RAMESH K AKKINA

Summary

Affiliation: Colorado State University
Country: USA

Publications

  1. ncbi New generation humanized mice for virus research: comparative aspects and future prospects
    Ramesh Akkina
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Virology 435:14-28. 2013
  2. ncbi Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy
    Joseph S Anderson
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Retrovirology 3:24. 2006
  3. ncbi CXCR4 and CCR5 shRNA transgenic CD34+ cell derived macrophages are functionally normal and resist HIV-1 infection
    Joseph Anderson
    Dept Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Retrovirology 2:53. 2005
  4. ncbi HIV-1 infection and CD4 T cell depletion in the humanized Rag2-/-gamma c-/- (RAG-hu) mouse model
    Bradford K Berges
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Retrovirology 3:76. 2006
  5. ncbi Humanized Rag1-/- γc-/- mice support multilineage hematopoiesis and are susceptible to HIV-1 infection via systemic and vaginal routes
    Ramesh Akkina
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, United States of America
    PLoS ONE 6:e20169. 2011
  6. ncbi siRNAs, ribozymes and RNA decoys in modeling stem cell-based gene therapy for HIV/AIDS
    Ramesh Akkina
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1619, USA
    Anticancer Res 23:1997-2005. 2003
  7. ncbi RNA-based anti-HIV-1 gene therapeutic constructs in SCID-hu mouse model
    Jirong Bai
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Mol Ther 6:770-82. 2002
  8. ncbi TRIM5alpharh expression restricts HIV-1 infection in lentiviral vector-transduced CD34+-cell-derived macrophages
    Joseph Anderson
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, 80523, USA
    Mol Ther 12:687-96. 2005
  9. ncbi Inhibition of HIV-1 by lentiviral vector-transduced siRNAs in T lymphocytes differentiated in SCID-hu mice and CD34+ progenitor cell-derived macrophages
    Akhil Banerjea
    Department of Microbiology, Immunology and Pathology, Colorado State University, 1619 Campus Drive, Fort Collins, Colorado 80523, USA
    Mol Ther 8:62-71. 2003
  10. ncbi Mucosal transmission of R5 and X4 tropic HIV-1 via vaginal and rectal routes in humanized Rag2-/- gammac -/- (RAG-hu) mice
    Bradford K Berges
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Virology 373:342-51. 2008

Research Grants

  1. Humanized Rag2-/-gammac-/-mice for HIV infection and gene therapy
    Ramesh Akkina; Fiscal Year: 2007
  2. SiRNA Gene Therapy for HIV/AIDS
    Ramesh Akkina; Fiscal Year: 2007
  3. Modeling RNA-based HIV gene therapeutics in SCID-hu mice
    Ramesh Akkina; Fiscal Year: 2006
  4. Humanized Rag2-/-gammac-/-mice for HIV infection and gene therapy
    Ramesh Akkina; Fiscal Year: 2010

Collaborators

Detail Information

Publications16

  1. ncbi New generation humanized mice for virus research: comparative aspects and future prospects
    Ramesh Akkina
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Virology 435:14-28. 2013
    ....
  2. ncbi Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy
    Joseph S Anderson
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Retrovirology 3:24. 2006
    ....
  3. ncbi CXCR4 and CCR5 shRNA transgenic CD34+ cell derived macrophages are functionally normal and resist HIV-1 infection
    Joseph Anderson
    Dept Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Retrovirology 2:53. 2005
    ..To extend these results to a stem cell gene therapy setting, here we show transduction of primary CD34+ hematopoietic progenitor cells to derive normal end stage cells that are resistant to HIV-1 infection...
  4. ncbi HIV-1 infection and CD4 T cell depletion in the humanized Rag2-/-gamma c-/- (RAG-hu) mouse model
    Bradford K Berges
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Retrovirology 3:76. 2006
    ..Thus, this model shows considerable promise to study long-term in vivo HIV infection and pathogenesis...
  5. ncbi Humanized Rag1-/- γc-/- mice support multilineage hematopoiesis and are susceptible to HIV-1 infection via systemic and vaginal routes
    Ramesh Akkina
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, United States of America
    PLoS ONE 6:e20169. 2011
    ..Thus these mice can be further exploited for studying human pathogens that infect the human hematopoietic system in an in vivo setting...
  6. ncbi siRNAs, ribozymes and RNA decoys in modeling stem cell-based gene therapy for HIV/AIDS
    Ramesh Akkina
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1619, USA
    Anticancer Res 23:1997-2005. 2003
    ..When challenged in vitro with HIV-1, they showed significant anti-viral resistance. These proof-of-concept studies demonstrated the utility of RNA-based anti-HIV constructs for gene therapy...
  7. ncbi RNA-based anti-HIV-1 gene therapeutic constructs in SCID-hu mouse model
    Jirong Bai
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Mol Ther 6:770-82. 2002
    ..These results highlight the utility of RNA-based anti-HIV-1 gene therapeutic approaches and their preclinical testing in a surrogate animal model harboring human tissue...
  8. ncbi TRIM5alpharh expression restricts HIV-1 infection in lentiviral vector-transduced CD34+-cell-derived macrophages
    Joseph Anderson
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, 80523, USA
    Mol Ther 12:687-96. 2005
    ..1. These results demonstrate that the species-specific restriction factor TRIM5alpha(rh) is effective in conferring HIV-1 resistance in a stem cell setting, thus paving the way for its application in AIDS gene therapy...
  9. ncbi Inhibition of HIV-1 by lentiviral vector-transduced siRNAs in T lymphocytes differentiated in SCID-hu mice and CD34+ progenitor cell-derived macrophages
    Akhil Banerjea
    Department of Microbiology, Immunology and Pathology, Colorado State University, 1619 Campus Drive, Fort Collins, Colorado 80523, USA
    Mol Ther 8:62-71. 2003
    ..These experiments demonstrate the utility of siRNAs delivered into hematopoietic stem cells via lentiviral vectors for future in vivo applications...
  10. ncbi Mucosal transmission of R5 and X4 tropic HIV-1 via vaginal and rectal routes in humanized Rag2-/- gammac -/- (RAG-hu) mice
    Bradford K Berges
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Virology 373:342-51. 2008
    ....
  11. ncbi Humanized Rag2(-/-)gammac(-/-) (RAG-hu) mice can sustain long-term chronic HIV-1 infection lasting more than a year
    Bradford K Berges
    Department of Microbiology, Immunology, and Pathology, Colorado State University, 329 Pathology, Fort Collins, CO 80523, USA
    Virology 397:100-3. 2010
    ..These studies provide a chronic HIV-1 infection humanized mouse model that can be used to dissect viral latency, long-term drug evaluation and immune-based therapies...
  12. ncbi Dengue virus infection and immune response in humanized RAG2(-/-)gamma(c)(-/-) (RAG-hu) mice
    Jes G Kuruvilla
    Department of Microbiology, Immunology and Pathology, 1619 Campus Delivery, Colorado State University, Fort Collins, CO 80523 1619, USA
    Virology 369:143-52. 2007
    ..These results demonstrate for the first time that humanized mice are capable of dengue viral primary human immune responses thus paving the way for new dengue immunopathogenesis and vaccine studies...
  13. ncbi Human immunodeficiency virus type 1 restriction by human-rhesus chimeric tripartite motif 5alpha (TRIM 5alpha) in CD34(+) cell-derived macrophages in vitro and in T cells in vivo in severe combined immunodeficient (SCID-hu) mice transplanted with human fe
    Joseph Anderson
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Hum Gene Ther 19:217-28. 2008
    ..These results demonstrate the efficacy of TRIM5alpha-HRH in a stem cell setting and its further advancement for use in gene therapy applications...
  14. ncbi Safety and efficacy of a lentiviral vector containing three anti-HIV genes--CCR5 ribozyme, tat-rev siRNA, and TAR decoy--in SCID-hu mouse-derived T cells
    Joseph Anderson
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort, Collins, Colorado 80523, USA
    Mol Ther 15:1182-8. 2007
    ..These important attributes of this combinatorial vector show its promise as an excellent candidate for use in human clinical trials...
  15. ncbi Potent suppression of HIV type 1 infection by a short hairpin anti-CXCR4 siRNA
    Joseph Anderson
    Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA
    AIDS Res Hum Retroviruses 19:699-706. 2003
    ..Delivery of this siRNA into hematopoietic stem cells via lentiviral vectors may have potential gene therapeutic applications...
  16. ncbi Bispecific short hairpin siRNA constructs targeted to CD4, CXCR4, and CCR5 confer HIV-1 resistance
    Joseph Anderson
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Oligonucleotides 13:303-12. 2003
    ....

Research Grants15

  1. Humanized Rag2-/-gammac-/-mice for HIV infection and gene therapy
    Ramesh Akkina; Fiscal Year: 2007
    ....
  2. SiRNA Gene Therapy for HIV/AIDS
    Ramesh Akkina; Fiscal Year: 2007
    ..4. Transduce siRNA constructs into the newly described CD34+/KDR+ primitive hematopoietic progenitor cells and derive macrophages in vitro and thymocytes in vivo, and evaluate their HIV-1 resistance. ..
  3. Modeling RNA-based HIV gene therapeutics in SCID-hu mice
    Ramesh Akkina; Fiscal Year: 2006
    ..4) Determine the engraftment and thymopoietic potential of lentivirally transduced primitive hematopoietic precursor cells i.e., a) CD34+ and KDR+ cells, b) SP cells in the SCID-hu mouse thymic microenvironment. ..
  4. Humanized Rag2-/-gammac-/-mice for HIV infection and gene therapy
    Ramesh Akkina; Fiscal Year: 2010
    ....