Dennis Stacey

Summary

Affiliation: Cleveland Clinic Foundation
Country: USA

Publications

  1. pmc Glycogen synthase kinase 3 has a limited role in cell cycle regulation of cyclin D1 levels
    Ke Yang
    Department of Molecular Genetics, The Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland OH, USA
    BMC Cell Biol 7:33. 2006
  2. ncbi request reprint Cyclin D1 serves as a cell cycle regulatory switch in actively proliferating cells
    Dennis W Stacey
    Department of Molecular Biology, The Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Curr Opin Cell Biol 15:158-63. 2003
  3. doi request reprint Cell cycle studies based upon quantitative image analysis
    Dennis W Stacey
    Department of Molecular Genetics, The Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Cytometry A 73:270-8. 2008
  4. pmc Influence of cell cycle and oncogene activity upon topoisomerase IIalpha expression and drug toxicity
    D W Stacey
    Department of Molecular Biology, The Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Mol Cell Biol 20:9127-37. 2000
  5. ncbi request reprint Prostaglandin A2 blocks the activation of G1 phase cyclin-dependent kinase without altering mitogen-activated protein kinase stimulation
    M Hitomi
    Department of Molecular Biology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Biol Chem 271:9376-83. 1996
  6. ncbi request reprint Protection against cyclophosphamide-induced alopecia and inhibition of mammary tumor growth by topical 1,25-dihydroxyvitamin D3 in mice
    G Chen
    Department of Molecular Biology, The Cleveland Clinic Foundation, OH 44195, USA
    Int J Cancer 75:303-9. 1998
  7. ncbi request reprint Ras-dependent cell cycle commitment during G2 phase
    M Hitomi
    Department of Molecular Biology, The Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    FEBS Lett 490:123-31. 2001
  8. ncbi request reprint The p38 pathway provides negative feedback for Ras proliferative signaling
    G Chen
    Cleveland Clinic Foundation, Department of Molecular Biology, Cleveland, Ohio 44195, USA
    J Biol Chem 275:38973-80. 2000
  9. ncbi request reprint Regulation of Ras signaling by the cell cycle
    Dennis Stacey
    Department of Molecular Biology, The Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
    Curr Opin Genet Dev 12:44-6. 2002
  10. ncbi request reprint p27Kip1 and cyclin dependent kinase 2 regulate passage through the restriction point
    Masahiro Hitomi
    The Department of Molecular Genetics, The Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Cell Cycle 5:2281-9. 2006

Research Grants

  1. Control of topoisomerose II through the cell cycle
    Dennis Stacey; Fiscal Year: 2004
  2. FUNCTION OF RAS ACTIVITY LATE IN THE CELL CYCLE
    Dennis Stacey; Fiscal Year: 2002
  3. FUNCTION OF RAS ACTIVITY LATE IN THE CELL CYCLE
    Dennis Stacey; Fiscal Year: 2006

Collaborators

  • G Chen
  • M Hitomi
  • Jiali Han
  • Suk Paul Oh
  • Yang Guo
  • Gaurisankar Sa
  • Jyoti Harwalkar
  • Ke Yang
  • William C Stacey
  • Jonathan Fretthold
  • Jeffrey M Nye
  • Michelle D Garrett
  • David R Mason
  • Guan Chen GC
  • Andrew W Stacey

Detail Information

Publications17

  1. pmc Glycogen synthase kinase 3 has a limited role in cell cycle regulation of cyclin D1 levels
    Ke Yang
    Department of Molecular Genetics, The Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland OH, USA
    BMC Cell Biol 7:33. 2006
    ....
  2. ncbi request reprint Cyclin D1 serves as a cell cycle regulatory switch in actively proliferating cells
    Dennis W Stacey
    Department of Molecular Biology, The Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Curr Opin Cell Biol 15:158-63. 2003
    ..In this way, cyclin D1 is proposed to serve as an active switch in the regulation of continued cell cycle progression...
  3. doi request reprint Cell cycle studies based upon quantitative image analysis
    Dennis W Stacey
    Department of Molecular Genetics, The Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Cytometry A 73:270-8. 2008
    ..This model explains how cell cycle progression is terminated, and how the length of the cell cycle is regulated...
  4. pmc Influence of cell cycle and oncogene activity upon topoisomerase IIalpha expression and drug toxicity
    D W Stacey
    Department of Molecular Biology, The Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Mol Cell Biol 20:9127-37. 2000
    ..We conclude that both cell cycle phase and oncogenic signaling influence drug toxicity independently of alterations in topo IIalpha levels...
  5. ncbi request reprint Prostaglandin A2 blocks the activation of G1 phase cyclin-dependent kinase without altering mitogen-activated protein kinase stimulation
    M Hitomi
    Department of Molecular Biology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Biol Chem 271:9376-83. 1996
    ..PGA2 treatment elevated the p21Waf1/Cip1/Sdi1 protein expression level. These data indicate that PGA2 may arrest the cell cycle in G1 by interfering with the activation of G1 phase CDKs...
  6. ncbi request reprint Protection against cyclophosphamide-induced alopecia and inhibition of mammary tumor growth by topical 1,25-dihydroxyvitamin D3 in mice
    G Chen
    Department of Molecular Biology, The Cleveland Clinic Foundation, OH 44195, USA
    Int J Cancer 75:303-9. 1998
    ..Moreover, 1,25(OH)2D3 was shown, either alone or in combination with CTX, to inhibit tumor growth...
  7. ncbi request reprint Ras-dependent cell cycle commitment during G2 phase
    M Hitomi
    Department of Molecular Biology, The Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    FEBS Lett 490:123-31. 2001
    ..Thus, Ras activity during G2 phase induces cyclin D1 expression. This expression continues through the next G1 phase even in the absence of Ras activity, and drives cells into S phase...
  8. ncbi request reprint The p38 pathway provides negative feedback for Ras proliferative signaling
    G Chen
    Cleveland Clinic Foundation, Department of Molecular Biology, Cleveland, Ohio 44195, USA
    J Biol Chem 275:38973-80. 2000
    ..These studies thus establish a negative feedback mechanism by which Ras proliferative activity is regulated via signaling integrations of MAPK pathways...
  9. ncbi request reprint Regulation of Ras signaling by the cell cycle
    Dennis Stacey
    Department of Molecular Biology, The Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
    Curr Opin Genet Dev 12:44-6. 2002
    ..It is only during the later stages of G(1) phase that PI3K activity promotes entry into S-phase. In cycling cells, Ras activity is enhanced throughout the cell cycle, but is able to stimulate cyclin D1 elevation only during G(2) phase...
  10. ncbi request reprint p27Kip1 and cyclin dependent kinase 2 regulate passage through the restriction point
    Masahiro Hitomi
    The Department of Molecular Genetics, The Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Cell Cycle 5:2281-9. 2006
    ..We conclude that the p27 expression level at mid-G(1) phase determines when a cell passes through the restriction point, and does so by regulating subsequent CDK2 activation...
  11. ncbi request reprint The regulation of S phase initiation by p27Kip1 in NIH3T3 cells
    Gaurisankar Sa
    Department of Molecular Genetics, The Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Cell Cycle 4:618-27. 2005
    ..We propose that p27 directly regulates the initiation of DNA synthesis in NIH3T3 cells, and that cyclin D1 serves to modulate this activity...
  12. ncbi request reprint Phosphorylation of cyclin D1 at Thr 286 during S phase leads to its proteasomal degradation and allows efficient DNA synthesis
    Yang Guo
    Department of Molecular Genetics, The Lerner Research Institute, NC2 151, The Cleveland Clinic Foundation, 9500 Euclid Ave, Cleveland, OH 44195, USA
    Oncogene 24:2599-612. 2005
    ..In such cells, however, the levels of cyclin D1 would presumably be too high to be suppressed during S phase, resulting in the inhibition of DNA synthesis...
  13. ncbi request reprint Destabilization of cyclin D1 message plays a critical role in cell cycle exit upon mitogen withdrawal
    Yang Guo
    The Department of Molecular Biology, The Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Oncogene 24:1032-42. 2005
    ..These results demonstrate that cyclin D1 message destabilization plays a critical role in cyclin D1 suppression during G2 phase of serum-deprived cultures, and therefore in the withdrawal from the cell cycle...
  14. ncbi request reprint P27 expression is regulated by separate signaling pathways, downstream of Ras, in each cell cycle phase
    Gaurisankar Sa
    Department of Molecular Biology, The Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Exp Cell Res 300:427-39. 2004
    ..The fact that separate signaling pathways downstream of Ras function in each cell cycle phase to suppress p27 levels helps explain the vital connection between proliferative signaling, cell cycle control, and p27 expression...
  15. ncbi request reprint Ras is active throughout the cell cycle, but is able to induce cyclin D1 only during G2 phase
    Gaurisankar Sa
    The Department of Molecular Biology, The Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Ave, Ohio 44195, USA
    Cell Cycle 1:50-8. 2002
    ..We conclude that in the continuously cycling cell the targets of Ras activity are controlled by cell cycle phase, and that this phenomenon is vital to cell cycle progression...
  16. pmc Phosphorylation of cyclin D1 regulated by ATM or ATR controls cell cycle progression
    Masahiro Hitomi
    Department of Molecular Genetics, The Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Mol Cell Biol 28:5478-93. 2008
    ..These results indicate that checkpoint kinases play a critical role in regulating cell cycle progression in normal and stressed cells by directing the phosphorylation of cyclin D1...
  17. ncbi request reprint Post-transcriptional regulation of cyclin D1 expression during G2 phase
    Yang Guo
    The Department of Molecular Biology, NC2 150 The Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio, OH 44195, USA
    Oncogene 21:7545-56. 2002
    ..These data indicate a fundamental difference in the regulation of cyclin D1 production during continuous cell cycle progression and re-initiation of the cell cycle...

Research Grants16

  1. Control of topoisomerose II through the cell cycle
    Dennis Stacey; Fiscal Year: 2004
    ..Perhaps our studies of these slowly cycling cells will yield information of value in understanding these temporarily non-cycling tumor cells and their sensitivity to drug treatment. ..
  2. FUNCTION OF RAS ACTIVITY LATE IN THE CELL CYCLE
    Dennis Stacey; Fiscal Year: 2002
    ..These studies have the potential to broaden our understanding of the role of ras activity throughout the cell cycle in the control of cellular proliferation. ..
  3. FUNCTION OF RAS ACTIVITY LATE IN THE CELL CYCLE
    Dennis Stacey; Fiscal Year: 2006
    ..Studies of related molecules will also be performed. Eventually, it is our goal to understand in detail the role of G2 events in the control of cell growth. ..