Gregory Hannon

Summary

Affiliation: Cold Spring Harbor Laboratory
Country: USA

Publications

  1. pmc The structural biochemistry of Zucchini implicates it as a nuclease in piRNA biogenesis
    Jonathan J Ipsaro
    W M Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Nature 491:279-83. 2012
  2. pmc Cytoplasmic compartmentalization of the fetal piRNA pathway in mice
    Alexei A Aravin
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA
    PLoS Genet 5:e1000764. 2009
  3. pmc Unique small RNA signatures uncovered in the tammar wallaby genome
    James Lindsay
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA
    BMC Genomics 13:559. 2012
  4. ncbi request reprint Unlocking the potential of the human genome with RNA interference
    Gregory J Hannon
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 431:371-8. 2004
  5. pmc A complex system of small RNAs in the unicellular green alga Chlamydomonas reinhardtii
    Tao Zhao
    National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China
    Genes Dev 21:1190-203. 2007
  6. ncbi request reprint Probing tumor phenotypes using stable and regulated synthetic microRNA precursors
    Ross A Dickins
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Nat Genet 37:1289-95. 2005
  7. ncbi request reprint Uncovering RNAi mechanisms in plants: biochemistry enters the foray
    Yijun Qi
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    FEBS Lett 579:5899-903. 2005
  8. ncbi request reprint Biochemical specialization within Arabidopsis RNA silencing pathways
    Yijun Qi
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Mol Cell 19:421-8. 2005
  9. ncbi request reprint RNA-interference-directed chromatin modification coupled to RNA polymerase II transcription
    Vera Schramke
    Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, King s Buildings, University of Edinburgh, Edinburgh EH9 3JR, UK
    Nature 435:1275-9. 2005
  10. ncbi request reprint Argonaute2 is the catalytic engine of mammalian RNAi
    Jidong Liu
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Science 305:1437-41. 2004

Research Grants

  1. MECHANISMS OF DSRNA-INDUCED GENE SILENCING
    Gregory Hannon; Fiscal Year: 2001
  2. MECHANISMS OF DSRNA-INDUCED GENE SILENCING
    Gregory Hannon; Fiscal Year: 2009
  3. MECHANISMS OF DSRNA-INDUCED GENE SILENCING
    Gregory Hannon; Fiscal Year: 2007
  4. COLD SPRING HARBOR LABORATORY CANCER RESEARCH CENTER
    Gregory Hannon; Fiscal Year: 2007
  5. MECHANISMS OF DSRNA-INDUCED GENE SILENCING
    Gregory Hannon; Fiscal Year: 2006
  6. MECHANISMS OF DSRNA-INDUCED GENE SILENCING
    Gregory Hannon; Fiscal Year: 2005
  7. MECHANISMS OF DSRNA-INDUCED GENE SILENCING
    Gregory Hannon; Fiscal Year: 2004
  8. Conference on Roles of RNA in Gene Regulation
    Gregory Hannon; Fiscal Year: 2005
  9. MECHANISMS OF DSRNA-INDUCED GENE SILENCING
    Gregory Hannon; Fiscal Year: 2003
  10. MECHANISMS OF DSRNA-INDUCED GENE SILENCING
    Gregory Hannon; Fiscal Year: 2004

Collaborators

Detail Information

Publications65

  1. pmc The structural biochemistry of Zucchini implicates it as a nuclease in piRNA biogenesis
    Jonathan J Ipsaro
    W M Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Nature 491:279-83. 2012
    ..Together, our data suggest that the Zucchini proteins act in primary piRNA biogenesis as nucleases, perhaps generating the 5' ends of primary piRNAs...
  2. pmc Cytoplasmic compartmentalization of the fetal piRNA pathway in mice
    Alexei A Aravin
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA
    PLoS Genet 5:e1000764. 2009
    ..Cumulatively, our data reveal elaborate cytoplasmic compartmentalization of the fetal piRNA pathway that relies on MAEL function...
  3. pmc Unique small RNA signatures uncovered in the tammar wallaby genome
    James Lindsay
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA
    BMC Genomics 13:559. 2012
    ....
  4. ncbi request reprint Unlocking the potential of the human genome with RNA interference
    Gregory J Hannon
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 431:371-8. 2004
    ..Harnessed as an experimental tool, RNAi has revolutionized approaches to decoding gene function. It also has the potential to be exploited therapeutically, and clinical trials to test this possibility are already being planned...
  5. pmc A complex system of small RNAs in the unicellular green alga Chlamydomonas reinhardtii
    Tao Zhao
    National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China
    Genes Dev 21:1190-203. 2007
    ..Our findings suggest that the miRNA pathway and some siRNA pathways are ancient mechanisms of gene regulation that evolved prior to the emergence of multicellularity...
  6. ncbi request reprint Probing tumor phenotypes using stable and regulated synthetic microRNA precursors
    Ross A Dickins
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Nat Genet 37:1289-95. 2005
    ..In practice, this primary microRNA-based short hairpin RNA vector system is markedly similar to cDNA overexpression systems and is a powerful tool for studying gene function in cells and animals...
  7. ncbi request reprint Uncovering RNAi mechanisms in plants: biochemistry enters the foray
    Yijun Qi
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    FEBS Lett 579:5899-903. 2005
    ..In this review, we discuss the recent developments in our understanding of plant RNAi mechanisms from a biochemical perspective...
  8. ncbi request reprint Biochemical specialization within Arabidopsis RNA silencing pathways
    Yijun Qi
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Mol Cell 19:421-8. 2005
    ..We find that specific members of the Dicer and Argonaute families have distinct biochemical activities, which provides insight into their roles within RNA silencing pathways in Arabidopsis...
  9. ncbi request reprint RNA-interference-directed chromatin modification coupled to RNA polymerase II transcription
    Vera Schramke
    Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, King s Buildings, University of Edinburgh, Edinburgh EH9 3JR, UK
    Nature 435:1275-9. 2005
    ..Truncation of the regulatory carboxy-terminal domain (CTD) of RNA pol II disrupts transcriptional silencing, indicating that, like other RNA processing events, RNAi-directed chromatin modification is coupled to transcription...
  10. ncbi request reprint Argonaute2 is the catalytic engine of mammalian RNAi
    Jidong Liu
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Science 305:1437-41. 2004
    ..Thus, our evidence supports a model in which Argonaute contributes "Slicer" activity to RISC, providing the catalytic engine for RNAi...
  11. ncbi request reprint Second-generation shRNA libraries covering the mouse and human genomes
    Jose M Silva
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Genet 37:1281-8. 2005
    ..These libraries are available to the scientific community...
  12. ncbi request reprint Control of translation and mRNA degradation by miRNAs and siRNAs
    Marco Antonio Valencia-Sanchez
    Department of Molecular and Cellular Biology, Howard Hughes Medical Institute, University of Arizona, Tucson, Arizona 85721, USA
    Genes Dev 20:515-24. 2006
    ..An emerging theme is that miRNAs, and siRNAs to some extent, target mRNAs to the general eukaryotic machinery for mRNA degradation and translation control...
  13. ncbi request reprint Distinct catalytic and non-catalytic roles of ARGONAUTE4 in RNA-directed DNA methylation
    Yijun Qi
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 443:1008-12. 2006
    ..Second, AGO4 catalytic activity can be crucial for the generation of secondary siRNAs that reinforce its repressive effects...
  14. pmc Critical roles for Dicer in the female germline
    Elizabeth P Murchison
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, Cold Spring Harbor, New York 11724, USA
    Genes Dev 21:682-93. 2007
    ..Our studies identify Dicer as central to a regulatory network that controls oocyte gene expression programs and that promotes genomic integrity in a cell type notoriously susceptible to aneuploidy...
  15. pmc Profiling essential genes in human mammary cells by multiplex RNAi screening
    Jose M Silva
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Science 319:617-20. 2008
    ..These studies establish a practical platform for genome-scale screening of complex phenotypes in mammalian cells and demonstrate that RNAi can be used to expose genotype-specific sensitivities...
  16. pmc Cancer proliferation gene discovery through functional genomics
    Michael R Schlabach
    Howard Hughes Medical Institute and Department of Genetics, Center for Genetics and Genomics, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Science 319:620-4. 2008
    ..Such efforts are complementary to the Cancer Genome Atlas and provide an alternative functional view of cancer cells...
  17. pmc Conserved themes in small-RNA-mediated transposon control
    Angelique Girard
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Trends Cell Biol 18:136-48. 2008
    ....
  18. pmc Sorting of small RNAs into Arabidopsis argonaute complexes is directed by the 5' terminal nucleotide
    Shijun Mi
    National Institute of Biological Sciences, No 7 Science Park Road, Zhongguancun Life Science Park, Beijing 102206, China
    Cell 133:116-27. 2008
    ..This suggests that specialization of AGO complexes might involve remodeling the 5' end-binding pocket to accept certain small RNA sequences, perhaps explaining the evolutionary drive for miRNAs to initiate with uridine...
  19. pmc Pseudogene-derived small interfering RNAs regulate gene expression in mouse oocytes
    Oliver H Tam
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 453:534-8. 2008
    ..Our findings indicate a function for pseudogenes in regulating gene expression by means of the RNA interference pathway and may, in part, explain the evolutionary pressure to conserve argonaute-mediated catalysis in mammals...
  20. ncbi request reprint Induction and biochemical purification of RNA-induced silencing complex from Drosophila S2 cells
    Amy A Caudy
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor, NY, USA
    Methods Mol Biol 265:59-72. 2004
    ..Purified RISC complexes contain short interfering RNAs and endogenously expressed miRNAs and will be useful for studying many aspects of the RNAi machinery...
  21. ncbi request reprint RNAi: an ever-growing puzzle
    Ahmet M Denli
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor, NY 11724, USA
    Trends Biochem Sci 28:196-201. 2003
    ....
  22. ncbi request reprint siRNAs and shRNAs: skeleton keys to the human genome
    Patrick J Paddison
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, McClintock Building, 1 Bungtown Road, Cold Spring Harbor, NY 1172, USA
    Curr Opin Mol Ther 5:217-24. 2003
    ..In this review, some of the key features of RNAi in mammalian systems are discussed...
  23. ncbi request reprint RNase III enzymes and the initiation of gene silencing
    Michelle A Carmell
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Struct Mol Biol 11:214-8. 2004
    ..Recently, a related RNAse III enzyme, Drosha, has surfaced as another component of the RNAi pathway. In addition to biochemistry, protein structures have proven to be helpful in deciphering the enzymology of RNase III molecules...
  24. ncbi request reprint Free energy lights the path toward more effective RNAi
    Jose M Silva
    Nat Genet 35:303-5. 2003
  25. ncbi request reprint RNA interference: a promising approach to antiviral therapy?
    Jose M Silva
    Trends Mol Med 8:505-8. 2002
  26. ncbi request reprint RNA interference by short hairpin RNAs expressed in vertebrate cells
    Gregory J Hannon
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA
    Methods Mol Biol 257:255-66. 2004
    ..We describe methods for the construction and transfer of stable shRNA expressing vectors suitable for generating loss of function alleles in mammalian cells in vitro or in vivo...
  27. pmc Stable suppression of gene expression by RNAi in mammalian cells
    Patrick J Paddison
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 99:1443-8. 2002
    ....
  28. pmc Fragile X-related protein and VIG associate with the RNA interference machinery
    Amy A Caudy
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor, NY 11724, USA
    Genes Dev 16:2491-6. 2002
    ..The possibility that dFXR, and potentially FMRP, use, at least in part, an RNAi-related mechanism for target recognition suggests a potentially important link between RNAi and human disease...
  29. ncbi request reprint RNA interference: the new somatic cell genetics?
    Patrick J Paddison
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Cancer Cell 2:17-23. 2002
    ....
  30. ncbi request reprint RNAi and expression of retrotransposons MuERV-L and IAP in preimplantation mouse embryos
    Petr Svoboda
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104 6018, USA
    Dev Biol 269:276-85. 2004
    ....
  31. ncbi request reprint RNA interference in adult mice
    Anton P McCaffrey
    Department of Pediatrics, Stanford University School of Medicine, Stanford, California 94305 5208, USA
    Nature 418:38-9. 2002
    ..We also show the therapeutic potential of this technique by demonstrating effective targeting of a sequence from hepatitis C virus by RNA interference in vivo...
  32. ncbi request reprint RNA interference
    Gregory J Hannon
    Cold Spring Harbour Laboratory, New York 11724, USA
    Nature 418:244-51. 2002
    ..RNAi has been cultivated as a means to manipulate gene expression experimentally and to probe gene function on a whole-genome scale...
  33. pmc Bcl2L12 inhibits post-mitochondrial apoptosis signaling in glioblastoma
    Alexander H Stegh
    Department of Medical Oncology, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Genes Dev 21:98-111. 2007
    ..Thus, Bcl2L12 contributes to the classical tumor biological features of GBM such as intense apoptosis resistance and florid necrosis, and may provide a target for enhanced therapeutic responsiveness of this lethal cancer...
  34. ncbi request reprint An epi-allelic series of p53 hypomorphs created by stable RNAi produces distinct tumor phenotypes in vivo
    Michael T Hemann
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor, New York 11724, USA
    Nat Genet 33:396-400. 2003
    ..In addition, intrinsic differences between individual shRNA expression vectors targeting the same gene can be used to create an 'epi-allelic series' for dissecting gene function in vivo...
  35. ncbi request reprint A functional genomic screen identifies a role for TAO1 kinase in spindle-checkpoint signalling
    Viji M Draviam
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Nat Cell Biol 9:556-64. 2007
    ....
  36. pmc Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells
    Patrick J Paddison
    Watson School of Biological Sciences, Cold Spring Harbor, New York 11724, USA
    Genes Dev 16:948-58. 2002
    ....
  37. ncbi request reprint A microRNA component of the p53 tumour suppressor network
    Lin He
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 447:1130-4. 2007
    ..The p53 network suppresses tumour formation through the coordinated activation of multiple transcriptional targets, and miR-34 may act in concert with other effectors to inhibit inappropriate cell proliferation...
  38. ncbi request reprint The Piwi-piRNA pathway provides an adaptive defense in the transposon arms race
    Alexei A Aravin
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Science 318:761-4. 2007
    ..A likely function of RNAi throughout that history is to protect the genome from both pathogenic and parasitic invaders...
  39. pmc A piRNA pathway primed by individual transposons is linked to de novo DNA methylation in mice
    Alexei A Aravin
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Mol Cell 31:785-99. 2008
    ..This implicates piRNAs as specificity determinants of DNA methylation in germ cells...
  40. ncbi request reprint Genome-wide in situ exon capture for selective resequencing
    Emily Hodges
    Howard Hughes Medical Institute, Watson School of Biological Sciences, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Genet 39:1522-7. 2007
    ..This methodology provides an adaptable route toward rapid and efficient resequencing of any sizeable, non-repeat portion of the human genome...
  41. ncbi request reprint The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis
    Michelle A Carmell
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Genes Dev 16:2733-42. 2002
  42. pmc Topoisomerase levels determine chemotherapy response in vitro and in vivo
    Darren J Burgess
    Cold Spring Harbor Laboratory and Howard Hughes Medical Institute, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 105:9053-8. 2008
    ..These results highlight the utility of pooled shRNA screens for identifying genetic determinants of chemotherapy response and suggest strategies for improving the effectiveness of topoisomerase poisons in the clinic...
  43. pmc An endogenous small interfering RNA pathway in Drosophila
    Benjamin Czech
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 453:798-802. 2008
    ..These observations expand the repertoire of small RNAs in Drosophila, adding a class that blurs distinctions based on known biogenesis mechanisms and functional roles...
  44. pmc Conservation of small RNA pathways in platypus
    Elizabeth P Murchison
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Genome Res 18:995-1004. 2008
    ..Platypus and echidna testes contain a robust Piwi-interacting (piRNA) system, which appears to be participating in ongoing transposon defense...
  45. pmc Targeted deletion of Dicer in the heart leads to dilated cardiomyopathy and heart failure
    Jian Fu Chen
    Carolina Cardiovascular Biology Center, Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 105:2111-6. 2008
    ..Together, our studies demonstrate essential roles for Dicer in cardiac contraction and indicate that miRNAs play critical roles in normal cardiac function and under pathological conditions...
  46. ncbi request reprint Lessons from Nature: microRNA-based shRNA libraries
    Kenneth Chang
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Howard Hughes Medical Institute, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Methods 3:707-14. 2006
    ..Library cassettes can be easily shuttled into vectors that contain different promoters and/or that provide different modes of viral delivery...
  47. pmc MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies
    Jidong Liu
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Nat Cell Biol 7:719-23. 2005
    ..These results provide a link between miRNA function and mammalian P-bodies and suggest that translation repression by RISC delivers mRNAs to P-bodies, either as a cause or as a consequence of inhibiting protein synthesis...
  48. ncbi request reprint Purified Argonaute2 and an siRNA form recombinant human RISC
    Fabiola V Rivas
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, New York 11724, USA
    Nat Struct Mol Biol 12:340-9. 2005
    ..These studies demonstrate that Argonaute proteins catalyze mRNA cleavage within RISC and provide a source of recombinant enzyme for detailed biochemical studies of the RNAi effector complex...
  49. ncbi request reprint Synthetic shRNAs as potent RNAi triggers
    Despina Siolas
    Nat Biotechnol 23:227-31. 2005
    ..Maximal inhibition of target genes was achieved at lower concentrations and silencing at 24 h was often greater. These studies provide the basis for an improved approach to triggering experimental silencing via the RNAi pathway...
  50. ncbi request reprint RNA-interference-based functional genomics in mammalian cells: reverse genetics coming of age
    Jose Silva
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Oncogene 23:8401-9. 2004
    ....
  51. ncbi request reprint A resource for large-scale RNA-interference-based screens in mammals
    Patrick J Paddison
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 428:427-31. 2004
    ..Our results suggest that our large-scale RNAi library can be used in specific, genetic applications in mammals, and will become a valuable resource for gene analysis and discovery...
  52. ncbi request reprint Crystal structure of Argonaute and its implications for RISC slicer activity
    Ji Joon Song
    Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Science 305:1434-7. 2004
    ..The architecture of the molecule and the placement of the PAZ and PIWI domains define a groove for substrate binding and suggest a mechanism for siRNA-guided mRNA cleavage...
  53. pmc Suppression of p160ROCK bypasses cell cycle arrest after Aurora-A/STK15 depletion
    Jian Du
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 101:8975-80. 2004
    ..These results suggest that Aurora-A and p160ROCK act in a common genetic pathway that promotes and monitors progression through G(2)/M...
  54. ncbi request reprint Short hairpin activated gene silencing in mammalian cells
    Patrick J Paddison
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor, NY, USA
    Methods Mol Biol 265:85-100. 2004
    ..We also provide methods that use shRNAs to permit different levels of gene expression. Additionally, we discuss some aspects important for constructing an information pipeline to support development of a large shRNA library...
  55. ncbi request reprint A genetic screen for candidate tumor suppressors identifies REST
    Thomas F Westbrook
    Howard Hughes Medical Institute, Department of Genetics, Harvard Partners Center for Genetics and Genomics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Cell 121:837-48. 2005
    ..These results implicate REST as a human tumor suppressor and provide a novel approach to identifying candidate genes that suppress the development of human cancer...
  56. ncbi request reprint Germline transmission of RNAi in mice
    Michelle A Carmell
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, New York 11724, USA
    Nat Struct Biol 10:91-2. 2003
  57. pmc The miRNA-processing enzyme dicer is essential for the morphogenesis and maintenance of hair follicles
    Thomas Andl
    Departments of Dermatology and Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Curr Biol 16:1041-9. 2006
    ..These results reveal critical roles for Dicer in the skin and implicate miRNAs in key aspects of epidermal and hair-follicle development and function...
  58. pmc Suppression of prion protein in livestock by RNA interference
    Michael C Golding
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Howard Hughes Medical Institute, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 103:5285-90. 2006
    ....
  59. pmc A role for the P-body component GW182 in microRNA function
    Jidong Liu
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Howard Hughes Medical Institute, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Nat Cell Biol 7:1261-6. 2005
    ..Thus, our results support a functional link between cytoplasmic P-bodies and the ability of a microRNA to repress expression of a target mRNA...
  60. ncbi request reprint A micrococcal nuclease homologue in RNAi effector complexes
    Amy A Caudy
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 425:411-4. 2003
    ..Tudor-SN is the first RISC subunit to be identified that contains a recognizable nuclease domain, and could therefore contribute to the RNA degradation observed in RNAi...
  61. ncbi request reprint The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes
    Ji Joon Song
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Struct Biol 10:1026-32. 2003
    ....
  62. ncbi request reprint Cloning of short hairpin RNAs for gene knockdown in mammalian cells
    Patrick J Paddison
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Methods 1:163-7. 2004
  63. pmc A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells
    Frank Stegmeier
    Harvard University Medical School, Department of Genetics, Center for Genetics and Genomics, Howard Hughes Medical Institute, and Brigham and Women s Hospital, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:13212-7. 2005
    ..The high penetrance of these vectors will facilitate genomewide loss-of-function screens and is an important step toward using bar-coding strategies to follow loss of specific sequences in complex populations...
  64. pmc Characterization of Dicer-deficient murine embryonic stem cells
    Elizabeth P Murchison
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 102:12135-40. 2005
    ..These studies provide a conditional model of RNAi deficiency in mammals that will permit the dissection of the biological roles of the RNAi machinery in cultured mammalian cells...
  65. pmc RNA interference microarrays: high-throughput loss-of-function genetics in mammalian cells
    Jose M Silva
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 101:6548-52. 2004
    ..We have validated this approach by targeting genes involved in cytokinesis and proteasome-mediated proteolysis...

Research Grants17

  1. MECHANISMS OF DSRNA-INDUCED GENE SILENCING
    Gregory Hannon; Fiscal Year: 2001
    ..In this application, we outline our approach toward elucidating the mechanisms underlying dsRNA-induced gene silencing through a combination of in vitro and in vivo studies. ..
  2. MECHANISMS OF DSRNA-INDUCED GENE SILENCING
    Gregory Hannon; Fiscal Year: 2009
    ..They serve as tools for understanding normal and aberrant biological processes and will perhaps transform clinical practice by serving as the basis for a new generation of therapeutic agents. ..
  3. MECHANISMS OF DSRNA-INDUCED GENE SILENCING
    Gregory Hannon; Fiscal Year: 2007
    ....
  4. COLD SPRING HARBOR LABORATORY CANCER RESEARCH CENTER
    Gregory Hannon; Fiscal Year: 2007
    ..The findings from this Program have the potential both to inform the effective application of current therapies and to identify proteins and networks that may become targets for the development of new therapeutic agents. ..
  5. MECHANISMS OF DSRNA-INDUCED GENE SILENCING
    Gregory Hannon; Fiscal Year: 2006
    ....
  6. MECHANISMS OF DSRNA-INDUCED GENE SILENCING
    Gregory Hannon; Fiscal Year: 2005
    ....
  7. MECHANISMS OF DSRNA-INDUCED GENE SILENCING
    Gregory Hannon; Fiscal Year: 2004
    ..In this application, we outline our approach toward elucidating the mechanisms underlying dsRNA-induced gene silencing through a combination of in vitro and in vivo studies. ..
  8. Conference on Roles of RNA in Gene Regulation
    Gregory Hannon; Fiscal Year: 2005
    ..abstract_text> ..
  9. MECHANISMS OF DSRNA-INDUCED GENE SILENCING
    Gregory Hannon; Fiscal Year: 2003
    ..In this application, we outline our approach toward elucidating the mechanisms underlying dsRNA-induced gene silencing through a combination of in vitro and in vivo studies. ..
  10. MECHANISMS OF DSRNA-INDUCED GENE SILENCING
    Gregory Hannon; Fiscal Year: 2004
    ..In this application, we outline our approach toward elucidating the mechanisms underlying dsRNA-induced gene silencing through a combination of in vitro and in vivo studies. ..
  11. MECHANISMS OF DSRNA-INDUCED GENE SILENCING
    Gregory Hannon; Fiscal Year: 2003
    ..In this application, we outline our approach toward elucidating the mechanisms underlying dsRNA-induced gene silencing through a combination of in vitro and in vivo studies. ..
  12. MECHANISMS OF DSRNA-INDUCED GENE SILENCING
    Gregory Hannon; Fiscal Year: 2002
    ..In this application, we outline our approach toward elucidating the mechanisms underlying dsRNA-induced gene silencing through a combination of in vitro and in vivo studies. ..
  13. MECHANISMS OF DSRNA-INDUCED GENE SILENCING
    Gregory Hannon; Fiscal Year: 2002
    ..In this application, we outline our approach toward elucidating the mechanisms underlying dsRNA-induced gene silencing through a combination of in vitro and in vivo studies. ..
  14. Phenotype Arrays--An approach to Novel Anticancer Target
    Gregory Hannon; Fiscal Year: 2002
    ..The development of this technology will allow not only allow a search for anti-cancer targets but will also undoubtedly find application to a broad range of biological problems. ..
  15. MECHANISMS OF DSRNA-INDUCED GENE SILENCING
    Gregory J Hannon; Fiscal Year: 2010
    ..They serve as tools for understanding normal and aberrant biological processes and will perhaps transform clinical practice by serving as the basis for a new generation of therapeutic agents. ..