Research Topics
| Robert SilvermanSummaryAffiliation: Cleveland Clinic Foundation Country: USA Publications
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Detail Information
Publications
Characterization of antibodies elicited by XMRV infection and development of immunoassays useful for epidemiologic studiesXiaoxing Qiu
Infectious Diseases R and D, Abbott Diagnostics, 100 Abbott Park Rd, Abbott Park, IL 60064, USA
Retrovirology 7:68. 2010..Development of assays to detect XMRV-specific antibodies would greatly facilitate such studies. However, the nature and kinetics of the antibody response to XMRV infection have yet to be determined...- The xenotropic murine leukemia virus-related retrovirus debate continues at first international workshopJonathan P Stoye
MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW71AA, UK
Retrovirology 7:113. 2010..The current status of XMRV research, concerns among the scientific community and suggestions for future actions are summarized in this meeting report... - Viral encounters with 2',5'-oligoadenylate synthetase and RNase L during the interferon antiviral responseRobert H Silverman
Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue NB40, Cleveland, OH 44195, USA
J Virol 81:12720-9. 2007 - A scientific journey through the 2-5A/RNase L systemRobert H Silverman
Department of Cancer Biology, NB40 Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA
Cytokine Growth Factor Rev 18:381-8. 2007..My scientific journey through the 2-5A system recounts some of the highlights of these efforts. Knowledge gained from studies on the 2-5A system could have an impact on development of therapies for important viral pathogens and cancer... 
Suppression of ovarian carcinoma cell growth in vivo by the interferon-inducible plasma membrane protein, phospholipid scramblase 1Robert H Silverman
Department of Cancer Biology, Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
Cancer Res 62:397-402. 2002..These data suggest that the up-regulation of PLSCR1 expression in tumor cells exposed to IFN may contribute to the observed tumor-suppressive action of this cytokine...- Skin allograft rejection is suppressed in mice lacking the antiviral enzyme, 2',5'-oligoadenylate-dependent RNase LRobert H Silverman
Department of Cancer Biology, Lerner Research Institute, The Cleveland Clinic Foundation, Ohio 44195, USA
Viral Immunol 15:77-83. 2002..Results consistent with a proinflammatory role for RNase L extend the known functions of the 2-5A/RNase L system beyond innate immunity into some, but not all, types of cellular immunity... 
The human retrovirus XMRV in prostate cancer and chronic fatigue syndromeRobert H Silverman
Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA
Nat Rev Urol 7:392-402. 2010..Emerging science surrounding XMRV is contributing to our knowledge of retroviral infections while focusing intense interest on two major human diseases...- Implications for RNase L in prostate cancer biologyRobert H Silverman
Department of Cancer Biology, Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
Biochemistry 42:1805-12. 2003..This monograph reviews the biochemistry and genetics of RNase L as it relates to the pathobiology of prostate cancer and considers implications for future screening and therapy of this disease... - Phospholipid scramblase 1 potentiates the antiviral activity of interferonBeihua Dong
Department of Cancer Biology, The Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
J Virol 78:8983-93. 2004..Our results suggest that PLSCR1, which is itself an ISG-encoded protein, provides a mechanism for amplifying and enhancing the IFN response through increased expression of a select subset of potent antiviral genes... - Pathological aggressiveness of prostatic carcinomas related to RNASEL R462Q allelic variantsBenjamin T Larson
Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland Clinic, Cleveland, Ohio, USA
J Urol 179:1344-8. 2008..We assessed a series of men undergoing radical prostatectomy for clinical and pathological measures of tumor aggressiveness according to the RNASEL R462Q genotype... - Interferons at age 50: past, current and future impact on biomedicineErnest C Borden
Taussig Cancer Center, Case Comprehensive Cancer Center, Mellen Center for Multiple Sclerosis, and Lerner Research Institute, The Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
Nat Rev Drug Discov 6:975-90. 2007..Our goal is to offer a molecular and clinical perspective that will enable IFNs or their TLR agonist inducers to reach their full clinical potential... - An apoptotic signaling pathway in the interferon antiviral response mediated by RNase L and c-Jun NH2-terminal kinaseGeqiang Li
Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
J Biol Chem 279:1123-31. 2004..These findings suggest that JNK and RNase L function in an integrated signaling pathway during the IFN response that leads to elimination of virus-infected cells through apoptosis... - RNASEL Arg462Gln variant is implicated in up to 13% of prostate cancer casesGraham Casey
Department of Cancer Biology, ND50, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
Nat Genet 32:581-3. 2002..Men that are heterozygous with respect to the mutated allele have 50% greater risk of prostate cancer than non-carriers, and homozygotes have more than double the risk... - Inflammation, infection, and prostate cancerEric A Klein
Glickman Urologic and Kidney Institute, Cleveland, Ohio, USA
Curr Opin Urol 18:315-9. 2008..Recent evidence suggesting that inflammation and infection play a role in the etiology of prostate cancer, and the discovery of a novel virus in men with a genetic susceptibility to prostate cancer is reviewed... - Tumour suppressor function of RNase L in a mouse modelWendy Liu
Department of Chemistry, Clinical Chemistry Program, Cleveland State University, 2121 Euclid Avenue, Cleveland, OH 44115, USA
Eur J Cancer 43:202-9. 2007..Further analysis revealed, however, that these tumours no longer expressed ectopic RNase L. Our findings suggest that RNase L plays a critical role in the inhibition of fibrosarcoma growth in nude mice... - A transcriptional signaling pathway in the IFN system mediated by 2'-5'-oligoadenylate activation of RNase LKrishnamurthy Malathi
Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
Proc Natl Acad Sci U S A 102:14533-8. 2005..Because 2-5A and RNase L participate in defenses against viral infections and prostate cancer, our findings have implications for basic cellular mechanisms that control major pathogenic processes... - Genetic susceptibility and oxidative stress in prostate cancer: integrated model with implications for preventionEric A Klein
Section of Urologic Oncology, Glickman Urological Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44122, USA
Urology 68:1145-51. 2006 - Small-molecule activators of RNase L with broad-spectrum antiviral activityChandar S Thakur
Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA
Proc Natl Acad Sci U S A 104:9585-90. 2007..Therefore, these RNase L activators are prototypes for a previously uncharacterized class of broad-spectrum antiviral agents... - 2-5A ligands--a new concept for the treatment of prostate cancerHagen Cramer
Ridgeway Biosystems Inc, Cleveland, Ohio, USA
Nucleosides Nucleotides Nucleic Acids 26:1471-7. 2007..78 nM) as a folate hydrolase inhibitor, while SMCC 2-5A lacking the ZJ-24 part, did not show much activity (IC50 = 1974 nM). Also, RBI 1033's affinity to PSMA was found to be 10 times higher than ZJ-24 itself... - Blockade of interferon induction and action by the E3L double-stranded RNA binding proteins of vaccinia virusYing Xiang
Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
J Virol 76:5251-9. 2002..Furthermore, our results indicate the existence of an interferon-mediated antipoxvirus pathway that operates independently of PKR, Mx1, or the 2-5A/RNase L system... - An infectious retrovirus susceptible to an IFN antiviral pathway from human prostate tumorsBeihua Dong
Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA
Proc Natl Acad Sci U S A 104:1655-60. 2007..Our studies demonstrate that XMRV is a virus that has infected humans and is susceptible to inhibition by IFN and its downstream effector, RNase L... - HPC1/RNASEL mediates apoptosis of prostate cancer cells treated with 2',5'-oligoadenylates, topoisomerase I inhibitors, and tumor necrosis factor-related apoptosis-inducing ligandKrishnamurthy Malathi
Department of Cancer Biology, Lerner Research Institute, and Experimental Therapeutics Program, Taussig Cancer Center, The Cleveland Clinic Foundation, Cleveland, Ohio, USA
Cancer Res 64:9144-51. 2004..These findings indicate that RNase L integrates and amplifies apoptotic signals generated during treatment of prostate cancer cells with 2-5A, Topo I inhibitors, and TRAIL... - RNase L releases a small RNA from HCV RNA that refolds into a potent PAMPKrishnamurthy Malathi
Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
RNA 16:2108-19. 2010..It is proposed that RNase L mediated cleavage of HCV RNA generates svRNA that activates RIG-I, thus propagating innate immune signaling to the IFN-β gene... - RNase L mediated protection from virus induced demyelinationDerek D C Ireland
Department of Neuroscience, Lerner Research Institute, The Cleveland Clinic, Cleveland, Ohio, United States of America
PLoS Pathog 5:e1000602. 2009..Protective function is rather associated with cell type specific and regional restriction of viral replication in grey matter and ameliorated neurodegeneration and demyelination... - Targeted therapy of respiratory syncytial virus in African green monkeys by intranasally administered 2-5A antisenseDouglas W Leaman
Ridgeway Biosystems Inc, 9500 Euclid Avenue, NE50, Cleveland, Ohio 44195, USA
Virology 292:70-7. 2002..When administered intranasally to RSV-infected African green monkeys, this chimera reduced nasal RSV replication by up to four log(10) units in a dose- and time-dependent manner... - Fibrils of prostatic acid phosphatase fragments boost infections with XMRV (xenotropic murine leukemia virus-related virus), a human retrovirus associated with prostate cancerSeunghee Hong
Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
J Virol 83:6995-7003. 2009.... - Small self-RNA generated by RNase L amplifies antiviral innate immunityKrishnamurthy Malathi
Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
Nature 448:816-9. 2007..Our results indicate that RNase L has an essential role in the innate antiviral immune response that relieves the requirement for direct sensing of non-self RNA... - Inhibition of human parainfluenza virus type 3 infection by novel small moleculesHongxia Mao
Department of Molecular Genetics, Virology Section NN10, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
Antiviral Res 77:83-94. 2008..Use of these inhibitors has a strong potential to develop novel antiviral agents against this important human pathogen... - Selection and cloning of poly(rC)-binding protein 2 and Raf kinase inhibitor protein RNA activators of 2',5'-oligoadenylate synthetase from prostate cancer cellsRoss J Molinaro
Department of Chemistry, Cleveland State University, Euclid Avenue at East 24th Street, Cleveland, OH 44115, USA
Nucleic Acids Res 34:6684-95. 2006..Results suggest that OAS activation may occur in prostate cancer cells in vivo stimulated by cellular mRNAs for RKIP and PCBP2... - Effects of RNase L mutations associated with prostate cancer on apoptosis induced by 2',5'-oligoadenylatesYing Xiang
Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
Cancer Res 63:6795-801. 2003..Our findings support the notion that RNASEL mutations and some variants allow tumor cells to escape a potent apoptotic pathway... - Down-regulation of p53 by double-stranded RNA modulates the antiviral responseJoao T Marques
Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, OH 44195, USA
J Virol 79:11105-14. 2005..Hence, our data suggest that the down-regulation of p53 facilitates apoptosis, thereby limiting viral replication... - A natural human retrovirus efficiently complements vectors based on murine leukemia virusBeihua Dong
Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America
PLoS ONE 3:e3144. 2008..The similarity between the MLV and XMRV genomes suggests a possibility that the two viruses may interact when present in the same cell... - Human telomerase RNA degradation by 2'-5'-linked oligoadenylate antisense chimeras in a cell-free system, cultured tumor cells, and murine xenograft modelsJayashree M Paranjape
Department of Cancer Biology and Immunology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH, USA
Oligonucleotides 16:225-38. 2006.... - Activation of the interferon system by short-interfering RNAsCarol A Sledz
Department of Cancer Biology, Lerner Research Institute, NB40, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195 USA
Nat Cell Biol 5:834-9. 2003..Thus, siRNAs have broad and complicating effects beyond the selective silencing of target genes when introduced into cells. This is of critical importance, as siRNAs are currently being explored for their potential therapeutic use... - Mapping of the human RNASEL promoter and expression in cancer and normal cellsAimin Zhou
Department of Chemistry, Cleveland State University, Cleveland, OH 44115, USA
J Interferon Cytokine Res 25:595-603. 2005..Our findings are the first description of the human RNASEL promoter that allows constituitive expression in a range of normal and neoplastic cell types... - Protein kinase R-dependent regulation of interleukin-10 in response to double-stranded RNAArindam Chakrabarti
Departments of Cancer Biology and Molecular Cardiology, Lerner Research Institute, The Cleveland Clinic, Cleveland, OH 44195, USA
J Biol Chem 283:25132-9. 2008..Together, our data demonstrate the role of PKR in regulating an anti-inflammatory cytokine. The findings have significance in antiviral as well as broader innate immune responses... - Proteasome-mediated degradation of RNase L in response to phorbol-12-myristate-13-acetate (PMA) treatment of mouse L929 cellsBarbara I Chase
Department of Chemistry, Cleveland State University, Cleveland, OH 44115, USA
J Interferon Cytokine Res 23:565-73. 2003..These inhibitors completely blocked the degradation of RNase L caused by PMA. Our results show a novel regulatory pathway for RNase L that could have an impact on its antitumor and antiviral functions... - Androgen stimulates transcription and replication of xenotropic murine leukemia virus-related virusBeihua Dong
Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
J Virol 84:1648-51. 2010..Findings suggest that integration of the XMRV long terminal repeat (LTR) into host DNA could impart androgen stimulation on cellular genes... - Interferon-regulated pathways that control hepatitis B virus replication in transgenic miceLuca G Guidotti
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
J Virol 76:2617-21. 2002..Finally, RNase L(-/-) transgenic mice were undistinguishable from controls under basal conditions and after poly(I-C) or IFN-gamma administration, suggesting that RNase L does not modulate HBV replication in this model... - Functional replacement of the carboxy-terminal two-thirds of the influenza A virus NS1 protein with short heterologous dimerization domainsXiuyan Wang
Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
J Virol 76:12951-62. 2002..These results suggest that the main biological function of the carboxy-terminal region of the NS1 protein of influenza A virus is the enhancement of its IFN antagonist properties by stabilizing the NS1 dimeric structure... - Diverse functions of RNase L and implications in pathologyCatherine Bisbal
IGH UPR CNRS 1142, 141 rue de la Cardonille, 34396 Montpellier, France
Biochimie 89:789-98. 2007..Several recent studies have suggested that RNase L could have a role in cancer biology and evidence of a tumor suppressor function of RNase L has emerged from studies on the genetics of hereditary prostate cancer... - Integration site preference of xenotropic murine leukemia virus-related virus, a new human retrovirus associated with prostate cancerSanggu Kim
Biomedical Engineering Interdepartmental Program, UCLA School of Medicine, Los Angeles, CA 90095, USA
J Virol 82:9964-77. 2008..These results are consistent with a model in which XMRV may contribute to tumorigenicity via a paracrine mechanism... - RNase L plays a role in the antiviral response to West Nile virusSvetlana V Scherbik
Department of Biology, Georgia State University, P O Box 4010, Atlanta, Georgia 30302 4010, USA
J Virol 80:2987-99. 2006..The results suggest that RNase L activation is not a major component of the Oas1b-mediated flavivirus resistance phenotype... - Endowing RNase H-inactive antisense with catalytic activity: 2-5A-morphantsLonghu Zhou
Department of Chemistry, Northern Arizona University, Box 5698, Flagstaff, Arizona 86011-5698, USA
Bioconjug Chem 16:383-90. 2005..RNase L assays demonstrated that this novel 2-5A-antisense chimera had significant biological activity, thereby providing another potential tool for RNA ablation... - Distinctive roles for 2',5'-oligoadenylate synthetases and double-stranded RNA-dependent protein kinase R in the in vivo antiviral effect of an adenoviral vector expressing murine IFN-betaKhaldun Al-Khatib
Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
J Immunol 172:5638-47. 2004.... - RNase L mediates transient control of the interferon response through modulation of the double-stranded RNA-dependent protein kinase PKRKhalid S A Khabar
Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Center, Riyadh 11211, Saudi Arabia
J Biol Chem 278:20124-32. 2003..These results suggest a role for RNase L in the transient control of the IFN response and possibly of other cytokine and stress responses... - ISG20, a new interferon-induced RNase specific for single-stranded RNA, defines an alternative antiviral pathway against RNA genomic virusesLucile Espert
UMR CNRS 5094, EFS, 240 avenue Emile Jeanbrau, 34094 Montpellier Cedex 5, France
J Biol Chem 278:16151-8. 2003..We thus show that, besides RNase L, ISG20 has an antiviral activity, supporting the idea that it might represent a novel antiviral pathway in the mechanism of IFN action... - RNA-dependent protein kinase is required for alpha-1 interferon transgene-induced resistance to genital herpes simplex virus type 2Daniel J J Carr
Department of Ophthalmology, DMEI 415, The University of Oklahoma Health Sciences Center, 608 Stanton L Young Blvd, Oklahoma City, OK 73104, USA
J Virol 79:9341-5. 2005.... - Critical role for the oligoadenylate synthetase/RNase L pathway in response to IFN-beta during acute ocular herpes simplex virus type 1 infectionBobbie Ann Austin
Department of Ophthalmology, University of Oklahoma Health Sciences Center, Biomedical Sciences Building, 608 Stanton L Young Boulevard, Oklahoma City, OK 73104, USA
J Immunol 175:1100-6. 2005.... - Identification of a novel Gammaretrovirus in prostate tumors of patients homozygous for R462Q RNASEL variantAnatoly Urisman
Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, United States of America
PLoS Pathog 2:e25. 2006..These findings also raise questions about the possible relationship between exogenous infection and cancer development in genetically susceptible individuals... - PKR and RNase L contribute to protection against lethal West Nile Virus infection by controlling early viral spread in the periphery and replication in neuronsMelanie A Samuel
Department of Molecular Microbiology, Washington University School of Medicine, 660 S Euclid Ave, St Louis, MO 63110, USA
J Virol 80:7009-19. 2006..Our data suggest that PKR and RNase L contribute to IFN-mediated protection in a cell-restricted manner and control WNV infection in peripheral tissues and some neuronal subtypes... - The lack of RNA-dependent protein kinase enhances susceptibility of mice to genital herpes simplex virus type 2 infectionDaniel J J Carr
Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
Immunology 118:520-6. 2006..Collectively, activation of PKR, but not of OAS, contributes significantly to the local control and spread of HSV-2 following genital infection... - Interferon-alpha-induced expression of phospholipid scramblase 1 through STAT1 requires the sequential activation of protein kinase Cdelta and JNKKe Wen Zhao
Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis, Chinese Ministry of Education, Rui Jin Hospital, Shanghai Jiaotong University School of Medicine formerly Shanghai Second Medical University, Shanghai 200025, China
J Biol Chem 280:42707-14. 2005..In conclusion, our results suggest that the induction of PLSCR1 transcription through STAT1 depends upon sequential activation of PKCdelta and JNK... - An alternative spliced RNASEL variant in peripheral blood leukocytesHanna Rennert
Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, NY 10012, USA
J Interferon Cytokine Res 26:820-6. 2006..Our results suggest that RNase L plays an important role in granulocytes as an innate immunity enzyme that controls viral infections... - RNase L activity does not contribute to host RNA degradation induced by herpes simplex virus infectionTracy J Smith
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, Box 8096, 660 S. Euclid Avenue, St Louis, MO 63110, USA
J Gen Virol 84:925-8. 2003..Furthermore, the data show that HSV-1 does not significantly induce RNase L activity in murine embryo fibroblasts... - The murine double-stranded RNA-dependent protein kinase PKR and the murine 2',5'-oligoadenylate synthetase-dependent RNase L are required for IFN-beta-mediated resistance against herpes simplex virus type 1 in primary trigeminal ganglion cultureKhaldun Al-Khatib
Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
Virology 313:126-35. 2003.... - Alphavirus-based DNA vaccine breaks immunological tolerance by activating innate antiviral pathwaysWolfgang W Leitner
National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
Nat Med 9:33-9. 2003.... - Synthesis, characterization, and biological properties of 8-azido- and 8-amino-substituted 2',5'-oligoadenylatesHiroaki Sawai
Department of Applied Chemistry, Faculty of Engineering, Gunma University, Kiryu, Gunma 376 8515 Japan
J Med Chem 46:4926-32. 2003..In addition, these 8-azidoadenosine compounds may be considered as candidates for experimental therapeutic agents because they have enhanced stability to enzyme degradation while retaining the ability to activate RNase L... - Type I interferon inhibits antibody responses induced by a chimpanzee adenovirus vectorScott E Hensley
Cell and Molecular Biology Group, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
Mol Ther 15:393-403. 2007..We conclude that type I IFN, when induced at high levels, can downregulate transgene product expression of Ad vectors and inhibit the formation of optimal antibody responses... - Dichotomy between survival and lytic gene expression in RNase L- and PKR-deficient mice transduced with an adenoviral vector expressing murine IFN-beta following ocular HSV-1 infectionKhaldun Al-Khatib
Department of Ophthalmology, Microbiology, and Immunology, University of Oklahoma Health Sciences Center, HSC608 Stanton L Young Blvd, Oklahoma City, OK 73104, USA
Exp Eye Res 80:167-73. 2005..Collectively, the data suggest survival rates of viral-infected mice do not reflect the replication capacity as measured by herpes simplex virus type one lytic gene expression... - Involvement of the interferon-regulated antiviral proteins PKR and RNase L in reovirus-induced shutoff of cellular translationJennifer A Smith
Department of Microbiology, University of Minnesota, 420 Delaware St, Minneapolis, MN 55455, USA
J Virol 79:2240-50. 2005..In addition, our results suggest that reovirus has evolved effective mechanisms to avoid the actions of the interferon-stimulated antiviral pathways that include PKR and RNase L and may even benefit from their expression... - Sensitivity of hepatitis C virus RNA to the antiviral enzyme ribonuclease L is determined by a subset of efficient cleavage sitesJian-Qiu Han
Department of Microbiology, University of Colorado Health Sciences Center, Denver, CO 80262, USA
J Interferon Cytokine Res 24:664-76. 2004..Our data indicate that a relatively small number of the UA and UU dinucleotides in HCV RNA mediate the overall sensitivity of HCV RNA to cleavage by RNase L... - A viral RNA competitively inhibits the antiviral endoribonuclease domain of RNase LHannah L Townsend
Department of Microbiology, University of Colorado Denver, School of Medicine, Aurora, Colorado 80045, USA
RNA 14:1026-36. 2008..Consistent with the kinetic profile of a competitive inhibitor, the viral RNA inhibited the constitutively active endoribonuclease domain of RNase L. We call this viral RNA the RNase L competitive inhibitor RNA (RNase L ciRNA)... - A phylogenetically conserved RNA structure in the poliovirus open reading frame inhibits the antiviral endoribonuclease RNase LJian Qiu Han
Department of Microbiology, School of Medicine, University of Colorado, Mail Stop 8333, Room P18 9116, 12800 E 19th Ave, Aurora, CO 80045, USA
J Virol 81:5561-72. 2007.... - A convenient and sensitive fluorescence resonance energy transfer assay for RNase L and 2',5' oligoadenylatesChandar S Thakur
Department of Chemistry, Cleveland State University, OH, USA
Methods Mol Med 116:103-13. 2005..The RNA probe used in the FRET assay was designed based on a region of respiratory syncytial genomic RNA. We demonstrate the utility of our FRET assay with several novel biostable analogs of 2-5A... - Delivery of 2-5A cargo into living cells using the Tat cell penetrating peptide: 2-5A-tatLonghu Zhou
Department of Chemistry and Biochemistry, Northern Arizona University, Flagstaff, AZ 86011-5698, USA
Bioorg Med Chem 14:7862-74. 2006..This strategy provides a valuable vehicle for the entry of the charged 2-5A molecule into cells and may provide a means for targeted destruction of HIV RNA in vivo... - Alphavirus minus-strand synthesis and persistence in mouse embryo fibroblasts derived from mice lacking RNase L and protein kinase RDorothea L Sawicki
Department of Microbiology and Immunology, Medical College of Ohio, Toledo, Ohio 43614, USA
J Virol 77:1801-11. 2003.... - Hepatitis C virus RNA: dinucleotide frequencies and cleavage by RNase LChristopher L Washenberger
Department of Microbiology, University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045, United States
Virus Res 130:85-95. 2007..Thus, RNase L can cleave HCV RNAs efficiently and variably reduced frequencies of UA and UU dinucleotides in HCV RNA ORFs are consistent with the selective pressure of RNase L... - Absence of PKR attenuates the anti-HSV-1 activity of an adenoviral vector expressing murine IFN-betaKhaldun Al-Khatib
Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
J Interferon Cytokine Res 22:861-71. 2002....
Research Grants
- Antiviral Mechanisms of 2-5A Dependent RNase LRobert H Silverman; Fiscal Year: 2010..The knowledge to be gained may thus lead to strategies for controlling important human viral pathogens. Therefore, there are cogent and health-related justifications for these studies. ..
- Role of Phospholipid Scramblase in Interferon ActionRobert Silverman; Fiscal Year: 2005..abstract_text> ..
- Antiviral Mechanisms of 2 5A Dependent RNase LRobert Silverman; Fiscal Year: 2006..Therefore, continuation of this PROJECT is expected to lead to the eventual development of novel strategies for treatment of viral infections and cancer. ..
- Role of RNase L in the Biology of Prostate CancerRobert Silverman; Fiscal Year: 2007..The experiments described in this PROPOSAL describe a previously unexplored area of research that may bring us closer to the eventual goal of understanding a molecular basis for prostate cancer. ..
- Antiviral Mechanisms of 2-5A Dependent RNase LRobert Silverman; Fiscal Year: 2007..The knowledge to be gained may thus lead to strategies for controlling important human viral pathogens. Therefore, there are cogent and health-related justifications for these studies. ..
- Antiviral Mechanisms of 2-5A Dependent RNase LRobert Silverman; Fiscal Year: 2009..The knowledge to be gained may thus lead to strategies for controlling important human viral pathogens. Therefore, there are cogent and health-related justifications for these studies. ..
- REGULATION OF 2-5A-DEPENDENT RNASE LEVELS OF INTERFERONRobert Silverman; Fiscal Year: 1993..Because the 2-5A system has the ability to inhibit both virus replication and neoplastic cell growth this project directly impacts on the control of virus infection and cancer in man...
- ANTIVIRAL MECHANISMS OF 2 5A DEPENDENT RNASERobert Silverman; Fiscal Year: 2001..This project may lead to the discovery and development of novel therapeutic agents for viral infections, cancer and other diseases. ..