Gregory J Hannon

Summary

Affiliation: Cold Spring Harbor Laboratory
Country: USA

Publications

  1. ncbi request reprint RNA interference
    Gregory J Hannon
    Cold Spring Harbour Laboratory, New York 11724, USA
    Nature 418:244-51. 2002
  2. ncbi request reprint RNA interference: the new somatic cell genetics?
    Patrick J Paddison
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Cancer Cell 2:17-23. 2002
  3. pmc Stable suppression of gene expression by RNAi in mammalian cells
    Patrick J Paddison
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 99:1443-8. 2002
  4. ncbi request reprint Synthetic shRNAs as potent RNAi triggers
    Despina Siolas
    Nat Biotechnol 23:227-31. 2005
  5. ncbi request reprint Processing of primary microRNAs by the Microprocessor complex
    Ahmet M Denli
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 432:231-5. 2004
  6. pmc Pseudogene-derived small interfering RNAs regulate gene expression in mouse oocytes
    Oliver H Tam
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 453:534-8. 2008
  7. ncbi request reprint Purified Argonaute2 and an siRNA form recombinant human RISC
    Fabiola V Rivas
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, New York 11724, USA
    Nat Struct Mol Biol 12:340-9. 2005
  8. ncbi request reprint microRNAs join the p53 network--another piece in the tumour-suppression puzzle
    Lin He
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Rev Cancer 7:819-22. 2007
  9. pmc Critical roles for Dicer in the female germline
    Elizabeth P Murchison
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, Cold Spring Harbor, New York 11724, USA
    Genes Dev 21:682-93. 2007
  10. pmc An endogenous small interfering RNA pathway in Drosophila
    Benjamin Czech
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 453:798-802. 2008

Detail Information

Publications71

  1. ncbi request reprint RNA interference
    Gregory J Hannon
    Cold Spring Harbour Laboratory, New York 11724, USA
    Nature 418:244-51. 2002
    ..RNAi has been cultivated as a means to manipulate gene expression experimentally and to probe gene function on a whole-genome scale...
  2. ncbi request reprint RNA interference: the new somatic cell genetics?
    Patrick J Paddison
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Cancer Cell 2:17-23. 2002
    ....
  3. pmc Stable suppression of gene expression by RNAi in mammalian cells
    Patrick J Paddison
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 99:1443-8. 2002
    ....
  4. ncbi request reprint Synthetic shRNAs as potent RNAi triggers
    Despina Siolas
    Nat Biotechnol 23:227-31. 2005
    ..Maximal inhibition of target genes was achieved at lower concentrations and silencing at 24 h was often greater. These studies provide the basis for an improved approach to triggering experimental silencing via the RNAi pathway...
  5. ncbi request reprint Processing of primary microRNAs by the Microprocessor complex
    Ahmet M Denli
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 432:231-5. 2004
    ..Considered together, these results indicate a role for Pasha in miRNA maturation and miRNA-mediated gene regulation...
  6. pmc Pseudogene-derived small interfering RNAs regulate gene expression in mouse oocytes
    Oliver H Tam
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 453:534-8. 2008
    ..Our findings indicate a function for pseudogenes in regulating gene expression by means of the RNA interference pathway and may, in part, explain the evolutionary pressure to conserve argonaute-mediated catalysis in mammals...
  7. ncbi request reprint Purified Argonaute2 and an siRNA form recombinant human RISC
    Fabiola V Rivas
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, New York 11724, USA
    Nat Struct Mol Biol 12:340-9. 2005
    ..These studies demonstrate that Argonaute proteins catalyze mRNA cleavage within RISC and provide a source of recombinant enzyme for detailed biochemical studies of the RNAi effector complex...
  8. ncbi request reprint microRNAs join the p53 network--another piece in the tumour-suppression puzzle
    Lin He
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Rev Cancer 7:819-22. 2007
    ..These data reinforce the growing awareness that non-coding RNAs are key players in tumour development by placing miRNAs in a central role in a well-known tumour-suppressor network...
  9. pmc Critical roles for Dicer in the female germline
    Elizabeth P Murchison
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, Cold Spring Harbor, New York 11724, USA
    Genes Dev 21:682-93. 2007
    ..Our studies identify Dicer as central to a regulatory network that controls oocyte gene expression programs and that promotes genomic integrity in a cell type notoriously susceptible to aneuploidy...
  10. pmc An endogenous small interfering RNA pathway in Drosophila
    Benjamin Czech
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 453:798-802. 2008
    ..These observations expand the repertoire of small RNAs in Drosophila, adding a class that blurs distinctions based on known biogenesis mechanisms and functional roles...
  11. pmc High definition profiling of mammalian DNA methylation by array capture and single molecule bisulfite sequencing
    Emily Hodges
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Genome Res 19:1593-605. 2009
    ..Methylation, along with specific histone marks, was enriched in exonic regions, suggesting that chromatin states can foreshadow the content of mature mRNAs...
  12. ncbi request reprint Second-generation shRNA libraries covering the mouse and human genomes
    Jose M Silva
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Genet 37:1281-8. 2005
    ..These libraries are available to the scientific community...
  13. ncbi request reprint Probing tumor phenotypes using stable and regulated synthetic microRNA precursors
    Ross A Dickins
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Nat Genet 37:1289-95. 2005
    ..In practice, this primary microRNA-based short hairpin RNA vector system is markedly similar to cDNA overexpression systems and is a powerful tool for studying gene function in cells and animals...
  14. ncbi request reprint Tissue-specific and reversible RNA interference in transgenic mice
    Ross A Dickins
    Howard Hughes Medical Institute, Cold Spring Harbor, New York 11724, USA
    Nat Genet 39:914-21. 2007
    ..By leaving the target gene unaltered, this approach permits tissue-specific, reversible regulation of endogenous gene expression in vivo, with potential broad application in basic biology and drug target validation...
  15. pmc DNA Sudoku--harnessing high-throughput sequencing for multiplexed specimen analysis
    Yaniv Erlich
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Genome Res 19:1243-53. 2009
    ..We achieved greater than 97% accuracy in these trials. The strategies reported here can be applied to a wide variety of biological problems, including the determination of genotypic variation within large populations of individuals...
  16. pmc Conservation of small RNA pathways in platypus
    Elizabeth P Murchison
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Genome Res 18:995-1004. 2008
    ..Platypus and echidna testes contain a robust Piwi-interacting (piRNA) system, which appears to be participating in ongoing transposon defense...
  17. ncbi request reprint A microRNA polycistron as a potential human oncogene
    Lin He
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 435:828-33. 2005
    ..Together, these studies indicate that non-coding RNAs, specifically microRNAs, can modulate tumour formation, and implicate the mir-17-92 cluster as a potential human oncogene...
  18. ncbi request reprint A resource for large-scale RNA-interference-based screens in mammals
    Patrick J Paddison
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 428:427-31. 2004
    ..Our results suggest that our large-scale RNAi library can be used in specific, genetic applications in mammals, and will become a valuable resource for gene analysis and discovery...
  19. ncbi request reprint Biochemical specialization within Arabidopsis RNA silencing pathways
    Yijun Qi
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Mol Cell 19:421-8. 2005
    ..We find that specific members of the Dicer and Argonaute families have distinct biochemical activities, which provides insight into their roles within RNA silencing pathways in Arabidopsis...
  20. ncbi request reprint Lessons from Nature: microRNA-based shRNA libraries
    Kenneth Chang
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Howard Hughes Medical Institute, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Methods 3:707-14. 2006
    ..Library cassettes can be easily shuttled into vectors that contain different promoters and/or that provide different modes of viral delivery...
  21. ncbi request reprint A microRNA component of the p53 tumour suppressor network
    Lin He
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 447:1130-4. 2007
    ..The p53 network suppresses tumour formation through the coordinated activation of multiple transcriptional targets, and miR-34 may act in concert with other effectors to inhibit inappropriate cell proliferation...
  22. ncbi request reprint RNase III enzymes and the initiation of gene silencing
    Michelle A Carmell
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Struct Mol Biol 11:214-8. 2004
    ..Recently, a related RNAse III enzyme, Drosha, has surfaced as another component of the RNAi pathway. In addition to biochemistry, protein structures have proven to be helpful in deciphering the enzymology of RNase III molecules...
  23. pmc A dicer-independent miRNA biogenesis pathway that requires Ago catalysis
    Sihem Cheloufi
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor, New York 11724, USA
    Nature 465:584-9. 2010
    ..Our findings link the conservation of Argonaute catalysis to a conserved mechanism of microRNA biogenesis that is important for vertebrate development...
  24. pmc Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members
    Vasily V Vagin
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Genes Dev 23:1749-62. 2009
    ....
  25. pmc shutdown is a component of the Drosophila piRNA biogenesis machinery
    Jonathan B Preall
    Howard Hughes Medical Institute, Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    RNA 18:1446-57. 2012
    ..Shu contains domains implicated in peptidyl-prolyl cis-trans isomerase activity and in the binding of HSP90-family chaperones, though the relevance of these domains to piRNA biogenesis is unknown...
  26. ncbi request reprint Cloning of short hairpin RNAs for gene knockdown in mammalian cells
    Patrick J Paddison
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Methods 1:163-7. 2004
  27. ncbi request reprint MicroRNAs: small RNAs with a big role in gene regulation
    Lin He
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Rev Genet 5:522-31. 2004
  28. ncbi request reprint The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes
    Ji Joon Song
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Struct Biol 10:1026-32. 2003
    ....
  29. ncbi request reprint A germline-specific class of small RNAs binds mammalian Piwi proteins
    Angelique Girard
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 442:199-202. 2006
    ..Although their function must still be resolved, the abundance of piRNAs in germline cells and the male sterility of Miwi mutants suggest a role in gametogenesis...
  30. ncbi request reprint Dicer is essential for mouse development
    Emily Bernstein
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Genet 35:215-7. 2003
    ..Coupled with our inability to generate viable Dicer1-null embryonic stem (ES) cells, this suggests a role for Dicer, and, by implication, the RNAi machinery, in maintaining the stem cell population during early mouse development...
  31. pmc The structural biochemistry of Zucchini implicates it as a nuclease in piRNA biogenesis
    Jonathan J Ipsaro
    W M Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Nature 491:279-83. 2012
    ..Together, our data suggest that the Zucchini proteins act in primary piRNA biogenesis as nucleases, perhaps generating the 5' ends of primary piRNAs...
  32. pmc A role for microRNAs in maintenance of mouse mammary epithelial progenitor cells
    Ingrid Ibarra
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Genes Dev 21:3238-43. 2007
    ..Let-7 sensors can be used to prospectively enrich self-renewing populations, and enforced let-7 expression induces loss of self-renewing cells from mixed cultures...
  33. pmc An oncogenomics-based in vivo RNAi screen identifies tumor suppressors in liver cancer
    Lars Zender
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Cell 135:852-64. 2008
    ..Our results establish the feasibility of in vivo RNAi screens and illustrate how combining cancer genomics, RNA interference, and mosaic mouse models can facilitate the functional annotation of the cancer genome...
  34. ncbi request reprint Distinct catalytic and non-catalytic roles of ARGONAUTE4 in RNA-directed DNA methylation
    Yijun Qi
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 443:1008-12. 2006
    ..Second, AGO4 catalytic activity can be crucial for the generation of secondary siRNAs that reinforce its repressive effects...
  35. pmc Ancestral roles of small RNAs: an Ago-centric perspective
    Leemor Joshua-Tor
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Cold Spring Harb Perspect Biol 3:a003772. 2011
    ..Here, we discuss the diversity of Argonaute proteins from a structural and functional perspective...
  36. pmc Production of artificial piRNAs in flies and mice
    Felix Muerdter
    Howard Hughes Medical Institute, Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    RNA 18:42-52. 2012
    ....
  37. ncbi request reprint Genome-wide in situ exon capture for selective resequencing
    Emily Hodges
    Howard Hughes Medical Institute, Watson School of Biological Sciences, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Genet 39:1522-7. 2007
    ..This methodology provides an adaptable route toward rapid and efficient resequencing of any sizeable, non-repeat portion of the human genome...
  38. ncbi request reprint Germline transmission of RNAi in mice
    Michelle A Carmell
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, New York 11724, USA
    Nat Struct Biol 10:91-2. 2003
  39. ncbi request reprint A micrococcal nuclease homologue in RNAi effector complexes
    Amy A Caudy
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 425:411-4. 2003
    ..Tudor-SN is the first RISC subunit to be identified that contains a recognizable nuclease domain, and could therefore contribute to the RNA degradation observed in RNAi...
  40. pmc Alta-Cyclic: a self-optimizing base caller for next-generation sequencing
    Yaniv Erlich
    Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Methods 5:679-82. 2008
    ..Alta-Cyclic substantially improved the number of accurate reads for sequencing runs up to 78 bases and reduced systematic biases, facilitating confident identification of sequence variants...
  41. ncbi request reprint An epi-allelic series of p53 hypomorphs created by stable RNAi produces distinct tumor phenotypes in vivo
    Michael T Hemann
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor, New York 11724, USA
    Nat Genet 33:396-400. 2003
    ..In addition, intrinsic differences between individual shRNA expression vectors targeting the same gene can be used to create an 'epi-allelic series' for dissecting gene function in vivo...
  42. pmc Small RNA sorting: matchmaking for Argonautes
    Benjamin Czech
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Rev Genet 12:19-31. 2011
    ....
  43. ncbi request reprint Unlocking the potential of the human genome with RNA interference
    Gregory J Hannon
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 431:371-8. 2004
    ..Harnessed as an experimental tool, RNAi has revolutionized approaches to decoding gene function. It also has the potential to be exploited therapeutically, and clinical trials to test this possibility are already being planned...
  44. pmc Multiple roles for Piwi in silencing Drosophila transposons
    Nikolay V Rozhkov
    Watson School of Biological Sciences
    Genes Dev 27:400-12. 2013
    ..Overall, our results indicate that Piwi plays multiple roles in the piRNA pathway, in part enforcing transposon repression through effects on local chromatin states and transcription but also participating in germ cell piRNA biogenesis...
  45. pmc Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells
    Patrick J Paddison
    Watson School of Biological Sciences, Cold Spring Harbor, New York 11724, USA
    Genes Dev 16:948-58. 2002
    ....
  46. ncbi request reprint The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis
    Michelle A Carmell
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Genes Dev 16:2733-42. 2002
  47. pmc Genome analysis of the platypus reveals unique signatures of evolution
    Wesley C Warren
    Genome Sequencing Center, Washington University School of Medicine, Campus Box 8501, 4444 Forest Park Avenue, St Louis, Missouri 63108, USA
    Nature 453:175-83. 2008
    ..Sequencing of this genome now provides a valuable resource for deep mammalian comparative analyses, as well as for monotreme biology and conservation...
  48. pmc Inducible, reversible, and stable RNA interference in mammalian cells
    Sunita Gupta
    Cancer Genome Research Center, Cold Spring Harbor Laboratory, 500 Sunnyside Boulevard, Woodbury, NY 11797, USA
    Proc Natl Acad Sci U S A 101:1927-32. 2004
    ..Inducible and reversible regulation of RNA interference has broad applications in the areas of mammalian genetics and molecular therapeutics...
  49. pmc A piRNA pathway primed by individual transposons is linked to de novo DNA methylation in mice
    Alexei A Aravin
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Mol Cell 31:785-99. 2008
    ..This implicates piRNAs as specificity determinants of DNA methylation in germ cells...
  50. ncbi request reprint Free energy lights the path toward more effective RNAi
    Jose M Silva
    Nat Genet 35:303-5. 2003
  51. ncbi request reprint siRNAs and shRNAs: skeleton keys to the human genome
    Patrick J Paddison
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, McClintock Building, 1 Bungtown Road, Cold Spring Harbor, NY 1172, USA
    Curr Opin Mol Ther 5:217-24. 2003
    ..In this review, some of the key features of RNAi in mammalian systems are discussed...
  52. ncbi request reprint Rb-mediated heterochromatin formation and silencing of E2F target genes during cellular senescence
    Masashi Narita
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Cell 113:703-16. 2003
    ..These results provide a molecular explanation for the stability of the senescent state, as well as new insights into the action of Rb as a tumor suppressor...
  53. ncbi request reprint RNAi: an ever-growing puzzle
    Ahmet M Denli
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor, NY 11724, USA
    Trends Biochem Sci 28:196-201. 2003
    ....
  54. ncbi request reprint RNA interference: a promising approach to antiviral therapy?
    Jose M Silva
    Trends Mol Med 8:505-8. 2002
  55. pmc Fragile X-related protein and VIG associate with the RNA interference machinery
    Amy A Caudy
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor, NY 11724, USA
    Genes Dev 16:2491-6. 2002
    ..The possibility that dFXR, and potentially FMRP, use, at least in part, an RNAi-related mechanism for target recognition suggests a potentially important link between RNAi and human disease...
  56. ncbi request reprint RNA interference in adult mice
    Anton P McCaffrey
    Department of Pediatrics, Stanford University School of Medicine, Stanford, California 94305 5208, USA
    Nature 418:38-9. 2002
    ..We also show the therapeutic potential of this technique by demonstrating effective targeting of a sequence from hepatitis C virus by RNA interference in vivo...
  57. ncbi request reprint RNA interference by short hairpin RNAs expressed in vertebrate cells
    Gregory J Hannon
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA
    Methods Mol Biol 257:255-66. 2004
    ..We describe methods for the construction and transfer of stable shRNA expressing vectors suitable for generating loss of function alleles in mammalian cells in vitro or in vivo...
  58. ncbi request reprint RNAi and expression of retrotransposons MuERV-L and IAP in preimplantation mouse embryos
    Petr Svoboda
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104 6018, USA
    Dev Biol 269:276-85. 2004
    ....
  59. pmc A role for the P-body component GW182 in microRNA function
    Jidong Liu
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Howard Hughes Medical Institute, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Nat Cell Biol 7:1261-6. 2005
    ..Thus, our results support a functional link between cytoplasmic P-bodies and the ability of a microRNA to repress expression of a target mRNA...
  60. ncbi request reprint A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in Zebrafish
    Saskia Houwing
    Hubrecht Laboratory, Uppsalalaan 8, Utrecht, Netherlands
    Cell 129:69-82. 2007
    ..Furthermore, we show that piRNAs are Dicer independent and that their 3' end likely carries a 2'O-Methyl modification...
  61. ncbi request reprint Uncovering RNAi mechanisms in plants: biochemistry enters the foray
    Yijun Qi
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    FEBS Lett 579:5899-903. 2005
    ..In this review, we discuss the recent developments in our understanding of plant RNAi mechanisms from a biochemical perspective...
  62. ncbi request reprint Argonaute2 is the catalytic engine of mammalian RNAi
    Jidong Liu
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Science 305:1437-41. 2004
    ..Thus, our evidence supports a model in which Argonaute contributes "Slicer" activity to RISC, providing the catalytic engine for RNAi...
  63. ncbi request reprint The Piwi-piRNA pathway provides an adaptive defense in the transposon arms race
    Alexei A Aravin
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Science 318:761-4. 2007
    ..A likely function of RNAi throughout that history is to protect the genome from both pathogenic and parasitic invaders...
  64. pmc Suppression of p160ROCK bypasses cell cycle arrest after Aurora-A/STK15 depletion
    Jian Du
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 101:8975-80. 2004
    ..These results suggest that Aurora-A and p160ROCK act in a common genetic pathway that promotes and monitors progression through G(2)/M...
  65. pmc Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes
    Alexander Stark
    Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02141, USA
    Genome Res 17:1865-79. 2007
    ..For mir-10 in particular, both arms show abundant processing, and both show highly conserved target sites in Hox genes, suggesting a possible cooperation of the two arms, and their role as a master Hox regulator...
  66. ncbi request reprint Short hairpin activated gene silencing in mammalian cells
    Patrick J Paddison
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor, NY, USA
    Methods Mol Biol 265:85-100. 2004
    ..We also provide methods that use shRNAs to permit different levels of gene expression. Additionally, we discuss some aspects important for constructing an information pipeline to support development of a large shRNA library...
  67. pmc Developmentally regulated cleavage of tRNAs in the bacterium Streptomyces coelicolor
    Henry J Haiser
    Department of Biology, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada
    Nucleic Acids Res 36:732-41. 2008
    ..Mutants defective in aerial development and antibiotic production exhibit altered tRNA cleavage profiles relative to wild-type strains...
  68. pmc Functionally distinct regulatory RNAs generated by bidirectional transcription and processing of microRNA loci
    David M Tyler
    Department of Developmental Biology, Sloan Kettering Institute, New York, New York 10021, USA
    Genes Dev 22:26-36. 2008
    ..These findings demonstrate that antisense transcription and processing contributes to the functional diversification of miRNA genes...
  69. pmc Conserved themes in small-RNA-mediated transposon control
    Angelique Girard
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Trends Cell Biol 18:136-48. 2008
    ....
  70. ncbi request reprint Induction and biochemical purification of RNA-induced silencing complex from Drosophila S2 cells
    Amy A Caudy
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor, NY, USA
    Methods Mol Biol 265:59-72. 2004
    ..Purified RISC complexes contain short interfering RNAs and endogenously expressed miRNAs and will be useful for studying many aspects of the RNAi machinery...
  71. ncbi request reprint Control of translation and mRNA degradation by miRNAs and siRNAs
    Marco Antonio Valencia-Sanchez
    Department of Molecular and Cellular Biology, Howard Hughes Medical Institute, University of Arizona, Tucson, Arizona 85721, USA
    Genes Dev 20:515-24. 2006
    ..An emerging theme is that miRNAs, and siRNAs to some extent, target mRNAs to the general eukaryotic machinery for mRNA degradation and translation control...