John S Spencer

Summary

Affiliation: Colorado State University
Country: USA

Publications

  1. ncbi Identification of serological biomarkers of infection, disease progression and treatment efficacy for leprosy
    John S Spencer
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA
    Mem Inst Oswaldo Cruz 107:79-89. 2012
  2. ncbi The role of Mycobacterium leprae phenolic glycolipid I (PGL-I) in serodiagnosis and in the pathogenesis of leprosy
    John S Spencer
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
    Lepr Rev 82:344-57. 2011
  3. ncbi Analysis of antibody responses to Mycobacterium leprae phenolic glycolipid I, lipoarabinomannan, and recombinant proteins to define disease subtype-specific antigenic profiles in leprosy
    John S Spencer
    Department of Microbiology, Immunology and Pathology, Colorado State University, Campus Delivery 1682, Fort Collins, CO 80523 1682, USA
    Clin Vaccine Immunol 18:260-7. 2011
  4. ncbi Antigenic specificity of the Mycobacterium leprae homologue of ESAT-6
    John S Spencer
    Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523 1677, USA
    Infect Immun 70:1010-3. 2002
  5. ncbi Identification of specific proteins and peptides in Mycobacterium leprae suitable for the selective diagnosis of leprosy
    John S Spencer
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    J Immunol 175:7930-8. 2005
  6. ncbi Comparative analysis of B- and T-cell epitopes of Mycobacterium leprae and Mycobacterium tuberculosis culture filtrate protein 10
    John S Spencer
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Infect Immun 72:3161-70. 2004
  7. ncbi Deciphering the proteomic profile of Mycobacterium leprae cell envelope
    Maria Angela M Marques
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
    Proteomics 8:2477-91. 2008
  8. ncbi Use of protein microarrays to define the humoral immune response in leprosy patients and identification of disease-state-specific antigenic profiles
    Nathan A Groathouse
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523-1682, USA
    Infect Immun 74:6458-66. 2006
  9. ncbi Continued proteomic analysis of Mycobacterium leprae subcellular fractions
    Maria Angela M Marques
    Department of Microbiology, Colorado State University, Fort Collins, CO 80523-1682, USA
    Proteomics 4:2942-53. 2004
  10. ncbi Identification of amino acids and domains required for catalytic activity of DPPR synthase, a cell wall biosynthetic enzyme of Mycobacterium tuberculosis
    Hairong Huang
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Microbiology 154:736-43. 2008

Collaborators

Detail Information

Publications20

  1. ncbi Identification of serological biomarkers of infection, disease progression and treatment efficacy for leprosy
    John S Spencer
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA
    Mem Inst Oswaldo Cruz 107:79-89. 2012
    ..The results of this study indicate that antibody titres to specific M. leprae antigens can be used to monitor treatment efficacy in LPs and assess disease progression in those most at risk for developing this disease...
  2. ncbi The role of Mycobacterium leprae phenolic glycolipid I (PGL-I) in serodiagnosis and in the pathogenesis of leprosy
    John S Spencer
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
    Lepr Rev 82:344-57. 2011
    ..leprae/leprosy...
  3. ncbi Analysis of antibody responses to Mycobacterium leprae phenolic glycolipid I, lipoarabinomannan, and recombinant proteins to define disease subtype-specific antigenic profiles in leprosy
    John S Spencer
    Department of Microbiology, Immunology and Pathology, Colorado State University, Campus Delivery 1682, Fort Collins, CO 80523 1682, USA
    Clin Vaccine Immunol 18:260-7. 2011
    ..Correlating these response patterns with a particular disease state could allow for a more critical assessment of the form of disease within the leprosy spectrum and could lead to better patient management...
  4. ncbi Antigenic specificity of the Mycobacterium leprae homologue of ESAT-6
    John S Spencer
    Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523 1677, USA
    Infect Immun 70:1010-3. 2002
    ..The protein is expressed in M. leprae and appears in the cell wall fraction. Thus, M. leprae ESAT-6 shows promise as a specific diagnostic agent for leprosy...
  5. ncbi Identification of specific proteins and peptides in Mycobacterium leprae suitable for the selective diagnosis of leprosy
    John S Spencer
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    J Immunol 175:7930-8. 2005
    ..leprae infection and epidemiological surveys of the incidence of leprosy, of which little is known...
  6. ncbi Comparative analysis of B- and T-cell epitopes of Mycobacterium leprae and Mycobacterium tuberculosis culture filtrate protein 10
    John S Spencer
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Infect Immun 72:3161-70. 2004
    ..leprae CFP-10, like its homologue in M. tuberculosis, is a secreted protein...
  7. ncbi Deciphering the proteomic profile of Mycobacterium leprae cell envelope
    Maria Angela M Marques
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
    Proteomics 8:2477-91. 2008
    ..The data presented in this study contribute to our understanding of the in vivo composition and physiology of the mycobacterial cell envelope, a compartment known to play a major role in bacterial pathogenesis...
  8. ncbi Use of protein microarrays to define the humoral immune response in leprosy patients and identification of disease-state-specific antigenic profiles
    Nathan A Groathouse
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523-1682, USA
    Infect Immun 74:6458-66. 2006
    ..leprae protein antigens produced in recombinant form...
  9. ncbi Continued proteomic analysis of Mycobacterium leprae subcellular fractions
    Maria Angela M Marques
    Department of Microbiology, Colorado State University, Fort Collins, CO 80523-1682, USA
    Proteomics 4:2942-53. 2004
    ..Additionally, two highly basic proteins (with pI >10.0) were isolated by heparin affinity chromatography and identified by N-terminal sequencing. This study constitutes the first application of proteomics to a host-derived Mycobacterium...
  10. ncbi Identification of amino acids and domains required for catalytic activity of DPPR synthase, a cell wall biosynthetic enzyme of Mycobacterium tuberculosis
    Hairong Huang
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Microbiology 154:736-43. 2008
    ..Thus, it is plausible that this DPPR synthase has a pRpp binding site that is different from that of the classical eukaryotic enzymes, and further work to develop inhibitors against this enzyme is thereby encouraged...
  11. ncbi A modified synthesis and serological evaluation of neoglycoproteins containing the natural disaccharide of PGL-I from Mycobacterium leprae
    Jian Zhang
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
    Bioorg Med Chem Lett 20:3250-3. 2010
    ..The serological activities of the neoglycoproteins against pooled human lepromatous leprosy sera were measured by ELISA and they were detectable at picogram amounts...
  12. ncbi XCL1 (lymphotactin) chemokine produced by activated CD8 T cells during the chronic stage of infection with Mycobacterium tuberculosis negatively affects production of IFN-gamma by CD4 T cells and participates in granuloma stability
    Diane Ordway
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
    J Leukoc Biol 82:1221-9. 2007
    ..Additionally, we also describe for the first time that during Mtb infection, activated CD8 T cells in the lungs produce XCL1 and that this chemokine is capable of controlling IFN-gamma production by CD4 T cells...
  13. ncbi Glycolytic and non-glycolytic functions of Mycobacterium tuberculosis fructose-1,6-bisphosphate aldolase, an essential enzyme produced by replicating and non-replicating bacilli
    María de la Paz Santangelo
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523 1682, USA
    J Biol Chem 286:40219-31. 2011
    ..Altogether, our results highlight the potential of FBA-tb as a novel therapeutic target against both replicating and non-replicating bacilli...
  14. ncbi Proteomic approaches to antigen discovery
    Karen M Dobos
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, USA
    Methods Mol Med 94:3-17. 2004
    ..As presented or with minor modifications, these techniques may be universally applied to other bacterial pathogens or used to identify bacterial proteins possessing other immunological properties...
  15. ncbi The carboxy terminus of EmbC from Mycobacterium smegmatis mediates chain length extension of the arabinan in lipoarabinomannan
    Libin Shi
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    J Biol Chem 281:19512-26. 2006
    ....
  16. ncbi Gene expression profile and immunological evaluation of unique hypothetical unknown proteins of Mycobacterium leprae by using quantitative real-time PCR
    Hee Jin Kim
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA
    Clin Vaccine Immunol 20:181-90. 2013
    ..leprae-specific immune responses. These nine proteins may be good diagnostic reagents to improve both the sensitivity and specificity of detection of individuals with asymptomatic leprosy...
  17. ncbi Multiple M. tuberculosis phenotypes in mouse and guinea pig lung tissue revealed by a dual-staining approach
    Gavin J Ryan
    Department of Microbiology, Colorado State University, Fort Collins, Colorado, United States of America
    PLoS ONE 5:e11108. 2010
    ..This is relevant information for approaches that study bacillary characteristics in pooled samples (using lipidomics and proteomics) as well as in M. tuberculosis drug development...
  18. ncbi On the origin of leprosy
    Marc Monot
    , Institut Pasteur, Paris, France
    Science 308:1040-2. 2005
    ..The disease seems to have originated in Eastern Africa or the Near East and spread with successive human migrations. Europeans or North Africans introduced leprosy into West Africa and the Americas within the past 500 years...
  19. ncbi Postgenomic approach to identify novel Mycobacterium leprae antigens with potential to improve immunodiagnosis of infection
    Annemieke Geluk
    Department of Immunohematology and Blood Transfusion, LUMC PO Box 9600, 2300 RC Leiden, The Netherlands
    Infect Immun 73:5636-44. 2005
    ..leprae-exposed individuals. These proteins may provide new tools to develop tests for specific diagnosis of M. leprae infection and may enhance our understanding of leprosy and its transmission...
  20. ncbi The level of PPD-specific IFN-gamma-producing CD4+ T cells in the blood predicts the in vivo response to PPD
    Marcia Valéria B S Martins
    Leprosy Laboratory, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil
    Tuberculosis (Edinb) 87:202-11. 2007
    ....