat Rev Mol Cell Biol 8:113-26. 2007..In this article, we discuss some of the mechanisms that are used by the cell to mediate and regulate this intriguing process... - RNA recognition by 3'-to-5' exonucleases: the substrate perspectiveHend Ibrahim
Colorado State University, Department of Microbiology, Immunology and Pathology, Fort Collins, CO 80525, USA
Biochim Biophys Acta 1779:256-65. 2008.... - The 3' untranslated region of the rabies virus glycoprotein mRNA specifically interacts with cellular PCBP2 protein and promotes transcript stabilitySaiprasad Palusa
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America
PLoS ONE 7:e33561. 2012..Therefore, we conclude that PCBP2 specifically and selectively interacts with the rabies virus glycoprotein mRNA and that this interaction may contribute to the post-transcriptional regulation of glycoprotein expression... - CUG-BP binds to RNA substrates and recruits PARN deadenylaseKaren C M Moraes
Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, 80523, USA
RNA 12:1084-91. 2006..CUG-BP, therefore, is the first RNA-binding protein shown to directly recruit a deadenylase to an RNA substrate... - HuR and translation--the missing linc(RNA)Carol J Wilusz
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
Mol Cell 47:495-6. 2012..2012) reveal that HuR influences decay of a long noncoding RNA (lincRNA-p21) that in turn hybridizes to mRNA targets and represses their translation-an indirect, but elegant explanation for the impact of HuR on translation efficiency... - Efficient in vitro amplification of chronic wasting disease PrPRESTimothy D Kurt
Department of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA
J Virol 81:9605-8. 2007..5 x 10(9)-fold amplification after six rounds. Highly efficient in vitro amplification of PrP(CWD) is a significant step toward detection of PrP(CWD) in the body fluids or excreta of CWD-susceptible species... - trans Regulation: do mRNAs have a herd mentality?Carol J Wilusz
Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
Dev Cell 18:333-4. 2010..In this issue of Developmental Cell, Reveal et al. show through analysis of the oskar mRNA in Drosophila embryos that regulatory elements within mRNAs can act in trans to influence the behavior of other mRNA molecules... - Viruses: overturning RNA turnoverKevin J Sokoloski
Colorado State University, Department of Microbiology, Immunology and Pathology, Fort Collins, Colorado 80523-1682, USA
RNA Biol 3:140-4. 2006..This approach gives insights into regulatory mechanisms of cellular mRNA decay, as well as reveals novel ways to influence the outcome of viral infections... - Consequences of mRNA wardrobe malfunctionsCarol J Wilusz
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
Cell 143:863-5. 2010..Franks et al. (2010) now show that mRNP remodeling is required even for the death of an mRNA... - Global analysis of Pub1p targets reveals a coordinate control of gene expression through modulation of binding and stabilityRadharani Duttagupta
Department of Molecular Genetics, Microbiology, and Immunology, UMDNJ Robert Wood Johnson Medical School, 675 Hoes Ln, Piscataway, New Jersey 08854 5627, USA
Mol Cell Biol 25:5499-513. 2005..These results suggest that Pub1p binds to discrete subsets of cellular transcripts and posttranscriptionally regulates their expression at multiple levels... - A yeast homologue of Hsp70, Ssa1p, regulates turnover of the MFA2 transcript through its AU-rich 3' untranslated regionRadharani Duttagupta
Department of Molecular Genetics, Microbiology and Immunology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
Mol Cell Biol 23:2623-32. 2003..Stabilization occurs as a result of slower deadenylation in the ssa1(ts) strain, suggesting that Hsp70 is required for activation of the turnover pathway... - Non-stop decay--a new mRNA surveillance pathwayShobha Vasudevan
Department of Molecular Genetics, Microbiology and Immunology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Bioessays 24:785-8. 2002..The non-stop decay mechanism releases ribosomes stalled at the 3' end of a mRNA and stimulates the exosome to rapidly degrade the transcript...
Research Grants
- Typhoon Trio ImagerCarol Wilusz; Fiscal Year: 2007....