Karel Svoboda

Summary

Affiliation: Cold Spring Harbor Laboratory
Country: USA

Publications

  1. pmc The spread of Ras activity triggered by activation of a single dendritic spine
    Christopher D Harvey
    Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
    Science 321:136-40. 2008
  2. ncbi request reprint Principles of two-photon excitation microscopy and its applications to neuroscience
    Karel Svoboda
    HHMI, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Neuron 50:823-39. 2006
  3. pmc Rapid functional maturation of nascent dendritic spines
    Karen Zito
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Neuron 61:247-58. 2009
  4. ncbi request reprint Geometric and functional organization of cortical circuits
    Gordon M G Shepherd
    Howard Hughes Medical Institute, Cold Spring Harbor, New York 11724, USA
    Nat Neurosci 8:782-90. 2005
  5. ncbi request reprint Activity-dependent plasticity of the NMDA-receptor fractional Ca2+ current
    Aleksander Sobczyk
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Neuron 53:17-24. 2007
  6. ncbi request reprint Cell type-specific structural plasticity of axonal branches and boutons in the adult neocortex
    Vincenzo de Paola
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Neuron 49:861-75. 2006
  7. pmc The functional asymmetry of auditory cortex is reflected in the organization of local cortical circuits
    HYSELL V OVIEDO
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA
    Nat Neurosci 13:1413-20. 2010
  8. pmc Circuit and plasticity defects in the developing somatosensory cortex of FMR1 knock-out mice
    Ingrid Bureau
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Neurosci 28:5178-88. 2008
  9. pmc Structural plasticity underlies experience-dependent functional plasticity of cortical circuits
    Linda Wilbrecht
    Howard Hughes Medical Institute HHMI, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Neurosci 30:4927-32. 2010
  10. ncbi request reprint Experience-dependent and cell-type-specific spine growth in the neocortex
    Anthony Holtmaat
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Nature 441:979-83. 2006

Research Grants

  1. Optical Studies of Single Hippocampal Synapses
    Karel Svoboda; Fiscal Year: 2006
  2. Optical Studies of Single Hippocampal Synapses
    Karel Svoboda; Fiscal Year: 2007
  3. DENDRITIC FUNCTION IN NEOCORTEX IN VIVO
    Karel Svoboda; Fiscal Year: 2007
  4. Molecules for inducibly modulating synaptic function
    Karel Svoboda; Fiscal Year: 2007
  5. Open source software for laser scanning microscopy
    Karel Svoboda; Fiscal Year: 2007
  6. Optical Studies of Single Hippocampal Synapses
    Karel Svoboda; Fiscal Year: 2003
  7. DENDRITIC FUNCTION IN NEOCORTEX IN VIVO
    Karel Svoboda; Fiscal Year: 2003

Detail Information

Publications52

  1. pmc The spread of Ras activity triggered by activation of a single dendritic spine
    Christopher D Harvey
    Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
    Science 321:136-40. 2008
    ..The spread of Ras-dependent signaling was necessary for the local regulation of the threshold for LTP induction. Thus, Ca2+-dependent synaptic signals can spread to couple multiple synapses on short stretches of dendrite...
  2. ncbi request reprint Principles of two-photon excitation microscopy and its applications to neuroscience
    Karel Svoboda
    HHMI, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Neuron 50:823-39. 2006
    ..Here we review the principles of 2PE microscopy, highlight recent applications, discuss its limitations, and point to areas for future research and development...
  3. pmc Rapid functional maturation of nascent dendritic spines
    Karen Zito
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Neuron 61:247-58. 2009
    ..New spines reconstructed using electron microscopy made synapses. Our data support a model in which outgrowth and enlargement of nascent spines is tightly coupled to formation and maturation of glutamatergic synapses...
  4. ncbi request reprint Geometric and functional organization of cortical circuits
    Gordon M G Shepherd
    Howard Hughes Medical Institute, Cold Spring Harbor, New York 11724, USA
    Nat Neurosci 8:782-90. 2005
    ..A large plasticity potential appears to be incorporated into these circuits, allowing for functional 'tuning' with fixed axonal and dendritic arbor geometry...
  5. ncbi request reprint Activity-dependent plasticity of the NMDA-receptor fractional Ca2+ current
    Aleksander Sobczyk
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Neuron 53:17-24. 2007
    ..Receptors in single spines depressed rapidly in an all-or-none manner. These adaptive changes in NMDA-R function likely play a critical role in metaplasticity and in stabilizing activity levels in neuronal networks with Hebbian synapses...
  6. ncbi request reprint Cell type-specific structural plasticity of axonal branches and boutons in the adult neocortex
    Vincenzo de Paola
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Neuron 49:861-75. 2006
    ..Retrospective electron microscopy revealed that new boutons make synapses. Our data suggest that structural plasticity of axonal branches and boutons contributes to the remodeling of specific functional circuits...
  7. pmc The functional asymmetry of auditory cortex is reflected in the organization of local cortical circuits
    HYSELL V OVIEDO
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA
    Nat Neurosci 13:1413-20. 2010
    ..Our results are consistent with the hypothesis that auditory cortical microcircuitry is specialized to the one-dimensional representation of frequency in the auditory cortex...
  8. pmc Circuit and plasticity defects in the developing somatosensory cortex of FMR1 knock-out mice
    Ingrid Bureau
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Neurosci 28:5178-88. 2008
    ..By 3 weeks, the strength of the L4-->L3 projection was similar to that of wild type. Our data indicate that Fmr1 shapes sensory cortical circuits during a developmental critical period...
  9. pmc Structural plasticity underlies experience-dependent functional plasticity of cortical circuits
    Linda Wilbrecht
    Howard Hughes Medical Institute HHMI, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Neurosci 30:4927-32. 2010
    ..Our data provide a causal link between new spine synapses and plasticity of adult cortical circuits and suggest that alphaCaMKII autophosphorylation plays a role in the stabilization but not formation of new spines...
  10. ncbi request reprint Experience-dependent and cell-type-specific spine growth in the neocortex
    Anthony Holtmaat
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Nature 441:979-83. 2006
    ..Our data indicate that novel sensory experience drives the stabilization of new spines on subclasses of cortical neurons. These synaptic changes probably underlie experience-dependent remodelling of specific neocortical circuits...
  11. ncbi request reprint Transient and persistent dendritic spines in the neocortex in vivo
    Anthony J G D Holtmaat
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Neuron 45:279-91. 2005
    ..In 6-month-old mice, spines turn over more slowly in visual compared to somatosensory cortex, possibly reflecting differences in the capacity for experience-dependent plasticity in these brain regions...
  12. ncbi request reprint Dendrodendritic synaptic signals in olfactory bulb granule cells: local spine boost and global low-threshold spike
    Veronica Egger
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Neurosci 25:3521-30. 2005
    ..The coincidence of local input and LTS in the spine resulted in summation of local and global Ca2+ signals, a dendritic computation that could endow granule cells with subthreshold associative plasticity...
  13. doi request reprint Long-range neuronal circuits underlying the interaction between sensory and motor cortex
    Tianyi Mao
    Janelia Farm Research Campus, HHMI, 19700 Helix Drive, Ashburn, VA 20147, USA
    Neuron 72:111-23. 2011
    ..The cortico-cortical neurons in superficial layers of motor cortex thus couple motor and sensory signals and might mediate sensorimotor integration and motor learning...
  14. ncbi request reprint Nonlinear [Ca2+] signaling in dendrites and spines caused by activity-dependent depression of Ca2+ extrusion
    Volker Scheuss
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Neurosci 26:8183-94. 2006
    ..Depression of Ca2+ extrusion produces Ca2+ concentration dynamics that depend on the history of neuronal activity and therefore likely modulates the induction of synaptic plasticity...
  15. ncbi request reprint Monitoring neural activity and [Ca2+] with genetically encoded Ca2+ indicators
    Thomas A Pologruto
    Howard Hughes Medical Institute Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Neurosci 24:9572-9. 2004
    ..We compared GECI fluorescence saturation with CaM Ca2+-dependent structural transitions. Our data suggest that GCaMP and Camgaroo2 report CaM structural transitions in the presence and absence of CaM-binding peptide, respectively...
  16. ncbi request reprint Induction of spine growth and synapse formation by regulation of the spine actin cytoskeleton
    Karen Zito
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Neuron 44:321-34. 2004
    ..Synaptic strengths were reduced to compensate for extra synapses, keeping total synaptic input per neuron constant. Our data support a model in which synapse formation is promoted by actin-powered motility...
  17. pmc Interdigitated paralemniscal and lemniscal pathways in the mouse barrel cortex
    Ingrid Bureau
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America
    PLoS Biol 4:e382. 2006
    ..L2 neurons received input from lemniscal L3 cells and paralemniscal L5A cells spread over multiple columns. Our data indicate that lemniscal and paralemniscal information is segregated into interdigitated cortical layers...
  18. pmc Rapid redistribution of synaptic PSD-95 in the neocortex in vivo
    Noah W Gray
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA
    PLoS Biol 4:e370. 2006
    ..Our data suggest that individual PSDs compete for PSD-95 and that the kinetic interactions between PSD molecules and PSDs are tuned to regulate PSD size...
  19. pmc Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window
    Anthony Holtmaat
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, NY, USA
    Nat Protoc 4:1128-44. 2009
    ..The entire dendritic and axonal arbor of individual neurons can be reconstructed...
  20. ncbi request reprint NMDA receptor subunit-dependent [Ca2+] signaling in individual hippocampal dendritic spines
    Aleksander Sobczyk
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Neurosci 25:6037-46. 2005
    ..Our data suggest that individual spines can regulate the subunit composition of their NMDA-Rs and the effective fractional Ca2+ current through these receptors...
  21. pmc Locally dynamic synaptic learning rules in pyramidal neuron dendrites
    Christopher D Harvey
    Janelia Farm Research Campus, HHMI, Ashburn, Virginia 20147, USA
    Nature 450:1195-200. 2007
    ..These local interactions between neighbouring synapses support clustered plasticity models of memory storage and could allow for the binding of behaviourally linked information on the same dendritic branch...
  22. doi request reprint Learning-related fine-scale specificity imaged in motor cortex circuits of behaving mice
    Takaki Komiyama
    Janelia Farm Research Campus, HHMI, Ashburn, Virginia 20147, USA
    Nature 464:1182-6. 2010
    ..Our findings reveal a fine-scale and dynamic organization of the frontal cortex that probably underlies flexible behaviour...
  23. ncbi request reprint Laminar and columnar organization of ascending excitatory projections to layer 2/3 pyramidal neurons in rat barrel cortex
    Gordon M G Shepherd
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Neurosci 25:5670-9. 2005
    ....
  24. ncbi request reprint Precise development of functional and anatomical columns in the neocortex
    Ingrid Bureau
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Neuron 42:789-801. 2004
    ..Consistent with this, synaptic currents measured in layer 2/3 neurons at PND 8, just after these neurons ceased to migrate, revealed already spatially well-tuned receptive fields...
  25. ncbi request reprint The life cycle of Ca(2+) ions in dendritic spines
    Bernardo L Sabatini
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Neuron 33:439-52. 2002
    ..Furthermore, the kinetics of Ca(2+) sources governs the time course of [Ca(2+)] signals and may explain the selective activation of long-term synaptic potentiation (LTP) and long-term depression (LTD) by NMDA-R-mediated synaptic Ca(2+)...
  26. ncbi request reprint Subcellular domain-restricted GABAergic innervation in primary visual cortex in the absence of sensory and thalamic inputs
    Graziella Di Cristo
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Nat Neurosci 7:1184-6. 2004
    ..Therefore, subcellular synapse targeting is independent of thalamic input and probably involves molecular labels and experience-independent forms of activity...
  27. pmc Characterization and subcellular targeting of GCaMP-type genetically-encoded calcium indicators
    Tianyi Mao
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America
    PLoS ONE 3:e1796. 2008
    ..Our results show that GCaMP2 is improved from the previous versions of GCaMP and may be suited to detect bursts of high-frequency action potentials and synaptic currents in vivo...
  28. pmc Diverse modes of axon elaboration in the developing neocortex
    Carlos Portera-Cailliau
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA
    PLoS Biol 3:e272. 2005
    ..Given the identical layer 1 territory upon which CR and TC axons grow, the differences in their structure and dynamics likely reflect distinct intrinsic growth programs for axons of long projection neurons versus local interneurons...
  29. ncbi request reprint Mechanisms of lateral inhibition in the olfactory bulb: efficiency and modulation of spike-evoked calcium influx into granule cells
    Veronica Egger
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Neurosci 23:7551-8. 2003
    ..The implication of a transiently inactivating calcium channel in synaptic release from granule cells suggests novel mechanisms for the regulation of lateral inhibition in the olfactory bulb...
  30. pmc Subliminal messages in hippocampal pyramidal cells
    Thomas G Oertner
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Rd, Cold Spring Harbor, NY 11724, USA
    J Physiol 543:397. 2002
  31. ncbi request reprint Plasticity of calcium channels in dendritic spines
    Ryohei Yasuda
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road Cold Spring Harbor, New York 11724, USA
    Nat Neurosci 6:948-55. 2003
    ..Given that L-type VSCCs do not contribute measurably to Ca2+ influx in spines, they must activate downstream effectors either directly through voltage-dependent conformational changes or via [Ca2+] microdomains...
  32. pmc A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale
    Jason W Bohland
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA
    PLoS Comput Biol 5:e1000334. 2009
    ..We estimate that the mouse connectivity project could be completed within five years with a comparatively modest budget...
  33. ncbi request reprint Stereotyped odor-evoked activity in the mushroom body of Drosophila revealed by green fluorescent protein-based Ca2+ imaging
    Yalin Wang
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Neurosci 24:6507-14. 2004
    ..These results indicate that odor-evoked activity can have remarkable spatial specificity in the MB...
  34. ncbi request reprint Facilitation at single synapses probed with optical quantal analysis
    Thomas G Oertner
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Neurosci 5:657-64. 2002
    ....
  35. ncbi request reprint Long-term in vivo imaging of experience-dependent synaptic plasticity in adult cortex
    Joshua T Trachtenberg
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Nature 420:788-94. 2002
    ..Our measurements suggest that sensory experience drives the formation and elimination of synapses and that these changes might underlie adaptive remodelling of neural circuits...
  36. doi request reprint Flow of cortical activity underlying a tactile decision in mice
    Zengcai V Guo
    Janelia Farm Research Campus, HHMI, Ashburn, VA 20147, USA
    Neuron 81:179-94. 2014
    ..These results suggest serial information flow from sensory to motor areas during perceptual decision making. ..
  37. ncbi request reprint The number of glutamate receptors opened by synaptic stimulation in single hippocampal spines
    Esther A Nimchinsky
    Howard Hughes Medical Institute, The Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Neurosci 24:2054-64. 2004
    ..Our measurements further demonstrate that optical recordings can be used to study single receptors in intact systems...
  38. ncbi request reprint Experience-dependent changes in basal dendritic branching of layer 2/3 pyramidal neurons during a critical period for developmental plasticity in rat barrel cortex
    Miguel Maravall
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Cereb Cortex 14:655-64. 2004
    ..Deprivation initiated at PND 15 had no effect on basal branching measured at PND 20. Thus the spatial organization of secondary dendritic branching is experience-dependent and shares a critical period with receptive field plasticity...
  39. ncbi request reprint Activity-dependent synaptogenesis in the adult Mammalian cortex
    Karen Zito
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, NY 11724, USA
    Neuron 35:1015-7. 2002
    ..Together with progress imaging synapses in vivo, the stage appears set for advances in understanding the dynamics and mechanisms of experience-dependent synaptogenesis...
  40. ncbi request reprint Imaging calcium concentration dynamics in small neuronal compartments
    Ryohei Yasuda
    Howard Hughes Medical Institute, The Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Sci STKE 2004:pl5. 2004
    ..Here, we describe techniques that are optimized for 2-photon imaging of [Ca2+] dynamics in small compartments such as dendrites and dendritic spines...
  41. ncbi request reprint Development of intrinsic properties and excitability of layer 2/3 pyramidal neurons during a critical period for sensory maps in rat barrel cortex
    Miguel Maravall
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Neurophysiol 92:144-56. 2004
    ..Other measures of excitability, including I-f curves and passive membrane properties, were affected by development but unaffected by whisker deprivation...
  42. ncbi request reprint Structure and function of dendritic spines
    Esther A Nimchinsky
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Annu Rev Physiol 64:313-53. 2002
    ..In particular, recent work has highlighted that spines are highly specialized compartments for rapid large-amplitude Ca(2+) signals underlying the induction of synaptic plasticity...
  43. pmc ScanImage: flexible software for operating laser scanning microscopes
    Thomas A Pologruto
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Biomed Eng Online 2:13. 2003
    ..A major impediment to custom design is the difficulty of building custom data acquisition hardware and writing the complex software required to run the laser scanning microscope...
  44. ncbi request reprint Circuit analysis of experience-dependent plasticity in the developing rat barrel cortex
    Gordon M G Shepherd
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Neuron 38:277-89. 2003
    ..These results reveal competitive interactions between barrel and septal circuits in the establishment of precise intracortical circuits...
  45. ncbi request reprint Experience strengthening transmission by driving AMPA receptors into synapses
    Takuya Takahashi
    Jones Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Science 299:1585-8. 2003
    ..In general, synaptic incorporation of AMPA-Rs in vivo conforms to rules identified in vitro and contributes to plasticity driven by natural stimuli in the mammalian brain...
  46. ncbi request reprint Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging
    Ryohei Yasuda
    Howard Hughes Medical Institute
    Nat Neurosci 9:283-91. 2006
    ..The Ras pathway therefore functions as a supersensitive threshold detector for neural activity and Ca(2+) concentration...
  47. ncbi request reprint Do spines and dendrites distribute dye evenly?
    Karel Svoboda
    Trends Neurosci 27:445-6. 2004
  48. ncbi request reprint Channelrhodopsin-2-assisted circuit mapping of long-range callosal projections
    Leopoldo Petreanu
    Howard Hughes Medical Institute, Janelia Farm Research Campus, 19700 Helix Drive, Ashburn, Virginia 20147, USA
    Nat Neurosci 10:663-8. 2007
    ..In both hemispheres the L2/3-to-L5 projection is stronger than the L2/3-to-L2/3 projection. Our results suggest that laminar specificity may be identical for local and long-range cortical projections...
  49. ncbi request reprint Novel approaches to monitor and manipulate single neurons in vivo
    Michael Brecht
    Department of Neuroscience, Erasmus Medical Center, University Medical Center Rotterdam, 3015 DR Rotterdam, The Netherlands
    J Neurosci 24:9223-7. 2004
  50. ncbi request reprint Rapid and reversible chemical inactivation of synaptic transmission in genetically targeted neurons
    Alla Y Karpova
    Howard Hughes Medical Institute, USA
    Neuron 48:727-35. 2005
    ..MISTs allow for specific, inducible, and reversible lesions in neuronal circuits and may provide treatment of disorders associated with neuronal hyperactivity...
  51. ncbi request reprint An image analysis algorithm for dendritic spines
    Ingrid Y Y Koh
    Department of Applied Mathematics and Statistics, State University of New York at Stony Brook, Stony Brook, NY 11794 3600, USA
    Neural Comput 14:1283-310. 2002
    ..Automated analysis of dendritic spine morphology will enable objective analysis of large morphological data sets. The approaches presented here are generalizable to other aspects of neuronal morphology...
  52. pmc Sparse optical microstimulation in barrel cortex drives learned behaviour in freely moving mice
    Daniel Huber
    Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia 20147, USA
    Nature 451:61-4. 2008
    ..Our results show that perceptual decisions and learning can be driven by extremely brief epochs of cortical activity in a sparse subset of supragranular cortical pyramidal neurons...

Research Grants25

  1. Optical Studies of Single Hippocampal Synapses
    Karel Svoboda; Fiscal Year: 2006
    ..We hope to end up with a core description of the function and plasticity of single synapses. ..
  2. Optical Studies of Single Hippocampal Synapses
    Karel Svoboda; Fiscal Year: 2007
    ..We hope to end up with a core description of the function and plasticity of single synapses. ..
  3. DENDRITIC FUNCTION IN NEOCORTEX IN VIVO
    Karel Svoboda; Fiscal Year: 2007
    ..abstract_text> ..
  4. Molecules for inducibly modulating synaptic function
    Karel Svoboda; Fiscal Year: 2007
    ..3.) Well-characterized systems will be introduced into subpopulations of neurons in mice using viruses and transgenic approaches. Systems will be tested in vivo using intrinsic signal imaging and electrophysiological techniques. ..
  5. Open source software for laser scanning microscopy
    Karel Svoboda; Fiscal Year: 2007
    ..We believe that Scanlmage will facilitate exciting biological discovery in numerous laboratories. ..
  6. Optical Studies of Single Hippocampal Synapses
    Karel Svoboda; Fiscal Year: 2003
    ..We hope to end up with a core description of the function and plasticity of single synapses. ..
  7. DENDRITIC FUNCTION IN NEOCORTEX IN VIVO
    Karel Svoboda; Fiscal Year: 2003
    ..These studies will be helped by improvements to existing instrumentation for TPLSM-based physiology in vivo. ..