Takhar Kasumov

Summary

Affiliation: Cleveland Clinic Foundation
Country: USA

Publications

  1. pmc Assessment of cardiac proteome dynamics with heavy water: slower protein synthesis rates in interfibrillar than subsarcolemmal mitochondria
    Takhar Kasumov
    Department of Gastroenterology and Hepatology, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Am J Physiol Heart Circ Physiol 304:H1201-14. 2013
  2. pmc Dipropionylcysteine ethyl ester compensates for loss of citric acid cycle intermediates during post ischemia reperfusion in the pig heart
    Takhar Kasumov
    Department Nutrition, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Cardiovasc Drugs Ther 23:459-69. 2009
  3. pmc Quantification of ceramide species in biological samples by liquid chromatography electrospray ionization tandem mass spectrometry
    Takhar Kasumov
    Department of Gastroenterology and Hepatology, Cleveland Clinic, Cleveland, OH 44195, USA
    Anal Biochem 401:154-61. 2010
  4. pmc Measuring protein synthesis using metabolic ²H labeling, high-resolution mass spectrometry, and an algorithm
    Takhar Kasumov
    Department of Gastroenterology, Cleveland Clinic, Cleveland, OH 44195, USA
    Anal Biochem 412:47-55. 2011
  5. pmc Probing peroxisomal beta-oxidation and the labelling of acetyl-CoA proxies with [1-(13C)]octanoate and [3-(13C)]octanoate in the perfused rat liver
    Takhar Kasumov
    Department of Nutrition, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
    Biochem J 389:397-401. 2005
  6. pmc Competition between acetate and oleate for the formation of malonyl-CoA and mitochondrial acetyl-CoA in the perfused rat heart
    Fang Bian
    Department of Nutrition, Case Western Reserve University, 2109 Adelbert Road, Room BRB923, Cleveland, OH 44106 4906, USA
    J Mol Cell Cardiol 41:868-75. 2006
  7. pmc Metabolomic and mass isotopomer analysis of liver gluconeogenesis and citric acid cycle. I. Interrelation between gluconeogenesis and cataplerosis; formation of methoxamates from aminooxyacetate and ketoacids
    Lili Yang
    Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 283:21978-87. 2008
  8. pmc Plasma proteome dynamics: analysis of lipoproteins and acute phase response proteins with 2H2O metabolic labeling
    Ling Li
    Department of Research Core Services, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Mol Cell Proteomics 11:M111.014209. 2012
  9. pmc Mass isotopomer study of anaplerosis from propionate in the perfused rat heart
    Takhar Kasumov
    Department of Nutrition, Case Western Reserve University, School of Medicine WG 48, 10900 Euclid Avenue, Cleveland, OH 44106 4954, USA
    Arch Biochem Biophys 463:110-7. 2007
  10. pmc Interrelations between C4 ketogenesis, C5 ketogenesis, and anaplerosis in the perfused rat liver
    Shuang Deng
    Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 284:27799-807. 2009

Collaborators

Detail Information

Publications37

  1. pmc Assessment of cardiac proteome dynamics with heavy water: slower protein synthesis rates in interfibrillar than subsarcolemmal mitochondria
    Takhar Kasumov
    Department of Gastroenterology and Hepatology, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Am J Physiol Heart Circ Physiol 304:H1201-14. 2013
    ....
  2. pmc Dipropionylcysteine ethyl ester compensates for loss of citric acid cycle intermediates during post ischemia reperfusion in the pig heart
    Takhar Kasumov
    Department Nutrition, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Cardiovasc Drugs Ther 23:459-69. 2009
    ....
  3. pmc Quantification of ceramide species in biological samples by liquid chromatography electrospray ionization tandem mass spectrometry
    Takhar Kasumov
    Department of Gastroenterology and Hepatology, Cleveland Clinic, Cleveland, OH 44195, USA
    Anal Biochem 401:154-61. 2010
    ..This technique could be extended for quantification of other ceramides and sphingolipids with no significant modification...
  4. pmc Measuring protein synthesis using metabolic ²H labeling, high-resolution mass spectrometry, and an algorithm
    Takhar Kasumov
    Department of Gastroenterology, Cleveland Clinic, Cleveland, OH 44195, USA
    Anal Biochem 412:47-55. 2011
    ..As a proof of principle, we demonstrate that this approach can detect the effect of nutritional status on albumin synthesis in rats given (2)H(2)O...
  5. pmc Probing peroxisomal beta-oxidation and the labelling of acetyl-CoA proxies with [1-(13C)]octanoate and [3-(13C)]octanoate in the perfused rat liver
    Takhar Kasumov
    Department of Nutrition, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
    Biochem J 389:397-401. 2005
    ....
  6. pmc Competition between acetate and oleate for the formation of malonyl-CoA and mitochondrial acetyl-CoA in the perfused rat heart
    Fang Bian
    Department of Nutrition, Case Western Reserve University, 2109 Adelbert Road, Room BRB923, Cleveland, OH 44106 4906, USA
    J Mol Cell Cardiol 41:868-75. 2006
    ..The inhibition by acetate or propionate of acetyl-CoA production from oleate probably results from a competition for mitochondrial CoA between the CoA-utilizing enzymes...
  7. pmc Metabolomic and mass isotopomer analysis of liver gluconeogenesis and citric acid cycle. I. Interrelation between gluconeogenesis and cataplerosis; formation of methoxamates from aminooxyacetate and ketoacids
    Lili Yang
    Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 283:21978-87. 2008
    ..These adducts may exert metabolic effects unrelated to their effect on gluconeogenesis...
  8. pmc Plasma proteome dynamics: analysis of lipoproteins and acute phase response proteins with 2H2O metabolic labeling
    Ling Li
    Department of Research Core Services, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Mol Cell Proteomics 11:M111.014209. 2012
    ..We conclude that this technique can effectively measure the synthesis of plasma proteins and can be used to study the regulation of protein homeostasis under physiological and pathological conditions...
  9. pmc Mass isotopomer study of anaplerosis from propionate in the perfused rat heart
    Takhar Kasumov
    Department of Nutrition, Case Western Reserve University, School of Medicine WG 48, 10900 Euclid Avenue, Cleveland, OH 44106 4954, USA
    Arch Biochem Biophys 463:110-7. 2007
    ..Under our conditions, [(13)C(3)]propionate was a purely anaplerotic substrate since there was no labeling of mitochondrial acetyl-CoA, reflected by the labeling of the acetyl moiety of citrate...
  10. pmc Interrelations between C4 ketogenesis, C5 ketogenesis, and anaplerosis in the perfused rat liver
    Shuang Deng
    Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 284:27799-807. 2009
    ..Anaplerosis and gluconeogenesis from heptanoate are inhibited by octanoate. The data have implications for the design of diets for the treatment of long chain fatty acid oxidation disorders, such as the triheptanoin-based diet...
  11. pmc Reduced cardiovascular risk after bariatric surgery is linked to plasma ceramides, apolipoprotein-B100, and ApoB100/A1 ratio
    Helen M Heneghan
    Bariatric and Metabolic Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Surg Obes Relat Dis 9:100-7. 2013
    ..We hypothesized that the beneficial effect of Roux-en-Y gastric bypass (RYGB) on CVD risk is related to ceramide-mediated improvement in lipoprotein profile...
  12. ncbi request reprint In vitro modeling of fatty acid synthesis under conditions simulating the zonation of lipogenic [13C]acetyl-CoA enrichment in the liver
    Ilya R Bederman
    Department of Nutrition and Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 279:43217-26. 2004
    ..ISA uses all mass isotopomers, correctly identified imposed gradients of precursor enrichment, and estimated the expected values of fractional synthesis within the constraints of the data...
  13. ncbi request reprint Peroxisomal and mitochondrial oxidation of fatty acids in the heart, assessed from the 13C labeling of malonyl-CoA and the acetyl moiety of citrate
    Fang Bian
    Department of Nutrition, Case Western Reserve University, Cleveland Ohio 44106, USA
    J Biol Chem 280:9265-71. 2005
    ....
  14. pmc Metabolomic and mass isotopomer analysis of liver gluconeogenesis and citric acid cycle: II. Heterogeneity of metabolite labeling pattern
    Lili Yang
    Departments of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 283:21988-96. 2008
    ..Our data have implications for the use of labeling patterns for the calculation of metabolic rates or fractional syntheses in liver, as well as for modeling liver intermediary metabolism...
  15. ncbi request reprint Regulation of malonyl-CoA concentration and turnover in the normal heart
    Aneta E Reszko
    Department of Biochemistry, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
    J Biol Chem 279:34298-301. 2004
    ..Our data show that, in the absence of enzyme inhibitors, the rate of acetyl-CoA carboxylation is the main determinant of the malonyl-CoA concentration in the heart...
  16. pmc An improved measurement of isotopic ratios by high resolution mass spectrometry
    Serguei Ilchenko
    Center for Proteomics and Bioinformatics, Case Western Reserve University, Cleveland, OH 44106, USA
    J Am Soc Mass Spectrom 24:309-12. 2013
    ..Overall, this technique allows accurate measurements of the isotopic ratios of proteolytic peptides, a critical step for enabling routine studies of proteome dynamics...
  17. ncbi request reprint Assessing the reversibility of the anaplerotic reactions of the propionyl-CoA pathway in heart and liver
    Aneta E Reszko
    Department of Biochemistry, Nutrition, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 278:34959-65. 2003
    ..The thermodynamic dis-equilibrium of the combined reactions of the propionyl-CoA pathway explains the effectiveness of anaplerosis from propionyl-CoA precursors such as heptanoate...
  18. ncbi request reprint Zonation of labeling of lipogenic acetyl-CoA across the liver: implications for studies of lipogenesis by mass isotopomer analysis
    Ilya R Bederman
    Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 279:43207-16. 2004
    ..R., Kasumov, T., Reszko, A. E., David, F., Brunengraber, H., and Kelleher, J. K. (2004) J. Biol. Chem. 279, 43217-43226)...
  19. pmc Simultaneous assay of isotopic enrichment and concentration of guanidinoacetate and creatine by gas chromatography-mass spectrometry
    Takhar Kasumov
    Department of Pathobiology, Lerner Research Institute, Cleveland Clinic, OH 44195, USA
    Anal Biochem 395:91-9. 2009
    ..00097), suggesting a large pool size and a slow rate of synthesis of creatine. This method can be used to estimate the rate of synthesis of creatine in vivo in human and animal studies...
  20. ncbi request reprint Parenteral and enteral metabolism of anaplerotic triheptanoin in normal rats
    Renee P Kinman
    Department of Pediatrics, Case Western Reserve University, Euclid Ave, Cleveland, OH 44106 4954, USA
    Am J Physiol Endocrinol Metab 291:E860-6. 2006
    ..During intraduodenal infusion of triheptanoin, lipolysis was not stimulated. Our data support the hypothesis that intravenous triheptanoin could be used to treat decompensated patients with long-chain fatty acid oxidation disorders...
  21. ncbi request reprint Peroxisomal fatty acid oxidation is a substantial source of the acetyl moiety of malonyl-CoA in rat heart
    Aneta E Reszko
    Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 279:19574-9. 2004
    ..In addition, this pathway may supply some acyl groups used in protein acylation, which is increasingly recognized as an important regulatory mechanism for many biochemical processes...
  22. pmc Plasma levels of asymmetric dimethylarginine in patients with biopsy-proven nonalcoholic fatty liver disease
    Takhar Kasumov
    Department of Gastroenterology, Cleveland Clinic, Cleveland, OH 44195, USA
    Metabolism 60:776-81. 2011
    ..027). Elevated plasma concentrations of ADMA in biopsy-proven NAFLD were primarily related to insulin resistance. Hepatic dysfunction in NAFLD does not appear to make significant contribution to changes in plasma methylarginine levels...
  23. doi request reprint 2H2O-based high-density lipoprotein turnover method for the assessment of dynamic high-density lipoprotein function in mice
    Takhar Kasumov
    Department of Gastroenterology and Hepatology, Case Western Reserve University School of Medicine, Cleveland Clinic, Cleveland, OH 44195, USA
    Arterioscler Thromb Vasc Biol 33:1994-2003. 2013
    ....
  24. pmc Measuring proteome dynamics in vivo: as easy as adding water?
    Nadia Rachdaoui
    Department Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA
    Mol Cell Proteomics 8:2653-63. 2009
    ..g. following a meal), respectively. We expect that the use of labeled water is applicable to wide scale investigations of proteome dynamics and can therein be used to obtain a functional image of gene expression in vivo...
  25. pmc Role of ceramides in nonalcoholic fatty liver disease
    Mangesh Pagadala
    Department of Gastroenterology Hepatology, Cleveland Clinic, Cleveland, OH 44195, USA
    Trends Endocrinol Metab 23:365-71. 2012
    ..A better understanding of the role of ceramides in steatohepatitis has both diagnostic and therapeutic implications for the treatment of fatty liver disease...
  26. ncbi request reprint New secondary metabolites of phenylbutyrate in humans and rats
    Takhar Kasumov
    Department of Nutrition, Room 280, Case Western Reserve University, 11000 Cedar Rd, Cleveland, OH 44106 7139, USA
    Drug Metab Dispos 32:10-9. 2004
    ..These are partially bioavailable in rats and could be used to administer large doses of phenylbutyrate in a sodium-free, noncaustic form...
  27. pmc Gastric bypass surgery reduces plasma ceramide subspecies and improves insulin sensitivity in severely obese patients
    Hazel Huang
    Department of Pathobiology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA
    Obesity (Silver Spring) 19:2235-40. 2011
    ..62, P = 0.04). We conclude that there is a potential role for ceramide lipids as mediators of the proinflammatory state and improved insulin sensitivity after gastric bypass surgery...
  28. pmc Glycine and urea kinetics in nonalcoholic steatohepatitis in human: effect of intralipid infusion
    Srinivasan Dasarathy
    Department of Gastroenterology and Hepatology, Lerner Research Institute, Cleveland, USA
    Am J Physiol Gastrointest Liver Physiol 297:G567-75. 2009
    ..An increase in oxidative stress, induced by a higher rate of fatty acid oxidation in NASH, may have caused an increase in the contribution of serine to cystathionine to meet the higher demands for glutathione...
  29. ncbi request reprint Quantification of malonyl-coenzyme A in tissue specimens by high-performance liquid chromatography/mass spectrometry
    Paul E Minkler
    Louis Stokes Department of Veterans Affairs Medical Center, Medical Research Service, Cleveland, OH 44106, USA
    Anal Biochem 352:24-32. 2006
    ..9+/-0.6, 1.3+/-0.4, and 0.7+/-0.2nmol/g wet weight, respectively, for these tissues. This analytical approach can be extended to the quantification of other acyl-CoA species with no significant modification...
  30. ncbi request reprint Assay of the activity of malonyl-coenzyme A decarboxylase by gas chromatography-mass spectrometry
    Xiao Wang
    Department of Nutrition, Case Western Reserve University, Cleveland, OH 44106, USA
    Anal Biochem 363:169-74. 2007
    ..80+/-0.41, 2.59+/-0.37 (P<0.05), and 3.07+/-0.70 (P<0.05), respectively. We report a practical, nonradioactive, sensitive assay of MCD in crude tissue extract...
  31. pmc Catabolism of 4-hydroxyacids and 4-hydroxynonenal via 4-hydroxy-4-phosphoacyl-CoAs
    Guo Fang Zhang
    Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 284:33521-34. 2009
    ..Our data illustrate the potential of the combination of metabolomics and mass isotopomer analysis for pathway discovery...
  32. pmc Plasma ceramides are elevated in obese subjects with type 2 diabetes and correlate with the severity of insulin resistance
    Jacob M Haus
    Department of Pathobiology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA
    Diabetes 58:337-43. 2009
    ..Ceramides are a putative mediator of insulin resistance and lipotoxicity, and accumulation of ceramides within tissues in obese and diabetic subjects has been well described...
  33. ncbi request reprint Post-ischemic treatment with dipyruvyl-acetyl-glycerol decreases myocardial infarct size in the pig
    William C Stanley
    Departments of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Cardiovasc Drugs Ther 17:209-16. 2003
    ..05)), with no difference in blood pressure or heart rate between groups. In conclusion, an intravenous infusion of DPAG safely increases arterial pyruvate concentration and reduces myocardial infarct size following myocardial ischemia...
  34. ncbi request reprint Assay of the concentration and (13)C isotopic enrichment of propionyl-CoA, methylmalonyl-CoA, and succinyl-CoA by gas chromatography-mass spectrometry
    Takhar Kasumov
    Department and Nutrition, Case Western Reserve University, 11000 Cedar Road, Cleveland, OH 44106 7139, USA
    Anal Biochem 305:90-6. 2002
    ..Thus, our assays allow measuring the turnover of the coenzyme A derivatives involved in anaplerosis of the citric acid cycle via precursors of propionyl-CoA, i.e., propionate, odd-chain fatty acids, isoleucine, threonine, and valine...
  35. pmc Sarcopenia and a physiologically low respiratory quotient in patients with cirrhosis: a prospective controlled study
    Cathy Glass
    Department of Nutrition Therapy, Digestive Disease Institute, Cleveland Clinic, Cleveland, Ohio, USA
    J Appl Physiol (1985) 114:559-65. 2013
    ..Although low RQ does not predict short-term mortality in these patients, it may reflect a decompensated metabolic state that requires careful nutritional management with appropriate consideration for preservation of skeletal muscle mass...
  36. ncbi request reprint Identification of phenylbutyrylglutamine, a new metabolite of phenylbutyrate metabolism in humans
    Blandine Comte
    Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Mass Spectrom 37:581-90. 2002
    ..Thus, additional metabolites of phenylbutyrate are yet to be identified...
  37. ncbi request reprint Influence of diet on the modeling of adipose tissue triglycerides during growth
    Daniel Z Brunengraber
    Department of Mathematics, Case Western Reserve University School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106 4906, USA
    Am J Physiol Endocrinol Metab 285:E917-25. 2003
    ..2H2O can be used to examine the mechanisms responsible for the accumulation of triglycerides, e.g., factors that affect 1) triglyceride synthesis and degradation and 2) the source of fatty acids that are used in esterification...