Herbert Schweizer

Summary

Affiliation: Colorado State University
Country: USA

Publications

  1. pmc Antimicrobial drug-selection markers for Burkholderia pseudomallei and B. mallei
    Herbert P Schweizer
    Rocky Mountain Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Emerg Infect Dis 14:1689-92. 2008
  2. pmc φX216, a P2-like bacteriophage with broad Burkholderia pseudomallei and B. mallei strain infectivity
    Brian H Kvitko
    Department of Microbiology, Colorado State University, IDRC at Foothills Campus, Fort Collins, CO 80523 0922, USA
    BMC Microbiol 12:289. 2012
  3. pmc Mechanisms of antibiotic resistance in Burkholderia pseudomallei: implications for treatment of melioidosis
    Herbert P Schweizer
    Colorado State University, Department of Microbiology, Immunology and Pathology, IDRC at Foothills Campus, 0922 Campus Delivery, Fort Collins, CO 80523, USA
    Future Microbiol 7:1389-99. 2012
  4. doi request reprint Understanding efflux in Gram-negative bacteria: opportunities for drug discovery
    Herbert P Schweizer
    Colorado State University, IDRC at Foothills Campus, Department of Microbiology, Immunology and Pathology, Fort Collins, CO 80523 0922, USA
    Expert Opin Drug Discov 7:633-42. 2012
  5. pmc A simple method for construction of pir+ Enterobacterial hosts for maintenance of R6K replicon plasmids
    Brian H Kvitko
    Department of Microbiology, Immunology and Pathology, Colorado State University, IDRC at Foothills Campus, 0922 Campus Delivery, Fort Collins, CO 80523, USA
    BMC Res Notes 5:157. 2012
  6. pmc An improved method for rapid generation of unmarked Pseudomonas aeruginosa deletion mutants
    Kyoung Hee Choi
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA
    BMC Microbiol 5:30. 2005
  7. doi request reprint Bacterial genetics: past achievements, present state of the field, and future challenges
    Herbert Schweizer
    Rocky Mountain Regional Center for Biodefense and Emerging Infectious Diseases Research, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1690, USA
    Biotechniques 44:633-4, 636-41. 2008
  8. pmc Methods for genetic manipulation of Burkholderia gladioli pathovar cocovenenans
    Nawarat Somprasong
    Department of Biotechnology, Faculty of Science, Mahidol University, Ratchathewi, Bangkok, Thailand, 10400
    BMC Res Notes 3:308. 2010
  9. ncbi request reprint Triclosan: a widely used biocide and its link to antibiotics
    H P Schweizer
    Department of Microbiology, Colorado State University, Fort Collins, CO 80523 1677, USA
    FEMS Microbiol Lett 202:1-7. 2001
  10. ncbi request reprint Efflux as a mechanism of resistance to antimicrobials in Pseudomonas aeruginosa and related bacteria: unanswered questions
    Herbert P Schweizer
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682A, USA
    Genet Mol Res 2:48-62. 2003

Research Grants

  1. Genetic Tools for Pathogenic Bacteria
    Herbert Schweizer; Fiscal Year: 2005
  2. Non-antiobiotic resistance markers for bacteria
    Herbert Schweizer; Fiscal Year: 2006
  3. Regulation and function of Pseudomonas drug efflux pumps
    Herbert Schweizer; Fiscal Year: 2006
  4. PSEUDOMONAS AUTOINDUCER BIOSYNTHESIS AND DRUG DISCOVERY
    Herbert Schweizer; Fiscal Year: 2001

Collaborators

  • Rungtip Chuanchuen
  • William C Nierman
  • Bart J Currie
  • Kyoung Hee Choi
  • Sharon J Peacock
  • Skorn Mongkolsuk
  • Allen C Cheng
  • Mayuree Fuangthong
  • Paiboon Vattanaviboon
  • Naomasa Gotoh
  • Jay E Gee
  • I Steinmetz
  • D A Dance
  • Donald T Moir
  • Yong Mei Zhang
  • Patricia J Kiley
  • C O Rock
  • Ayush Kumar
  • Takehiko Mima
  • Brian H Kvitko
  • Katie L Propst
  • Lily A Trunck
  • Ty A Gould
  • Drew A Rholl
  • Andrew Goodyear
  • Mair E A Churchill
  • Ian McMillan
  • Nawarat Somprasong
  • Steven W Dow
  • Carolina M Lopez
  • Thichakorn Jittawuttipoka
  • Tiffany L Weir
  • Sang Sun Yoon
  • J Paul Kirwan
  • Kun Zhu
  • Swati Joshi
  • Richard J Gillis
  • Travis S Walker
  • Nazir Barekzi
  • Amanda S Nouwens
  • Tung T Hoang
  • Stephanie Lehman
  • David DeShazer
  • John Prucha
  • Kent J Voorhees
  • Helle Bielefeldt-Ohmann
  • RoxAnn Karkhoff-Schweizer
  • Shannon L Johnson
  • Steven Bruckbauer
  • Steven Dow
  • Katie Mladinich
  • Christopher R Cox
  • Erin J Breland
  • Ryan M Troyer
  • Lisa M Kellihan
  • RoxAnn R Karkhoff-Schweizer
  • Sarinya Buranajitpakorn
  • Valerie J Stull
  • Mark Mayo
  • Dayakar Badri
  • Jorge Vivanco
  • Robert F Hennigan
  • Stephanie L Groce
  • Margarita Gomez-Escalada
  • Michael L Howell
  • Ahmet C Karabulut
  • Benjamin Gaston
  • Daniel J Hassett
  • Sergei V Lymar
  • John D Lipscomb
  • Andrew B Herr
  • Michael J Schurr
  • Scott W Bearden
  • Robert C Murphy
  • Kim Lee Chua
  • Victoria E Wagner
  • Barbara H Iglewski
  • Kimberly G White
  • Todd Ontl
  • Scott Irwin
  • Ray Fall
  • Harsh Pal Bais
  • Laurence G Rahme
  • Jorge M Vivanco
  • William T Watson
  • Eric Déziel
  • Stuart J Cordwell
  • Scott A Beatson
  • Cynthia B Whitchurch
  • Bradley J Walsh

Detail Information

Publications49

  1. pmc Antimicrobial drug-selection markers for Burkholderia pseudomallei and B. mallei
    Herbert P Schweizer
    Rocky Mountain Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Emerg Infect Dis 14:1689-92. 2008
    ..Deliberation and decisions regarding alternative selection markers (antimicrobial and nonantimicrobial drugs) by the international community, regulatory authorities, and funding agencies are needed...
  2. pmc φX216, a P2-like bacteriophage with broad Burkholderia pseudomallei and B. mallei strain infectivity
    Brian H Kvitko
    Department of Microbiology, Colorado State University, IDRC at Foothills Campus, Fort Collins, CO 80523 0922, USA
    BMC Microbiol 12:289. 2012
    ..pseudomallei genome content due in part to the presence or absence of integrated prophages. Several phages have previously been isolated from B. pseudomallei lysogens, for example φK96243, φ1026b and φ52237...
  3. pmc Mechanisms of antibiotic resistance in Burkholderia pseudomallei: implications for treatment of melioidosis
    Herbert P Schweizer
    Colorado State University, Department of Microbiology, Immunology and Pathology, IDRC at Foothills Campus, 0922 Campus Delivery, Fort Collins, CO 80523, USA
    Future Microbiol 7:1389-99. 2012
    ..Novel agents and therapeutic strategies are being tested and, in some instances, show promise as anti-B. pseudomallei infectives...
  4. doi request reprint Understanding efflux in Gram-negative bacteria: opportunities for drug discovery
    Herbert P Schweizer
    Colorado State University, IDRC at Foothills Campus, Department of Microbiology, Immunology and Pathology, Fort Collins, CO 80523 0922, USA
    Expert Opin Drug Discov 7:633-42. 2012
    ..However, recent advances in our understanding of efflux in these bacteria provide opportunities and assets for drug discovery...
  5. pmc A simple method for construction of pir+ Enterobacterial hosts for maintenance of R6K replicon plasmids
    Brian H Kvitko
    Department of Microbiology, Immunology and Pathology, Colorado State University, IDRC at Foothills Campus, 0922 Campus Delivery, Fort Collins, CO 80523, USA
    BMC Res Notes 5:157. 2012
    ..In Escherichia coli this is commonly achieved by chromosomal integration of pir either via lysogenization with a λpir phage or homologous recombination at a pre-determined locus...
  6. pmc An improved method for rapid generation of unmarked Pseudomonas aeruginosa deletion mutants
    Kyoung Hee Choi
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA
    BMC Microbiol 5:30. 2005
    ..PCR and recombinational technologies can be exploited to substantially accelerate virtually all steps involved in the gene replacement process...
  7. doi request reprint Bacterial genetics: past achievements, present state of the field, and future challenges
    Herbert Schweizer
    Rocky Mountain Regional Center for Biodefense and Emerging Infectious Diseases Research, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1690, USA
    Biotechniques 44:633-4, 636-41. 2008
    ..BioTechniques has played an integral part in the publication of important technological advances in the field over the first 25 years of its existence and it can be anticipated that it will continue to do so in the future...
  8. pmc Methods for genetic manipulation of Burkholderia gladioli pathovar cocovenenans
    Nawarat Somprasong
    Department of Biotechnology, Faculty of Science, Mahidol University, Ratchathewi, Bangkok, Thailand, 10400
    BMC Res Notes 3:308. 2010
    ..Little is known about the regulation of virulence factor and toxin production in BGC, and studies in this bacterium have been hampered by lack of genetic tools...
  9. ncbi request reprint Triclosan: a widely used biocide and its link to antibiotics
    H P Schweizer
    Department of Microbiology, Colorado State University, Fort Collins, CO 80523 1677, USA
    FEMS Microbiol Lett 202:1-7. 2001
    ....
  10. ncbi request reprint Efflux as a mechanism of resistance to antimicrobials in Pseudomonas aeruginosa and related bacteria: unanswered questions
    Herbert P Schweizer
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682A, USA
    Genet Mol Res 2:48-62. 2003
    ..Despite intensive studies of these multidrug efflux systems over the past several years, their precise molecular architectures, their modes of regulation of expression and their natural functions remain largely unknown...
  11. ncbi request reprint Applications of the Saccharomyces cerevisiae Flp-FRT system in bacterial genetics
    Herbert P Schweizer
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, 80523 1682, USA
    J Mol Microbiol Biotechnol 5:67-77. 2003
    ..On the contrary, in many instances this technology is probably greatly underutilized, especially in gram-positive bacteria...
  12. ncbi request reprint Vectors to express foreign genes and techniques to monitor gene expression in Pseudomonads
    H P Schweizer
    Department of Microbiology, Colorado State University, Fort Collins, CO 80523, USA
    Curr Opin Biotechnol 12:439-45. 2001
    ....
  13. pmc The MexJK efflux pump of Pseudomonas aeruginosa requires OprM for antibiotic efflux but not for efflux of triclosan
    Rungtip Chuanchuen
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523 1682, USA
    J Bacteriol 184:5036-44. 2002
    ..Furthermore, the results confirm that triclosan is an excellent tool for the study of RND multidrug efflux systems and that this popular biocide therefore readily selects mutants which are cross-resistant with antibiotics...
  14. pmc Method for regulated expression of single-copy efflux pump genes in a surrogate Pseudomonas aeruginosa strain: identification of the BpeEF-OprC chloramphenicol and trimethoprim efflux pump of Burkholderia pseudomallei 1026b
    Ayush Kumar
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
    Antimicrob Agents Chemother 50:3460-3. 2006
    ..Using this method, the Burkholderia pseudomallei bpeEF-oprC operon was shown to encode a chloramphenicol and trimethoprim efflux pump...
  15. pmc Substrate-dependent utilization of OprM or OpmH by the Pseudomonas aeruginosa MexJK efflux pump
    Rungtip Chuanchuen
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Antimicrob Agents Chemother 49:2133-6. 2005
    ..This is the first report of natural utilization of multiple OMPs by a given resistance nodulation cell division transporter/membrane fusion protein pair...
  16. ncbi request reprint Benchtop and microcentrifuge preparation of Pseudomonas aeruginosa competent cells
    Rungtip Chuanchuen
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins 80523 1682A, USA
    Biotechniques 33:760, 762-3. 2002
  17. doi request reprint Role of the MexXY multidrug efflux pump in moderate aminoglycoside resistance in Pseudomonas aeruginosa isolates from Pseudomonas mastitis
    Rungtip Chuanchuen
    Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand
    Microbiol Immunol 52:392-8. 2008
    ..Transcription levels of mexY were independent on mutations in mexZ, suggesting the existence of additional regulatory mechanisms other than mexZ...
  18. pmc The BpeAB-OprB efflux pump of Burkholderia pseudomallei 1026b does not play a role in quorum sensing, virulence factor production, or extrusion of aminoglycosides but is a broad-spectrum drug efflux system
    Takehiko Mima
    Department of Microbiology, Immunology and Pathology, Colorado State University, IDRC at Foothills Campus, Campus Delivery 2025, Fort Collins, CO 80523 2025, USA
    Antimicrob Agents Chemother 54:3113-20. 2010
    ..At present, we do not know why our BpeAB-OprB susceptibility and virulence factor expression results with 1026b and its derivatives are different from those previously published for Singapore strain KHW...
  19. ncbi request reprint Functional characterization of MexXY and OpmG in aminoglycoside efflux in Pseudomonas aeruginosa
    Rungtip Chuanchuen
    Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand
    Southeast Asian J Trop Med Public Health 39:115-22. 2008
    ..Growth rates of wildtype P. aeruginosa and opmG mutant derivatives were not different, indicating that expression of opmG is not essential for P. aeruginosa growth...
  20. pmc Identification and characterization of TriABC-OpmH, a triclosan efflux pump of Pseudomonas aeruginosa requiring two membrane fusion proteins
    Takehiko Mima
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
    J Bacteriol 189:7600-9. 2007
    ..TriABC is the fifth RND pump in P. aeruginosa shown to efficiently efflux triclosan, supporting the notion that efflux is the primary mechanism responsible for this bacterium's high intrinsic and acquired triclosan resistance...
  21. pmc Genetic tools for select-agent-compliant manipulation of Burkholderia pseudomallei
    Kyoung Hee Choi
    Department of Microbiology, Immunology and Pathology, Rocky Mountain Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research, Colorado State University, Fort Collins, CO 80523 1682, USA
    Appl Environ Microbiol 74:1064-75. 2008
    ..The new tools allow routine select-agent-compliant genetic manipulations of B. pseudomallei and other Burkholderia species...
  22. pmc In vivo Himar1 transposon mutagenesis of Burkholderia pseudomallei
    Drew A Rholl
    Department of Microbiology, Immunology and Pathology, Rocky Mountain Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research, Colorado State University, Fort Collins, CO 80523 1690, USA
    Appl Environ Microbiol 74:7529-35. 2008
    ..mallei. Our results demonstrate that comprehensive transposon libraries of B. pseudomallei can be generated, providing additional tools for the study of the biology, pathogenesis, and antibiotic resistance of this pathogen...
  23. pmc Versatile dual-technology system for markerless allele replacement in Burkholderia pseudomallei
    Carolina M Lopez
    Department of Microbiology, Immunology and Pathology, Colorado State University, IDRC at Foothills Campus, Campus Delivery 2025, Fort Collins, CO 80523 2025, USA
    Appl Environ Microbiol 75:6496-503. 2009
    ..pseudomallei strains. Finally, sacB-based counterselection was employed to isolate a defined chromosomal fabD(Ts) allele that causes synthesis of a temperature-sensitive FabD, an essential fatty acid biosynthesis enzyme...
  24. ncbi request reprint A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformation
    Kyoung Hee Choi
    Department of Microbiology, Immunology and Pathology, Colorado State University, 1682 Campus Delivery, Fort Collins, CO 80523, USA
    J Microbiol Methods 64:391-7. 2006
    ....
  25. pmc Molecular characterization of MexL, the transcriptional repressor of the mexJK multidrug efflux operon in Pseudomonas aeruginosa
    Rungtip Chuanchuen
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
    Antimicrob Agents Chemother 49:1844-51. 2005
    ..The MexL-protected region contains two inverted GTATTT repeats, and their location in the protected region and overlap with the mexL and mexJ promoter sequences strongly support a role in MexL binding...
  26. ncbi request reprint High-level triclosan resistance in Pseudomonas aeruginosa is solely a result of efflux
    Rungtip Chuanchuen
    Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, USA
    Am J Infect Control 31:124-7. 2003
    ..Pseudomonas aeruginosa is intrinsically resistant to high levels of triclosan...
  27. doi request reprint Expression of resistance-nodulation-cell-division efflux pumps in commonly used Burkholderia pseudomallei strains and clinical isolates from northern Australia
    Ayush Kumar
    Department of Microbiology, Immunology, and Pathology, Rocky Mountain Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research, Colorado State University, Fort Collins, CO 80523 2025, USA
    Trans R Soc Trop Med Hyg 102:S145-51. 2008
    ....
  28. ncbi request reprint mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa
    Kyoung Hee Choi
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523 1682, USA
    Nat Protoc 1:153-61. 2006
    ..Vectors have been developed for gene complementation, construction of gene fusions, regulated gene expression and reporter gene tagging...
  29. ncbi request reprint mini-Tn7 insertion in bacteria with multiple glmS-linked attTn7 sites: example Burkholderia mallei ATCC 23344
    Kyoung Hee Choi
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523 1682, USA
    Nat Protoc 1:162-9. 2006
    ..This first chromosome integration system in B. mallei provides an important contribution to the genetic tools emerging for Burkholderia spp. Vectors are available for gene complementation and expression, and gene fusion analyses...
  30. pmc Persistent gastric colonization with Burkholderia pseudomallei and dissemination from the gastrointestinal tract following mucosal inoculation of mice
    Andrew Goodyear
    Department of Microbiology, Immunology, and Pathology, Rocky Mountain Regional Center for Excellence for Biodefense and Emerging Infectious Diseases Research, Colorado State University, Fort Collins, Colorado, United States of America
    PLoS ONE 7:e37324. 2012
    ..pseudomallei preferentially colonizes the stomach following oral inoculation, and that the chronically colonized GI tract likely serves as a reservoir for dissemination of infection to extra-intestinal sites...
  31. pmc Immunotherapy markedly increases the effectiveness of antimicrobial therapy for treatment of Burkholderia pseudomallei infection
    Katie L Propst
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Ft Collins, CO 80523, USA
    Antimicrob Agents Chemother 54:1785-92. 2010
    ..We concluded, therefore, that immunotherapy with either endogenous or exogenous IFN-gamma could significantly increase the effectiveness of conventional antimicrobial therapy for the treatment of acute B. pseudomallei infection...
  32. pmc Mini-Tn7 vectors as genetic tools for gene cloning at a single copy number in an industrially important and phytopathogenic bacteria, Xanthomonas spp
    Thichakorn Jittawuttipoka
    Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand
    FEMS Microbiol Lett 298:111-7. 2009
    ..Mini-Tn7 insertion did not affect bacterial virulence on the tested plant. The mini-Tn7 and FLP-FRT systems also work well in Xanthomonas oryzae pv. oryzae...
  33. pmc Pseudomonas aeruginosa-plant root interactions. Pathogenicity, biofilm formation, and root exudation
    Travis S Walker
    Department of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, Colorado 80523, USA
    Plant Physiol 134:320-31. 2004
    ..aeruginosa. Our results collectively suggest that upon root colonization, P. aeruginosa forms a biofilm that confers resistance against root-secreted antibiotics...
  34. ncbi request reprint Beta-ketoacyl acyl carrier protein reductase (FabG) activity of the fatty acid biosynthetic pathway is a determining factor of 3-oxo-homoserine lactone acyl chain lengths
    Tung T Hoang
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682A, USA
    Microbiology 148:3849-56. 2002
    ....
  35. ncbi request reprint A Tn7-based broad-range bacterial cloning and expression system
    Kyoung Hee Choi
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Nat Methods 2:443-8. 2005
    ..pestis. This system will thus have widespread biomedical and environmental applications, especially in environments where plasmids and antibiotic selection are not feasible, namely in plant and animal models or biofilms...
  36. ncbi request reprint Bacterial resistance to antibiotics: active efflux and reduced uptake
    Ayush Kumar
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA
    Adv Drug Deliv Rev 57:1486-513. 2005
    ..Recent advances in understanding the physical structures, function and regulation of efflux systems will facilitate exploitation of pumps as new drug targets...
  37. pmc Management of accidental laboratory exposure to Burkholderia pseudomallei and B. mallei
    Sharon J Peacock
    Mahidol University, Bangkok, Thailand
    Emerg Infect Dis 14:e2. 2008
    ..pseudomallei. The overall mortality rate of infected persons is 50% in northeast Thailand (35% in children) and 19% in Australia...
  38. ncbi request reprint mini-Tn7 insertion in bacteria with secondary, non-glmS-linked attTn7 sites: example Proteus mirabilis HI4320
    Kyoung Hee Choi
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523 1682, USA
    Nat Protoc 1:170-8. 2006
    ..Vectors are available for gene complementation and expression, gene fusion analyses and tagging with a green fluorescent protein (GFP)-encoding reporter gene...
  39. pmc Global gene expression profiles suggest an important role for nutrient acquisition in early pathogenesis in a plant model of Pseudomonas aeruginosa infection
    Tiffany L Weir
    1173 Campus Delivery, Department of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, CO 80523 1173, USA
    Appl Environ Microbiol 74:5784-91. 2008
    ..Indeed, there are several reports suggesting that P. aeruginosa virulence is influenced in mammalian hosts by the availability of micronutrients, such as iron and nitrogen, and by levels of O(2)...
  40. pmc A Burkholderia pseudomallei deltapurM mutant is avirulent in immunocompetent and immunodeficient animals: candidate strain for exclusion from select-agent lists
    Katie L Propst
    Department of Microbiology, Immunology and Pathology, Rocky Mountain Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research, Colorado State University, Fort Collins, Colorado 80523 2025, USA
    Infect Immun 78:3136-43. 2010
    ..We concluded that DeltapurM mutant Bp82 is fully attenuated and safe for use under BSL-2 laboratory conditions and thus is a candidate for exclusion from the select-agent list...
  41. ncbi request reprint Genetic characterization of pcpS, encoding the multifunctional phosphopantetheinyl transferase of Pseudomonas aeruginosa
    Nazir Barekzi
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1658, USA
    Microbiology 150:795-803. 2004
    ..The data presented here for the first time confirm that PcpS plays an essential role in both fatty acid and siderophore metabolism...
  42. pmc Molecular basis of azithromycin-resistant Pseudomonas aeruginosa biofilms
    Richard J Gillis
    Department of Microbiology and Immunology, Box 672, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Antimicrob Agents Chemother 49:3858-67. 2005
    ..mexA, which is constitutively expressed in planktonic cells, was uniformly expressed in biofilms regardless of the presence of AZM. These data indicate that the MexCD-OprJ pump acts as a biofilm-specific mechanism for AZM resistance...
  43. ncbi request reprint Crystallization of Pseudomonas aeruginosa AHL synthase LasI using beta-turn crystal engineering
    Ty A Gould
    Department of Pharmacology, The University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262, USA
    Acta Crystallogr D Biol Crystallogr 60:518-20. 2004
    ..The resulting protein is active, more stable than the wild-type LasI and has been crystallized in the cubic space group F23, with unit-cell parameters a = b = c = 154.90 A...
  44. ncbi request reprint Proteome analysis of extracellular proteins regulated by the las and rhl quorum sensing systems in Pseudomonas aeruginosa PAO1
    Amanda S Nouwens
    Australian Proteome Analysis Facility, Level 4, Building F7B, Macquarie University, Australia 2109
    Microbiology 149:1311-22. 2003
    ..Known QS-regulated extracellular proteins, including elastase (lasB), LasA protease (lasA) and alkaline metalloproteinase (aprA) were also detected...
  45. ncbi request reprint Structure of the Pseudomonas aeruginosa acyl-homoserinelactone synthase LasI
    Ty A Gould
    Department of Pharmacology, Program in Biomolecular Structure, The University of Colorado Health Sciences Center, 4200 E Ninth Ave, Denver, CO 80262, USA
    Mol Microbiol 53:1135-46. 2004
    ..This structure and the novel explanation of AHL-synthase acyl-chain-length selectivity promise to guide the design of Pseudomonas aeruginosa-specific quorum-sensing inhibitors as antibacterial agents...
  46. pmc Two-pronged survival strategy for the major cystic fibrosis pathogen, Pseudomonas aeruginosa, lacking the capacity to degrade nitric oxide during anaerobic respiration
    Sang Sun Yoon
    Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH 45267 0524, USA
    EMBO J 26:3662-72. 2007
    ..These data suggest that protection of a P. aeruginosa norCB mutant against anaerobic NO toxicity occurs by both control of NO supply and reassignment of metabolic enzymes to the task of NO sequestration...
  47. pmc A high-throughput, homogeneous, bioluminescent assay for Pseudomonas aeruginosa gyrase inhibitors and other DNA-damaging agents
    Donald T Moir
    Microbiotix, Inc, Worcester, Massachusetts 01605, USA
    J Biomol Screen 12:855-64. 2007
    ..This convenient, inexpensive screen will be useful for rapidly identifying DNA gyrase inhibitors and other DNA-damaging agents, which may lead to potent new antibacterials...
  48. ncbi request reprint Two aerobic pathways for the formation of unsaturated fatty acids in Pseudomonas aeruginosa
    Kun Zhu
    Department of Infectious Diseases, Protein Science Division, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Mol Microbiol 60:260-73. 2006
    ....
  49. pmc Quorum-sensing signal synthesis by the Yersinia pestis acyl-homoserine lactone synthase YspI
    J Paul Kirwan
    Department of Pharmacology, Program in Biomolecular Structure, The University of Colorado Health Sciences Center, P O Box 8511 MS8303, Aurora, CO 80045, USA
    J Bacteriol 188:784-8. 2006
    ..Analysis by liquid chromatography-mass spectrometry revealed that the predominant AHLs were N-3-oxooctanoyl-L-homoserine lactone and N-3-oxo-hexanoyl-L-homoserine lactone...

Research Grants11

  1. Genetic Tools for Pathogenic Bacteria
    Herbert Schweizer; Fiscal Year: 2005
    ....
  2. Non-antiobiotic resistance markers for bacteria
    Herbert Schweizer; Fiscal Year: 2006
    ..mallei, B. pseudomallei, F. tularensis and Y. pestis, as well as other bacterial pathogens for which one faces similar restrictions regarding use of antibiotic selection markers for genetic manipulation. ..
  3. Regulation and function of Pseudomonas drug efflux pumps
    Herbert Schweizer; Fiscal Year: 2006
    ..Aim 3: Probe other, perhaps global regulators of efflux operon expression, specifically MexS and PA4878. ..
  4. PSEUDOMONAS AUTOINDUCER BIOSYNTHESIS AND DRUG DISCOVERY
    Herbert Schweizer; Fiscal Year: 2001
    ..These studies should provide a better understanding of the quorum sensing pathways and lead to the identification of novel anti microbial targets and antimicrobials effective against gram-negative pathogens. ..