D K Gardner

Summary

Affiliation: Colorado Center for Reproductive Medicine
Country: USA

Publications

  1. ncbi request reprint Quality control in human in vitro fertilization
    David K Gardner
    Colorado Center for Reproductive Medicine, Englewood, Colorado, USA
    Semin Reprod Med 23:319-24. 2005
  2. ncbi request reprint Improved felid embryo development by group culture is maintained with heterospecific companions
    Rebecca E Spindler
    Department of Reproductive Sciences, Conservation and Research Center, Smithsonian s National Zoological Park, Front Royal, VA 22630, USA
    Theriogenology 66:82-92. 2006
  3. ncbi request reprint Dissection of culture media for embryos: the most important and less important components and characteristics
    David K Gardner
    Department of Zoology, University of Melbourne, Victoria 3010, Australia
    Reprod Fertil Dev 20:9-18. 2008
  4. ncbi request reprint Culture and transfer of viable blastocysts: a feasible proposition for human IVF
    D K Gardner
    Colorado Center for Reproductive Medicine, Englewood 80110, USA
    Hum Reprod 15:9-23. 2000
  5. ncbi request reprint Ex vivo early embryo development and effects on gene expression and imprinting
    David K Gardner
    Colorado Center for Reproductive Medicine, Englewood, 80113, USA
    Reprod Fertil Dev 17:361-70. 2005
  6. ncbi request reprint Towards a single embryo transfer
    David K Gardner
    Colorado Center for Reproductive Medicine, 799 East Hampden Avenue, Suite 520, Englewood, Colorado 80110, USA
    Reprod Biomed Online 6:470-81. 2003
  7. ncbi request reprint Noninvasive assessment of human embryo nutrient consumption as a measure of developmental potential
    D K Gardner
    Colorado Center for Reproductive Medicine, Englewood, Colorado 80110, USA
    Fertil Steril 76:1175-80. 2001
  8. ncbi request reprint Blastocyst transfer
    David K Gardner
    Colorado Center for Reproductive Medicine, Englewood 80110, USA
    Clin Obstet Gynecol 46:231-8. 2003
  9. ncbi request reprint Analysis of oocyte physiology to improve cryopreservation procedures
    David K Gardner
    Colorado Center for Reproductive Medicine, Englewood, CO 80113, USA
    Theriogenology 67:64-72. 2007
  10. ncbi request reprint Single blastocyst transfer: a prospective randomized trial
    David K Gardner
    Colorado Center for Reproductive Medicine, Englewood, Colorado 80110, USA
    Fertil Steril 81:551-5. 2004

Collaborators

Detail Information

Publications63

  1. ncbi request reprint Quality control in human in vitro fertilization
    David K Gardner
    Colorado Center for Reproductive Medicine, Englewood, Colorado, USA
    Semin Reprod Med 23:319-24. 2005
    ..It is proposed that under the appropriate conditions, the MEA is a useful adjunct to quality control in human IVF, but several assays used in concert are better than a single test...
  2. ncbi request reprint Improved felid embryo development by group culture is maintained with heterospecific companions
    Rebecca E Spindler
    Department of Reproductive Sciences, Conservation and Research Center, Smithsonian s National Zoological Park, Front Royal, VA 22630, USA
    Theriogenology 66:82-92. 2006
    ..If this phenomenon can be extrapolated across species, this may be an avenue for 'common animal embryos' to improve developmental potential of embryos from rare, unrelated taxa...
  3. ncbi request reprint Dissection of culture media for embryos: the most important and less important components and characteristics
    David K Gardner
    Department of Zoology, University of Melbourne, Victoria 3010, Australia
    Reprod Fertil Dev 20:9-18. 2008
    ..The future of embryo culture will likely be based on novel culture chips capable of providing temporal dynamics while facilitating real-time analysis of embryo physiology...
  4. ncbi request reprint Culture and transfer of viable blastocysts: a feasible proposition for human IVF
    D K Gardner
    Colorado Center for Reproductive Medicine, Englewood 80110, USA
    Hum Reprod 15:9-23. 2000
    ....
  5. ncbi request reprint Ex vivo early embryo development and effects on gene expression and imprinting
    David K Gardner
    Colorado Center for Reproductive Medicine, Englewood, 80113, USA
    Reprod Fertil Dev 17:361-70. 2005
    ..Whatever the aetiology, continued long-term monitoring of the children conceived through ART is warranted...
  6. ncbi request reprint Towards a single embryo transfer
    David K Gardner
    Colorado Center for Reproductive Medicine, 799 East Hampden Avenue, Suite 520, Englewood, Colorado 80110, USA
    Reprod Biomed Online 6:470-81. 2003
    ..Of great significance, embryos cultured in sequential media G1 and G2 have the same rate of development as embryos developed in vivo...
  7. ncbi request reprint Noninvasive assessment of human embryo nutrient consumption as a measure of developmental potential
    D K Gardner
    Colorado Center for Reproductive Medicine, Englewood, Colorado 80110, USA
    Fertil Steril 76:1175-80. 2001
    ..To determine the relationship between blastocyst development and morphology and embryo metabolism...
  8. ncbi request reprint Blastocyst transfer
    David K Gardner
    Colorado Center for Reproductive Medicine, Englewood 80110, USA
    Clin Obstet Gynecol 46:231-8. 2003
  9. ncbi request reprint Analysis of oocyte physiology to improve cryopreservation procedures
    David K Gardner
    Colorado Center for Reproductive Medicine, Englewood, CO 80113, USA
    Theriogenology 67:64-72. 2007
    ..Analysis of meiotic spindle dynamics and embryo development following IVF, also indicate that vitrification is less traumatic than slow freezing, and therefore has the greatest potential for successful oocyte cryopreservation...
  10. ncbi request reprint Single blastocyst transfer: a prospective randomized trial
    David K Gardner
    Colorado Center for Reproductive Medicine, Englewood, Colorado 80110, USA
    Fertil Steril 81:551-5. 2004
    ..To determine the efficacy of single blastocyst transfer...
  11. ncbi request reprint EDTA stimulates cleavage stage bovine embryo development in culture but inhibits blastocyst development and differentiation
    D K Gardner
    Colorado Center for Reproductive Medicine, Englewood, CO 80110, USA
    Mol Reprod Dev 57:256-61. 2000
    ..However, EDTA should not be used for the later stage embryo as the inhibition of glycolysis reduces energy production at the blastocyst stage and significantly inhibits inner cell mass development...
  12. ncbi request reprint Changing the start temperature and cooling rate in a slow-freezing protocol increases human blastocyst viability
    David K Gardner
    Colorado Center for Reproductive Medicine, Englewood 80110, USA
    Fertil Steril 79:407-10. 2003
    ..To determine the effect of start temperature and cooling rate of a slow freezing protocol on human blastocyst viability...
  13. ncbi request reprint Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer
    D K Gardner
    Colorado Center for Reproductive Medicine, Englewood, Colorado, USA
    Fertil Steril 73:1155-8. 2000
    ..To determine the relationship between blastocyst score and pregnancy outcome...
  14. ncbi request reprint Assessment of embryo viability: the ability to select a single embryo for transfer--a review
    D K Gardner
    Colorado Center for Reproductive Medicine, 799 East Hampden Avenue Suite 520, Englewood, CO 80110, USA
    Placenta 24:S5-12. 2003
    ..Such systems will help lead to single embryo transfers for the majority of IVF patients...
  15. ncbi request reprint Physiology and culture of the human blastocyst
    David K Gardner
    Colorado Center for Reproductive Medicine, 799 East Hampden Avenue, Suite 520, Englewood, CO 80110, USA
    J Reprod Immunol 55:85-100. 2002
    ..With the introduction of new scoring systems for the blastocyst and the non-invasive assessment of metabolic activity of individual embryos, it should be possible to move to single blastocyst transfer for the majority of patients...
  16. ncbi request reprint Inhibiting 3-phosphoglycerate kinase by EDTA stimulates the development of the cleavage stage mouse embryo
    M Lane
    Colorado Center for Reproductive Medicine, Englewood, Colorado, USA
    Mol Reprod Dev 60:233-40. 2001
    ..Therefore, the effect of EDTA appears to be as a chelator of divalent cations such as magnesium, that are required for normal activity of kinases such as 3-phosphoglycerate kinase...
  17. ncbi request reprint Vitrification of mouse oocytes using a nylon loop
    M Lane
    Colorado Center for Reproductive Medicine, Research and Development, Englewood, Colorado 80110, USA
    Mol Reprod Dev 58:342-7. 2001
    ..4 and 26.2%, respectively). Vitrification of mouse oocytes using the nylon loop results in the retention of viability of the oocytes and subsequent embryos...
  18. ncbi request reprint Blastocyst culture and transfer increases the efficiency of oocyte donation
    W B Schoolcraft
    Colorado Center for Reproductive Medicine, Englewood, Colorado 80110, USA
    Fertil Steril 74:482-6. 2000
    ..To determine the impact of blastocyst transfer on an oocyte donation program...
  19. ncbi request reprint Maintenance of the meiotic spindle during vitrification in human and mouse oocytes
    M G Larman
    Colorado Center for Reproductive Medicine, 799 East Hampden Avenue, Suite 520, Englewood, CO 80113, USA
    Reprod Biomed Online 15:692-700. 2007
    ..Using a vitrification protocol employing 37 degrees C impacts negligibly on the meiotic spindle. Thus, fertilization can proceed without having to await spindle reformation...
  20. ncbi request reprint Blastocyst versus day 2 or 3 transfer
    W B Schoolcraft
    Colorado Center for Reproductive Medicine, Englewood, Colorado 80110, USA
    Semin Reprod Med 19:259-68. 2001
    ..Rather, blastocyst transfer may be of benefit to the majority if not all patients attending for IVF...
  21. ncbi request reprint Symposium: innovative techniques in human embryo viability assessment. Can proteomics help to shape the future of human assisted conception?
    M G Katz-Jaffe
    Colorado Center for Reproductive Medicine, USA
    Reprod Biomed Online 17:497-501. 2008
    ..Furthermore, proteomics may contribute in the design of a non-invasive viability assay to assist in the selection of embryos for transfer in human assisted reproduction...
  22. doi request reprint Blastocysts from patients with polycystic ovaries exhibit altered transcriptome and secretome
    M G Katz-Jaffe
    Colorado Center for Reproductive Medicine, Lone Tree, CO 80124, USA
    Reprod Biomed Online 21:520-6. 2010
    ....
  23. ncbi request reprint Vitrification of mouse and human blastocysts using a novel cryoloop container-less technique
    M Lane
    Colorado Center for Reproductive Medicine, Englewood 80110, USA
    Fertil Steril 72:1073-8. 1999
    ..To vitrify mouse and human blastocysts with use of the cryoloop procedure and to assess subsequent development...
  24. pmc The role of proteomics in defining the human embryonic secretome
    M G Katz-Jaffe
    Colorado Center for Reproductive Medicine, Lone Tree, CO 80124, USA
    Mol Hum Reprod 15:271-7. 2009
    ..From a clinical perspective, embryo selection based on morphological assessment and non-invasive analysis of the human embryonic secretome may improve IVF success and lead to routine single embryo transfers...
  25. ncbi request reprint Effects of oxygen tension on the establishment and lactate dehydrogenase activity of murine embryonic stem cells
    J Gibbons
    Colorado Center for Reproductive Medicine, Englewood, CO, USA
    Cloning Stem Cells 8:117-22. 2006
    ..Reduced O(2) environment had significant positive effects on the establishment and maintenance of murine stem cells, supporting the hypothesis, whereas the LDH isozyme transition was consistent among treatments...
  26. doi request reprint Transcriptome analysis of in vivo and in vitro matured bovine MII oocytes
    M G Katz-Jaffe
    Colorado Center for Reproductive Medicine, Lone Tree, CO, USA
    Theriogenology 71:939-46. 2009
    ..05). Distinct IVM transcription patterns reflected the oocyte's response to its surrounding environment. Monitoring transcription levels of key oocyte maturation genes may subsequently assist in improving IVM success...
  27. pmc Effect of essential amino acids on mouse embryo viability and ammonium production
    M Lane
    Colorado Center for Reproductive Medicine, Research and Development, Englewood, Colorado, USA
    J Assist Reprod Genet 18:519-25. 2001
    ..To examine the effect of essential amino acids concentrations on mouse embryo development...
  28. ncbi request reprint 1,2-propanediol and the type of cryopreservation procedure adversely affect mouse oocyte physiology
    M G Larman
    Colorado Center for Reproductive Medicine, Englewood, CO 80133, USA
    Hum Reprod 22:250-9. 2007
    ..The aim of this work was to examine the effect of 1,2-propanediol (PrOH) and type of cryopreservation procedure (slow freezing and vitrification) on oocyte physiology...
  29. ncbi request reprint Lactate regulates pyruvate uptake and metabolism in the preimplantation mouse embryo
    M Lane
    Institute of Reproduction and Development, Monash University, Monash Medical Centre, Clayton, Victoria 3168, Australia
    Biol Reprod 62:16-22. 2000
    ..Therefore it is proposed that the differences in pyruvate and lactate metabolism at the different stages of development are due to differences in the in situ regulation of LDH by cytosolic redox potential...
  30. ncbi request reprint Embryo transfer: techniques and variables affecting success
    W B Schoolcraft
    Colorado Center for Reproductive Medicine, Englewood, Colorado 80110, USA
    Fertil Steril 76:863-70. 2001
    ....
  31. ncbi request reprint Assessment of metabolism of equine morulae and blastocysts
    M Lane
    Colorado Center for Reproductive Medicine, Englewood, Colorado 80110, USA
    Mol Reprod Dev 59:33-7. 2001
    ..However, there was a large range in nutrient consumption within the highest grade blastocysts. This suggests that nutrient uptake may be useful as a viability marker of equine blastocysts...
  32. ncbi request reprint Embryology in the era of proteomics
    M G Katz-Jaffe
    Colorado Center for Reproductive Medicine, 799 E Hampden Avenue, Suite 520, Englewood, CO 80113, USA
    Theriogenology 68:S125-30. 2007
    ....
  33. ncbi request reprint Metabolic regulation of in vitro-produced bovine embryos. II. Effects of phenazine ethosulfate, sodium azide and 2,4-dinitrophenol during post-compaction development on glucose metabolism and lipid accumulation
    Jose Fernando De La Torre-Sanchez
    Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, CO 80523 1683, USA
    Reprod Fertil Dev 18:597-607. 2006
    ..Use of PES in culture media may be a promising approach to improving in vitro production of embryos...
  34. ncbi request reprint Reduced oxygen concentration improves the developmental competence of mouse oocytes following in vitro maturation
    Kimberly A Preis
    Colorado Center for Reproductive Medicine, Englewood, Colorado 80113, USA
    Mol Reprod Dev 74:893-903. 2007
    ..These findings have implications for oocyte maturation in both clinical and research laboratories...
  35. ncbi request reprint One-step versus two-step culture of mouse preimplantation embryos
    David K Gardner
    Hum Reprod 21:1935-6; author reply 1936-9. 2006
  36. ncbi request reprint The CryoLoop facilitates re-vitrification of embryos at four successive stages of development without impairing embryo growth
    Courtney B Sheehan
    Research Department, Colorado Center for Reproductive Medicine, Englewood, CO 80113, USA
    Hum Reprod 21:2978-84. 2006
    ..This study investigated the effect of re-vitrification on mouse embryo preimplantation development and viability post-transfer...
  37. ncbi request reprint Embryo culture medium: which is the best?
    Michelle Lane
    Research Centre for Reproductive Health, Discipline of Paediatrics and Reproductive Health, University of Adelaide, Adelaide, South Australia, Australia
    Best Pract Res Clin Obstet Gynaecol 21:83-100. 2007
    ..It is therefore important to understand the role of each medium component and to identify possible sources of cellular stress to the embryo that will ultimately affect the function and viability of the conceptus...
  38. ncbi request reprint Oxidative metabolism of pyruvate is required for meiotic maturation of murine oocytes in vivo
    Mark T Johnson
    Department of Genetics, Case School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Biol Reprod 77:2-8. 2007
    ..At earlier stages of oogenesis this metabolic pathway may not be necessary due to metabolic compensation by the granulosa cells...
  39. ncbi request reprint Management of poor responders: can outcomes be improved with a novel gonadotropin-releasing hormone antagonist/letrozole protocol?
    William B Schoolcraft
    Colorado Center for Reproductive Medicine, Englewood, Colorado, USA
    Fertil Steril 89:151-6. 2008
    ..To compare the efficacy of a microdose GnRH agonist flare (ML) with a GnRH antagonist/letrozole (AL) protocol before IVF-ET in poor responders...
  40. ncbi request reprint Exposure of mouse oocytes to 1,2-propanediol during slow freezing alters the proteome
    Mandy G Katz-Jaffe
    Colorado Center for Reproductive Medicine, Englewood, Colorado 80113, USA
    Fertil Steril 89:1441-7. 2008
    ..To examine the effect of exposure to 1,2-propanediol (PrOH) on the mammalian oocyte proteome...
  41. ncbi request reprint Analysis of protein expression (secretome) by human and mouse preimplantation embryos
    Mandy G Katz-Jaffe
    Colorado Center for Reproductive Medicine, Englewood, USA
    Fertil Steril 86:678-85. 2006
    ..Because the majority of biologic functions are carried out by proteins, it is important to study the dynamics of the proteome during embryonic development and its response to both internal and external stimuli...
  42. ncbi request reprint Choosing between day 3 and day 5 embryo transfers
    David K Gardner
    Colorado Center for Reproductive Medicine, Englewood, Colorado 80113
    Clin Obstet Gynecol 49:85-92. 2006
  43. ncbi request reprint Vitrification of mouse pronuclear oocytes with no direct liquid nitrogen contact
    Mark G Larman
    Colorado Centre for Reproductive Medicine, 799 East Hampden Avenue, Suite 520, Englewood, CO 80113, USA
    Reprod Biomed Online 12:66-9. 2006
    ..When such vitrified embryos are warmed, they are capable of subsequent development comparable with non-vitrified embryos...
  44. ncbi request reprint Mitochondrial malate-aspartate shuttle regulates mouse embryo nutrient consumption
    Michelle Lane
    Research Department, Colorado Center for Reproductive Medicine, Englewood, Colorado 80110, USA
    J Biol Chem 280:18361-7. 2005
    ..This study determined that the malate-aspartate shuttle is a key regulator of embryo metabolism and therefore viability and is the first report that mouse zygotes can develop normally to term in the absence of pyruvate...
  45. ncbi request reprint Oestradiol, cyclodextrin-encapsulated 17beta-oestradiol and the oestradiol solubilizer 2-hydroxypropyl-beta-cyclodextrin all impair preimplantation mouse embryo development
    Levent Karagenc
    Bahceci Women Health Care Centre and German Hospital in Istanbul
    Reprod Biomed Online 9:280-6. 2004
    ..Trophectoderm, ICM and blastocyst cell numbers as well as percentage ICM development were reduced significantly, regardless of the stage of development. Therefore, 17beta-oestradiol does compromise embryo development...
  46. ncbi request reprint Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization
    Emre Seli
    Department of Obstetrics and Gynecology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
    Fertil Steril 82:378-83. 2004
    ..To determine whether the extent of ongoing apoptotic cell death measured as the presence of DNA strand breaks in spermatozoa affects embryo development to the blastocyst stage in IVF...
  47. ncbi request reprint Cryosystem assessment by glucose uptake of murine blastocysts
    David L Walker
    Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, Section of Reproductive Endocrinology and Infertility, Charlton Building Desk 3 A, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Reprod Biomed Online 11:601-7. 2005
    ..Glucose uptake appears to be a sensitive, non-invasive method to validate cryopreservation protocols...
  48. ncbi request reprint Ammonium induces aberrant blastocyst differentiation, metabolism, pH regulation, gene expression and subsequently alters fetal development in the mouse
    Michelle Lane
    Research Department, Colorado Center for Reproductive Medicine, Englewood, Colorado 80110, USA
    Biol Reprod 69:1109-17. 2003
    ..It is therefore essential that culture conditions for mammalian embryos are designed to minimize the buildup of ammonium to prevent abnormalities in embryo physiology, genetic regulation, pregnancy, and fetal development...
  49. ncbi request reprint Use of G1.2/G2.2 media for commercial bovine embryo culture: equivalent development and pregnancy rates compared to co-culture
    Michelle Lane
    Research and Development Department, Colorado Center for Reproductive Medicine, 799 East Hampden Avenue, Suite 520, Englewood, CO 80110, USA
    Theriogenology 60:407-19. 2003
    ..2/G2.2 could be used in a commercial OPU transfer program without any loss in the numbers of blastocysts produced or the numbers of pregnancies resulting following transfer from either fresh or cryopreserved blastocysts...
  50. ncbi request reprint Cryo-survival and development of bovine blastocysts are enhanced by culture with recombinant albumin and hyaluronan
    Michelle Lane
    Research Department, Colorado Center for Reproductive Medicine, Englewood, Colorado 80110, USA
    Mol Reprod Dev 64:70-8. 2003
    ..For bovine embryos this has implications for both research and commercial applications where defined reproducible conditions are desirable...
  51. ncbi request reprint Addition of ascorbate during cryopreservation stimulates subsequent embryo development
    Michelle Lane
    Research Department, Colorado Center for Reproductive Medicine, 799 East Hampden Ave, Suite 520, Englewood 80110, USA
    Hum Reprod 17:2686-93. 2002
    ..Therefore, this study investigated the effects of the addition of the antioxidant ascorbate to the cryopreservation solutions on subsequent embryo development...
  52. ncbi request reprint Understanding cellular disruptions during early embryo development that perturb viability and fetal development
    Michelle Lane
    Research Centre for Reproductive Health, Department of Obstetrics and Gynaecology, University of Adelaide, Woodville, Australia
    Reprod Fertil Dev 17:371-8. 2005
    ....
  53. ncbi request reprint Noninvasive methods to assess embryo quality
    Denny Sakkas
    Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut 06520 8063, USA
    Curr Opin Obstet Gynecol 17:283-8. 2005
    ....
  54. ncbi request reprint Proteomic analysis of individual human embryos to identify novel biomarkers of development and viability
    Mandy G Katz-Jaffe
    Colorado Center for Reproductive Medicine, Englewood, Colorado 80113, USA
    Fertil Steril 85:101-7. 2006
    ..To develop a method to analyze the proteome of individual human blastocysts and identify differentially expressed proteins prior to implantation...
  55. ncbi request reprint Calcium-free vitrification reduces cryoprotectant-induced zona pellucida hardening and increases fertilization rates in mouse oocytes
    Mark G Larman
    Colorado Center for Reproductive Medicine, 799 East Hampden Avenue, Suite 520, Englewood, 80113, USA
    Reproduction 131:53-61. 2006
    ....
  56. ncbi request reprint A proteomic analysis of mammalian preimplantation embryonic development
    Mandy G Katz-Jaffe
    Colorado Center for Reproductive Medicine, 799 E Hampden Ave, Suite 520, Englewood, Colorado 80113, USA
    Reproduction 130:899-905. 2005
    ....
  57. ncbi request reprint Metabolism, protein content, and in vitro embryonic development of goat cumulus-oocyte complexes matured with physiological concentrations of glucose and L-lactate
    Jason R Herrick
    Department of Animal Sciences, Program of Comparative Medicine, Purdue University, West Lafayette, IN 47907 2054, USA
    Mol Reprod Dev 73:256-66. 2006
    ..These results indicate that concentrations of glucose and lactate in the medium have cell type-specific effects on metabolism of oocytes and COCs, but do not affect developmental competence within the range of concentrations tested...
  58. ncbi request reprint Metabolic markers of developmental competence for in vitro-matured mouse oocytes
    Kimberly A Preis
    Colorado Center for Reproductive Medicine, 799 East Hampden Avenue, Englewood, CO 80113, USA
    Reproduction 130:475-83. 2005
    ..05). These data indicate that the final hours of maturation may hold a unique marker of oocyte competence, as during this time fertilizable COCs take up more glucose and produce more lactate than those not subsequently fertilized...
  59. ncbi request reprint Granulocyte-macrophage colony-stimulating factor stimulates mouse blastocyst inner cell mass development only when media lack human serum albumin
    Levent Karagenc
    Bahceci Women Health Care Centre and German Hospital, Istanbul, Turkey
    Reprod Biomed Online 10:511-8. 2005
    ..However, when culture conditions are suboptimal and non-physiological, i.e. the absence of protein, GM-CSF stimulates development of both total cell numbers and ICM development of CF1 blastocysts...
  60. pmc Vitrification of human blastocysts using the cryoloop method: successful clinical application and birth of offspring
    Michael L Reed
    Center for Reproductive Medicine of New Mexico, Albuquerque 87106, USA
    J Assist Reprod Genet 19:304-6. 2002
    ..The purpose was to investigate vitrification of human blastocysts using the cryoloop...
  61. pmc Noninvasive metabolic profiling using microfluidics for analysis of single preimplantation embryos
    John Paul Urbanski
    Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Anal Chem 80:6500-7. 2008
    ..As a proof of principle, metabolic activities of single murine embryos were evaluated using this device...
  62. ncbi request reprint Heterogeneity and oxidation status of commercial human albumin preparations in clinical use
    David Bar-Or
    Swedish Medical Center, Trauma Research Laboratory, Englewood, CO 80113, USA
    Crit Care Med 33:1638-41. 2005
    ..We sought to analyze various commercially available albumin preparations for common, posttranslational modifications...
  63. ncbi request reprint Do patient demographics impact physician success rates?
    William B Schoolcraft
    Colorado Center for Reproductive Medicine, Englewood, Colorado 80110, USA
    Fertil Steril 81:951; discussion 952-3. 2004
    ..Variations in individual physician success rates in IVF might not be attributable to patient demographics...