Ian Orme

Summary

Affiliation: Colorado State University
Country: USA

Publications

  1. pmc A new unifying theory of the pathogenesis of tuberculosis
    Ian M Orme
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA Electronic address
    Tuberculosis (Edinb) 94:8-14. 2014
  2. pmc Vaccine development for tuberculosis: current progress
    Ian M Orme
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Drugs 73:1015-24. 2013
  3. doi request reprint The Achilles heel of BCG
    Ian M Orme
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Tuberculosis (Edinb) 90:329-32. 2010
  4. ncbi request reprint Current progress in tuberculosis vaccine development
    Ian M Orme
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Vaccine 23:2105-8. 2005
  5. ncbi request reprint Tuberculosis vaccines: current progress
    Ian M Orme
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Drugs 65:2437-44. 2005
  6. ncbi request reprint The use of animal models to guide rational vaccine design
    Ian M Orme
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Microbes Infect 7:905-10. 2005
  7. ncbi request reprint Preclinical testing of new vaccines for tuberculosis: a comprehensive review
    Ian M Orme
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Lake Street, Fort Collins, CO 80523, USA
    Vaccine 24:2-19. 2006
  8. ncbi request reprint Potential complications to TB vaccine testing in animal models
    Ian M Orme
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Indian J Exp Biol 47:440-4. 2009
  9. pmc Development of new vaccines and drugs for TB: limitations and potential strategic errors
    Ian M Orme
    Department of Microbiology, Immunology and Pathology, Colorado State University, Colorado, CO 80523, USA
    Future Microbiol 6:161-77. 2011
  10. ncbi request reprint Safety issues regarding new vaccines for tuberculosis, with an emphasis on post-exposure vaccination
    Ian M Orme
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Tuberculosis (Edinb) 86:68-73. 2006

Research Grants

Collaborators

Detail Information

Publications89

  1. pmc A new unifying theory of the pathogenesis of tuberculosis
    Ian M Orme
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA Electronic address
    Tuberculosis (Edinb) 94:8-14. 2014
    ..I present here a new model of the pathogenesis of the disease that attempts to unify the pathogenic process of infection, disease, persistence [rather than latency], and reactivation. ..
  2. pmc Vaccine development for tuberculosis: current progress
    Ian M Orme
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Drugs 73:1015-24. 2013
    ....
  3. doi request reprint The Achilles heel of BCG
    Ian M Orme
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Tuberculosis (Edinb) 90:329-32. 2010
    ..With no central memory response to compensate, the individual loses any further resistance to tuberculosis. This may have serious implications for vaccine design, given the emphasis on developing recombinant forms of BCG...
  4. ncbi request reprint Current progress in tuberculosis vaccine development
    Ian M Orme
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Vaccine 23:2105-8. 2005
    ....
  5. ncbi request reprint Tuberculosis vaccines: current progress
    Ian M Orme
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Drugs 65:2437-44. 2005
    ..A few of these have already entered early stage clinical trials...
  6. ncbi request reprint The use of animal models to guide rational vaccine design
    Ian M Orme
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Microbes Infect 7:905-10. 2005
    ..There is still much to be learned, however, in terms of rational vaccine design, especially in the context of therapeutic or anti-latent vaccine formulations and animal models of these situations...
  7. ncbi request reprint Preclinical testing of new vaccines for tuberculosis: a comprehensive review
    Ian M Orme
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Lake Street, Fort Collins, CO 80523, USA
    Vaccine 24:2-19. 2006
    ..In addition, no standardized models of safety/toxicology exist as yet, which will be needed before extensive clinical development of the new vaccines...
  8. ncbi request reprint Potential complications to TB vaccine testing in animal models
    Ian M Orme
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Indian J Exp Biol 47:440-4. 2009
    ....
  9. pmc Development of new vaccines and drugs for TB: limitations and potential strategic errors
    Ian M Orme
    Department of Microbiology, Immunology and Pathology, Colorado State University, Colorado, CO 80523, USA
    Future Microbiol 6:161-77. 2011
    ..This includes both the choice of new vaccine and drug candidates, and also the ways these are being tested in animal models, which in the opinion of the author run the risk of driving the field backwards rather than forward...
  10. ncbi request reprint Safety issues regarding new vaccines for tuberculosis, with an emphasis on post-exposure vaccination
    Ian M Orme
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Tuberculosis (Edinb) 86:68-73. 2006
    ..A further element that badly needs more research is the state of immunity already preexisting in the infected host and how vaccine induced immunity may interact with this...
  11. pmc Mycobacteria lacking the RD1 region do not induce necrosis in the lungs of mice lacking interferon-gamma
    Ana Paula Junqueira-Kipnis
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Immunology 119:224-31. 2006
    ..These data support the hypothesis that proteins encoded in the RD1 region are a major cause of necrosis and contribute significantly to the pathogenesis of the disease...
  12. ncbi request reprint XCL1 (lymphotactin) chemokine produced by activated CD8 T cells during the chronic stage of infection with Mycobacterium tuberculosis negatively affects production of IFN-gamma by CD4 T cells and participates in granuloma stability
    Diane Ordway
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
    J Leukoc Biol 82:1221-9. 2007
    ..Additionally, we also describe for the first time that during Mtb infection, activated CD8 T cells in the lungs produce XCL1 and that this chemokine is capable of controlling IFN-gamma production by CD4 T cells...
  13. pmc Post-exposure vaccination against Mycobacterium tuberculosis
    Marcela Henao-Tamayo
    Colorado State University, Fort Collins, CO 80523 1682, USA
    Tuberculosis (Edinb) 89:142-8. 2009
    ....
  14. ncbi request reprint Relative levels of M-CSF and GM-CSF influence the specific generation of macrophage populations during infection with Mycobacterium tuberculosis
    David M Higgins
    Department of Microbiology, Immunology and Pathology, Mycobacteria Research Laboratories, Colorado State University, Fort Collins, CO 80523, USA
    J Immunol 180:4892-900. 2008
    ..In addition, these studies demonstrate that M-CSF may have a role in the adaptive immune response to infection with M. tuberculosis...
  15. ncbi request reprint CD4 is required for the development of a protective granulomatous response to pulmonary tuberculosis
    Bernadette M Saunders
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Cell Immunol 216:65-72. 2002
    ..Early control of M. tuberculosis growth is therefore independent of CD4+ cells but such cells are required to ensure recruitment of mononuclear cells to the lung and thus ensure long-term survival...
  16. pmc Pulmonary necrosis resulting from DNA vaccination against tuberculosis
    Jennifer L Taylor
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Infect Immun 71:2192-8. 2003
    ..tuberculosis. This previously unanticipated safety problem indicates that DNA vaccines should be used with caution in individuals who may have already been exposed to tuberculosis...
  17. pmc Memory T lymphocytes generated by Mycobacterium bovis BCG vaccination reside within a CD4 CD44lo CD62 Ligandhi population
    Andre Kipnis
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Infect Immun 73:7759-64. 2005
    ..Upon cell transfer, however, cells expressing a resting/naïve phenotype (CD44lo CD62Lhi) were capable of protecting the recipients from a virulent challenge infection, suggesting the emergence of T-cell memory from within this subset...
  18. ncbi request reprint Reduced up-regulation of memory and adhesion/integrin molecules in susceptible mice and poor expression of immunity to pulmonary tuberculosis
    Veronica Gruppo
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins 80523, USA
    Microbiology 148:2959-66. 2002
    ..Again, this was associated with poor up-regulation of adhesion and integrin molecules and with histological evidence in memory immune animals of a reduced and delayed influx of T lymphocytes into the lungs...
  19. doi request reprint Lack of IL-10 alters inflammatory and immune responses during pulmonary Mycobacterium tuberculosis infection
    David M Higgins
    Department of Microbiology, Mycobacteria Research Laboratories, Colorado State University, Fort Collins, CO 80523, USA
    Tuberculosis (Edinb) 89:149-57. 2009
    ..tuberculosis infected lung, and the complete removal of this regulatory component eventually leads to disease progression...
  20. pmc The protective effect of the Mycobacterium bovis BCG vaccine is increased by coadministration with the Mycobacterium tuberculosis 72-kilodalton fusion polyprotein Mtb72F in M. tuberculosis-infected guinea pigs
    Lise Brandt
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins 80523, USA
    Infect Immun 72:6622-32. 2004
    ..Collectively, these data suggest that enhancing BCG is a valid vaccination strategy for tuberculosis that is worthy of clinical evaluation...
  21. ncbi request reprint Mice lacking bioactive IL-12 can generate protective, antigen-specific cellular responses to mycobacterial infection only if the IL-12 p40 subunit is present
    Andrea M Cooper
    Mycobacteria Research Laboratories, Department of Microbiology, Colorado State University, Fort Collins, CO 80523, USA
    J Immunol 168:1322-7. 2002
    ..This cytokine is composed of the IL-12 p40 subunit and a p19 subunit. In support of a role for this cytokine in protective responses to M. tuberculosis, we determined that the p19 subunit is induced in the lungs of infected mice...
  22. ncbi request reprint NIH pre-clinical screening program: overview and current status
    Angelo Izzo
    Department of Microbiology, Colorado State University, Immunology and Pathology, Mycobacterial Research Laboratories, 1682 Campus Delivery, Fort Collins 80523 1682, USA
    Tuberculosis (Edinb) 85:25-8. 2005
    ..The current screening program at Colorado State University has tested a wide range of novel vaccine candidates...
  23. pmc Role of chemokine ligand 2 in the protective response to early murine pulmonary tuberculosis
    Andre Kipnis
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Immunology 109:547-51. 2003
    ....
  24. pmc Metronidazole lacks antibacterial activity in guinea pigs infected with Mycobacterium tuberculosis
    Donald R Hoff
    Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523, USA
    Antimicrob Agents Chemother 52:4137-40. 2008
    ..Metronidazole treatment (for 6 weeks at 100 mg/kg of body weight) resulted in no reduction in the bacillary burden and significantly worsened lesion inflammation...
  25. ncbi request reprint Incorporation of CpG oligodeoxynucleotide fails to enhance the protective efficacy of a subunit vaccine against Mycobacterium tuberculosis
    Meng Jer Hsieh
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Microbiology Building, 1682 Campus Delivery, Fort Collins, CO 80523 1682, USA
    Vaccine 22:655-9. 2004
    ..These data suggest that the vaccine enhancing effects of CpG ODN are relatively transient...
  26. ncbi request reprint Prospects for new vaccines against tuberculosis
    Lise Brandt
    Colorado State University, Fort Collins, USA
    Biotechniques 33:1098, 1100, 1102. 2002
  27. pmc A Toll-like receptor-2-directed fusion protein vaccine against tuberculosis
    Baolin Wang
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Clin Vaccine Immunol 14:902-6. 2007
    ....
  28. pmc SWR mice are highly susceptible to pulmonary infection with Mycobacterium tuberculosis
    Oliver C Turner
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Infect Immun 71:5266-72. 2003
    ..We propose that further investigation of the SWR mouse may provide a new animal model for immunocompetent individuals apparently unable to effectively control the growth of M. tuberculosis in the lung...
  29. pmc A limited antigen-specific cellular response is sufficient for the early control of Mycobacterium tuberculosis in the lung but is insufficient for long-term survival
    Joanne Turner
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Infect Immun 72:3759-68. 2004
    ..tuberculosis. This study identifies this lipoprotein as an important and potent inducer of protective T cells within the lungs of mice infected with M. tuberculosis and therefore as a possible target for vaccination...
  30. doi request reprint Preclinical testing of new drugs for tuberculosis: current challenges
    Anne J Lenaerts
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Trends Microbiol 16:48-54. 2008
    ....
  31. pmc Magnetic resonance imaging of pulmonary lesions in guinea pigs infected with Mycobacterium tuberculosis
    Susan L Kraft
    Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, Colorado 80523, USA
    Infect Immun 72:5963-71. 2004
    ..Further development of this technical approach could be very useful in tracking lesion size, number, and progression in the search for new tuberculosis vaccines...
  32. pmc Immune response to Mycobacterium tuberculosis and identification of molecular markers of disease
    Mercedes Gonzalez-Juarrero
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA
    Am J Respir Cell Mol Biol 40:398-409. 2009
    ..tuberculosis infection, and unique molecular markers associated with disease progression and state, were identified...
  33. pmc Intrapulmonary delivery of XCL1-targeting small interfering RNA in mice chronically infected with Mycobacterium tuberculosis
    Adrian G Rosas-Taraco
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
    Am J Respir Cell Mol Biol 41:136-45. 2009
    ....
  34. pmc Immunopathogenesis of pulmonary granulomas in the guinea pig after infection with Mycobacterium tuberculosis
    Oliver C Turner
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins 80523, USA
    Infect Immun 71:864-71. 2003
    ..This hypothesis differs from the current dogma that excessive activity of T cells mediates delayed-type hypersensitivity and that cellular cytolysis is the root cause of the necrosis...
  35. pmc Interleukin-10 production by lung macrophages in CBA xid mutant mice infected with Mycobacterium tuberculosis
    Ana Paula Junqueira-Kipnis
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins 80523, USA
    Immunology 115:246-52. 2005
    ..In addition, macrophages from the lungs of xid mice also expressed high levels of CD14. These observations suggest that the xid mutation in cellular signalling has much wider effects on the immune system than previously thought...
  36. pmc Testing of experimental compounds in a relapse model of tuberculosis using granulocyte-macrophage colony-stimulating factor gene-disrupted mice
    Lisa K Woolhiser
    Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523, USA
    Antimicrob Agents Chemother 53:306-8. 2009
    ..A subsequent treatment with individual drugs was performed to assess their activity on the 1% of remaining bacilli and disease relapse...
  37. doi request reprint Animal models of M. tuberculosis Infection
    Ian Orme
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, USA
    Curr Protoc Microbiol . 2007
    ..Using these protocols the course of infection, the basic immune response, and the extent of lung pathology can be determined in mouse and guinea pig models of experimental tuberculosis...
  38. pmc In vivo adaptation of the Wayne model of latent tuberculosis
    Lisa Woolhiser
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Infect Immun 75:2621-5. 2007
    ..Preimmunization of mice with a panel of selected vaccine candidates slowed or prevented this event. This simple model has potential for identifying vaccines targeting latent tuberculosis...
  39. ncbi request reprint Proteomic approaches to antigen discovery
    Karen M Dobos
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, USA
    Methods Mol Med 94:3-17. 2004
    ..As presented or with minor modifications, these techniques may be universally applied to other bacterial pathogens or used to identify bacterial proteins possessing other immunological properties...
  40. pmc Evaluation of a 2-pyridone, KRQ-10018, against Mycobacterium tuberculosis in vitro and in vivo
    Anne J Lenaerts
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Antimicrob Agents Chemother 52:1513-5. 2008
    ..tuberculosis versus moxifloxacin. In vivo efficacy of KRQ-10018 at 300 mg/kg of body weight was similar to that of isoniazid at 25 mg/kg, but showed less activity than moxifloxacin at 300 mg/kg...
  41. pmc Increased expression of host iron-binding proteins precedes iron accumulation and calcification of primary lung lesions in experimental tuberculosis in the guinea pig
    Randall J Basaraba
    Department of Microbiology, Immunology and Pathology, 1619 Campus Delivery, Colorado State University, Fort Collins, CO 80523 1619, USA
    Tuberculosis (Edinb) 88:69-79. 2008
    ....
  42. pmc Evaluation of standard chemotherapy in the guinea pig model of tuberculosis
    Diane J Ordway
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
    Antimicrob Agents Chemother 54:1820-33. 2010
    ..Chemotherapy also prevented the emergence in lung tissues of high levels of interleukin-10 and Foxp3-positive cells, known markers of regulatory T cells...
  43. pmc Rapid microbiologic and pharmacologic evaluation of experimental compounds against Mycobacterium tuberculosis
    Veronica Gruppo
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Antimicrob Agents Chemother 50:1245-50. 2006
    ....
  44. pmc Pulmonary lymphatics are primary sites of Mycobacterium tuberculosis infection in guinea pigs infected by aerosol
    Randall J Basaraba
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1619, USA
    Infect Immun 74:5397-401. 2006
    ..This observation suggests that in addition to being a direct conduit from the lungs to the regional lymph nodes, pulmonary lymphatics are themselves sites of infection and could be the site of latent infection...
  45. pmc Phenotypic definition of effector and memory T-lymphocyte subsets in mice chronically infected with Mycobacterium tuberculosis
    Marcela I Henao-Tamayo
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80524, USA
    Clin Vaccine Immunol 17:618-25. 2010
    ....
  46. ncbi request reprint Florid pulmonary inflammatory responses in mice vaccinated with Antigen-85 pulsed dendritic cells and challenged by aerosol with Mycobacterium tuberculosis
    Mercedes Gonzalez-Juarrero
    Mycobacteria Research Laboratories, Department of Microbiology, Pathology and Immunology, Colorado State University, Fort Collins, CO 80523, USA
    Cell Immunol 220:13-9. 2002
    ..tuberculosis infection than mice immunized with LDC pulsed with an irrelevant protein. Instead, the potent inflammatory response in the LDC-Ag85 resulted in serious consolidation of the lung tissue...
  47. doi request reprint Clinical strains of Mycobacterium tuberculosis display a wide range of virulence in guinea pigs
    Gopinath S Palanisamy
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
    Tuberculosis (Edinb) 89:203-9. 2009
    ..The use of appropriate animal models allows for this important question to be addressed...
  48. pmc Location of persisting mycobacteria in a Guinea pig model of tuberculosis revealed by r207910
    Anne J Lenaerts
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Antimicrob Agents Chemother 51:3338-45. 2007
    ..These results show that this acellular rim may, therefore, be a primary location of persisting bacilli withstanding drug treatment...
  49. ncbi request reprint The hypervirulent Mycobacterium tuberculosis strain HN878 induces a potent TH1 response followed by rapid down-regulation
    Diane Ordway
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    J Immunol 179:522-31. 2007
    ..This association may explain the paradoxical initial emergence of a TH1 response in these mice but their relatively short time of survival...
  50. pmc Disseminated disease severity as a measure of virulence of Mycobacterium tuberculosis in the guinea pig model
    Gopinath S Palanisamy
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
    Tuberculosis (Edinb) 88:295-306. 2008
    ....
  51. pmc A mutant of Mycobacterium tuberculosis lacking the 19-kDa lipoprotein Rv3763 is highly attenuated in vivo but retains potent vaccinogenic properties
    Marcela Henao-Tamayo
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80524, USA
    Vaccine 25:7153-9. 2007
    ..These data show that despite being highly attenuated, the Delta 19 mutant strongly retained vaccinogenic properties...
  52. ncbi request reprint Lymphadenitis as a major element of disease in the guinea pig model of tuberculosis
    Randall J Basaraba
    Mycobacteria Research Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Tuberculosis (Edinb) 86:386-94. 2006
    ..We discuss here the observation that the distribution and progression of lung and lymph node lesions in the guinea pig aerosol model of tuberculosis have considerable similarity to the naturally occurring disease in children...
  53. ncbi request reprint Influence of increased age on the development of herpes stromal keratitis
    Joanne Turner
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Exp Gerontol 38:1205-12. 2003
    ..We hypothesize that age-related changes in the immune response may predispose adult animals to HSK disease...
  54. ncbi request reprint NK cells respond to pulmonary infection with Mycobacterium tuberculosis, but play a minimal role in protection
    Ana Paula Junqueira-Kipnis
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    J Immunol 171:6039-45. 2003
    ....
  55. pmc Stable T-cell population expressing an effector cell surface phenotype in the lungs of mice chronically infected with Mycobacterium tuberculosis
    Ana Paula Junqueira-Kipnis
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Infect Immun 72:570-5. 2004
    ....
  56. pmc Rapid accumulation of eosinophils in lung lesions in guinea pigs infected with Mycobacterium tuberculosis
    Todd M Lasco
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Infect Immun 72:1147-9. 2004
    ..It is possible that the rapid influx of these cells, and their subsequent degranulation during acute pulmonary tuberculosis, may play a key role in the susceptibility of this animal model...
  57. ncbi request reprint Dynamics of macrophage cell populations during murine pulmonary tuberculosis
    Mercedes Gonzalez-Juarrero
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    J Immunol 171:3128-35. 2003
    ..This analytical approach may facilitate the further characterization of macrophage populations entering into the lung tissues and their relative contributions to host resistance to tuberculosis infection...
  58. pmc Rapid in vivo screening of experimental drugs for tuberculosis using gamma interferon gene-disrupted mice
    Anne J M Lenaerts
    Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523, USA
    Antimicrob Agents Chemother 47:783-5. 2003
    ..To validate this model, several fluoroquinolones, including ciprofloxacin, levofloxacin, moxifloxacin, and gatifloxacin, were tested in parallel...
  59. pmc Role for matrix metalloproteinase 9 in granuloma formation during pulmonary Mycobacterium tuberculosis infection
    Jennifer L Taylor
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, 200 West Lake Street, 1682 Campus Delivery, Fort Collins, CO 80523 1682, USA
    Infect Immun 74:6135-44. 2006
    ....
  60. ncbi request reprint Disruption of granulocyte macrophage-colony stimulating factor production in the lungs severely affects the ability of mice to control Mycobacterium tuberculosis infection
    Mercedes Gonzalez-Juarrero
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, 80523, USA
    J Leukoc Biol 77:914-22. 2005
    ..tuberculosis bacterial growth...
  61. pmc Preclinical testing of the nitroimidazopyran PA-824 for activity against Mycobacterium tuberculosis in a series of in vitro and in vivo models
    Anne J Lenaerts
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Antimicrob Agents Chemother 49:2294-301. 2005
    ..These data indicate that there is significant potential for effective oral delivery of PA-824 for the treatment of tuberculosis...
  62. ncbi request reprint Significant increases in the levels of liver enzymes in mice treated with anti-tuberculosis drugs
    Anne J Lenaerts
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Int J Antimicrob Agents 26:152-8. 2005
    ..The data obtained indicate that changes in serum enzyme levels in mice after extensive exposure to tuberculosis drugs could be useful as an initial indicator of drug-related hepatotoxicity...
  63. ncbi request reprint Foamy macrophages within lung granulomas of mice infected with Mycobacterium tuberculosis express molecules characteristic of dendritic cells and antiapoptotic markers of the TNF receptor-associated factor family
    Diane Ordway
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    J Immunol 175:3873-81. 2005
    ..These data indicate that in addition to the central role of DCs in initiating the acquired immune response against M. tuberculosis infection, they also participate in the granulomatous response...
  64. pmc Old mice express a transient early resistance to pulmonary tuberculosis that is mediated by CD8 T cells
    Joanne Turner
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins 80523, USA
    Infect Immun 70:4628-37. 2002
    ..tuberculosis...
  65. pmc Oral therapy using nanoparticle-encapsulated antituberculosis drugs in guinea pigs infected with Mycobacterium tuberculosis
    Christine M Johnson
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, 80523 1682, USA
    Antimicrob Agents Chemother 49:4335-8. 2005
    ..Both treatments significantly reduced the bacterial count and lung histopathology, suggesting that the nanoparticle drug delivery system has potential in intermitted treatment of tuberculosis...
  66. ncbi request reprint Decreased survival of guinea pigs infected with Mycobacterium tuberculosis after multiple BCG vaccinations
    Randall J Basaraba
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Vaccine 24:280-6. 2006
    ..These data indicate that multiple BCG vaccination, which is akin to super-infection with the living vaccine, leads to major organ failure pathology...
  67. pmc Tracking antigen-specific CD8 T lymphocytes in the lungs of mice vaccinated with the Mtb72F polyprotein
    Scott M Irwin
    Mycobacterial Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, 1682 Campus Delivery, Fort Collins, CO 80523 1682, USA
    Infect Immun 73:5809-16. 2005
    ..These data support the hypothesis that M. tuberculosis-specific CD8 T cells can be targeted by vaccination with the Mtb72F polyprotein...
  68. pmc Activities of TMC207, rifampin, and pyrazinamide against Mycobacterium tuberculosis infection in guinea pigs
    Shaobin Shang
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Antimicrob Agents Chemother 55:124-31. 2011
    ..These data indicate that TMC207 could be a useful addition to current treatment regimens for tuberculosis...
  69. ncbi request reprint Identification of altered integrin alpha/beta chain expression on T cells from old mice infected with Mycobacterium tuberculosis
    Joanne Turner
    Mycobacterial Research Laboratories, Department of Microbiology, Colorado State University, Fort Collins 80523, USA
    Exp Gerontol 37:907-16. 2002
    ..These findings are consistent with the hypothesis that age-associated changes occur in the number of cells that express molecules that allow T cells to traffic to inflammatory sites...
  70. ncbi request reprint Increased neutrophil influx but no impairment of protective immunity to tuberculosis in mice lacking the CD44 molecule
    Andre Kipnis
    Department of Microbiology, Immunology and Pathology, Colorado State University, 200 West Lake St, 1682 Campus Delivery, Fort Collins, CO 80523 1682, USA
    J Leukoc Biol 74:992-7. 2003
    ..These data indicate that loss of CD44 expression does not alter expression of T helper cell type 1 immunity to tuberculosis in the lungs but has major effects on the overall cellular composition of the immunopathological response...
  71. ncbi request reprint Enhanced macrophage activity in granulomatous lesions of immune mice challenged with Mycobacterium tuberculosis
    Diane Ordway
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    J Immunol 176:4931-9. 2006
    ..tuberculosis by aerosol exposure, immune mice develop a protective granulomatous lesion by increasing macrophage numbers and reduced expression of protective and inflammatory cytokines...
  72. pmc Influence of Mycobacterium bovis BCG vaccination on cellular immune response of guinea pigs challenged with Mycobacterium tuberculosis
    Diane Ordway
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
    Clin Vaccine Immunol 15:1248-58. 2008
    ....
  73. ncbi request reprint Statistical limitations to the Cornell model of latent tuberculosis infection for the study of relapse rates
    Anne J Lenaerts
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Tuberculosis (Edinb) 84:361-4. 2004
    ..When the objective of the experiment is confirmation of an effect through establishment of statistical significance, power calculations are critical in order to assure that the sample size will be sufficient to meet that objective...
  74. doi request reprint Animal model of Mycobacterium abscessus lung infection
    Diane Ordway
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
    J Leukoc Biol 83:1502-11. 2008
    ..In conclusion, IFN-gamma is critically important in the host defense against M. abscessus. As the number of effective drugs against M. abscessus is limited, the GKO mice provide a model for in vivo testing of novel drugs...
  75. ncbi request reprint Mouse and guinea pig models for testing new tuberculosis vaccines
    Ian M Orme
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Tuberculosis (Edinb) 85:13-7. 2005
    ..These include Mtb72F, a polyprotein vaccine that gives excellent protection and also boosts and prolongs the protective effect of the BCG vaccine...
  76. ncbi request reprint In vivo IL-10 production reactivates chronic pulmonary tuberculosis in C57BL/6 mice
    Joanne Turner
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins 80523, USA
    J Immunol 169:6343-51. 2002
    ..These data support the hypothesis that IL-10 plays a pivotal role during the chronic/latent stage of pulmonary tuberculosis, with increased production playing a potentially central role in promoting reactivation tuberculosis...
  77. pmc Factors associated with severe granulomatous pneumonia in Mycobacterium tuberculosis-infected mice vaccinated therapeutically with hsp65 DNA
    Jennifer L Taylor
    Department of Microbiology, Immunology and Pathology, Colorado State University, 200 West Lake Street, 1682 Campus Delivery, Fort Collins, CO 80523 1682, USA
    Infect Immun 73:5189-93. 2005
    ..This was probably an anti-inflammatory response, since lung pathology was dramatically worsened in B-cell gene-disrupted mice...
  78. ncbi request reprint The cellular immune response to Mycobacterium tuberculosis infection in the guinea pig
    Diane Ordway
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    J Immunol 179:2532-41. 2007
    ..These data reveal new information about the cellular phenotypes which mediate protective immunity or host immunopathogenesis during M. tuberculosis infection in this key animal model...
  79. ncbi request reprint Adaptive immunity to mycobacteria
    Ian Orme
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Curr Opin Microbiol 7:58-61. 2004
    ..Despite this, there are still several elements of the adaptive response that remain poorly understood...
  80. pmc Sulfolipid deficiency does not affect the virulence of Mycobacterium tuberculosis H37Rv in mice and guinea pigs
    Cecile Rousseau
    Unite de Genetique Mycobacterienne, Institut Pasteur, Paris, France
    Infect Immun 71:4684-90. 2003
    ..In the present study, we show that against all expectations, sulfolipid deficiency does not significantly affect the replication, persistence, and pathogenicity of M. tuberculosis H37Rv in mice and guinea pigs or in cultured macrophages...
  81. ncbi request reprint The expression of early resistance to an infection with Mycobacterium tuberculosis by old mice is dependent on IFN type II (IFN-gamma) but not IFN type I
    Joanne Turner
    Department of Internal Medicine, Center for Microbial Interface Biology, The Ohio State University, Columbus, OH 43210, USA
    Mech Ageing Dev 125:1-9. 2004
    ....
  82. ncbi request reprint The principal sigma factor sigA mediates enhanced growth of Mycobacterium tuberculosis in vivo
    Shiping Wu
    Department of Microbiology and Immunology, Center for Pulmonary and Infectious Disease Control, University of Texas Health Center, 11937 US Highway 271, Tyler, TX, USA
    Mol Microbiol 51:1551-62. 2004
    ..This effect may be mediated in part by increased resistance to reactive oxygen intermediates...
  83. ncbi request reprint Differential immune responses and protective efficacy induced by components of a tuberculosis polyprotein vaccine, Mtb72F, delivered as naked DNA or recombinant protein
    Yasir A W Skeiky
    Corixa Corp, Seattle, WA 98104, USA
    J Immunol 172:7618-28. 2004
    ..tuberculosis comparable to bacillus Calmette-Guérin immunization. Mtb72F in AS02A formulation is currently in phase I clinical trial, making it the first recombinant tuberculosis vaccine to be tested in humans...
  84. pmc Use of specific rRNA oligonucleotide probes for microscopic detection of Mycobacterium tuberculosis in culture and tissue specimens
    Allison L St Amand
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    J Clin Microbiol 43:5369-71. 2005
    ..We report a rapid and reliable in situ hybridization technique using rRNA oligonucleotide probes for the identification of M. tuberculosis in tissues and cultures...
  85. ncbi request reprint Protection of mice and guinea pigs against tuberculosis induced by immunization with a single Mycobacterium tuberculosis recombinant antigen, MTB41
    Yasir A W Skeiky
    Corixa Corporation, Seattle, WA, USA
    Vaccine 23:3937-45. 2005
    ....
  86. pmc Enhanced priming of adaptive immunity by a proapoptotic mutant of Mycobacterium tuberculosis
    Joseph Hinchey
    Department of Microbiology and Immunology and Howard Hughes Medical Institute, Albert Einstein College of Medicine, New York, New York 10461, USA
    J Clin Invest 117:2279-88. 2007
    ..bovis bacille Calmette-Guérin vaccination. Our results define a mechanism for a key immune evasion strategy of M. tuberculosis and provide what we believe to be a novel approach for improving mycobacterial vaccines...
  87. pmc Characterization of the protective T-cell response generated in CD4-deficient mice by a live attenuated Mycobacterium tuberculosis vaccine
    Steven C Derrick
    Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    Immunology 120:192-206. 2007
    ....
  88. pmc Mice fed lipid-encapsulated Mycobacterium bovis BCG are protected against aerosol challenge with Mycobacterium tuberculosis
    Frank E Aldwell
    Department of Microbiology and Immunology, University of Otago, P O Box 56, Dunedin, New Zealand
    Infect Immun 73:1903-5. 2005
    ..These results are consistent with the induction of tuberculin-specific cell-mediated immune responses...
  89. ncbi request reprint The study of Mycobacterium leprae infection in interferon-gamma gene--disrupted mice as a model to explore the immunopathologic spectrum of leprosy
    Linda B Adams
    National Hansen s Disease Programs, Laboratory Research Branch, Louisiana State University, Baton Rouge, Louisiana 70803, USA
    J Infect Dis 185:S1-8. 2002
    ..Thus, although deficient in an important Th1 cytokine, GKO mice possess compensatory mechanisms to control M. leprae growth and feature elements resembling mid-borderline leprosy in humans...

Research Grants48

  1. DEFINED NATIVE ANTIGENS AND IMMUNITY TO TUBERCULOSIS
    Ian Orme; Fiscal Year: 2006
    ..The proposed work will draw upon the broad expertise of various members of the Mycobacteria Research Laboratories at Colorado State University, as well as several eminent consultants and advisers. ..
  2. Guinea pig model for TB vaccine evaluations
    Ian Orme; Fiscal Year: 2007
    ....
  3. Chronic Tuberculosis: Latent or Dynamic
    Ian Orme; Fiscal Year: 2007
    ....
  4. CHRONIC TUBERCULOSIS--LATENT OR DYNAMIC
    Ian Orme; Fiscal Year: 2003
    ..In this latter endeavor we shall be assisted by highly qualified mycobacterial chemists from within the Mycobacteria Research Laboratories [MRL] at CSU. ..
  5. AGING AND IMMUNITY IN TUBERCULOSIS
    Ian Orme; Fiscal Year: 2002
    ....
  6. STRATEGIES FOR TUBERCULOSIS VACCINE DEVELOPMENT AND SCRE
    Ian Orme; Fiscal Year: 2004
    ..abstract_text> ..
  7. Response Therapies for MDR-TB
    Ian Orme; Fiscal Year: 2007
    ..Safety testing issues, toxicology testing, and process development leading to GMP production are also planned for the later stages of this proposed program. ..
  8. AGING AND IMMUNITY TO TUBERCULOSIS
    Ian Orme; Fiscal Year: 1993
    ..Using data gathered by these diverse procedures, it is anticipated that new important knowledge will be gained regarding the precise events which occur in the aged animal exposed to virulent pulmonary tuberculosis...
  9. DEFINED NATIVE ANTIGENS AND IMMUNITY TO TUBERCULOSIS
    Ian Orme; Fiscal Year: 2000
    ..As previously in this Program, the proposed work will draw upon the broad expertise of various members of the Mycobacteria Research Laboratories, CSU, as well as a number of highly qualified consultants/collaborators. ..