Research Topics
| Herbert SchweizerSummaryAffiliation: Colorado State University Country: USA Publications
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Publications
Antimicrobial drug-selection markers for Burkholderia pseudomallei and B. malleiHerbert P Schweizer
Rocky Mountain Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
Emerg Infect Dis 14:1689-92. 2008..Deliberation and decisions regarding alternative selection markers (antimicrobial and nonantimicrobial drugs) by the international community, regulatory authorities, and funding agencies are needed...
Mechanisms of antibiotic resistance in Burkholderia pseudomallei: implications for treatment of melioidosisHerbert P Schweizer
Colorado State University, Department of Microbiology, Immunology and Pathology, IDRC at Foothills Campus, 0922 Campus Delivery, Fort Collins, CO 80523, USA
Future Microbiol 7:1389-99. 2012..Novel agents and therapeutic strategies are being tested and, in some instances, show promise as anti-B. pseudomallei infectives...- Understanding efflux in Gram-negative bacteria: opportunities for drug discoveryHerbert P Schweizer
Colorado State University, IDRC at Foothills Campus, Department of Microbiology, Immunology and Pathology, Fort Collins, CO 80523 0922, USA
Expert Opin Drug Discov 7:633-42. 2012.... - A simple method for construction of pir+ Enterobacterial hosts for maintenance of R6K replicon plasmidsBrian H Kvitko
Department of Microbiology, Immunology and Pathology, Colorado State University, IDRC at Foothills Campus, 0922 Campus Delivery, Fort Collins, CO 80523, USA
BMC Res Notes 5:157. 2012..In Escherichia coli this is commonly achieved by chromosomal integration of pir either via lysogenization with a λpir phage or homologous recombination at a pre-determined locus... 
An improved method for rapid generation of unmarked Pseudomonas aeruginosa deletion mutantsKyoung-Hee Choi
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA
BMC Microbiol 5:30. 2005..With appropriate modifications, the method should be applicable to other bacteria...- Bacterial genetics: past achievements, present state of the field, and future challengesHerbert Schweizer
Rocky Mountain Regional Center for Biodefense and Emerging Infectious Diseases Research, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1690, USA
Biotechniques 44:633-4, 636-41. 2008..BioTechniques has played an integral part in the publication of important technological advances in the field over the first 25 years of its existence and it can be anticipated that it will continue to do so in the future... 
Vectors to express foreign genes and techniques to monitor gene expression in PseudomonadsH P Schweizer
Department of Microbiology, Colorado State University, Fort Collins, CO 80523, USA
Curr Opin Biotechnol 12:439-45. 2001....- Applications of the Saccharomyces cerevisiae Flp-FRT system in bacterial geneticsHerbert P Schweizer
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, 80523 1682, USA
J Mol Microbiol Biotechnol 5:67-77. 2003..On the contrary, in many instances this technology is probably greatly underutilized, especially in gram-positive bacteria... - Efflux as a mechanism of resistance to antimicrobials in Pseudomonas aeruginosa and related bacteria: unanswered questionsHerbert P Schweizer
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682A, USA
Genet Mol Res 2:48-62. 2003..Despite intensive studies of these multidrug efflux systems over the past several years, their precise molecular architectures, their modes of regulation of expression and their natural functions remain largely unknown... - Methods for genetic manipulation of Burkholderia gladioli pathovar cocovenenansNawarat Somprasong
Department of Biotechnology, Faculty of Science, Mahidol University, Ratchathewi, Bangkok, Thailand, 10400
BMC Res Notes 3:308. 2010..Little is known about the regulation of virulence factor and toxin production in BGC, and studies in this bacterium have been hampered by lack of genetic tools... - Triclosan: a widely used biocide and its link to antibioticsH P Schweizer
Department of Microbiology, Colorado State University, Fort Collins, CO 80523 1677, USA
FEMS Microbiol Lett 202:1-7. 2001.... - The MexJK efflux pump of Pseudomonas aeruginosa requires OprM for antibiotic efflux but not for efflux of triclosanRungtip Chuanchuen
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523-1682, USA
J Bacteriol 184:5036-44. 2002..Furthermore, the results confirm that triclosan is an excellent tool for the study of RND multidrug efflux systems and that this popular biocide therefore readily selects mutants which are cross-resistant with antibiotics... - Method for regulated expression of single-copy efflux pump genes in a surrogate Pseudomonas aeruginosa strain: identification of the BpeEF-OprC chloramphenicol and trimethoprim efflux pump of Burkholderia pseudomallei 1026bAyush Kumar
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523-1682, USA
Antimicrob Agents Chemother 50:3460-3. 2006..Using this method, the Burkholderia pseudomallei bpeEF-oprC operon was shown to encode a chloramphenicol and trimethoprim efflux pump... - Substrate-dependent utilization of OprM or OpmH by the Pseudomonas aeruginosa MexJK efflux pumpRungtip Chuanchuen
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
Antimicrob Agents Chemother 49:2133-6. 2005..This is the first report of natural utilization of multiple OMPs by a given resistance nodulation cell division transporter/membrane fusion protein pair... - Benchtop and microcentrifuge preparation of Pseudomonas aeruginosa competent cellsRungtip Chuanchuen
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins 80523-1682A, USA
Biotechniques 33:760, 762-3. 2002 - Functional characterization of MexXY and OpmG in aminoglycoside efflux in Pseudomonas aeruginosaRungtip Chuanchuen
Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand
Southeast Asian J Trop Med Public Health 39:115-22. 2008..Growth rates of wildtype P. aeruginosa and opmG mutant derivatives were not different, indicating that expression of opmG is not essential for P. aeruginosa growth... - Role of the MexXY multidrug efflux pump in moderate aminoglycoside resistance in Pseudomonas aeruginosa isolates from Pseudomonas mastitisRungtip Chuanchuen
Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand
Microbiol Immunol 52:392-8. 2008..Transcription levels of mexY were independent on mutations in mexZ, suggesting the existence of additional regulatory mechanisms other than mexZ... - The BpeAB-OprB efflux pump of Burkholderia pseudomallei 1026b does not play a role in quorum sensing, virulence factor production, or extrusion of aminoglycosides but is a broad-spectrum drug efflux systemTakehiko Mima
Department of Microbiology, Immunology and Pathology, Colorado State University, IDRC at Foothills Campus, Campus Delivery 2025, Fort Collins, CO 80523 2025, USA
Antimicrob Agents Chemother 54:3113-20. 2010..At present, we do not know why our BpeAB-OprB susceptibility and virulence factor expression results with 1026b and its derivatives are different from those previously published for Singapore strain KHW... - In vivo Himar1 transposon mutagenesis of Burkholderia pseudomalleiDrew A Rholl
Department of Microbiology, Immunology and Pathology, Rocky Mountain Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research, Colorado State University, Fort Collins, CO 80523 1690, USA
Appl Environ Microbiol 74:7529-35. 2008..mallei. Our results demonstrate that comprehensive transposon libraries of B. pseudomallei can be generated, providing additional tools for the study of the biology, pathogenesis, and antibiotic resistance of this pathogen... - Genetic tools for select-agent-compliant manipulation of Burkholderia pseudomalleiKyoung Hee Choi
Department of Microbiology, Immunology and Pathology, Rocky Mountain Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research, Colorado State University, Fort Collins, CO 80523 1682, USA
Appl Environ Microbiol 74:1064-75. 2008..The new tools allow routine select-agent-compliant genetic manipulations of B. pseudomallei and other Burkholderia species... - Identification and characterization of TriABC-OpmH, a triclosan efflux pump of Pseudomonas aeruginosa requiring two membrane fusion proteinsTakehiko Mima
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
J Bacteriol 189:7600-9. 2007..TriABC is the fifth RND pump in P. aeruginosa shown to efficiently efflux triclosan, supporting the notion that efflux is the primary mechanism responsible for this bacterium's high intrinsic and acquired triclosan resistance... - Versatile dual-technology system for markerless allele replacement in Burkholderia pseudomalleiCarolina M Lopez
Department of Microbiology, Immunology and Pathology, Colorado State University, IDRC at Foothills Campus, Campus Delivery 2025, Fort Collins, CO 80523 2025, USA
Appl Environ Microbiol 75:6496-503. 2009..pseudomallei strains. Finally, sacB-based counterselection was employed to isolate a defined chromosomal fabD(Ts) allele that causes synthesis of a temperature-sensitive FabD, an essential fatty acid biosynthesis enzyme... - Molecular characterization of MexL, the transcriptional repressor of the mexJK multidrug efflux operon in Pseudomonas aeruginosaRungtip Chuanchuen
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523-1682, USA
Antimicrob Agents Chemother 49:1844-51. 2005..The MexL-protected region contains two inverted GTATTT repeats, and their location in the protected region and overlap with the mexL and mexJ promoter sequences strongly support a role in MexL binding... - A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformationKyoung-Hee Choi
Department of Microbiology, Immunology and Pathology, Colorado State University, 1682 Campus Delivery, Fort Collins, CO 80523, USA
J Microbiol Methods 64:391-7. 2006.... - Expression of resistance-nodulation-cell-division efflux pumps in commonly used Burkholderia pseudomallei strains and clinical isolates from northern AustraliaAyush Kumar
Department of Microbiology, Immunology, and Pathology, Rocky Mountain Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research, Colorado State University, Fort Collins, CO 80523 2025, USA
Trans R Soc Trop Med Hyg 102:S145-51. 2008.... - mini-Tn7 insertion in bacteria with multiple glmS-linked attTn7 sites: example Burkholderia mallei ATCC 23344Kyoung-Hee Choi
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523-1682, USA
Nat Protoc 1:162-9. 2006..This first chromosome integration system in B. mallei provides an important contribution to the genetic tools emerging for Burkholderia spp. Vectors are available for gene complementation and expression, and gene fusion analyses... - High-level triclosan resistance in Pseudomonas aeruginosa is solely a result of effluxRungtip Chuanchuen
Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, USA
Am J Infect Control 31:124-7. 2003..RESULTS: The results showed that the ability of P aeruginosa to survive in the presence of triclosan concentrations in excess of 1,000 microg/mL is solely attributable to the expression of efflux pumps... - mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosaKyoung-Hee Choi
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523-1682, USA
Nat Protoc 1:153-61. 2006..Vectors have been developed for gene complementation, construction of gene fusions, regulated gene expression and reporter gene tagging... - Mini-Tn7 vectors as genetic tools for gene cloning at a single copy number in an industrially important and phytopathogenic bacteria, Xanthomonas sppThichakorn Jittawuttipoka
Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand
FEMS Microbiol Lett 298:111-7. 2009..Mini-Tn7 insertion did not affect bacterial virulence on the tested plant. The mini-Tn7 and FLP-FRT systems also work well in Xanthomonas oryzae pv. oryzae... - Management of accidental laboratory exposure to Burkholderia pseudomallei and B. malleiSharon J Peacock
Mahidol University, Bangkok, Thailand
Emerg Infect Dis 14:e2. 2008..pseudomallei. The overall mortality rate of infected persons is 50% in northeast Thailand (35% in children) and 19% in Australia... - Bacterial resistance to antibiotics: active efflux and reduced uptakeAyush Kumar
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA
Adv Drug Deliv Rev 57:1486-513. 2005..Recent advances in understanding the physical structures, function and regulation of efflux systems will facilitate exploitation of pumps as new drug targets... - Immunotherapy markedly increases the effectiveness of antimicrobial therapy for treatment of Burkholderia pseudomallei infectionKatie L Propst
Department of Microbiology, Immunology, and Pathology, Colorado State University, Ft Collins, CO 80523, USA
Antimicrob Agents Chemother 54:1785-92. 2010..We concluded, therefore, that immunotherapy with either endogenous or exogenous IFN-gamma could significantly increase the effectiveness of conventional antimicrobial therapy for the treatment of acute B. pseudomallei infection... - Pseudomonas aeruginosa-plant root interactions. Pathogenicity, biofilm formation, and root exudationTravis S Walker
Department of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, Colorado 80523, USA
Plant Physiol 134:320-31. 2004..aeruginosa. Our results collectively suggest that upon root colonization, P. aeruginosa forms a biofilm that confers resistance against root-secreted antibiotics... - mini-Tn7 insertion in bacteria with secondary, non-glmS-linked attTn7 sites: example Proteus mirabilis HI4320Kyoung-Hee Choi
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523-1682, USA
Nat Protoc 1:170-8. 2006..Vectors are available for gene complementation and expression, gene fusion analyses and tagging with a green fluorescent protein (GFP)-encoding reporter gene... - Beta-ketoacyl acyl carrier protein reductase (FabG) activity of the fatty acid biosynthetic pathway is a determining factor of 3-oxo-homoserine lactone acyl chain lengthsTung T Hoang
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523-1682A, USA
Microbiology 148:3849-56. 2002.... - A Tn7-based broad-range bacterial cloning and expression systemKyoung-Hee Choi
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
Nat Methods 2:443-8. 2005..pestis. This system will thus have widespread biomedical and environmental applications, especially in environments where plasmids and antibiotic selection are not feasible, namely in plant and animal models or biofilms... - Genetic characterization of pcpS, encoding the multifunctional phosphopantetheinyl transferase of Pseudomonas aeruginosaNazir Barekzi
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523-1658, USA
Microbiology 150:795-803. 2004..The data presented here for the first time confirm that PcpS plays an essential role in both fatty acid and siderophore metabolism... - A Burkholderia pseudomallei deltapurM mutant is avirulent in immunocompetent and immunodeficient animals: candidate strain for exclusion from select-agent listsKatie L Propst
Department of Microbiology, Immunology and Pathology, Rocky Mountain Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research, Colorado State University, Fort Collins, Colorado 80523 2025, USA
Infect Immun 78:3136-43. 2010..We concluded that DeltapurM mutant Bp82 is fully attenuated and safe for use under BSL-2 laboratory conditions and thus is a candidate for exclusion from the select-agent list... - Global gene expression profiles suggest an important role for nutrient acquisition in early pathogenesis in a plant model of Pseudomonas aeruginosa infectionTiffany L Weir
1173 Campus Delivery, Department of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, CO 80523 1173, USA
Appl Environ Microbiol 74:5784-91. 2008..Indeed, there are several reports suggesting that P. aeruginosa virulence is influenced in mammalian hosts by the availability of micronutrients, such as iron and nitrogen, and by levels of O(2)... - Molecular basis of azithromycin-resistant Pseudomonas aeruginosa biofilmsRichard J Gillis
Department of Microbiology and Immunology, Box 672, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
Antimicrob Agents Chemother 49:3858-67. 2005..mexA, which is constitutively expressed in planktonic cells, was uniformly expressed in biofilms regardless of the presence of AZM. These data indicate that the MexCD-OprJ pump acts as a biofilm-specific mechanism for AZM resistance... - Structure of the Pseudomonas aeruginosa acyl-homoserinelactone synthase LasITy A Gould
Department of Pharmacology, Program in Biomolecular Structure, The University of Colorado Health Sciences Center, 4200 E Ninth Ave, Denver, CO 80262, USA
Mol Microbiol 53:1135-46. 2004..This structure and the novel explanation of AHL-synthase acyl-chain-length selectivity promise to guide the design of Pseudomonas aeruginosa-specific quorum-sensing inhibitors as antibacterial agents... - Quorum-sensing signal synthesis by the Yersinia pestis acyl-homoserine lactone synthase YspIJ Paul Kirwan
Department of Pharmacology, Program in Biomolecular Structure, The University of Colorado Health Sciences Center, P.O. Box 8511 MS8303, Aurora, CO 80045, USA
J Bacteriol 188:784-8. 2006..Analysis by liquid chromatography-mass spectrometry revealed that the predominant AHLs were N-3-oxooctanoyl-L-homoserine lactone and N-3-oxo-hexanoyl-L-homoserine lactone... - Proteome analysis of extracellular proteins regulated by the las and rhl quorum sensing systems in Pseudomonas aeruginosa PAO1Amanda S Nouwens
Australian Proteome Analysis Facility, Level 4, Building F7B, Macquarie University, Australia 2109
Microbiology 149:1311-22. 2003..Known QS-regulated extracellular proteins, including elastase (lasB), LasA protease (lasA) and alkaline metalloproteinase (aprA) were also detected... - Two-pronged survival strategy for the major cystic fibrosis pathogen, Pseudomonas aeruginosa, lacking the capacity to degrade nitric oxide during anaerobic respirationSang Sun Yoon
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH 45267 0524, USA
EMBO J 26:3662-72. 2007..These data suggest that protection of a P. aeruginosa norCB mutant against anaerobic NO toxicity occurs by both control of NO supply and reassignment of metabolic enzymes to the task of NO sequestration... - Crystallization of Pseudomonas aeruginosa AHL synthase LasI using beta-turn crystal engineeringTy A Gould
Department of Pharmacology, The University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262, USA
Acta Crystallogr D Biol Crystallogr 60:518-20. 2004..The resulting protein is active, more stable than the wild-type LasI and has been crystallized in the cubic space group F23, with unit-cell parameters a = b = c = 154.90 A... - A high-throughput, homogeneous, bioluminescent assay for Pseudomonas aeruginosa gyrase inhibitors and other DNA-damaging agentsDonald T Moir
Microbiotix, Inc, Worcester, Massachusetts 01605, USA
J Biomol Screen 12:855-64. 2007..This convenient, inexpensive screen will be useful for rapidly identifying DNA gyrase inhibitors and other DNA-damaging agents, which may lead to potent new antibacterials... - Two aerobic pathways for the formation of unsaturated fatty acids in Pseudomonas aeruginosaKun Zhu
Department of Infectious Diseases, Protein Science Division, St Jude Children's Research Hospital, Memphis, TN 38105, USA
Mol Microbiol 60:260-73. 2006....
Research Grants
- Genetic Tools for Pathogenic BacteriaHerbert Schweizer; Fiscal Year: 2005....
- Non-antiobiotic resistance markers for bacteriaHerbert Schweizer; Fiscal Year: 2006..mallei, B. pseudomallei, F. tularensis and Y. pestis, as well as other bacterial pathogens for which one faces similar restrictions regarding use of antibiotic selection markers for genetic manipulation. ..
- Regulation and function of Pseudomonas drug efflux pumpsHerbert Schweizer; Fiscal Year: 2006..Aim 3: Probe other, perhaps global regulators of efflux operon expression, specifically MexS and PA4878. ..
- PSEUDOMONAS AUTOINDUCER BIOSYNTHESIS AND DRUG DISCOVERYHerbert Schweizer; Fiscal Year: 2001..These studies should provide a better understanding of the quorum sensing pathways and lead to the identification of novel anti microbial targets and antimicrobials effective against gram-negative pathogens. ..