Research Topics
Genomes and GenesSpecies | James HicksSummaryAffiliation: Cold Spring Harbor Laboratory Country: USA Publications
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Detail Information
Publications
Future medical applications of single-cell sequencing in cancerNicholas Navin
Department of Genetics, MD Anderson Cancer Center, Houston, TX 77030, USA
Genome Med 3:31. 2011..In this review we discuss the challenges and technical aspects of single-cell sequencing, with a strong focus on genomic copy number, and discuss how this information can be used to diagnose and treat cancer patients...
Comparison of the Agilent, ROMA/NimbleGen and Illumina platforms for classification of copy number alterations in human breast tumorsL O Baumbusch
Department of Genetics, Institute for Cancer Research, Norwegian Radium Hospital, Rikshospitalet University Hospital, 0310 Oslo, Norway
BMC Genomics 9:379. 2008..Various platforms, brands and underlying technologies are available, facing the user with many choices regarding platform sensitivity and number, localization, and density distribution of probes...
Novel patterns of genome rearrangement and their association with survival in breast cancerJames Hicks
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
Genome Res 16:1465-79. 2006....
Tumour evolution inferred by single-cell sequencingNicholas Navin
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
Nature 472:90-4. 2011..In contrast to gradual models of tumour progression, our data indicate that tumours grow by punctuated clonal expansions with few persistent intermediates...
Strong association of de novo copy number mutations with autismJonathan Sebat
Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
Science 316:445-9. 2007..Affected genomic regions were highly heterogeneous and included mutations of single genes. These findings establish de novo germline mutation as a more significant risk factor for ASD than previously recognized...
Tracing the tumor lineageNicholas E Navin
Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
Mol Oncol 4:267-83. 2010..Finally, we discuss how recent technical advances in single cell genomics will herald a new era in understanding the fundamental basis of tumor heterogeneity and progression...
Genome-wide copy number analysis of single cellsTimour Baslan
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA
Nat Protoc 7:1024-41. 2012..All together, the protocol takes ∼3 d from flow cytometry to sequence-ready DNA libraries...
Inferring tumor progression from genomic heterogeneityNicholas Navin
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
Genome Res 20:68-80. 2010..By comparing multiple subpopulations from different anatomic locations, we have inferred pathways of cancer progression and the organization of tumor growth...
A cluster of cooperating tumor-suppressor gene candidates in chromosomal deletionsWen Xue
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
Proc Natl Acad Sci U S A 109:8212-7. 2012..Our data imply that large cancer-associated deletions can produce phenotypes distinct from those arising through loss of a single TSG, and as such should be considered and studied as distinct mutational events...
Functional identification of tumor-suppressor genes through an in vivo RNA interference screen in a mouse lymphoma modelAnka Bric
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
Cancer Cell 16:324-35. 2009..Our results emphasize the utility of in vivo RNAi screens, identify and validate a diverse set of tumor suppressors, and have therapeutic implications...
Large-scale copy number polymorphism in the human genomeJonathan Sebat
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
Science 305:525-8. 2004..We observed copy number variation of 70 different genes within CNP intervals, including genes involved in neurological function, regulation of cell growth, regulation of metabolism, and several genes known to be associated with disease...
DDX5 regulates DNA replication and is required for cell proliferation in a subset of breast cancer cellsAnthony Mazurek
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
Cancer Discov 2:812-25. 2012....
An oncogenomics-based in vivo RNAi screen identifies tumor suppressors in liver cancerLars Zender
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
Cell 135:852-64. 2008..Our results establish the feasibility of in vivo RNAi screens and illustrate how combining cancer genomics, RNA interference, and mosaic mouse models can facilitate the functional annotation of the cancer genome...
Identification of alterations in DNA copy number in host stromal cells during tumor progressionRobert J Pelham
Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA
Proc Natl Acad Sci U S A 103:19848-53. 2006..We show that numerous amplifications and deletions are found within the host stromal microenvironment, suggesting that alterations in host DNA copy number can occur and may play a significant role in modifying tumor-stromal interactions...
