Ken E Olson

Summary

Affiliation: Colorado State University
Country: USA

Publications

  1. ncbi request reprint Development of a Sindbis virus expression system that efficiently expresses green fluorescent protein in midguts of Aedes aegypti following per os infection
    K E Olson
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Colorado State University, Ft Collins, CO 80523, USA
    Insect Mol Biol 9:57-65. 2000
  2. ncbi request reprint Developing arbovirus resistance in mosquitoes
    Ken E Olson
    Arthropod borne and Infectious Diseases Laboratory AIDL, Department of Microbiology, Colorado State University, Fort Collins, CO 80523, USA
    Insect Biochem Mol Biol 32:1333-43. 2002
  3. pmc Engineering RNA interference-based resistance to dengue virus type 2 in genetically modified Aedes aegypti
    Alexander W E Franz
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Proc Natl Acad Sci U S A 103:4198-203. 2006
  4. ncbi request reprint Using RNA interference to develop dengue virus resistance in genetically modified Aedes aegypti
    Emily A Travanty
    Department of Microbiology, Immunology and Pathology, Arthropod Borne and Infectious Diseases Laboratory, Colorado State University, Foothills Research Campus, Fort Collins, CO 80523, USA
    Insect Biochem Mol Biol 34:607-13. 2004
  5. pmc Suppression of RNA interference increases alphavirus replication and virus-associated mortality in Aedes aegypti mosquitoes
    Chris M Cirimotich
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    BMC Microbiol 9:49. 2009
  6. pmc Dengue virus type 2 infections of Aedes aegypti are modulated by the mosquito's RNA interference pathway
    Irma Sanchez-Vargas
    Department of Microbiology, Arthropod Borne and Infectious Diseases Laboratory, Colorado State University, Fort Collins, Colorado, United States of America
    PLoS Pathog 5:e1000299. 2009
  7. ncbi request reprint RNA interference, arthropod-borne viruses, and mosquitoes
    Irma Sanchez-Vargas
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Virus Res 102:65-74. 2004
  8. ncbi request reprint Variation in vector competence for dengue 2 virus among 24 collections of Aedes aegypti from Mexico and the United States
    Kristine E Bennett
    Department of Microbiology, Colorado State University, Fort Collins 80523, USA
    Am J Trop Med Hyg 67:85-92. 2002
  9. ncbi request reprint Infectious clone construction of dengue virus type 2, strain Jamaican 1409, and characterization of a conditional E6 mutation
    Dennis J Pierro
    Arthropod Borne and Infectious Disease Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Foothills Research Campus, Fort Collins, CO 80523, USA
    J Gen Virol 87:2263-8. 2006
  10. pmc Dengue virus type 2: replication and tropisms in orally infected Aedes aegypti mosquitoes
    Ma Isabel Salazar
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523 1692, USA
    BMC Microbiol 7:9. 2007

Collaborators

Detail Information

Publications41

  1. ncbi request reprint Development of a Sindbis virus expression system that efficiently expresses green fluorescent protein in midguts of Aedes aegypti following per os infection
    K E Olson
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Colorado State University, Ft Collins, CO 80523, USA
    Insect Mol Biol 9:57-65. 2000
    ..The MRE3'2 J dsSIN expression system should be an important tool for efficient gene expression in Ae. aegypti midguts...
  2. ncbi request reprint Developing arbovirus resistance in mosquitoes
    Ken E Olson
    Arthropod borne and Infectious Diseases Laboratory AIDL, Department of Microbiology, Colorado State University, Fort Collins, CO 80523, USA
    Insect Biochem Mol Biol 32:1333-43. 2002
    ..We are now developing virus-resistant mosquito lines by transformation with transposable elements that express effector RNAs from mosquito-active promoters...
  3. pmc Engineering RNA interference-based resistance to dengue virus type 2 in genetically modified Aedes aegypti
    Alexander W E Franz
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Proc Natl Acad Sci U S A 103:4198-203. 2006
    ..Engineering of transgenic A. aegypti that show a high level of resistance against DENV-2 provides a powerful tool for developing population replacement strategies to control transmission of dengue viruses...
  4. ncbi request reprint Using RNA interference to develop dengue virus resistance in genetically modified Aedes aegypti
    Emily A Travanty
    Department of Microbiology, Immunology and Pathology, Arthropod Borne and Infectious Diseases Laboratory, Colorado State University, Foothills Research Campus, Fort Collins, CO 80523, USA
    Insect Biochem Mol Biol 34:607-13. 2004
    ..If successful, this strategy has the advantage of harnessing a naturally occurring vector response to block DENV infection in a mosquito vector and profoundly affect virus transmission...
  5. pmc Suppression of RNA interference increases alphavirus replication and virus-associated mortality in Aedes aegypti mosquitoes
    Chris M Cirimotich
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    BMC Microbiol 9:49. 2009
    ....
  6. pmc Dengue virus type 2 infections of Aedes aegypti are modulated by the mosquito's RNA interference pathway
    Irma Sanchez-Vargas
    Department of Microbiology, Arthropod Borne and Infectious Diseases Laboratory, Colorado State University, Fort Collins, Colorado, United States of America
    PLoS Pathog 5:e1000299. 2009
    ..Our findings indicate a major role for RNAi as a determinant of DENV transmission by Ae. aegypti...
  7. ncbi request reprint RNA interference, arthropod-borne viruses, and mosquitoes
    Irma Sanchez-Vargas
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Virus Res 102:65-74. 2004
    ..Towards this goal, we are developing transgenic A. aegypti lines that are refractory to DENV by exploiting the RNAi pathway...
  8. ncbi request reprint Variation in vector competence for dengue 2 virus among 24 collections of Aedes aegypti from Mexico and the United States
    Kristine E Bennett
    Department of Microbiology, Colorado State University, Fort Collins 80523, USA
    Am J Trop Med Hyg 67:85-92. 2002
    ..The MIB and MEB were not completely independent as determined by regression analysis. Midgut infection rates were dose dependent...
  9. ncbi request reprint Infectious clone construction of dengue virus type 2, strain Jamaican 1409, and characterization of a conditional E6 mutation
    Dennis J Pierro
    Arthropod Borne and Infectious Disease Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Foothills Research Campus, Fort Collins, CO 80523, USA
    J Gen Virol 87:2263-8. 2006
    ..However, in vitro cell culture-derived growth-curve data and in vivo mosquito-infection rates revealed that none of the analysed DENV-2 strains differed from each other...
  10. pmc Dengue virus type 2: replication and tropisms in orally infected Aedes aegypti mosquitoes
    Ma Isabel Salazar
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523 1692, USA
    BMC Microbiol 7:9. 2007
    ..Here we document the virogenesis of DENV-2 in newly-colonized Aedes aegypti mosquitoes from Chetumal, Mexico in order to understand better the effect of vector-virus interactions on dengue transmission...
  11. pmc Aedes aegypti uses RNA interference in defense against Sindbis virus infection
    Corey L Campbell
    Arthropod borne Infectious Diseases Laboratory Microbiology, Immunology, and Pathology Department, Colorado State University, Fort Collins, USA
    BMC Microbiol 8:47. 2008
    ..The goal of this study was to use Ae. aegypti as a model system to determine how the mosquito's anti-viral RNAi pathway interacts with recombinant Sindbis virus (SINV; family Togaviridae, genus Alphavirus)...
  12. pmc RNA interference acts as a natural antiviral response to O'nyong-nyong virus (Alphavirus; Togaviridae) infection of Anopheles gambiae
    Kimberly M Keene
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Proc Natl Acad Sci U S A 101:17240-5. 2004
    ..These observations provide direct evidence that RNAi is an antagonist of ONNV replication in A. gambiae, and they suggest that the innate immune response conditions vector competence...
  13. pmc Genetic determinants of Sindbis virus mosquito infection are associated with a highly conserved alphavirus and flavivirus envelope sequence
    Dennis J Pierro
    Arthropod Borne and Infectious Disease Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    J Virol 82:2966-74. 2008
    ..In addition, a unique PPF/.GDS amino acid motif was located between these two sites that was found to be a highly conserved sequence among alphaviruses and flaviviruses but not other arboviruses...
  14. pmc Molecular determinants of mouse neurovirulence and mosquito infection for Western equine encephalitis virus
    Eric C Mossel
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America
    PLoS ONE 8:e60427. 2013
    ....
  15. pmc The neovolcanic axis is a barrier to gene flow among Aedes aegypti populations in Mexico that differ in vector competence for Dengue 2 virus
    Saul Lozano-Fuentes
    Department of Microbiology, Colorado State University, Fort Collins, Colorado, United States of America
    PLoS Negl Trop Dis 3:e468. 2009
    ..Previous population genetic and vector competence studies have demonstrated substantial genetic structure and major differences in the ability to transmit dengue viruses in Ae. aegypti populations in Mexico...
  16. pmc RNA silencing of dengue virus type 2 replication in transformed C6/36 mosquito cells transcribing an inverted-repeat RNA derived from the virus genome
    Zach N Adelman
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Foothills Research Campus, Colorado State University, Fort Collins, CO 80523, USA
    J Virol 76:12925-33. 2002
    ..These observations were consistent with RNA silencing as the mechanism of resistance to DEN-2 in transformed mosquito cells...
  17. pmc Treatment with cationic liposome-DNA complexes (CLDCs) protects mice from lethal Western equine encephalitis virus (WEEV) challenge
    Christopher H Logue
    Division of Vector Borne Infectious Diseases, Centers for Disease Control, Fort Collins, CO 80521, USA
    Antiviral Res 87:195-203. 2010
    ....
  18. ncbi request reprint Genetic determinants of Sindbis virus strain TR339 affecting midgut infection in the mosquito Aedes aegypti
    Dennis J Pierro
    Arthropod Borne and Infectious Disease Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    J Gen Virol 88:1545-54. 2007
    ..In addition, SINV propagated in a mammalian cell line had a significantly lower A. aegypti midgut 50 % infectious dose than virus propagated in a mosquito cell line...
  19. pmc Rapid intraspecific evolution of miRNA and siRNA genes in the mosquito Aedes aegypti
    Scott A Bernhardt
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America
    PLoS ONE 7:e44198. 2012
    ..Furthermore, refractoriness of mosquitoes to infection with dengue virus was significantly positively correlated for nucleotide diversity indices in dicer-2...
  20. pmc Comparison of dengue virus type 2-specific small RNAs from RNA interference-competent and -incompetent mosquito cells
    Jaclyn C Scott
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA
    PLoS Negl Trop Dis 4:e848. 2010
    ....
  21. pmc The RNA interference pathway affects midgut infection- and escape barriers for Sindbis virus in Aedes aegypti
    Cynthia C H Khoo
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    BMC Microbiol 10:130. 2010
    ..We used these mosquitoes to reveal effects of RNAi impairment in the midgut on SINV replication, midgut infection and dissemination efficiencies, and mosquito longevity...
  22. pmc Expression profiling and comparative analyses of seven midgut serine proteases from the yellow fever mosquito, Aedes aegypti
    Doug E Brackney
    Arthropod Infectious Disease Laboratory, 1692 Campus Delivery, Department of Microbiology, Immunology and Pathology at Colorado State University, Fort Collins, CO 80523, USA
    J Insect Physiol 56:736-44. 2010
    ..A subfamily of midgut serine proteases and/or that A. aegypti has evolved an array of proteases with a broad range of substrate specificities for rapid, efficient digestion of bloodmeals...
  23. pmc The effects of midgut serine proteases on dengue virus type 2 infectivity of Aedes aegypti
    Doug E Brackney
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Am J Trop Med Hyg 79:267-74. 2008
    ..These results suggest that some midgut serine proteases may actually limit DENV-2 infectivity of Ae. aegypti...
  24. pmc Liposome-antigen-nucleic acid complexes protect mice from lethal challenge with western and eastern equine encephalitis viruses
    Aaron T Phillips
    Arthropod Borne and Infectious Disease Laboratory, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, USA
    J Virol 88:1771-80. 2014
    ..We discuss plausible mechanisms as well the potential utility of our LANAC formulation as a pan-alphavirus vaccine. ..
  25. ncbi request reprint Genetic variation within the premembrane coding region of dengue viruses from the Yucatan peninsula of Mexico
    Francisco J Diaz
    Department of Microbiology, Colorado State University, Fort Collins 80523, USA
    Am J Trop Med Hyg 67:93-101. 2002
    ..One DEN-2 isolate from 1996 was demonstrated to cluster with viruses of the Sri Lanka genotype, none of which have been detected before in the Americas...
  26. doi request reprint Alphavirus transducing systems
    Brian D Foy
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Adv Exp Med Biol 627:19-34. 2008
    ..These virus-based expression systems also facilitate the researcher's ability to decide which gene-based disease control strategies merit a further investment in time and resources in transgenic mosquitoes...
  27. pmc Deletions in the putative cell receptor-binding domain of Sindbis virus strain MRE16 E2 glycoprotein reduce midgut infectivity in Aedes aegypti
    Kevin M Myles
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80526, USA
    J Virol 77:8872-81. 2003
    ..0 log(10) PFU/ml) in mosquitoes 4 days after infection by intrathoracic inoculation. These results suggest that the deleted portion of the E2 CRBD represents an important determinant of MRE16 virus midgut infectivity in A. aegypti...
  28. ncbi request reprint Comparison of the transmission potential of two genetically distinct Sindbis viruses after oral infection of Aedes aegypti (Diptera: Culicidae)
    Kevin M Myles
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    J Med Entomol 41:95-106. 2004
    ..MRE16 and TR339 viruses now represent a well-characterized model system for the further study of virus determinants of vector infection, particularly determinants affecting the midgut escape barrier in Ae. aegypti...
  29. pmc Infectious alphavirus production from a simple plasmid transfection+
    J Jordan Steel
    Department of Microbiology, Immunology, and Pathology, 1682 Campus Delivery, Colorado State University, Fort Collins, CO 80523, USA
    Virol J 8:356. 2011
    ..This system represents a more efficient method for producing recombinant Sindbis viruses...
  30. pmc Virulence variation among isolates of western equine encephalitis virus in an outbred mouse model
    Christopher H Logue
    Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA
    J Gen Virol 90:1848-58. 2009
    ....
  31. pmc Small RNA profiling of Dengue virus-mosquito interactions implicates the PIWI RNA pathway in anti-viral defense
    Ann M Hess
    Department of Statistics, Colorado State University, Fort Collins, Colorado 80523, USA
    BMC Microbiol 11:45. 2011
    ..aegypti, we deep-sequenced small non-coding RNAs. Triplicate biological replicates were used so that rigorous statistical metrics could be applied...
  32. pmc Subgenomic reporter RNA system for detection of alphavirus infection in mosquitoes
    J Jordan Steel
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, United States of America
    PLoS ONE 8:e84930. 2013
    ..This virus inducible reporter system demonstrates a novel approach for detecting non-recombinant virus infection in mosquito cell culture and in live transgenic mosquitoes. ..
  33. pmc Bioluminescent imaging and histopathologic characterization of WEEV neuroinvasion in outbred CD-1 mice
    Aaron T Phillips
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, United States of America
    PLoS ONE 8:e53462. 2013
    ..BLM provides a quantifiable measure of alphaviral neural disease progression and a method for evaluating antiviral strategies...
  34. ncbi request reprint Flavivirus susceptibility in Aedes aegypti
    William C Black
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Arch Med Res 33:379-88. 2002
    ..We end with a discussion of approaches being taken to identify the genes that may control flavivirus susceptibility in Ae. aegypti...
  35. pmc Recombination-ready Sindbis replicon expression vectors for transgene expression
    Brian J Geiss
    Arthropod Borne and Infectious Diseases Laboratory, Department of Molecular Biology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Virol J 4:112. 2007
    ....
  36. pmc Use of Sindbis virus-mediated RNA interference to demonstrate a conserved role of Broad-Complex in insect metamorphosis
    Mirka Uhlirova
    Department of Molecular Biology, University of South Bohemia and Institute of Entomology, The Czech Academy of Sciences, Ceske Budejovice, 37005 Czech Republic
    Proc Natl Acad Sci U S A 100:15607-12. 2003
    ..We also demonstrate that the Sindbis virus is a useful vehicle for silencing of developmental genes in new insect models...
  37. ncbi request reprint Formation and loss of large, unstable tandem arrays of the piggyBac transposable element in the yellow fever mosquito, Aedes aegypti
    Zach N Adelman
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697 3900, USA
    Transgenic Res 13:411-25. 2004
    ....
  38. ncbi request reprint Sindbis virus induces transport processes and alters expression of innate immunity pathway genes in the midgut of the disease vector, Aedes aegypti
    Heather R Sanders
    Department of Cell Biology and Neuroscience, University of California, Riverside, California 92521, USA
    Insect Biochem Mol Biol 35:1293-307. 2005
    ..In addition to these observations, transcript changes were observed in infected mosquitoes that suggested involvement of Toll and c-jun amino terminal kinase immune cascades as a response to viral infection...
  39. ncbi request reprint Introduction of the American/Asian genotype of dengue 2 virus into the Yucatan State of Mexico
    Maria A Lorono-Pino
    Laboratorio de Arbovirologia, Centro de Investigaciones Regionales Dr Hideyo Noguchi, Universidad Autonoma de Yucatan, Av Itzaes No 490 x 59, Centro, Merida, Yucatan, Mexico 97000
    Am J Trop Med Hyg 71:485-92. 2004
    ..Of these, 87 (31%) patients met the World Health Organization criteria for dengue hemorrhagic fever, including two patients who died. The majority (77%) of the patients experienced secondary infections in this epidemic...
  40. pmc La Crosse bunyavirus nonstructural protein NSs serves to suppress the type I interferon system of mammalian hosts
    Gjon Blakqori
    Department of Virology, University of Freiburg, Hermann Herder Str 11, Freiburg D 79008, Germany
    J Virol 81:4991-9. 2007
    ..Since deletion of the viral NSs gene can be fully complemented by inactivation of the host's IFN system, we propose that the major biological function of NSs is suppression of the mammalian innate immune response...
  41. pmc Annotation and expression profiling of apoptosis-related genes in the yellow fever mosquito, Aedes aegypti
    Bart Bryant
    Molecular, Cellular, and Developmental Biology Program, Arthropod Genomics Center, Division of Biology, Ackert Hall, Kansas State University, Manhattan, KS, USA
    Insect Biochem Mol Biol 38:331-45. 2008
    ..Expression of IMP in mosquito cells caused apoptosis, indicating that it is a functional pro-death protein. Further characterization of these genes will help elucidate the molecular mechanisms of apoptosis regulation in Ae. aegypti...