Bryan R Williams

Summary

Affiliation: Cleveland Clinic Foundation
Country: USA

Publications

  1. ncbi request reprint Immunology. A viral on/off switch for interferon
    Bryan R G Williams
    Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, OH 44195, USA
    Science 300:1100-1. 2003
  2. ncbi request reprint Signal integration via PKR
    B R Williams
    The author is in the Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Sci STKE 2001:re2. 2001
  3. ncbi request reprint RNA-dependent protein kinase PKR is required for activation of NF-kappa B by IFN-gamma in a STAT1-independent pathway
    A Deb
    Department of Cancer Biology, The Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    J Immunol 166:6170-80. 2001
  4. pmc Deficient cytokine signaling in mouse embryo fibroblasts with a targeted deletion in the PKR gene: role of IRF-1 and NF-kappaB
    A Kumar
    Department of Cancer Biology, The Cleveland Clinic Foundation, OH 44195, USA
    EMBO J 16:406-16. 1997
  5. pmc NF-kappaB activation by double-stranded-RNA-activated protein kinase (PKR) is mediated through NF-kappaB-inducing kinase and IkappaB kinase
    M Zamanian-Daryoush
    Department of Cancer Biology, The Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Mol Cell Biol 20:1278-90. 2000
  6. ncbi request reprint Negative regulation of CD8+ T cell function by the IFN-induced and double-stranded RNA-activated kinase PKR
    S Kadereit
    Departments of Cancer Biology and Immunology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    J Immunol 165:6896-901. 2000
  7. ncbi request reprint Interferon action in triply deficient mice reveals the existence of alternative antiviral pathways
    A Zhou
    The Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
    Virology 258:435-40. 1999
  8. pmc The protein kinase PKR is required for p38 MAPK activation and the innate immune response to bacterial endotoxin
    K C Goh
    Department of Cancer Biology NB40, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    EMBO J 19:4292-7. 2000
  9. pmc Interferon-alpha-induced phosphorylation and activation of cytosolic phospholipase A2 is required for the formation of interferon-stimulated gene factor three
    V Flati
    Department of Cancer Biology, Cleveland Clinic Foundation, OH 44195, USA
    EMBO J 15:1566-71. 1996
  10. pmc Receptor-associated constitutive protein tyrosine phosphatase activity controls the kinase function of JAK1
    S J Haque
    Department of Cancer Biology, Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Proc Natl Acad Sci U S A 94:8563-8. 1997

Collaborators

Detail Information

Publications63

  1. ncbi request reprint Immunology. A viral on/off switch for interferon
    Bryan R G Williams
    Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, OH 44195, USA
    Science 300:1100-1. 2003
  2. ncbi request reprint Signal integration via PKR
    B R Williams
    The author is in the Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Sci STKE 2001:re2. 2001
    ..Although the conflicts may not be resolved in this review, suggestions are made for experiments that could lead to a clearer understanding of the mechanisms involved...
  3. ncbi request reprint RNA-dependent protein kinase PKR is required for activation of NF-kappa B by IFN-gamma in a STAT1-independent pathway
    A Deb
    Department of Cancer Biology, The Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    J Immunol 166:6170-80. 2001
    ..There was no activation of NF-kappaB by IFN in PKR-null cells, indicating that PKR is required for IFN-gamma signaling to NF-kappaB...
  4. pmc Deficient cytokine signaling in mouse embryo fibroblasts with a targeted deletion in the PKR gene: role of IRF-1 and NF-kappaB
    A Kumar
    Department of Cancer Biology, The Cleveland Clinic Foundation, OH 44195, USA
    EMBO J 16:406-16. 1997
    ..Thus, PKR acts as a signal transducer for IFN-stimulated genes dependent on the transcription factors IRF-1 and NF-kappaB...
  5. pmc NF-kappaB activation by double-stranded-RNA-activated protein kinase (PKR) is mediated through NF-kappaB-inducing kinase and IkappaB kinase
    M Zamanian-Daryoush
    Department of Cancer Biology, The Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Mol Cell Biol 20:1278-90. 2000
    ..Taken together, these results demonstrate that PKR-dependent dsRNA induction of NF-kappaB is mediated by NIK and IKK activation...
  6. ncbi request reprint Negative regulation of CD8+ T cell function by the IFN-induced and double-stranded RNA-activated kinase PKR
    S Kadereit
    Departments of Cancer Biology and Immunology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    J Immunol 165:6896-901. 2000
    ..These results implicate PKR for the first time in the growth control of mature T lymphocytes and give insight into the negative regulation of CD8(+) T cell-mediated immune responses...
  7. ncbi request reprint Interferon action in triply deficient mice reveals the existence of alternative antiviral pathways
    A Zhou
    The Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
    Virology 258:435-40. 1999
    ..These results confirm the antiviral functions of RNase L and PKR in vivo but also provide unequivocal evidence for the existence of novel, innate immune pathways against viruses...
  8. pmc The protein kinase PKR is required for p38 MAPK activation and the innate immune response to bacterial endotoxin
    K C Goh
    Department of Cancer Biology NB40, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    EMBO J 19:4292-7. 2000
    ..Our findings indicate, for the first time, that PKR is required for p38 MAPK signaling and plays a potentially important role in the innate response against bacterial endotoxin...
  9. pmc Interferon-alpha-induced phosphorylation and activation of cytosolic phospholipase A2 is required for the formation of interferon-stimulated gene factor three
    V Flati
    Department of Cancer Biology, Cleveland Clinic Foundation, OH 44195, USA
    EMBO J 15:1566-71. 1996
    ..Thus, cPLA2 activations occurs as an early event in the IFN-alpha response and is selectively involved in ISGF3-dependent gene activation...
  10. pmc Receptor-associated constitutive protein tyrosine phosphatase activity controls the kinase function of JAK1
    S J Haque
    Department of Cancer Biology, Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Proc Natl Acad Sci U S A 94:8563-8. 1997
    ..Inhibition or deletion of PTP activity results in constitutive activation of cytokine signaling pathways...
  11. ncbi request reprint Two distinct tumor suppressor loci within chromosome 11p15 implicated in breast cancer progression and metastasis
    P Karnik
    Department of Cancer Biology, Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Hum Mol Genet 7:895-903. 1998
    ..The fine mapping of this intriguing chromosomal region should facilitate the cloning of the target genes and provide critical clues to understanding the mechanisms that contribute to the evolution of adult and childhood cancers...
  12. ncbi request reprint Protein-tyrosine phosphatase Shp-1 is a negative regulator of IL-4- and IL-13-dependent signal transduction
    S J Haque
    Department of Cancer Biology, The Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Biol Chem 273:33893-6. 1998
    ..These data clearly implicate Shp-1 in the negative regulation of the IL-4/IL-13-activated Jak-Stat pathway...
  13. ncbi request reprint Induction of E-selectin expression by double-stranded RNA and TNF-alpha is attenuated in murine aortic endothelial cells derived from double-stranded RNA-activated kinase (PKR)-null mice
    S K Bandyopadhyay
    Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    J Immunol 164:2077-83. 2000
    ....
  14. ncbi request reprint How cells respond to interferons
    G R Stark
    Lerner Research Institute, Cleveland Clinic Foundation, Ohio 44195, USA
    Annu Rev Biochem 67:227-64. 1998
    ....
  15. ncbi request reprint Identification of critical residues required for suppressor of cytokine signaling-specific regulation of interleukin-4 signaling
    S J Haque
    Department of Cancer Biology, Lerner Research Institute and the Department of Pulmonary and Critical Care Medicine, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Biol Chem 275:26500-6. 2000
    ..Thus, the Thr-Phe motif in the Pre-SH2 domain plays a critical role in SOCS-3-mediated inhibition of the IL-4-dependent Jak-Stat signaling, likely by regulating the mode of SOCS-Jak interaction...
  16. ncbi request reprint DRBP76, a double-stranded RNA-binding nuclear protein, is phosphorylated by the interferon-induced protein kinase, PKR
    R C Patel
    Department of Molecular Biology, Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Biol Chem 274:20432-7. 1999
    ..Finally, purified DRBP76 was shown to be a substrate of PKR in vitro, indicating that this protein's cellular activities may be regulated by PKR-mediated phosphorylation...
  17. ncbi request reprint In situ expression of the early growth response gene-1 during murine nephrogenesis
    R R Rackley
    Department of Urology, Cleveland Clinic Foundation, Ohio, USA
    J Urol 154:700-5. 1995
    ..This observation defines a spatial and temporal window during which WT1 competition for EGR-1 DNA binding sites may be involved in regulating EGR-1 expression...
  18. pmc Protein kinase PKR is required for platelet-derived growth factor signaling of c-fos gene expression via Erks and Stat3
    A Deb
    Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    EMBO J 20:2487-96. 2001
    ..Taken together, these results establish PKR as an upstream regulator of activation of Stat3 and as a common mediator of both growth-promoting and growth-inhibitory signals...
  19. ncbi request reprint HIV-I TAT inhibits PKR activity by both RNA-dependent and RNA-independent mechanisms
    R Cai
    Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
    Arch Biochem Biophys 373:361-7. 2000
    ....
  20. ncbi request reprint Genomic features of human PKR: alternative splicing and a polymorphic CGG repeat in the 5'-untranslated region
    Z Xu
    Department of Genetics, Case Western Reserve University, Lerner Research Institute, Cleveland Clinic Foundation, OH 44106, USA
    J Interferon Cytokine Res 18:609-16. 1998
    ..Although the human and murine PKR proteins have high homology, comparison of their gene structures reveals divergence in 5'-flanking regions, suggesting distinct regulation at the genomic level...
  21. ncbi request reprint Functional classification of interferon-stimulated genes identified using microarrays
    M J de Veer
    Department of Cancer Biology, Lerner Research Institute, Cleveland, Ohio, USA
    J Leukoc Biol 69:912-20. 2001
    ....
  22. pmc Regulation of WT1 by phosphorylation: inhibition of DNA binding, alteration of transcriptional activity and cellular translocation
    Y Ye
    Department of Cancer Biology, Cleveland Clinic Foundation, OH 44195 5001, USA
    EMBO J 15:5606-15. 1996
    ..Accordingly, phosphorylation may play a role in modulating the transcriptional regulatory activity of WT1 through interference with nuclear translocation, as well as by inhibition of WT1 DNA binding...
  23. pmc The B56alpha regulatory subunit of protein phosphatase 2A is a target for regulation by double-stranded RNA-dependent protein kinase PKR
    Z Xu
    Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Mol Cell Biol 20:5285-99. 2000
    ..Taken together, our data indicate that PKR can modulate PP2A activity by phosphorylating B56alpha to regulate cellular activities...
  24. ncbi request reprint PKR; a sentinel kinase for cellular stress
    B R Williams
    Department of Cancer Biology NB40, Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio, OH 44195, USA
    Oncogene 18:6112-20. 1999
    ....
  25. ncbi request reprint HIV-1 Tat directly interacts with the interferon-induced, double-stranded RNA-dependent kinase, PKR
    N A McMillan
    Department of Cancer Biology, Cleveland Clinic Foundation, Ohio 44195, USA
    Virology 213:413-24. 1995
    ..The interaction of Tat with PKR provides a potential mechanism by which HIV could suppress the interferon system...
  26. pmc Structure of the double-stranded RNA-binding domain of the protein kinase PKR reveals the molecular basis of its dsRNA-mediated activation
    S Nanduri
    Structural Biology Program, Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    EMBO J 17:5458-65. 1998
    ..The central linker is highly flexible, which may enable the two dsRBMs to wrap around the RNA duplex for cooperative and high-affinity binding, leading to the overall change of PKR conformation and its activation...
  27. ncbi request reprint Antisense transcripts and protein binding motifs within the Wilms tumour (WT1) locus
    C E Campbell
    Department of Cancer Biology, Cleveland Clinic Foundation, Ohio 44195
    Oncogene 9:583-95. 1994
    ..Interestingly, recombinant WT1, representing the product of zinc finger region of the most abundant of the four alternatively spliced transcripts, is also capable of binding to sequences within this region...
  28. doi request reprint Interferon-stimulated genes and their protein products: what and how?
    Ernest C Borden
    Cleveland Clinic and Case Comprehensive Cancer Center, Taussig Cancer Institute, Cleveland, Ohio 44195, USA
    J Interferon Cytokine Res 31:1-4. 2011
    ....
  29. ncbi request reprint Apoptosis and interferons: role of interferon-stimulated genes as mediators of apoptosis
    M Chawla-Sarkar
    Center for Drug Discovery and Development, Taussig Cancer Center, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Apoptosis 8:237-49. 2003
    ..In vitro IFN-alpha, IFN-beta and IFN-gamma induced apoptosis in multiple cell lines of varied histologies. This review will emphasize possible mechanisms and the role of ISGs involved in mediating apoptotic function of IFNs...
  30. pmc Expression of the Von Hippel-Lindau tumor suppressor gene, VHL, in human fetal kidney and during mouse embryogenesis
    P M Kessler
    Department of Cancer Biology, Cleveland Clinic Foundation, Ohio 44195, USA
    Mol Med 1:457-66. 1995
    ..Not only are mutations in this gene responsible for the VHL syndrome, but mutations are also very frequent in sporadic RCC...
  31. ncbi request reprint Characterization of the solution complex between the interferon-induced, double-stranded RNA-activated protein kinase and HIV-I trans-activating region RNA
    B W Carpick
    Department of Cancer Biology, Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Biol Chem 272:9510-6. 1997
    ..Taken together, these data indicate that the PKR activation complex consists of a protein dimer bound cooperatively to one dsRNA molecule...
  32. ncbi request reprint Identification and characterization of human beta 2-chimaerin: association with malignant transformation in astrocytoma
    S Yuan
    Brain Tumor Center of the Department of Neurological Surgery, Cleveland Clinic Foundation, Ohio 44195, USA
    Cancer Res 55:3456-61. 1995
    ..The down-regulation of beta 2-chimaerin expression in the high-grade gliomas suggests that decreased expression of this gene may be a feature of progression in the development of malignant glioma...
  33. pmc Analysis of genes induced by Sendai virus infection of mutant cell lines reveals essential roles of interferon regulatory factor 3, NF-kappaB, and interferon but not toll-like receptor 3
    Christopher P Elco
    Department of Molecular Biology, Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Ave, Cleveland, OH 44195, USA
    J Virol 79:3920-9. 2005
    ..Thus, IRF-3 can suppress the expression of NF-kappaB-dependent genes in SeV-infected cells...
  34. ncbi request reprint Detection of foreign RNA: implications for RNAi
    Michael J de Veer
    Centre for Animal Biotechnology, The University of Melbourne, Victoria, Australia
    Immunol Cell Biol 83:224-8. 2005
    ..With the recent advances in RNAi technology and its first forays into the in vivo setting now coming to light, it is pertinent to review the cellular response to ribonucleic acids typically used in RNAi methods...
  35. ncbi request reprint TLR4, but not TLR2, mediates IFN-beta-induced STAT1alpha/beta-dependent gene expression in macrophages
    Vladimir Toshchakov
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Nat Immunol 3:392-8. 2002
    ..Together, these findings provide the first mechanistic basis for differential patterns of gene expression activated by TLR4 and TLR2 agonists...
  36. pmc Phospholipid scramblase 1 potentiates the antiviral activity of interferon
    Beihua Dong
    Department of Cancer Biology, The Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Virol 78:8983-93. 2004
    ..Our results suggest that PLSCR1, which is itself an ISG-encoded protein, provides a mechanism for amplifying and enhancing the IFN response through increased expression of a select subset of potent antiviral genes...
  37. pmc Induction of interferon-stimulated gene expression and antiviral responses require protein deacetylase activity
    Hao Ming Chang
    Department of Pathology and NYU Cancer Institute, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
    Proc Natl Acad Sci U S A 101:9578-83. 2004
    ..Requirement for HDAC function in transcriptional activation may represent a general mechanism for rapid stimulation of ISG transcription...
  38. pmc Interferon-inducible antiviral effectors
    Anthony J Sadler
    Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, Australia
    Nat Rev Immunol 8:559-68. 2008
    ..Ongoing research continues to expose additional activities for these effector proteins and has revealed unanticipated functions of the antiviral response...
  39. ncbi request reprint Double-stranded RNA-dependent protein kinase (PKR) is downregulated by phorbol ester
    Yan Zhou
    Department of Chemistry, Cleveland State University, OH 44115, USA
    FEBS J 272:1568-76. 2005
    ..Furthermore, we also found that proteasome inhibitors prevented PMA-induced down regulation of PKR, indicating that an active proteasome is required. Our results identify a novel mechanism for the post-translational regulation of PKR...
  40. ncbi request reprint Stability of CXCL-8 and related AU-rich mRNAs in the context of hepatitis C virus replication in vitro
    Jamison Green
    Department of Laboratory Medicine, University of Washington, Seattle, 98104 2499, USA
    J Infect Dis 193:802-11. 2006
    ..CXCL-8 mRNA stability was, therefore, investigated in the context of HCV replication in 4 replicon cell lines, Huh7 and Huh7.5 cells, and primary human fetal hepatocytes...
  41. ncbi request reprint Light controllable siRNAs regulate gene suppression and phenotypes in cells
    Quan N Nguyen
    Genospectra, Inc, 6519 Dumbarton Circle, Fremont, CA 94555, USA
    Biochim Biophys Acta 1758:394-403. 2006
    ..The csiRNAs are comparable to standard siRNAs in their transfection efficiency and potency of gene silencing. This technology should be of interest for phenotypic assays such as cell survival, cell cycle regulation, and cell development...
  42. ncbi request reprint A structural basis for discriminating between self and nonself double-stranded RNAs in mammalian cells
    Joao Trindade Marques
    Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Nat Biotechnol 24:559-65. 2006
    ..These findings will enable the rational design of siRNAs that avoid nonspecific effects or, alternatively, that induce bystander effects to potentially increase the efficacy of siRNA-based treatments of viral infections or cancer...
  43. ncbi request reprint Dynamic flexibility of double-stranded RNA activated PKR in solution
    Frank Gabel
    Structural and Computational Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    J Mol Biol 359:610-23. 2006
    ..The results of this study emphasize the usefulness of low-resolution structural studies in solution on large flexible multiple domain proteins...
  44. pmc Replication of hepatitis C virus (HCV) RNA in mouse embryonic fibroblasts: protein kinase R (PKR)-dependent and PKR-independent mechanisms for controlling HCV RNA replication and mediating interferon activities
    Kyung Soo Chang
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, 800 Rose Street, Lexington, KY 40536 0298, USA
    J Virol 80:7364-74. 2006
    ....
  45. ncbi request reprint Dicing with siRNA
    Bryan R G Williams
    Nat Biotechnol 23:181-2. 2005
  46. pmc Protein kinase R-dependent regulation of interleukin-10 in response to double-stranded RNA
    Arindam Chakrabarti
    Departments of Cancer Biology and Molecular Cardiology, Lerner Research Institute, The Cleveland Clinic, Cleveland, OH 44195, USA
    J Biol Chem 283:25132-9. 2008
    ..Together, our data demonstrate the role of PKR in regulating an anti-inflammatory cytokine. The findings have significance in antiviral as well as broader innate immune responses...
  47. ncbi request reprint Salicylates trigger protein synthesis inhibition in a protein kinase R-like endoplasmic reticulum kinase-dependent manner
    Aristobolo M Silva
    Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Biol Chem 282:10164-71. 2007
    ..Thus, salicylates selectively trigger an endoplasmic reticulum stress-responsive signaling pathway initiated through activation of PERK to induce their cellular effects...
  48. pmc Tissue-specific and inducer-specific differential induction of ISG56 and ISG54 in mice
    Fulvia Terenzi
    Department of Molecular Genetics NE20, The Lewrnert research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    J Virol 81:8656-65. 2007
    ..Furthermore, our findings provide the first evidence of a differential pattern of expression of ISG54 and ISG56 genes by IFN-alpha and IFN-beta...
  49. ncbi request reprint Cystic fibrosis and normal human airway epithelial cell response to influenza a viral infection
    Weiling Xu
    Department of Pathobiology and Pulmonary Allergy and Critical Care Medicine, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    J Interferon Cytokine Res 26:609-27. 2006
    ..Taken together, the lesser antiviral and greater early inflammatory response likely contribute to the severe respiratory illness of CF patients with viral infections...
  50. pmc Involvement of the interferon-regulated antiviral proteins PKR and RNase L in reovirus-induced shutoff of cellular translation
    Jennifer A Smith
    Department of Microbiology, University of Minnesota, 420 Delaware St, Minneapolis, MN 55455, USA
    J Virol 79:2240-50. 2005
    ..In addition, our results suggest that reovirus has evolved effective mechanisms to avoid the actions of the interferon-stimulated antiviral pathways that include PKR and RNase L and may even benefit from their expression...
  51. pmc Blockade of interferon induction and action by the E3L double-stranded RNA binding proteins of vaccinia virus
    Ying Xiang
    Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    J Virol 76:5251-9. 2002
    ..Furthermore, our results indicate the existence of an interferon-mediated antipoxvirus pathway that operates independently of PKR, Mx1, or the 2-5A/RNase L system...
  52. ncbi request reprint RNase L mediates transient control of the interferon response through modulation of the double-stranded RNA-dependent protein kinase PKR
    Khalid S A Khabar
    Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Center, Riyadh 11211, Saudi Arabia
    J Biol Chem 278:20124-32. 2003
    ..These results suggest a role for RNase L in the transient control of the IFN response and possibly of other cytokine and stress responses...
  53. ncbi request reprint Type I interferon induction pathway, but not released interferon, participates in the maturation of dendritic cells induced by negative-strand RNA viruses
    CAROLINA B LOPEZ
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    J Infect Dis 187:1126-36. 2003
    ..It was demonstrated that these pathways have different sensitivities to the presence of viral stimulus...
  54. ncbi request reprint C114 is a novel IL-11-inducible nuclear double-stranded RNA-binding protein that inhibits protein kinase R
    Zhan Yin
    Department of Pharmacology and Cancer Center, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4965, USA
    J Biol Chem 278:22838-45. 2003
    ..C) treatment. This is the first demonstration of a novel PKR modulator induced by a gp130 superfamily cytokine that may play a role in cytokine-mediated biological functions...
  55. ncbi request reprint Impaired innate host defense causes susceptibility to respiratory virus infections in cystic fibrosis
    Shuo Zheng
    Department of Pulmonary and Critical Care Medicine, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Immunity 18:619-30. 2003
    ..NO donor or NOS2 overexpression provides protection from virus infection in CF, suggesting that NO is sufficient for antiviral host defense in the human airway and is one strategy for antiviral therapy in CF children...
  56. ncbi request reprint TLR2 and TLR4 agonists stimulate unique repertoires of host resistance genes in murine macrophages: interferon-beta-dependent signaling in TLR4-mediated responses
    Vladimir Toshchakov
    Department of Microbiology and Immunology, University of Maryland, 655 W Baltimore Street, Baltimore, MD 21201, USA
    J Endotoxin Res 9:169-75. 2003
    ....
  57. ncbi request reprint Activation of the interferon system by short-interfering RNAs
    Carol A Sledz
    Department of Cancer Biology, Lerner Research Institute, NB40, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195 USA
    Nat Cell Biol 5:834-9. 2003
    ..Thus, siRNAs have broad and complicating effects beyond the selective silencing of target genes when introduced into cells. This is of critical importance, as siRNAs are currently being explored for their potential therapeutic use...
  58. ncbi request reprint Limited role of N-terminal phosphoserine residues in the activation of transcription by p53
    Mark W Jackson
    Department of Molecular Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Oncogene 23:4477-87. 2004
    ....
  59. ncbi request reprint The Wilms tumor suppressor-1 target gene podocalyxin is transcriptionally repressed by p53
    Patricia Stanhope-Baker
    Departments of Cancer Biology and Molecular Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Biol Chem 279:33575-85. 2004
    ..We propose that inappropriate expression of PODXL due to changes in WT1 and/or p53 activity may contribute to Wilms tumorigenesis...
  60. pmc PKR-dependent and -independent mechanisms are involved in translational shutoff during Sindbis virus infection
    Rodion Gorchakov
    Department of Microbiology and Immunology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555 1019, USA
    J Virol 78:8455-67. 2004
    ..Specifically, we show that expression of heterologous genes from SIN-based and most likely other alphavirus-based replicons can be increased by downregulating both the PKR-dependent and PKR-independent translational shutoffs...
  61. ncbi request reprint Synergistic activation of innate immunity by double-stranded RNA and CpG DNA promotes enhanced antitumor activity
    Mark M Whitmore
    Department of Cancer Biology, Taussig Cancer Center, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Cancer Res 64:5850-60. 2004
    ..e., dsRNA and CpG DNA, may mimic definitive viral recognition, resulting in an enhanced innate immune response that could be used for tumor vaccination or immunotherapy...
  62. ncbi request reprint Biochemical analyses of multiple fractions of PKR purified from Escherichia coli
    Zan Xu
    Department of Cancer Biology, NB40, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    J Interferon Cytokine Res 24:522-35. 2004
    ..The production of multiple fractions of PKR with different biochemical properties in E. coli suggests that the spectrum of PKR activity and regulation in mammalian cells is likely to be similarly complex...
  63. pmc Interferon-regulated pathways that control hepatitis B virus replication in transgenic mice
    Luca G Guidotti
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Virol 76:2617-21. 2002
    ..Finally, RNase L(-/-) transgenic mice were undistinguishable from controls under basal conditions and after poly(I-C) or IFN-gamma administration, suggesting that RNase L does not modulate HBV replication in this model...