- Successful pyrosequencing of GC-rich DNA sequences by partial substitution of deoxyguanosine with deoxyinosineLulette Tricia C Bravo
Departments of Infectious Disease, Cleveland Clinic, Cleveland, OH, USA
Diagn Mol Pathol 19:123-5. 2010..This paper describes the successful use of a modified pyrosequencing protocol through partial substitution of deoxyguanosine triphosphate with deoxyinosine triphosphate in the polymerase chain reaction...
- Pyrosequencing for rapid detection of Mycobacterium tuberculosis resistance to rifampin, isoniazid, and fluoroquinolonesLulette Tricia C Bravo
Cleveland Clinic, Cleveland, Ohio 44195, USA
J Clin Microbiol 47:3985-90. 2009..3%, 63.8% and 100%, and 70.0% and 100% for the detection of resistance to rifampin, isoniazid, and ofloxacin, respectively. Pyrosequencing is thus a rapid and accurate method for detecting M. tuberculosis resistance to these three drugs...