Carol J Wilusz

Summary

Affiliation: Colorado State University
Country: USA

Publications

  1. pmc Lsm proteins and Hfq: Life at the 3' end
    Carol J Wilusz
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA
    RNA Biol 10:592-601. 2013
  2. ncbi request reprint Bringing the role of mRNA decay in the control of gene expression into focus
    Carol J Wilusz
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Trends Genet 20:491-7. 2004
  3. ncbi request reprint Eukaryotic Lsm proteins: lessons from bacteria
    Carol J Wilusz
    Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523, USA
    Nat Struct Mol Biol 12:1031-6. 2005
  4. pmc Sindbis virus usurps the cellular HuR protein to stabilize its transcripts and promote productive infections in mammalian and mosquito cells
    Kevin J Sokoloski
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
    Cell Host Microbe 8:196-207. 2010
  5. pmc Systematic analysis of cis-elements in unstable mRNAs demonstrates that CUGBP1 is a key regulator of mRNA decay in muscle cells
    Jerome E Lee
    Program in Cell and Molecular Biology, Colorado State University, Fort Collins, Colorado, USA
    PLoS ONE 5:e11201. 2010
  6. pmc The 3' untranslated region of sindbis virus represses deadenylation of viral transcripts in mosquito and Mammalian cells
    Nicole L Garneau
    Colorado State University, Department of Microbiology, Immunology and Pathology, 1682 Campus Delivery, Fort Collins, CO 80523 1682, USA
    J Virol 82:880-92. 2008
  7. pmc Dephosphorylation of HuR protein during alphavirus infection is associated with HuR relocalization to the cytoplasm
    Alexa M Dickson
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    J Biol Chem 287:36229-38. 2012
  8. pmc The RNA-binding protein CUGBP1 regulates stability of tumor necrosis factor mRNA in muscle cells: implications for myotonic dystrophy
    Libin Zhang
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    J Biol Chem 283:22457-63. 2008
  9. pmc The PARN deadenylase targets a discrete set of mRNAs for decay and regulates cell motility in mouse myoblasts
    Jerome E Lee
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, United States of America
    PLoS Genet 8:e1002901. 2012
  10. pmc Nucleophosmin deposition during mRNA 3' end processing influences poly(A) tail length
    Fumihiko Sagawa
    Cell and Molecular Biology Graduate Program, Colorado State University, Fort Collins, CO, USA
    EMBO J 30:3994-4005. 2011

Research Grants

  1. Typhoon Trio Imager
    Carol Wilusz; Fiscal Year: 2007

Collaborators

  • Bin Tian
  • Richard A Bowen
  • Surachai Supattapone
  • Glenn C Telling
  • Robert J Schneider
  • Jeffrey Wilusz
  • Kevin J Sokoloski
  • Alexa M Dickson
  • Nicole L Garneau
  • John R Anderson
  • Jerome E Lee
  • Karen C M Moraes
  • Mateusz Opyrchal
  • Michael D Barnhart
  • Stephanie L Moon
  • Ju Youn Lee
  • Hend Ibrahim
  • Radharani Duttagupta
  • Irina Vlasova-St Louis
  • Ashley T Neff
  • Saiprasad Palusa
  • Fumihiko Sagawa
  • Stuart W Peltz
  • Shobha Vasudevan
  • Libin Zhang
  • Naomi Bergman
  • Timothy D Kurt
  • Viswanathan Palaniswamy
  • Alexander W Emch
  • Paul R Bohjanen
  • Christina Ndaluka
  • Yutaro Kumagai
  • Lauren Oko
  • Jarrett Trembly
  • Evanthia Galanis
  • Thomas E Morrison
  • Alexander A Khromykh
  • Shizuo Akira
  • Angela L Morrison
  • Emily L Chaskey
  • C Preston Neff
  • Edward A Hoover
  • Bozidarka Zaric
  • Matthew R Perrott
  • Mark D Zabel
  • Christian Kambach
  • Karthika Muthukumaraswamy
  • Devi Mukherjee
  • Joseph P Dougherty
  • Danny T Khounh
  • Patricia Soteropoulos
  • Ming Ouyang

Detail Information

Publications35

  1. pmc Lsm proteins and Hfq: Life at the 3' end
    Carol J Wilusz
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA
    RNA Biol 10:592-601. 2013
    ..Here, we aim to compare and contrast the roles of these evolutionarily related complexes and to highlight areas for future investigation...
  2. ncbi request reprint Bringing the role of mRNA decay in the control of gene expression into focus
    Carol J Wilusz
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Trends Genet 20:491-7. 2004
    ..These and other aspects of the field are discussed...
  3. ncbi request reprint Eukaryotic Lsm proteins: lessons from bacteria
    Carol J Wilusz
    Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523, USA
    Nat Struct Mol Biol 12:1031-6. 2005
    ..In this perspective, we have highlighted several functions that Hfq shares with Lsm proteins and put forward hypotheses based on parallels between the two that might further the understanding of Lsm function...
  4. pmc Sindbis virus usurps the cellular HuR protein to stabilize its transcripts and promote productive infections in mammalian and mosquito cells
    Kevin J Sokoloski
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
    Cell Host Microbe 8:196-207. 2010
    ..These data indicate that Sindbis virus and likely other alphaviruses usurp the HuR protein to avoid the cellular mRNA decay machinery and maintain a highly productive infection...
  5. pmc Systematic analysis of cis-elements in unstable mRNAs demonstrates that CUGBP1 is a key regulator of mRNA decay in muscle cells
    Jerome E Lee
    Program in Cell and Molecular Biology, Colorado State University, Fort Collins, Colorado, USA
    PLoS ONE 5:e11201. 2010
    ..Moreover, just as transcription factor activity varies between different cell types, the factors influencing mRNA decay are also cell-type specific...
  6. pmc The 3' untranslated region of sindbis virus represses deadenylation of viral transcripts in mosquito and Mammalian cells
    Nicole L Garneau
    Colorado State University, Department of Microbiology, Immunology and Pathology, 1682 Campus Delivery, Fort Collins, CO 80523 1682, USA
    J Virol 82:880-92. 2008
    ....
  7. pmc Dephosphorylation of HuR protein during alphavirus infection is associated with HuR relocalization to the cytoplasm
    Alexa M Dickson
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    J Biol Chem 287:36229-38. 2012
    ..Collectively, these data indicate that virus-induced HuR relocalization to the cytoplasm is specific to alphavirus infections and is associated with distinct posttranslational modifications of this RNA-binding protein...
  8. pmc The RNA-binding protein CUGBP1 regulates stability of tumor necrosis factor mRNA in muscle cells: implications for myotonic dystrophy
    Libin Zhang
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    J Biol Chem 283:22457-63. 2008
    ..Importantly, overexpression of this potent cytokine could contribute to the muscle wasting and insulin resistance that are characteristic of this debilitating disease...
  9. pmc The PARN deadenylase targets a discrete set of mRNAs for decay and regulates cell motility in mouse myoblasts
    Jerome E Lee
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, United States of America
    PLoS Genet 8:e1002901. 2012
    ..Collectively, these data indicate that PARN modulates decay of a defined set of mRNAs in mammalian cells and implicate this deadenylase in coordinating control of genes required for cell movement...
  10. pmc Nucleophosmin deposition during mRNA 3' end processing influences poly(A) tail length
    Fumihiko Sagawa
    Cell and Molecular Biology Graduate Program, Colorado State University, Fort Collins, CO, USA
    EMBO J 30:3994-4005. 2011
    ..Collectively, these data suggest that NPM1 has an important role in poly(A) tail length determination and may help network 3' end processing with other aspects of nuclear mRNA maturation...
  11. ncbi request reprint Lsm proteins bind and stabilize RNAs containing 5' poly(A) tracts
    Naomi Bergman
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80525, USA
    Nat Struct Mol Biol 14:824-31. 2007
    ..We propose that the Lsm complex simultaneously binds the 3' and 5' ends of these unusual messenger RNAs and thereby prevents 3'-to-5' decay. The implications of this phenomenon for cellular mRNA decay are discussed...
  12. pmc A noncoding RNA produced by arthropod-borne flaviviruses inhibits the cellular exoribonuclease XRN1 and alters host mRNA stability
    Stephanie L Moon
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    RNA 18:2029-40. 2012
    ..We conclude that flaviviruses incapacitate XRN1 during infection and dysregulate host mRNA stability as a result of sfRNA formation...
  13. doi request reprint Chapter 5. In vivo analysis of the decay of transcripts generated by cytoplasmic RNA viruses
    Nicole L Garneau
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA
    Methods Enzymol 449:97-123. 2008
    ..Additionally, a hybrid selection protocol for identifying viral-specific small RNAs that are generated during infection by the RNAi branch of the cellular RNA decay machinery is described...
  14. pmc Sequence-specific RNA binding mediated by the RNase PH domain of components of the exosome
    John R Anderson
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    RNA 12:1810-6. 2006
    ..These data demonstrate that RNase PH domains in general have an affinity for U- and AU-rich sequences, and broaden the potential role in RNA biology of proteins containing these domains...
  15. pmc Nucleophosmin is selectively deposited on mRNA during polyadenylation
    Viswanathan Palaniswamy
    Department of Microbiology, Immunology and Pathology, Colorado State University, 1619 Campus Delivery, Fort Collins, Colorado 80523, USA
    Nat Struct Mol Biol 13:429-35. 2006
    ..NPM, therefore, represents a mark left on transcripts as a result of 3'-end processing and may have a role in one or more of a variety of post-transcriptional processes influenced by the polyadenylation event...
  16. pmc Global analysis reveals multiple pathways for unique regulation of mRNA decay in induced pluripotent stem cells
    Ashley T Neff
    Program in Cell and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523, USA
    Genome Res 22:1457-67. 2012
    ..Overall, our results highlight the importance of post-transcriptional control in pluripotent cells and identify miRNAs and RNA-binding proteins whose activity may coordinately control expression of a wide range of genes in iPS cells...
  17. doi request reprint The preparation and applications of cytoplasmic extracts from mammalian cells for studying aspects of mRNA decay
    Kevin J Sokoloski
    Department of Microbiology, Immunology and Pathology, Colorado State University, Ft Collins, Colorado, USA
    Methods Enzymol 448:139-63. 2008
    ..Collectively these procedures represent an important tool for the mechanistic analysis of RNA decay in mammalian cells...
  18. pmc New ways to meet your (3') end oligouridylation as a step on the path to destruction
    Carol J Wilusz
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Genes Dev 22:1-7. 2008
  19. ncbi request reprint HuR-SIRT: the hairy world of posttranscriptional control
    Carol J Wilusz
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Mol Cell 25:485-7. 2007
    ..In this issue of Molecular Cell, demonstrate that the RNA binding protein HuR selectively up- or downregulates the stability of the SIRT1 mRNA (which encodes an important regulatory deacetylase) depending upon phosphorylation by Chk2...
  20. pmc CELFish ways to modulate mRNA decay
    Irina Vlasova-St Louis
    Department of Microbiology, University of Minnesota, Minneapolis, MN, USA
    Biochim Biophys Acta 1829:695-707. 2013
    ..We conclude by highlighting the priorities for new experiments in this field. This article is part of a Special Issue entitled: RNA Decay mechanisms...
  21. doi request reprint Repeat expansion diseases: when a good RNA turns bad
    Alexa M Dickson
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Wiley Interdiscip Rev RNA 1:173-92. 2010
    ..In this review, we examine the criteria that influence toxicity of these mutant RNAs and discuss new developments in therapeutic approaches...
  22. pmc RNA recognition by 3'-to-5' exonucleases: the substrate perspective
    Hend Ibrahim
    Colorado State University, Department of Microbiology, Immunology and Pathology, Fort Collins, CO 80525, USA
    Biochim Biophys Acta 1779:256-65. 2008
    ....
  23. ncbi request reprint The highways and byways of mRNA decay
    Nicole L Garneau
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Nat Rev Mol Cell Biol 8:113-26. 2007
    ..In this article, we discuss some of the mechanisms that are used by the cell to mediate and regulate this intriguing process...
  24. pmc CUG-BP binds to RNA substrates and recruits PARN deadenylase
    Karen C M Moraes
    Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, 80523, USA
    RNA 12:1084-91. 2006
    ..CUG-BP, therefore, is the first RNA-binding protein shown to directly recruit a deadenylase to an RNA substrate...
  25. pmc Changes in cellular mRNA stability, splicing, and polyadenylation through HuR protein sequestration by a cytoplasmic RNA virus
    Michael D Barnhart
    Cell and Molecular Biology Program, Colorado State University, Fort Collins, CO 80525, USA
    Cell Rep 5:909-17. 2013
    ..These studies suggest a molecular mechanism of virus-host interaction that probably has a significant impact on virus replication, cytopathology, and pathogenesis. ..
  26. pmc The 3' untranslated region of the rabies virus glycoprotein mRNA specifically interacts with cellular PCBP2 protein and promotes transcript stability
    Saiprasad Palusa
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America
    PLoS ONE 7:e33561. 2012
    ..Therefore, we conclude that PCBP2 specifically and selectively interacts with the rabies virus glycoprotein mRNA and that this interaction may contribute to the post-transcriptional regulation of glycoprotein expression...
  27. ncbi request reprint Viruses: overturning RNA turnover
    Kevin J Sokoloski
    Colorado State University, Department of Microbiology, Immunology and Pathology, Fort Collins, Colorado 80523 1682, USA
    RNA Biol 3:140-4. 2006
    ..This approach gives insights into regulatory mechanisms of cellular mRNA decay, as well as reveals novel ways to influence the outcome of viral infections...
  28. ncbi request reprint A cell-free mRNA stability assay reveals conservation of the enzymes and mechanisms of mRNA decay between mosquito and mammalian cell lines
    Mateusz Opyrchal
    Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, 80523, USA
    Insect Biochem Mol Biol 35:1321-34. 2005
    ..Our results suggest a high level of conservation in the factors and pathways of mRNA decay between mosquitoes and humans...
  29. doi request reprint HuR and translation--the missing linc(RNA)
    Carol J Wilusz
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Mol Cell 47:495-6. 2012
    ..2012) reveal that HuR influences decay of a long noncoding RNA (lincRNA-p21) that in turn hybridizes to mRNA targets and represses their translation-an indirect, but elegant explanation for the impact of HuR on translation efficiency...
  30. ncbi request reprint Consequences of mRNA wardrobe malfunctions
    Carol J Wilusz
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Cell 143:863-5. 2010
    ..Franks et al. (2010) now show that mRNP remodeling is required even for the death of an mRNA...
  31. pmc Efficient in vitro amplification of chronic wasting disease PrPRES
    Timothy D Kurt
    Department of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA
    J Virol 81:9605-8. 2007
    ..5 x 10(9)-fold amplification after six rounds. Highly efficient in vitro amplification of PrP(CWD) is a significant step toward detection of PrP(CWD) in the body fluids or excreta of CWD-susceptible species...
  32. doi request reprint trans Regulation: do mRNAs have a herd mentality?
    Carol J Wilusz
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
    Dev Cell 18:333-4. 2010
    ..In this issue of Developmental Cell, Reveal et al. show through analysis of the oskar mRNA in Drosophila embryos that regulatory elements within mRNAs can act in trans to influence the behavior of other mRNA molecules...
  33. pmc Global analysis of Pub1p targets reveals a coordinate control of gene expression through modulation of binding and stability
    Radharani Duttagupta
    Department of Molecular Genetics, Microbiology, and Immunology, UMDNJ Robert Wood Johnson Medical School, 675 Hoes Ln, Piscataway, New Jersey 08854 5627, USA
    Mol Cell Biol 25:5499-513. 2005
    ..These results suggest that Pub1p binds to discrete subsets of cellular transcripts and posttranscriptionally regulates their expression at multiple levels...
  34. pmc A yeast homologue of Hsp70, Ssa1p, regulates turnover of the MFA2 transcript through its AU-rich 3' untranslated region
    Radharani Duttagupta
    Department of Molecular Genetics, Microbiology and Immunology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Mol Cell Biol 23:2623-32. 2003
    ..Stabilization occurs as a result of slower deadenylation in the ssa1(ts) strain, suggesting that Hsp70 is required for activation of the turnover pathway...
  35. ncbi request reprint Non-stop decay--a new mRNA surveillance pathway
    Shobha Vasudevan
    Department of Molecular Genetics, Microbiology and Immunology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Bioessays 24:785-8. 2002
    ..The non-stop decay mechanism releases ribosomes stalled at the 3' end of a mRNA and stimulates the exosome to rapidly degrade the transcript...

Research Grants1

  1. Typhoon Trio Imager
    Carol Wilusz; Fiscal Year: 2007
    ....