Thomas A Pologruto
Affiliation: Cold Spring Harbor Laboratory
- ScanImage: flexible software for operating laser scanning microscopesThomas A Pologruto
Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
Biomed Eng Online 2:13. 2003..A major impediment to custom design is the difficulty of building custom data acquisition hardware and writing the complex software required to run the laser scanning microscope...
- Monitoring neural activity and [Ca2+] with genetically encoded Ca2+ indicatorsThomas A Pologruto
Howard Hughes Medical Institute Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
J Neurosci 24:9572-9. 2004..We compared GECI fluorescence saturation with CaM Ca2+-dependent structural transitions. Our data suggest that GCaMP and Camgaroo2 report CaM structural transitions in the presence and absence of CaM-binding peptide, respectively...
- Stereotyped odor-evoked activity in the mushroom body of Drosophila revealed by green fluorescent protein-based Ca2+ imagingYalin Wang
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
J Neurosci 24:6507-14. 2004..These results indicate that odor-evoked activity can have remarkable spatial specificity in the MB...
- Imaging calcium concentration dynamics in small neuronal compartmentsRyohei Yasuda
Howard Hughes Medical Institute, The Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
Sci STKE 2004:pl5. 2004..Here, we describe techniques that are optimized for 2-photon imaging of [Ca2+] dynamics in small compartments such as dendrites and dendritic spines...